Removal of Vegetative Clippings Reduces Dissolved Phosphorus Loss in Runoff

Rainfall simulation was used to study the vegetative filter strip (VFS) conditions under which losses of total dissolved phosphorus (TDP) and dissolved reactive phosphorus (DRP) leaching occur. Boxes containing silt loam soil were planted with ryegrass and cut at two different intervals prior to simulated rainfall 14 days apart. Grass clippings were either removed or retained. During the second simulated rainfall, runoff TDP and DRP were greater for treatments cut the day before irrigation with clippings retained as compared to treatments cut the same day as irrigation with clippings retained. Removing clippings yielded the lowest mean TDP and DRP concentrations. Increasing the senesced vegetative surface area for contact with water, and the amount of time for leaching to occur, resulted in the greatest DRP loss. The VFS management implications should consider clipping removal or no or reduced mowing during the growing season followed by end-of-season removal to reduce DRP leaching losses.     

牧草的不同利用方式对果-草人工生态系统土壤理化及生物学性状的影响

采用单因素随机区组试验研究了果-草人工生态系统中牧草的不同利用方式对土壤理化及生物学性状的影响.结果表明;刈割覆盖、刈割压埋、畜肥还园3种利用方式均能较清耕提高土壤水分含量,降低土壤密度,稳恒土壤温度,提高土壤养分含量,增加土壤微生物数量,提高土壤酶活性.相关性分析发现,除全P与纤维分解菌、纤维分解酶、多酚氧化酶为负相关外,其余养分与生物因子间均呈正相关,且多数养分与生物因子呈显著或极显著相关;经通径分析发现,脲酶、硅酸盐细菌、纤维分解酶是促进有机质积存的主要生物因素,蔗糖酶是影响N、P、K速效养分的最主要因子,过氧化氢酶、多酚氧化酶、纤维分解菌只是选择性地对有机质的积存和N、P、K速效养分的形成起作用.     

Effects of poultry manure amendment on phosphorus uptake by ryegrass,soil phosphorus fractions and phosphatase activity

Poultry manure (PM) contains a large proportion of phosphorus (P) in mineral-associated forms that may not be readily available for plant uptake. In addition, PM application influences both chemical and biotic processes, and can affect the lability of native soil P. To investigate the effects of PM on soil P availability, we grew ryegrass (Lolium perenne) in greenhouse pots amended with poultry manure. Biomass was harvested at 4, 8, and 16 weeks following PM application, with soil separated into rhizosphere and bulk fractions. Soil was sequentially extracted by H₂O, 0.5 M NaHCO₃, 0.1 M NaOH, and 1 M HCl, and inorganic P (P_i) and enzymatically hydrolyzable organic P (P_oe) were quantitated. Root P concentrations were 37% higher and total P uptake 59% higher with PM application than Control. At week 16, there was 30% more labile-P_i (H₂O- plus NaHCO₃-P_i) in the rhizosphere with PM than in Control. Phosphodiesterase activity increased with PM application. Furthermore, acid phosphomonoesterase, alkaline phosphomonoesterase, and phosphodiesterase activities were all higher in the rhizosphere than in bulk soil at week 16 with PM, indicating that increased labile-P_i was due primarily to stimulation of soil phosphatases to mineralize NaOH-P_oe. Soil pH increased with PM application and plant growth, and may have promoted P availability by decreasing sorption of Al- and Fe-associated inorganic and organic phosphates. These results demonstrate that whereas PM application may initially increase NaOH and HCl-P_i, these fractions can be readily changed into labile-P and do not necessarily accumulate as stable or recalcitrant P in soil.     

长期有机物循环利用对红壤稻田土壤供磷能力的影响

采用盆栽试验,研究了长期不同施肥处理定位试验土壤供磷能力的差异,并从土壤磷素平衡、全磷、有机磷、Olsen-P和MB-P的含量的变化等方面探索了导致供磷能力差异的原因。结果表明,长期施用磷肥能显著提高土壤的供磷能力,其中以有机物循环利用配合磷肥施用处理土壤的供磷量能力最高,植株平均吸磷量是长期不施磷肥处理的3.5倍,比长期施用磷肥处理平均高出59.8%。长期单施氮肥导致土壤供磷能力衰竭,植株总吸磷量比长期不施肥还低17.2%,单一有机物循环利用和配施N肥植株总吸磷量比长期不施肥分别高80.3%和40.2%。有机物循环利用能明显提高土壤微生物对磷素的固持量,土壤微生物对无机磷的利用可能是其向有效磷转化的关键途径。磷肥配合系统内有机物循环利用,是提高红壤稻田土壤供磷能力的有效施肥模式。     

植物多糖类复合制剂对冬小麦光合特性及籽粒灌浆进程的影响

为探明有效成分不同的植物多糖类复合制剂的增产作用和机理,在冬小麦始花期叶面喷施以植物多糖、5-氨基乙酰丙酸和缩节胺为有效成分复配的3种复合制剂P1、P2和P3(由中国农业科学院农业环境与可持续发展研究所自主研发),以清水为对照,比较研究不同制剂对冬小麦叶绿素含量、硝酸还原酶活性、光合蒸腾特性、籽粒灌浆进程及产量等的影响。结果表明,喷施3种制剂20 d内,小麦旗叶和倒二叶的叶绿素SPAD值均较对照有不同程度增加,且随生育进程下降的趋势较对照有所延缓。旗叶净光合速率较对照增加了8.7%~17.4%,且P1和P2对SPAD值和光合速率增加的效应大于P3。此外,P1和P2还促使小麦硝酸还原酶活性增强,单叶水分利用效率增加10%以上。3种植物多糖类复合制剂均促使小麦达到最大灌浆速率的时间提前1.3~4.2 d,且P1和P2使籽粒平均灌浆速率提高6%以上。P3虽未引起籽粒灌浆速率的明显变化,但灌浆时间延长1.0 d。喷施3种复合制剂未引起小麦穗数的明显变化,但穗粒数和千粒重增加,并促使小麦增产8%以上。分析认为,3种植物多糖类复合制剂可促进小麦叶绿素合成、延缓叶片衰老、改善旗叶光合潜能和籽粒灌浆进程,进而实现增产。     

Effect of indigenous and introduced vesicular-arbuscular mycorrhizal fungi on growth and phosphorus uptake of Trifolium pratense and on inorganic phosphorus fractions in a cambisol

Summary Five selected vesicular-arbuscular mycorrhizal (VAM) fungi and the native population of a cambisol were tested in sterilized soil conditions, with Trifolium pratense as host plant. Indigenous fungi were the most effective in enhancing plant growth and P uptake, which were correlated with a higher root colonization. Selected fungi did not spread further in the root after 4 months from sowing, occupying less than 10% at the end of the experiment; inoculation with Glomus fasciculatum E3 yielded a higher dry-matter production than any other VAM species, but did not significantly increase shoot P concentration above that of the non-mycorrhizal control. Interactions between indigenous and introduced VAM fungi were studied in unsterilized soil. Results from fresh and dry weights of shoots and the percentage of fungal infection showed that the native endophytes competed more efficiently in colonizing the root. Inoculation with selected VAM species did not improve plant growth. Sterilization altered the inorganic P fractions of the soil, particularly those extracted with NH₄F and NaOH. Sterilized soil contained less inorganic P than unsterilized soil, but more soluble P. By the end of the experiment in sterilized soil, P extracted with NH₄Cl, NH₄F and NaOH and total inorganic P were significantly different among inoculation treatments, suggesting that VAM fungi may differ in their ability to take up P.     

Contribution of Pseudomonas mendocina and Glomus intraradices to aggregate stabilization and promotion of biological fertility in rhizosphere soil of lettuce plants under field conditions

A field‐plot experiment was undertaken to compare the effect of inorganic fertilizer with that of inoculation with an arbuscular mycorrhizal fungus, Glomus intraradices, or with a plant‐growth‐promoting rhizobacterium, Pseudomonas mendocina, alone or in combination with inorganic fertilizer, on plant growth and nutrient uptake by lettuce. The influence of the microbial inoculation treatments on soil physical, biochemical and biological properties was also assessed. Two months after planting, fertilizer and inoculation with G. intraradices or P. mendocina had significantly increased shoot and root biomass and foliar nutrient contents (P, Fe). The inoculation with G. intraradices or P. mendocina both increased the soil water‐soluble carbohydrates and the percentage of stable aggregates. In this study, we provide the first evidence of the beneficial effect of a plant‐growth‐promoting rhizobacterium on soil aggregate stabilization under field conditions. Only inoculation with P. mendocina had a significant effect on the dehydrogenase and phosphatase activities, 21 and 89%, respectively, compared with the control. Inorganic fertilization alone did not increase aggregate stability or enzyme activities in soil, even though this treatment produced the largest increases in mass of lettuce.     

Clopyralid and Compost: Formulation and Mowing Effects on Herbicide Content of Grass Clippings

The presence of clopyralid, a selective broadleaf herbicide, in compost near Spokane, Washington in 2000 was attributed to grass clippings collected from area lawns and subsequently used as compost feedstock. A field study was conducted in 2001 to evaluate the effects of herbicide formulation and mowing practice on the clopyralid content of grass clippings. The objective was to develop guidelines that would limit the concentration of clopyralid in clippings, thereby reducing the amount of clopyralid entering the compost production stream. Clopyralid was applied to turfgrass as either a sprayable (S) or granular (G) formulation. Grass clippings were either collected in a bagging lawn mower and removed from the plots or returned into the plant canopy using a mulching mower designed to finely chop and disperse the leaf blades. Clippings were sampled for a period of ten weeks after application, and again in the summer of 2002, and analyzed for clopyralid content. Mowing treatment had no significant effect on clopyralid content of grass clippings. The S formulation resulted in higher concentration than the G at 4 hours after treatment (193 mg kg^?1 and 53 mg kg^?1, respectively). At 10 weeks after treatment (WAT), clopyralid concentration averaged 0.9 mg kg^?1, and cumulative recovery of clopyralid in grass clippings was 35% and 29% of the amount applied for the S and G formulations, respectively. By 56 and 98 WAT, clopyralid concentration in clippings was 0.06 and 0.02 mg kg^?1, respectively. Based on these results, and depending on feedstock dilution and composting conditions, a waiting period of up to one year after application of clopyralid could be necessary for treated grass clippings to be safely used as compost feedstock.     

Does accelerated soil organic matter decomposition in the presence of plants increase plant N availability?

Plant roots can increase microbial activity and soil organic matter (SOM) decomposition via rhizosphere priming effects. It is virtually unknown how differences in the priming effect among plant species and soil type affect N mineralization and plant uptake. In a greenhouse experiment, we tested whether priming effects caused by Fremont cottonwood (Populus fremontii) and Ponderosa pine (Pinus ponderosa) grown in three different soil types increased plant available N. We measured primed C as the difference in soil-derived CO₂-C fluxes between planted and non-planted treatments. We calculated “excess plant available N” as the difference in plant available N (estimated from changes in soil inorganic N and plant N pools at the start and end of the experiment) between planted and non-planted treatments. Gross N mineralization at day 105 was significantly greater in the presence of plants across all treatments, while microbial N measured at the same time was not affected by plant presence. Gross N mineralization was significantly positively correlated to the rate of priming. Species effects on plant available N were not consistent among soil types. Plant available N in one soil type increased in the P. fremontii treatment but not in the P. ponderosa treatment, whereas in the other two soils, the effects of the two plant species were reversed. There was no relationship between the cumulative amount of primed C and excess plant available N during the first 107 days of the experiment when inorganic N was still abundant in all planted soils. However, during the second half of the experiment (days 108-398) when soil inorganic N in the planted treatments was depleted by plant N uptake, the cumulative amount of primed C was significantly positively correlated to excess plant available N. Primed C explained 78% of the variability in plant available N for five of the six plant-soil combinations. Excess plant available N could not be predicted from cumulative amount of primed C in one species-soil type combination. Possibly, greater microbial N immobilization due to large inputs of rhizodeposits with low N concentration may have reduced plant available N or we may have underestimated plant available N in this treatment because of N loss through root exudation and death. We conclude that soil N availability cannot be determined by soil properties alone, but that is strongly influenced by root-soil interactions.     

Effect of straw management, tillage timing and timing of fertilizer nitrogen application on the crop utilization of fertilizer and soil nitrogen in an irrigated cereal rotation

In irrigated grain-growing soils on Canada's prairies, straw management can affect nitrogen (N) fertility and long-term soil organic matter reserves. We conducted a 2-year field experiment in southern Alberta, on a Dark Brown Chernozemic Lethbridge loam (Typic Boroll), to determine the effects of straw removal, tillage, and fertilizer timing on crop uptake of soil and fertilizer N. During the study (1991 and 1992), the crop was oat (Avena sativa L.) and wheat (Triticum aestivum L.), respectively, in an experiment that had been in a wheat-wheat-oat-wheat rotation since 1986. Five straw-tillage treatments were: straw-fall plow, straw-pring plow, no straw-fall plow, no straw-spring plow and no straw-direct seeding. Fertilizer N was applied in fall or spring. Ammonium nitrate (5 at.% ¹⁵N) was added at 100 kg N ha⁻¹ in fall 1990 or spring 1991. For oat (1991), plant N derived from soil was higher under fall plow than under spring plow, higher with tillage than direct seeding, and unaffected by straw removal. The plant N derived from fertilizer was not affected by straw removal in fall plow treatments, but under spring plow, it was higher with straw removal. The plant N derived from fertilizer showed a significant straw-tillage × fertilizer timing interaction; with fall incorporated straw, plant N derived from fertilizer was 44.0 kg N ha⁻¹ for spring-applied, and 30.6 kg N ha⁻¹ for fall-applied N, but in other straw-tillage treatments there was no effect of fertilizer timing. Cumulative fertilizer N recovery (plant + soil) over the 2 years averaged 64.2%, and was unaffected by straw-tillage treatment. Fertilizer N recovery, however, was less with fall-applied N (61.3%) than spring applied N (66.8%). At mid-season, fall plow treatments had higher soil inorganic N and inorganic N derived from fertilizer than spring plow treatments, apparently because of less immobilization. The fall plow treatments also retained higher inorganic N after harvest. Straw removal and fertilizer timing did not influence soil inorganic N and soil inorganic N derived from fertilizer. N removal in straw (16 kg N ha⁻¹ yr⁻¹) could deplete soil N in the long-term. Long-term effects of tillage timing on soil N will depend on the relative amount of N lost by leaching with fall plowing and that lost by denitrification under spring plowing. With direct seeding, crop yield and uptake of soil N was less, and N losses by denitrification could be greater. Application of N in spring, rather than fall, should enhance crop N uptake, reducing N losses and enhancing long-term soil organic N.     

Comparison of DTPA and resin extractable soil Zn to plant Zn uptake

Abstract

Extraction of soil zinc with routine chemical extractants does not always reflect differences in Zn availability as detected by plant uptake. This study was undertaken to explore and compare the use of an ion exchange resin and diethylenetriaminepentaacetic acid (DTPA) for extracting soil Zn as related to plant Zn uptake. Beans were grown in 1989 following differential cropping with corn and beans or fallow in 1988 on a Portneuf silt loam near Kimberly, Idaho. Two Zn fertilizer treatments were imposed across previous cropping treatments. A batch method for determining resin extractable soil Zn was established.

Both plant Zn concentration and Zn uptake by beans in 1989 were significantly higher in Zn fertilized than unfertilized treatments regardless of previous crop; and higher in plots previously cropped with corn than beans or fallow, regardless of Zn treatment. DTPA and resin extractable soil Zn were significantly higher in Zn fertilized plots compared to unfertilized plots but did not differ between previous cropping treatments. Resin and DTPA extractable soil Zn concentrations were positively correlated. Resin extracted soil Zn correlated better with plant Zn concentration and Zn uptake throughout the growing season than DTPA extracted soil Zn, particularly in plots that had been fallowed or previously cropped with corn. Resin may be extracting labile soil Zn not extracted with DTPA and, therefore, be better simulating plant uptake. Both extraction methods correlated better with Zn uptake when evaluated within cropping treatments, emphasizing the need to consider previous crop when calibrating soil tests.     

施肥对板栗林土壤 CH4 吸收通量动态的影响

为了探明无机肥和有机肥施用对板栗林地土壤 CH4 吸收通量动态的影响,探讨板栗林地土壤 CH4 通量与环境因子之间的关系,在浙江省临安市典型板栗林样地布置施肥试验。于2011年6月~2012年6月期间,采用静态箱-气相色谱法测定了不施肥（CK）、 无机肥（IF）、 有机肥（OF）和有机无机混合肥（OIF）处理下土壤CH4 吸收通量的全年动态变化,并测定了土壤温度、 土壤水分、 水溶性有机碳（WSOC）和微生物量碳（MBC）含量。结果表明,板栗林土壤CH4 吸收通量呈现明显的季节性变化,最大值出现在9月,最小值出现在2~3月;施肥处理均显著抑制了土壤 CH4 的吸收,具体表现为 IF、 OF和OIF处理下土壤CH4年吸收量与CK处理［CH4 3.09 kg/(hm2a)］相比分别减少了7.0%、 1.6% 和 4.4%。此外,施肥显著增加了土壤WSOC和MBC含量（P 0.05）,且施肥使土壤碱解氮、 铵态氮、 硝态氮、 全氮和有机质含量均有不同程度的增加。相关性分析表明,土壤CH4 吸收通量与土壤表层5 cm处温度之间呈显著正相关（P 0.05）,但与土壤水分、 MBC含量之间没有显著相关性;土壤CH4 吸收与土壤WSOC含量之间（除CK处理外）均具有显著相关性（P 0.05）。因此,施肥引起土壤理化性质［如 NH+4-N、 NO-3-N、 全氮（TN）、 有机碳（SOC）等］和 WSOC 含量的改变可能是施肥显著抑制了板栗林土壤CH4排放的主要原因。     

调控措施对滨海盐渍土磷素形态及作物磷素吸收的影响

滨海盐渍化土壤存在磷素有效性低的问题。本试验采用根袋法盆栽试验,共设不施磷肥、常规磷肥、磷肥+生物质炭、磷肥+腐殖酸、磷肥+商品有机肥5个处理,分析不同调控措施对非盐渍土、轻度盐渍土和中度盐渍土有效磷含量、磷素形态以及大麦磷素吸收利用的影响。结果表明:①盐碱障碍降低根区内外土壤有效磷含量,表现为非盐渍土轻度盐渍土中度盐渍土。添加生物质炭能显著提高轻度、中度盐渍土根区内外土壤有效磷含量,较常规磷肥对照处理分别提高40.72%、84.80%。②盐碱障碍降低大麦产量,抑制地上部对磷素的吸收,不同调控措施均能促进盐渍土上大麦对磷素的吸收,提高磷肥利用率。轻度盐渍土上不同调控措施的增产效果不显著,中度盐渍土上添加生物质炭处理显著提高大麦产量,较常规磷肥对照处理提高63.20%。③盐碱障碍降低土壤活性无机磷、NaOH-Pi、NaHCO_3-Po、NaOH-Po比例,增加HCl-Pi比例。添加生物质炭处理能显著提高盐渍土活性无机磷比例,提高土壤磷的有效性。添加生物质炭和商品有机肥处理对中度盐渍土上HCl-Pi比例的降低效果优于轻度盐渍土。     

Long‐term effects of manure and fertilization on soil organic matter and quality parameters of a calcareous soil in NW China

Long‐term applications of inorganic fertilizers and farmyard manure influence organic matter as well as other soil‐quality parameters, but the magnitude of change depends on soil‐climatic conditions. Effects of 22 annual applications (1982–2003) of N, P, and K inorganic fertilizers and farmyard manure (M) on total organic carbon (TOC) and nitrogen (TON), light‐fraction organic C (LFOC) and N (LFON), microbial‐biomass C (MB‐C) and N (MB‐N), total and extractable P, total and exchangeable K, and pH in 0–20 cm soil, nitrate‐N (NO ‐N) in 0–210 cm soil, and N, P, and K balance sheets were determined using a field experiment established in 1982 on a calcareous desert soil (Orthic Anthrosol) at Zhangye, Gansu, China. A rotation of irrigated wheat (Triticum aestivum L.)‐wheat‐corn (Zea mays L.) was used to compare the control, N, NP, NPK, M, MN, MNP, and MNPK treatments. Annual additions of inorganic fertilizers for 22 y increased mass of LFON, MB‐N, total P, extractable P, and exchangeable K in topsoil. This effect was generally enhanced with manure application. Application of manure also increased mass of TOC and MB‐C in soil, and tended to increase LFOC, TON, and MB‐N. There was no noticeable effect of fertilizer and manure application on soil pH. There was a close relationship between some soil‐quality parameters and the amount of C or N in straw that was returned to the soil. The N fertilizer alone resulted in accumulation of large amounts of NO ‐N at the 0–210 cm soil depth, accounting for 6% of the total applied N, but had the lowest recovery of applied N in the crop (34%). Manure alone resulted in higher NO ‐N in the soil profile compared with the control, and the MN treatment had the highest amount of NO ‐N in the soil profile. Application of N in combination with P and/or K fertilizers in both manured and unmanured treatments usually reduced NO ‐N accumulation in the soil profile compared with N alone and increased the N recovery in the crop as much as 66%. The N that was unaccounted for, as a percentage of applied N, was highest in the N‐alone treatment (60%) and lowest in the NPK treatment (30%). In the manure + chemical fertilizer treatments, the unaccounted N ranged from 35% to 43%. Long‐term P fertilization resulted in accumulation of extractable P in the surface soil. Compared to the control, the amount of P in soil‐plant system was surplus in plots that received P as fertilizer and/or manure, and the unaccounted P as percentage of applied P ranged from 64% to 80%. In the no‐manure plots, the unaccounted P decreased from 72% in NP to 64% in NPK treatment from increased P uptake due to balanced fertilization. Compared to the control, the amount of K in soil‐plant system was deficit in NPK treatment, i.e., the recovery of K in soil + plant was more than the amount of applied K. In manure treatments, the recovery of applied K in crop increased from 26% in M to 61% in MNPK treatment, but the unaccounted K decreased from 72% in M to 37% in MNPK treatment. The findings indicated that integrated application of N, P, and K fertilizers and manure is an important strategy to maintain or increase soil organic C and N, improve soil fertility, maintain nutrients balance, and minimize damage to the environment, while also improving crop yield.     

部分根区干燥灌溉条件下土壤温度和玉米N吸收改善研究

Soil temperature is a major effective factor on the soil and plant biological properties. Irrigation can affect soil temperature and thereby induces a temperature effect on plant growth, which may result in an economic increase due to higher yield and plant nutrition. A field experiment was carried out to investigate the effects of three irrigation strategies including full irrigation (FI), partial root-zone drying (PRD) and deficit irrigation (DI) on soil temperature and the consequent results on the grain yield and N uptake of maize (Zea May L.). Soil temperature was measured by time domain reflectometry (TDR) sensors during the 2010 growing season. Irrigation treatments were applied from 55 to 107 d after planting. The PRD treatment caused soil temperature to be in a favorable domain for a longer period (for over 60% of the measuring dates) as a consequent result of water movement to deeper soil layers compared with the other treatments; the PRD treatment also reduced soil temperature at deeper soil depths to below the maximum favorable soil temperature for maize root growth, which resulted in deeper root penetration due to both water availability and favorable soil temperature. Compared to the FI treatment, the PRD treatment increased root water uptake by 50% and caused no significant reduction in total N uptake, while this was not observed in the DI treatment partially due to the negative temperature effect of DI on plant growth, which consequently affected the water and nutrient uptake. A longer vegetation period in the PRD treatment was observed due to higher leaf N concentrations and no significant reduction in maize grain yield occurred in the PRD treatment, compared with those in the FI treatment. Based on the results, having 15.2% water saving during the whole growing season, the PRD irrigation would positively affect soil temperature and the water and nutrient uptake as a consequent, which thereby would prevent significant reduction in maize grain yield.     

Phosphorus availability and rice grain yield in a paddy soil in response to long-term fertilization

Rice (Oryza sativa L.) is one of the most important crops in the world, and its production is limited by soil phosphorus (P) deficiencies in many parts of the world. Impacts of long-term fertilization regimes on rice productivity and soil P availability is largely unknown. A long-term (26-year) field experiment in a paddy soil of southeastern China was carried out to study the response of rice grain yield and soil P pools to different fertilization regimes including control without fertilization (CK), nitrogen, P, and potassium (NPK) fertilizer (NPK), NPK fertilizer plus cattle manure (NPKM), and NPK fertilizer plus rice straw (NPKS). Application of fertilizers (NPK, NPKM, and NPKS) increased rice grain yield compared with the CK treatment (on average, by 75%, 97%, and 92%, respectively). Soil P was predominately present in the organic form (51–75% of total P) across different treatments. Most soil inorganic P fractions decreased with time due to continuous depletion by rice plants in the nonfertilized treatment (CK), while they generally increased with time in the fertilizer treatments (NPK, NPKM, and NPKS) due to continued supply of P to soil. On the other hand, soil organic P fractions increased continuously with time regardless of treatment, probably due to the retention of stubble and biological immobilization of inorganic P. Positive relationships between the rice grain yield and most inorganic P fractions in the CK treatment indicated the P limitation for rice production due to no P inputs and long-term conversion of inorganic P into organic P.     

Soil phosphorus fractions after 111 years of animal manure and fertilizer applications

Accumulated soil P in agricultural soils is a major source of soluble and particulate forms of P entering water resources and degrading water quality. However, few research sites are currently available to evaluate the long-term effects of different cropping systems and fertility practices on soil inorganic and organic P accumulation. The objectives of this study were: (1) to compare the forms and quantity of different inorganic and organic soil P fractions in plots on Sanborn Field, which has been cultivated for 111 years; and (2) to assess the use of standard soil test P extractants for determining changes in soil P dynamics over time. A modified sequential P extraction procedure was used to separate labile and stable inorganic and organic P pools from surface soils collected on Sanborn Field in 1915, 1938, 1962, and 1999 from plots in continuous corn, continuous wheat, continuous timothy, and a corn-wheat-clover rotation amended with either manufactured fertilizers, horse or dairy manure or receiving no fertilization since 1888. Additional samples were collected from a native grass prairie site of a similar soil series to estimate soil characteristics at Sanborn Field before initial cultivation in 1888. Observed accumulation of Bray-1 P among fertilizer and manure treatments was attributed to over-application of P due to unrealistically high yield goals for each cropping system. Long-term cultivation of Sanborn Field increased soil bulk density and lowered soil pH and total organic C compared with native prairie. Fertilization either by addition of manufactured fertilizer or manure significantly increased inorganic resin-P and inorganic NaOH-extractable P. Applications of animal manure also significantly increased most organic P fractions compared with the unfertilized treatment. The native prairie had a larger proportion of total P in organic forms compared with cultivated plots, especially in organic NaOH-extractable P, but no significant decreases in either residual or total P were observed due to cultivation. This study confirms that soil P availability in cropping systems that are amended with predominantly organic P amendments may differ from conventional cropping systems relying on manufactured P fertilizers. However, no direct evidence was found to support the hypothesis that any individual inorganic or organic soil P fraction has a better relationship than conventional soil test P extractants with plant P uptake under contrasting organic and conventional fertility practices.     

An examination of potential extraction methods to assess plant-available organic phosphorus in soil

The role of soil organic phosphorus (P) in plant nutrition was assessed using data from a glasshouse pot experiment carried out on seven soil types using two contrasting plant species (Lolium perenne, Pinus radiata) and 12 different extractants (five salts (0.025 M ethylenediaminetetraacetic acid (EDTA), 0.025 M EDTA pH 7, Olsen, Mehlich-III, and 6% NaOCl pH 7.5) and seven exchange resins (Hampton chelating resin, Bio-Rad Chelex-100, Dow MAC-3, Amberlite IRC76, Diaion WT01S, Lewatit MP500A, Diaion WA30)). The contribution from mineralization of soil organic P was inferred by consistent increases in correlation coefficients between extractable P and plant P uptake when organic P was considered in addition to inorganic P. The best correlated extractants for combined inorganic and organic P were NaOCl (r = 0.84), Hampton chelating resin (r = 0.78), and MP500A resin (r = 0.73), which compared favorably with Olsen P (r = 0.66) and EDTA (r = 0.72). ³¹P nuclear magnetic resonance analysis of selected extracts from two soils confirmed that the Hampton-chelating-resin-extractable P was mainly monoester and diester forms of organic P, while there was no monoester or diester organic P in the IRC76 resin extract—poorly correlated with plant uptake. The findings of this study suggest that readily extractable forms of organic P in soil contribute to short-term plant P uptake, and this P should be considered for inclusion in routine tests for soil P availability.     

分室磷添加下菌根对滇池流域红壤间作玉米生长及磷素利用的影响

随着全球范围内磷矿资源短缺问题的日益严重,间作或菌根技术强化作物对土壤磷(P)的利用及增产增收的效应受到越来越多的关注。通过三室隔网盆栽模拟试验研究了分室磷处理[不添加磷(P0)、添加有机磷(OP50)、添加无机磷(IOP50)]和根室不接种(NM)、根室接种丛枝菌根真菌Glomus mosseae(GM)对与大豆间作的玉米的生长及磷素利用的影响。研究结果表明:所有复合处理中,以间作?GM?IOP50组合处理下的玉米根系最短和地上部生物量最高;OP50处理下,间作玉米的菌根侵染率显著高于单作处理。间作条件下,无论分室磷添加与否,接种GM处理的玉米地上部生物量明显高于NM处理;接种GM处理的玉米根系生物量和株高均显著高于NM处理,且根系生物量以间作?GM?OP50组合处理下最高。接种GM条件下,P0、IOP50、OP50处理下的间作植株生物量较单作处理分别提高45.98%、111.33%、33.56%。单作条件下,无论分室磷添加与否,接种GM处理的玉米地上部磷含量均显著高于NM处理;无论何种种植模式及分室磷添加与否,接种GM处理的植物根系磷含量均显著高于NM处理。无论磷添加与否,间作?GM组合条件下的玉米地上部磷吸收量均显著较高,其中IOP50处理下的地上部磷吸收量显著高于OP50处理。间作?GM组合条件下,IOP50处理玉米根系的磷吸收效率均显著高于OP50处理。可见,接种GM、分室磷添加和间作各自在一定程度上促进了玉米的生长。综合菌根侵染、生物量及磷含量与吸收量、磷吸收效率等指标,所有复合处理中以间作?GM?IOP50组合对玉米地上部的促生作用最好,玉米磷素吸收最多,可望有效强化滇池流域红壤坡耕地磷素的利用。     

栽培模式及施肥对玉米和大豆根际土壤磷素有效性的影响

栽培模式及施肥管理对作物吸收利用土壤磷素的影响较大,本研究为探明玉米/大豆套作系统作物根系交互作用下根际土壤无机磷组分动态变化特征,利用盆栽试验测定了玉米/大豆套作(M/S)、玉米单作(MM)和大豆单作(SS)3种栽培模式以及不施肥(CK)、施氮钾肥(NK)和施氮磷钾肥(NPK)3种施肥处理下玉米和大豆地上部生物量及吸磷量和根际与非根际土壤速效磷、无机磷组分含量,以期为优化玉米/大豆套作系统磷素管理提供理论依据。研究结果表明同一施肥水平下,套作玉米的籽粒产量显著高于单作玉米;施磷显著提高了单作玉米籽粒产量,而对套作玉米籽粒产量影响不大。无论施肥与否,套作大豆秸秆及籽粒产量均高于单作大豆。所有施肥处理均表现为套作模式下单株作物地上部磷积累量显著高于单作模式。玉米成熟期,CK、NK处理下套作玉米根际土壤速效磷含量分别比单作玉米高54.2%和71.8%;大豆始花期,NPK处理下套作大豆根际土壤速效磷含量比单作大豆高19.8%。大豆成熟期,NK、NPK处理下套作大豆根际土壤速效磷含量分别比单作大豆高23.8%和108.0%。无论是单作还是套作模式,玉米根际土壤Al-P含量在3个施肥处理下均低于非根际土壤。CK和NK处理下单作玉米根际土壤Al-P含量分别是套作玉米的1.19倍和1.22倍;NPK处理下单作玉米根际土壤Fe-P含量是套作玉米的1.21倍。在CK、NK和NPK施肥处理下,单作大豆根际土Al-P含量分别是套作大豆1.12倍、1.30倍和1.25倍,单作大豆非根际土Al-P含量分别是套作大豆的1.22倍、1.30倍和1.06倍。CK、NK处理下单作大豆根际土壤Fe-P含量分别是套作大豆的1.47倍和1.12倍。研究得出结论,低磷条件下,与单作相比,玉米/大豆套作更有利于作物对土壤Al-P、Fe-P的活化吸收。