Effects of inoculants and wilting on silage fermentation and nutritive characteristics of red clover–grass mixtures

Various management practices (e.g. wilting, application of silage additives or adding a grass component) can be used to improve silage fermentation of pure red clover (Trifolium pratense L.). Therefore, the aim of this laboratory ensiling study was to investigate the effects of varying proportions of red clover and perennial ryegrass (100/0, 66/33, 33/66, 0/100) on silage quality during two consecutive years. In addition, two wilting levels [target dry matter (DM): 300 vs. 400 g kg^?1] in combination with lactic acid bacteria (LAB) additives were tested. Herbage was ensiled, either untreated or inoculated with homofermentative LAB (low wilted) or homo‐ and heterofermentative LAB (high wilted). In most cases, lactic and acetic acid decreased as the proportions of ryegrass were increased. Data concerning ammonia‐N concentrations showed considerable differences between cuts and years. Silages treated with homofermentative LAB generally had high lactic acid and low final pH, whereas acetic acid and 1,2‐propanediol tended to be higher when homo‐ and heterofermentative LAB were applied. Inoculants had a positive effect on DM losses and ammonia‐N in only a few silages. Wilting decreased DM losses and fermentation acids at most cuts, irrespective of the grass/clover ratio in the herbage mixture. There was a strong year effect on the organic matter digestibility (DOM) of the silages. In conclusion, the optimal strategy for successful silage fermentation of red clover is the ensiling in mixtures with ryegrass. Furthermore, herbage should be wilted to a DM content of about 300–350 g kg^?1. The application of LAB inoculants did not alter the DOM but did improve silage fermentation.     

The effect of dry feed additives on the nutritive value of silage

Silages were prepared in late September from a mixed crop of perennial ryegrass and white clover with low dry matter and high nitrogen content. Six different treatments were used during ensiling; addition of either molassed sugar beet pulp or rolled barley, at 50 kg and 25 kg (t fresh grass)^?1, formic acid at 51 t^?1 and no additive (control). All silages were well fermented with low levels of ammonia and pH. The addition of rolled barley or sugar beet pulp increased the dry matter content of the silages incrementally and appeared to assist the retention of nitrogen in the silage. Dry matter intakes of silages with 50 kg of barley or sugar beet pulp tonne^?1 were similar to the control silage when fed to wether sheep but at 25 kg t^?1, dry matter intake increased by 0·15 with barley and 0·04 with sugar beet pulp compared to the control. The digestibility of dry matter (DM) and organic matter (OM) were significantly higher than the control for silages treated with formic acid or high levels of barley and sugar beet pulp whereas neutral-detergent fibre (NDF) digestibility did not appear to be significantly affected. All treated silages had a significantly higher metabolizable energy (ME) content than the control, and additions of barley or sugar beet increased the ME content. The ME intakes of all treated silages were also significantly greater than those of the control, and the formic acid-treated and low barley silages were the highest. This appeared to be associated with significantly higher energy digestibility and DM intake. Addition of rolled barley during ensilage resulted in greater nitrogen intake, availability and retention compared to additions of sugar beet pulp. The higher retention with barley silages was associated with a significantly lower proportional loss of absorbed nitrogen in urine, which indicated a more efficient utilization of nitrogen. These differences require further study to determine the mechanisms involved in the interactions between the energy source and herbage protein, both during fermentation and in the rumen.     

LOSSES IN THE NUTRITIVE VALUE OF HEAVILY-WILTED HERBAGE ENSILED IN EVACUATED AND NON-EVACUATED POLYTHENE CONTAINERS

A conservation experiment is described in which the losses entailed in ensiling heavily-wilted herbage of 37–43% dry-matter content either by complete sealing in polythene alone, or by complete sealing followed by evacuation of air, were investigated.
The metabolizable energy values of the grass and silages were determined, and the silage volatile-acids fraction investigated by gas chromatography. The results indicate that there are no significant fermentation differences between the two types of silage. The mean dry-matter loss for the sealed, unevacuated silos was 6.5% and for the evacuated silos 7.8%. These losses, and the individual crude- and digestible-nutrient losses, are discussed in relation to other published work.     

The influence of the carbohydrate fraction and additives on silage quality

Respiration losses, effect of sugar content, formic acid addition and inoculation were evaluated in relation to initial fermentation and silage quality in three experiments. Changes in the content of fermentable substrate during post–harvest respiration and initial fermentation were studied in the laboratory. Soluble carbohydrates were estimated as glucose, fructose, sucrose, fructans and starch by an enzymatic method.
About 70% of the sugars remained after 30 h of conventional wilting, as opposed to less than 50% in a chopped sample kept in the dark. During the first days of fermentation the metabolic activity was very intensive. Sugars, which accounted for 10% of the dry matter, were fermented within 4 d. There were indications, mainly in clover silages, that substances other than water-soluble carbohydrates were used as energy sources.
Low-sugar crops were obtained by shading the fields. After 3 d the sugar in the grass had decreased to half of the original content. This effect was less pronounced in clover. The amount of available substrate and dry matter (DM) content had a major influence on silage quality. In an unwilted crop at least 25 g of water-soluble carbohydrate was needed per kg of fresh material to obtain an acceptable silage quality in the controls, while 20 g was sufficient in inoculated silage. Formic acid addition makes the fermentation less sensitive to water–soluble carbohydrate content. Inoculation of willed silage increased lactic acid production and decreased the pH. There was also less variation in silage quality.     

Silage and milk production: a comparison between barley and dried sugar-beet pulp as silage supplements

Two grass silages made from perennial ryegrass, and with D-values of 0·216 and 0·255, were offered ad libitum to 18 Ayrshire cows in two feeding experiments. On the control treatment the silages were supplemented with soybean meal only, and on the other two treatments with equal weights of DM from either barley or dried molassed sugar-beet pulp plus the same weight of soya as on the control treatment. The daily intakes of silage DM were not significantly different on the barley and beet-pulp treatments, and, on average, the intake of silage DM was reduced by 0·24 and 0·20 kg by feeding 1 kg barley and beet pulp DM respectively.
The daily milk yields were not significantly different on the barley and beet-pulp treatments with mean values of 19·2 and 19·2 kg per cow respectively compared with 17·2 kg on the control treatment. On the barley and beet-pulp treatments the fat, SNF, CP and lactose concentrations in the milk and the live weights of the cows were not significantly different. It is concluded that the barley and beet pulp had similar feeding values and replacement rates when used as supplements with grass silage, and that the two feeds were interchangeable on an equal DM basis.     

Effect of herbage water-soluble carbohydrate content and weather conditions at ensilage on the fermentation of grass silages made on commercial farms

A total of 1009 samples of silage made in bunker silos on commercial farms between 1972 and 1978 was analysed to investigate the effect of herbage water-soluble carbohydrate content (WSC) and weather conditions at ensilage on fermentation as measured by ammonia-N concentration and pH of first-cut grass silages.
Silage dry matter (DM) content had the major effect on fermentation. Factors influencing silage DM were rainfall and hours of sunshine during silage making, and DM content of the grass cut. WSC content of herbage ensiled also had a significant effect on subsequent fermentation. The major influences on herbage WSC were hours of sunshine and rainfall during the growing season.
The effect of chemical additives, albeit at poorly defined and often inadequate rates, was small in comparison to that of silage DM.
The minimum DM necessary to produce well-fermented silage without additive was approximately 260 g kg⁻¹. Use of formic acid significantly reduced this requirement to 240 g kg⁻¹ and to 252 g kg⁻¹ for sulphuric add + formalin. The results indicate that the minimum herbage WSC necessary to prevent a clostridial fermentation developing in silage with a DM content of 230 g kg⁻¹ is approximately 37 g kg⁻¹ without additive and 30 g kg⁻¹ with formic acid.
It is concluded that on commercial farms, weather conditions i.e. amount of rainfall and sunshine prior to and at ensilage, have a greater effect on subsequent silage fermentation than additive use.     

The microbiological and chemical composition of silage over the course of fermentation in round bales relative to that of silage made from unchopped and precision-chopped herbage in laboratory silos

The composition of baled silage frequently differs from that of comparable conventional silage. A factorial experiment was conducted with three wilting treatments (0, 24 or 48 h) × three ensiling systems [unchopped grass in bales, unchopped grass in laboratory silos (LS), precision-chopped grass in LS] × six stages of ensiling to (i) confirm that the fermentation of unchopped grass in LS could be used as an adequate model for baled silage fermentation, (ii) quantify the differences between baled silage and silage made from precision-chopped herbage across a range of dry-matter contents and (c) quantify the fermentation dynamics within the various treatments. The onset of fermentation as evidenced by the accumulation of fermentation products and the decline in pH were slower ( P  < 0·05) in baled silage compared with silage made from precision-chopped herbage. Furthermore the pH ( P  < 0·001) and overall concentration of fermentation acids ( P  < 0·01) were lower while ammonia-N concentration was generally higher in baled silage, making it more conducive to the activities of Clostridia , Enterobacteria and yeast. Numbers of Enterobacteria were higher ( P  < 0·001) in baled silage in the early stages of ensilage and persisted in baled silage at the end of the storage period. The implications of a slower onset of fermentation in baled herbage are greater in farm practice, as the fermentation would be further restricted by a more extensive wilting of the herbage prior to ensiling.     

The effects of incorporating sugar-beet pulp with herbage at ensiling on silage fermentation, effluent output and in-silo losses

Three studies were undertaken with the aim of examining in-silo losses associated with ensiling mixtures of beet pulp and herbage. In Experiment 1, first-regrowth perennial ryegrass was ensiled untreated or mixed with either 40 or 120 kg unmolassed beet pulp t^?1 fresh grass prior to ensiling. In each of Experiments 2 and 3, second-regrowth ryegrass was ensiled untreated or mixed with either 40, 80 or 120 kg unmolassed beet pulp t^?1 fresh grass prior to ensiling. Dry-matter concentrations of herbage ensiled in Experiments 1, 2 and 3 were 141, 157 and 139 g kg^?1 respectively. Time-course studies indicated a more rapid fermentation with the untreated silages. Ensiling beet pulp with herbage resulted in significant improvements in silage fermentation, reflected in reductions in silage pH and ammonia nitrogen concentrations, with these effects being pronounced at beet pulp inclusion in excess of 80kg t^?1. Beet pulp inclusion increased the dry-matter concentrations of the resulting silages while reducing crude protein, modified acid detergent fibre and gross energy concentrations. In Experiment 1 beet pulp retained 1·62 and 1·641 effluent kg^?1 at the 40 and 120kg t^?1 application rates respectively. In Experiments 2 and 3, effluent retentions were 1·40, 1·29 and 0·93 1 kg^?1 and 2·87, 2·19 and 1·781 kg^?1 at the 40, 80 and 120kg t^?1 beet pulp inclusion rates respectively. In each of Experiments 1 and 3, in-silo losses were reduced with increasing level of beet pulp inclusion, while in Experiment 2 in-silo losses were relatively unaffected by treatment. Nutrient losses in effluent were reduced by the inclusion of beet pulp in all three experiments, while nutrient losses in surface waste and invisible nutrient losses tended to increase with higher levels of beet pulp inclusion. It is concluded that the inclusion of beet pulp with herbage at ensiling can result in an improved silage fermentation and a reduction in in-silo losses, while at the same time retaining considerable quantities of effluent within the silo. However, with herbage dry-matter concentrations below 160gkg^?1, beet pulp inclusion in excess of 120 kg t^?1 herbage would be required to eliminate effluent production totally.     

Field‐related quality management system for grass silage production

A model for the prediction of the silage quality was tested in several large farms in northeast Germany. The model uses data and information on the fresh grass and the application of silage additives at ensiling to evaluate the “ensilability.” During clamp filling, the model analyses information on the filling performance, activity of compacting machines and hermitic covering of the clamp and evaluates the “ensiling technique.” Both “ensilability” and “ensiling technique” are assessed as “good,” “medium” or “bad” and pooled in “silage evaluation grades” 1–5. In the present model version, the predicted silage net energy lactation (NEL) and crude protein (CP) content is between 3% (for the best grade 1) and 15% (for the weakest grade 5) lower than the fresh grass NEL and CP content. In our experiments, all the grass silage production systems were characterized by “good” “ensilability” and “ensiling technique.” Under those circumstances, the experimental results indicated a closer numerical relation between fresh grass and measured silage NEL and CP content than between predicted silage and measured silage NEL and CP content. The results reveal that the model prediction calculation should be changed compared to the present version. If “ensilability” and “ensiling technique” are evaluated as “good,” the predicted silage NEL and CP content should be forecasted in a range between 0% and 3% decrease compared to fresh grass.     

Effects of applying a bacterial inoculant to silage immediately before feeding on silage intake, digestibility, degradability and rumen volatile fatty acid concentrations in growing beef cattle

In an experiment, involving twelve male cattle (initially 235 kg live weight), the effects of applying lactic acid bacteria [Lactobacillus plantarum; 10⁹ colony-forming units (g fresh silage)^?1] to grass silage, immediately prior to that silage being fed, on dry-matter (DM) intake of the silage, degradability of nitrogen (N) and fibre in the rumen, total tract digestibility and composition of rumen fluid in the animals were examined. A grass silage, which had been made from the primary growth of a predominantly perennial ryegrass sward, was offered as the sole diet. The inoculant was applied to the silage at the rate of 2 g of freeze-dried powder reconstituted in 12 ml of water (kg fresh silage)^?1 immediately prior to that silage being fed and an equivalent amount of water was applied to the silage in the control treatment. The two diets were compared in a change-over design. The silage was well preserved, having a pH and concentrations of ammonia N and butyrate of 3.72, 74 g (kg total N)^?1 and 0.11 g (kg DM)^?1 respectively. Application of the inoculant significantly increased true protein, acid-insoluble N and water-soluble carbohydrate concentrations (P < 0.001) in the diet. Silage DM intake was not significantly increased (P= 0.072) by this of inoculant treatment, which had no significant effect (P > 0.05) on rumen degradability or total tract digestibility of DM, N, neutral detergent fibre or modified acid detergent fibre. Rumen pH, ammonia concentration or the molar proportions of volatile fatty acids were not altered (P>0.05) by inoculant treatment. It is concluded that application of the inoculant to the silage prior to silage being fed did not significantly affect silage DM intake, total tract digestibility, or degradability or fermentation in the rumen of cattle offered grass silage as the sole diet. It is also concluded that the results of this experiment provide no evidence that the mode of action of L plantarum, applied as an additive to grass at ensiling in previous studies, is through ‘direct’ effects in the rumen.     

An evaluation of three bacterial inoculants and formic acid as additives for first harvest grass

An experiment was carried out during 1984 to study the effect of treating grass at ensiling with three commercially available inoculant-type additives (H/M Inoculant, Grass Sile and Siron), formic acid (850 g kg⁻¹; Add-F) or no additive on grass preservation, in-silo loss, intake and animal performance. Primary growth grass ensiled from 28–29 May into concrete-walled covered silos was of high dry matter (DM, 234 g kg⁻¹), water-soluble carbohydrate content (WSC. 212 g kg DM⁻¹) and digestibility (MADF, 250 g kg DM⁻¹).
The untreated silage displayed good preservation and with the exception of the Sirontreated silage which showed significantly lower buffering capacity (Be) and volatile fatty acid (VFA) contents than the untreated silage, the application of inoculant-type additives did not improve silage preservation or decrease in-silo DM losses. The formic acid-treated silage displayed significantly lower Be, water-soluble carbohydrate, ash, ammonia nitrogen (g kg total N⁻¹) and lactate contents than the untreated silage.
After a 133 d storage period, silages were offered to finishing beef cattle for an 84-d period. Cattle offered the silages displayed similar and non-significant daily DM intakes, daily liveweight gains, dressing proportions and daily carcass gains. From this experiment it appears unlikely that any of the additives evaluated will improve animal performance relative to a well-preserved untreated silage.     

THE GENERAL PATTERN IN SILAGE FERMENTATION IN TWO SUBTROPICAL GRASSES

Three sets of previously-published experiments have been re-analysed by numerical methods intended for the elucidation of pattern in sequential data. The results suggest that for Setaria sphacelata cv. Nandi and Chloris gayana cv. Samford Rhodes the stable terminal silage to be expected under subtropical conditions is not the high lactic-acid silage of temperate regions, but a silage with a relatively high proportion of acetic acid. Lactic-acid silage is formed, but usually behaves as though in a transitional phase. However, silage made from mature Setaria can be maintained in the lactic-acid phase by the addition of high percentages of sugar. The silage fermentation can sometimes be retarded, or deflected into an undesirable path for reasons that are not understood.
The above patterns suggest three hypotheses for more fundamental investigation. First, since acetic-acid silage can be expected under most subtropical conditions, its properties require careful investigation. Secondly, relatively young Setaria may be regarded as a source of acetic-acid silage, but the optimum amount of sugar to be added for ensiling more mature grass needs investigation. Thirdly there is an urgent need to investigate the causes of retardation of fermentation, and of its deflection into an undesirable path, because until these causes are understood, the outcome of ensilage will remain uncertain.     

A comparison of the ensilage, in an anaerobic cabinet, of grass, either fresh or when thawed after deep freezing

The suitability of freezing a large amount of grass from a single harvest for use in a series of experiments to investigate silage fermentation and its manipulation was examined. Perennial ryegrass, harvested in late June, was ensiled in sterile polythene bags inside an anaerobic cabinet; some was also stored frozen at -20°C for up to nine months before ensiling in a similar manner. The course of fermentation was followed using not only conventional chemical and microbiological analyses, but also by measuring microbial metabolic activity in vitro. Freezing and thawing affected the chemical composition and the microbial populations on the grass, and there were differences in the changes occurring in thawed grass during ensilage, probably related to damage to plant cells and impaired microbial activity associated with freezing and thawing. For both the grasses and the silages made from them, it was clear that the number of microorganisms present was not a reliable guide to microbial metabolic activity. Although the fermentation of frozen-and-thawed grass did not simulate precisely the fermentation occurring with fresh grass, it is concluded that, when used judiciously, the ensilage of thawed grass can be a useful experimental tool, particularly in the initial screening of manipulants of fermentation.     

The effect of wilting and silage additives on the fermentation of autumn made grass silage ensiled in bunkers on commercial farms in South Wales 1983-85

Silage making practices in respect of 130 samples of autumn made grass silages ensiled in bunkers on commercial farms in South Wales during 1983-1985 were analysed to discern the effect of wilting and/or silage additives on fermentation. Silages were primarily made during late September and early October in fine weather from perennial ryegrass pasture which had not been grazed for 6 weeks. On average 13.9 ha of pasture was cut for silage. Analysis of 120 samples of grass showed it contained 176 g kg^?1 dry matter (DM) with (g kg^?1 DM) 215 protein, 240 modified acid detergent fibre (MADF), 78 water-soluble carbohydrate (WSC) and 95 ash. Most farmers attempted to pre-wilt grass for 1 day prior to ensilage and one quarter of them made unwilted silage. Silage making was usually completed within 6 days of starting to cut and was generally made with a precision-chop machine. Silage additives applied were (kg t^?1) formic acid (4·7), formic acid 4-formalin (5·5), sulphuric acid-+ formalin (5·0) sugars (14) and inoculants (0·65). Formic acid significantly reduced pH, and formic acid with or without formalin significantly reduced ammonia nitrogen (N) content of silages compared with other treatments. Protein contents of acid/formalin treated silages were significantly higher and MADF of acid with or without formalin treated silages were significantly lower than other treatments. Pre-wilting grass prior to ensilage did not significantly increase subsequent silage DM content and significantly increased the pH of non-additive treated silages. Unwilted silages treated with formic acid with or without formalin had a significantly lower ammonia-N content and higher residual WSC than other treatments. It is suggested that only formic acid application either alone or in conjunction with formalin to unwilted silage was successful in producing well preserved silages and that a grass WSC content of 17 g kg^?1 would be necessary to achieve this.     

THE EFFECT OF A RYEGRASS COMPANION GRASS AND THE VARIETY OF RED CLOVER ON THE PRODUCTIVITY OF RED-CLOVER SWARDS

An experiment was conducted to measure the effects of differrat ryegrass companion grasses and red-clover varieties on the productivity of red-clover swards. Three silage harvests per year were taken over a 2-year period. The addition of a companion grass increased total herbage yields; S24 perennial ryegrass gave the highest herbage yield over the two years, followed by Reveille perennial ryegrass. Because of lack of persistence, Tetila Italian ryegrass yielded poorly the second year. A companion grass had little effect on red-clover yields in the mixed swards but improved percentage digestibility of the OM of the total herbage and lowered the CP percentage. Its presence also reduced the ingress of unsown species. The variety of red clover used had little effect on total herbage yields or red-clover yields in the first harvest year. In the second year, Hungaropoly and Tilo persisted better and so gave higher total herbage yields and red-clover yields than Dorset Marl or Essex. The fall in total herbage yields from the first to the second year was entirely due to a fall in red-clover yield since yields of the ‘non-red clover’ fraction of the total herbage increased. A red-clover/grass sward may have advantages over a pure red-clover sward nutritionally, for silage-making and for its effect in diluting the oestrogenic activity of a pure clover sward. A major reappraisal of the role and potential of red-clover swards in the UK is warranted because of their many valuable attributes, particularly their ability to give high herbage yields of high nutritive value without the addition of fertilizer N. The improved persistency of some of the tetraploid varieties of red clover enhances the value of the plant.     

Fresh and ensiled forage plants—total composition,silage losses and the prediction of silage composition from the crop

Twenty ley and whole‐crop samples were analysed before and after ensiling to determine the proportion of dry matter (DM) that could be accounted for by the sum of 12 chemical assays for ash, ash‐excluded‐amylase‐treated neutral detergent fibre (aNDFom), starch, water‐soluble carbohydrates (WSC), pectin, crude protein (CP), CP in aNDFom (CPndf), ammonia, crude fat, phenolics, plant organic acids and liquid fermentation products (acids and alcohols). Crop components, utilized during silage fermentation and the possibility of predicting silage composition from that of the crop, were also investigated. Samples consisted of timothy and red and white clover, harvested at early and late maturities in two cuts per maturity and of whole‐crop barley, wheat and maize, harvested at early, intermediate and late stages of maturity. Ley crops were wilted to reach a DM content of approximately 400 g/kg, whereas whole crops (WC) were not wilted (151–757 g DM/kg) before ensiling. The average sum of analytes was 1022 and 981 g/kg DM for crops and silages respectively. An overall closeness to complete recovery indicates that no major plant components were missing from the analyses. Relative proportions of pectin, plant organic acids and phenolics, which are rarely analysed, were approximately 60:40:2 (w/w). Ash, aNDFom, crude fat and CP were almost completely recovered after ensiling, whereas partial metabolism reduced recoveries of starch (81%), CPndf (62%), plant organic acids (65%), pectin (64%) and WSC (29%). Only the four analytes with high silage recoveries could be reasonably well predicted from parent crop levels with mean prediction error from 0.065 for aNDFom to 0.167 for crude fat.     

Nitrogen fertilizer application and developmental stage affect silage quality of timothy (Phleum pratense L.)

Crop composition at harvest affects the ensiling process and the resulting silage quality. The objectives of this study were to determine: (i) the effect of annual N‐fertilizer application (0, 60, 120 and 180 kg N ha^?1) and developmental stage (stem elongation, early heading, late heading and early flowering) on the ensiling properties and silage quality of the spring regrowth of timothy (Phleum pratense L.) at two sites for 1 or 2 years, and (ii) the relationship between ensiling properties of the forage and the quality of the resulting silage. Laboratory silos with wilted forage at approximately 350 g dry matter (DM) kg^?1 of fresh matter were prepared at each harvest and opened 150 d later for silage analysis. Higher rates of N‐fertilizer application decreased the concentration of water‐soluble carbohydrates (WSC), increased the buffering capacity (BC) and nitrate concentration, and decreased the ratio of WSC:BC, primarily in the early stages of development. The ensiling properties of timothy were, therefore, less favourable when high rates of N fertilizer were applied. Silage pH generally increased with increasing rates of N‐fertilizer application; this increase was particularly evident at the first three developmental stages at one site in 1 year. Non‐protein N (NPN) and soluble N concentrations of the silages increased with increased rates of N‐fertilizer application at the first three developmental stages but decreased at early flowering. Ammonia‐N concentration in the silages increased by 0·85, 0·56 and 0·67 when rates of N‐fertilizer application were 60, 120 and 180 kg ha^?1, respectively, compared with that when no N fertilizer was applied. Significant correlations between the composition of the forage ensiled and silage quality variables were found at sites in individual years but, when all data were combined, WSC concentration and BC, and their ratio in the forages, were not correlated with pH, and soluble‐N and ammonia‐N concentrations of the silages, and were weakly correlated with NPN and free amino acid‐N concentrations of the silages. Silage quality was reduced by increased N‐fertilizer application, primarily at the early developmental stages, and this can be attributed to a reduction in WSC concentration and an increase in BC of the forage. Water‐soluble carbohydrate concentration, BC, and their ratio, however, were poor predictors of silage quality.     

SILAGE AND MILK PRODUCTION. A COMPARISON BETWEEN BARLEY AND DRIED GRASS AS SUPPLEMENTS TO SILAGE OF HIGH DIGESTIBILITY

Grass silage made in late May from S23 perennial ryegrass was offered to 14 Ayrshire cows in a 16–week winter-feeding experiment. The silage had a DM content of 23.2% and contained 16–8% CP and 70.4% digestible organic matter in the DM. The silage was fed ad lib. as the sole feed in the control treatment, and supplemented with either barley or higuality dried grass cubes at the rate of 2, 3 and 4 kg/10 kg milk (2, 3 and 4 Ib/gal) in the other 6 treatments. The daily intake of silage DM was 11.6 kg/cow (25.6 lb) in the control, and significantly less in the supplement treatments. Silage intakes were higher in the dried grass than in the barley treatments. The mean daily yields of milk were 14.5 kg.cow (32.0 lb) in the control, 15.7, 16.6 and 17.0 kg (34.6.36.6 and 37.5 lb) in the barley treatments, and 17.8, 20.2 and 19.8 kg (39.2, 44.5 and 43.6 lb) in the drierass treatments, at the 2, 3 and 4 kg rates of feeding, respectively. The solidoat (SNF) content of the milk was lowest in the control treatment and highest in the drierass treatments. It is concluded that grass silage of high digestibility could be made successfully on a farm scale and that, with such a silage, a supplement of higuality dried grass was superior to a supplement of barley for milk production.     

The effect of heat-treatment and gamma radiation on the composition of unwilted and wilted lucerne silages

Three unwilted and three wilted silages were prepared from lucerne. Unwilted and wilted material was ensiled either without treatment (UWC and WC), after gamma irradiation (UWI and WI), or following heat-treatment and inoculation with Lactobacillus plantarum (UWH and WH). Gamma radiation was from a cobalt 60 source. The UWH material was heat-treated using steam, and WH material was treated with dry heat. At ensiling, irradiated material was considered to be sterile, and heat-treated material had lost 80% of its original protease activity. Fifteen 1-litre silos were prepared for each treatment (90 in total), and three from each treatment were opened after 1, 4, 14, 70 or 200 d ensiling. In irradiated silages, there was no accumulation of organic acids, but water soluble carbohydrate (WSC) concentration was almost double (116 g kg^-1dry matter) that at ensiling. This indicated extensive liberation of WSC from more complex carbohydrates during ensiling. The extent of silage fermentation in heated silages was similar to control silages. The WH silage had a higher proportion of the organic acids as lactic acid relative to the other treatments, possibly as a result of inoculation with L. plantarum. Proteolysis was unaffected by irradiation. However, heat-treatment markedly reduced the extent of proteolysis, particularly in the first four days post-ensiling. Deamination appeared to be related to the type of microbial fermentation rather than to the extent of proteolysis.     

THE DIGESTIBILITY AND PALATAB1LITY OF HIGH-MOISTURE LEGUME SILAGES WITH DIFFERENT CHEMICAL ADDITIVES

The effects of wilting and of various chemical additives on the digestibility and palatability of wet legume silages are reported. These silages showed most of the characteristics associated with the ensiling of wet herbage with a low content of soluble carbohydrate. Digestibility of all silages by wether lambs was low and exhibited little difference that might be attributed to the treatments. The untreated silage or the one sprayed with valeric/isovaleric acid, just before feeding, were more palatable to dairy heifers. Silage consumption was sharply reduced when urea was added during ensiling.