猪PRLR和FSHβ基因多态性检测及其与繁殖性状的关联分析

试验旨在研究催乳素受体（prolactin receptor,PRLR）和卵泡刺激素β（follicle-stimulating hormone β,FSHβ）基因的多态性及其与母猪繁殖性状的关联分析。采用PCR-RFLP方法对大白猪、杜洛克猪和长白猪3个猪种共487头母猪进行了PRLR和FSHβ基因多态性检验,并采用单因子方差分析、LSD法分析不同基因型与母猪总产仔数、产活仔数、断奶窝重和断奶窝仔数等繁殖性状的相关性。结果表明,PRLR基因在大白猪和长白猪中B等位基因均为优势基因,基因频率分别为0.717和0.548,大白猪BB基因型总产仔数显著高于AA基因型（P<0.05）,长白猪BB基因型断奶窝重显著高于AB基因型（P<0.05）;FSHβ基因在大白猪、杜洛克猪和长白猪3个猪种中B等位基因均为优势基因,基因频率分别为0.804、0.760和0.789,长白猪BB基因型总产仔数和产活仔数均显著高于AB基因型（P<0.05）,呈现BB>AA>AB趋势。因此,PRLR和FSHβ基因对荣昌猪场母猪繁殖性能有一定影响,可作为母猪繁殖性能分子选育的候选参考基因。     

猪催乳素受体基因第8内含子多态性及其对产仔性能的影响

采用PCR-SSCP技术检测了大白猪、长白猪、杜洛克猪、马身猪、山西黑猪和山西白猪等6个品种364头个体催乳素受体基因(prolactin,PRLR)第8内含子的多态性,并分析了多态位点对猪头胎产仔性能的影响。结果表明,猪PRLR基因第8内含子存在多态性,共检测到A、B、C 3个等位基因和AA、AB、AC、BB、BC和CC 6种基因型。在杜洛克猪、长白猪、山西黑猪和山西白猪群体中,A等位基因的频率最高,分别为0.8276、0.5439、0.5345和0.7669;而在大白猪和马身猪群体中,B等位基因的频率最高,分别为0.6894和0.7000;仅在大白猪、山西黑猪和山西白猪中检测到C等位基因,且频率较低,在0.0379~0.1385之间。PRLR基因型对母猪头胎产仔数有显著影响(P<0.05),对产活仔数的影响未达显著水平。AC基因型母猪的头胎产仔数和产活仔数显著高于BC基因型个体,而与AA、AB和BB基因型个体差异不显著。含有A等位基因的母猪的产仔性能优于不带有A等位基因的个体,表明A等位基因是有利于母猪产仔性能提高的等位基因,可应用于标记辅助选择来提高母猪的繁殖性能。     

催乳素受体基因对大白、长白猪产仔数的影响

采用PCR—RFLP法对大白、长白猪进行催乳素受体（PRLR）基因多态性检测，结果表明，两个品种的PRLR等位基因B频率略高于等位基因A频率，大白母猪PRLR基因型为AA的各胎平均产仔数比AB型高1．62头（P〈0．05），比BB型高2．41头（P〈0．05）；长白母猪AA型比AB型和BB型相应高1.32和3．33头（P〈0．05）。     

Candidate gene markers for litter size in different German pig lines.

Three diallelic RFLP markers at candidate gene loci for litter size, the estrogen receptor (ESR) gene, the prolactin receptor (PRLR) gene, and the retinol-binding protein 4 (RBP4) gene, were evaluated for their association with the number of piglets born alive in different German pig lines. Genotyping was performed on boars and sows belonging to three different genetic groups from a single farm. Information on 8,336 litter records from 2,159 sows (German Landrace, n = 1,672; Duroc, n = 214; and a synthetic line, n = 273) was used in the analyses with respect to litter size. Growth performance traits were only analyzed for the synthetic line. The ESR locus showed no polymorphism in the tested boars of the German Landrace and Duroc lines. In the synthetic line, the frequency for the A allele was 0.90 and no homozygous BB animal was detected. No significant associations of ESR alleles with number of piglets born alive, backfat thickness, or average daily gain were observed. A new PCR-RFLP was developed for testing the PRLR polymorphism. The frequencies of PRLR allele A were 0.40 in the German Landrace, 0.49 in the synthetic, and 0.82 in the Duroc line. In the Duroc line, a small additive effect of the allele B on litter size was observed. The allelic substitution effect was 0.71 piglets born alive across all parities (P = 0.05). No significant associations of the PRLR locus with litter and growth performance traits were detected. The frequencies of RBP4 allele A ranged from 0.62 in the synthetic line to 0.67 in the German Landrace to 0.85 in the Duroc line. For the genotyped sows of the synthetic line, there was no indication of a favorable effect of the A allele with respect to litter size. Results of this study demonstrate that allele effects differ between lines or populations. This may be due to possible different linkage phases between the marker alleles and the causal mutations in the different lines. The results may also be explained by many minor genes affecting litter size. A selection strategy should be designed for each line separately and should always consider possible pleiotropic effects.     

大白猪PTGS2基因外显子2多态性及其与繁殖性状的关联分析

试验旨在研究前列腺素内过氧化物合酶2（prostaglandin-endoperoxide synthase 2,PTGS2）基因的单核苷酸多态性,并将其与大白猪的繁殖性状进行关联分析,为猪分子遗传标记提供依据。以美系及法系纯种大白猪基因组DNA为模板,构建DNA混合池,通过克隆测序比对,检测猪PTGS2基因的遗传变异;采用PCR-RFLP技术对多态性位点进行基因分型,并与目标性状进行关联分析。结果显示,在猪PTGS2基因外显子2上存在1个g.86A>G碱基突变,并具有Msp Ⅰ酶切位点多态性,且在大白猪群体中检测到AA、AG和GG 3种基因型;哈迪-温伯格平衡检验结果显示,在法系大白猪群体中,PTGS2基因g.86A>G多态性分布达到遗传平衡状态（P>0.05）。关联分析结果表明,在美系大白猪群体中,AA基因型初产母猪弱仔数显著少于AG基因型（P<0.05）,AA基因型经产母猪初生窝重显著高于AG基因型（P<0.05）;在法系大白猪群体中,GG基因型个体总产仔数、健仔数和初生窝重均高于AA和AG基因型,但未达到显著水平（P>0.05）。PTGS2基因外显子2 g.86A>G多态性位点显著影响初生窝重和弱仔数,可作为猪分子标记辅助选择育种的潜在位点。     

猪催乳素受体基因第8内含子多态性检测及效应分析

采用PCR-SSCP技术分析了杜洛克猪、长白猪、大白猪、马身猪、山西瘦肉型猪SD-〖KG-*4〗Ⅰ系、SD-〖KG-*4〗Ⅱ系、SD-〖KG-*4〗Ⅲ系和山西白猪等8个猪种372头个体催乳素受体基因（PRLR）第8内含子多态性,检测到A、B、C 3个等位基因和AA、AB、AC和CC 4种基因型。等位基因A在所有品种中频率都较高（0.50~0.62）,等位基因B在杜洛克猪中频率较低（0.12）,在其它品种中频率为中等（0.24~0.44）,等位基因C在杜洛克猪和SD-Ⅲ系中频率较高（0.26）,在其他品种中频率很低（0~0.04）。对AA和CC 2种纯合子进行克隆测序和同源序列比较,发现在扩增片段内有4处突变,即G→A、T→C、A→G的转换突变和TTT插入突变,都发生在PRLR基因的第8内含子。利用140头母猪头胎产仔记录和118头山西白猪后备猪生长发育记录,采用最小二乘分析方法研究了PRLR基因型对猪繁殖性状和生长发育性状的影响,结果表明不同基因型母猪的头胎总产仔数和产活仔数差异不显著。但PRLR基因型对后备猪乳头数、6月龄体重和背膘厚等性状有显著影响,AA型个体的乳头数显著高于AC型（P<0.05）;AC型个体的6月龄体重和背膘厚显著高于AB型（P<0.05）。     

嵊县花猪PRLR基因多态性与繁殖性状的相关分析

应用PCR-RFLP技术检测嵊县花猪PRLR基因第10外显子NaeⅠ酶切位点多态性，并采用SPSS13．0统计软件分析该位点多态性对母猪繁殖性能的影响。结果表明，该位点多态性对头胎、第3和第10胎母猪繁殖性能有显著影响，表现为第1胎AA型母猪产活母仔数和断奶母仔数比AB型母猪多1．68头和1．60头；第10胎AA型母猪产活仔数、活母仔数和断奶母仔数比AB型母猪分别多0．91头，1．67头和1．19头；而第3胎AB型母猪产活仔数、活母仔数和断奶母仔数比AA型母猪分别多1．09头、1．19头和1．05头。     

Effects of prolactin receptor genotype on the litter size of Mangalica

The aim of this study was to detect different alleles of the prolactin receptor (PRLR) gene and to examine their effects on the litter size of the indigenous Hungarian pig, the Mangalica. G1789A single nucleotide polymorphism (SNP) was investigated as a candidate for litter size. Samples from 80 purebred Mangalica sows and data of their 335 litters were provided by Olmos & Tóth Ltd. Hair follicles were used to isolate the required DNA. Allelic discrimination was performed by means of the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method using the AluI restriction enzyme and agarose gel electrophoresis. In the population examined, the A allele was found to be preferable in the Mangalica breed group. The most advantageous AA genotype was the least prevalent (8.75%), while the frequencies of AB and BB were 40% and 51.25%, respectively. Remarkably, the average number of piglets born alive per litter was 1.11 ± 0.39 higher in sows with AA as compared to those with BB genotype. By raising the frequency of the AA genotype, the litter size is likely to increase. However, the effect of PRLR genotypes can differ among pig breeds and even lines. Further studies may be required to observe and estimate possible pleiotropic effects of this polymorphism on other traits.     

4猪种Nramp1基因第6内含子多态性研究

天然抗性巨噬蛋白(Nramp)基因是与人、鼠的一些病原微生物的易感性和抗性有关的重要候选基因。为了研究猪Nramp1基因的多态性,利用PCR-RFLP技术检测了杜洛克、大白猪、长白猪和合作猪共270头个体Nramp1基因第6内含子NdeⅠ酶切位点多态性。结果表明,4个猪种群共检测到3种基因型(AA、AB和BB),其中AB基因型为杜洛克、大白猪和长白猪的优势基因型;AA基因型为合作猪的优势基因型。经卡方适合性检测,杜洛克、合作猪和长白猪处于Hardy-Weinberg平衡状态(P>0.05),大白猪处于Hardy-Weinberg不平衡状态。多态信息含量分析显示,Nramp1基因的第6内含子NdeⅠ酶切位点在各猪种表现出中度多态性。     

猪GPX5,FUT1和PRLR基因的遗传多态性及遗传效应分析

为了分析谷胱甘肽过氧化物酶5基因(Glutathion Peroxidase 5,GPX5),岩藻糖转移酶1基因(α1-Fucosytransferase,FUT1)和催乳素受体基因(Prolactin receptor,PRLR)在野猪与大白猪杂交的F1代群体中的遗传多态性及遗传效应。采用PCR-RFLP方法检测了GPX5,FUT1和PRLR基因的多态性,分析其对初生重(LiB)和30日龄体重(Li30)的遗传效应。结果表明,除了PRLR基因外,其余2个基因的基因频率与大白猪已有研究结果基本一致;PRLR基因的AA型个体在Li30上比AB和BB型个体可显著增重1.42～1.47 kg(P0.05);GPX5和FUT1基因对这2个性状的效应均不显著。结果提示,野猪血统的引入没有显著改变现有猪群的遗传基础,PRLR基因可在生产中考虑应用。     

妊娠期背膘厚对母猪产程及繁殖性能的影响

为优化母猪繁殖效率,探究妊娠期不同背膘厚对母猪繁殖性能和分娩产程的影响,本研究以广西某公司2 969头大白母猪和1 787头长白母猪为试验群体,收集2017年1月至2017年10月妊娠期3个阶段(妊娠30、80和107 d)背膘厚与分娩接产记录数据,分析背膘厚与分娩产程、总产仔数、产活仔数和初生窝重等性状的关系。研究结果表明,大白母猪分娩产程时间显著短于长白母猪(P<0.05),大白母猪总产仔数、产活仔数和初生窝重显著优于长白母猪(P<0.05)。在妊娠30 d时,背膘厚在18～20 mm组的大白母猪总产仔数和产活仔数最高,且初生窝重最大;背膘厚在18～20 mm组的长白母猪总产仔数和产活仔数最高。在妊娠80 d时,背膘厚≥20 mm组的大白母猪总产仔数和产活仔数最高,且初生窝重最大、产程最短;背膘厚在16～18 mm组的长白母猪总产仔数和产活仔数最高,且初生窝重较高、母猪产程较短。在妊娠107 d时,背膘厚在14～16 mm组的大白母猪总产仔数和产活仔数最高,且初生窝重最大,但母猪产程与其他组差异不显著(P>0.05);背膘厚≥20 mm组的长白母猪总产仔数和产活仔数最高,且初生窝重较大。妊娠期背膘厚减少1～2 mm的大白母猪,其总产仔数和产活仔数最高,初生窝重较小,产程较长。而背膘厚减少>2 mm的长白母猪,其总产仔数和产活仔数最高,初生窝重较大,产程较短。上述试验结果说明,在母猪妊娠期间,合适的背膘厚可有效提高母猪繁殖性能和母猪分娩期间的福利水平。养殖场可以根据营养配方和猪群品种建立背膘数据库,通过精准饲喂将母猪背膘厚调整至最佳范围,同时合理控制妊娠期背膘变化。     

猪蛋白酪氨酸磷酸酶-1B基因第7内含子多态性与猪脂肪沉积性状的关联性

本研究旨在分析蛋白酪氨酸磷酸酶-1B(PTP-1B)基因部分SNP与猪脂肪沉积性状的关联性。选取国外瘦肉型猪种大约克夏16头、长白猪21头和中国脂肪型地方猪种梅山猪22头以及"大白×梅山"F2代资源家系213头为研究材料,利用PCR-Eco88I-RFLP方法检测PTP-1B基因第7内含子序列存在G/A突变的多态性,并进行方差分析。结果表明,大白和长白猪种为单一的A等位基因,而梅山猪种以杂合子GA基因型为主,对213头"大白×梅山"F2代资源家系进行性状关联分析,该位点与猪板油质量、内脂率、胸腰椎间背膘厚等脂肪沉积性状显著相关(P<0.05),且杂合子GA基因型个体的板油质量和内酯率都最低(GA基因型个体相似文献   

不同猪种ADIPOQ基因外显子2多态性及其与山西白猪体重和体尺性状的关联分析

本研究旨在检测猪脂联素（adiponectin,ADIPOQ）基因外显子2的多态性,并分析其对山西白猪体重和体尺性状的影响。采用PCR-SSCP技术检测了长白猪、大白猪、杜洛克猪、山西白猪、山西黑猪和马身猪6个猪种392个个体ADIPOQ基因外显子2的多态性,并采用GLM程序分析了ADIPOQ基因外显子2多态性与山西白猪体重和体尺性状的关联性。结果显示,在ADIPOQ基因外显子2的89 bp处检测到G→A错义突变,引起缬氨酸（Val）向异亮氨酸（Ile）的转变。ADIPOQ基因外显子2存在3种基因型：AA、AB、BB,2个等位基因：A和B。杜洛克猪中只有BB基因型,长白猪、大白猪、山西白猪和山西黑猪中BB基因型为优势基因型,马身猪中AA基因型频率最高。在引入品种长白猪、大白猪和杜洛克猪中B等位基因为优势等位基因,基因频率分别为0.96、0.96和1.00;在地方品种马身猪中A等位基因频率（0.52）略高于B等位基因（0.48）;在培育品种山西白猪和山西黑猪中B等位基因频率分别为0.76和0.78,介于引入猪种和地方品种之间。基因型频率分布在马身猪、山西白猪和山西黑猪之间无显著差异（P>0.05）,杜洛克猪与长白猪、大白猪间差异均不显著（P>0.05）,而长白猪和大白猪间差异显著（P<0.05）,任意一个引入品种与马身猪、山西白猪和山西黑猪之间的差异均达到极显著水平（P<0.01）。ADIPOQ基因外显子2多态性对断奶重有显著影响,其中BB基因型个体28日龄断奶重显著高于AA和AB基因型（P<0.05）,AA和AB基因型间无显著差异（P>0.05）,但对其他性状无显著影响,说明该位点只在个体发育早期阶段起作用。     

Relationships between longevity,lifetime productivity,carcass traits and conformation in Polish maternal pig breeds

The objective of this study was to obtain heritability estimates for longevity (length of life, length of productive life, number of litters) and lifetime productivity traits (lifetime pig production, lifetime pig efficiency, lifetime litter efficiency) and genetic correlation between them and litter size at first farrowing, growth (ADG), backfat thickness (BF), loin depth, lean meat percentage (LMP), phenotypic selection index (PSI), and exterior in 19423 Polish Landrace (L) and 16049 Polish Large White (LW) sows. Heritabilities for longevity and lifetime productivity traits were 0.10–0.13 for L sows and 0.09–0.11 for LW sows depending on the trait definition. The genetic correlations among these traits were all high and positive, ranging from 0.76 to 0.99. Antagonistic genetic correlations (?0.21 to ?0.26) were found between longevity traits and PSI and LMP in LW sows, while in L sows the respective parameters were lower and not significant for length of productive life. The number of live‐born piglets in the first litter was positively correlated with lifetime pig production and lifetime pig efficiency in both breeds. The genetic correlations of longevity and lifetime pig production with ADG, BF, loin depth and exterior were small, and in most cases, not significant.     

长白和大白猪主要生长性状的遗传参数估计

旨在分析母猪的出生年份、出生季节、初生重、开测日龄等固定效应对长白、大白猪主要生长性状的影响,并对目标生长性状进行遗传参数估计（遗传力、遗传方差、表型相关和遗传相关）,为猪的遗传改良提供基本依据。本试验利用GLM模型分析试验猪群（398头长白猪和1 176头大白猪）的固定效应对猪生长性状的影响,并采用多性状动物模型对目标性状进行遗传参数估计。目标生长性状包括达100 kg体重日龄（age to 100 kg,AGE）、达100 kg背膘厚（backfat to 100 kg,BF）、100 kg平均日增重（average daily gain to 100 kg,ADG）。研究表明,在大白和长白猪中,猪的出生年、出生季、初生重以及开测日龄对生长性状均具有极显著的影响（P<0.001）;长白猪的AGE、ADG和BF的遗传力分别为0.321、0.327和0.324,大白猪对应性状的遗传力分别为0.454、0.469和0.408;长白猪的ADG和AGE之间的遗传相关、表型相关分别为-0.990、-0.995,大白猪的ADG和AGE之间的遗传相关、表型相关分别为-0.993、-0.998,均呈现较强的负相关。长白、大白猪的生长性状（AGE、ADG、BF）均属于中等遗传力性状,其出生年份、出生季节、初生重和开测日龄对猪的生长性状影响较大。在遗传参数估计分析时,提高样本数量并提升表型数据质量,可以增加遗传参数估计的可靠性。本研究中的生长性状遗传参数估计结果较为可靠,可为后续的遗传改良提供参考。     

Genetic Polymorphism of Dppa5 Gene and its Association with Reproductive Performance in Sow

The purpose of this study was to detect the genetic variation of the porcine developmental piuripotency associated 5 (Dppa5) gene and its association with reproductive performance in sow.PCR-RFLP and sequencing methods were used to detect SNPs and analyze its association with reproductive performance in Large White and Landrace sows.The results showed that two missense mutations g.363 T> C and g.844 G> T were detectd in exon of porcine Dppa5.SNP g.363 T> C affected total number born highly significantly in primiparous Large White sow (P< 0.01) and affected litter size and number born alive significantly in Large White and Landrace sows (P< 0.05).SNP g.844 G> T showed a significant impact on total number born in multiparous Landrace and Large White sows (P< 0.05).The results suggested that the SNPs of Dppa5 gene impacted reproductive performance of sows and could be genetic markers.     

Dppa5基因遗传多态性及其与母猪繁殖性状的关联分析

试验旨在研究猪多潜能性维持因子5(developmental piuripotency associated 5,Dppa5)基因的遗传多态性及其与母猪繁殖性状的遗传效应。以长白猪和大白猪为试验群体,采用PCR-RFLP、克隆测序等方法分析检测猪Dppa5基因的多态性并对其与母猪产仔性能进行关联分析。结果表明,猪Dppa5基因外显子上存在2个错义突变位点:g.363 T> C和g.844 G> T;猪Dppa5基因g.363 T> C 位点对初产大白猪的总产仔数有极显著影响(P< 0.01),对经产大白猪、长白猪的产活仔数有显著影响(P< 0.05);g.844 G> T位点对经产长白猪和大白猪的总产仔数有显著影响(P< 0.05)。综合结果分析,Dppa5多态位点对猪的繁殖性能存在影响,可作为潜在的遗传标记,为猪的分子育种提供参考。     

大白猪BMPR-ⅠB基因多态性及其与繁殖性状的相关性研究

试验旨在研究不同品系大白猪骨形态发生蛋白受体ⅠB（bone morphogenetic protein receptor-ⅠB,BMPR-ⅠB）基因多态性及其与繁殖性状的相关性。采用Hi-SNP中高通量基因分型技术对BMPR-ⅠB基因多态位点进行分型,利用SPSS统计软件,采用最小二乘法将各位点不同基因型与总产仔数、产活仔数、死胎数、畸形头数进行相关性分析。结果显示,不同品系大白猪在BMPR-ⅠB基因746A > G和852G > A位点均未检测到多态性,804G > C位点上均表现为GG基因型为优势基因型,G为优势等位基因;960C > T位点在美系大白猪中以CC基因型频率较高,而在英系大白猪中以TT基因型频率较高,在加系大白猪中CT基因型频率较高,3个不同品系大白猪群体在804G > C和960C > T位点均处于Hardy-Weinberg平衡状态（P > 0.05）。804G > C位点在3个不同品系大白猪中对总产仔数、产活仔数、死胎数、畸形数等繁殖性状均无显著影响（P > 0.05）。960C > T位点在美系大白猪群体中TT基因型死胎数极显著低于CC基因型1.027头（P < 0.01）;英系大白猪群体中TT基因型总产仔数显著高于CC基因型1.976头（P < 0.05）,极显著高于CT基因型2.118头（P < 0.01）;加系大白猪中TT基因型产活仔数显著高于CC基因型0.886头（P < 0.05）,死胎数、畸形头数显著低于CC基因型0.707和0.337头（P < 0.05）。960C > T位点中TT基因型可作为3个不同品系大白猪繁殖性状的标记基因型。     

Genetic associations of prolificacy with performance, carcass, meat quality, and leg conformation traits in the Finnish Landrace and Large White pig populations

The objective of this study was to estimate genetic associations of prolificacy traits with other traits under selection in the Finnish Landrace and Large White populations. The prolificacy traits evaluated were total number of piglets born, number of stillborn piglets, piglet mortality during suckling, age at first farrowing, and first farrowing interval. Genetic correlations were estimated with two performance traits (ADG and feed:gain ratio), with two carcass traits (lean percent and fat percent), with four meat quality traits (pH and L* values in longissimus dorsi and semimembranosus muscles), and with two leg conformation traits (overall leg action and buck-kneed forelegs). The data contained prolificacy information on 12,525 and 10,511 sows in the Finnish litter recording scheme and station testing records on 10,372 and 9,838 pigs in Landrace and Large White breeds, respectively. The genetic correlations were estimated by the restricted maximum likelihood method. The most substantial correlations were found between age at first farrowing and lean percent (0.19 in Landrace and 0.27 in Large White), and fat percent (-0.26 in Landrace and -0.18 in Large White), and between number of stillborn piglets and ADG (-0.38 in Landrace and -0.25 in Large White) and feed:gain (0.27 in Landrace and 0.12 in Large White). The correlations are indicative of the benefits of superior growth for piglets already at birth. Similarly, the correlations indicate that age at first farrowing is increasing owing to selection for carcass lean content. There was also clear favorable correlation between performance traits and piglet mortality from birth to weaning in Large White (r(g) was -0.43 between piglet mortality and ADG, and 0.42 between piglet mortality and feed:gain), but not in Landrace (corresponding correlations were 0.26 and -0.22). There was a general tendency that prolificacy traits were favorably correlated with performance traits, and unfavorably with carcass lean and fat percents, whereas there were no clear associations between prolificacy and meat quality or leg conformation. In conclusion, accuracy of estimated breeding values may be improved by accounting for genetic associations between prolificacy, carcass, and performance traits in a multitrait analysis.     

江口萝卜猪和梅山猪的猪应急综合征主效基因RYR1的PCR-RFLP检测

猪兰尼定受体1(Ryanodine Receptor1,RYR1)基因是导致猪应激综合征、影响猪肉质的主效基因.试验采用RFLP方法,以大白猪、2个杂交猪群(杜洛克×长白猪×大白猪和长白猪×大白猪)为对照,检测了江口萝卜猪与引入的梅山猪群RYR1基因的分布类型.结果表明,对照组中,外三元、外二元杂交猪和大白猪中均检测到...