Nitrogen fixation in association with the root systems of goldenrods (Solidago L.)

The roots of seven species of goldenrod (Asteraceae: Solidago) were assayed for associative nitrogen fixation. We used the acetylene reduction method to measure N₂ fixation rates in sampling jars that contained roots of sampled plants and soil. The rate of C₂H₂ reduction in jars with S. rigida (12.9 ± 1.2 nmol h⁻¹, mean ± SE) and S. canadensis (6.0 ± 2.0 nmol h⁻¹) was significantly higher than in soil-only control jars. There was measurable C₂H₂ reduction in jars of the other five species, but the rates were not significantly different from controls. The rates of C₂H₂ reduction per root dry weight and per total plant dry weight also were higher for S. rigida and S. canadensis than for the other species.     

Wet and dry deposition of phosphorus into Lake Huron

The deposition rates for wet, dry, and integrated fallout of P to the southern portion of Lake Huron were determined. Samples were analyzed for water-soluble, acid (pH =2) soluble, and insoluble (bound) P. The term ‘available P’ was used to indicate the sum of the water- and acid-soluble fractions of P in each type of input. Of the integrated fallout samples, approximately one-third was present as available P. The deposition rate for dry fallout of available P was determined to be 1.7 ng cm^?2 day^?1. The wet deposition rate was estimated to be 2.2 ng cm^?2 day^?1 for available P. Total fallout of available P, determined from shore-based integrated fallout collectors, was calculated to occur at a rate of 6.5 ng cm^?2 day^?1. The difference between the wet-plus-dry deposition and the integrated deposition rates has been ascribed to the contamination of integrated fallout collectors by local sources (roads, agricultural activity, etc.) though attempts were made to minimize this problem. The presence of large quantities of biological material (pollen, algae, insect larvae) accounted for a large fraction of the difference observed, especially in the spring months.     

Radiological maps for fallout 7Be in forest soil horizons in a mountainous area of Turkey

Due to the paucity of data on separate spatial distribution of cosmogenic ⁷Be radionuclide activities in forest soil layers, a spatial study was performed in Mount Ida (Kazdagi)/Edremit, Turkey. In this study, it was aimed to examine the ⁷Be spatial variability and depth distribution in the surface soil layers. The ⁷Be activity concentrations were determined by HPGe gamma spectrometry system and the distributions of ⁷Be activities in the O_L and O_F + O_H horizons throughout the region were mapped separately. Activity concentrations of ⁷Be in O_L horizons and O_F+O_H horizons varied as 35 ± 23–701 ± 40 Bq kg^?1 and 0.96 ± 0.63–197 ± 11 Bq kg^?1, respectively. ⁷Be inventories (0.20 ± 0.06–5.69 ± 0.75 kBq m^?2) in the study area were relatively higher when compared with the other regions of the world. ⁷Be inventories increased with altitude, slope and the thickness of the humus layer in some of the investigated area. Average ⁷Be activity level in deciduous forest stand type was significantly higher than that for coniferous and mixed stand types. Our limited data could not provide a latitudinal distribution pattern of ⁷Be soil inventory or precipitation dependence and further investigation is needed.     

Oxygen consumption of the earthworm species Dendrobaena mrazeki

Dendrobaena mrazeki is an endemic earthworm species inhabiting dry habitats such as pine and thermophilous oak forests in Central Europe. Metabolically, D. mrazeki showed some features typical for endogeic species and some of epigeic ones. In comparison with the related Dendrobaena octaedra, D. mrazeki was a larger earthworm with fresh body mass of adult and subadult individuals of W = 0.59 ± 0.05 g. Its body mass-specific oxygen consumption (M/W = 48 ± 5 μl O₂ g^?1 h^?1, at 15 °C) was the lowest of all earthworms studied (Aporrectodea caliginosa, Aporrectodea rosea, D. octaedra, Lumbricus castaneus, Lumbricus rubellus and Octolasion lacteum), being strongly dependent on W (b from the equation M/W = aW^b about ?0.8). D. mrazeki had low relative water content (77.4% of fresh body mass) and small relative amount of dry weight of the intestinal content (20.1% of dry body mass), which is similar to the epigeic D. octaedra. The respiration rate of D. mrazeki remained the lowest even after recalculating M/W to respiration rate per dry mass or per dry mass without the intestine content to correct for the differences among species in body water content and gut content.     

Concentrations of Mercury in Wild Growing Higher Fungi and underlying Substrate near Lake Wdzydze,Poland

Fourteen species of wild growing mushrooms and surface (0–10 cm) soils were collected near Lake Wdzydze in the northern part of Poland in 1996–1997 to understand the status of mercury pollution. Concentrations of mercury in mushrooms varied between 100±30 and 2400±1900 ng g^-1 dry matter in caps and 60±1 and 1300±1500 ng g^-1 dry matter in stalks. Concentrations of mercury in underlying soil were between 30±1 and 140±120 ng g^-1 dry matter (between 36±18 and 63±100 ng g^-1 depending on the soil type). Bioconcentration factors (BCF: concentrations in mushroom/concentrationin soil) of total mercury were between 2.3±1.1 and 90±110 for caps, and between 2.1±1.0 and 53±56 for stalks. Scaly tooth (Sarcodon imbricatum) contained the greatest concentrations of mercury in the flesh. However, there was no significant relationship (p >: 0.05) between mercury content in the fruiting bodies of this speciesto soil mercury concentrations. A significant (p < 0.01) positive relationship between mercury content in caps to underlying soil was noted for European cow bolete (Suillus bovinus), while a negative relationship between mercury content in caps and stalks to underlying soil was observed for Sandy knight-cap (Tricholomaflavovirens), Shaggy scale-head (Pholiota squaroso-adiposa),Gypsy mushroom (Rozites caperata) and Pine spike cap (Chroogomphus rutilus).     

Calculation of radionuclide ground deposition by means of measurements on sewage sludge

The concentrations of various radionuclides have been measured in the incoming water, the outgoing water and the sludge from the sewage treatment plant serving the town of Lund in southern Sweden. The mean residence time for the water in the plant is 1 to 2 days, whereas for the sludge it is 3 to 4 weeks. Variations in the residence time, which may influence the efficiency of the plant, are related to the season of the year and the load on the plant. Measurements show that for ⁷Be, ⁵¹Cr, ¹³⁴Cs, and ¹³⁷Cs between 37 and 56% of the incoming activity leaves the plant with the sludge. For ¹⁰⁶Ru and ¹³¹I these figures lie between 6 and 14%. The deposition of ⁷Be has been measured at Lund and the relation between the deposition on the ground (Bq m^?2) and the activity concentration in the sludge (Bq kg^?1 dry weight) has been studied and found to by relatively constant at ?(0.8 ±0.2) kg m^?2. In measurements on sewage sludge, the detection limit for deposition of ⁷Be on the ground is around 16 Bq m^?2.     

Comparative studies of radionuclides from global fallout and local sources in ground level air and sewage sludge

The presence of activation products in the environment released to the air from a nuclear power station has been studied in sewage sludge and ground level air. The measured time variation of the ⁶⁰,Co concentration in sludge and ground level air was found to be in good agreement with the reported variation in release rate to the air from the power station when the prevalent wind direction is taken into account. The ratio between the ⁷,Be normalized concentration in sludge and ground level air was studied and was found to be about 1 for radionuclides due to global fallout but less than 2 · 10^?3, for ⁶⁰,Co. Our conclusion is that ⁶⁰,Co spreads from a local source (a nuclear power station) and to a considerable degree is dry deposited on the ground and then washed off by rain.     

Accumulation of acridine from water,food, and sediment by the fathead minnow,Pimephales promelas

Azaarenes are one of several classes of organic compounds which contain mutagenic and carcinogenic substances that are found in synthetic fuels effluents. This study investigated the potential for a mutagenic azaarene, acridine, to accumulate in freshwater fish (Pimephales promelas) via four possible pathways: (1) direct uptake from water, (2) uptake via interaction with contaminated sediments, (3) uptake via ingestion of contaminated zooplankton (Daphnia pulex), and (4) uptake via ingestion of benthic invertebrates (Chironomus tentans) living in contaminated sediments. The results showed that acridine was rapidly accumulated from water by fathead minnows. Equilibrium concentration was attained within 24 h at a concentration factor ([acridine]_{fish, wet wt}/[acridine]_water) of 125±10. Depuration was rapid and appeared to occur in two stages, with a net elimination rate of 0.23 h^?1 [acridine]_fish at equilibrium. Equilibrium concentration factors of 51±5, 30±2, and 874±275 were observed forChironomus, Daphnia, and sediment, respectively. The calculated rates of uptake of acridine via ingestion of contaminated invertebrates (0.02 μg g^?1 h^?1) and ingestion of sediment (0.01 μg g^?1 h^?1) were negligible compared with direct uptake from water (1.40 μg g^?1 h^?1) in a hypothetical system with all compartments in equilibrium.     

Sampling soil and sediment depth profiles at a fine resolution with a new device for determining physical,chemical and biological properties: the Fine Increment Soil Collector (FISC)

Purpose

Many environmental investigations (empirical and modelling) and theories are based on reliable information on the depth distribution of physical, chemical and biological properties in soils and sediments. However, such depth profiles are not easy to determine using current approaches, and, consequently, new devices are needed that are able to sample soils and sediments at fine resolutions.

Materials and methods

We have designed an economic, portable, hand-operated surface soil/sediment sampler—the Fine Increment Soil Collector (FISC)—which allows for the close control of incremental soil/sediment sampling and for easy recovery of the material collected by a simple screw-thread extraction system. This innovative sampling system was developed originally for the beryllium-7 (⁷Be) approach in soil and sediment redistribution research. To ensure reliable estimates of soil erosion and sediment deposition from ⁷Be measurements, the depth distribution of this short-lived fallout radionuclide in soil/sediment at the resolution of millimetres is a crucial requirement. This major challenge of the ⁷Be approach can be met by using the FISC.

Results and discussion

We demonstrate the usefulness of the FISC by characterising the depth distribution of ⁷Be at increments of 2.5 mm for a soil reference site in Austria. The activity concentration of ⁷Be at the uppermost increment (0–2.5 mm) was ca. 14 Bq kg^?1 and displayed decreasing activity with depth. Using most conventional sampling devices (i.e. the scraper-plate system), the most accurate depth increment would have been 10 mm, and the activity concentration at the surface would have been considerably lower. Consequently, coarser sampling would have influenced estimates of ⁷Be-derived soil erosion and deposition. The potential application for other soil/sediment properties, such as nutrients (e.g. phosphorus), contaminants and carbon are also discussed.

Conclusions

By enabling soil and sediment profiles to be sampled at a depth resolution of millimetres, the FISC has the potential to provide key information when addressing several environmental and geoscientific issues, such as the precise depth distributions of soil/sediment nutrients, contaminants and biological properties.     

Migration path and isotope tracing of 137Cs and 239 + 240Pu in estuary sediments: a case study of Liao River estuary in China

Purpose

Currently, the distribution characteristics and transport processes of the radionuclides ¹³⁷Cs and ^239?+?240Pu in the sediments of estuaries are still a controversial issue. Thus, in the present study, we investigated the distribution characteristics, sources and migration path of ¹³⁷Cs and ^239?+?240Pu in sediment cores of the Liao River estuary (LRE), China, and evaluated the relative contributions of the Pacific Proving Grounds (PPG) and riverine sources of Pu for the sediments in this area. We used ¹³⁷Cs and Pu isotopes for dating the sediments and estimated the sedimentation rates by the two radionuclides in the LRE.

Materials and methods

Seven sediment samples were collected using a box corer from the Liao River estuarine wetland and tidal flat in the LRE in October 2012 and April 2015, respectively. The activities of ¹³⁷Cs in the various samples were determined by γ spectrometry using HP-Ge detectors with 60% relative counting efficiency. Approximately 2–5 g of sediment were spiked with ²⁴²Pu (ca. 1 mBq) as a chemical yield tracer for Pu isotopic analysis at the School of Radiation Medicine and Protection, Soochow University. The measurement of Pu isotopes (²³⁹Pu, ²⁴⁰Pu, ²⁴²Pu) was performed by a sector field ICP-MS. In order to quantitatively differentiate the relative proportions of global fallout and PPG, we employed a two-end member mixing model to estimate their contributions.

Results and discussion

The average values of ¹³⁷Cs, ^239?+?240Pu activity concentrations, and ²⁴⁰Pu/²³⁹Pu atom ratios in the surface sediment samples of the LRE were 6.727?±?0.251 mBq g^?1, 0.294?±?0.024 mBq g^?1, and 0.188?±?0.049 (1σ), respectively. The average ²⁴⁰Pu/²³⁹Pu atom ratios ranged from 0.180?±?0.034 to 0.199?±?0.021 in sediment cores from the east and west sides of the LRE. For core LT-2 values for ¹³⁷Cs and ^239?+?240Pu concentrations were from below the detection limit: 3.380?±?0.414 and 0.036?±?0.007 to 0.105?±?0.007 mBq g^?1. The mean ²⁴⁰Pu/²³⁹Pu atom ratio 0.217?±?0.050 (1σ) in sediment core LT-2 lies between the global fallout and PPG close-in fallout.

Conclusions

We found that atmospheric fallout is the main source of Pu in sediment cores from the east and west sides of the LRE. For core LT-2, atmospheric fallout and riverine input (~?73.4%) are the major sources of Pu with the source of the rest of Pu (~?26.6%) attributed to the PPG via the Tsushima Warm Current and the coastal water of the East China Sea. The sedimentation rates (means 0.62–0.8 cm year^?1) estimated by the two radionuclides were in good agreement and without any statistically significant difference.

     

Quantitative monitoring of airborne lead pollution by a foliose lichen

The potential use of lichens as quantitative monitors of airborne heavy metal pollution has been demonstrated by measurements of Po²¹⁰, Pb, Cd, Zn, and V levels in sequential growth of the foliose lichen Pseudoparmelia baltimorensis from three localities in the greater Washington, D.C. area. Samples were dated using the growth rate measured by Lawrey and Hale (1977). Po²¹⁰, Pb, and V increase with age in a given colony. Higher levels of Pb, V, Cd, and Zn are present in samples from the two more polluted sites. The retention of heavy metals is consistent with trapping by an ion exchange mechanism. Since Po²¹⁰ is the daughter product of in situ decay of Pb²¹⁰, accumulated from atmospheric fallout of known rate, its levels can be used to calculate trapping efficiencies of Pb and retrospective fallout rates. These rates are similar to published data from rural and urban areas.     

Effects of Biosolids Based Soil Products on Soil Physical and Chemical Properties in Urban Gardens

A study was conducted to evaluate the effect of two biosolids based soil products on soil physical properties in urban community gardens in Tacoma, Washington. The Tagro soil product is made from Class A biosolids cake mixed with sand and sawdust. The GroCo biosolids compost is produced from biosolids cake and sawdust. Both products meet regulatory requirements for unrestricted use and are locally available to gardeners. Plots were established in 3 community gardens and maintained for 2 growing seasons. Amendments were applied at 200 Mg ha^?1 dry weight in the first year of the study. Plots were split for the 2^nd year with half of the amendment plots receiving an additional 200 Mg ha^?1 of amendments. Although lower in most metals than the soils they were added to, biosolids did not have a significant effect on total soil metal concentrations. Biosolids addition increased the water infiltration rate from 10.1 ± 0.95 ml min^?1 in control soils to between 51 ± 6.1 and 212 ± 34 ml min^?1, depending on the product used. Bulk density was decreased from 1.07 ± 0.01 g cm^?3 to between 0.51 ± 0.08 and 0.77 ± 0.03 g cm^?3. Total carbon, nitrogen and available phosphorus were increased in the biosolids amended plots in comparison to the control plots. Biosolids addition resulted in decreased pH (5.35-5.75) in comparison to the control soils (6.43). The results from this study indicate that biosolids-based soil amendments can be a suitable amendment for urban community gardens.     

Gut load and transit time in Hormogaster elisae (Oligochaeta,Hormogastridae) in laboratory cultures

The gut load and gut transit time (GTT) of the endogeic earthworm Hormogaster elisae in laboratory cultures at 18 °C and 23 ºC were studied. The GTT, 5.25 h ± 0.40 at 18 ºC and 3.63 h ± 0.46 at 23 ºC, was determined by staining the soil with alimentary colouring (tartrazine). The gut load was calculated with two methods: earthworm mass difference, before and after voiding the gut, and dry mass of the gut content. The gut load ranged between 168 and 261 mg wet mass g live earthworm mass^–1 (mass difference method) or 137–196 mg dry mass g live earthworm mass^–1 (dry mass method). With the obtained data a potential annual soil turnover for H. elisae was calculated: 211–470 kg wet soil mass kg live earthworm mass^–1 year^–1 (mass difference method) or 176–325 kg dry soil mass kg live earthworm mass^–1 year^–1 (dry mass method).     

Mineralization of nitrogen compounds in the soil under the bilberry-sphagnum birch forest (Yaroslavl Oblast)

Net mineralization of nitrogen was measured in the horizons of peaty podzolic gley soil (At1, 0–6 cm, At2, 6–10 cm, and A2, 10–20 cm) of the bilberry-sphagnum birch forest from May to the beginning of November in 2011 and 2012; it comprised 2.1 ± 0.4, 2.7 ± 0.4, 1.2 ± 0.4, and 1.9 ± 0.2, 1.9 ± 0.2, 0.8 ± 0.15 g N/m², respectively. The total of 6 ± 0.7 and 4.6 ± 0.3 g N/m² were mineralized in the soil profile in the years 2011 and 2012, respectively, with the contribution of ammonification being more than 99%. The seasonal variations in nitrogen mineralization correlated with the temperature and soil moisture content in At1 and At2 horizons. The efficiency of nitrogen mineralization in soil horizons under the birch forest was expressed by similar values, namely, 0.005 ± 0.0008, 0.0064 ± 0.0008, and 0.003 ± 0.001 mg N/g C per day, and this suggests a high efficiency of substrate utilization in the eluvial part of the soil profile. The seasonal variation of carbon dioxide production correlated with the ammonification in the At1 horizon with r = 0.85 and p = 0.03 in 2011 and 2012, and in the At2 horizon with r = 0.65 and p = 0.16 in 2012. Over the studied period, production of ammonia depended on soil moistening in the dry year of 2011 (r = 0.91; p = 0.01) and soil temperature in the wet year of 2012 (r = 0.78; p = 0.06). The productivity of the process equaled 3 ± 0.9 mg N/m², or 30 g N/ha in both years of observation.     

Transfer of Cd, Pb, Ra and U from Phosphogypsum Amended Soils to Tomato Plants

About 170 million tons of phosphogypsum (PG) are annually generated worldwide as a by-product of phosphoric acid factories. Agricultural uses of PG could become the main sink for this waste, which usually contains significant radionuclide (from the ²³⁸U-series) and toxic metals concentrations. To study PG effects on pollutant uptake by crops, a completely randomised greenhouse experiment was carried out growing Lycopersicum esculentum Mill L. on a reclaimed marsh soil amended with three PG rates (treatments), corresponding to zero (control without PG application), one, three and ten times the typical PG rates used in SW Spain (20 Mg ha^?1). The concentrations of Cd, Pb, U (by inductively coupled plasma mass spectroscopy) and ²²⁶Ra and ²¹⁰Po (by γ-spectrometry and α-counting, respectively) were determined in soil, vegetal tissue and draining water. Cadmium concentrations in fruit increased with PG rates, reaching 44?±?7 μg kg^?1 formula weight with ten PG rates (being 50 μg kg^?1 the maximum allowed concentration by EC 1881/2006 regulation). Cd transfer factors in non-edible parts were as high as 4.8?±?0.5 (dry weight (d.w.)), two orders of magnitude higher than values found for lead, lead, uranium and radium concentrations in fruit remained below the corresponding detection limits—0.5 and 0.25 mg kg^?1 and 0.6 mBq kg^?1, respectively (in a d.w. basis). ²³⁸U (up to 7 μg kg^?1 d.w.) and ²¹⁰Po (up to 0.74 Bq kg^?1 d.w.) could be measured in some fruit samples by α-spectrometry. Overall, the concentrations of these metals and radionuclides in the draining water accounted for less than 1% of the amount applied with PG.     

Persistence,survival, vertical dispersion,and horizontal spread of the biocontrol agent,Penicillium oxalicum strain 212, in different soil types

The potential adverse effects of a biological control agent can be assessed from the knowledge on its environmental fate and behaviour. This study focuses on environmental fate and behaviour of Penicillium oxalicum Currie and Thom strain 212 (PO212), a promising biocontrol agent, after its application to three different soil substrates under different crop conditions in terms of the persistence, survival, vertical dispersion, and horizontal spread. Two different PO212 conidial formulations, one without any additives (F1) and one with additives (F2), were applied to the seedbeds of tomato plants seven days before their transplanting into three soil types: sterilized peat in an experimental glasshouse and the sandy loam and the loamy sand soils in two commercial orchards. The size of the Penicillium spp. population in the substrate samples was quantified using the classical microbiological method of counting of number of colony-forming units on a semi-selective culture medium. The size of PO212 biomass in the samples was quantified by a specific quantitative real-time polymerase chain reaction. We found that PO212 had a very limited vertical dispersion and horizontal spread in the three soil substrates that were tested in the study. PO212 persisted in the natural soil substrates for at least one year at very low levels (0.45–61.2 ng dried PO212 biomass/g dry soil or 2.71–367.2 ng fresh PO212 biomass/g dry soil). Treatment influenced the horizontal spread of the PO212 biomass in the sterilized peat and sandy loam soils. We did not find significant increases in the size of indigenous Penicillium spp. population (10²–10³ CFU/g dry soil) in the three soil substrates following application of the F1 or F2 conidial formulations. In the sandy loam soil of the orchard in the VO2009 field trial the Penicillium spp. population decreased at increasing depths. Although PO212 persists in the soil after its application, it does not proliferate. We conclude that PO212 is an effective and safe biocontrol agent to control soil-borne pathogens.     

Application of the caesium‐137 technique to soil degradation studies in the Southwestern Highlands of Ethiopia

Soil degradation is a serious problem in the central and northern Highlands of Ethiopia. It has been so for several decades as a result of over exploitation and mismanagement. Relocation of a portion of the population from these regions to the relatively less populated Southwestern Highlands has taken place for decades to try to address the problem. However, such mass resettlements have caused severe soil degradation problems in many destination areas in the Southwestern Highlands. The aim of this study was to assess the problem of soil degradation using the caesium‐137 isotope and to test its value for erosion study in the region. The adapted USLE was applied to compare results from the caesium‐137 isotope studies. Along a deforestation continuum, fields cultivated for various years were studied for erosion. From a reference grazing land plot, total caesium‐137 fallout of 2026 ± 176 Bq m⁻² with a CV of 24·6 per cent was recorded showing the presence of sufficient fallout to apply the technique. Erosion in cultivated fields was estimated against this reference using conversion models. Results from the Proportional Model |−13·9 ± 2·7|and the adapted USLE |12·3 ± 2·6| were not significantly different (p < 0·05), meaning the technique provides reliable results. A positive relationship was observed between severity of erosion and time of cultivation after forest clearing (R² = 0·78). The mean annual loss of soil from cultivated land, 14·9 ± 2·9 t ha⁻¹ y⁻¹, is already beyond the tolerable threshold and might exacerbate further clearing of forests for cultivation if the land is not properly managed. Copyright © 2011 John Wiley & Sons, Ltd.     

The effect of ph on fungitoxic interactions between a solvent and pesticide

The effect of pH on interactions between combinations of the solvent acetone and the pesticide captan was determined using the fungiPythium ultimum, Sclerotinia homeocarpa, andPestalotia sp.. Seven concentrations of the solvent acetone, ranging from 0.1 to 3.0 % (v/v), were interacted with four concentrations of the fungicide captan, ranging from 1.0 to 10.0 ppm (mg L^?1). This interaction procedure was repeated at pH 4.5, 5.5, 6.5, and 7.5, using a temperature of 30 °C. Acetone and captan interacted synergistically towardsP. ultimum andS. homeocarpa, and antagonistically towardsPestalotia sp., regardless of the pH. However, the solvent concentration at which synergism or antagonism was first observed usually decreased as pH increased. The actual pH response obtained was dependent upon both the captan level and culture used. As pH increased from 4.5 to 7.5, the toxicity of captan decreased by up to 40% withS. homeocarpa andPestalotia sp., and 80% withP. ultimum. WithS. homeocarpa andPestalotia sp., the magnitude of synergism or antagonism increased as the captan concentration was raised from 1.0 or 2.5 ppm up to 7.5 or 10.0 ppm. With P. ultimum, the degree of synergism decreased at pH 4.5 and 5.5, but increased at pH 6.5 and 7.5, as the captan concentration was raised from 2.5 to 10.0 ppm. The lowest interaction magnitudes were recorded at pH 4.5 forP. ultimum, but was variable for the other cultures. The greatest interaction magnitudes were obtained at pH 4.5 forS. homeocarpa, 5.5 forPestalotia sp., and 6.5 or 7.5 forP. ultimum.     

Simultaneous measurement of soil organic and inorganic carbon: evaluation of a thermal gradient analysis

Purpose

The best method for determining soil organic carbon (SOC) in carbonate-containing samples is still open to debate. The objective of this work was to evaluate a thermal gradient method (ThG), which can determine simultaneously inorganic carbon (SIC) and SOC in a wide range of soil samples.

Materials and methods

The determination of SOC by ThG (SOC_ThG) was compared to the following widespread standard methods: (1) acidification (ACI) as pretreatment and subsequent dry combustion (SOC_ACI) and (2) volumetric quantification of SIC by a calcimeter (CALC) and subtraction of the total carbon content as determined by dry combustion (SOC_CALC). Precision (F test) and bias (t test) were tested on a subset of seven samples (n?=?3). Comparison of the ThG and CALC methods was performed by regression analysis (n?=?76) on samples representing a wide range of SOC (5.5 to 212.0 g kg^?1) and SIC (0 to 59.2 g kg^?1) contents.

Results and discussion

Tests on the replicated subset showed that the precision of ThG was not significantly different from ACI or CALC (F values?<?39, n?=?3) for SOC and SIC measurements. However, SOC_ACI and SOC_CALC contents were systematically and significantly lower compared to SOC_ThG contents. The positive bias for SOC_ThG relative to SOC_CALC contents appeared also in the regression analysis (given numbers?±?standard errors) of the whole data set (y?=?(4.67?±?0.70)?+?(0.99?±?0.01)x, R ²?=?0.99, n?=?76). When performing a regression with carbonate-free samples, the bias between the methods was negative (?2.90?±?0.63, n?=?29) but was positive in the set with carbonate-containing samples (3.95?±?1.41, n?=?47). This observation corroborated the suspicion that the use of acid for carbonate decomposition can lead to an underestimation of SOC.

Conclusions

All methods were suitable for differentiation between SIC and SOC, but the use of acid resulted in lower estimates of SOC contents. When comparing soil samples with different carbonate concentrations, the use of the ThG method is more reliable.     

Beryllium phytotoxicity in soybeans

A greenhouse study was conducted to assess the effects of soil-applied beryllium (Be) on the growth and Be content of soybeans [Glycine max (L.) Merr.], grown on acid southeastern soils under limed and unlimed conditions. This study was conducted using a factorial design, with two soil types varying in clay content (Blanton sand, a loamy, siliceous, thermic Grossarenic Paleudult; and Orangeburg loamy sand, a loamy, siliceous, thermic Typic Paleudult), two soil treatments (limed and unlimed) and five Be concentrations (0, 25, 50, 100, and 150 mg Be kg^?1 t soil). Addition of Be to unlimed Blanton soil had the most toxic effects of all treatment combinations; at the 150 mg Be kg^?1 treatment plant biomass was reduced as much as 90% and plant Be concentration was as high as 226 mg Be kg^?1. Beryllium concentrations were greater in plants grown in a soil low in clay (Blanton soil). Liming of soils treated with Be resulted in lowered tissue Be concentrations in plants grown on either soil type.