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1.
    
The amino acids (AA) and fatty acids (FA) of Beluga (Huso huso) flesh at different ages were investigated as a source of AA and FA for human consumption. The analysis of Huso huso lipids (chloroform extracted) showed significant differences (p < .05) for specific FA with age. At all ages, the major saturated FA in Huso huso fillets was palmitic acid and the major unsaturated FA were oleic and docosahexanoic acid (DHA). Protein analysis indicated that the Huso huso muscle is well balanced with good levels of essential AA and appears to be a source of high quality protein (tryptophan was not measured) with methionine/cysteine as the first limiting AA. The major AA were glutamic acid, aspartic acid, and lysine. The results obtained from the present study show that Huso huso is an excellent protein and fat source when compared with other sturgeons.  相似文献   

2.
The influence of stocking density (1, 2, 4, 6 and 8 kg m?2) was investigated on haematological parameters, growth and fin erosion of great sturgeon (Huso huso) juveniles for a period of 8 weeks. The mean weight of fish at the start of trial was 93.13±1.04 g. After 8 weeks of rearing, the mean weight was 362.4, 319.7, 267, 242.1 and 211.1 in densities 1–8 kg m?2 respectively. The results of this study showed that growth parameters, including condition factor, weight, weight gain, feed conversion ratio, specific growth rate and body weight increase, had a statistically significant difference among treatments (P<0.05). A significant difference (P<0.05) was observed in haematocrit, but the other haematological parameters, including red blood cells, white blood cells (WBC), haemoglobin concentration, mean corpuscular haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin concentration and differential WBC count, showed no significant effect with stocking density (P>0.05). At the end of the experiment, stocking density had no significant effect on plasma cortisol and glucose concentration. The fin length (dorsal, anal, ventral, pectoral and caudal) of fish was measured to calculate the fin index. According to this index, dorsal, anal, ventral and pectoral fins showed no significant difference among treatments (P>0.05), but the erosion of the caudal fin was significantly different between fish held on 6 and 8 kg m?2 (P<0.05). These results showed that rearing density has a major effect on the growth indices of H. huso. Unlike many other fish, great sturgeon exhibited lower stress responses to high stocking density. This indicates that they are more tolerable to rearing conditions in high stocking densities. With respect to the various effects that density causes on growth, fin erosion and physiological and haematological parameters, better understanding of these phenomena considering different levels of density could have a beneficial impact on many rearing steps of this species.  相似文献   

3.
    
The study on histological characteristics of the digestive system of Beluga (Huso huso) was conducted from hatching until 50 days posthatching at 16.5 °C. Development of the digestive system in this species followed the general pattern described for other Acipenserids, although there were differences in the timing of organ development among species. At hatching, the mouth was opened and digestive system was represented by a gastric cavity filled with yolk and lined by endodermal cells, and a partially differentiated hindgut. Gastric glands started to differentiate at 46.5 degree‐ days posthatching (ddph), the earliest appearance time among sturgeon fishes studied to date. At the onset of exogenous feeding (144.9 ddph), yolk sac reserves were not completely depleted in the stomach, suggesting a period of mixed nutrition. The complete development of the digestive system was not accomplished until 235.2 ddph when it showed all histomorphological features typical of juvenile specimens. According to histological results, it seems advisable to start co‐feeding H.huso larvae with inert diets at the onset of exogenous feeding, because exocrine pancreas and glandular stomach are fully differentiated, although the complete substitution of live prey by inert feed is not recommended until 235 ddph.  相似文献   

4.
    
A 17‐week feeding trial was carried out to evaluate the effects of dietary L‐carnitine level in beluga, Huso huso. A total of fish averaging 1247 ± 15.6 g (mean ± SD) were randomly distributed into 18 fibreglass tanks, and each tank holding 10 fish was then randomly assigned to one of three replicates of six diets with 50, 150, 350, 650, 950 and 1250 mg L‐carnitine kg?1 diet. At the end of 17 weeks of feeding trial, average weight gain (WG), feed efficiency (FE), protein efficiency ratio (PER) and condition factor (CF) of fish fed 350 mg kg?1 diet were significantly (P < 0.05) higher than those of fish fed 50, 150, 950 and 1250 mg kg?1 diets. WG, FE, PER and CF of beluga fed 650 mg kg?1 diet were also significantly higher than those of fish fed 50, 950 and 1250 mg kg?1 diets. Whole body and muscle protein were significantly improved by the elevation of dietary L‐carnitine level up to 350 mg kg?1. Liver superoxide dismutase and glutathione peroxidase activities of fish fed 350 and 650 mg kg?1 diets were significantly higher than those of fish fed 50, 950 and 1250 mg kg?1 diets. The dietary L‐carnitine level of 350–650 mg kg?1 diet could improve growth performance, feed utilization, protein‐sparing effects of lipid, antioxidant defence system and reproductive success. Polynomial regression of WG suggested that the optimum dietary L‐carnitine level was 480 mg kg?1 diet. Therefore, these results may indicate that the optimum dietary L‐carnitine could be higher than 350 but <650 mg kg?1 diet in beluga reared in intensive culture conditions.  相似文献   

5.
    
In this study, juvenile beluga (Huso huso) were fed diets containing different carbohydrate to lipid ratios for 5 months. At the end of the culture period, proximate compositions of the fish carcasses (moisture, protein, fat, and ash) were measured. Then, qualitative changes in the fishes were evaluated during 24-days refrigeration (4°C), along with changes in their total volatile bases nitrogen (TVN), thiobarbituric acid (TBA), free fatty acids (FFA), pH, and muscle texture profile analysis. The results of proximate analysis of the carcasses showed that moisture, protein, and ash of the carcasses increased significantly (p < 0.05) with higher carbohydrate to lipid ratio, but fat content of the carcasses decreased significantly (p < 0.05). Further, during refrigeration, TVN, TBA, and FFA increased significantly in all the samples (p < 0.05), but these changes did not follow the same pattern in all the treatments. Tissue hardness decreased during preservation. Although pH changes in the fillets were low, they were significant and without a clear-cut pattern. The results showed that use of different carbohydrate to lipid ratios in diet does not lead to clear-cut changes in qualitative characteristics of beluga fillets during refrigeration.  相似文献   

6.
为了丰富鲟鱼养殖种类,特引进了闪光鲟(Acipenser stellatus)、欧鳇(Huso huso)、西伯利亚鲟(Acipenserbaerii)和杂交鲟(西伯利亚鲟♂×俄罗斯鲟A.gueldenstaeti♀)受精卵,在直径2m的玻璃钢池中进行驯养和流水养殖,水温16-22℃,溶氧5-7mg·L-1。定期测定了上述4种鲟鱼的体长和体质量,应用计算机软件Excel和SPSS对测量的数据进行处理分析。结果表明:孵出的闪光鲟、欧鳇、西伯利亚鲟和杂交鲟鱼苗平均体质量分别为0.01349g、0.02818g、0.02016g,及0.02416g,经过60d驯化养殖,平均体质量分别达到3.80g、11.22g、7.85g,及4.19g;平均体长分别达10.61cm、13.37cm、12.98cm,及9.78cm。全长与日龄之间均呈线性关系,且R2〉0.9,全长日均增长量的大小顺序为:欧鳇〉西伯利亚鲟〉闪光鲟〉杂交鲟;体质量均与日龄呈指数函数关系,且R2〉0.9,体质量日均增长量大小顺序为:欧鳇〉西伯利亚鲟〉杂交鲟〉闪光鲟;体质量与全长的回归曲线中b〈3,均呈异速生长;肥满度分别变动在0.67~0.30、0.86~0.47、0.58~0.35,及0.71~0.44之间,均呈逐渐降低的趋势,且各个时期欧鳇肥满度最高;驯化期结束后30~40日龄生长离散最明显。  相似文献   

7.
鲟细菌性败血综合征致病菌的初步研究   总被引:4,自引:1,他引:4  
从患细菌性败血综合征的杂交鲟(Huso huso♀×Acipenser ruthenus ♂)和达氏鳇(Huso dauricus)的腹水及肾脏中共分离了5株优势菌,经人工回归感染实验证实其中2株细菌XL2-T和HK3-R具有致病性。经ATB细菌鉴定仪鉴定,结果显示:XL2-T为豚鼠气单胞菌(Aeromonas punctata subsp.caviae),HK3-R为类志贺邻单胞菌(Plesiomonas shigelloides)。这2株致病菌的主要特征为:革兰氏染色阴性,杆状,氧化酶阳性,对先锋必、妥布霉素、头孢曲松、头孢噻肟、奈替米星、新霉素等6种药物均高度敏感。  相似文献   

8.
    
Slices of Huso huso were fried, chilled, and then reheated to evaluate for the changes in lipid characteristics. The total lipid content of raw sample was 3.09 g/100 g which consists of 29.1 g/100 g saturated fatty acids (SFAs), 42.554 g/100 g monounsaturated fatty acids (MUFAs), and 28.126 g/100 g polyunsaturated fatty acids (PUFAs). In fried samples, levels of C18 fatty acid groups, MUFAs, PUFAs, and n6/n3 ratio increased while SFAs, EPA, and DHA content decreased. Upon chill storage, C18:2 fatty acid and n6/n3 ratio decreased while n3 fatty acids increased slightly. Free fatty acid (FFA) decreased after frying, but peroxide value (PV) increased with subsequent decrease at chilled condition.  相似文献   

9.
达氏鳇(♀)×西伯利亚鲟(♂)杂交幼鱼生长特性初步研究   总被引:5,自引:0,他引:5  
对野生达氏鳇(Huso dauricus)(♀)与人工养殖西伯利亚鲟(Acipenser baerii)(♂)的杂交幼鱼在人工养殖条件下的生长进行研究。结果显示:在水温14.7~16.8℃的条件下,平均全长37.43 cm、平均体重178.9 g的达氏鳇杂交种幼鱼经过80 d培育后,平均全长和平均体重分别达到51.74 cm和533.1 g。其全长与日龄呈线性关系,关系式为L=0.1683t+10.942(R2=0.9665);体重与日龄呈指数函数关系,关系式为W=24.304e0.0129t(R2=0.9421);体重与全长呈幂函数关系,关系式为W=0.0007L3.4461(R2=0.9984),其中b大于3。结果表明:达氏鳇和西伯利亚鲟的杂交种幼鱼为异速生长类型,体重生长快于全长生长。  相似文献   

10.
任华  蓝泽桥  兰大华 《河北渔业》2011,(6):44-45,53
湖北天峡鲟业有限公司利用工厂化车间培育达氏鳇、欧洲鳇亲本,经过多年精心培育,70%的达氏鳇、欧洲鳇亲鱼性腺发育成熟.2010年12月选择2尾达氏鳇雌鱼和1尾欧洲鳇雄鱼杂交成功,获得受精卵41.8万粒,孵化出优质鱼苗36.5万尾,平均受精率82%,孵化率87.5%,亲鱼成活率100%.  相似文献   

11.
人工养殖鲟鱼鱼体质量百分组成   总被引:2,自引:0,他引:2  
陈启明  胡超  陈金芳  蓝泽桥 《水产科学》2007,26(10):570-573
以俄罗斯鲟、史氏鲟、西伯利亚鲟、达氏鳇4个品种的19尾活体鱼为试验材料,按照鱼体质量>10 kg和<5 kg分组进行解剖,得出了13个解剖部位的质量分布情况。试验结果表明,鲟鱼的出肉率约50%,且史氏鲟最高,其他依次为西伯利亚鲟、俄罗斯鲟、达氏鳇。  相似文献   

12.
2011年9月底至2012年5月,采用可见植入荧光标记(VIE)和编码金属标(CWT)标志全长为(28.06±1.79)cm的达乌尔鳇(Huso dauricus),检验了VIE和CWT的标志保持率及其对达乌尔鳇生长和存活的影响。结果显示,经过210 d实验,VIE标志达乌尔鳇吻部腹面,CWT标志达乌尔鳇背部前(第1~2块背骨板间下方肌肉)、中(第6~7块后背骨板间下方肌肉)、后(第11~12块背骨板间下方肌肉)的标志保持率分别为:95.83%、92.00%、92.59%、92.59%。实验中VIE出现破碎、脱落或迁移致使标志可识别性下降;CWT背部中这一标志部位操作最为简便。标志30 d,VIE标志组的保持率较CWT背部不同部位标志组及对照组低,且差异显著(P<0.05),此后31~210 d并无显著性差异。VIE和CWT背部不同部位标志对达乌尔鳇的生长无显著性差异。结果表明,VIE和CWT均可用于标志达乌尔鳇。进行短期大规模达乌尔鳇的标志放流可使用成本相对较低的VIE标志。  相似文献   

13.
    
We examined the influence of different hatching times on growth and survival of beluga, Huso huso, over a 28-day period. Larvae from a single pair of beluga, Huso huso, were divided according to hatching time into four groups: early, mid (between 6–9 h after the first group), late (23–26 h after the first group), and a mixture of first to last hatched larvae. At hatching time, there was no statistical difference (P > 0.05) in size and length among the four groups. Prior to the onset of exogenous feeding, groups A and D exhibited slightly earlier onset of aggregation behavior and began exogenous feeding 18 h sooner than the other groups (P < 0.05). Mortality after the onset of the endogenous feeding phase was not significantly different among groups (A: 8.15% ± 0.47%; B: 8.55% ± 0.41%; C: 6.63% ± 0.53% and C: 8.23% ± 0.43%) (P > 0.05). Results showed that hatching time has no effect on the growth and overall mortality of larval beluga.  相似文献   

14.
对达氏蝗、史氏鲟人工繁殖时通过水流刺激,在水温8~13℃下,以DOS(自行研制)进行2~3次的注射催熟,再注射自制的类固醉激素17α—羟基—20β双羟基孕酮(以下简称17α—20βp);在水温16~18℃利用促黄体家释放激素类似物(LHRH—A),诱导达氏蝗、史氏鲟排卵,排精。效应时间8~18h,获得催产率100%、受精率和杂交受精率如%的效果。采用全人工饲料开口试验和苗种培育试验,并获得86.7%开口成活率和73.4%的苗种成活率。从而达到提高开口率和成活率的目的。观察鲟鲤鱼摄食、生长与形态发育状况。生长曲线表明:不论是体长还是体重,50日龄前增长缓慢,50~100日龄则速度增长较快。经100d饲养,平均体长达28.3cm,平均体重达69.7g。  相似文献   

15.
史氏鲟、达氏鳇的资源现状与研究进展   总被引:2,自引:0,他引:2  
史氏鲟(Acipenser schrencki),达氏鳇(Huso dauricus)属鲟科鱼类,是黑龙江的主要经济鱼类之一。由于近年来过度捕捞,资源遭到一定程度的破坏。本主要通过对黑龙江某江段上的捕捞生产调查统计,评估其现有资源状况,并提出了五个方面的建议。同时介绍了我国目前对鲟鱼研究方面的进展情况。1957年黑龙江水产研究所成功地进行了史氏鲟的人工繁殖试验,以后除了五十年代和七十年代的一些调查工作外,基本上中断了对这条鱼的人工繁殖试验工作。1989年,黑龙江省国营农场总局在勤得利农场建立了第一个史氏鲟放流试验站,当年就进行了人工繁殖和放流工作。四年中,向黑龙江放流鲟鱼苗累计达107万尾。在此期间有关的科研和生产单位利用这一条件开展对鲟鱼的研究,本还介绍了正在进行的“史氏鲟活体取卵手术技术研究”、“鲟幼鱼人工饲料研究”和“人工养殖技术研究”等。  相似文献   

16.
Growth performance, carcass quality, survival and haematological responses were determined when Huso huso juvenile (41.7±1.8 g) fed diets containing Ergosan (an algal product) at 0, 2.0, 4.0 and 6.0 g kg−1 for 60 days. Each diet was fed to triplicate groups of fish at 10-day intervals (1–10, 20–30 and 40–50 with non-supplemented diets and 10–20, 30–40 and 50–60 with supplemented diets). Results showed that fish fed diets containing Ergosan had significantly higher growth than the control group ( P <0.05). Survival was not different among all dietary treatments ( P >0.05). Food conversion ratio in the fish fed a diet containing 4.0 and 6.0 g kg−1 Ergosan was significantly better than the other treatments ( P <0.05), whereas protein efficiency ratio was not different between experimental diets. Lymphocyte count in the fish fed diets containing Ergosan was higher than the other treatments. Haematocrit, haemoglobin (Hb) concentration, number of erythrocytes, total leucocytes, monocyte, eosinophil, myelocyte, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were not different between treatments. Neutrophil count in the control group was higher than the fish fed supplemented diets. Furthermore, whole body lipid, moisture and fibre were not different among dietary treatments ( P >0.05) but body protein in the fish fed a diet containing Ergosan at the level of 2.0 and 4.0 g kg−1 was higher than the other treatments. Whole body ash content was higher in the control group. It was concluded that dietary administration of Ergosan can influence some growth and haematological parameters in great sturgeon, H. huso juveniles.  相似文献   

17.
    
ABSTRACT

The effects of different extraction methods (I, II, III, and IV), including two levels of acetic acid (0.2–2.0 N) and sodium hydroxide (0.2–2.0 N), on the skin gelatin extracted from Caspian Sea Huso huso skin was investigated. Furthermore, the gelatin extraction yield, melting point, viscosity, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis pattern of the solutions was studied. Based on preliminary experiments, thawed skins (weight, 50 g) were added to a flask containing four volumes of different sodium hydroxide solutions for 60 min at 7°C. Treated skins were washed thoroughly with excess cold water, rinsed with cold-distilled water, and then, treated with acetic acid. Skins were drained using cheesecloth. Afterward, the extraction of gelatin was performed in four volumes of distilled water in a water bath for 16–18 h. Results showed that the higher melting point belonged to method II, while the uppermost amount of yield and viscosity could be observed in method III. Method III had the highest level of acetic acid (2 N) and the lowest level of sodium hydroxide (0.2 N) among the other samples. High acetic acid concentration caused greater degradation of collagen cross-links, which resulted in the extraction of more protein compounds than sodium hydroxide; therefore, an increase in extraction yield and viscosity was observed. The gelatin viscosity decreased with increasing sodium hydroxide concentration (methods II and IV), while it increased with smaller amounts (methods I and III). Modelling results showed that the power-type model described the influence of soluble solids better than exponential-type model on the viscosity of the mentioned polymer.  相似文献   

18.
首先采用饱和硫酸氨分步沉淀和Sephadex G-200凝胶层析的方法,获得了非免疫状态下中华鲟(Acipenser sinensis Gray)和达氏鳇(Huso dauricus Georgi)的血清免疫球蛋白(Ig),在此基础上使用木瓜蛋白酶水解对所获得的免疫球蛋白片段进行了酶解,并采用SDS-PAGE和Western-blot等方法分析了所获得的水解片段。结果显示,2种鲟鱼的免疫球蛋白均可被木瓜蛋白酶水解蛋白,通过Sephadex G-100凝胶层析后均可得到两个完全分离的、均一的蛋白峰。SDS-PAGE检测两个水解片段的相对分子量分别为44KD和66KD,West-ern-blot检测结果显示,66KD的片段可以在硝酸纤维素杂交膜上被各自的兔抗鲟IgM多克隆抗体所识别,而44KD片段的检测结果为阴性。这表明,2种鲟科鱼类的木瓜蛋白酶水解特性相同,提示鲟科鱼类的免疫球蛋白在免疫学及生化方面具有较大的相似性。  相似文献   

19.
    
Preliminary experiments were undertaken to investigate the effects of oligofructose on beluga sturgeon (Huso huso) growth performance, survival and culturable autochthonous intestinal microbiota. Juveniles (20 g) were fed diets containing varying levels of oligofructose (10, 20 and 30 g kg?1) at 2–3% body weight per day for 7 weeks. Compared to the control group, no significant (P > 0.05) effect on growth performance was observed in fish fed diets supplemented with oligofructose at 10 and 20 g kg?1. However, compared to the 20 g kg?1 group, feeding oligofructose at 30 g kg?1 resulted in adverse effects on growth performance. Dietary supplementation of oligofructose at 20 g kg?1 significantly increased survival rate. Microbiological assessment indicated that the viable culturable autochthonous levels were not affected by dietary oligofructose. Although lactic acid bacteria (LAB) were not a dominant component of the endogenous autochthonous microbiota, LAB levels were significantly elevated in fish fed 20 g kg?1 dietary oligofructose. This elevated LAB population was able to persist for at least 1 week after reverting the prebiotic group back to a control diet. This study encourages further research on different aspects of oligofructose in sturgeon culture with clear emphasis on optimizing dosage levels.  相似文献   

20.
    
This study aimed to evaluate the antilisterial and antioxidative effects of chitosan nanocomposites and rosemary extract coating on the fillet of Huso huso inoculated with Listeria monocytogenes during refrigerated storage (4°C). Fish fillets were subjected as control (without coating), 0.5% rosemary extract (RE), 1% chitosan (CS), and combination of chitosan and rosemary extract as chitosan nanocomposite (CS/RE). Then, samples were inoculated with L. monocytogenes. Subsequently, the chemical parameters (total volatile basic nitrogen (TVB-N), peroxide value (PV), pH, and thiobarbituric acid (TBA)) and antilisterial effects of coatings were monitored during 16 days of storage at 4°C. According to the results, CS/RE demonstrated a significant (p < 0.05) ability to inhibit the growth of L. monocytogenes from 4.14 log cfu/g to 2.23 log cfu/g at the end of the storage period, followed by CT and RET treatments, respectively, compared to the control. Even though samples coated with CS/RE had the lowest pH and TVB-N values (p < 0.05), this coating was not able to pause the protein denaturation after 8 days of storage (p > 0.05) compared to the other treatments. On the other hand, CS/RE coating retarded lipid oxidation by decreasing PV and TBA production in the samples compared to the control up to the end of refrigerated storage (p < 0.05).  相似文献   

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