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为探明化感物质香豆素的化感抑草作用机理,采用实验室培养皿生物监测法研究香豆素对多花黑麦草种子萌发及幼苗生长过程中主要生长和生理生化指标的影响,观察了种子和幼苗茎秆的超微组织结构变化。结果表明,与对照相比,香豆素显著降低了多花黑麦草种子的发芽势、根长、根干重、茎长和茎叶干重等生长指标(P<0.05);香豆素处理显著降低了吲哚乙酸氧化酶(IAAO)活性和叶绿素含量(P<0.05),同时显著提高了可溶性糖含量(P<0.05),以及4和6 d种子的蛋白质浓度(P<0.05);且在前期促进了多花黑麦草幼苗的过氧化氢酶(CAT)活性、抑制了过氧化物酶(POD)活性,而后CAT降低,POD先升高后降低(P<0.05),淀粉含量升高。100 μg/mL的香豆素水溶液只能在多花黑麦草种子发芽和幼苗生长初期可以很大限度的起到抑制作用,但不足以使其致死。香豆素抑制种子胚乳养分的分解与供应,破坏胚乳细胞的细胞膜、细胞壁,同时可能使细胞失水而导致质壁分离。 相似文献
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黄连解毒汤细粉及超细微粉组织特征扫描电镜观察 总被引:1,自引:0,他引:1
目的:比较黄连解毒汤中药细粉和超细微粉形态学上的差异。方法:黄连解毒汤中药分别进行普通粉碎和超微粉碎,利用扫描电镜对两种不同粉碎方式得到的粉末进行观察。结果:超细微粉碎颗粒大小均匀,电镜下观察基本无完整细胞存在。结论:黄连解毒汤经超微粉碎后,粉末颗粒细微均匀,复方均质度明显改善,绝大多数细胞破壁。 相似文献
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Geraldus Gunawan Golda Rani Saragih Yusuf Umardani Srikanth Karnati Hevi Wihadmadyatami Dwi Liliek Kusindarta 《Anatomia, histologia, embryologia》2020,49(2):173-183
This study was carried on the tongues of ten normal, healthy and adult fruit bats (Rousettus amplexicaudatus, also known as the nyap biasa bat) in Yogyakarta, Java Island, Indonesia. The tongue was protrusible, elongated and flat with a rounded apex, and its width and thickness increased gradually towards to lingual root. There were two main types of lingual papillae, mechanical (filiform) and gustatory (fungiform and circumvallate). The tongue was divided into three parts (apex, corpus and radix), and then, each part was subdivided into three regions (two lateral regions and a median region). There were six subtypes of the filiform papillae—three types on the anterior part (small, scale-like and giant), one type on the middle part (leaf-like papillae) and two types on the posterior part (rosette-shaped filiform and conical filiform papillae)—in addition to transitional papillae presented on the corpus and radix. Two types of gustatory papillae were represented by a small number of fungiform papillae that are scattered among the filiform papillae on the lingual apex and corpus, while three circumvallate papillae on the posterior part are arranged in a “V” shape pointing directly at the larynx. 相似文献
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Gian Gartiwa Ulfah Damia Emilia Ika Megawati Stanislaus I. D. Pradipta Geraldus Gunawan Srikanth Karnati Hevi Wihadmadyatami Dwi Liliek Kusindarta 《Anatomia, histologia, embryologia》2021,50(5):801-811
The Horsfield's treeshrew (Tupaia javanica) is one of the shrews found on the island of Java that mainly feeds on fruits and occasionally insects. This shrew has an important role in the ecosystem as it spreads plant seeds and controls insects. To this day, there is no available knowledge about the structure of Horsfield's treeshrew's tongue. Therefore, the aim of this study was to provide data on the tongue structure of the Horsfield's treeshrew through two different methods. We investigated the tongue morphology and lingual papillae distribution of Tupaia javanica using 1) scanning electron microscopy (SEM) for microscopic structure and 2) histology for microscopic organization. Our macroscopic results revealed that the tongue of Tupaia javanica can be divided into three parts: apex, corpus and radix. The dorsal surface of the medial corpus is the median groove extending to the radix. Observation using SEM and light microscopy (LM) showed that the apex of the tongue bears three types of papillae: scale-like filiform, small filiform and fungiform. These are rosette filiform papillae, cornflower filiform papillae, conical papillae and fungiform papillae in the corpus. Meanwhile, the radix contains small filiform papillae, scale-like filiform papillae, vallate papillae, conical papillae and fungiform papillae. On the lateral posterior radix, Weber's gland reacts positively to PAS reaction. Taking together, our research shows that there are variations in the papillae on the tongue surface of Horsfield's treeshrew, and the variations of these papillae are very dependent on the pattern, type of food and the function of the tongue. 相似文献
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The total number, distribution and size of the optic axons of the buffalo (Bos bubalis) were studied in five optic nerves using light and transmission electron microscopes. Semithin sections were cut at 0.40 μm with an ultramicrotome and stained with 0.3% toluidine blue for light microscopic analysis. The ultrathin sections were cut at 70 nm, mounted on copper mesh grids (No. 200) and stained with saturated solution of uranyl acetate dihydrate and examined with the transmission electron microscope. The optic axons were assessed in 10 regions of roughly equal area. The total number of optic fibers was estimated on semithin and ultrathin sections. The measurements of the axonal diameters were made on electron micrographs taken from various regions across the optic nerve. The mean value of the total number of optic axons calculated was 1 397 099 ± 10 995 and 1 584 255 by light and electron microscopes, respectively. There was very little difference in the density of the optic fibers between the 10 regions of the optic nerve. The optic nerve area ranged from 18.2 to 22.4 mm2 (mean, 21.3 mm2). The diameters of the optic axons ranged from 0.5 to 12.3 μm (mean, 3.3 μm). 相似文献
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目的 是通过扫描电镜(SEM)观察勐腊并殖吸虫成虫体棘、口、腹吸盘.为鉴别其虫种提供资料。方法为直接从人工感染的猫体内解剖取出勐腊并殖吸虫成虫,用2.5%戊二醛和1%锇酸固定后,乙醇脱水,离子溅射仪镀金,JSM-820扫描电镜观察。结果表明云南西双版纳瑶瓦区阿括拿青(阿括拿青)虫体的体棘均为丛生型皮棘。新回宽寨老熊青(老熊青)虫体体棘在腹吸盘上方以单棘为主,偶见双棘。腹吸盘下方,为单双棘混合存在。结论认为从SEM形态观察发现勐腊两个不同点采集的勐腊并殖吸虫形态上不完全相同,存在一定的差异。 相似文献
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应用扫描电镜观察了 5头奶牛产后不同时期子宫内膜的变化。结果发现 ,分娩后早期 ,奶牛子宫内膜部分破损 ,与破损部位相邻的细胞在产后 1周内相继脱落 ;未破损内膜上皮细胞表面的纤毛和微绒毛形状不整、数量减少。产后2周 ,基底细胞开始生长 ,至产后 4 5 d时生长完成 ;产后 4周 ,内膜破损区域缩小 ,上皮细胞表面纤毛和微绒毛形状规则、数量增加 ;产后 4 5 d,偶见上皮细胞缺失 ;至产后 6 0 d,子宫内膜上皮细胞完整、排列有序、被覆纤毛和微绒毛。上述结果表明 ,奶牛子宫内膜在分娩后 6 0 d内完成修复。 相似文献
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三种盐生境植物叶表的扫描电镜观察(简报) 总被引:9,自引:0,他引:9
用Hitachi S430扫描电镜观察温室培养的小花碱茅,野大麦和碱蓬幼苗在NaCl胁迫下的叶表结构,结果表明,碱茅叶表腺毛稀疏,腊被密度中等,气孔下陷,随盐浓度增加,腺毛数显著增加,野大麦叶表腺毛数为132 ̄196个/mm^2,划被略疏于碱茅,腺毛数随盐浓度增加的变化不大,但形状有所改变,气孔亦下陷。碱蓬叶表有浓密的腊被,气孔数达236个/mm^2,说明植物在盐渍环境下有相应的调整外部结构的适应 相似文献
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犬冠状病毒在犬体内分布的初步研究 总被引:1,自引:0,他引:1
对电镜检查粪便中发现有犬冠状病毒的 4只犬 ,进行了血常规、尿常规、肝肾功能和心电图检查 ,均未发现明显异常。扑杀后经电镜负染检查 ,除肠内容物外 ,在其肝、心、脾、肾和肺等实质脏器中均发现冠状病毒粒子 相似文献
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青海省四种草原毛虫卵壳表面特征的扫描电镜观察 总被引:1,自引:0,他引:1
利用扫描电镜观察了青海省鳞翅目昆虫──青海草原毛虫(Qnaephoraqinghaiensis),门源草原毛虫(G.menyuanensis),曲麻莱草原毛虫(C.qumalaiensis),久治草原毛虫(G.jiuzhiensis)的卵壳结构特征,并比较了卵壳的受精孔区和气孔等细微结构特征,以作为不同草原毛虫卵壳分类鉴定的参考。 相似文献
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本文对采自兰马鸡、黄鹂、金雕(小型卵、大型卵)、白鹇体上5种羽虱卵的亚微结构进行了扫描电镜观察。结果证实:低倍镜下卵壳表面较光滑;300倍以上观察时,不同种类结构各异,有呈斑块状、鳞片状凹花纹、瘤状突等;卵盖的形状亦因种而异,呈帽状或圆锥状;有的种类卵体周缘有锚钩形长丝状物等。这些形态结构可作为种间分类特征之一。 相似文献
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使用扫描电镜观察了贝氏隐孢子虫(Cryptosporidium baileyi) 在丝毛乌骨鸡体内内生发育虫体的三维形态。大量球形虫体嵌于微绒毛丛中。滋养体呈球形,直径1.54~1.76 μm 。裂殖体呈桔瓣形或旋涡形排列,直径2.4~3.0 μm ,内含8 个香蕉形裂殖子,大小为4 μm ×0.65 μm 。成熟大配子体直径为3.5 μm 。带虫空泡内、外侧基部均可观察到梳状结构,根据带虫空泡的结构将其分为2 类,其中一类为裂殖子或小配子的带虫空泡,另一类为卵囊的带虫空泡。 相似文献
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贝氏隐孢子虫内生发育虫体的扫描电镜观察 总被引:9,自引:1,他引:9
贝氏隐孢子虫内生发育各阶段虫体寄生于气管和法氏囊的微绒毛丛中 ,鸭对贝氏隐孢子虫的易感性低于鸡和丝毛乌骨鸡。滋养体呈球形 ,平均直径 1 3μm ;typeⅠ裂殖体 ,平均直径 2 4μm~ 3 0 μm ,内含 8个裂殖子 ,呈逆时针旋涡状或呈桔瓣状排列 ;typeⅡ裂殖体内含 4个裂殖子 ,两种类型裂殖体的裂殖子之间填充着颗粒状残体并包围着一个大的球形残体。成熟裂殖子逸出的方式分为 4种类型。裂殖子大小为 3 0× 0 6 μm ,呈香蕉形。大配子体呈球形直径 3 3μm~ 4 2 μm ,小配子体直径为 3μm~ 3 6 μm。小配子呈子弹状 ,大小为 1 2 μm× 0 3μm ,并观察到成熟小配子的释放方式。带虫空泡可分为无球形残体和有球形残体两类 ,根据观察结果推测带虫空泡基部的梳状结构不具备营养器官的功能 ,营养器官应为带虫空泡的底部 相似文献
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应用扫描电镜对卫星搭载的2个紫花苜蓿(Medicago sativa L.)品种的花粉显微结构进行了观察研究.结果表明:花粉为长球形和近球形2种,赤道面观为椭圆形,极面观为三裂圆形,具3(拟)孔沟,沟为条形,狭缝状,沟长至两极,孔处有外突或无;花粉外壁纹饰为穴状、粗糙状、网状3种;花粉的外壁纹饰不同品种间以及同一品种的不同处理间均有差异.这2个紫花苜蓿品种的花粉形状、大小和外壁纹饰与地面对照对应品种的花粉存在显著差异(P<0.05),表明空间飞行因子可导致花粉发育异常及可遗传突变等诱变效应.卫星搭载的2个品种存在花粉败育,不同品种间败育花粉数量存在差异.败育花粉粒形状干瘪,外形异常. 相似文献
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通过病理剖检、病理组织学及电镜负染观察等方法对来自山东、河南等地的15只表现典型新城疫症状的发病鸵鸟的死亡原因进行系统研究分析。病理剖检可见发病鸵鸟全身广泛性出血,其中以消化道黏膜出血为主;病理组织学观察可见其病理特征以消化道黏膜出血,细胞变性、坏死、脱落为主,同时伴发心肌纤维和肾小管上皮细胞的颗粒变性,脾脏和肺脏充血、出血以及其头颈部的水肿,淋巴组织的坏死等病理变化。电镜负染观察可见圆形、椭圆形或纺锤形,直径200~500 nm,具有明显纤突的典型副黏病毒粒子。以上试验结果证实鸵鸟的死亡原因为新城疫病毒感染所致。 相似文献
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Shoko NAKAMUTA Makoto YOKOSUKA Kazumi TANIGUCHI Yoshio YAMAMOTO Nobuaki NAKAMUTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):245-250
In turtles, the epithelia lining the upper and lower chambers of the nasal cavity project axons to theventral and dorsal parts of the olfactory bulbs, respectively. In a semi-aquatic soft-shelled turtle,Pelodiscus sinensis, more than 1,000 odorant receptor genes have been found, but it is notknown where they are expressed. In this study, we aimed to clarify the distribution of cells expressing thesegenes in the olfactory organs of soft-shelled turtles. Immunoreactions for the Gαolf, the α subunit of Gprotein coupled to the odorant receptors, were detected on the surface of epithelia lining both the upper andlower chambers of the nasal cavity. The receptor cells in the epithelium of both chambers possessed cilia onthe tip of their dendrites, whereas microvillous, non-ciliated, receptor cells were not found. These datasuggest that the odorant receptor genes are expressed by the ciliated receptor cells in the upper and lowerchamber epithelia. Precise location of the vomeronasal epithelium is not known at present. 相似文献
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青海草原毛虫(Gynaephora qinghaiensis)是高山草甸上的重大害虫之一,雄成虫具有飞行能力能够扩散危害.本研究利用扫描电子显微镜观察青海草原毛虫雌雄成虫翅的感器种类和形态结构,旨在明确青海草原毛虫雌雄成虫翅的感器种类及分布特征并推测各感器的功能.结果表明:青海草原毛虫雌雄成虫翅共发现7种感器,分别是毛形感器(Ⅰ型、Ⅱ型、Ⅲ型和Ⅳ型)、刺形感器(Ⅰ型和Ⅱ型)、舌形感器、叉形感器、锥形感器、指形感器、水滴形感器.其中,毛形感器数量最多,分布较广泛.本研究基于前人对其他昆虫各组织的感器研究,对青海草原毛虫雌雄成虫翅感器的超微结构进行分析并推测其功能,这将为青海草原毛虫的形态学、飞行能力及寻找防治靶标奠定基础. 相似文献