共查询到17条相似文献,搜索用时 57 毫秒
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应用实时定量PCR方法测定样品中转基因玉米Bt176的含量,并从多个方面来分析测量的不确定度。结果显示,A类不确定度为0.0003,B类不确定度为0.0007,合成不确定度为0.001,有效自由度veff为272,包含概率P为95%,扩展不确定度U=0.002,测量结果为(0.8±0.2)%。不确定度评估结果表明,测量不确定度的主要来源是微量液体转移量的偏差。 相似文献
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玉米Bt转基因植株抗虫性鉴定研究初报 总被引:5,自引:2,他引:5
经室内喂虫和田间接种,鉴定了Mo17,478等4个常用自交及其与91-1-8杂交的抗虫转基因杂种F1的抗虫性。结果表明,不同转基因材料之间以及同一转基因材料的不同单株间抗虫性差异很大,有少量植株表现高度抗性,个别植株几乎没有抗性。 相似文献
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玉米Bt转基因植株抗虫性量化分析 总被引:4,自引:0,他引:4
对87-1,8085,综3,N808等4个回交一代Bt转基因材料进行田间抗虫性接卵鉴定的结果表明,成株期转基因材料群体茎秆的平抗虫性对照提高45%以上,差异达或极显著水平,叶片的抗虫性提高30%以上,不同遗传背景的转基因材料,抗性表现不一,回交一代群体内,不同单株的抗虫性差异悬殊,均存在高抗到抗感不等的分离。 相似文献
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Bt棉抗虫性遗传研究 总被引:2,自引:0,他引:2
以Bt棉R-1、R-2及常规品种鄂棉22、鄂抗棉3号为亲本配制了3个组合的F1、F2和测交后代,了各组合的抗虫性遗传。结果表明,R1与鄂棉22、R-2与鄂抗棉3号分别存在一对显性基因差异,R-1与R-2的Bt不等位。外源Bt抗虫基因导入棉花后能稳定遗传,但插入位点是随机的。 相似文献
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双抗夹心荧光免疫吸附测定法(sFLISA)定量检测转基因玉米中Bt蛋白 总被引:1,自引:0,他引:1
将量子点(Quantum Dots,QDs)应用于标记羊抗兔多克隆抗体,并利用Bt杀虫晶体蛋白作为标准蛋白和免疫抗原,通过抗体-抗原-抗体-量子点标记抗体反应,建立了双抗夹心荧光免疫吸附测定法(sFLISA),以定量检测转基因玉米中的Bt表达蛋白.研究结果表明,该方法的最低检测限为3 pg/mL,线性检测范围为6~200 pg/mL,回收率在90.0%~105.9%,变异系数在3.0%~12.6%. 相似文献
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转基因生物技术本身同任何新技术一样.既可造福人类,又可能存在安全隐患.关键是人类如何避害趋利,如何把它给人类可能带来的风险降到最低水平。转基因技术发展过程中的潜在风险一直为各方面所高度关注。 相似文献
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Ecological and population biology issues constitute serious challenges to the application of genetically modified mosquitos (GMM) for disease control. 相似文献
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抗虫转基因作物研究进展 总被引:2,自引:0,他引:2
简要介绍了转基因作物 (GMO)的发展概况 ,对近年来国内外抗虫 GMO在转 B.t.毒素蛋白GMO;转蛋白酶抑制剂 GMO;转植物凝集素 GMO;其他类型抗虫 GMO;抗虫的基因沉默与失活现象等 5个方面的研究进展进行了论述。最后 ,对抗虫 GMO的发展前景进行了展望。 相似文献
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Perry Bradbury HACKETT 《农业科学与工程前沿(英文版)》2020,7(2):188-203
Precision genetics and breeding have the potential to meet the agricultural needs and goals of the world in the 21st century. These needs include increasing the efficiency of production of animals and improving their products with minimal impact on the environment. The USA is the major innovator in genomic science and the acknowledged leader in formulating policies to regulate genetic applications in medicine and agriculture. However, governments worldwide have been exceedingly reluctant to support the introduction of genetically modified (GM) animals into agriculture. Regulatory policies have stagnated due to legal guidelines that could not anticipate the needs and solutions that are evident today. This must change if we are to maintain planetary integrity. I propose a new, market-based regulatory model for GM livestock that has both a strong scientific foundation and has worked for 10000 years. The model is similar to that for information technology in which specific algorithms drive computer and cell phone applications. Genome engineers write genetic algorithms that drive the traits in biological organisms. Accordingly, GM products should be viewed in terms of their use and public benefit rather than by limitations to the genetic programing coming from a few highly vocal groups. Genetic algorithms (Genapps) of the 21st century will include not only introduction of synthetic genes, but also complete natural and synthetic biochemical pathways to produce agricultural products that are maximally efficient, healthy to humans and animals, and sustainable in an era of changing climates while avoiding environmental degradation. 相似文献
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Engineering modified Bt toxins to counter insect resistance 总被引:2,自引:0,他引:2
Soberón M Pardo-López L López I Gómez I Tabashnik BE Bravo A 《Science (New York, N.Y.)》2007,318(5856):1640-1642
The evolution of insect resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxins that are widely used in sprays and transgenic crops. Resistance to Bt toxins in some insects is linked with mutations that disrupt a toxin-binding cadherin protein. We show that susceptibility to the Bt toxin Cry1Ab was reduced by cadherin gene silencing with RNA interference in Manduca sexta, confirming cadherin's role in Bt toxicity. Native Cry1A toxins required cadherin to form oligomers, but modified Cry1A toxins lacking one alpha-helix did not. The modified toxins killed cadherin-silenced M. sexta and Bt-resistant Pectinophora gossypiella that had cadherin deletion mutations. Our findings suggest that cadherin promotes Bt toxicity by facilitating toxin oligomerization and demonstrate that the modified Bt toxins may be useful against pests resistant to standard Bt toxins. 相似文献