Nitrogen uptake of sorghum (Sorghum bicolor L.) from tree mulch and mineral fertilizer under high leaching conditions estimated by nitrogen-15 enrichment

 The effects of applying either inorganic fertilizer or leaf mulch of Acacia saligna (Labill.) H.L. Wend. on yields of Sorghum bicolor (L.) were compared with an unfertilized control under the high leaching conditions of runoff irrigation in a dry tropical environment. The N use efficiency and transfer from ¹⁵N-labelled (NH₄)₂SO₄ or acacia leaves to the sorghum differed in quantity and quality. Only 6% of the applied mulch N was retrieved in the crop, in contrast to 21% of the fertilizer N. The proportions of N in the crop derived from the fertilizers were small, amounting to 7% and 28%, respectively, in the mineral fertilizer and mulch treatments. However, the application of inorganic fertilizer and mulch significantly increased crop grain yield (P<0.05 and P<0.1, respectively), biomass production and foliar N contents (P<0.05). The inorganic fertilizer improved crop yields to a larger extent than mulching. At the same time, more N was lost by applying (NH₄)₂ SO₄ than leaf mulch: only 37% of the N of applied (NH₄)₂ SO₄ was found in the crop and the soil (0–0.3 m), but 99% of the mulched N. High NO₃ ^– contents in the topsoil of the inorganic fertilized sorghum treatments indicated the risk of N leaching. However, more important may have been gaseous N losses of surface-applied NH₄ ⁺. From a nutrient conservation point of view, mulches should be given preferance to inorganic fertilizers under high soil pH and leaching conditions, but larger improvements of crop yields could be achieved with mineral fertilizers. Received: 29 July 1998     

Influence of the presence of nitrite and nitrate in soil on maize biomass production, nitrogen immobilization and nitrogen recovery

 When comparing nitrite (NO₂ ^–) and nitrate (NO₃ ^–) toxicity to maize (Zea mays L.) growth, it is important to know the fate of applied nitrogen (N). A pot experiment, using potassium nitrite (K¹⁵NO₂) and potassium nitrate (K¹⁵NO₃) was conducted to determine the fate of N (0, 75, 150, and 225 mg N kg^–1 soil) applied to a sandy loam soil collected from Gistel (Belgium). The total dry weight of the plants treated with NO₂ ^– was lower than that of the plants treated with NO₃ ^– at 15 and 26 days after N application (harvest 1 and harvest 2, respectively). Shoot and root biomass reduction started at a relatively low NO₂ ^– application rate (75 mg NO₂ ^–-N kg^–1). Biomass reduction increased, at both harvests with increasing amounts of NO₂ ^– to more than 55% at the highest application rate (225 mg NO₃ ^–-N kg^–1). In the NO₃ ^– treatment, a reduction of 16% in total plant dry biomass was recorded only at the highest application rate (225 mg NO₂ ^–-N kg^–1), at both harvest times. The ¹⁵N plant uptake (shoots plus roots) at harvest 1 decreased with increasing N application rates of both N forms (KNO₂ and KNO₃). Twenty-six days after the N application, the total ¹⁵N taken up by the plant increased in all treatments in comparison with 15 days after the N application. However, only at higher rates of N application (150 and 225 mg N kg^–1) was the ¹⁵N uptake by the NO₂ ^– fed plants significantly lower than by the NO₃ ^– fed plants. The percentage of immobilized N from the applied N was low (0–17.7%) at both harvests, irrespective of the N source. However, with relatively low N application rates (75 mg N kg^–1), the immobilized N in the soil decreased with time. This may be due to the re-mineralization of the applied N. The percentage of inorganic ¹⁵N in the soil in NO₂ ^– treatments was slightly lower than in equivalent doses of NO₃ ^–. This might be due to higher losses of N as N-oxides. Unaccounted for N from the applied N ranged from 21% to 52% for the NO₂ ^– treatments and from 3% to 38% for the NO₃ ^– treatments. Received: 17 July 1997     

Effect of selected organic materials and inorganic fertilizer on the soil fertility of a Humic Nitisol in the central highlands of Kenya

The effect on soil fertility of applying particular organic resources to a humic Nitisol in the central highlands of Kenya was studied. The organic resources (Calliandra calothyrsus, Leucaena trichandra, Tithonia diversifolia, Mucuna pruriens, Crotalaria ochroleuca and cattle manure) were either applied solely or along with inorganic fertilizer in a cropping trial using maize as the experimental crop. After 4 years of continuous cultivation and manuring, soil fertility effects varied among treatments. Cattle manure proved to be the most effective and improved soil fertility by increasing pH, cations (Ca, K and Mg), and C. Calliandra, Leucaena, Tithonia and herbaceous legumes generally reduced soil pH, C and N but increased Ca, K and Mg. Cattle manure is therefore an important resource for maintaining soil organic matter (SOM) in the area and in other similar areas with arable‐livestock systems. Reduction of soil C and N by the high quality organic materials suggests that their role in maintaining SOM in the long‐term is limited in this area. A sound nutrient management system should strive to make a balance between maximizing crop production and sustaining soil quality.     

Soil organic matter, microbial biomass and enzyme activities in a tropical agroforestry system

 The effects of growing trees in combination with field crops on soil organic matter, microbial biomass C, basal respiration and dehydrogenase and alkaline phosphatase activities were studied in soils under a 12-year-old Dalbergia sissoo (a N₂-fixing tree) plantation intercropped with a wheat (Triticum aestivum) – cowpea (Vigna sinensis) cropping sequence. The inputs of organic matter through D. sissoo leaf litter increased and crop roots decreased with the increase in tree density. Higher organic C and total N, microbial biomass C, basal soil respiration and activities of dehydrogenase and alkaline phosphatase were observed in treatments with tree-crop combination than in the treatment without trees. Soil organic matter, microbial biomass C and soil enzyme activities increased with the decrease in the spacing of the D. sissoo plantation. The results indicate that adoption of the agroforestry practices led to an improved organic matter status of the soil, which is also reflected in the increased nutrient pool and microbial activities necessary for long-term productivity of the soil. However, tree spacing should be properly maintained to minimize the effects of shading on the intercrops. Received: 21 February 1997     

Urease activity and its relation to soil organic matter, microbial biomass nitrogen and urea-nitrogen assimilation by maize in a Brazilian Oxisol under no-tillage and tillage systems

 We studied the relationship between urease activity (UA) and soil organic matter (SOM), microbial biomass N (N_biom) content, and urea-N fertilizer assimilation by maize in a Dark Red Latosol (Typic Haplustox) cultivated for 9 years under no-tillage (NT), tillage with a disc plough (DP), and tillage with a moldboard plough (MP). Two soil depths were sampled (0–7.5 cm and 7.5–15 cm) at 4 different times during the crop cycle. Urea was applied at four different rates, ranging from 0 to 240 kg N ha^–1. The levels of fertilizer N did not affect the UA, SOM content, and N_biom content. No significant difference between the treatments (NT, DP, and MP) was observed for SOM during the experiment, probably because the major part of the SOM was in recalcitrant pools, since the area was previously cultivated (conventional tillage) for 20 years. The N_biom content explained 97% and 69% of the variation in UA in the upper and deeper soil layer, respectively. UA and biomass N were significantly higher in the NT system compared to the DP and MP systems. The highest maize productivity and urea-N recovery was also observed for the NT system. We observed that the increase in urea-N losses under NT, possibly as a consequence of a higher UA, was compensated for by the increase in N immobilized in the biomass. Received: 2 July 1999     

肥力梯度红壤上不同形态氮库对玉米吸氮量的贡献

不同形态的土壤氮素是作物吸收氮素的主要来源,而土壤肥力不仅影响氮素的含量,也影响氮素的有效性,进而影响作物对氮素的吸收利用。明确不同肥力红壤中各形态氮素的变化及其对作物吸氮量的贡献,可为阐明氮素循环机制和沃土培肥提供理论依据。2019年5月在湖南祁阳红壤实验站选取低肥力、中肥力和高肥力红壤进行田间微区试验,设置不施氮（N0）和常规施氮（N1）两个处理。分析了2020年玉米（该试验的第三季作物）种植前和收获后土壤矿质氮（MN）、固定态铵（FN）、微生物生物量氮（MBN）和可溶性有机氮（SON）含量的变化及其与玉米地上部吸氮量的关系,并通过结构方程模型（SEM）建立了各形态氮库与吸氮量的关系模型。结果发现,N0条件下高肥力土壤的籽粒产量约为中肥力土壤的4.6倍,但在N1条件下,高肥力土壤的玉米产量和生物量与中肥力土壤无显著差异,但其吸氮量显著高于中肥力土壤。与种植前相比,N0条件下,收获后中肥力土壤FN含量显著提高了63%,低肥力和高肥力土壤分别增加了47%和11%。与其相反,土壤MN、MBN和SON含量均有所降低。土壤MN含量降低了0.4~4 mg?kg-1;MBN降低了18%~44%且土壤肥力间无显著差异;SON减少了55%~84%。N1条件下,土壤MN含量降低了约22~38 mg?kg-1; MBN降低了32%~72%;而SON的减少量在高肥力土壤中可达99 mg?kg-1,分别为中肥力土壤和低肥力土壤的2.0倍和9.3倍。相关分析结果表明,地上部吸氮量与MBN、SON和NH4+-N减少量存在显著正相关关系。结构方程模型结果进一步表明,SON和NH4+-N直接影响吸氮量,MBN通过影响SON和MN间接影响玉米地上部吸氮量。总体而言,SON和MBN可直接或间接影响玉米对氮素的吸收利用,是土壤中重要的氮素存在形态,应进一步加强对其形态转化的机制研究,可促进红壤培肥和氮素高效利用。     

Nitrogen and phosphorus uptake by maize as affected by particulate organic matter quality, soil characteristics, and land-use history for soils from the West African moist savanna zone

 The impact of land use (unfertilized continuous maize cropping, unfertilized and fertilized alley cropping with maize, Gliricidia sepium tree fallow, natural fallow) on the soil organic matter (SOM) status and general soil fertility characteristics were investigated for a series of soils representative for the West African moist savanna zone. Three soils from the humid forest zone were also included. In an associated pot experiment, relationships between maize N and P uptake and SOM and general soil characteristics were developed. Soils under natural fallow contained the highest amount of organic C (1.72%), total N (0.158%), and had the highest effective cation exchange capacity (ECEC) [8.9 mEq 100 g^–1 dry soil], while the Olsen P content was highest in the fertilized alley cropping plots (13.7 mg kg^–1) and lowest under natural fallow (6.3 mg kg^–1). The N concentration of the particulate organic matter (POM) was highest in the unfertilized alley cropping plots (2.4%), while the total POM N content was highest under natural fallow (370 mg N kg^–1) and lowest in continuously cropped plots (107 mg N kg^–1). After addition of all nutrients except N, a highly significant linear relationship (R ²=0.91) was observed between the total N uptake in the shoots and roots of 7-week-old maize and the POM N content for the savanna soils. POM in the humid forest soils was presumably protected from decomposition due to its higher silt and clay content. After addition of all nutrients except P, the total maize P uptake was linearly related to the Olsen P content. R ² increased from 0.56 to 0.67 in a multiple linear regression analysis including the Olsen P content and clay content (which explained 11% of the variation in P uptake). Both the SOM status and N availability were shown to be improved in land-use systems with organic matter additions, while only the addition of P fertilizer could improve P availability. Received: 9 April 1999     

Nitrogen mineralization in soil layers, soil particles and macro-organic matter under grassland

 A study was conducted to determine mineralization rates in the field and in different soil layers under three grassland managements (viz. a reseeded sward, a permanent sward with a conventional N management, and a long-term grass sward with 0 N (0-N) input). Potential mineralization rates of soil particles (sand, silt and clay) and macro-organic matter fractions of different sizes (i.e. 0.2–0.5, 0.5–2.0 and >2 mm) were also determined in the laboratory. In the reseeded plots, net mineralization was unchanged down to 40 cm depth. In the undisturbed conventional-N swards, mineralization rates were substantially higher in the top layer (0–10 cm) than in the deeper layers. In plots which had received no fertilizer N, mineralization was consistently low in all the layers. There was more macro-organic matter (MOM) in the 0-N plots (equivalent to 23 g kg^–1 soil for 0–40 cm) than in the two fertilized plots (i.e. conventional-N and reseeded) which contained similar amounts (ca. 15 g kg^–1 soil). C and N contents of separated soil particles did not differ amongst the treatments, but there were large differences with depth. Potential mineralization in the bulk soil was greatest in the 0–10 cm layers and gradually decreased with depth in all the treatments. Separated sand particles had negligible rates of potential mineralization and the clay component had the highest rates in the subsurface layers (10–40 cm). MOMs had high potential rate of mineralization in the surface layer and decreased with soil depth, but there was no clear pattern in the differences between different size fractions. Received: 17 November 1997     

Leucaena hedgerow intercropping and cattle manure application in the Ethiopian highlands III. Nutrient balances

 Balances between nutrients applied or mineralized and nutrients removed in maize grain and stover were calculated in a hedgerow intercropping experiment in which Leucaena leucocephala and L. pallida prunings and cattle manure were applied. Hedgerow intercropping (also called alley cropping) is an agroforestry system in which trees are grown in dense hedges between alleys where short-cycle crops are grown. The hedges are pruned periodically during the cropping period and the prunings are added to the soil as green manure. In control treatments, nutrient depletion per season was in the order of 7–19 kg N ha^–1, 4–12 kg P ha^–1, 10–26 kg K ha^–1, 0–2 kg Ca ha^–1 and 3–6 kg Mg ha^–1. N fertilizer reversed the depletion of N, but it accelerated the depletion of the other nutrients. Manure and at least two applications of leucaena prunings resulted in net positive balances of N, K, and Ca between amounts applied or mineralized and amounts removed by maize. The amounts of P and Mg applied with, or mineralized from, prunings or manure were insufficient to offset the negative balances of these nutrients. Received: 27 January 1998     

Mineralization of nitrogen from decomposing leaves of multipurpose trees as affected by their chemical composition

 Nitrogen release patterns from seven leguminous trees were determined from 8-week laboratory incubations. The quantities of extractable NH₄ ⁺-N and NO₃ ^– released to the soil to which the leaves had been applied was determined at weekly intervals and was related to the initial N, polyphenol, and lignin concentration of the leaves. Cumulative N mineralized was not correlated to initial N, soluble polyphenol and insoluble tannin concentrations, but was correlated to lignin and neutral detergent fibre N (NDF-N) concentrations. The ratios of NDF-N : N (r=0.68 at P<0.05), soluble polyphenol : N (r=0.70 at P<0.05) and (lignin+polyphenol) : N (r=0.75 at P<0.05) were negatively correlated with N release. Total polyphenol content was not a useful predictor of N release, but the reactivity of the polyphenols as measured by their protein-binding capacity can be a useful predictor. In addition to measuring the concentration of polyphenols, their reactivity with proteins must be measured, in order to assess their role in regulating N release. The (lignin+polyphenol) : N ratio could be used to screen leguminous tree leaves for their potential to release N in short-term experiments. This would allow for rapid screening of a large number of trees without detailed and expensive field experimentation. Received: 30 June 1997     

The fate of tree root and pruning nitrogen in a temperate climate alley cropping system determined by tree-injected 15N

 Nitrogen (N) fluxes through the major plant pools of an alder (Alnus sinuta)-sweet corn (Zea mays) alley cropping system were determined over the course of two cropping seasons. Alder trees were injected with ¹⁵NO₃–N to directly follow the flow of N between alder and corn. The contribution of the above- and below-ground tree N to corn was determined by exchanging the labeled above-ground prunings (green manure) with those from unlabeled plots. During the first growing season after coppicing of the injected alders, 18% of the alder ¹⁵N was taken up by the corn with 12% coming from the above-ground prunings. Of the ¹⁵N remaining in the tree/stump following coppicing, the majority was recovered by corn plants within the rows next to the labeled trees during the first growing season. Earlier recovery of ¹⁵N by corn in the labeled root plots compared to the labeled pruning plots indicated the importance of root turnover in supplying N to corn, especially following coppicing. By the end of the first and second growing seasons, 34% and 38% of the ¹⁵N initially present in prunings was recovered in corn plants, respectively. Approximately 80% of the total injected ¹⁵N was found in the soil during the second growing season; however, the turnover of above- and below-ground alder components supplied only 3–4% of the N required by corn during the year of green manure application. Thus, most of the corn N demand was met by mineralization of residual soil N within the 2 years of coppicing and green manure additions. Continued internal cycling of tree N and movement of soil N into more labile pools would presumably allow more alder N to become available over time. The synchronization between N mineralization from the hedgerow green manure components and nutrient uptake of the alley crop remains a major challenge in alley cropping and other green manure systems. Received: 9 April 1999     

Land use affects the distribution of soil inorganic nitrogen in smallholder production systems in Kenya

 We hypothesized that the integration of trees and shrubs in agricultural landscapes can reduce NO₃ ^– leaching and increase utilization of subsoil N. A field survey was conducted on 14 farms on acid soils in the subhumid highlands of Kenya, where there is little use of fertilizers, to determine the effect of vegetation types (VT) on soil NH₄ ⁺ and NO₃ ^– to 4 m depth. The VT included maize (Zea mays) with poor growth and good growth, Markhamia lutea trees scattered in maize, natural weed fallow, banana (Musa spp.), hedgerow, and eucalyptus woodlot. The effect of VT on NH₄ ⁺ was small (<1 mg N kg^–1). NO₃ ^– within a VT was about constant with depth below 0.25 m, but subsoil NO₃ ^– varied greatly among VT. Mean NO₃ ^–-N concentrations at 0.5–4 m depth were low beneath hedgerow and woodlot (<0.2 mg kg^–1), intermediate beneath weed fallow (0.2–0.7 mg kg^–1), banana (0.5–1.0 mg kg^–1) and markhamia (0.5–1.6 mg kg^–1), and high beneath both poor (1.0–2.1 mg kg^–1) and good (1.9–3.1 mg kg^–1) maize. Subsoil NO₃ ^– (0.5–4 m) was agronomically significant after maize harvest with 37 kg N ha^–1 m^–1 depth of subsoil beneath good maize and 27 kg N ha^–1 m^–1 depth beneath poor maize. In contrast, subsoil NO₃ ^– was only 2 kg N ha^–1 m^–1 depth beneath woodlot and hedgerow. These results demonstrate that the integration of perennial vegetation and the rotation of annual and perennial crops can tighten N cycling in agricultural landscapes. Received: 8 July 1999     

Leucaena hedgerow intercropping and cattle manure application in the Ethiopian highlands I. Decomposition and nutrient release

 A litter bag technique was used to study the decomposition and release of N, P, K, Ca, and Mg from Leucaena leucocephala and L. pallida prunings and cattle manure in a hedgerow intercropping trial conducted in the Ethiopian highlands. Hedgerow intercropping (also called alley cropping or alley farming) is an agroforestry system in which trees are grown in dense hedges between alleys where short-cycle crops are grown. The hedges are pruned periodically during the cropping period and the prunings are added to the soil as green manure. Manure was the most resistant to decomposition, losing only 15% of its dry matter (DM) in 15 weeks, compared to 41–57% lost by leucaena prunings. Large quantities of K (up to 104 kg ha^–1) were mineralized from prunings and manure, but Ca and Mg were mostly immobilized. More N and P were released from prunings than from manure, which resulted in net immobilization of these nutrients in the initial stages of decomposition and net mineralization in later stages. Between the leucaenas more N was mineralized and less Ca and Mg were immobilized when L. leucocephala prunings were applied than when L. pallida prunings were applied. Fertilizer N increased DM decomposition and N mineralization. Mineralization of the nutrients was constrained by lignin and polyphenol contents. It is concluded that leucaena mulch and cattle manure may be significant sources of N and K for crop growth, but external sources of P, Ca and Mg may be required, particularly in acid soils which have low contents of these nutrients. However, this fertility effect has to be evaluated against the competition effect of trees to predict crop response. Received: 27 January 1997     

Long-term monitoring of microbial biomass, N mineralisation and enzyme activities of a Chernozem under different tillage management

 We investigated the influence of tillage (conventional, minimum and reduced) on selected soil microbial properties of a fine-sandy loamy Haplic Chernozem over a period of 8 years. The microbial biomass and soil microbial processes were affected mostly by type of tillage and to a lesser extent by the date of soil sampling. Whereas xylanase activity was significantly higher in the 0 to 10-cm soil layer of the reduced and minimum tillage systems within the first year of the experiment (protease and phosphatase activities were significantly higher in the second year), significant treatment effects on microbial biomass, N mineralisation and potential nitrification were observed after a 4-year period. The slow response of substrate-induced respiration to the change in type of tillage may have been due to the differences in the biomass C turnover rates. After a 4-year period, the stratification of the soil microbial biomass within the profile of reduced and minimum tillage systems was probably responsible for the more intensive soil microbial processes near the soil surface compared with conventional tillage. In the 20 to 30-cm layer, N mineralisation, potential nitrification and xylanase activity in the conventional treatment were significantly higher than in the minimum and reduced tillage plots due to buried organic materials. Discriminant analysis underlined the similarity of the enzyme activity patterns in the top layer of the reduced and minimum tillage treatments, and in both layers of the conventional tillage system. The trend towards a significant increase in functional diversity caused by reduced tillage became obvious within the first year of the experiment, and this effect was still manifest after 8 years. All relationships suggested that there were differences in available resources (e.g. organic matter) along the sequence of different tillage systems; this was reflected in part by enhanced enzymatic and microbial activities in the soil layers. In conclusion, this study showed that soils affected by tillage may be classified on the basis of their functional diversity. Therefore, the soil microbial properties chosen for microbiological soil monitoring (microbial biomass, N mineralisation and enzyme activities involved in C, N and P cycling) provide a reliable tool with which to estimate early changes in the dynamics and distribution of soil microbial processes within soil profiles. Received: 3 February 1998     

Transformations and recovery of residue and fertilizer nitrogen-15 in a sandy Lixisol of West Africa

 The fate of ¹⁵N-labeled plant residues from different cover-cropping systems and labeled inorganic N fertilizer in the organic, soil mineral, microbial biomass and soil organic matter (SOM) particle-size fractions was investigated in a sandy Lixisol. Plant residues were from mucuna (legume), lablab (legume), imperata (grass), maize (cereal) and mixtures of mucuna or lablab with imperata or maize, applied as a surface mulch. Inorganic N fertilizer was applied as ¹⁵N-(NH₄)₂SO₄ at two rates (21 and 42 mg N kg^–1 soil). Total N release from mucuna or lablab residues was 2–3 times higher than from the other residues, whereas imperata immobilized N throughout the study period. In contrast, ¹⁵N was mineralized from all the plant residues irrespective of the mineralization–immobilization pattern observed for total N. After 168 days, 69% of soil mineral N in mucuna- or lablab-mulched soils was derived from the added residues, representing 4–8% of residue N, whereas 9–30% of inorganic N was derived from imperata, maize and the mixed residues. At the end of the study, 4–19% of microbial biomass N was derived from the added residue/fertilizer-N, accounting for 1–3% of added residue-N. Averaged across treatments, particulate SOM fractions accounted for less than 1% of the total soil by weight but contained 20% of total soil C and 8% of soil N. Soils amended with mucuna or lablab incorporated more N in the 250–2000 μm SOM pool, whereas soil amended with imperata or the mixed residues incorporated similar proportions of labeled N in the 250–2000 μm and 53–250 μm fractions. In contrast, in soils receiving the maize or inorganic fertilizer-N treatments, higher proportions of labeled N were incorporated into the 53–250 μm than the 250–2000 μm fractions. The relationship between these differences in residue/fertilizer-N partitioning into different SOM particle-size fractions and soil productivity is discussed. Received: 12 March 1999     

The consequences of wheel-induced soil compaction and subsoiling for silage maize on a sandy loam soil in Belgium

In Belgium, growing silage maize in a monoculture often results in increased soil compaction. The aim of our research was to quantify the effects of this soil compaction on the dry matter (DM) yields and the nitrogen use of silage maize (Zea mays L.). On a sandy loam soil of the experimental site of Ghent University (Belgium), silage maize was grown on plots with traditional soil tillage (T), on artificially compacted plots (C) and on subsoiled plots (S). The artificial compaction, induced by multiple wheel-to-wheel passages with a tractor, increased the soil penetration resistance up to more than 1.5 MPa in the zone of 0–35 cm of soil depth. Subsoiling broke an existing plough pan (at 35–45 cm of soil depth). During the growing season, the release of soil mineral nitrogen by mineralisation was substantially lower on the C plots than on the T and S plots. Silage maize plants on the compacted soil were smaller and flowering was delayed. The induced soil compaction caused a DM yield loss of 2.37 Mg ha⁻¹ (−13.2%) and decreased N uptake by 46.2 kg ha⁻¹ (−23.2%) compared to the T plots. Maize plants on compacted soil had a lower, suboptimal nitrogen content. Compared with the traditional soil tillage that avoided heavy compaction, subsoiling offered no significant benefits for the silage maize crop. It was concluded that avoiding heavy soil compaction in silage maize is a major strategy for maintaining crop yields and for enhancing N use efficiency.     

Leucaena hedgerow intercropping and cattle manure application in the Ethiopian highlands II. Maize yields and nutrient uptake

 The effects of Leucaena leucocephala and L. pallida prunings and cattle manure on maize nutrient uptake and yield were investigated in a hedgerow intercropping trial in the Ethiopian highlands. Hedgerow intercropping (also called alley cropping) is an agroforestry system in which trees are grown in dense hedges between alleys where short-cycle crops are grown. The hedges are pruned periodically during the cropping period and the prunings are added to the soil as green manure. For each leucaena species, the experiment had 16 treatments resulting from a factorial combination of four levels of leucaena leaf prunings (no prunings applied; first prunings applied; first and second prunings applied; first, second and third prunings applied), two levels of air-dried cattle manure (0 and 3 t dry matter ha^–1) and two levels of N fertilizer (0 and 40 kg N ha^–1 as urea). Uptake of N, P and K increased significantly with application of the three nutrient sources, but uptake of Ca and Mg either did not respond or decreased with application of prunings and manure. All the three factors increased maize grain and stover yields significantly, usually with no significant interactions between the factors. At least two applications of prunings were required to significantly increase nutrient uptake and maize yield. Maize in the row closest to the hedge did not respond to these nutrient inputs. It is concluded that hedgerow intercropping, with or without manure application, can increase crop yields moderately (to 2–3 t ha^–1 maize grain yields) in the highlands, but P, Ca and Mg may have to be supplied from external sources if they are deficient in the soil. Additional N is still required for higher yields (>4 t ha^–1 maize grain yields). However, quantification of the competition effects of the trees is also required to confirm these results. Received: 27 January 1997     

滴灌水肥一体化条件下施氮量对夏玉米氮素吸收利用及土壤硝态氮含量的影响

河北山前平原夏玉米高产区施肥不合理现象普遍存在,农业面源污染严重。研究华北山前平原水肥一体化条件下夏玉米适宜的氮肥运筹,可为该区氮素优化施用技术及提高氮肥利用效率提供依据。本研究以‘郑单958’玉米品种为材料,于2014—2015年2个玉米生长季,在滴灌条件下设置4个施氮水平(N0:不施氮;N1:120 kg·hm~(-2);N2:240 kg·hm~(-2);N3:360 kg·hm~(-2)),研究滴灌水肥一体化下施氮量对玉米氮素吸收利用和土壤硝态氮含量的影响。结果表明:N0处理的玉米干物质重及产量较其他处理显著降低,N1、N2和N3处理间无显著差异;N1处理的玉米氮含量和氮累积量较N0处理显著增加,施氮量在N1~N3范围内,不同年份间玉米植株氮含量和氮累积量存在一定差异,总体表现为随施氮量的增加而上升的趋势,但随施氮量的增加,植株氮含量和氮累积量上升幅度逐渐降低。N2处理的氮肥收获指数最高。随施氮量增加,氮肥当季回收利用率、氮肥农学效率、氮肥生产效率和氮肥利用效率显著降低;2014年,在0~100 cm土层范围内,4种施氮处理的土壤硝态氮含量均表现为随土层加深逐渐降低;2015年N2和N3处理的土壤硝态氮在80~100 cm土层达到累积峰,经过2年种植后,年施氮量超过240 kg·hm~(-2)的处理,土壤硝态氮淋洗加剧。利用一元二次方程拟合产量与施氮量之间的关系,明确了玉米最高产量的施氮量为199~209 kg·hm~(-2),经济施氮量为174~187 kg·hm~(-2)。综合考虑经济效益和生态效益,该条件下夏玉米滴灌水肥一体化的适宜施氮量为174~187 kg·hm~(-2)。     

Effect of ectomycorrhizae on the growth and uptake and transport of 15N-labeled compounds by Pinus tabulaeformis seedlings under water-stressed conditions

 Seedlings of Chinese pine (Pinus tabulaeformis Carr.) were grown in a growth chamber using a sterilized soil/sand/vermiculite mixture, and were inoculated with the ectomycorrhizal fungus Cenococcum graniforme (Sow.) Ferd. and Winge. The function of external mycelium of ectomycorrhiza for uptake and transport of N from ¹⁵N-labeled NH₄ ⁺ and NO₃ ^– for plant nutrition was evaluated under well-watered conditions and water stress. The pots comprised two-compartments, whereby the penetration of roots was prevented by a nylon mesh bag which the mycorrhizal hyphae were allowed to pass in order to colonize the rest of the pot. ¹⁵N-labeled NH₄ ⁺ or NO₃ ^– was applied to the area of the pot to which the root had no access. At harvest, the ¹⁵NH₄ ⁺ concentration in plant tissues was significantly promoted by the formation of mycorrhizae both under well-watered conditions and water stress. The ¹⁵NO₃ ^– concentration was reduced by water stress and increased by mycorrhizal formation. The enhancement of ¹⁵NO₃ ^– uptake caused by mycorrhizal formation was more evident under water stress than under well-watered conditions. The external mycelia of the ectomycorrhizae took up and transported NH₄ ⁺ and NO₃ ^– from the soil to the plant, thereby improving plant nutrition and growth, in addition to helping the plants to avoid the effects of water stress. Received: 31 October 1997     

Soil microbial biomass carbon and nitrogen as affected by cropping systems

 The impacts of crop rotations and N fertilization on microbial biomass C (C_mic) and N (N_mic) were studied in soils of two long-term field experiments initiated in 1978 at the Northeast Research Center (NERC) and in 1954 at the Clarion-Webster Research Center (CWRC), both in Iowa. Surface soil samples were taken in 1996 and 1997 from plots of corn (Zea mays L.), soybeans (Glycine max (L.) Merr.), oats (Avena sativa L.), or meadow (alfalfa) (Medicago sativa L.) that had received 0 or 180 kg N ha^–1 before corn and an annual application of 20 kg P and 56 kg K ha^–1. The C_mic and N_mic values were determined by the chloroform-fumigation-extraction method and the chloroform-fumigation-incubation method, respectively. The C_mic and N_mic values were significantly affected (P<0.05) by crop rotation and plant cover at time of sampling, but not by N fertilization. In general, the highest C_mic and N_mic contents were found in the multicropping systems (4-year rotations) taken in oats or meadow plots, and the lowest values were found in continuous corn and soybean systems. On average, C_mic made up about 1.0% of the organic C (C_org), and N_mic contributed about 2.4% of the total N (N_tot) in soils at both sites and years of sampling. The C_mic values were significantly correlated with C_org contents (r≥0.41**), whereas the relationship between C_mic and N_tot was significant (r≤0.53***) only for the samples taken in 1996 at the NERC site. The C_mic : N_mic ratios were, on average, 4.3 and 6.4 in 1996, and 7.6 and 11.4 in 1997 at the NERC and CWRC sites, respectively. Crop rotation significantly (P<0.05) affected this ratio only at the NERC site, and N fertilization showed no effect at either site. In general, multicropping systems resulted in greater C_mic : C_org (1.1%) and N_mic : N_tot (2.6%) ratios than monocropping systems (0.8% and 2.1%, respectively). Received: 9 February 1999