分光光度法检测微量甲醛和长效清应用过程中甲醛含量变化

采用乙酰丙酮分光光度法测定水中微量甲醛的含量。本法在４１４ｎｍ波长处有最大吸收，吸收值和甲醛含量之间有良好的相关关系，相关系数ｒ为０．９９９９９，用于样品检测的相对平均偏差小于２％，加标回收率在９５％－１０５％之间。显色可稳定达１小时。用本法检测长效清（复方甲醛）应用过程中甲醛浓度的变化，发现甲醛含量始终在其最初浓度的±１０％以内，非常稳定     

兽用去势液中甲醛含量的测定

本文介绍了去势液中甲醛含量测定的两种方法，水蒸汽蒸馏－亚硫酸钠吸收－酸滴定法和盐酸羟胺法。平均回收率分别为９９．３％、９７．８％，变异系数分别为０．７８％、１．１６％。其中蒸馏法也适用于可通过蒸馏分离出甲醛的任何样品，具有通用性，方法准确可靠。     

乙酰丙酮分光光度法测定甘油缩甲醛中游离甲醛含量

乙酰丙酮与甲醛在乙酸-乙酸铵介质中形成黄色化合物,该化合物在411 nm波长处有最大吸收。甲醛在比色液中含量小于4μg/mL时,符合比尔定律,线性相关系数R2=0.999 9。对样品平行6次测定相对标准偏差为0.68%~1.62%,回收率为91.2%~93.8%。该方法测定甘油缩甲醛中游离甲醛含量简便、快捷,准确可靠。     

避免疫苗中有色物质干扰的甲醛残留量测定法

建立一种能有效避免疫苗中有色物质干扰的甲醛残留量测定法。采用乙酰丙酮分光光度法,利用“本底扣除”的方式,在410nm波长处对疫苗中甲醛的残留量进行测定。结果,酚红、中性红等有色物质均不会对测定结果产生干扰;在0.002~0.2mg/ml的范围内,吸光度与浓度呈良好的线性关系(r=0.9999,n=7);在0.005mg/ml、0.05mg/ml和0.15mg/ml三种添加浓度下,该方法的平均回收率分别为108.6%、97.5%和101.3%;对24批含有有色物质的疫苗进行测定,所得结果与专属性较强的HPLC法一致。该方法专属性强、准确度好、适用范围宽,可以作为疫苗中甲醛残留量的检测方法。     

GC-MS-SIM法测定水发食品中痕量甲醛

由于甲醛(Formaldehyde)严重危害人体健康，因此国内外均禁止将甲醛用于食品的加工处理。甲醛属细胞原浆毒，对人的神经系统、肺、肝脏均有损害，还是可疑致癌物。目前国内尚无食品中甲醛测定的国标方法。较为流行检测方法主要有分光光度法、气相色谱法、液相色谱法和示波极谱法等，但上述测定方法分别存在准确度、灵敏度不高，或选择性低，     

分光光度法测定纺织品中甲醛的不确定度评价

根据GB/T 2912.1-2009标准,采用乙酰丙酮分光光度法测定纺织品中游离和水解的甲醛。本文通过对不确定度分量的5个主要来源进行分析,对测量结果的不确定度进行了评价。标准物质和最小二乘法拟合标准曲线时所产生的不确定度对总的不确定度贡献较大。     

甲醛液可使牛羊催膘

甲醛液可使牛羊催膘５０ｋｇ水加入浓度为４０％的甲醛液１０－２０ｍｌ，配成０．０１％—０．０２％甲醛液，让牛羊自由饮服。但甲醛稀释浓度不要超过万分之二，否则，会引起牛羊中毒。本刊辑甲醛液可使牛羊催膘...     

鸡舍熏蒸消毒应注意事项

熏蒸消毒法是利用福尔马林（含40％甲醛的溶液）与高锰酸钾发生化学反应,快速地释放出甲醛气体,经过一定时间杀死病原微生物的方法。它是鸡舍消毒常用而且非常有效的一种方法。但也有一些养鸡户（场）因消毒操作方法不当而引发火灾等一些麻烦,给养鸡户（场）造成较大的经济损失。现将熏蒸消毒方法及注意事项介绍如下,供广大养鸡户参考。     

鹅种蛋孵化技术

种蛋消毒：采集的种蛋，应尽快送往蛋库进行消毒。通常用甲醛和高锰酸钾熏蒸消毒。每立方米空间用高锰酸钾15克，40％的甲醛30毫升，熏蒸20～30分钟。要求消毒的空间密闭，温度25～27℃，相对湿度75％～80％，消毒效果最好。使用甲醛、高锰酸钾熏蒸消毒时，应注意下列几点：     

减蛋综合症病毒的灭活试验研究

采用不同温度、浓度的甲醛处理病毒，在一定的条件下完全能够使病毒灭活而保持其红细胞凝集活性的特性。人而简易的灭活方法是加热至６５或７０℃，但是红细胞凝集活怀水平下降９７％，抗原材料损耗太多。较为理想的灭活方法是２０℃条件下，终浓度是０．１％的甲醛处理４８小时，不仅能使病毒完全灭活，而且病毒的红细胞凝集活性下降不超过５％。     

衍生气相色谱法测定鸡疫苗中游离甲醛的含量

建立了鸡疫苗中游离甲醛含量测定的衍生气相色谱法。用2,4-二硝基苯肼衍生鸡疫苗中的游离甲醛后,环己烷萃取衍生物,HP-5毛细管色谱柱分离,气相色谱分析,电子捕获检测器检测,外标法定量。游离甲醛质量浓度在0.05~5 mg/L内与峰面积呈良好的线性关系,回归方程为Y=2781.9X+14.525(r=0.9999)。平均加样回收率为99.44%(RSD=1.87%)。本方法准确稳定,灵敏度高,重复性好,可用于鸡疫苗中游离甲醛的含量测定。     

掺甲醛牛乳快速检测试纸的研制

为解决在牛乳收购环节快速检测出掺甲醛牛乳的方法,采用试管法和试纸法对牛乳掺甲醛进行检测比较,确定了试纸检测牛乳中掺甲醛的方法可行.探索了采用品红试剂制作的试纸方法,筛选出品红溶液和硫酸溶液最佳浓度,两种溶液按3:1比例加载到WFB-试纸上,自然干燥.用掺有不同浓度甲醛的牛乳测试试纸的灵敏度,检测结果表明,最低检出量为0...     

Efficiency of phenoxyethanol at removing formaldehyde from immersion fixed muscle tissue

The propensities of several fluids for extracting formaldehyde from fixed muscle tissue were compared. Following formalin fixation, similar samples of muscle tissue were immersed in solutions of 0.5%, 1%, 2%, 4% phenoxyethanol, 40% ethanol, 25% ethylene glycol or water. Following storage periods of 4, 25, 50 or 100 days, the formaldehyde content of the fluids was determined by sodium sulfite titration. The solution containing 40% ethanol was clearly superior to the others. Solutions containing 2% or 4% phenoxyethanol were least effective and there was a significant inverse linear relationship between the concentration of phenoxyethanol and amount of formaldehyde extracted. This suggests that phenoxyethanol decreases the ability of water to extract formaldehyde from fixed tissues.     

Transendoscopic Chemical Ablation of Progressive Ethmoidal Hematomas in Standing Horses

Objective —To examine the response of horses with progressive ethmoidal hematoma (PEH) to intralesional injection of 4% formaldehyde solution.
Study Design —Nasal passages of horses affected with PEH were examined endoscopically at different intervals to determine the effects of intralesional injection of formaldehyde solution.
Animals —21 horses with PEH.
Methods —PEHs were injected transendoscopically with 4% formaldehyde solution. Horses were examined endoscopically and retreated at different intervals until the PEH was eliminated or was so small that reinjection was not possible.
Results —Lesions diminished significantly in size or were eliminated after 1 to 18 injections (median, 5; mean, 7.0 ± 5.62). Seventeen lesions (60.7%) resolved completely after 1 to 18 injections (median, 5; mean, 7.2 ± 5.71). Five lesions decreased markedly in size but did not resolve after receiving 1 to 18 injections (median, 5; mean, 7.6 ± 6.66). Injection of these lesions was discontinued 4.0 to 25.1 months (median, 9.5; mean, 11.02 ± 8.446) after the first injection. The PEH of one horse was removed surgically after one injection. Three horses, one with bilateral PEH, were lost to follow-up. One horse developed signs of laminitis. No other complications were observed.
Conclusions —Horses with a PEH can be treated effectively by transendoscopic, intralesional injection of 4% formaldehyde solution.     

Disinfection for dermatomycoses in veterinary practice

Sporulating cultures of two strains of Trichophyton equinum, two strains of Trich. mentagrophytes and one strain of Microsporum canis were tested by a simple dipping technique for their sensitivity to ten currently used disinfectants. Antifungal effects on all cultures of dermatophytes were observed already after one-minute treatment with 0.5%-4% peracetic acid, 4% formaldehyde solution and concentrated solution of Lastanox super. The above strains were inactivated after five-minute treatment with 1% and 2% formaldehyde solution, 20% Iodonal B solution and Ajatin solution with 10% active substance. Fungistatic effects of various intensity were observed in the other disinfectants.     

具有除甲醛功能的真丝织物的制备及性能测试

利用天然藏青果提取物中的多酚成分能与甲醛发生化学反应的原理,制备具有除甲醛功能的真丝织物。采用正交试验优化制备工艺条件为:整理温度70℃,整理时间2.0 h,整理液pH 4。在此工艺条件下,用质量分数为8%的藏青果提取物粉末整理制备的真丝织物24 h对空气中甲醛的去除率可达到84.5%,较普通真丝织物对空气中甲醛的去除率提高74.7个百分点。进一步用金属离子处理真丝织物,可有效改善织物除甲醛功效的耐洗性能,其中:Fe2+离子处理织物经10次洗涤后,甲醛去除率仅下降5.2个百分点;Al3+离子处理后,甲醛去除率仅下降4.9个百分点。试验表明,利用藏青果提取物整理及金属离子后处理制备的真丝织物具有明显的除甲醛功效,且织物的耐洗性能良好,是一种新型健康环保的织物。     

建立密植高产桑园的桑压条苗甲醛处理法

为了改造低产桑园、建立密植高产桑园,研究建立了提高压条苗生产效率的压条苗甲醛处理法,研究结果表明压条苗甲醛处理的生根数比环割处理增加130%,比非环割增加190%;压条苗甲醛处理的新根的生长比环割处理高1.5倍,比非环割处理高1.3倍;成苗率也是压条苗甲醛处理最好。该方法是一种高效成苗的方法。     

婴幼儿配方乳粉中甲醛含量测定及暴露污染评估

采用气相色谱定量及气相色谱-质谱确证,建立婴幼儿配方乳粉中甲醛含量的测定方法,并对网购45份婴幼儿配方乳粉中甲醛的污染水平进行调查分析与暴露风险评估.通过优化前处理条件,考察方法的线性关系、精密度、准确度、检出限及定量限等指标,采用外标法定量.结果表明:该方法在0～10μg范围内线性良好(r>0.999),方法检出限为...     

Effects of intravenous administration of formaldehyde on platelet and coagulation variables in healthy horses

OBJECTIVES: To assess safety and determine effects of IV administration of formaldehyde on hemostatic variables in healthy horses. ANIMALS: 7 healthy adult horses. PROCEDURE: Clinical signs and results of CBC, serum biochemical analyses, and coagulation testing including template bleeding time (TBT) and activated clotting time (ACT) were compared in horses given a dose of 0.37% formaldehyde or lactated Ringer's solution (LRS), IV, in a 2-way crossover design. In a subsequent experiment, horses received an infusion of 0.74% formaldehyde or LRS. In another experiment, horses were treated with aspirin to impair platelet responses prior to infusion of formaldehyde or LRS. RESULTS: Significant differences were not detected in any variable measured between horses when given formaldehyde or any other treatment. Infusion of higher doses of formaldehyde resulted in adverse effects including muscle fasciculations, tachycardia, tachypnea, serous ocular and nasal discharge, agitation, and restlessness. CONCLUSIONS AND CLINICAL RELEVANCE: Intravenous infusion of formaldehyde at doses that do not induce adverse reactions did not have a detectable effect on measured hemostatic variables in healthy horses.     

A new treatment for trichophytosis of cattle]

A new way to treat ringworm in calves and young cattle giving 1 ml formaldehyde solution (10%) per kg BW intravenously is described.