中国犬吉氏巴贝斯虫ssrRNA的序列测定及其在PCR诊断上的应用

用特异引物ＢＧ－１和ＢＧ－２从南京株吉氏巴贝斯虫ｃＤＮＡ文库中克隆出ｓｓｒＲＮＡ序列，测定了该序列的核苷酸组成。通过基因库同源性查询表明，该序列与美洲株吉氏巴贝斯虫ｓｓｒＲＮＡ具有８７％的同源性。本实验通过优化ＢＧ－１和ＢＧ－２引物对ｓｓｒＲＮＡ基因的扩增反应条件，建立了对吉氏巴斯虫的特异、敏感的诊断方法。该方法可广泛用于其他血液原虫的ＰＣＲ诊断。     

一例犬吉氏巴贝斯虫病的PCR诊断

1例拉布拉多病犬,食欲废绝,喜卧好喘,小便呈酱油色,血涂片、姬姆萨染色、镜检发现,红细胞内有淡蓝色的环形虫体。采集病犬血液样品,提取基因组DNA,通过PCR方法及对PCR产物进行限制性片段长度多态性分析进而确诊该病犬为犬吉氏巴贝斯虫感染。     

犬吉氏巴贝斯虫病的诊断与治疗

犬巴贝斯虫病是由硬蜱传播而引起的血源性原虫病,目前本病在宠物犬中经常发生,危害日益严重.该病有一定的发病规律,患犬以严重的贫血和血红蛋白尿为特征.严重感染时可引起贫血、呕吐、腹泻.本文介绍了两个病例,根据病犬的临床症状,通过实验室的检查,诊断出此犬为吉氏巴贝斯虫病,并给予了相应的药物治疗,经治疗后两犬的病情逐渐好转,最...     

一例犬吉氏巴贝斯虫病的诊治

3岁雄性贵宾犬,体重3.20 kg,免疫完全,未绝育,从未进行过体内外驱虫,院内散养。主诉最近2周发现犬的精神状态渐差,进食量逐渐减少。为了对其进行诊治,采用体格检查、血液学检查以及影像学检查的方法进行诊断,并根据诊断结果进行治疗,结果表明,该犬感染吉氏巴贝斯虫且并发肝脏损伤,通过采取输血、驱虫等治疗措施,患犬的临床症状消失,镜检外周血虫体消失,治疗后未见复发。说明临床上可通过血液学检测对犬的吉氏巴贝斯虫感染进行确诊,且输血结合驱虫治疗可有效治疗该病。     

犬吉氏巴贝斯虫病的诊断与治疗

河北省秦皇岛某犬养殖中心的50只犬出现红尿、厌食、发烧、精神欠佳等症状,通过流行病学调查、临床症状观察、实验室检查,在其血涂片检查中发现红细胞内有染成淡蓝色的小梨籽形虫体,有圆形、环形和逗点形等,虫体的染色质团呈紫红色,清晰可见,1个红细胞内寄生虫体1~3个不等,红细胞染虫率为5%~15%,确诊为犬吉氏巴贝斯虫病。应用伯氨喹啉(0.8~1.0 mg/kg)和乙胺嘧啶(0.8 mg/kg)配合治疗,连用5 d。用药后贫血症状基本缓解,食欲恢复,红细胞染虫率下降到了1%左右,病犬痊愈。表明伯氨喹啉和乙胺嘧啶对犬吉氏巴贝斯虫病具有良好的防治效果。     

一例犬吉氏巴贝斯虫病的诊断与治疗

文章介绍了1例犬吉氏巴贝斯虫病的诊治情况。通过询问病史、临床检查、血液学检查和聚合酶链式反应（PCR）进行综合分析诊断,采用对症、支持及输血疗法治疗,预后良好。     

吉氏巴贝斯虫的冷冻试验

吉氏巴贝斯虫(Babesia gibsoni)是寄生于犬体内的血液原虫,该虫经中间宿主蜱传播,对我国部分地区的军、警犬及猎犬的危害严重。目前,对该虫的实验室保种方法主要有二种,一种是保存带虫阳性蜱,另一种是将虫体保存在犬体内。这两种方法都比较繁琐,且不经济。如今,牛巴贝斯虫的冷冻保存方法已经建立。但吉氏巴贝斯虫的冷冻保存方法还未见报道。本实验以二甲基亚砜(DMSO)和甘油作为冷冻保护剂,对该虫进行了冷冻保存试验,现将结果报道如下。     

36例犬吉氏巴贝斯虫病的诊断与治疗

西安市某宠物医院从2018年-2019年共接诊了36例犬吉氏巴贝斯虫病,对这36例病例的临床症状、流行病学、实验室诊断和治疗进行了调查和研究.结果表明,犬感染吉氏巴贝斯虫后主要症状为虚弱、发热、尿黄等.该病的高发期在9月和10月,占总发病的50％.发病年龄主要是1岁至5岁犬,占69.4％.实验室检查显示感染犬的红细胞和...     

北京地区25例犬吉氏巴贝斯虫病例分析

为调查北京地区犬吉氏巴贝斯虫病的感染与治疗情况,2014年10月至2016年1月期间,本试验对中国农业大学动物医院就诊的25例犬吉氏巴贝斯虫感染病例进行调查统计,记录其临床症状,分析全血细胞计数(Complete Blood Count,CBC)、生化检测结果和三氮脒的治疗效果。结果显示,25例患犬均出现不同程度的贫血,73.3%(11/15)的患犬出现了免疫介导性溶血性贫血(Immune mediated hemolytic anemia,IMHA),使用三氮脒和输血疗法治疗后,71.2%(10/14)的患犬取得良好的疗效,但目前跟踪的病例中,21.4%(3/14)的患犬已复发。     

犬吉氏巴贝斯虫截短型抗原BgTRAP的重组表达及其在诊断上的初步应用

吉氏巴贝斯虫Bg TRAP蛋白是一个重要的诊断抗原候选分子。为建立一种实用的犬吉氏巴贝斯虫血清学诊断方法,本研究选取Bg TRAP C-末端跨膜区前,包含TSP功能区和抗原区的411个氨基酸的编码基因片段,重组表达了一个可溶性截短型Bg TRAP抗原,解决了完整蛋白重组表达纯化的困难。免疫荧光试验表明,截短型抗原具有良好的抗原性。纯化的截短型抗原作为酶联免疫试验诊断抗原,可清晰地区分阴性及阳性犬血清,并与其他病原感染无交叉反应,具有良好的特异性。犬感染吉氏巴贝斯虫系列血清检测表明,该抗原可检测早期感染(4 d)和感染200 d以后的样本。结果提示,重组Bg TRAP截短型抗原可作为一种诊断制剂检测犬吉氏巴贝斯虫抗体。     

Babesia gibsoni infection in a dog from indiana

Abstract: A 10-year-old spayed female mixed-breed dog was presented to the Purdue University Veterinary Teaching Hospital (PUVTH) with complaints of persistent anemia with occasional exacerbations, anorexia, and lethargy. The dog had been presented to the referring veterinarian 2 months prior with multiple bite wounds received during a fight with 3 Pit Bull Terriers. The dog was discharged after the wounds were cleaned and surgically closed. Upon admission to the PUVTH, blood was collected for a complete blood count and biochemical analysis. Microscopic examination of peripheral blood smears revealed intraerythrocytic protozoal parasites consistent with Babesia gibsoni . Molecular analysis confirmed that the organism was B gibsoni and that its 18S ribosomal RNA sequence was identical to that of other B gibsoni isolates from Oklahoma, North Carolina, and Okinawa, Japan. Hematologic changes included moderately severe, regenerative, macrocytic, normochromic anemia, with poikilocytosis, polychromasia, anisocytosis, and a marked increase in nucleated RBCs. Biochemical changes included increased serum alanine aminotransferase, alkaline phosphatase, and gamma glutamyl-transferase activities. The dog was treated with imidocarb, but despite initial clinical improvement, the dog died 2 weeks after the first dose. A necropsy was not performed. The infection in this dog is the first reported case of B gibsoni infection in Indiana. Because of the widespread geographical distribution of the organism, veterinarians and veterinary clinical pathologists throughout the United States should carefully examine Romanowsky-stained blood smears from patients with acute hemolytic anemia for small intraerythrocytic babesial parasites.     

Babesia gibsoni infections in dogs from North Carolina

The recognition of canine babesiosis in North Carolina caused by Babesia gibsoni documents the expansion of the previously reported endemic area of this disease. Clinical signs ranged from severe hemolytic anemia and thrombocytopenia to subclinical infections. No infected dogs had traveled to endemic areas. Antibabesial treatment failed to eradicate the organism from infected dogs.     

间接荧光抗体试验诊断犬吉氏巴贝斯虫病

利用冷藏抗原玻片 ,用间接荧光抗体试验检测感染吉氏巴贝斯虫犬血清 ,具有特异性强、敏感性高、快速、准确、操作简单等特点。对疫区 82份犬血清的检查 ,阳性率为 37 8% ;对非疫区 50份犬血清的检查 ,假阳性率为 2 %。与血液涂片染色检查比较 ,该法检出率高 ,结果可靠 ,可用于犬吉氏巴贝斯虫病的诊断和流行病学调查     

一例犬吉氏巴贝斯虫感染引起的免疫介导溶血性贫血的诊治

犬免疫介导溶血性贫血是一种与自身红细胞抗体有关的溶血性贫血,发病原因可分为原发性和继发性,论文主要介绍了一例由吉氏巴贝斯虫感染后继发的犬自身免疫溶血性贫血的诊断、治疗和预后。     

猪源致病性沙门氏菌四环素耐药基因tetC的PCR扩增及序列分析

四环素类抗生素作为一类广谱抗生素,现已产生严重的耐药性.作为人兽共患病原菌的沙门氏菌,也存在大量这样的耐药菌株,使得本病的发病率和死亡率都不断上升.本研究对规模化猪场分离的经动物试验和生化试验鉴定的致病性沙门氏菌16株、药敏质控菌ATCC25922和沙门氏菌标准株C79-13对进行了四环素、金霉素、土霉素的药敏试验,结果表明16株致病性沙门氏菌对四环素类抗生素表现出普遍耐受性,耐药率达100%.其中MIC值＞128μg/mL的高耐药菌株13株,高耐药率81.25%;MIC值为32μg/mL的低耐药菌株3株,低耐药率18.75%.设计沙门氏菌四环素抗性基因tetC的引物,对16株致病性沙门氏菌的tetC基因做PCR扩增,结果12株菌获得以质粒为模板长约400bp的特异性扩增产物,未能从染色体扩增到产物.分别对临床分离的1株低耐药菌株(DY1)和1株高耐药菌株(SL1-3)的tetC基因扩增产物进行序列分析,结果表明菌株DY1和SL1-3的tetC的核苷酸同源率为97.7%;菌株DY1与质粒PBR322中tetC的核苷酸同源率为98.7%;菌株SL1-3与质粒pBR322中tetC的核苷酸同源率为98.4%,说明对于耐药程度和地方来源各不相同的菌株,在核苷酸序列上同源率很高.本文用PCR技术对规模化猪场分离的致病性沙门氏菌的四环素耐药基因进行了研究,以期对四环素耐药性的分子流行病学进行监测,克服了药敏试验只能检测耐药表型的缺点,为有效控制沙门氏菌感染提供理论基础和科学依据,在兽医、食品卫生和公共卫生等方面具有重要的意义.     

Transfusion-associated Babesia gibsoni infection in a dog

A 2.5-year-old spayed female German Shepherd Dog was referred for evaluation of progressive anemia, lethargy, and weight loss. Seventeen days earlier, the dog had received a whole blood transfusion to manage hemorrhage after ovariohysterectomy. Mild fever, splenomegaly, and thrombocytopenia were also identified. Von Willebrand disease and Babesia gibsoni infection were diagnosed. Because of the serologic cross-reactivity of B gibsoni and B canis in the immunofluorescent antibody assay for IgG antibodies against these organisms, polymerase chain reaction amplification of parasite DNA was required to identify the infecting Babesia sp. The source of the B gibsoni infection was traced to an apparently healthy American Pit Bull Terrier blood donor. Despite resolution of clinical signs in the dog of this report, a series of antiparasitic treatments failed to eliminate the B gibsoni infection. Screening of potential blood donor dogs for Babesia spp is becoming increasingly important in the United States.