湖北省襄樊地区规模化猪场猪瘟抗体水平监测

应用ELISA方法对襄樊地区2007年4～8月份31家猪场323份血清进行猪瘟抗体水平监测。试验结果表明:猪瘟抗体水平合格猪场9家,合格率29.0%,猪瘟抗体免疫合格血清140份,合格率43.3%。     

用Dot—ELISA对猪瘟抗体水平监测的报告

采用猪瘟Dot-ELISA诊断试剂盒对湖北省3个规模化猪场猪瘟抗体水平进行监测,以了解通过目前我国常用猪瘟免疫程序注射疫苗后猪群的免疫状况;同时分析了目前我国猪瘟流行特点,即由急性爆发型向温和型猪瘟发展趋势,指出只有通过正确免疫程序,加强疫苗管理,采用综合防治,才能控制和消灭猪瘟;通过实验操作和结果表明Dot-ELISA猪瘟诊断试剂盒具有微量、简便、快速、敏感等特性,是适用于基层现场检测猪瘟较好方法之一.     

规模化猪场母猪群猪瘟抗体水平监测

利用正向间接血凝试验,检测了广西来宾市的5个规模化猪场的864头经产母猪的猪瘟免疫抗体水平。结果表明:在规模化猪场的864份血清中,免疫合格有797份,免疫合格率达92%;其中A场的260份血清中,有248份免疫合格,合格率为95.3%;B场的180份血清中,有162份免疫合格,合格率为90%;C场174份血清中,有157份免疫合格,合格率为90.2%;D场155份血清中,有148份免疫合格,合格率为95.5%;E场95份血清中,有82份免疫合格,合格率为86.3%。80%的猪场都达到90%的免疫合格。     

山东省诸城市规模化猪场猪瘟抗体水平的监测

猪瘟是由猪瘟病毒引起的猪的一种急性、热性、接触性传染病。本文对山东省诸城市13个规模化猪场共780份血清进行了抗体监测,结果显示2012年4月、8月、12月的猪瘟抗体水平平均合格率分别为90%、91%、95%,各规模化猪场免疫抗体水平全部达到国家免疫抗体保护水平。监测结果为山东省诸城市有效控制猪瘟提供了科学依据。     

应用间接ELISA检测并分析某规模猪场猪瘟抗体水平

应用猪瘟病毒抗体间接ELISA检测试剂盒,对湖南省洞口县某规模猪场送检的100份血清进行猪瘟病毒抗体检测。结果显示,猪场的检测阳性率为77%。据此可对该猪场猪只免疫状况进行动态监测。     

某猪场猪瘟抗体水平检测及反思

近年来各养殖场重视疫病防控工作,尤其是疫苗免疫接种。某猪场对饲养的猪针对8种疾病用6种疫苗进行免疫接种。结果是急性暴发性典型性疫病越来越少,而慢性非典型性疫病在不断增多,以猪瘟为例,猪场管理者认为猪瘟免疫从未有半点懈怠,却每年都有慢性猪瘟发生,为了了解和掌握该猪场的猪瘟免疫后抗体产生情况,笔者对该场不同年龄的猪采血,用正向间接血凝方法检测猪瘟抗体,结果表明1岁以上的母猪抗体水平较高,3月龄以下的猪抗体水平非常低,这是免疫时机掌握不当,还是另有原因正是我们关注的。1材料与方法1.1被检材料:耳静脉采猪血,分离血清,低温…     

部分猪场猪瘟抗体有效保护水平的探讨

猪瘟抗体水平达到1∶16是免疫是否合格的判断标准,而作者通过对疑似猪瘟的猪分别进行猪瘟抗原和抗体测定,发现感染野毒的猪部分抗体水平在1∶16以上,有些甚至在1∶64以上,仍然感染发病,所以建议种猪猪瘟抗体免疫合格标准应提高到1∶64,其它猪应提高到1∶32。     

新疆石河子地区规模化猪场猪瘟抗体检测及分析

为了解新疆石河子地区猪场猪瘟病毒(CSFV)抗体水平,试验从新疆石河子地区的12个不同规模猪场共采血样743份,采用间接ELISA(酶联免疫吸附试验)法检测CSFV的抗体水平。结果发现,该地区CSFV抗体平均阳性率为85.87%(638/743),免疫抗体阳性率超过80.00%的猪场占75%,各猪场抗体水平差异极显著(p0.01)。结果表明石河子地区CSFV抗体水平较高,该结果可为新疆石河子地区猪瘟防疫工作提供一定的参考。     

闽西地区规模化猪场猪瘟抗体水平监测及免疫效果分析

应用猪瘟正向间接血凝试验 ,检测了闽西地区规模化猪场 10 93份血清的猪瘟抗体水平。结果表明 ,猪瘟血清抗体水平≤ 1∶ 8的有 2 6 7份 ,免疫合格率为 75 .5 %。哺乳仔猪检测 10 7份 ,猪瘟血清抗体水平≤ 1∶ 8的有 34份 ,免疫合格率为 6 8%。断奶仔猪检测 333份 ,猪瘟血清抗体水平≤ 1∶ 8的有 12 3份 ,免疫合格率为 6 2 %。育肥猪检测 34 0份 ,猪瘟血清抗体水平≤ 1∶ 8的有 94份 ,免疫合格率为 72 %。母猪检测 313份 ,猪瘟血清抗体水平≤ 1∶ 8的有 16份 ,免疫合格率为 95 %。所有猪群中断奶仔猪免疫合格率最低 ,与临床上断奶仔猪猪瘟发病率最高相吻合。     

规模化猪场猪瘟抗体水平监测和免疫效果分析

应用ELISA检测来自柳州市3个大型规模猪场共435份血清中的猪瘟抗体,其中57份血清猪瘟抗体OD值低于30,占13．1％;有44份血清OD值在30-40之间,占10．1％;有334份血清猪瘟OD值大于或等于40,占76．8％。育肥猪和种猪群的抗体阳性率分别为67．5％和88．2％。种猪的免疫效果比育肥猪好。     

The hog cholera virus.

Hog cholera virus (HCV) is a spherical enveloped particle of about 40-60 nm dia. The viral genome is a single strand RNA of about 12,000 bases with positive polarity. One single large open reading frame codes for presumably four structural, i.e. three glycoproteins and a core protein, and about three to five nonstructural proteins. The functional role is not yet fully clear for all viral proteins. HCV belongs to the pestivirus group and it is closely related to bovine viral diarrhoea and border disease viruses. The relationship extends to morphology, antigenicity, host spectrum and molecular properties. Pestiviruses hold generic status in the family Flaviviridae.     

Transmission of hog hog cholera virus by mosquitoes.

Mosquitoes trapped during an epizootic of hog cholera (HC) in Maryland in 1969 were prepared into 40 pools which were inoculated in pigs. Hog cholera virus was confirmed in pigs inoculated with 8 of 40 pools of mosquitoes. Generally, the pigs contracting HC developed chronic infections with persistent viremia that lasted 30 or more days. Two pigs seemed healthy when euthatized 62 and 80 days after inoculation, yet viremia of high titer was detected in each. Experimental studies were performed with 2 laboratory strains of mosquitoes, Aedes aegypti and Culex tarsalis, to determine if biological and mechanical transmission occur. Biological transmission was not confirmed, but HC virus was retained in A aegypti for 3 days. Mechanical transmission was confirmed with A aegypti in 2 of 9 experiments.     

Host immune responses against hog cholera virus in pigs treated with an ionized alkali mineral complex

To determine the immune responses in pigs to hog cholera virus after treatment with an ionized alkali mineral complex (IAMC), 40 healthy pigs (28-32 days old) from a commercial swine farm were purchased and housed into 4 groups (n=10 each). All pigs were vaccinated intramuscularly (1 ml) with an attenuated live hog cholera virus (HCV, LOM strain) at 28-32 days old and challenged with a virulent hog cholera virus at 8 weeks after vaccination. Each group was treated with PowerFeel sprayed diet as 0.05% (w/w) in a final concentration (T-1, n=10), a diet mixed with SuperFeed as 3% (w/w) in a final concentration (T-2, n=10), or a diluted PowerFeel solution (1:500, v/v) as drinking water (T-3, n=10), respectively. A group (n=10) served as a non-treated control. Proportions of expressing CD2+ and CD8+ cells increased significantly (p<, 0.05) at 8-week post-application. Mean antibody titers of each group against HCV gradually increased to higher levels after vaccination and with challenge of the virulent virus. In conclusion, the IAMC-treated diets can be helpful for the improvement of growth in pigs with proper vaccination program, while the IAMC-treated diets have no effects on the clinical protection against hog cholera.     

猪瘟病毒与猪附红细胞体混合感染的诊治

2005年7月,梧州市长州区倒水镇某村猪群暴发以高热、皮肤发红,腹下及四肢下部出现出血斑点为主要症状的疾病.根据临床症状、剖检变化及实验室检验等综合诊断,确诊为猪瘟与猪附红细胞体病混合感染,现报告如下:     

Differentiation of hog cholera and bovine virus diarrhoea viruses in pigs using monoclonal antibodies

Monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. The animals had been infected simultaneously with both viruses. The antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. A polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. Both techniques based on monoclonal antibodies were more sensitive and more specific than the conventional test, the enzyme immunoassay being more sensitive than the immunofluorescence test. Small amounts of BVD viral antigen were demonstrable with monoclonal antibodies in most organ tissues.     

Stabilization of hog cholera virus by dimethyl sulfoxide.

The stability of hog cholera virus through five freeze-thaw cycles in the presence and absence of dimethyl sulfoxide was studied. In the absence of dimethyl sulfoxide the hog cholera virus titer was reduced 52% to 91% following successive freezing and thawing cycles. However, when dimethyl sulfoxide was added to the viral suspension the virus titer appeared to remain the same after the same number of freezing and thawing cycles.