The influence of challenging objects and horse-rider matching on heart rate, heart rate variability and behavioural score in riding horses

A good horse-rider 'match' is important in the context of equine welfare. To quantify the influence of repetition and horse-rider matching on the stress of horses encountering challenging objects, 16 Warmblood horses were ridden in a test-setting on three occasions. On each occasion the horse was ridden by a different rider and was challenged by three objects (A-C). Heart rate (HR), heart rate variability (HRV) of horse and rider, and behaviour score (BS) of the horse were obtained for each object and as a total for each test. The horse-rider interaction was evaluated with each combination and assessed as 'matching' or 'mismatching', and the horses were categorised as 'compliant', 'partly-compliant' or 'non-compliant'. Horses exhibited a decreased HR (P=0.015) and a decreased BS (P=0.004) within and across different tests. 'Matching' horse-rider combinations exhibited less stress as indicated by reduced HR ('match' 69±10 vs. 'mismatch' 72±9, P=0.001) and BS ('match' 1.9±1.1 vs. 'mismatch' 3.8±1.4, P=0.017) of the horse. 'Compliant' (68±8, P<0.001) and 'partly-compliant' (71±9, P=0.002) horses had significantly lower HR than 'non-compliant' (75±9) animals. The findings of the study indicate that HR and BS measurements support a subjective 'match' diagnosis and HR measurement may be a valuable tool in assessing horse compliance.     

Optimum test period and associations between standard 140-day test period and shorter test periods for growth rate in station tested beef bulls

SUMMARY: The records of 2034 beef bulls representing 931 sire families from ten breeds tested at Ellerslie Test Station, Alberta, Canada, from 1974 to 1987 were analyzed to investigate the feasibility of selecting a shorter test period and study the associations between the growth rate in the standard 140-day test period and that in shorter test periods for accurate evaluation of growth potential of young beef bulls in test station. The results indicated that the period between day 28 and day 112 of the standard test was less affected by herd of origin compared with the standard 140-day test period and therefore could be considered as the short optimum test period. Average daily gain in this period was least affected by herd of origin, and had relatively high heritability which would ensure satisfactory selection response. In order to properly evaluate growth potential of beef bulls and economically make use of the testing facilities, it would be appropriate to have an adjustment period of 56 days (original 28 days plus the first 28-day period of the standard test) followed by a test period of 84 days (between day 28 and day 112 of the standard test). Such a test would result in reduction in management costs while providing a more accurate evaluation of growth potentials of young beef bulls. ZUSAMMENFASSUNG: Optimale Prüfperiode und Zusammenh?nge zwischen Standard-140-Tage- und kürzerer Prüfung für Wachstumsrate bei Stationsprüfung von Fleischrind-Bullen Leistungsangaben von 2034 Fleischbullen aus 931 Bullennachkommenschaften von 10 Rassen wurden auf der Allersly-Station Alberta, Kanada, von 1974 bis 1987 im Hinblick darauf analysiert, inwieweit Beziehungen zwischen Wachstumsrate in der Standard-140-Tage-Periode und der in kürzeren Zeitabschnitten existieren. Die Ergebnisse zeigen, da? die Prüfperiode zwischen den Tagen 28 und 112 des Standardtests weniger durch Herkunft tangiert wird als die Standard 140-Tagetest-Periode und daher als optimale Prüfperiode betrachtet werden kann. Durchschnittszunahmen waren in dieser Periode am wenigsten durch Herkunft beeinflu?t und haben relativ hohe Heritabilit?t, die hinreicht, um Selektionserfolg zu sichern. Um das Wachstumspotential von Fleischrindbullen zu bewerten und Testanlagen ?konomisch zu nutzen, w?re es angebracht, eine Anpassungsperiode von 56 Tagen (ursprüngliche 28 plus die ersten 28 Tage der bisherigen Testperiode) vor einer eigentlichen Testperiode von 84 Tagen erfolgen zu lassen. Hierdurch würden Managementkosten reduziert und eine genauere Sch?tzung des Wachstumspotentials der Fleischbullen gesichert sein.     

Influence of cage size on heart rate and behavior in rhesus monkeys

We studied 6 singly caged adult female rhesus monkeys to determine whether increased cage size had any effect on behavior or heart rate. Two monkeys at a time were placed in cages 40% larger than their standard cage for 1 week on 2 occasions, using a counter-balanced design. Direct behavioral observations were performed 75 minutes/week on each monkey. Heart rate and general activity were monitored 35 hours/week by a telemetry system. Statistically significant differences were not found in aggressive, submissive, abnormal, or self-abusive behavior, nor in time spent in the front half of the cage, duration of grooming, looking at the observer, or stereotyped or nonstereotyped locomotion. Vocalizations increased the first time in the larger cage, but not the second, and decreased upon the second return to the standard cage. Differences with respect to cage size were not found in heart rate or activity level, although there were significant variations at different times of day. We conclude that modest increases in cage size are unlikely to enrich the environment of singly caged laboratory primates.     

Pre-operative stress in dogs - a preliminary investigation of behavior and heart rate variability in healthy hospitalized dogs

OBJECTIVE: To assess pre-operative behavioral and physiological characteristics of healthy dogs hospitalized for elective surgery. STUDY DESIGN: Open clinical observational study. ANIMALS: Forty-one bitches hospitalized for elective ovariohysterectomy. METHODS: While undisturbed in a hospital cage, the behavior of the dog was recorded using a video camera and recorder. From the video recordings, various behavioral variables were registered. Simultaneous measurements were made on heart rates and heart rate variability (HRV) by use of an ambulatory electrocardiogram. In addition, the dog's response to human approach was noted. RESULTS: Different behavioral patterns were found in the dogs studied. Thirteen individuals were regarded as highly active, and were seen to bark or howl, manipulate the environment or attempt to flee vigorously. In 13 dogs (passive dogs) none of these activities occurred. Panting and displacement behaviors, such as snout licking, were observed in nearly all the animals monitored. In general, heart rates were higher and HRV lower with the most active individuals; however, the presence of physiological arousal could not be excluded in two animals with passive behaviors. To some extent, the behavior of the dog while undisturbed in the cage was reflected in the responses to a person entering the cage. CONCLUSIONS AND CLINICAL RELEVANCE: The different individual responses detected in this study raise an important question concerning their role and existence throughout the entire peri-operative period, especially during the post-anesthetic recovery phase when behavioral characteristics are commonly used to assess patient welfare. The results also emphasize the need for further investigations to explore the effects of pre-operative stressors on canine surgical patients, and the factors contributing to them.     

Flooring preference and behavior in sound and lame sows

The objectives of this study were to assess sow flooring preference and behavior during sound, induced lame and lameness recovery states. Thirty-four multiparous, non-pregnant sows were compared prior to and following lameness induction (day 0). Behavior was recorded on six days (days ?1, +1, +2, +3, +4 and +10). Three flooring locations (Alley, Concrete and Mat) and four behaviors (lying lateral, lying sternal, sitting and standing) were recorded. Sows increased Mat frequency and decreased Alley frequency on day +1 (most lame) relative to day ?1. Lying lateral frequency increased, whereas lying sternal and standing frequencies decreased between day ?1 and day +1. Sows appear to prefer the Mat within 24?h following lameness induction. Lying lateral is likely a more comfortable position than standing or lying sternal in these lame sows. In conclusion, these behaviors may be reliable indicators to further ascertain if a sow is lame.     

Comparison of heart rate and heart rate variability obtained by heart rate monitors and simultaneously recorded electrocardiogram signals in nonexercising horses

Heart rate (HR) and heart rate variability (HRV) are often determined with Polar heart rate monitors (HRMs; S810i; Polar, Kempele, Finland). The aims of this study were to compare data from horses obtained by Polar HRMs and a portable Televet electrocardiogram (ECG; 100 version 4.2.3; Kruuse, Marslev, Denmark) device and to determine appropriate recording times in horses (n = 14). Correlations were calculated and a Bland-Altman analysis was carried out to examine agreement between recording systems. For beat-to-beat (RR) interval, uncorrected and corrected data were highly correlated irrespective of the recording system and recording time (r > 0.99, P < 0.001). For HRV variables, standard deviation of RR interval and root mean square of successive RR intervals, correlations higher than 0.9 were obtained between uncorrected and corrected ECG but not Polar data. The RR interval, HR, and HRV from corrected Televet and Polar data at no time differed between the recording systems. However, with the increase in recording time, the RR interval decreased (P < 0.001). Thus, for comparisons, recording intervals of similar length should be chosen. Correlations among RR interval, HR, and HRV variables obtained by ECG and HRMs were highly significant at all recording times (r > 0.9, P < 0.001). Correlations increased with increasing recording time. Bland-Altman graphs showed a strong agreement between HRMs and ECG and mean RR intervals, HR, and HRV variables were close to identical. In conclusion, Polar HRMs are as adequate as ECG recordings in horses. Owing to a low HR in stationary horses, recording times below 2 minutes will underestimate changes in HR and HRV.     

Effect of manual and motorized dental rasping instruments on Thoroughbred's heart rate and behavior

Inadequate research exists to justify the choice of dental tools used in equine prophylactic dentistry; usage is unregulated, with choice usually based on the practitioner’s preference. Dental procedures could cause stress (through handling or equipment used), as they are potentially painful if contact is made with soft-tissues. Anecdotally adverse reactions are documented and differ when comparing manual with motorized rasping, particularly during rasping the lower arcade. The study aimed to investigate these claims.Forty-five Thoroughbreds were divided into groups: (A): manual rasping, (B): motorized rasping, and (C): control. Horses were accustomed to prophylactic dentistry (6-12 months previously) and required a routine float. A standardized protocol was used; heart rate (HR) and scale-graded interactive behavioral responses (BR) were recorded to evaluate subjects’ stress and pain pre-, peri-, and posttreatment.Increased HR and BR were observed in group A compared with groups B and C (P < 0.001). Motorized rasping produced a significant increase in HR (P < 0.001) and BR (P < 0.001) from the controls. The results support postulation of higher sensitivity in the lower arcades, with these arcades exhibiting significantly higher HR and BR in both motorized and manual groups (P < 0.001). “Bit-seating” recorded higher responses (HR and BR: P < 0.001) compared with routine rasping.The study could inform routine dentistry; motorized rasping produced less-reactive horses, potentially providing a safer working environment for dental technicians and increased welfare for their equine patients.     

Changes in heart rate and heart rate variability in Thoroughbreds during prolonged road transportation

OBJECTIVE: To determine whether evaluation of heart rate (HR) and HR variability (HRV) during prolonged road transportation in horses provides a sensitive index of autonomic stimulation. ANIMALS: Five 2-year-old Thoroughbreds. PROCEDURE: ECGs were recorded as horses were transported for 21 hours in a 9-horse van. Heart rate, high-frequency (HF) power, low-frequency (LF) power, and LF-to-HF ratio from Fourier spectral analyses of ECGs were calculated and compared with values recorded during a 24-hour period of stall rest preceding transportation. RESULTS: HR, HF power, and LF power had diurnal rhythms during stall rest but not during road transportation. Heart rate was higher and HF power and LF power lower during road transportation than stall rest, and HR, HF power, LF power, and LF-to-HF ratio all decreased with time during road transportation. Heart rate during stall rest was weakly and inversely associated with LF power, but during road transportation was strongly associated with LF power, HF power, and LF-to-HF ratio. Neither LF power nor HF power was correlated with LF-to-HF ratio during stall rest, but LF power was strongly and HF power weakly correlated with LF-to-HF ratio during road transportation. High-frequency power and LF power were significantly correlated with each other during stall rest and road transportation. Heart rate was significantly influenced by LF power and LF-to-HF ratio during stall rest (R(2) = 0.40) and by HF power and LF-to-HF ratio during road transportation (R(2) = 0.86). CONCLUSIONS AND CLINICAL RELEVANCE: HR is influenced by different sympathovagal mechanisms during stall rest, compared with during road transportation; HRV may be a sensitive indicator of stress in transported horses.     

Standardized exercise test and daily heart rate responses of thoroughbreds undergoing conventional race training and detraining

Ten healthy sedentary male Thoroughbreds with previous race training experience were studied for 14 weeks. Horses were trained for 9 weeks, using a program designed after those used commonly in the United States. Horses were trained conventionally by slow trotting (250 m/min) for 2 weeks and galloping (390 to 450 m/min) for 4 weeks, followed by 3 weeks of galloping (440 to 480 m/min) and intermittent sprinting exercises (breezes) at distances between 600 and 1,000 m (900 to 950 m/min). The horses were then pasture rested for 5 weeks. A standardized exercise test (SET) involving an 800-m gallop at 800 m/min was administered before and after the 9-week training period and after the 5-week detraining period. Heart rate (HR) was monitored during exercise and at standardized intervals after exercise for 60 minutes. Venous blood for determination of plasma lactate concentration was obtained at 5 minutes after exercise. Heart rate was monitored daily at rest, during exercise, and through the first 60 minutes of recovery. Venous plasma samples (for lactate determination) were obtained 5 minutes after the sprinting exercises. Horses were observed daily before exercise for signs of lameness and were not allowed to train if lame. Differences after 9 weeks' training were seen in the SET recovery HR at 0.5 through 5 minutes after exercise (P less than 0.05 to P less than 0.01). Differences after detraining were seen in the SET recovery HR at 40 and 60 minutes after exercise (P less than 0.05 to P less than 0.01). Neither training nor detraining resulted in differences in plasma lactate concentration after the SET gallop.(ABSTRACT TRUNCATED AT 250 WORDS)     

Preliminary results suggest an influence of psychological and physiological stress in humans on horse heart rate and behavior

People may involuntarily emit fear or distress signals when around horses, and interpreting how horses respond to these messages is important, particularly for human safety around horses. No studies have been done to determine if horses can differentiate between humans who are physiologically stressed (e.g., after exercising) as opposed to psychologically stressed (e.g., afraid). Horses (N = 10) loose in a round pen were randomly subjected to the presence of a stationary blindfolded human in each of 4 treatments: (1) calm human comfortable around horses (CALM), (2) physically stressed human (PHYS; exercised to 70% maximum heart rate [HR]), (3) psychologically stressed human (PSYCH; afraid of horses), or (4) no human (CONTROL). Both humans and horses were equipped with an HR monitor. Physiological and behavioral observations (gait, head position relative to the withers, distance and orientation toward human) were recorded and analyzed using a mixed model with horse and human as random effects. Increasing human fearfulness was associated with a decrease in horse HR (P = 0.0156). Horses moved at a slower gait in PSYCH (P < 0.0001), and horse head position was lower during PHYS and PSYCH compared with CALM or CONTROL (P < 0.0001). Human HR was highest in PHYS (P < 0.0001) and decreased over time in all treatments. Human HR increased when the horse was facing away (P = 0.0395). Overall, horses appear less stressed in the presence of a stationary fearful or physically stressed human than a calm person. Thus, horses in the presence of fearful humans, particularly where participants may not be comfortable around horses, should not pose any additional risk provided normal safety precautions are used.     

The effect of relaxing massage on heart rate and heart rate variability in purebred Arabian racehorses

The objective of this study was to assess the effect of relaxing massage on the heart rate (HR) and heart rate variability (HRV) in young racehorses during their first racing season. In the study, 72 Purebred Arabian racehorses were included. The study was implemented during the full race season. The horses from control and experimental groups were included in regular race training 6 days a week. The horses from the experimental group were additionally subject to the relaxing massage 3 days a week during the whole study. HR and HRV were assumed as indicators of the emotional state of the horses. The measurements were taken six times, every 4‐5 weeks. The HRV parameters were measured at rest, during grooming and saddling the horse and during warm‐up walking under a rider. The changes of the parameters throughout the season suggest that the relaxing massage may be effectively used to make the racehorses more relaxed and calm. Moreover, the horses from the experimental group had better race performance records.     

Influence of sickness condition on diurnal rhythms of heart rate and heart rate variability in cows

Parameters of heart rate variability would explain changes in heart rate during the disease status in cows and to evaluate whether such changes might provide a more sensitive and quantitative indicator of these conditions than crude indices. For this purpose, we recorded electrocardiograms for 24 hr using a Holter-type electrocardiograph and applied power spectral analysis of heart rate variability in both five clinically healthy and four hospitalized cows. The significant findings of the current investigation were that the diurnal variations of autonomic nervous function are abolished in cows that are sick. This abnormal rhythm was induced by predominant parasympathetic inhibition in these cows. Therefore, the heart rate variability may be a useful indicator of sickness condition in cows.     

Effects of epidural opioid analgesics on heart rate, arterial blood pressure, respiratory rate, body temperature, and behavior in horses

Heart rate, arterial blood pressures, respiratory rate, body temperature, and central nervous system excitement were compared before and after epidural administration of morphine (0.1 mg/kg), butorphanol (0.08 mg/kg), alfentanil (0.02 mg/kg), tramadol (1.0 mg/kg), the k-opioid agonist U50488H (0.08 mg/kg), or sterile water using an incomplete Latin square crossover design in five conscious adult horses. Treatments were administered into the first intercoccygeal epidural space. Significant (P <.05) reductions in respiratory rate were detected after epidural administration of morphine, alfentanil, U50488H, and sterile water. Additionally, significant (P <.05) head ptosis was observed within the first hour after administration of morphine, U50488H, and tramadol, but neither of these changes appeared to be of clinical significance. No treatment-related changes in motor activity or behavior were observed.