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牡丹繁育栽培及催花技术 总被引:1,自引:1,他引:0
牡丹的繁殖可通过有性繁殖和无性繁殖,有性繁殖也称种子繁殖,无性繁殖有分株、嫁接、组织培养等。总结了牡丹繁育栽培技术,并介绍了牡丹催花技术,打破了传统的催花方法,使催花技术有了新的进展。 相似文献
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牡丹的繁殖可通过有性繁殖和无性繁殖,有性繁殖也称种子繁殖,无性繁殖有分株、嫁接、组织培养等。总结了牡丹繁育栽培技术,并介绍了牡丹催花技术,打破了传统的催花方法,使催花技术有了新的进展。 相似文献
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橡胶树热研8—79原生质体培养再生植株 总被引:1,自引:0,他引:1
【目的】优化橡胶树热研8-79原生质体分离和培养条件,建立橡胶树原生质体培养植株高效再生体系,为橡胶树体细胞杂交育种奠定基础。【方法】以橡胶树热研8-79花药愈伤组织建立的胚性细胞悬浮系为试验材料,设计不同酶组合、酶解时间和悬浮细胞继代时间进行原生质体分离,采用不同培养基、看护细胞浓度和接种密度进行原生质体培养,对原生质体分裂发育的小愈伤组织进行体细胞胚诱导及植株再生培养,统计原生质体的产量、分裂频率、体胚数及体胚萌发率。【结果】酶组合为纤维素酶1.50%+果胶酶0.15%+离析酶0.50%、酶解时间为12 h时,继代培养第5 d的胚性悬浮细胞达最高产量(3.6 ×107个/mL PCV);用酶解细胞和看护细胞继代培养基分别作为原生质体液体培养基和看护培养基,比使用KPR培养基更有利于原生质体的分裂,当看护细胞浓度为5%、接种密度为5 ×105个/mL时原生质体的分裂频率最高,达54%。原生质体持续分裂形成肉眼可见的小愈伤组织增殖后经体胚发生途径发育成完整的小植株。【结论】通过优化橡胶树热研8-79胚性悬浮细胞原生质体分离的酶组合、酶解时间、继代培养时间及看护培养过程中培养基组成、看护细胞浓度和接种密度等影响因素,可以建立橡胶树原生质体培养植株高效再生体系。 相似文献
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《农业科学学报》2016,(5)
The influence of grafting on agronomical and qualitative characteristics of four Sicilian eggplant landraces was investigated. Grafted and ungrafted plants were compared in the open field in the northern coast of Sicily. Solanum torvum seedlings were used as rootstock. Regardless of genotypes tested, grafting significantly increased total fruit production, marketable production, and number of marketable fruits, but did not affect weight of marketable fruits and waste production. Landrace 2(Sciacca), with black epidermal tissue and pyriform fruit shape, when grafted onto S. torvum not only gave a higher yield performance than ungrafted plants, but also showed a higher phenolic antioxidant content. Landrace 4(Sicilia), with black epidermal tissue and small cylindrical fruits also benefited, when grafted onto S. torvum, from a substantial increase in antioxidant fruit content. As consumers' demand for fruits and vegetables rich in compounds important for human health is steadily increasing, these landrace/rootstock combinations should deserve more attention by plant nurseries involved in grafted seedling production and interested in the valorization and conservation of eggplant biodiversity. 相似文献
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Histological Study on Soybean Somatic Embryogenesis 总被引:4,自引:0,他引:4
ZHAO Gui-lan YANG Xiang-dong GUO Dong-quan HU Zan-min. Biotechnology Research Center Jilin Academy of Agricultural Sciences Changchun 《(《农业科学与技术》)编辑部》2008,(2)
Soybean somatic cell could induce the development of embryoid which was similar to embryo morphologically and structurally. Somatic embryogenesis system of soybean was used to conduct genetic transformation of soybean because of its several advantages such as higher transformational efficiency,beetter synchronism and fewer plant chimeras among transgenic plants. After infected with agrobacterium tumefaciens,the initiation,differentiation and development of young cotyledon embryogenic cell of soybean which was cultured on selective culture medium with kanamycin were investigated through histological study. The result showed that somatic embryo was differentiated in non-bud differentiation way. The embryogenic cells were differentiated from epidermis of explant or cells in 1 layer or 2 layers,with the division of embryogenic cells and degradation and disorganization of surrounding cells,the embryogenic cells would form embryoid with analogous suspensor structure.Later,globular embryoid would extrude from epidermis then developed into heart-shape embryo.The experiment was expected to provide theoretical reference for the construction of high transformational system of using plant somatic embryogenesis induced by young cotyledon of soybean. 相似文献
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《(《农业科学与技术》)编辑部》2008,(2)
Soybean somatic cell could induce the development of embryoid which was similar to embryo morphologically and structurally. Somatic embryogenesis system of soybean was used to conduct genetic transformation of soybean because of its several advantages such as higher transformational efficiency,beetter synchronism and fewer plant chimeras among transgenic plants. After infected with agrobacterium tumefaciens,the initiation,differentiation and development of young cotyledon embryogenic cell of soybean which was cultured on selective culture medium with kanamycin were investigated through histological study. The result showed that somatic embryo was differentiated in non-bud differentiation way. The embryogenic cells were differentiated from epidermis of explant or cells in 1 layer or 2 layers,with the division of embryogenic cells and degradation and disorganization of surrounding cells,the embryogenic cells would form embryoid with analogous suspensor structure.Later,globular embryoid would extrude from epidermis then developed into heart-shape embryo.The experiment was expected to provide theoretical reference for the construction of high transformational system of using plant somatic embryogenesis induced by young cotyledon of soybean. 相似文献
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植物嫁接砧穗愈合是指同种或异种植物的细胞、组织或器官互相影响与作用,结合成一个完整有机体的过程,受砧穗自身和外界等多种因素的调节和控制,是植物学领域的研究热点。嫁接可使植物的产量及品质得到提升,并有助于植物更好地适应环境。了解植物嫁接砧穗愈合机制对提高嫁接成活率和嫁接技术在生产中的应用具有重要的意义。就当前植物嫁接砧穗愈合的形态学进程、生理生化机制、分子调控及影响因素等方面的研究进展进行了综述,并对未来研究方向进行了展望。 相似文献
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用两性花毛葡萄改良新品种"桂葡1号"(NW 196)作嫁接换种接穗,对野生毛葡萄雌花植株(砧木)进行高位嫁接试验。结果表明:毛葡萄嫁接后萌芽率高、成活率低,其中冬季硬枝嫁接成活率为0,夏季绿枝嫁接成活率为20.1%,越冬后成活率仅为1.2%。生产上采用硬枝或绿枝嫁接方法对野生毛葡萄成年植株进行换种改造的技术方案不可行。 相似文献
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In multicellular plants, development starts with an asymmetric division of the zygote into two differentiated cells. The nature and distribution of fate-determining factors operating during embryogenesis remain largely obscure. Laser microsurgery was used here to dissect two-celled embryos of the alga Fucus spiralis. Removal of protoplasts from the cell wall induced dedifferentiation. However, isolated cells within the walls followed their restricted fate. Moreover, contact of one cell type with the isolated cell wall of the other cell type caused its fate to be switched. The cell wall thus appears to maintain the differentiated state and to direct cell fate in plant development. 相似文献