Regeneration dynamics of planted seedling-origin aspen (<Emphasis Type="Italic">Populus tremuloides</Emphasis> Michx.)

Aspen (Populus tremuloides Michx.) is a foundational tree species of the North American boreal forest. After disturbance, clonal aspen stands quickly achieve canopy closure by sending up numerous clonal shoots (root suckers) from their lateral root system. Controlled aboveground disturbance will commonly induce prolific root suckering and thereby increase stem density in clonal aspen stands, but it is unclear if increases in stem density will be observed in planted seedling-origin aspen stands. The objectives of this study were to determine (1) overall root suckering response of planted aspen to aboveground disturbance; (2) if different cut heights of the stem or infliction of root damage impact the number of root suckers produced. We found that planted aspen regenerated readily after disturbance, averaging five root suckers per cut tree. However, individual response was highly variable, ranging from zero to 29 root suckers per root system. Of the cut trees, 75% produced at least one root sucker and 60% produced at least one stump sprout. Cutting trees close to the soil surface produced more root suckers than leaving a 25 cm stump. While root system size (mass and length) was well correlated with aboveground measures of planted aspen, root suckering was not related to root system size. As a result of increased forest reclamation efforts in the boreal forest region the planting of aspen has become a more common practice, necessitating a better understanding of the regeneration dynamics and root suckering potential of these planted and seedling-origin aspen forests.     

Clonal variation in root suckering ability of hybrid aspen (Populus tremula L. × P. tremuloides Michx.)

Current information on the root sucker ability of hybrid aspen (Populus tremula L. × P. tremuloides Michx.) is in most cases based on clone mixtures. In this study, we could separate the performance among clones by using two experimental sites with monoclonal plots of the crossing. The natural regeneration was followed for 2 years after harvest of the formerly planted stands, clear-cut at 22 and 25 years of age, respectively. We found that clonal differences were present in the number of root suckers produced per area unit and their biomass production. However, all included clones produced sufficiently many root suckers for a successful regeneration. To receive a more complete evaluation of the root sucker dynamics for future selection on the clonal level, further studies are needed where vitality, self-thinning and effects of thinning measures are coupled to the goal of the aspen forestry.     

Regeneration of Populus nine years after variable retention harvest in boreal mixedwood forests

Aspen and balsam poplar regeneration from root suckers were assessed in boreal mixedwood forests nine years after logging in a variable retention experiment (EMEND Project—Ecosystem Management Emulating Natural Disturbance) located north of Peace River, Alberta, Canada. Five levels of retention of mature trees (2%, 10%, 20%, 50% or 75% of the original basal area) were applied in stands dominated by aspen, white spruce or mixtures of the two species. Basal area of aspen (or that of aspen plus balsam poplar combined) prior to logging strongly influenced sucker density of aspen (or aspen + balsam poplar combined) and in some cases their growth. Nine years after harvest there was a decline in sucker density and volume ha⁻¹ with increasing retention levels of aspen (or both poplars combined); sucker density declined by 50% when only 20% of the original basal area was left in the stand. Retaining mature spruce trees in the stand had little influence on the number of suckers but did affect their total volume ha⁻¹. Thus, we suggest that by knowing stand aspen and balsam poplar density prior to logging and varying levels of retention of aspen and balsam poplar or conifers at harvest, the density of Populus regeneration can be predicted by managers, thereby allowing them to create a range of mixedwood conditions.     

Root carbohydrates and aspen regeneration in relation to season of harvest and machine traffic

Season of harvest has often been suggested as a driver for the erratic success of aspen (Populus tremuloides) sucker regeneration, partially due to root carbohydrate reserves and soil conditions at the time of harvest. A field experiment in western Manitoba, Canada, assessed root suckering and root carbohydrates of aspen in response to season of harvest and machine traffic. Six sites (120 m × 120 m) were selected within two large mature aspen stands slated for summer harvest. Plots (50 m × 50 m) were hand-felled (without machine traffic) in mid-summer, late summer, winter, and one plot was left uncut as a control. Season of cut with no traffic had no effect on sucker density, height or leaf dry mass per sucker. During the dormant season, root starch reserves were highest in the winter cut plots, however, just prior to suckering, this difference in carbohydrate reserves among the three seasons of harvest disappeared and by the end of the first growing season root reserves in all three seasons of cut had recovered to near control levels. Adjacent plots that were conventionally harvested in the summer and impacted by logging traffic had similar sucker densities but had 19% less height growth of suckers and 29% less leaf dry mass per sucker compared to suckers in plots harvested at the same time without traffic. After one growing season, root carbohydrate levels were similar whether or not machine traffic was used; however, the reduction in leaf dry mass in plots with machine traffic could have negative implications for carbohydrate accumulation and growth. The study suggests that the phenological state of the mature aspen plays a very small role in aspen regeneration and that harvesting practices and site conditions are likely the main drivers of aspen regeneration success.     

Effects of soil temperature and time of decapitation on sucker initiation of intact Populus tremuloides root systems

Abstract

In a growth chamber experiment, root suckering of aspen (Populus tremuloides Michx.) was assessed in relation to timing of cutting and soil temperature. Aspen seedlings were grown in large pots for 3 years before experimentation. In a 2×2 factorial experiment, 3-year-old seedlings were cut at the end of the dormant period or after leaf flush and grown at two soil temperatures (8 or 20°C) for 39 days. Root systems were evaluated for suckering response and carbohydrate reserve status. There were no differences between the two soil temperatures and times of cut in the number of sucker buds initiated on the roots, but the number of buds that developed into suckers was much greater at 20°C. Cutting the dormant seedlings delayed suckering by nearly a week, resulting in smaller suckers at the time of harvest. However, cutting the seedlings when dormant produced almost twice the number of suckers than when cutting occurred after leaf-out. Total non-structural carbohydrates (TNC) of roots declined from 35.6% of dry weight at the end of the dormant season to 21.6% at the time of leaf-out, but there were no differences between the soil temperature and timing of cut treatments. After the 39 day growth period, root systems had 7% lower root TNC in the 20°C treatment than in the 8°C treatment, likely to support the development of the emerging suckers and higher respiration demands.     

Suckering response of aspen to traffic-induced-root wounding and the barrier-effect of log storage

In a growth chamber, we tested how the seasonal timing of placing a physical barrier (simulating a possible effect of log storage) and inflicting root damage impacted aspen (Populus tremuloides Michx.) root systems and their suckering capability. Roots from 4-year-old saplings were used, and one half of these root systems had the above-ground portion cut in the winter (dormant) while the other half was cut during the growing season in the summer. Damage was inflicted to the roots by driving a large farm tractor over them, and a covering treatment was applied using a polystyrene board to prevent suckers from emerging from the soil. Soil temperatures for the winter-cut root systems were kept at 5 °C over the growing season, using a water bath, while for the summer-cut root systems soil temperatures were maintained at 17 °C over the growing season. In the winter-cut root systems, both log storage and root wounding caused a 40% reduction in living root mass and carbohydrate reserves, as well as reducing sucker numbers and their growth performance. In the summer-cut root systems log storage and root wounding reduced living root mass by approximately 35% as well as sucker growth, but had less of an impact on the number of suckers produced.     

Spatial patterns of Douglas-fir and aspen forest expansion

Spatial patterns, rates, and density of encroaching forests into adjacent grasslands have important implications for long-term land use management and resource planning. This study examines the effects of Douglas-fir (Pseudotsuga menzeisii) and aspen (Populus tremuloides) regeneration mechanisms on sucker and seedling spatial patterns, distance from adult trees, and density in encroaching forests. A total of 8,924 aspen suckers and 1,244 Douglas-fir seedlings were counted and mapped in 2,920 quadrats (5 m × 5 m) in 106 plots along a lower forest-grassland ecotone in the Centennial Valley, MT, USA. Sucker and seedling spatial patterns were analyzed using Morisita’s I index. Average sucker and seedling density per quadrat and distance from adult trees were estimated for each plot and compared between aspen-dominated plots and Douglas-fir-dominated plots using ANOVA tests. Aspen suckers were established in a clustered spatial pattern at a significantly higher density and a significantly shorter distance from the adult trees. In contrast, Douglas-fir seedlings were established in varying spatial patterns at a significantly lower density and a significantly greater distance from the adult trees. Forest encroachment into the adjacent grassland in the Centennial Valley is occurring in contrasting patterns and at varying rates and densities due to the difference in aspen and Douglas-fir regeneration mechanisms.     

Effects of pre- and post-harvest spray with glyphosate and partial cutting on growth and quality of aspen regeneration in a boreal mixedwood forest

In the boreal forest, conifer release treatments can leave a low quality hardwood component, which does not contribute to healthy, productive mixedwood forests. In this study, the growth and quality of trembling aspen (Populus tremuloides Michx.) regeneration were examined 5-7 years after spraying with glyphosate herbicide before and after harvesting. Results were compared to those from a partial cut and clearcut treatment without herbicide treatments. The preharvest spray treatment effectively reduced aspen density and height but did not lower regeneration quality—assessed by stem and crown deformation and stem stain—compared to the postharvest spray and clearcut treatments. Increased stem stain in the postharvest spray treatment was largely associated with the stem section that grew prior to herbicide application—post-herbicide growth was not affected. While the effect of stem stain may be restricted to growth that occurred before herbicide use, stem deformation from stem dieback may have longer term effects. In the partial cut treatments both density and stocking of aspen regeneration were lower, but aspen basal diameter growth, height growth, and quality were similar to those in the clearcut treatment. Thus, preharvest spray should promote conifer growth by reducing the density and growth of aspen regeneration without reducing the quality of aspen; this should be considered a preferred treatment to support for management objectives calling for productive and healthy mixtures of fast-growing aspen and slow-growing conifers.     

Sprouting of Populus tremula L. in spruce regeneration areas following alternative treatments

We investigated the efficiency of three different methods to restrict sprouting in European aspen (Populus tremula L.) in regeneration areas of spruce in Finland. The methods compared were (1) traditional method (cutting at 10?C15 cm height) (2) cutting at 1 m height, and (3) fungus treatment (cutting at 10?C15 cm height, and mycelium of a white-rot fungus, Chondrostereum purpureum [Pers. Ex Fr.] Pouzar was applied to freshly cut stumps). The results indicated that the fungus treatment can restrict the emergence and growth of stump sprouts better than other methods, whereas cutting at 1 m seemed to yield the lowest numbers of root suckers. However, differences between the treatments were not significantly different (P values were > 0.05). For future experimentation, we suggest that the cutting of higher stumps to control the number of root suckers should be combined with application of C. purpureum to restrict the emergence and growth of stump sprouts. We also suggest developing more efficient C. purpureum isolates to control European aspen stump sprouting.     

Effects of aspen canopy removal and root trenching on understory microenvironment and soil moisture

Effects of three aspen (Populus tremuloides Michx.) canopy removal treatments and root trenching on understory microenvironment and moisture were tested at Parkland and Boreal sites in Alberta, Canada. Aspen canopies moderated air temperature by reducing maximums and increasing minimums, and increased the frost-free period in the understory by reducing radiative frosts. When daily differences were found among canopy treatments, maximum absolute humidity was greater with complete canopy removal. Maximum daily relative humidity was greater in openings at night than with either full or partial canopy cover. Predictably, increasing aspen cover reduced PAR reaching the understory. Soil moisture response was highly variable, changing with site, aspen density and precipitation patterns, but there were only marginal differences due to root trenching. In the Parkland site, soil moisture conservation from aspen canopy and leaf litter effects were masked by tree uptake in most periods, but a net increase in soil water (+5.2%) was observed during drought. Soil and microclimatic conditions in thinned aspen stands suggest potentially favourable production benefits from developing and adopting agroforestry systems in these northern ecosystems.     

Trembling aspen root suckering and stump sprouting response to above ground disturbance on a reclaimed boreal oil sands site in Alberta,Canada

New Forests - Trembling aspen (Populus tremuloides Michx.) is an important early successional species in the boreal region that commonly regenerates via root suckering and, to a lesser extent,...     

Microclimatic and spruce growth gradients adjacent to young aspen stands

Effective utilization of a patch or cluster approach to growing aspen-white spruce mixtures in the boreal forest requires an understanding of how the faster growing aspen patches influence microclimate and spruce growth in adjacent spruce patches. In this paper we examine, how young (11–13 year old) aspen (Populus tremuloides Michx) patches influence microclimatic conditions in adjacent openings and how these are reflected in the growth of white spruce (Picea glauca [Moench] Voss) on three boreal mixedwood sites in west-central Alberta, Canada. Light levels increased continuously across the aspen stand boundary and reached maximum levels within the opening, while soil moisture was highest near the edge of the opening and then decreased with distance into the aspen stand or into the adjacent opening north of the aspen stand. Light levels were reduced over a greater distance when moving north from aspen stand edges compared to openings located south of the young aspen. These young aspen stands provided growing season frost protection within one tree length from the edge. The growth of white spruce was positively related to initial crown size and either light levels or distance from the edge. Stem volume growth was best predicted by initial tree size and the light levels at the midcrown of seedlings.     

Does mechanical site preparation affect trembling aspen density and growth 9–12 years after treatment?

Trembling aspen (Populus tremuloides Michx.) density and growth were assessed 9–12 years after stand establishment to determine whether mechanical site preparation (MSP) affects crop tree quality. Study sites were either treated with disc trenching or ripper ploughing and planted with white spruce (Picea glauca (Moench) Voss) seedlings immediately after harvest (treated) or were undisturbed since harvest (control). Stands were surveyed during the summer of 2002 with standard regeneration survey plots. Results show that aspen stem density and height were lower in MSP-treated areas relative to untreated areas. Diameter growth rates were unaffected by treatment, however the percentage of stem discolouration was higher in untreated control stands compared to site prepared areas. The results of this study indicate that there are no long-term benefits to carry out MSP for aspen promotion. However, as MSP does not appear to seriously harm the aspen crop, we suggest that this treatment can still be used on sites where aspen densities may be low without treatment (e.g., sites with extremely low soil temperatures, poor soil aeration, or vigorous competitive vegetation) or where a mixture of aspen and planted spruce are desired.     

Spring frost and decay fungi are implicated in suppressing aspen re-growth following partial cleaning in juvenile stands

? Aspen (Populus tremuloides Michx.) regenerates at high densities following manual cleaning.

? Ten-year-old stands located near Lac La Biche and Peace River, Alberta were manually cleaned to three densities (0, 500 or 1 500 stems ha^?1) at three times (bud set, dormancy or bud flush) to test the hypothesis that maintaining residual aspen reduces regeneration.

? At Lac La Biche up to 98% of the aspen regeneration died in the partially-cleaned plots compared to 67% at Peace River five years post-treatment. A spring frost in the second growing season at Lac La Biche is hypothesized to be the inciting factor predisposing the stump sprouts to infection by decay fungi such as Armillaria root rot, resulting in reduced density and height of the aspen regeneration at Lac La Biche relative to Peace River. Drought and ungulate herbivory provided additional stresses. The high mortality of aspen regeneration at Lac La Biche shifted the understory regeneration from aspen to balsam poplar (Populus balsamifera L.).

? These results indicate that maintaining 1 500 stems ha^?1 of residual aspen will not effectively control the re-sprouting of aspen; however, the vulnerability of aspen regeneration to spring frost and other stressors can nearly eradicate the re-growth of aspen.

     

Will changes in root-zone temperature in boreal spring affect recovery of photosynthesis in Picea mariana and Populus tremuloides in a future climate?

Future climate will alter the soil cover of mosses and snow depths in the boreal forests of eastern Canada. In field manipulation experiments, we assessed the effects of varying moss and snow depths on the physiology of black spruce (Picea -mariana (Mill.) B.S.P.) and trembling aspen (Populus tremuloides Michx.) in the boreal black spruce forest of western Québec. For 1 year, naturally regenerated 10-year-old spruce and aspen were grown with one of the following treatments: additional N fertilization, addition of sphagnum moss cover, removal of mosses, delayed soil thawing through snow and hay addition, or accelerated soil thawing through springtime snow removal. Treatments that involved the addition of insulating moss or snow in the spring caused lower soil temperature, while removing moss and snow in the spring caused elevated soil temperature and thus had a warming effect. Soil warming treatments were associated with greater temperature variability. Additional soil cover, whether moss or snow, increased the rate of photosynthetic recovery in the spring. Moss and snow removal, on the other hand, had the opposite effect and lowered photosynthetic activity, especially in spruce. Maximal electron transport rate (ETR(max)) was, for spruce, 39.5% lower after moss removal than with moss addition, and 16.3% lower with accelerated thawing than with delayed thawing. Impaired photosynthetic recovery in the absence of insulating moss or snow covers was associated with lower foliar N concentrations. Both species were affected in that way, but trembling aspen generally reacted less strongly to all treatments. Our results indicate that a clear negative response of black spruce to changes in root-zone temperature should be anticipated in a future climate. Reduced moss cover and snow depth could adversely affect the photosynthetic capacities of black spruce, while having only minor effects on trembling aspen.     

Effect of stock type characteristics and time of planting on field performance of aspen (Populus tremuloides Michx.) seedlings on boreal reclamation sites

Aspen (Populus tremuloides Michx) has great potential as a reclamation species for mining sites in the boreal forest, but planting stock has shown poor field performance after outplanting. In this study we tested how different aspen seedling characteristics and planting times affect field outplanting performance on reclamation sites. We produced three different types of aspen planting stock, which varied significantly in seedling size, root-to-shoot ratio (RSR), and total non-structural carbohydrate (TNC) reserves in roots, by artificially manipulating shoot growth during seedling production. All three stock types were then field-planted either in late summer, late fall, or early spring after frozen storage. Seedlings were outplanted onto two reclaimed open-pit mining areas in the boreal forest region of central and east-central Alberta, Canada, which varied significantly in latitude, reclamation history, and soil conditions. Overall, height growth was better in aspen stock types with high RSR and TNC reserves. Differences in field performance among aspen stock types appeared to be more strongly expressed when seedlings were exposed to more stressful environmental site conditions, such as low soil nutrients and moisture. Generally, aspen seedlings planted with leaves in the summer showed the poorest performance, and summer- or fall-planted seedlings with no shoot growth manipulation had much greater stem dieback after the first winter. This indicates that the dormancy and hardening of the stem, as a result of premature bud set treatments, could improve the outplanting performance of aspen seedlings, particularly those planted during summer and fall.     

Regeneration behavior of competing plants after clear cutting: implications for vegetation management

Regeneration characteristics and population dynamics of four major competing plants, trembling aspen (Populus tremuloides Michx.), pin cherry (Prunus pensylvanica L.f.), green alder (Alnus viridis spp. crispa (Aiton) Turril) and beaked hazel (Corylus cornuta Marsh.) were studied from a seven-year-old clearcut in northwestern Ontario, Canada. The site was planted with jack pine two years after clearcutting. Regeneration strategies and population dynamics of these plants were studied by determining their crown diameter, stem density, stem height, stem age, depth of sprouting center, inter-sprout distance, oven-dry weight of shoots, roots plus rhizomes by nondestructive and destructive sampling. Stem density of trembling aspen and pin cherry in 1992 was 4580 and 3600 stems per ha respectively. Much higher stem density was obtained in green alder and beaked hazel during the same time, 27580 and 14600 stems per ha respectively. Substantial reduction in stem density was recorded in trembling aspen (45%) and pin cherry (69%) over two years, 1992–1994. However, reduction in stem density of green alder and beaked hazel for that period was very little (6 and 2%, respectively). Comparison of species' clonal characteristics of above- and below-ground components indicates that trembling aspen and pin cherry possess similar vegetative regeneration strategies that differ from those of green alder and beaked hazel. Ordination of the results of canonical variate analysis of the eight vegetative parameters of the four species arranged the species into two significantly different groups. Based on species regeneration strategies, two potential competition strategies were identified: a vertical competition strategy (VCS) and a horizontal competition strategy (HCS). We argue that the degree and duration of competition can be predicted from the density and ratio of VCS and HCS plants on a site once sufficient empirical data on the species' competitive abilities are gathered. We suggest that future studies should relate the regeneration strategies, population dynamics and competitive abilities of competing plants to competition tolerance of crop trees. This will fine tune our prediction about species interaction based on the present model and better justify the need for vegetation control intervention.     

The influences of conifer succession, physiographic conditions and herbivory on quaking aspen regeneration after fire

Fire suppression over the last century has increased conifer expansion and dominance in aspen-conifer forests, which appears to be a driving force behind aspen decline in some areas. The primary objective of this study was to examine how increasing conifer dominance affects aspen regeneration vigor following the return of fire. The influence of physiographic features and herbivory on aspen regeneration vigor were also examined. The study was conducted in the Sanford fire complex located in the Dixie National Forest in southern Utah, USA, where more than 31,000 hectares burned in the summer of 2002. Seven years after the burn (at 66 locations) we measured aspen regeneration density and height as response variables and former stand composition and density (the burned trees were still standing), soil characteristics, slope, aspect and presence or absence of herbivory as independent variables. Aspen regeneration (root suckering) densities ranged from <500 to 228,000 stems/hectare with an average of 37,000 stems/hectare. Post-fire aspen regeneration density was most strongly correlated with pre-fire stand successional status (as measured by stand composition and species abundance), with percent conifer abundance (R² = −0.55) and overstory aspen density (R² = + 0.50) being the most important. Average aspen suckering densities ranged from approximately 60,000 stems/hectare in what were relatively pure aspen stands (>90% aspen) to less than 5000 stems/hectare in stands where conifer abundance was greater than 90%. Soil C, N, and P showed positive correlations (R² = 0.07 to 0.17) with aspen regeneration vigor, while soil texture had a relatively weak influence on sucker regeneration. Aspen regeneration densities were 15% lower on north facing aspects compared to east, west and south facing aspects with slope steepness showing no correlation with regeneration vigor. Regeneration density was significantly lower (8%) at sites with evidence of herbivory versus sites where herbivory was absent. Overall, the aspen regeneration response in the Sanford fire complex was strong despite high wildlife densities, which may be related to disturbance size. Where the maintenance of aspen is desired in the landscape we recommend promoting fire when the percentage of overstory conifer stems is greater than 80% or overstory aspen density is less than 200 overstory stems/hectare.     

Aspen canopy removal and root trenching effects on understory vegetation

Effects of aspen (Populus tremuloides Michx.) canopy removal and root trenching on understory vegetation were examined at Parkland and Boreal sites in Alberta, Canada to provide a better understanding of the ecological basis of agroforestry systems suitable for north temperate and boreal areas. The greatest changes in understory production (ANPP) were in response to canopy removal with less consistent changes from reduction of root effects. Root trenching did not influence ANPP at the parkland site, but did at the boreal site where it interacted with canopy removal. During severe drought, ANPP under a full canopy at the parkland site increased and may relate to paradoxical understory resource increases during drought. At the parkland site there was a shift away from forb and shrub production under a full canopy towards a greater proportion of graminoids with complete or partial canopy removal. At the boreal site, partial canopy removal resulted in more shrubs and greater production from graminoid species relative to complete overstory removal but only with root trenching using a barrier. Trends in the relative yields of understory species and the cover of plant groups generally followed the patterns observed in ANPP. Leaf litter decreased with the level of canopy removal and may have factored in greater moisture conservation under aspen. Understory production gains with a partial canopy may be attributed to favourable microclimatic conditions of a closed forest retained by the partial aspen cover, while simultaneously increasing PAR penetration. Soil resources were important to ANPP and cover, particularly among forbs, however, when root trenching was significant, it generally coincided with increases in PAR.     

Development and activity of early saproxylic fungal communities in harvested and unmanaged boreal mixedwood stands

Limited scientific information is currently available regarding saproxylic fungal communities in the boreal forest of North America. We aimed to characterize the community development, richness and activity of saproxylic fungi on fresh wood in harvested and unmanaged boreal mixedwood stands of northwestern Québec (Canada). Fresh wood blocks (n = 480) of balsam fir (Abies balsamea (L.) Mill.) and trembling aspen (Populus tremuloides Michx.) were placed on the forest floor in a range of stand conditions (n = 24). Blocks were harvested every 6 months for up to 30 months and characterized for species composition and richness (PCR–DGGE, DNA sequencing), respiration, wood density and lignin and cellulose content. Colonization by a wide range of functional groups proceeded rapidly under different stand conditions. We detected a total of 35 different fungal operational taxonomic units, with the highest species richness at the wood block level being observed within the first 12 months. No differences in community composition were found between wood host species or among stand conditions. However, the variability in fungal communities among blocks (β diversity) was lower on trembling aspen wood compared with balsam fir and decreased over time on trembling aspen wood. Also, fungal activity (respiration and wood decomposition) increased on trembling aspen wood blocks and species richness decreased on balsam fir wood over time in partial-cut sites. The overlap in tree composition among stands, the high volume of logs and the recent management history of these stands may have contributed to the similarity of the saproxylic fungal community among stand types and disturbances.