Effects of biogas slurry application on peanut yield,soil nutrients,carbon storage,and microbial activity in an Ultisol soil in southern China

Purpose

Biogas slurry (BS) was known to influence soil–plant ecosystems when applied as a fertilizer, especially in combination with a chemical fertilizer (CF). Limited information was available regarding how this combination of BS–CF actually affected the soil–plant ecosystems. The purpose of this study was to evaluate the effects of BS–CF combinations on peanut yield, soil properties, and carbon (C) storage in a red soil (Ultisol) in southern China.

Materials and methods

The soil was fertilized with five treatments, including a control (T1), CF-only (T2) treatment, and three treatments with different BS–CF combinations (T3–T5). The final quantities of N/P₂O₅/K₂O applied in T2–T5 were 120:90:135 kg ha^?1. In T3–T5, 15 % (18 kg ha^?1), 30 % (36 kg ha^?1), and 45 % (54 kg ha^?1) of total N (TN), respectively, were applied with BS and the remaining TN was applied with CF. Crop yield, soil nutrients, C storage, and microbial activity were determined through field and laboratory experiments.

Results and discussion

In the field experiment, peanut grain yields of T3–T5 were higher than those of T1 (44.5–55.7 %) and T2 (10.8–19.4 %), with the highest yield from T4 (3588 kg ha^?1). The relationship between BS–TN inputs and peanut grain yield conformed to the linear-quadratic equation: y?=??1.14x ²?+?59.1x?+?2988 (R ²?=?0.98). The biomasses of peanut plants, at the flowering, pod production, and harvesting stages, were higher in T4 compared with those in T1 and T2. Moreover, T4 produced higher soil N and P (total and available) concentrations at the pod production and harvesting stages relative to other treatments, with increased soil microbial biomass C and N, and enhanced dehydrogenase and urease activities, at the flowering, pod production, and harvesting stages. Data from the incubation experiment were fitted to a first-order kinetic model, which showed that although the application of BS increased potentially mineralizable C, the additional C seemed to slowly degrade, and so would be retained in the soil for a longer period.

Conclusions

A BS–CF combination increased peanut grain yield and biomass, due to increases in soil N and P availability, microbial biomass C and N concentrations, and urease and dehydrogenase activities. Moreover, the organic C retention time in the red soil was extended. Combined application of BS–CF at a suitable ratio (36 kg BS–TN ha^?1), together with proper management practices, could be effective to improve the quality and nutrient balance of amended soils.

     

Changes of labile and recalcitrant carbon pools under nitrogen addition in a city lawn soil

Purpose

Carbon (C) flux is largely controlled by the highly bio-reactive labile C (LC) pool, while long-term C storage is determined by the recalcitrant C (RC) pool. Soil nitrogen (N) availability may considerably affect changes of these pools. The aim of this study was to investigate the effects of N treatments on soil LC and RC pools.

Materials and methods

A field experiment was conducted in a city lawn soil for 600 days with three N treatments, i.e., the control (0 kg N ha^?1 year^?1), low-N (100 kg N ha^?1 year^?1), and high-N (200 kg N ha^?1 year^?1) treatments. As the N source, NH₄NO₃ solution was added to soil surface monthly. Measurements of LC, RC, and other soil biochemical properties, including pH, soil respiration rates, microbial biomass, and enzymes activities, were taken during the experiment period.

Results and discussion

The low-N and high-N treatments increased 6.3 and 13% of the LC pool, respectively, which was caused by decreased microbial biomass and soil respiration rates under the N treatments. By contrary, the low-N and high-N treatments decreased 5.9 and 12% of the RC pool, respectively. The N addition treatments enhanced phenol oxidase activities. The enhanced oxidase activities decreased new RC input and the increased dissolved organic C stimulated RC pool decomposition. The LC and RC pools were highly influenced by the N treatments, whereas effect of the N treatments on soil organic C was not significant. The N addition treatments also caused soil acidification and reduced bacterial biomass proportion in the soil microbial composition.

Conclusions

The N addition increased the LC pool but decreased the RC pool in the soil. These changes should greatly impact soil long-term C storage.     

Bulk density and content,density and stock of carbon,nitrogen and heavy metals in vegetable patches and lawns of allotments gardens in the northwestern Ruhr area,Germany

Purpose

In view that soils are bodies and that processes such as storage and release of water, carbon, nutrients and pollutants, and aeration and rooting happen in these bodies, it is of interest to know the density of elements and compounds in soils. On the basis of soil bulk and element density of organic carbon (OC), N, and heavy metals in soils and of horizon thickness, stocks of these elements for garden soils were calculated.

Materials and methods

Fourteen gardens in four allotments of the northwestern part of the Ruhr area, Germany were investigated. The research included 14 vegetable patches, 13 lawns, 2 compost heaps, and 1 meadow. Volume samples were taken. The soil analysis included pH, soil bulk density, and OC, N, Pb, Cd, Zn, Cu, and Ni contents.

Results and discussion

The soils were from sandy loam to loamy sand. The pH was slightly acid and C/N ratio about 20. Soil bulk density was between 0.8 and 1.4 g cm^?3 and mean bulk density was 1.1 g cm^?3. Mean OC content was for compost 7.4 %, vegetable patches 5.2 % (0–30 cm depth), and lawns and meadow 5.8 and 5.2 % (0–5 cm depth). OC density for compost was 76 mg cm^?3, vegetable patches 56 mg cm^?3, and lawns 67 mg cm^?3 (0–5 cm). Mean OC stock in 0–30 cm soil depth in vegetable patches was 16.4 kg m^?2, lawns 15.5 kg m^?2, and meadow 11.1 kg m^?2. N contents were between 0.06 and 0.46 %. For compost, the mean was 0.39 %, vegetable patches 0.27 % (0–30 cm), lawn 0.28 %, and meadow 0.26 % (0–5 cm). Mean stock of N in 0–30 cm depth for vegetable patches was 0.84 kg m^?2, lawn 0.76 kg m^?2, and meadow 0.55 kg m^?2. For heavy metals in compost, vegetable patches, lawn and meadow, Cd contents were in the range of 1.7 to 3.0 mg kg^?1, Pb 49 to 152 mg kg^?1, and Zn 52 to 1830 mg kg^?1. The amounts stored per square meters in 30 cm depth were for Cd 0.6–1.1 g, Pb 15–52 g, Zn 41–440 g, Cu 4–39 g, and Ni 1–8 g.

Conclusions

Allotment gardens have a high capacity to store CO₂ as OC. Roughly, there will be 7–8 million tons of OC stored in the 1.3 million allotment gardens of Germany. The high amount of 8000 kg N ha^?1 could damage the groundwater when released by wrong soil management. Cd, Zn, Pb, Cu, and Ni amounts of 7.8, 1000, 300, 135, and 30 kg ha^?1, respectively, are a lasting burden.

     

The impact of different forest types on phytolith-occluded carbon accumulation in subtropical forest soils

Purpose

Occlusion of carbon in phytoliths is an important biogeochemical carbon sequestration mechanism and plays a significant role in the global biogeochemical carbon cycle and atmospheric carbon dioxide (CO₂) concentration regulation at a millennial scale. However, few studies have focused on the storage of phytolith and phytolith-occluded carbon (PhytOC) in subtropical forest soils.

Materials and methods

Soil profiles with 100-cm depth were sampled from subtropical bamboo forest, fir forest, and chestnut forest in China to investigate the variation of phytoliths and PhytOC storage in the soil profiles based on amass-balance assessment.

Results and discussion

The storage of phytoliths in the top 100 cm of the bamboo forest soil (198.13?±?25.08 t ha^?1) was much higher than that in the fir forest (146.76?±?4.53 t ha^?1) and chestnut forest (170.87?±?9.59 t ha^?1). Similarly, the storage of PhytOC in the bamboo forest soil (3.91?±?0.64 t ha^?1) was much higher than that in the fir forest soil (1.18?±?0.22 t ha^?1) and chestnut forest soil (2.67?±?0.23 t ha^?1). The PhytOC percentage in the soil organic carbon pool increased with soil depth and was the highest (4.29 %) in the bamboo forest soil. Our study demonstrated that PhytOC in soil was significantly influenced by forest type and the bamboo forest ecosystem contributed more significantly to phytolith carbon sequestration than other forest ecosystems.

Conclusions

Different forest types have a significant influence on the soil PhytOC storage. Optimization of bamboo afforestation/reforestation in future forest management plans may significantly enhance the biogeochemical carbon sink in the following centuries.

     

Effects of Residual Mulch Film on the Growth and Fruit Quality of Tomato (<Emphasis Type="Italic">Lycopersicon esculentum</Emphasis> Mill.)

The quantities of residual mulch film in the soil will further increase with the wide application of agricultural plastic mulch film, and the pollution of residual mulch film, which is a continuous pollutant and the one that is difficult to degrade, is a major limiting factor for the sustainable development of agriculture in China. Residual mulch film in the soil inevitably affects soil hydrodynamic parameters, destroys the homogeneity of the soil texture, seriously impedes the movement of soil water and solutes, and thus greatly influences crop growth and fruit quality. To unravel the effects of residual mulch film on tomato growth and fruit quality, pot experiments in the greenhouse were carried out in 2015 and 2016 in Northwest China. Six levels of residual mulch film were applied: 0 kg ha^?1 (CK), 80 kg ha^?1 (T1), 160 kg ha^?1 (T2), 320 kg ha^?1 (T3), 640 kg ha^?1 (T4), and 1280 kg ha^?1 (T5). Plant height, stem diameter, dry biomass, yield, root length, root surface area, fruit shape index (FSI), soluble sugar content (SSC), organic acid (OA), vitamin C (VC), lycopene, and nitrate content (NC) were measured. Plant height, stem diameter, dry biomass, and yield of tomato had a downward trend as the residual mulch film amount increased. Root length and root surface area were significantly decreased with an increasing amount of residual mulch film, but root volume and root diameter showed an inconspicuous decrease. When the amount of residual mulch film was more than 80 kg ha^?1, growth indexes, dry biomass, and yield of tomato showed a sharp decline. FSI, OA, and lycopene decreased as the residual mulch film amount increased, whereas SSC, VC, and NC showed an increase trend. With the increase in residual mulch film amount, the F and membership function values (X _μ ) all showed a declining trend in comparison to the CK. Therefore, residual mulch film can aggravate the negative effects on the comprehensive fruit quality of tomato.     

The accumulation of phytolith-occluded carbon in soils of different grasslands

Purpose

A better understanding of the role of grassland systems in producing and storing phytolith-occluded carbon (PhytOC) will provide crucial information in addressing global climate change caused by a rapid increase in the atmospheric CO₂ concentration.

Materials and methods

Soil samples of typical steppe, meadow steppe, and meadow in Inner Mongolia, China, were taken at 0–10-, 10–20-, 20–40-, and 40–60-cm depths in July and August of 2015. The soil phytoliths were isolated by heavy liquid (ZnBr₂), and the soil PhytOC was determined by the traditional potassium dichromate method.

Results and discussion

The results of our study showed that the storage of soil phytoliths was significantly higher in the meadow (33.44 ± 0.91 t ha^?1) cf. meadow steppe (26.8 ± 0.98 t ha^?1) and typical steppe (21.19 ± 4.91 t ha^?1), which were not different. The soil PhytOC storage was significantly different among grassland types, being: meadow (0.39 ± 0.01 t ha^?1) > meadow steppe (0.29 ± 0.02 t ha^?1) > typical steppe (0.23 ± 0.02 t ha^?1). PhytOC storage in typical steppe soil within the 0–60-cm soil layer is the lowest and that in meadow soils is the highest. The grassland type and the soil condition play significant roles in accumulation of phytoliths and PhytOC in different grassland soils. We suggest that the aboveground net primary productivity (ANPP) is important in soil phytolith accumulation and PhytOC content.

Conclusions

Phytolith and PhytOC storages in grassland soil are influenced by factors such as grass type, local climate and soil conditions, and management practices. Management practices to increase grass biomass production can significantly enhance phytolith C sequestration.

     

<Emphasis Type="Italic">Trichoderma</Emphasis> improves the growth of <Emphasis Type="Italic">Leymus chinensis</Emphasis>

Bio-fertilizer application has been proposed as a strategy for enhancing soil fertility, regulating soil microflora composition, and improving crop yields, and it has been widely applied in the agricultural yields. However, the application of bio-fertilizer in grassland has been poorly studied. We conducted in situ and pot experiments to investigate the practical effects of different fertilization regimes on Leymus chinensis growth, with a focus on the potential microecological mechanisms underlying the responses of soil microbial composition. L. chinensis biomass was significantly (P?<?0.05) increased by treatment with 6000 kg ha^?1 of Trichoderma bio-fertilizer compared with other treatments. We found a positive (R² =?0.6274, P <?0.001) correlation between bacterial alpha diversity and L. chinensis biomass. Hierarchical cluster analysis and nonmetric multidimensional scaling (NMDS) revealed that soil bacterial and fungal community compositions were all separated according to the fertilization regime used. The relative abundance of the most beneficial genera in bio-fertilizer (BOF) (6000 kg ha^?1Trichoderma bio-fertilizer) was significantly higher than in organic fertilizer (OF) (6000 kg ha^?1 organic fertilizer) or in CK (non-amend fertilizer), there the potential pathogenic genera were reduced. There were significant negative (P?<?0.05) correlations between L. chinensis biomass and the relative abundance of several potential pathogenic genera. However, the relative abundance of most beneficial genera were significantly (P?<?0.05) positively correlated with L. chinensis biomass. Soil properties had different effects on these beneficial and on these pathogenic genera, further influencing L. chinensis biomass.     

Changes in the pH,EC, available P,SOC and TN stocks in a single rice paddy after long-term application of inorganic fertilizers and organic matters in a cold temperate region of Japan

Purpose

The objective of this study was to determine the changes in the main soil chemical properties including pH, electrical conductivity (EC), available phosphorus (P), soil organic carbon (SOC) and total nitrogen (TN) stocks after long-term (31 years) additions of two types of organic matters—rice straw and rice straw compost, combined with NPK fertilizers in single rice paddy in a cold temperate region of Japan.

Materials and methods

A long-term experiment on combined inorganic fertilizers and organic matters in paddy rice cultivation began in May 1982 in Yamagata, northeastern Japan. After the 31st harvest, soil samples were collected from five treatments [(1) PK, (2) NPK, (3) NPK + 6 Mg ha^?1 rice straw (RS), (4) NPK + 10 Mg ha^?1 rice straw compost (CM1), and (5) NPK + 30 Mg ha^?1 rice straw compost (CM3)] at five soil depths (0–5, 5–10, 10–15, 15–20, and 20–25 cm). Soil chemical properties of pH, EC, available P, SOC, and TN were analyzed.

Results and discussion

The pH decreased significantly only at the higher compost rate of 30 Mg ha^?1, while EC increased in all the organic matter treatments. Available P significantly increased in the CM1 and CM3 treatments by 55.1 and 86.4 %. The amounts of SOC stock increased by 67.2, 21.4, and 8.6 %, and soil TN stock by 64.1, 20.2, and 8.5 % in CM3, RS, and CM1, respectively, compared to NPK treatment.

Conclusions

Significant changes in soil properties were observed after 31 years of organic matter applications with reference to PK- and NPK-fertilized rice paddy soils. A significant decrease in pH was observed with the application of a high rate (30 Mg ha^?1) of rice straw compost but not with the conventional rate of 10 Mg ha^?1. However, EC increased significantly relative to that of the PK- and NPK-fertilized plots in all the organic matter treatments. Available P significantly increased in the CM1 and CM3 treatments by 55.1 and 86.4 %. The amounts of SOC stock expressed as a percentage of total C applied to the soil were higher from 10 Mg ha^?1 compost (28.7 %) than that from 6 Mg ha^?1 rice straw (17.4 %), indicating a more effective soil organic C accumulation from rice straw compost than that from original rice straw.

     

Modeling of Soil-Plant-Fertilizer Relationships for Optimizing Fertilizer Doses for Different Levels of Finger Millet Yield under Semi-Arid Alfisols

Experiments were conducted to test the superiority of treatment combinations of nitrogen (N; 0, 50, 100, 150, 200 kg ha^?1), phosphorus (0, 30, 60, 90 kg ha^?1) and potassium (0, 30, 60 kg ha^?1) for finger millet during 2005–2007. Application of 200-90-60 kg ha^?1 gave maximum yield of 1666, 1426 and 1640 kg ha^?1 in 3 years, respectively. The yield regression model through soil and fertilizer nutrients gave predictability of 0.98, 0.97 and 0.98, with sustainability yield index (SYI) of 50.4, 49.4 and 52.5 in 2005, 2006 and 2007, respectively. Optimum nitrogen, phosphorus and potassium (NPK) doses for attaining yields of 800 and 1200 kg ha^?1 were derived at soil nitrogen, phosphorus and potassium of 75–400, 10–70 and 150–750 kg ha^?1. Fertilizer nitrogen, phosphorus and potassium ranged from 30–128, 3–19, 13–25 kg ha^?1 and 105–203, 4–32, 27–39 kg ha^?1 for attaining 800 and 1200 kg ha^?1 yield, respectively. The doses could be adopted for attaining sustainable yields under semiarid Alfisols.     

Effect of gypsum on exchangeable sodium percentage and electrical conductivity in the Daeho reclaimed tidal land soil in Korea—a field scale study

Purpose

A reclaimed tidal land along the shore has poor soil properties such as high exchangeable sodium percentage (ESP), and electrical conductivity (EC) due to excess sodium (Na) content. Therefore, Na content should be decreased to improve the land productivity, and for this, gypsum has been widely used. The objective of this study was to determine the changes in ESP and EC of the gypsum-treated reclaimed tidal soil in a field scale.

Materials and methods

For this, gypsum was applied to Daeho reclaimed tidal land (500 ha) in Korea for 5 years (2006 to 2010). The Daeho reclaimed tidal land has been used as reclaimed paddy fields since 1993. The application rate of gypsum was calculated based on exchangeable calcium (Ca) contents and soil cation exchange capacity (CEC) to maintain 60 % exchangeable calcium percentage (ECP) of CEC in soil and the average amount treated was 1570 kg ha^?1 year^?1. The changes in ESP and EC were monitored from 2006 to 2010, and 2013.

Results and discussion

The ESP dropped from 80 % in 2006 to 34 % in 2013. The EC of the soil was decreased by 73 %, from 11.5 dS m^?1 in 2006 to 3.1 dS m^?1 in 2013. Eventually, it was estimated that the ESP will be lowered below 15 % in 2023 with continuous treatment of gypsum according to ECP calculation, and EC will be declined to reach at 0.5 dS m^?1 in 2035, the average EC level of Korean rice paddy.

Conclusions

This field scale study evidenced that gypsum application effectively improves the soil properties of reclaimed tidal soil by decreasing ESP and EC.

     

Effects of biochar and polyacrylamide on decomposition of soil organic matter and <Superscript>14</Superscript>C-labeled alfalfa residues

Purpose

Various soil conditioners, such as biochar (BC) and anionic polyacrylamide (PAM), improve soil fertility and susceptibility to erosion, and may alter microbial accessibility and decomposition of soil organic matter (SOM) and plant residues. To date, no attempts have been made to study the effects of BC in combination with PAM on the decomposition of soil SOM and plant residues. The objective of this study was to evaluate the effects of BC, PAM, and their combination on the decomposition of SOM and alfalfa residues.

Materials and methods

An 80-day incubation experiment was carried out to investigate the effects of oak wood biochar (BC; 10 Mg ha^?1), PAM (80 kg ha^?1), and their combination (BC?+?PAM) on decomposition of SOM and ¹⁴C-labeled alfalfa (Medicago sativa L.) residues by measuring CO₂ efflux, microbial biomass, and specific respiration activity.

Results and discussion

No conditioner exerted a significant effect on SOM decomposition over the 80 days of incubation. PAM increased cumulative CO₂ efflux at 55–80 days of incubation on average of 6.7 % compared to the soil with plant residue. This was confirmed by the increased MBN and MB¹⁴C at 80 days of incubation in PAM-treated soil with plant residue compared to the control. In contrast, BC and BC?+?PAM decreased plant residue decomposition compared to that in PAM-treated soil and the respective control soil during the 80 days. BC and BC?+?PAM decreased MBC in soil at 2 days of incubation indicated that BC suppressed soil microorganisms and, therefore, decreased the decomposition of plant residue.

Conclusions

The addition of oak wood BC alone or in combination with PAM to soil decreased the decomposition of plant residue.

     

Changes in soil bacterial communities in an evergreen broad-leaved forest in east China following 4 years of nitrogen addition

Purpose

Evergreen broad-leaved forest ecosystems are common in east China, where they are both ecologically and economically important. However, nitrogen (N) addition over many years has had a detrimental effect on these ecosystems. The objective of this research was to evaluate the effect of 4 years of N addition on microbial communities in an evergreen broad-leaved forest in southern Anhui, China.

Materials and methods

Allochthonous N in the form of aqueous NH₄NO₃ and phosphorus (P) in the form of Ca(H₂PO₄)₂·H₂O were applied at three doses with a control (CK, stream water only without fertilizer): low-N (50 kg N ha^?1 year^?1), high-N (100 kg N ha^?1 year^?1) and high-N+P (100 kg N ha^?1 year^?1 + 50 kg P ha^?1 year^?1). Quantitative PCR analysis of microbial community size and Illumina platform-based sequencing analysis of the V3-V4 16S rRNA gene region were performed to characterize soil bacterial community abundance, structure, and diversity.

Results and discussion

Bacterial diversity was increased in low-N and high-N treatments and decreased in the high-N+P treatment, but α-diversity indices were not significantly affected by N additions. Proteobacteria, Acidobacteria, and Actinobacteria were the predominant phyla in all treatments, and the relative abundance of different genera varied among treatments. Only soil pH (P = 0.051) showed a weak correlation with the bacterial community in CK and low-N treatment.

Conclusions

The composition of the bacterial community and the abundance of different phyla were significantly altered by N addition. The results of the present study indicate that soil bacterial communities in subtropical evergreen broad-leaved forest are, to a certain extent, resilient to changes derived from N additions.

     

Short-term effects of organo-mineral biochar and organic fertilisers on nitrogen cycling,plant photosynthesis,and nitrogen use efficiency

Purpose

Organo-mineral biochar fertiliser has the potential to replace conventional biochar and organic fertiliser to improve soil quality and increase plant photosynthesis. This study explored mechanisms involved in nitrogen (N) cycling in both soil and ginger plants (Zingiber officinale: Zingiberaceae) following different treatments including organic fertiliser, commercial bamboo biochar fertiliser, and organo-mineral biochar fertiliser.

Materials and methods

Soil received four treatments including (1) commercial organic fertiliser (5 t ha^?1) as the control, (2) commercial bamboo biochar fertiliser (5 t ha^?1), (3) organo-mineral biochar fertiliser at a low rate (3 t ha^?1), and (4) organo-mineral biochar fertiliser at a high rate (7.5 t ha^?1). C and N fractions of soil and plant, and gas exchange measurements were analysed.

Results and discussion

Initially, organo-mineral biochar fertiliser applied at the low rate increased leaf N. Organo-mineral biochar fertiliser applied at the high rate significantly increased N use efficiency (NUE) of the aboveground biomass compared with other treatments and improved photosynthesis compared with the control. There was N fractionation during plant N uptake and assimilation since the ¹⁵N enrichment between the root, leaf, and stem were significantly different from zero; however, treatments did not affect this N fractionation.

Conclusions

Organo-mineral biochar fertiliser has agronomic advantages over inorganic and raw organic (manure-based) N fertiliser because it allows farmer to put high concentrations of nutrients into soil without restricting N availability, N uptake, and plant photosynthesis. We recommend applying the low rate of organo-mineral biochar fertiliser as a substitute for commercial organic fertiliser.

     

<Emphasis Type="Italic">Lotus ornithopodioides</Emphasis> L. a potential annual pasture legume species for Mediterranean dryland farming systems

Twenty-three populations of Lotus ornithopodioides L., collected from different regions of the Mediterranean basin, were investigated for their ecological and agronomic traits in Western Australia. Great variability was found between and within populations for flowering time, forage and seed yield. Flowering time ranged between 75 and 120 days, dry matter production from 2.8 to 4.3 t ha^?1 and seed yield from 284 to 684 kg ha^?1. Other important traits such as non-shattering pods and hard seed were taken into account during the selection to assure an easy seed harvesting and legume persistence in the targeted environments. The high level of hard seed recorded in early winter, associated to the low seedling regeneration, indicates that L. ornithopodioides is best suited to ley cropping systems. Elite lines of L. ornithopodioides characterized by early flowering time, high seed yield and non-shattering pods were selected. Two of them, LOR02.1 and LOR03.2, showed dry matter higher than 4.0 t ha^?1 and seed yield around 700 kg ha^?1 resulting the lines with most potential for Mediterranean farming systems. The results encourage the exploitation of L. ornithopodioides germplasm to develop a new annual self-reseeding legume resource for Mediterranean farming systems for both forage production and crop rotation uses.     

Combined biochar and nitrogen fertilizer reduces soil acidity and promotes nutrient use efficiency by soybean crop

Purpose

Soil acidification is universal in soybean-growing fields. The aim of our research was to evaluate the effects of soil additives (N fertilizers and biochar) on crop performance and soil quality with specific emphasis on ameliorating soil acidity.

Materials and methods

Four nitrogen treatments were applied as follows: no nitrogen (N0), urea (N1), potassium nitrate (N2), and ammonium sulfate (N3), each providing 30 kg N ha^?1. Half plot area of the N1, N2, and N3 treatments was also treated with biochar (19.5 t ha^?1) to form N-biochar treatments (N1C, N2C, N3C). Both bulk and rhizosphere soils were sampled separately for the following analyses: pH, exchangeable base cations (EBC), exchangeable acidity (EA), total inorganic N (IN), total N (TN), and microbial phospholipid fatty acids (PLFAs). Soybean biomass and nutrient contents were also determined. Correlation analysis was applied to analyze the relationships between soil chemical properties and soybean plant parameters.

Results and discussion

With N-biochar additions (N1C, N2C, N3C), soil chemical properties changed as follows: pH increased by 0.6–1.2 units, EBC, IN, and TN increased by 175–419, 38.5–54.7, and 136–452 mg kg^?1, respectively, and PLFAs increased by 23.6–40.9 nmol g^?1 compared to the N0 in the rhizosphere. Microbial PLFAs had positive correlations with soil pH; EBC; exchangeable K, Ca, Na, and Mg; TN; IN; NH₄ ⁺; and NO₃ ^? (r?=?0.66–0.84, p?<?0.01). There were negative correlations between PLFAs and EA or exchangeable Al (r?=??0.64, ?0.66, p?<?0.01), which indicated that the additives increased microbial biomass by providing a suitable environment with less acid stress and more nutrients. The additives increased soil NH₄ ⁺ and NO₃ ^? by promoting soil organic N mineralization and reducing NH₄ ⁺ and NO₃ ^? leaching. Moreover, the soybean seed biomass and the nutrient contents in seeds increased with N-biochar additions, especially in the N3C treatment.

Conclusions

N-biochar additions were effective in ameliorating soil acidity, which improved the microenvironment for more microbial survival. N-biochars influenced N transformations at the plant–soil interface by increasing organic N mineralization, reducing N leaching, and promoting N uptake by soybeans. The soil additive ammonium and biochar (N3C) were best in promoting soybean growth.

     

Ecosystem carbon stock across a chronosequence of spruce plantations established on cutovers of a high-elevation region

Purpose

This study quantified the above- and belowground carbon (C) stocks across a chronosequence of spruce (Picea asperata) plantations established on cutovers and explored the turning point after which the increase in biomass C slowed or biomass C decreased for guiding forest management.

Materials and methods

We assessed above- and belowground plant biomass stocks at 11 sites in three regions, representing 12- to 46-year-old spruce plantations established on clear-cut areas in the eastern Tibetan Plateau, China. Biomass and C stocks of trees, understory vegetation, and forest floor litter were determined from plot-level inventories and destructive sampling. Fine root (<2 mm) biomass and mineral soil organic C (SOC) stock were estimated from soil cores. Tree biomass was quantified using allometric equations based on diameter at breast height (DBH) and height (H).

Results and discussion

Plant biomass C stocks in spruce plantations rapidly increased from 12 to 20 years at a rate of 7.8 Mg C ha^?1 year^?1, but decreased from 25 to 46 years at a rate of 0.79 Mg C ha^?1 year^?1. SOC stocks in spruce plantations gradually decreased from 12 to 46 years at a rate of 4.4 Mg C ha^?1 year^?1. Total C stock in the ecosystem remained unchanged for the first 20 years after the planting of spruce on cutovers, because the buildup of C stock in spruce biomass during the first 20 years was offset by the decrease in SOC. From 21 to 46 years after the reforestation, ecosystem C stock even decreased at a rate of 5.2 Mg C ha^?1 year^?1. The contribution of the understory vegetation, forest floor litter, and fine root to ecosystem C stock was low (<5.0 %) in the spruce plantations.

Conclusions

Ecosystem C stock in the spruce forest established on the cutover in the eastern Tibetan Plateau was related to stand age. During the first 20 years, this ecosystem was C neutral. However, aged (20–46 years) spruce plantation ecosystem can be a C source if no management was implemented to revitalize tree growth, promote understory vegetation, and enhance SOC accumulation.

     

Impact of flue gas desulfurization gypsum and lignite humic acid application on soil organic matter and physical properties of a saline-sodic farmland soil in Eastern China

Purpose

Appropriate land management is important for improving the soil quality and productivity of the saline-sodic farmland. A recent study has revealed that flue gas desulfurization (FGD) gypsum and lignite humic acid application enhanced the salt leaching and crop production. The purpose of this study was to investigate the effects of applied FGD gypsum and lignite humic acid (powder) on the soil organic matter (SOM) content and physical properties.

Materials and methods

This study was based on a field experiment of five consecutive rapeseed-maize rotations in a saline-sodic farmland soil (Aquic Halaquepts) at coastal area of North Jiangsu Province, China. The soil is sandy clay loam texture with pH of 8.43 and clay content of 185 g kg^?1. Six treatments included three FGD gypsum rates (0, 1.6, and 3.2 Mg ha^?1) and two lignite humic acid rates (0 and 1.5 Mg ha^?1). The amendments were incorporated into 0–20 cm soil depth manually every year. Soil samples were collected from each treatment and analyzed for soil organic matter, water-stable aggregates (wet sieving method), bulk density (clod method), water retention capacity (pressure plate apparatus), total porosity (calculated from bulk density and particle density), and microporosity (calculated from water content at 0.01 MPa).

Results and discussion

After 5 years, the SOM and soil physical properties were significantly (P?<?0.05) affected by the application of FGD gypsum and lignite humic acid, especially at the 0–20 cm soil depth. The highest amount of SOM with best soil physical condition was observed in the field which was treated with FGD gypsum at 3.2 Mg ha^?1 with lignite humic acid, and the SOM, total porosity (TP), microporosity (MP), mean weight diameter (MWD), water-stable macroaggregate (WSMA), and available water content (AWC) were increased by 22.8, 6.34, 23.2, 48.1, 55.5, and 15.8 %, respectively, while the bulk density (BD) was decreased by 5.9 % compared to no amendments applied. The generalized linear regression analysis showed that the SOM explained 42.9, 55.0, 48.5, and 54.2 % of the variability for BD, MWD, WSMA, and MP, respectively.

Conclusions

This study illustrates the benefits of applying FGD gypsum and lignite humic acid for increasing the soil organic matter content and improving the soil physical properties and suggests a great potential for ameliorating saline-sodic farmland soil (Aquic Halaquepts) by using combined amendment of FGD gypsum with lignite humic acid.

     

Effect of 13 Years Long Minimum Tillage Cum Conjunctive Nutrient Management Practices on Soil Fertility and Nitrogen Chemical Fractions under Sorghum (Sorghum bicolour (L.) Moench)–Mungbean (Vigna radiata (L.) Wilczek) System in Semi-Arid Tropical Alfisol (SAT) in Southern India

A long-term experiment was conducted at the Central Research Institute for Dryland Agriculture for 13 years to evaluate the effect of low tillage cum cheaper conjunctive nutrient management practices in terms of productivity, soil fertility, and nitrogen chemical pools of soil under sorghum–mung bean system in Alfisol soils. The results of the study clearly revealed that sorghum and mung bean grain yield as influenced by low tillage and conjunctive nutrient management practices varied from 764 to 1792 and 603 to 1008 kg ha^?1 with an average yield of 1458 and 805 kg ha^?1 over a period of 13 years, respectively. Of the tillage practices, conventional tillage (CT) maintained 11.0% higher yields (1534 kg ha^?1) over the minimum tillage (MT) (1382 kg ha^?1) practice. Among the conjunctive nutrient management treatments, the application of 2 t Gliricidia loppings + 20 kg nitrogen (N) through urea to sorghum crop recorded significantly highest grain yield of 1712 kg ha^?1 followed by application of 4 t compost + 20 kg N through urea (1650 kg ha^?1) as well as 40 kg N through urea alone (1594 kg ha^?1). Similar to sorghum, in case of mung bean also, CT exhibited a significant influence on mung bean grain yields (888 kg ha^?1) which was 6.7% higher compared to MT (832 kg ha^?1). Among all the conjunctive nutrient management treatments, 2 t compost + 10 kg N through urea and 2 t compost + 1 t Gliricidia loppings performed significantly well and recorded similar mung bean grain yields of 960 kg ha^?1 followed by 1 t Gliricidia loppings + 10 kg N through urea (930 kg ha^?1). The soil nitrogen chemical fractions (SNCFs) were also found to be significantly influenced by tillage and conjunctive nutrient management treatments. Further, a significant correlation of SNCF with total soil nitrogen was observed. In the correlation study, it was also observed that N fraction dynamically played an important role in enhancing the availability pool of N in soil and significantly influenced the yield of sorghum grain and mung bean.     

System productivity,nutrient use efficiency and apparent nutrient balance in rice-based cropping systems

A 2-year field experiment was conducted to assess system productivity, nutrient use efficiency and apparent balances of phosphorus (P) and potassium (K) in diversified rice-based cropping systems at Gazipur, Bangladesh. Four cropping systems: wheat–fallow–rice, maize–fallow–rice, potato–fallow–rice and mustard–fallow–rice in main plots and four nutrient combinations: NPK, NK, NP and PK in sub-plots were arranged in a split-plot design with three replications. Receiving the NPK treatment, all the component crops gave the highest yield, and omission of N from fertilizer package gave the lowest yield. The maize–rice system removed the highest amount of N (217 kg ha^?1), P (41 kg ha^?1) and K (227 kg ha^?1) followed by wheat–rice, potato–rice and the least in mustard–rice system. The wheat–rice and maize–rice system showed negative K balance of –35.5 and –60.4 kg ha^?1 in NPK treatment, while potato–rice system showed a positive K balance of 31.0 kg ha^–1 with NPK treatment. The N, P and K uptake and apparent recovery by the test crops may be used for site-specific nutrient management. The K rates for fertilizer recommendation in wheat and maize in Indo-Gangetic plain need to be revised to take account for the negative K balance in soil.     

Interactive effects of biochar and polyacrylamide on decomposition of maize rhizodeposits: implications from <Superscript>14</Superscript>C labeling and microbial metabolic quotient

Purpose

The applications of biochar (BC) and polyacrylamide (PAM) may have interactive effects on carbon (C) dynamics and sequestration for improving the soil quality and achieving sustainable agriculture. Relative to BC and PAM, rhizodeposits act as C and energy source for microorganisms and may change the mineralization dynamics of soil organic matter (SOM). No attempt has been made to assess the effects of BC, anionic PAM, or their combination on the decomposition of different aged ¹⁴C-labeled rhizodeposits. The objective of this study was to investigate the effects of the treatments mentioned above on the decomposition of different aged ¹⁴C-labeled maize rhizodeposits.

Materials and methods

biochar (BC) at 10 Mg ha^?1 or anionic PAM at 80 kg ha^?1 or their combination (BC + PAM) was applied to soils with/without 2-, 4-, 8-, and 16-day-aged ¹⁴C-labeled maize rhizodeposits. After that, the soil was incubated at 22 °C for 46 days.

Results and discussion

After 2 days of incubation, the total CO₂ efflux rates from the soil with rhizodeposits were 1.4–1.8 times higher than those from the soil without rhizodeposits. The cumulative ¹⁴CO₂ efflux (32 % of the ¹⁴C input) was maximal for the soil containing 2-day-aged ¹⁴C-labeled rhizodeposits. Consequently, 2-day-aged rhizodeposits were more easily and rapidly decomposed than the older rhizodeposits. However, no differences in the total respired ¹⁴CO₂ from rhizodeposits were observed at the end of the incubation. Incorporation of ¹⁴C into microbial biomass and 66–85 % of the ¹⁴C input remained in the soil after 46 days indicated that neither the age of ¹⁴C-labeled rhizodeposits nor BC, PAM, or BC + PAM changed microbial utilization of rhizodeposits.

Conclusions

Applying BC or BC + PAM to soil exerted only minor effects on the decomposition of rhizodeposits. The contribution of rhizodeposits to CO₂ efflux from soil and MBC depends on their age as young rhizodeposits contain more labile C, which is easily available for microbial uptake and utilization.