Untersuchungen zur Assimilatverteilung in Ackerbohnen (Vicia faba L.) während der Blühphase

Investigations about the distribution of assimilates during flowering in broad beans ( Vicia faba L.)
The distribution of assimilates during flowering was studied on single plants exposed to ¹⁴CO₂ in the field. The plants were harvested 1, 3, 15 days after exposition and at ripening. Results:
1. At the begin of flowering the nodes with flowers assimilated much ¹⁴C with a tendency of higher contents in the more above situated nodes. The concentration of ¹⁴C was similar in blades, stalks and flowers.
2. The assimilates incorporated after 24 hours were only to a small amount translocated afterwards. Only 3 % went to the apical region. An intensive restorement out of the blades took place at the time of ripening.
3. In the midst of flowering the concentration of ¹⁴C was lower in the nodes with open flowers than in those with shut or with pods. At that time pods are already strong sinks that withdraw assimilates from blades and stalks.
4. At the time when large and small pods are growing on the lower and middle nodes, the nodes in the apical region will be deprived of assimilates (effects of dominance).
5. Roots and nodules had low but stable contents and concentrations of ¹⁴C during flowering. These descended distinctly at ripening.     

Heterosis and hybrid performance in topless faba beans (Vicia faba L.)

Parental lines, F₁ and F₂ hybrids from a 7×7-diallel of topless faba beans and eight standard cultivars were grown in single-rows for two years at four sowing dates on a highly fertile loam soil near Göttingen. In grain yield, F₁ hybrids showed 21 to 54% advantage over the higher yielding parent. Compared with the midparental value an average heterosis of 50% was observed for grain yield. Corresponding heterosis mean values ranged from 3 to 37% for yield components and up to 11% for phenological traits. The grain yield of the highest yielding topless F₁ hybrid was equal to that of Alfred, the highest yielding indeterminate standard cultivar. High grain yield in the topless hybrids was closely correlated with high biomass yield, late ripening and tall plants generally having a high number of podded nodes and pods on the main stem. Due to their agronomic advantages, i.e., lower plant length, reduced lodging sensitivity and earlier ripening, efforts at breeding topless hybrids seemed to be worth-while in faba beans. However, their yielding ability must be improved further before they can compete with normal, semi-dwarf and stiff-strawed cultivars.     

Untersuchungen zur Nutzbarkeit des generativen Speicherpotentials von indeterminierten Ackerbohnengenotypen (Vicia faba L.)

Investigations on the utilization of the generative storage potential of indeterminated field bean genotypes ( Vicia faba L.)
The gradation in seed yield between and within the inflorescences of a field bean plant results from a hierarchical control by way of internal competition in connection with hormonal regulation. Following this hypothesis in genotypes with different yielding capacity (2 inbred lines, 1 hybrid) the two proximal flowers/inflorescence of the nodes 3–6 (counted from the bottom of the plant) were either removed or treated with growth regulators. AMO-1618 was applied for inhibition of the gibberellic acid biosynthesis, or chlorflurenolmethylester (CFM) for reduction of indole-3yl acetic acid translocation as both phytohormones might be concerned with the hierarchical control of the storage system. — Independent from yield level a reduction of the used storage potential in control plants up to 50% by treatments was fully compensated for in other regions and positions of the plant. AMO-1618 application in some cases indicated an increased seed yield/plant, whereas CFM only increased the number of sterile pods/plant. These effects are discussed in relation to the storing efficiency and to yield stability of an indeterminated faba bean plant.     

Inheritance and linkage of a gene for testa-imposed seed dormancy in faba bean (Vicia faba L.)

Seed dormancy in the grain legumes may interfere with germination in some situations, yet is not well described. Dormancy in faba bean (Vicia faba L.) was investigated to determine the mode of inheritance and whether the gene or genes controlling this trait are linked to other genes. Seeds produced by 235 recombinant inbred lines at the F₆ generation were used for this study. The parents were the cultivar ‘Optica’ and 172, an Afghanistan landrace which possesses extended seed dormancy. Germination was scored on a seed sample from each recombinant inbred line. Segregation patterns indicated that the trait is monogenic. This gene, named doz, is linked to a gene controlling anthocyanin and proanthocyanidin synthesis, sp-b, with the genetic distance estimated to be about 25 cM. No associations were detected between seed dormancy and pod dehiscence, seed lustre or mean seed weight. The implications of the persistence of a gene for seed dormancy in the gene pool of V. faba for the introgression of traits from landraces and cultivars possessing dormancy and for the origins of domesticated V. faba are discussed.     

Rate and inheritance of cross-fertilization in faba bean (Vicia faba L.)

Summary To assess the feasibility for synthetic varieties of Vicia faba L., 108 faba bean genotypes were screened for cross-fertilization rate in five field experiments carried out in 1989 and 1990 at Wageningen.Significant genotypic differences were observed for outcrossing rate, which ranged from 1 to 55%. These percentages, however, are thought to be not high enough for a commercially feasible production of synthetic varieties.The inheritance of cross-fertilization rate, determined by testing the F₄ generation of a cross between two inbreds, both with high outcrossing rates, appeared to be of a polygenic nature, as was indicated by the wide variation in, and the continous distribution of, the cross-fertilization rates. Among the F₄ lines tested some had a significantly higher cross-fertilization rate than the parents. However, this difference was not found when these lines were retested, indicating that possibilities for successful selection for higher outcrossing rates might be limited.     

Differential Drought Responses of Faba Bean (Vicia faba L.) Inbred Lines

Drought responses of 19 inbred faba bean lines of different origin were studied in the field under rain shelters with and without irrigation. Inbred lines differed significantly in response to drought (P < 0.01): those with a lower drought sensitivity index (SI) (more resistant) originated from the drought-prone regions characterized by smaller plant size (r = 0.93), and more pods and seeds per plant (r ≥ 0.90) regardless of seed size, while lines with higher SI (more sensitive) were those which mainly exhibited higher yield under favourable conditions accompanied by a greater biomass. In a greenhouse experiment under mild drought (−0.15 MPa soil water potential), comparisons between relatively drought-sensitive (Adriewaalse) and drought-resistant (L7) inbred lines showed that Adriewaalse used 38 % more water than L7 and also produced 40 % more biomass. There was a stress-induced decrease in osmotic potential (ψ_s) in both lines (by 0.72 and 0.50 MPa for Adriewaalse and L7, respectively) accompanied by decreased turgor in Adriewaalse and increased turgor in L7. The difference in drought-induced solute accumulation between lines was diminished when solute accumulation due to water loss and growth inhibition was considered, which indicates that solute accumulation was the result of a concentration effect. Similarly, lower SI in the field was not the result of osmotic adjustment, as the relationship between SI and drought-induced decrease in ψ_s was negative. The water use efficiency of both inbred lines increased markedly with increasing water deficit, though there was no difference between the lines. It was concluded that differences in drought resistance between these inbred faba bean lines were manifested through plant size-induced water demand (avoidance) but were not associated with osmotic adjustment (tolerance).     

The identification of faba bean (Vicia faba L.) trisomics by translocation tester set

Summary Five subtelocentric primary trisomics of Vicia faba L. were isolated from crosses between asynaptic mutants and normal plants or translocation tester stocks. The extra chromosome of each trisomic was identified cytologically by critical multivalent association at metaphase I of meiosis.     

Yield Stability in Faba Bean,Vicia faba L. 1. Variation Among Inbred Lines*

Cultivation of faba beans (Vicia faba L.) is hampered by poor yield stability. The genetic variation at the homozygous level for yield stability in the gene pool of the small-seeded and indeterminate European faba beans and the usefulness of auxiliary traits for the improvement of yield stability were investigated. The concept of stability, based on the regression technique, was applied. A sample of 36 faba bean lines was tested in 16 environments and a subsample of eight lines was tested in 11 additional environments. Significant differences were found between lines for yield stability parameters, but the repeatability of the results was limited. Early maturity correlated markedly (r = 0.51^**) with one of the yield stability parameters (deviation from regression). Although lodging resistance was not correlated with the stability parameters, it proved to be a safety factor for performance.     

Interspecific hybridization between Vicia faba (L.) and Vicia narbonensis (L.): Early pod growth and embryo-sac development

Summary Fertilized embryo-sac development and pod growth was studied in one Vicia faba cultivar, one Vicia narbonensis population and their reciprocal crosses. The initial development of endosperm and embryo was at least four days faster in V. narbonensis than in V. faba. Pods and ovules developed also faster in V. narbonensis than in V. faba. The growth rate of the hybrid pods followed the growth rate of the mother species, but was slower than that of the pods from selfed flowers. In the cross V. narbonensis × V. faba the ovules stopped growing 9 days after pollination, while in the reciprocal cross they stopped growing 15 days after pollination. Hybrid embryo-sacs from V. faba × V. narbonensis were aborted before they reached the stage of 256 endosperm nuclei or 200 embryo cells. Selfed V. faba embryo-sacs reached this stage in less than 9 days after pollination. In the reciprocal cross the embryo-sacs were aborted before they reached the stage of 128 endosperm nuclei or 80 embryo cells. Selfed V. narbonensis embryo-sacs reached this stage at the 4th day after pollination. Given that at these stages the embryo has less than 200 cells it was concluded that an in-ovule embryo culture technique should be developed to obtain hybrid plants.     

Intraspecific Variation in Mean Endosperm Cell Cycle Time in Vicia faba (L.) and Interspecific Hybridization with Vicia narbonensis (L.)

The rate of early embryo-sac development was studied in seven faba bean (Vicia faba L.) cultivars grown in a controlled environment. The embryo-sac stage was determined from the number of endosperm nuclei per embryo-sac and the number of embryo cells during the first 12 days after pollination. Differences in early embryo-sac development were observed between the genotypes studied. In addition, five different V. faba×V. narbonensis crosses and reciprocals were made using genotypes with small differences in mean endosperm cell cycle time and genotypes with large differences. The percentage of success (pod set) in V. faba×V. narbonensis crosses ranged between 9 % and 59 % while in the reciprocals it ranged from 12 % up to 30 %. However, cytological studies showed that the high percentage of success (pod set) observed in the cross A-107 × A-202 was not associated with a higher percentage of interspecific hybrid embryos. The results indicate that genotypes of the two species with smaller differences in mean endosperm cell cycle time result neither in a higher percentage pod set nor in bigger hybrid embryos.     

Effectiveness of early generation selection under two plant densities in faba bean (Vicia faba L.)

The effectiveness of early generation selection under low and high plant density was evaluated in faba bean (Vicia faba L.). For this, the faba bean cv. 'Polycarpe' was pollinated by eight higher yielding cultivars of different origin. The F₁'s and F₂'s together with 'Polycarpe' and the pollinator with the highest yielding ability (cv. 'Effe') were evaluated under high plant density with adjacent controls and under low plant density in a honeycomb design. It was found that the eight hybrids had a different ranking at low than at high plant density for both generations (F₁and F₂). Hybrid Pol.x A-58 had the best combined yield performance in the F₁and F₂under high and hybrid Pol.x A-37 under low plant density. Application of individual plant selection in the F₂and F₃generation of the two crosses (Pol.x A-58 and Pol.x A-37), under both low plant density according to UNR-O honeycomb design and high plant density using a moving average, resulted in the selection of four groups with 20 selected F₃plants each. Within each group, equal numbers of seeds from each plant were mixed to establish four mixtures. These mixtures together with two controls ('Polycarpe' and 'Effe') were evaluated in a randomized complete block design (22 plants/m²) in one location for two years. It was found that the material selected continuously under low plant density had a significantly higher yield than the material selected under high plant density. In addition, the material selected from Pol.x A-37 (H₁R₂) under high plant density had a lower mean yield than that selected continuously under low plant density (H₁H₂). Their average yield over years was significantly higher than the yield of the material selected from Pol.x A-58 under both plant densities. Furthermore, the material selected from Pol.x A-58 under low plant density, had a significantly higher mean yield over years than its sibs selected under high plant density. It was concluded that honeycomb selection at low plant density can be used effectively for early generation selection in faba bean.     

Stomatal and Non-stomatal Effects of Salinity on Photosynthesis in Faba Beans (Vicia faba L.)

The effect of salinity (40 and 80 meq/1 NaCl) on stomatal conductance, chlorophyll content, ultrastructural damage and photosynthetic performance of six cultivars of faba beans ( Vicia faba L.) from different agroclimatic regions were investigated. Low levels of salinity (40 meq/1 NaCl) increased chlorophyll content in some cultivars but did not lead to simultaneous increase in photosynthesis (P). Increased salinity levels (80 meq/1 NaCl) decreased chlorophyll content and stomatal conductance. Ultrastructural damage was observed in sensitive cultivars even at low salinity levels. Based on the parameters investigated, there was no concrete evidence to suggest that the physiological processes of cultivars from arid and semi-arid regions are less affected under saline conditions as compared to cultivars from temperate regions. The results suggested increased salinity affects P not only due to its effect on stomatal closure but also due to other non-stomatal effects like ultrastructural damage and decrease in chlorophyll content below a threshold level.     

Autofertility and Bee Visitation in Winter and Spring Genotypes of Faba Beans (Vicia faba L.)

Differences in the expression of autofertility and in the evidence of attractiveness to bees were investigated in both autumn-sown and spring-sown faba beans. All six winter stocks and one spring stock expressed very little autofertility, with averages of only 10% of flowers and 7% of ovules fertilized when undisturbed. Three other spring stocks, including a closed-flower population, were moderately autofertile, with 22% of flowers and 17% of ovules fertilized when undisturbed. The remaining two spring stocks, both English cultivars, expressed good autofertility, with 50% of undisturbed flowers and 44% of ovules fertilized. Current levels of autofertility in the winter and closed-flower stocks were inadequate to allow the development of a desirable distribution of pods without substantial bee visitation. If levels of autofertility could be raised simply by selection within populations, then they should have been much higher in these stocks. Therefore, autofertile parents will probably have to be included in programmes for breeding cultivars with improved levels of auto fertility. The closed-flower population received significantly less bee visitation than the other stocks in the spring trial. No variation in attractiveness to bees was found among the open-flowered spring stocks, nor among the winter stocks.     

Susceptibility of Faba Bean (Vicia faba L.) to Heat Stress During Floral Development and Anthesis

Experiments were conducted over 2 years to quantify the response of faba bean (Vicia faba L.) to heat stress. Potted winter faba bean plants (cv. Wizard) were exposed to temperature treatments (18/10; 22/14; 26/18; 30/22; 34/26 °C day/night) for 5 days during floral development and anthesis. Developmental stages of all flowers were scored prior to stress, plants were grown in exclusion from insect pollinators to prevent pollen movement between flowers, and yield was harvested at an individual pod scale, enabling effects of heat stress to be investigated at a high resolution. Susceptibility to stress differed between floral stages; flowers were most affected during initial green‐bud stages. Yield and pollen germination of flowers present before stress showed threshold relationships to stress, with lethal temperatures (t₅₀) ?28 °C and ~32 °C, while whole plant yield showed a linear negative relationship to stress with high plasticity in yield allocation, such that yield lost at lower nodes was partially compensated at higher nodal positions. Faba bean has many beneficial attributes for sustainable modern cropping systems but these results suggest that yield will be limited by projected climate change, necessitating the development of heat tolerant cultivars, or improved resilience by other mechanisms such as earlier flowering times.     

The Influence of Day Length on Plant and Nodule Development of Fababeans (Vicia faba L.) and Grasspeas (Lathyrus sativus L.)

The valuation of the influence of different day length (DL) on plant and especially nodule development of fababeans ( Vicia faba L.) and grasspeas ( Lathyrus sativus L.) was the aim of these experiments. For that, experiments in growth chambers (Exp. 1) with DL of 10, 13, 16 and 19 hours and an experiment in vegetation boxes (Exp. 2) with DL of 7, 10, 13 hours and natural DL as control (CON) were conducted. The development of plants was studied by 6 and 7 harvests (HA) respectively beginning at 27 and 31 days after emergence (DAE) respectively, as well as consecutive determinations of leaf area, dry matter (DM) of the different plant organs and nodule number.
Total- and pod-DM of both species increased with higher DL (19 h, CON). Nodule-DM was positive influenced by longer assimilation duration and bigger leaf areas of the higher DL treatments. With onset of pod ripening, which occurred early under higher DL, lower nodule-DM was determined due to the beginning of nodule senescence. Under 19 h DL the fababeans established highest nodule-DM, 1,100 mg/plant, whereas the grasspea 410 mg only. Nodules under shorter DL (7 h, 10 h) showed less intensive red tissue and less nodules > 6 mm diameter. The grasspea established more bigger nodules than the fababeans. Total nodule number was not unequivocal affected by DL, however, it increased with the course of vegetation. Senescence of nodules was most evident at time of highest growth rates of the pod-DM.     

N2-Fixierung und Ertragsstruktur der Weißen Lupine (Lupinus albus L.) im Vergleich zu Vicia faba L. und Glycine max (L.) Merr. auf verschiedenen Standorten

N₂-Fixation and Yield Structure of White Lupin ( Lupinus albus L.) in Comparison to Vicia faba L. and Glycine max (L.) Merr. on Different Sites
In field studies white lupin ( Lupinus albus L., Eldo ) was compared with faba bean ( Vicia faba L., Herz Freya ) in 1986 and soybean ( Glycine max [L.] Merr., Gambit ) in 1988 on five sites respectively. Total N₂-fixation, which was determined by the extended difference method, and yield components were correlated to weather and soil conditions:
1. While faba bean responded to low pH with delayed nodulation, white lupin showed no decrease in N₂-fixation at pH less than 5.5 as far as soil was not calcareous.
2. The white lupin developed its root system most quickly into further soil depths and produced a root dry weight six times as large as that of faba bean in the soil layer 60-90 cm until the end of July (Bayreuth).
3. Despite on the calcareous sites the white lupin showed the highest total-N₂-fixation (max. 36 g N/m²) throughout, the N-gain for the succeeding crop was up to 8 g N/m² for white lupin and faba bean as well. In contrast the N-balance of soybean was mostly negative.
4. Seed yields of white lupin (48-450 g/m²) ranged between those of faba bean (145-549 g/m²) and of soybean (89-290 g/m²); its raw protein yields were the highest found (max. 158 g/m²) though.     

蚕豆异交率相关因素研究及应用进展

蚕豆是常异花授粉作物,异交率一般为20%~30%,最高可达84%。媒介昆虫、气候条件、地理因素、种植密度、品种基因型、花序的小花数量及小花结构特点、田间环境等都对异交率产生影响,媒介昆虫是异交发生的主要途径。异交会导致种质一致性的丧失,给资源的保存利用、品种繁育和应用基础研究等带来一定阻碍,但同时异交又是群体遗传多样性保障和杂种优势利用的重要条件。本文从异交率、异交媒介、异交影响因素及异交率的应用研究4个方面总结蚕豆异交率研究和应用情况,并对今后研究方向进行展望。     

Pattern analysis and genotypic x environment interactions in faba bean (Vicia faba L.) populations

Summary Twenty-nine populations of faba bean (Vicia faba L. subsp. major, equina and minor) of mediterranean origin were evaluated for the genetic variability of 12 biomorphological characters at four locations in Apulia and Basîli cata regions (Italy) in 1983–84.A pattern analysis appoach, based on classification and ordination procedures is presented for to characterize differences among population in mean performance and response across environments. Informations about G × E interaction were obtained.Research work supported by CNR, Italy. Special grant I.P.R.A. Sub-project 1. Paper N. 1099.     

Dry Peas (Pisum sativum L.) Grown in Mixtures with Faba Beans (Vicia faba L.)—A Rewarding Cultivation Alternative

Two semi-leafless pea cultivars and two highly lodging-resistant faba bean cultivars were grown in a series of replacement trials conducted on a highly fertile loam soil near Gottingen from 1992 to 1995. Cultivation as well as harvesting of mixed crops of both species proved possible. In spite of the delayed harvest of the mixed crop stands determined by the later pod ripening of the faba beans (varying between seasons from -f 5 to +21 days), noticeable seed losses due to pod shattering were not observed for the overripe peas. Standing ability of the peas was considerably improved by mixed cropping. Lodging began later and was less severe at harvest. Within mixtures with 50 % or more faba bean proportions at sowing (relative to the seeding rate of the pure stands) the peas, n i most cases, remained nearly upright until harvest and instead of the usual 'Plattlager', i.e., prostrate but upright top, frequently a more highly inserted 'Hohllager', i.e., upright but collapsed at higher nodes, was observed. In contrast to these remarkably synergistic effects for standing ability, such effects proved to be only small and nonsignificant for grain yield (−1.5–2.9%). Yield stability of the mixed crops was higher than that of the pure stands of both species. Estimates of the stability parameters, ecovalence, deviation from regression and environmental variance were at least halved. Yield proportions of peas and faba beans in the harvested seed of the mixtures did not correspond with the seed proportions at sowing. They differed considerably between seasons and cultivar combination. Higher yield proportions of faba beans were observed under wet and cold weather conditions and for the cultivar combination Victor/Disco, whereas higher yield proportions of peas were found under dry and warm weather conditions and for the cultivar combination Mythos/Baroness .     

Inheritance of seed coat colour in broad bean (Vicia faba L.)

Summary The inheritance of the seed coat colours violet, spotted, brown, green, red, black and beige was investigated in a 10×10 diallel cross between broad bean (Vicia faba L. major) lines. Spotted seed colour was dominant over any uniform seed coat colouring. Brown was dominant over black, green and normal (beige colour). Black and red seed parents behaved as recessive in all F₁ progenies. A 3 (coloured): 1 (normal) segregation ratio was observed in the F₂ of crosses of violet, brown, black, red and spotted seed coat parents to nornal seed coloured parents. Green x beige gave a segregation ratio of 9:7 in F₂. When two parents with different seed coat colour were involved in a cross, the F₂ showed a typical digenic segregation ratio thus demonstrating two unlinked and sometimes epistatic loci.Segregation of a multiallelic series at two loci explains all segregation ratios observed for seed coat colour in broad bean.