河南省正阳县三元仔猪繁育基地

我处是县委、县政府、县畜牧局等相关部门重点扶持仔猪、种猪繁育基地。常年供应纯正三元（杜长大）杜洛克、大约克等良种、良杂瘦肉型仔猪。另售新台系杜洛克、新丹系长白、加系大约克种猪及二元母猪。以上仔猪、种猪由畜牧局防疫专家定时防疫，售后跟踪服务。并特配抗应激药物，途中无应激，到家不拉稀，常年出售商品猪。     

新加系种猪繁殖及其子代生长性能分析

旨在统计分析新加系种猪的繁殖性能及其子代的生长性能。对正常生产的新加系种猪1胎和2胎分娩后24 h内的总产仔数、产活仔数、仔猪初生窝重等繁殖性状进行统计,同时对新加系种猪生产的第1批子代初生重、21日龄个体重、达65 kg体重日龄、达120 kg体重日龄、120 kg体重背膘厚和眼肌厚以及采食量等生长性状测定统计,并对结果进行了差异分析。结果显示：大约克母猪第1胎的平均总产仔数和平均产活仔数均极显著高于杜洛克母猪(P<0.01),长白母猪第1胎的平均总产仔数和平均产活仔数均极显著高于杜洛克母猪(P<0.01);大约克母猪第2胎的平均总产仔数和平均产活仔数均极显著高于长白母猪和杜洛克母猪(P<0.01),长白母猪第2胎的平均总产仔数和平均产活仔数均极显著高于杜洛克母猪(P<0.01);大约克母猪第2胎的平均总产仔数和平均产活仔数均极显著高于第1胎(P<0.01);杜洛克母猪第2胎的平均总产仔数显著高于第1胎(P<0.05),平均产活仔数极显著高于第1胎(P<0.01)。子代生长性能,大约克公猪的平均日采食量显著高于长白公猪(P<0.05),...     

信息快递

全国各省市主要种猪场种猪销售价格　(2 0 0 2年 10月 15日 )1河南省正阳县示范种猪场2 0 0 1年从国外引进纯种猪 :新加系大约克、新丹系长白、台系杜洛克 :14 0 0～ 2 0 0 0元 /头 ;新丹加二元杂交母猪 :90 0元 /头。以上均为 5 0ｋｇ体重　　场长 :张　汀　　手机 :13 93 83 9172 4联系人 :凌礼振　雷明生　电话 :0 3 96-883 3 0 972中西合资邢台市齐发牧业有限公司邢台市种猪育种中心台系杜洛克 :公、母猪 13 0 0元 /头 ;比利时长白 :公、母猪 13 0 0元 /头 ;新英系大约克 :公、母猪 13 0 0元 /头 ;二元长大 :母猪80 0元 /头。以上均为 5…     

引进美国SPF杜洛克种猪生产性能测定

选用引入的SPF杜洛克种猪76头，经过近三年的饲养观察及生产性能测定，达到原产地美国杜洛克种猪的生产性能，保持了原种猪的优良特性。与本场饲喂的大约克种猪生产性能比较，结果表明生长速度与胴体瘦肉率明显优于大约克猪，可作优良的瘦肉型猪终端父本。     

全国各省市主要种猪场种猪销售价格

河南省汝南外贸种猪场台系杜洛克 :公 160 0元 /头 ;英系大约克公母、丹系长白公母 :14 0 0元 /头 ;长大二元 :95 0元 /头 (体重 60ｋｇ左右 )。电话 :0 3 96-81983 88　场长 :张元发 13 90 3 960 2 602河南省正阳县示范种猪场2 0 0 1年从国外引进纯种猪 :新加系大约克、新丹系长白、台系杜洛克 :15 0 0～ 2 5 0 0元 /头 ;新丹加二元杂交母猪 :90 0元 /头。以上均为 5 0ｋｇ体重　　场长 :张　汀　　手机 :13 90 3 963 0 0 9联系人 :凌礼振　雷明生　电话 :0 3 96-883 3 0 973河南省种猪育种中心新丹系长白 :特级 (公、母 ) 2 50 0元 /头 ,…     

种猪场种猪销售价格

广西农垦永新畜牧有限公司加系杜洛克:公母2500元/头;加系长白:公母2500元/头;加系大约克:公母2500元/头;新丹系大约克:公母3500元/头;新丹系长白:公母3500元/头,二元杂母猪:1400元/头。以50kg为标准,超过50kg部分,每千克10元。电话:0771-73506207350177(兼传真)联系人:唐荣福湖北省农科院畜牧兽医研究所瘦肉猪育种中心大约克:公3000元/头,母2600元/头;杜洛克:公3400元/头;长大杂:母1300元/头。种母猪体重以50kg/头为标准,头平体重每增减1kg,增减人民币8元。联系人:周军电话:(027)8798146713807161205北京资源亚太种猪有限公司品种特级公、…     

抗病育种技术在引进美国SPF种猪后代选育中应用探究

为探索利用现代分子育种技术培育抗病新品系种猪,本试验对引进美国的44头杜洛克、138头大约克、28头长白纯种猪进行耳组织采样,采用基因检测技术分别进行氟烷基因（抗应激基因）、抗腹泻基因及KPL2基因（精子短尾基因）检测。结果表明,1）氟烷基因检测：杜洛克种猪检测出氟烷基因型Nn个体（应激易感基因个体）1头,占2.33%（1/44）;大约克种猪检测出氟烷基因型Nn个体2头,占1.45%（2/138）;长白种猪未检测到氟烷基因型Nn个体（0/28）,所检测的28头猪全部为氟烷基因型NN个体（抗应激基因个体）。2）抗腹泻基因检测：杜洛克种猪群中含有抗腹泻GG基因型个体37头,占杜洛克猪检测总数的86.05%（37/43）;长白种猪群中腹泻抗性有利GG基因型个体有11头,占40.74%（11/27）;大约克种猪腹泻抗性有利基因个体有23头,占16.79%（23/137）,3个品种中杜洛克种猪群占有腹泻抗性个体最多,其次是长白种猪,大约克种猪最少。3）KPL2基因检测：所检测的138头大约克种猪,没有检出携带精子短尾遗传疾患的个体。在种猪繁育中,只要加大世代有利纯合抗性个体的选留比例,最终可以建立纯系杜洛克、大约克、长白种猪等抗应激、抗腹泻、无精子短尾症等专门化新品系。     

种猪场种猪销售价格

广西农垦永新畜牧有限公司加系杜洛克:公母2500元/头;加系长白:公母2500元/头;加系大约克:公母2500元/头;新丹系大约克:公母3500元/头;新丹系长白:公母3500元/头,二元杂母猪:1400元/头。以50kg为标准,超过50kg部分,每千克10元。电话:0771-73506207350177(兼传真)联系人:唐荣福湖北省农科院畜牧兽医研究所瘦肉猪育种中心大约克:公3000元/头,母2600元/头;杜洛克:公3400元/头;长大杂:母1300元/头。种母猪体重以50kg/头为标准,头平体重每增减1kg,增减人民币8元。联系人:周军电话:(027)8798146713807161205北京资源亚太种猪有限公司品种特级公、…     

引进美国原种猪的生产性能观察

2003年底,广西柯新源原种猪有限责任公司直接从美国SPF猪场引进美系原种猪268头,其中杜洛克公猪16头,8个血统,母猪45头;长白公猪21头,10个血统,母猪102头;大约克公猪12头,6个血统,母猪72头。种猪在美国隔离60天后空运抵达上海浦东并在上海隔离检疫45天。种猪抵达公司后经过严格、细致的饲养管理,原种一代种猪表现出优良的生产性能,现就各项性能逐一介绍。     

信息快递

种猪场种猪销售价格广西农垦永新畜牧有限公司加系杜洛克:公母2500元/头;加系长白:公母2500元/头;加系大约克:公母2500元/头;新丹系大约克:公母3500元/头;新丹系长白:公母3500元/头,二元杂母猪:1400元/头。以50kg为标准,超过50kg部分,每千克10元。电话:0771-73506207350177(兼传真)联系人:唐荣福四川省原种猪场加系长白:公优良750元/头,特优3500元/头,母优良1500元,特优3000元;大约克:公优良1000元,特优3500元,母优良2000元,特优3000元;杜洛克:公优良2000元,特优3500元,母优良2000元,特优3000元。台系长白:公优良3500元/头,特优7000元/头,母…     

高钙饲粮对青年蛋鸡血清一氧化氮浓度和抗氧化功能的影响

本试验旨在研究高钙饲粮对青年蛋鸡血清一氧化氮（ＮＯ）浓度和抗氧化功能的影响。将 ３５日龄 １００羽伊沙蛋鸡随机均分为对照组（含钙 １．００％）和高钙组（含钙 ３．７８％），每组 ５个重复，每个重复 １０羽。每天观察鸡的精神状态、食欲和粪便情况。在饲喂高钙饲粮第 ０、８、１６、２４和 ３２天时，分别测定 ２组鸡的血清中 ＮＯ浓度和丙二醛含量及抗氧化酶系统的变化。结果表明：１）高钙饲粮能引起青年蛋鸡长期腹泻，在饲喂高钙饲粮的第 ８天就发生高血钙症。２）与对照组相比，高钙组在饲喂高钙饲粮第 ８天和第 １６天血清 ＮＯ浓度无显著变化（Ｐ＞０．０５），第２４天和第 ３２天显著升高（Ｐ＜０．０５）；第 ８天和第 ２４天血清超氧化物歧化酶活性、总抗氧化能力无显著变化（Ｐ＞０．０５），第 １６天显著升高（Ｐ＜０．０５），第 ３２天显著降低（Ｐ＜０．０５）；第 ８、１６、２４天血清丙二醛含量无显著变化（Ｐ＞０．０５），第 ３２天显著升高（Ｐ＜０．０５）；第 ２４天和第 ３２天黄嘌呤氧化酶活性有升高趋势（Ｐ＞０．０５）。结果提示，饲喂高钙饲粮可导致青年蛋鸡发生长期腹泻，引起高血钙症，导致其机体内自由基的产生与清除的动态平衡遭到破坏，使自由基的产生远远超出清除能力。     

新生湖羊羔激素调节的特点和发育变化

选择１２只自然分娩，初生重１．５～４．５ｋｇ的新生湖羊羔，研究７日龄产后期内激素调节的特点和发育变化。结果表明：羔羊生后１ｈ血浆Ｃｏｒｔ、Ｔ＿４、Ｔ＿３水平均较高，而Ｉｎｓ较低。在生后１２ｈ内，血浆Ｃｏｒｔ处于较高水平，然后逐渐下降，５日龄后相对稳定；血浆Ｔ＿４、Ｔ＿３１２ｈ内明显下降，然后Ｔ＿４进一步波动性下降，Ｔ＿３回升，３日龄后Ｔ＿４、Ｔ＿３相对稳定。血浆Ｉｎｓ在生后８ｈ内呈升高趋势，７日龄内保持在较高水平上波动。     

Pharmacokinetic analysis of plasma concentration and urinary excretion of sulphamonomethoxine and its metabolite in Goettingen minipigs

The plasma concentration and renal excretion after a bolus intravenous injection of a low (10 mg/kg) or high (100 mg/kg) dose of sulphamonomethoxine (SMM) were studied in five Goettingen minipigs. The time data of plasma concentration after a low dose decreased rapidly and appeared to be linear on semilog graph paper. On the other hand, a decrease in the plasma concentration after a high dose was slow at first, gradually accelerated, then became rapid, showing that SMM disposition after a high dose (100 mg/kg) seemed to be non-linear with capacity-limited elimination. A large amount of the acetyl derivative of SMM (AcSMM), which was determined to be the main excretory product of SMM in urine, was detected in the plasma after SMM injection. As the ratios of the area under plasma concentration-time curve of AcSMM to that of SMM were not significantly different at either dose, the acetylation of SMM may be unsaturable by injection of 100 mg/kg of SMM. Immediately after the injection of a low dose, a rapid hyperbolic increase of the fraction of the cumulative amount of the excretory products in urine was observed. On the other hand, the fraction curve at the high dose rose slowly at first, then rapidly and hyperbolically. These results suggested that the non-linear drug disposition after a high dose (100 mg/kg) of SMM in pigs may be the result of a limited capacity for renal excretion of SMM and excretory products, especially the acetyl derivative.     

Differences in triglyceride and cholesterol metabolism and resistance to obesity in male and female vitamin D receptor knockout mice

A lean phenotype has been detected in vitamin D receptor (VDR) knockout mice; however, the gender differences in fat metabolism between male and female mice both with age and in response to a high‐fat diet have not been studied before. The objective of our study was to assess changes in body and fat tissue weight, food intake and serum cholesterol and triglyceride in VDR knockout mice from weaning to adulthood and after a challenge of adult animals with a high‐fat diet. Although VDR knockout mice of both sexes consumed more food than wild‐type and heterozygous littermates, their body weight and the weight of fat depots was lower after 6 months on a diet with 5% crude fat content. When adult animals were challenged with a high‐fat diet containing 21% crude fat content for 8 weeks, VDR knockout mice of both sexes had a significantly higher food intake but gained less weight than their wild‐type littermates. Cholesterol levels were higher after 2 days on the high‐fat diet in both sexes, but in the VDR knockout mice, less cholesterol was detected in the serum after 8 weeks. Wild‐type male mice showed signs of fatty liver disease at the end of the experiment, which was not detected in the other groups. In conclusion, lack of the VDR receptor results in reduced fat accumulation with age and when adult mice are fed a high‐fat diet, despite a higher food intake of VDR knockout mice relative to their wild‐type littermates. These effects can be detected in both sexes. Wild‐type male mice react with the highest weight gain and cholesterol levels of all groups and develop fatty liver disease after 8 weeks on a high‐fat diet, while male VDR knockout mice appear to be protected.     

Plasma prolidase activity as a possible diagnostic index of chronic hepatic abscess in cattle

The usefulness of prolidase as a biochemical parameter to represent the chronic state of hepatic abscess was discussed in eight cattle experimentally inoculated with Fusobacterium necrophorum and 18 spontaneously affected cattle. Blood was daily collected to measure the plasma prolidase activity and sialic acid level from the experimental cattle for 90 days after inoculation. In three out of four cattle affected with hepatic abscess, prolidase activity began to rise about 40 days after inoculation, and maintained high activity till 90 days. In the same cattle the sialic acid concentration increased from 7 to 10 days after inoculation, and gradually returned to the normal value 50 days after it. Another cow showed a similar change in early stage of experiment, but prolidase activity decreased after 70 days and sialic acid concentration maintained high level till 90 days. In two cattle, which showed scars but no abscess on autopsy, the prolidase activity increased temporarily from 40 to 55 days after inoculation. In the control cattle inoculated with an inactivated bacterial suspension, neither the sialic acid level nor the prolidase activity showed any large variation in the experimental period. Among the spontaneously affected cattle, those with a high sialic acid level revealed normal prolidase activity and those with a normal sialic acid level had high prolidase activity.     

Effect of single bouts of moderate and high intensity exercise and training on equine peripheral blood neutrophil function

The effects of single bouts of moderate (30 to 40 per cent VO(2)max) and high (115 per cent VO(2)max) intensity exercise on equine peripheral blood leucocyte function were evaluated by determining neutrophil phagocytosis and oxidative burst activity before and after treadmill exercise and training. Prior to all exercise tests, the possible effect of diurnal variation was evaluated in samples obtained from four resting horses. Subsequently eight horses underwent moderate and high intensity exercise protocols and then commenced a 17-week training period. High intensity exercise tests were repeated in week 10, after 7 weeks of endurance training, and in week 17, after a further 6 weeks of high intensity training. Time of sampling had a significant effect on neutrophil function for resting, untrained horses. Prior to training, moderate intensity exercise was associated with improved neutrophil phagocytosis and oxidative burst activity. High intensity exercise was associated with transient impairment of these responses. A similar reduction was not demonstrable following high intensity exercise in weeks 10 or 17 of training. Neutrophil function in week 17 was suppressed at all sampling times relative to results obtained in week 10, suggesting that high intensity training may have been associated with a general reduction in neutrophil function.     

Fowl cholera vaccination of growing turkeys with CU strain via routes other than oral

Turkeys developed a high level of protective immunity and serum anti-Pasteurella multocida antibody when vaccinated with the Clemson University (CU) strain of P. multocida via the following routes: wing-web puncture with the recommended dosage or 1:10 dilution of this dosage, subcutaneous, crop injection, cloacal perfusion of the bursa of Fabricius, and intratracheal. The development of immunity after vaccination via the wing-web puncture and subcutaneous routes was dose-dependent: the lower dosages induced less serum antibody and protective immunity. Immunity was greatest 3 weeks after vaccination via wing-web puncture, although immunity was evident as early as 4 days after vaccination. A high level of antibody and protective immunity with no adverse reactions was induced by vaccinating twice, either by the drinking water followed by a similar dosage parenterally or by a parenteral route followed by the drinking water; these regimens were similar to one vaccination in the drinking water. Vaccination via wing-web puncture and possibly subcutaneous injection and bursal perfusion could be used for flocks with a history of a high mortality after vaccination with the CU vaccine in the drinking water and suspected of being immunosuppressed.     

Single-Layer Colloid Centrifugation as a Method to Process Urine-Contaminated Stallion Semen After Freezing-Thawing

Urospermia is a major ejaculatory dysfunction affecting stallions. It has been thought that urine-contaminated semen should not be cryopreserved; however, on select cases, urine contamination of semen cannot be avoided. A recent study suggested that urospermic semen can be cryopreserved after cushion centrifugation and extension. Thus, this study aimed to assess the use of single-layer colloid centrifugation (SLC) to process frozen-thawed urine-contaminated stallion semen. Raw ejaculates (n = 55) from eight stallions were split into three groups: no urine, low (20%), or high (50%) urine contamination. Semen was extended 1:1, cushion-centrifuged, and resuspended at 200 million sperm/mL in BotuCrio. Resuspended semen was loaded in 0.5 mL straws and cryopreserved in liquid nitrogen. Samples were thawed (37°C for 30 seconds) and processed by SLC (400 g/30 minutes). Percentages of total motility (TM) and progressive motility (PM) were assessed with computer-assisted semen analyzer. Sperm viability (%VIAB) and yield were assessed with a NucleoCounter before and after gradient centrifugation. Data were analyzed with two-way ANOVA and Tukey’s test. The motility parameters TM before SLC (control: 35 ± 2; low: 33 ± 0.7; high: 22 ± 1.8) after SLC (control: 51 ± 3.6; low: 42 ± 2.2; high: 25 ± 2.8) and PM before SLC (control: 24 ± 1.8; low: 21 ± 1.14; high: 12 ± 1.5) and after SLC (control: 40.3 ± 3.2; low: 31 ± 3.9; high: 14 ± 2) significantly decreased with increasing urine contamination. Urine contamination marginally reduced (P < .05) sperm viability after cryopreservation before SLC (control: 45 ± 0.7; low: 27 ± 0.2; high: 27 ± 0.3) and after SLC (control: 54 ± 0.5; low: 49 ± 0.7; high: 38 ± 0.6). Recovery rates of sperm after centrifugation were not significantly different between groups. In conclusion, urine contamination affects sperm motility parameters in a dose-dependent manner. Post-thaw SLC selected sperm with higher motility and viability in control and low groups but only selected sperm with higher viability in the high group.     

不同生育期和凋萎时间对多花黑麦草饲用和发酵品质的影响

本研究利用多花黑麦草Musashi品种, 研究孕穗期和开花期饲草在不同凋萎时间的饲用品质动态及青贮品质动态, 旨在选择出适宜不同生育期多花黑麦草凋萎青贮的时间。实验设置了对照、凋萎1, 2, 3, 4和5 h共6个处理, 分别测定了原料的饲用品质相关性状和青贮料的发酵品质性状。结果表明, 原料的非结构碳水化合物(NSC)含量随着凋萎时间的延长持续下降, 但可溶性碳水化合物(WSC)则呈不同的变化趋势:孕穗期多花黑麦草在凋萎过程中的WSC呈不同程度的上升, 在凋萎1 h时含量达到最高, 为10.62%, 之后小幅下降, 但始终高于对照(P<0.05);开花期多花黑麦草在凋萎过程中的WSC则呈先上升后下降的趋势, 在凋萎3 h时含量达到最高, 为14.46%, 之后下降。孕穗期多花黑麦草不同凋萎时间的青贮发酵品质性状中, pH在凋萎3 h时最低, 为4.05, 随后小幅上升;氨态氮(NH₃-N)含量随着凋萎时间的延长持续下降;乳酸(LA)含量先升后降, 凋萎3 h时含量最高, 达8.49%。开花期多花黑麦草不同凋萎时间的青贮发酵品质中, pH随着凋萎时间的延长持续上升;NH₃-N含量随着凋萎时间的延长呈先下降后回升的趋势, 凋萎2 h处理的含量最低, 为2.03 mg/g;LA含量持续下降, 但凋萎3 h以后LA含量大幅降低, 仅为4.56%, 凋萎5 h时LA含量则大幅降至1.38%。适宜的凋萎处理时间, 应该是原料中的WSC含量较高, 干物质含量适宜;青贮料具有较低的pH、NH₃-N含量和较高的LA含量。综合孕穗期多花黑麦草在不同凋萎时间的青贮品质变化趋势, 以凋萎3 h为佳, 凋萎3 h时原料的干物质含量升至26.59%。开花期则以凋萎2 h处理最佳, 凋萎2 h时原料的干物质含量升至28.03%。     

The metabolism of labelled progesterone in the laying hen

Eight hours after administering 4–14C‐progesterone to laying hens four radioactive metabolites could be detected in the droppings. Excretion of labelled steroid products was still continuing 192 h after the injection. In blood a relatively high level of labelled progesterone was found 8 to 24 h after application. The last traces disappeared from blood after 72 h. Compared with mammals the hen metabolises progesterone at a slower rate.

Certain tissues were also examined 24 h after treatment. The adrenals showed a high level of labelled progesterone metabolites. The liver contained similar steroids as were found in the droppings. In the ovary, pituitary and skeletal muscle low or trace amounts of radioactive steroids were detected.