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Evaluation of genetic relationship among six Labeo species using randomly amplified polymorphic DNA (RAPD) 总被引:1,自引:0,他引:1
Paramananda Das Harendra Prasad Prem Kumar Meher Ashoktaru Barat & Ranjit Kumar Jana 《Aquaculture Research》2005,36(6):564-569
Labeo species constitute an important group of fish with intense diversity and potential for commercial aquaculture in many Southeast Asian nations including the Indian subcontinent. The present investigation involves the comparative analysis of randomly amplified polymorphic DNA (RAPD) profiles of six Labeo species viz., L. bata (bata), L. calbasu (calbasu), L. dyocheilus (dyocheilus), L. fimbriatus (fimbriatus), L. gonius (gonius) and L. rohita (rohu) at the nuclear DNA variation level. Fifteen decamer random primers were chosen from 40, which amplified a total of 449 DNA fragments ranging in size from 400 to 3000 bp. Both monomorphic and polymorphic DNA bands were identified based on their presence or absence that could be used for specieswise differentiation. Similarity coefficients were calculated to quantify the genetic variation within and between species. On an average, the highest intra‐species genetic similarity value was found in calbasu (0.93) followed by rohu and fimbriatus (0.91), bata (0.87), gonius (0.86) and dyocheilus (0.77). The interspecies genetic similarity estimates among the species of Labeo were used to deduce their phylogenetic relationships. The cluster analysis showed two main clusters, one with calbasu, rohu, fimbriatus and gonius and another with bata and dyocheilus. The study provides evidence that RAPD could be used for genetic differentiation of closely related species. 相似文献
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Teleost fish lack the enzyme for endogenous synthesis of ascorbic acid (AA), an essential micronutrient for fish. The aim
of this study was to examine the effect of higher levels of dietary vitamin C on growth, nutritional quality, and immunomodulation
in the Indian major carp, rohu (Labeo rohita). Four groups of L. rohita were fed experimental diets containing either no vitamin C (control) or supplemented with vitamin C at 500 mg kg−1 (Exp-1), 1000 mg kg−1 (Exp-2), or 1500 mg kg−1 (Exp-3) for 60 days. Growth parameters (NWG, ADG, and SGR), serological parameters (TSP, TSA, TSG, and A:G), haematological
parameters (TLC, TEC, Hct, MCV, and MCH), and different non-specific immunological parameters (PR, PI, respiratory burst activity,
and bactericidal activity) were evaluated during the experimental trial. Fish fed a vitamin C-supplemented diet showed higher
specific growth rate (SGR) up to 1000 mg kg−1 compared with control fish. Different haematological and serological parameters along with non-specific immune parameters
were influenced by vitamin C supplementation. Among the non-specific immune parameters phagocytic activity (PR and PI) and
respiratory burst activity (NBT cells) were significantly (P ≤ 0.05) enhanced by increasing doses of vitamin C supplementation. Higher levels of dietary vitamin C significantly (P ≤ 0.05) enhanced protection against Aeromonas hydrophila (AH1) infection compared with controls. Results from this study help to establish the beneficial effect of vitamin C on growth
and immunmodulation in rohu (L. rohita). 相似文献
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T. T. Chen L. B. Agellon C. M. Lin H. J. Tsai Peijun Zhang L. I. González-Villasénor D. A. Powers 《Fish physiology and biochemistry》1989,7(1-6):381-385
The primary structures of two rainbow trout growth hormone mRNAs (GH1 and GH2) have been deduced by direct sequencing of their
respective cDNA clones and portions of the mRNA. Both GH1 and GH2 mRNA contain open reading frames comprised of 630 nucleotides
and encode 210 amino acid residues of which 11 are variant. The translated regions of both mRNA are flanked by a short but
rather conserved 5′-end, and a relatively long but highly diverged 3′-end. The differences at translated and 3′-untranslated
regions suggest that the GH1 and GH2 mRNA originate from different loci. The GH1 and GH2 mRNA are likely transcribed from
two distinct loci which were duplicated during tetraploidization of salmonid genome between 50 to 100 million years ago.
The GH2 gene has been isolated and sequenced from a rainbow trout genomic library. This gene spans a region of approximately
4 kilobases. The trout GH gene is comprised of 6 exons and 5 introns, in contrast to 5 exons and 4 introns in mammals. The
additional intron in the trout gene interrupts the translated regions that are analogous to the last exon of the mammalian
counterpart. The alleged internally repeating sequences in mammalian GH, prolactin (Pr1) and placental lactogen (PL) are not
observed in the predicted polypeptide sequence of trout GH. In addition, direct repeats that flank exons I, III and V of mammalian
GH, Pr1 and PL genes are absent in trout gene. These findings indicate that the rainbow trout GH gene structure does not support
the current hypothesis that internally repeated regions in GH, Pr1 and PL arose from a small primordial gene. 相似文献
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Marieke Verleih Alexander Rebl Bernd Köllner Tomáš Korytář Günter Kotterba Eckhard Anders Klaus Wimmers Tom Goldammer 《Fish physiology and biochemistry》2010,36(4):1271-1276
The proline-rich protein 13 (PRR13) is reported to be a key regulator of the resistance to cytostatica by decreasing the copy
number of the proapoptotic gene thrombospondin-1. We isolated and characterized the complete PRR13 gene sequence of rainbow
trout (Oncorhynchus mykiss). The gene comprises four exons and three introns, the latter of comparatively short lengths (100–811 bp). The full-length
PRR13 cDNA consists of 1,101 nucleotides, including an open reading frame of 563 bp, which is predicted to encode a 187 amino
acid protein with a molecular mass of 18.8 kDa. A continuous stretch of ten serine residues at the C-terminus is highly conserved
and characteristic for vertebrate PRR13, but not for other known proline-rich proteins. Phylogenetic analyses suggest a clear
separation of teleostean PRR13 proteins and those from mammalian and reptilian species. Comparison of the tissue-specific
PRR13 mRNA abundance in two strains of the rainbow trout coastal form (TCO Steelhead II-WA vs. BORN Steelhead II-Germany)
revealed an increased expression in the BORN trout in nearly all examined tissues. The major expression differences were detected
in gill (2.29-fold) and in liver tissue (2.16-fold). Hence, the increased PRR13 expression in BORN trout might cause improved
protection from natural cytostatica and therefore support our assumption that PRR13 is a candidate gene possibly involved
in the varying ability of the two rainbow trout strains to handle environmental stress under local conditions of the Southern
Baltic. 相似文献
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The number of growth rings was counted on broken and burnt otoliths of eight Lethrinus spp. from two local populations: Lethrinus atkinsoni, L. harak, L. miniatus, L. nebulosus, L. obsoletus, L. ornatus, L. ravus, and L. rubrioperculatus, collected from 1985 to 1996 in waters off the Ryukyu Islands. Growth rings were revealed to be formed annually from about
October to June by marginal analysis. In addition to obtaining the three parameters in von Bertalanffy growth equation and
the maximum age in specimens, the relationship between age and both ovarian maturity rate and sex ratio (percentage female)
was analyzed. The age at 50% of ovarian maturity was the lowest at 1–2 years old in L. harak, L. ravus, and L. rubrioperculatus and was the highest at approximately 4 years old in L. nebulosus. The age at which the sex ratio decreased to 50% due to sexual transition from female in protogynous hermaphrodite species
was the lowest at 3–4 years old in the L. atkinsoni Okinawa population and was the highest at 7–8 years old in L. miniatus. The oldest maximum age for specimens was 26 years in L. nebulosus and the youngest maximum age was 12 years in L. ornatus. 相似文献
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The present study was undertaken with a view to compare the precision and reliability of the age readings obtained from different bony structures of some important freshwater teleosts viz., Labeo rohita (Hamilton), Catla catla (Hamilton) and Channa marulius (Hamilton). Standard procedures were followed to prepare and study the age structures. In L. rohita and C. marulius percent agreement between reader's age estimates was highest for scales, i.e. 96.3% and 90.5%, respectively and in C. catla percent agreement was highest (93.3%) for opercular bone. When scale ages were compared with other alternative structures viz., otoliths, opercular bone, vertebral centra and dorsal fin rays, percent agreement was found highest between scale and opercular bone age estimates (77.8%) in L. rohita and between scale and otoliths (94.8%) in C. marulius. In case of C. catla highest percent agreement was found between opercular bone and scale age estimates. In L. rohita each of the ageing structure showed significant (P < 0.05) underestimation of age in comparison to scales. In C. catla mean age estimates from opercular bone were comparable (P > 0.05) to the values obtained from all other structures except dorsal fin rays. In C. marulius mean age estimates from scales were comparable (P > 0.05) to those from all other structures except from dorsal fin rays. Results indicated scales to be the most suitable structure for ageing L. rohita and C. marulius and opercular bone for C. catla. However, in C. catla also scales may be used as a non-destructive method of age estimation with satisfactory results. 相似文献
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Aquatic weeds are one of the major unconventional feed ingredients tested for aquafeed formulation. Tannin content in the
water lettuce, Pistia, has been quantified (26.67 mg g−1; dry weight) and graded levels of which (12.5–200 μg) have been incorporated in the reaction mixtures to evaluate any change
in the in vitro activity of the principal digestive enzymes from the three Indian major carps (IMC), namely rohu (Labeo rohita), catla (Catla catla) and mrigala (Cirrhinus mrigala). Result of the experiment revealed that the Pistia tannin (PT) significantly inhibit/lower the activities of the digestive enzymes from three IMCs in a dose-dependant manner,
even at very low concentration. Significant variation in the reduction of the enzyme activities was noticed between the three
fish species, as well as between the three enzymes studied. Among the three species studied, digestive enzymes from L. rohita were found to be the most sensitive to the PT, whereas enzymes from C. catla were found to be comparatively least affected. On the other hand, protease and lipase activities were comparatively more
affected than the amylase activity. The results of the study suggest that more stress should be given on the elimination of
tannin while incorporating feed ingredients of plant origin in fish diets. 相似文献
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翘嘴鲌两种生长激素受体基因结构及微卫星多态性与生长性状的相关性 总被引:1,自引:0,他引:1
为了更好地研究翘嘴鲌两种生长激素受体(GHR)的结构和功能,实验以翘嘴鲌转录组中获得的mRNA为基础,对其DNA序列进行了克隆。在进行生物信息学分析的同时,对其中的多态性微卫星位点在120尾同批繁殖、同塘养殖的翘嘴鲌个体中进行了分析。GHR1的cDNA序列长度为3 498 bp,开放阅读框(ORF)为1 818 bp,编码605个氨基酸;GHR2的cDNA序列长度为1 743 bp,ORF为1 743 bp,编码580个氨基酸;GHR1和GHR2氨基酸序列均由信号肽、胞外区、跨膜区、胞内区组成,相似度为37.2%。二者在结构上存在较大的差异:GHR1胞外区有7个半胱氨酸残基,而GHR2只有5个,且GHR1比GHR2多3个N-糖基化位点;在胞内区,GHR1存在10个酪氨酸残基而GHR2只有5个,这些差异表明二者可能具有不同的生物学功能。同源氨基酸序列比对发现,GHR与其他鲤科鱼类的同源基因保守性较高。翘嘴鲌2个GHR各包含9个内含子,其中GHR1内含子1和2序列在10 kb以上,本实验没有对其进行扩增。所获得的序列中共发现了6个微卫星位点:GHR1中微卫星位点(CT)6 相似文献
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The complement component 9 (C9) plays a significant role in the formation of membrane attack complex (MAC) on the targeted cell surface. The current study is dealt with molecular characterization of C9 gene from rohu, Labeo rohita, an important cultured carp species in India. An open reading frame (ORF) of 1998 bp was amplified by polymerase chain reaction (PCR) that encodes a polypeptide of 666 amino acids having a signal peptide of 19 amino acids and a mature peptide of 647 amino acids. The SMART domain architecture analysis revealed two thrombospondin type‐1 domains (TSP1), a low‐density lipoprotein receptor domain class A (LDLa), a membrane attack complex and perforin (MACPF) domain, and an epidermal growth factor (EGF)‐like domain. Multiple sequence alignment and evolutionary analysis revealed a primitive C9 sequence of rohu with maximum similarity and clustering with common carp, grass carp and zebrafish. C9 was highly expressed in liver and constitutively expressed in wide array of tissues except in eye of rohu juveniles. It was expressed during early developmental days of rohu including in milt. A variable level of up‐regulation in C9 expression was noticed upon poly I:C induction, Aeromonas hydrophila and Argulus siamensis infections in liver, spleen and gill tissues of rohu at different time points. A constitutive expression of C9 in different stages of rohu during the ontogeny and in response to pathogen exposures along with high degree of sequence homology with other fish species proved it as an important primitive immune molecule of the complement system lytic pathway. 相似文献
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Genomic structure and molecular characterization of growth hormone and its expression response to different feed types in golden pompano Trachinotus ovatus (Linnaeus, 1758)
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Yin‐Yin Liang Hua‐Yang Guo Ke‐Cheng Zhu Nan Zhang Jing‐Wen Yang Xiao‐Xiao Sun Shi‐Gui Jiang Dian‐Chang Zhang 《Aquaculture Research》2018,49(5):1973-1986
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Imtiaz Ahmed 《Aquaculture International》2011,19(3):567-584
An 15 week two set of feeding experiments were conducted to determine the dietary niacin requirement of Indian major carp
fingerlings Labeo rohita and Cirrhinus mrigala, using casein gelatin–based diet. In both experiments, six isonitrogenous (40%) and isoenergetic (15.35 kJ g−1) test diet, with graded levels of niacin (0–50 mg kg−1 dry diet) in gradation of 10 mg kg−1 dry diet, were formulated. In first experiment, fingerling of L. rohita (4.20 ± 1.22 cm; 0.632 ± 0.67 gm) were randomly stocked, in triplicate groups, in 55-L indoor polyvinyl flow-through system
(1.5 L min−1) and fed experimental diet at 0800 and 1600 h. Maximum live weight gain (1214%), feed conversion ratio (1.55) and protein
efficiency ratio (1.60) were recorded at 30 mg dietary niacin diet. In second experiment, C. mrigala (4.50 ± 1.25 cm, 0.665 ± 0.88) were stocked in same setup. At the end of experiments, maximum live weight gain (1248%), FCR
(1.47) and PER (1.70) occurred at 30 mg dietary niacin diet. However, the weight gain, FCR and PER data were analyzed by polynomial
regression analysis indicating the requirement of niacin for L. rohita at 36.69, 33.06 and 32.0 mg kg−1, respectively, and for C. mrigala at 35.19, 28.69 and 27.70 mg kg−1 of dry diet, respectively. Whole body composition also showed significant (P < 0.05) differences among each other. On the basis of regression analysis of growth data, it is recommended that the diet
for fingerlings should contain niacin at 33 and 30 mg kg−1 dry diet for L. rohita and C. mrigala, respectively. 相似文献