A stereological analysis on the calculation of the volume values of thoracic segments in ducks

Stereology is a method for examining two-dimensional objects as three-dimensional objects. The aim of this study was to offer volume values for thoracic segments in ducks by means of stereological methods. This study examined the data obtained from stereological analysis of the total volume and grey and white matter volume values of the thoracic segment, a part of the adult duck spinal cord with a weight of 3–4 kg. In the study, study samples consisted of 10 adult ducks (Anas) used without gender discrimination. To perfuse all of the animals, 10% formaldehyde was utilised. The perfused animals were kept in 10% formaldehyde for one week. The spinal cord was uncovered following the removal of arcus vertebrae parts of thoracic vertebrae in the thoracic part of dissected ducks. Tissue samples of thoracic segments were taken; 5-µm-thick cross sections from these tissue samples were taken via microtome. Attention was paid to obtaining samples at the ratio of 1/250 by taking 12 cross sections from each segment. The cross sections were subjected to haematoxylin-eosin staining. Photographs of all cross sections were taken using a microscope. The volume values of all tissue and grey and white matter structures in each thoracic segment of the spinal cord were calculated. The total volume, grey and white matter volume densities in thoracic segments of ducks, as well as the data obtained as a result of proportioning volume values of one another are represented in the conclusion section of this study.     

A Morphological and Stereological Study on Cervical Segment of Spinal Cord of Quails

In this study, volume densities of white and grey matters of cervical segments of spinal cords of quail were investigated stereologically. In both sexes, mature, six quails were used as material of this study. All animals were fixed by perfusing in 10% buffered formalin. Tissue specimens were obtained from cervical spinal cords. These tissue specimens were cut every fiftieth section at 5 μm thickness by a microtome. And mean six or seven sections were examined from every block by this method at microscope. After that, these sections were stained by haematoxylin eosin and photographed. Densities of volumes of all tissues of cervical segments of whole spinal cords and white and grey matters were calculated with principle of Cavalieri. As a result, total volume of spinal cord, volumes of white and grey matters of cervical segment and volume rates of white and grey matters were calculated.     

A morphometric and stereological study on cervical spinal cord segment of goose

In this study, volume density of white matter and grey matter areas of cervical segment of spinal cord in adult geese weighing 3–4 kg was examined using stereological methods. 10 geese were used as material without sex discrimination. All animals underwent perfusion with 10% buffered formaldehyde. Following the perfusion, animals were kept in 10% formaldehyde for 1 week. Geese were then dissected. Cervical area of spinal cord was revealed removing cervical spine. Tissue samples were obtained from each segment of cervical area. 5 μm thick cross‐sections were taken from these tissue samples via microtome. Series of cross‐sections were obtained by sampling in the ratio of 1/250 including 12 cross‐sections from each cervical segment of every animal. Cross‐sections were stained by haematoxylin eosin. They were photographed under microscope. Volume density (volume fractions) of both whole tissue and white matter and grey matter parts in each cervical segment of spinal cord were calculated using Cavalieri’s Principle. In the study, total volume of cervical segment, volume of white matter and grey matter, and ratios of these volumes one another were assessed in goose.     

A morphological and stereological study on brain,cerebral hemispheres and cerebellum of New Zealand rabbits

The aim of this research was to determine brain, cerebral hemispheres and cerebellum volume and volume ratios by using stereological methods and investigate morphological differences between female and male New Zealand rabbits. The study was applied on 14-month old (10 male and 10 female) New Zealand rabbits. The materials removed from the cavum cranii using dorsal approach. After following routine histological procedure, paraffin blocks were prepared and cut every seventieth section at 10 μm thickness. Slides were stained with Crossmon's triple stain and photographed. The sectional images obtained were transferred to ImageJ program to estimate grey and white matter volume on cerebral hemispheres and cerebellum with principle of Cavalieri. According to results, there was no asymmetry on the left and right cerebral hemispheres of New Zealand rabbits. In the total hemisphere volume calculated by Cavalieri principle, grey and white matter ratio was 81.57% and 18.43% in female, 82.80% and 17.20% in male. It was found that the white matter was significantly higher in females than males in cerebral hemispheres (p < .05). Also, it was found that grey and white matter ratio in total cerebellum volume was 67.82% and 32.18% in female, 67.94% and 32.06% in male respectively. It was determined that the females' white matter was larger than male rabbits in cerebellum (p < .05). It is thought that morphometric data obtained from this study will contribute to the existing anatomical knowledge and also considered as reference values in the clinical sciences.     

Effect of soaking,fermentation with lactobacillus and enzyme treatment on nitrogen‐corrected apparent metabolizable energy values of wheat in cockerels and quails

An experiment was carried out to determine nitrogen‐corrected apparent metabolizable energy (AMEn) values of processed wheat products with two levels of enzyme (0 and 0.5 g/kg) and five processing methods [control (C), water‐soaking for 12 h (SO12h), 24 h (SO24h) and 48 h (SO48h) and fermentation with lactobacillus (FL)] in two species of poultry (cockerel and Japanese quail). In this study, each processing method group had six replications with two cockerels or four quails per replication. The results showed that the calculated AMEn values of processed wheat by different methods were as follows: C‐12.61 and 12.60; SO12h‐12.75 and 12.73; SO24h‐12.80 and 12.79; SO48h‐12.97 and 12.96; and FL‐13.36 and 13.33 MJ/kg for cockerels and quails, respectively, indicating that fermentation improved AMEn values compared with the others. The mean AMEn of processed wheat with enzyme (13.12 MJ/kg) was significantly higher than that of processed wheat without enzyme (12.64 MJ/kg). However, there were no significant differences in the AMEn of processed wheat between cockerels and quails. The interaction between species and levels of enzyme in AMEn values was not significant. There was no significant difference (p > 0.05) in three‐way interactions among species, levels of enzyme and wheat processing methods for AMEn values. In conclusion, the results of the experiment suggest that the FL was more effective processing method of wheat to obtain high level of AMEn values than the others, and the combination of enzyme and processing methods can increase the AMEn values compared with processing methods alone.