Interaction of Glyphosate with Zinc for the Yield,Photosynthesis, Soil Fertility and Nutritional Status of Soybean

Application of glyphosate herbicide in genetically modified (GM) soybean [Glycine max (L.) Merrill] in soils with low zinc (Zn) concentration may interfere in the uptake of this and other nutrients, with negative impact on productivity. Thus, an experiment was conducted in greenhouse conditions on Ustoxix Quatzipsamment soil to investigate the effects of the interaction of glyphosate with Zn for the yield, photosynthesis, soil fertility and nutritional status of soybean. The treatments consisted of two soybean varieties [BRS 133 (conventional—NGM) and its essentially derived transgenic line BRS 245RR (GM) with and without glyphosate application] and five Zn rates (0, 5, 10, 20 and 40 mg kg^?1, source zinc sulfate (ZnSO₄)), with four replicates. Except for the copper (Cu) and iron (Fe) concentrations, the introduction of the herbicide-resistant gene is the predominant factor reducing nutrient uptake, photosynthetic (A) rate, stomatal conductance (Gs), leaf chlorophyll and ureide concentrations. The administration of Zn rates lowered the leaf phosphorus (P) concentration, and there was significant increase in Zn concentration in the soil and in the plant. Except for the 20 mg kg^?1 of Zn rate, the use of the herbicide did not affect the shoot dry weight (SDW) and seed yield, and on average, the maximum seed yield was obtained with Zn concentrations of 26.4 and 18.7 mg kg^?1 extracted by Mehlich 1 and diethylenetriaminepentaacetic acid-triethanolamine (DTPA-TEA), respectively.     

Effect of Glyphosate and Zinc Application on Yield,Soil Fertility,Yield Components,and Nutritional Status of Soybean

Glyphosate is a widely used nonselective herbicide for the control of agricultural weeds. It is being increasingly used in glyphosate resistant genetically modified plants. However, there are few studies on its effects on the nutritional status of soybean, particularly on the uptake of zinc (Zn). Two experiments were conducted under field conditions in a Typic Quartzipsamment and an Orthic Ferralsol to investigate the effect of glyphosate application × Zn interaction on soil fertility, yield components, seed yield (SY), shoot dry weight (SDW) yield, and nutritional status of soybean. The five Zn rates 0, 3, 6, 9, and 12 kg ha^?1 were used in two soybean varieties [BRS 133 (conventional—NGM) and its essentially derived transgenic line BRS 245RR (GM), which was divided into: with (+Gly) and without (–Gly) glyphosate application. Only the P (phosphorus) and Zn available concentrations in the soil were impacted by Zn rates. However, the available P concentration only decreased in the soil planted with GM soybean. Mehlich 1 and diethylenetriaminepenta acetic acid–triethanolamine (DTPA–TEA), 7.3 extractants were effective to determine the available Zn. In the two crop sites, the number of pods per plant (NPP) and the SDW yield were affected by the interaction varieties × Zn. SY was influenced by the application of the herbicide, reducing a potential phytotoxic effect with the use of high rates. Regarding the nutrients, only the foliar calcium (Ca), boron (B), iron (Fe), and manganese (Mn) concentrations were negatively affected by glyphosate, and in the case of Zn, the difference occurred only between the varieties BRS 133 and BRS 245RR.     

Variability on Yield,Nutritional Status,Soil Fertility,and Potassium-Use Efficiency by Soybean Cultivar in Acidic Soil

The use of cultivar with nutrient-use efficiency is an important strategy in the management of plant nutritional status, particularly potassium (K), because its high demand and the progressive impoverishment caused by the use of inadequate amounts cause frequent deficiency symptoms observed in soybean [Glycine max (L.) Merrill] crops. This study was conducted in greenhouse conditions in a completely randomized design with four replicates in an Typic Quartzipsamment soil aimed to assess the effect of applying two rates of K (50 and 200 mg kg^?1) on growth, shoot dry weight yield (SDWY) and seed yield (SY), nutritional status, yield components, and efficiency of K use in eleven cultivars of different characteristics and growth habits. The SDWY, SY, number of seeds per pod, number of pods, and estimated 100-seed weight showed significant interaction between cultivar and the K rates, with greater values at the rate 200 mg K kg^?1. Similarly, the concentration of nitrogen (N), phosphorus (P), K, calcium (Ca), magnesium (Mg), sulfur (S), boron (B), copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn) in leaves and grains varied according to the K rates and in the cultivar. The most K-use efficient cultivars were BMX Magna RR, BRS 232, BRS 284, BRS 294RR, NA 5909RR, and Vmax RR, whereas FTS Campo Mourão RR was inefficient. Regarding response to fertilization, the cultivars Vmax RR, BMX Magna RR, NA 5909RR, BRS 284, and BRS 294RR were found to be efficient and responsive, whereas the cultivar FTS Campo Mourão RR, BRS 232, BMX Potência RR, BRS 295RR, TMG 1066RR, and TMG 1067RR are inefficient and responsive to K application in the soil.     

Dunite in Agriculture: Physiological Changes,Nutritional Status and Soybean Yield

ABSTRACT

Brazil is an importer of fertilizers and the use of alternative sources is increasing in agriculture. The objective of this study was to evaluate the effect of Dunite rates on magnesium (Mg), silicium (Si), reducing sugars, sucrose and foliar starch, soil chemical attributes and soybean yield [Glycine max (L.) Merril] in two soil types. The treatments consisted in the five Dunite rates (0, 42, 208, 542, and 1542 mg kg^?1) in a clayey soil, and five Dunite rates (0, 150, 238, 411, and 933 mg kg^?1) in a sandy soil. In both crops and soils, the Mg and Si contents, reducing sugars and foliar glucose, as well as pH, Mg and Si of the soil, and yield components showed a positive response due to the increase of input rates. The Mg nutrition provides lower foliar starch levels, consequently, the best partition of metabolites to plant leads to better development, filling and yield of soybeans.     

Glyphosate effects on photosynthesis,nutrient accumulation,and nodulation in glyphosate‐resistant soybean

Previous greenhouse studies have demonstrated that photosynthesis in some cultivars of first‐ (GR1) and second‐generation (GR2) glyphosate‐resistant soybean was reduced by glyphosate. The reduction in photosynthesis that resulted from glyphosate might affect nutrient uptake and lead to lower plant biomass production and ultimately reduced grain yield. Therefore, a field study was conducted to determine if glyphosate‐induced damage to soybean (Glycine max L. Merr. cv. Asgrow AG3539) plants observed under controlled greenhouse conditions might occur in the field environment. The present study evaluated photosynthetic rate, nutrient accumulation, nodulation, and biomass production of GR2 soybean receiving different rates of glyphosate (0, 800, 1200, 2400 g a.e. ha^–1) applied at V2, V4, and V6 growth stages. In general, plant damage observed in the field study was similar to that in previous greenhouse studies. Increasing glyphosate rates and applications at later growth stages decreased nutrient accumulation, nodulation, leaf area, and shoot biomass production. Thus, to reduce potential undesirable effects of glyphosate on plant growth, application of the lowest glyphosate rate for weed‐control efficacy at early growth stages (V2 to V4) is suggested as an advantageous practice within current weed control in GR soybean for optimal crop productivity.     

Development and Nutrition of Soybeans with Macronutrients Deficiencies

Soy is to one of the main crops in the world. However, there are aspects related to its management that should be explained, especially regarding its mineral nutrition, because a proper nutritional balance is associated with productivity levels. The objective of this study was to evaluate the accumulation of nutrients in the tissues related to the deficiency of nutrients and its effects on plant development. In addition, nutritional disorder symptoms were evaluated according to the deficiency of macronutrients. For this, seven treatments were evaluated. They comprised a complete nutrient solution followed by deficiency of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S) in a completely randomized design with three replications. Plant height, number of leaves, leaf area, stem diameter, relative chlorophyll content, and production of dry matter of soybean plants were evaluated. The deficiency of any macronutrient affects biometric variables, especially the production of dry matter. The nutrients that limited the most the production of dry matter were Ca>N>K>Mg>P > S. With the exception of S, all other macronutrients, when suppressed, caused nutritional disorder symptoms. The plants presented an accumulation of K > N>Ca>P>Mg>S in leaves. In the present study, soybean plants had a high nutritional requirement of K followed by N. This requires care in the development of fertilization programs in view of the essential roles these nutrients play in the life cycle of plants.

Abbreviations: N_Nitrogen; P_Phosphorus; K_Potassium; Ca_Calcium; Mg_Magnseium; S_Sulfur; DM_Dry matter; NL_number of leaves; H_plant height; SD_stem diameter; RCI_Relative chlorophyll index; AP_Aerial part; R_Roots; EP_Entire plant; DMS_significant mean difference; CV_Coefficient of variation; pH_hydrogen potential; NaOH_sodium hydroxide; HCl_hydrochloric acid.     

Humic Substances on Soybeans Grown Under Water Stress

Hypothetically, humic substances (HSs) can improve the response of plants in sandy soils. The objective was to assess the effect of applying an organomineral fertilizer enriched with HSs on soybean plants grown with and without water stress. The experimental design was entirely randomized, in a greenhouse, in a 2 × 5 factorial setup (two moisture levels and five fertilizer doses: 0, 1, 2, 4, and 8 mL dm^?3), for a total of 10 treatments, with eight repetitions, using as substrate a Psamment. The organomineral fertilizer was applied in the soil 21 days after plant emergence, and the water regimes were established 1 week thereafter. Plant height, shoot and root dry mass, mineral nutrition, and grain yield were evaluated. There was a positive response to the doses, with the most efficient one under water stress being 6.5 mL dm^?3 and HSs favored the uptake of micronutrients.     

Soybean nodulation and nitrogen fixation on soil previously amended with sewage sludge

Summary A greenhouse study was conducted to examine the residual effects of sewage sludge on soybean Glycine max (L.) Merr., nodulation, and N fixation. Nodulating and nonnodulating isolines of Clark soybean were grown to the R2 stage in soils (Typic Paleudults) obtained from plots where heat-treated sludge had been applied in 1976 at rates equal to 0, 56,112, and 224 Mg ha^–1 high (7.0) and low (6.2) soil pH regimes were established by CaCO₃ additions. Sludge and soil pH treatments resulted in clearly defined differences in metal uptake by soybean shoots. Plant Zn, Cd, and Ni concentrations were greater on pH 6.2, sludge-amended soil than on the pH 7.0, amended soil. At low soil pH, soybean Zn and Cd concentrations, respectively, increased from 41 and 0.19 mg kg^–1 (control) to 120 and 0.58 mg kg^–1 at the 224 Mg hat sludge rate. At the high soil pH and 224 hg hat sludge rate, Zn and Cd concentrations were 45 and 0.15 mg kg^–1, respectively.Symbiotic N fixation provided 90% of the total N accumulation. Total N accumulation, shoot N concentration, dry matter, and N fixation by nodulating soybeans exhibited a significant linear increase with sludge rate. Total N accumulation, dry matter, and N fixation were significantly greater at high soil pH. For high and low soil pH, respectively, N fixation increased from 422 and 382 mg N per plant (control) to 614 and 518 mg N per plant at the 224 Mg ha^–1 sludge rate. While soybean nodulation also increased linearly on sludge-amended soil, a significant rate times pH interaction for nodule number indicated that nodulation was less strongly enhanced by sludge at low soil pH.     

Influence of Lime and Gypsum on Yield and Yield Components of Soybean and Changes in Soil Chemical Properties

Soybean is one of the most important legume crops in the world. Two greenhouse experiments were conducted to determine the influence of liming and gypsum application on yield and yield components of soybean and changes in soil chemical properties of an Oxisol. Lime rates used were 0, 0.71, 1.42, 2.14, 2.85, and 4.28 g kg^?1 soil. Gypsum rates applied were 0, 0.28, 0.57, 1.14, 1.71, and 2.28 g kg^?1 soil. Lime as well as gypsum significantly increased grain yield in a quadratic fashion. Maximum grain yield was achieved with the application of 1.57 g lime per kg soil, whereas the gypsum requirement for maximum grain yield was 1.43 g per kg of soil. Lime significantly improved soil pH, exchangeable soil calcium (Ca) and magnesium (Mg) contents, base saturation, and effective cation exchange capacity (ECEC). However, lime application significantly decreased total acidity [hydrogen (H) + aluminum (Al)], zinc (Zn), and iron (Fe) contents of the soil. The decrease in these soil properties was associated with increase in soil pH. Gypsum application significantly increased exchangeable soil Ca, base saturation, and ECEC. However, gypsum did not change pH and total acidity (H + Al) significantly. Adequate soil acidity indices established for maximum grain yield with the application of lime were pH 5.5, Ca 1.8 cmol_c kg^?1, Mg 0.66 cmol_c kg^?1, base saturation 53%, Ca saturation 35%, and Mg saturation 13%. Soybean plants tolerated acidity (H + Al) up to 2.26 cmol_c kg^?1 soil. In the case of gypsum, maximum grain yield was obtained at exchangeable Ca content of 2.12 cmol_c kg^?1, base saturation of 56%, and Ca saturation of 41%.     

Efficiency of Sulfur Application on Soybean in Two Types of Oxisols in Southern Brazil

Modern agricultural techniques have been increasing the yield of soybean (Glycine max (L.) Merr.) while also causing increasing removal of sulfur (S) from the soil. Besides this, the use of concentrated fertilizers with this element and inadequate soil management, with consequent formation of organic matter with low S concentrations, has been causing frequent symptoms of deficiency in the plants. To assess the effect of S on soybean yield and to establish critical levels of sulfur sulfate (S-SO₄^2-) available in the soil, two experiments were conducted over a 2-year period in the Paraná State, Brazil, in fields containing Typic Haplorthox and Typic Eutrorthox soils, located in the Ponta Grossa and Londrina Counties, respectively. The experimental design was randomized blocks with five S rates (0, 25, 50, 75, and 100 kg ha^?1) and four replicates. The source used was elementary S with 98 percent purity. The maximum estimated yields on average for the 2 years were obtained with application of 49.9 and 63.0 kg ha^?1 in the Typic Haplorthox and Typic Eutrorthox soils, for an overall average of 56.4 kg ha^?1, with concentrations of available S-SO₄^2- in the 0- to 20-cm depth of 16.9, 19.3m and 17.1 mg kg^?1, respectively, values greater than the 10 mg kg^?1 indicated as the adequate concentration for soybean plant. In turn, at the 21- to 40-cm depth, the S concentrations were 49.5, 74.2, and 56.4 kg ha^?1. The efficiency of the fertilization diminished with increasing S rates, in both soil types, while the greatest yield efficiency was obtained in the plants grown in the Typic Haplorthox soil.     

Phosphorus,Potassium and Sulfur Interactions in Soybean Plants on a Typic Hapludox

Soybean (Glycine max (L.) Merril) has the largest acreage in Brazil where nutrients are provided to crops by formulated fertilizers, which is the most usual method. Under tropical and subtropical conditions, most of the nutrients required by soybean crops are phosphorus (P) and potassium (K), while sulfur (S) is applied in smaller amounts. The P, K, and S interactions under greenhouse conditions using completely randomized blocks in a factorial 3 × 3 × 3 arrangement were evaluated. The treatments were as follows: three P rates (0, 75, and 150 mg P kg^?1); three K rates (0, 150, and 300 mg K kg^?1); and three S rates (0, 50, and 100 mg S kg^?1). The P, K, and S application had a significant influence on the shoot dry weight (SDW) and number of pods per pot (NPP) in contrast with the increase in grain yield (GY) that had positive relationship with K and S rates. These results indicated that both nutrients were the most limiting to GY. In soil and leaves, only the P, K, and S concentrations were modified by the treatments (P, K, and S), but in the grains there was synergism of P rates with Mg concentration unlike K rates with Ca concentration. Physiological components as photosynthetic rate, intrinsic efficiency of water use, and chlorophyll had influence from P and K rates.     

Soybean response to copper applied to two soils with different levels of organic matter and clay

Abstract

Increasing the amount of soil organic matter (OM) alters the availability of copper (Cu) for plants under tropical and subtropical conditions. With the aim of evaluating the effects of the OM/Cu interaction on the soybean crop, a trial was conducted with a fully randomized 2?×?5 factorial design and four replicates. The treatments consisted of five Cu rates (0, 1, 2, 4 and 8?mg kg^?1) and two soil types: Typic Oxisol and Typic Ultisol. The soybean responded to fertilization with Cu, producing the highest estimated grain yield at a rate of 4.1?mg kg^?1. Similarly were also observed for shoot dry weight, number of pods and root length. The soil chemical properties and nutrient levels in the leaves and grain were influenced only by the soil type, whereas physiological components were affected in terms of photosynthetic rate and intercellular CO₂ concentration.     

Mineral nitrogen impairs the biological nitrogen fixation in soybean of determinate and indeterminate growth types

Soybean (Glycine max Merrill) crop production in Brazil relies mainly on biological nitrogen fixation (BNF) for nitrogen (N) supply. Recent adoption of indeterminate growth-type genotypes has raised doubts on the need for supplemental mineral N that might negatively affect the BNF. We assessed the effects of mineral N on BNF attributes of soybean genotypes grown in central and southern Brazil. Genotypes were inoculated with Bradyrhizobium sp. and/or received mineral N in three sets of experiments. In the first set, two genotypes received increasing rates of mineral N in nutrient solution, which consistently reduced the BNF. In the second set, mineral N applied at sowing and/or topdressing reduced nodulation and ureides-N in determinate and indeterminate growth-type genotypes. In the third set, mineral N applied at R5.3 stage, foliar or as topdressing, did not increase grain yield in four field experiments. Mineral N impaired BNF irrespective of the growth type and had no effect on grain yield.     

Boron and Amino Acid Foliar Application on Wheat-Soybean Intercropping in a Non-Tillage System

Micronutrient and amino acid (AA) foliar fertilization has generally been sprayed onto plants to increase the crop yield. The experiment had the aim of evaluating the foliar boron (B) and AA application on grain yield (GY), physiological characteristics, nutritional status, and yield components in wheat (Triticum aestivum L.) and soybean [Glycine max (L.) Merrill] intercropping in a non-tillage system (NTS). The experiment was set up as a randomized block design with eight treatments and four replicates. The treatments had the following boron (B) rates: [0, 1, 2, 4, and 8 kg ha^?1, source: boric acid (H₃BO₃)] + AAs (2 L ha^?1) applied by foliar spraying and the additional treatments [(Control - without B and AAs), 2 kg ha^?1 B, 2 L ha^?1 AAs and 2 kg ha^?1 B + 1 L ha^?1 AAs] applied at the end of the elongation and spike beginning of wheat plants and development growth stage (V5) of soybean for two growing seasons. Boron and AAs had no influence on the physiological and yield components and had no increases in the foliar and grain B content in wheat and soybean. No matter the dose, the foliar B + AAs (2 L ha^?1) application did not increase the GY in wheat-soybean under a rotational NTS in loamy soil with suitable available B.     

Bioavailability of Nutrients in Seeds from Tropical and Subtropical Soybean Varieties

The selection of varieties or species of plants with higher nutrient uptake efficiency and nutrient concentration for biofortification of food crops is a key tool to reduce malnutrition. Soybean (Glycine max L. Merr) is one of the most important food crops, because it is consumed directly or indirectly, in the form of seeds, processed (milk and/or derivatives), or used as a protein component of animal feed worldwide. In order to select plants with higher nutrients concentration in seeds, 24 soybean varieties for tropical and subtropical conditions and different general features were assessed. There was great variability in photosynthesis rate, chlorophyll content, seed yield (SY), and concentration and uptake of nutrients by seeds between the varieties. Not genetically modified (NGM) crops showed higher nitrogen (N), cooper (Cu), and manganese (Mn) concentration and higher N, potassium (K), Cu, iron (Fe), Mn, and zinc (Zn) uptake, while for genetically modified (GM) crops only calcium (Ca) concentrations were higher. Varieties BRS 284 and BMX Magna RR showed the highest nutrients concentrations in the group with the highest nutrient efficiency. The genetic variability observed among the varieties regarding uptake and translocation of nutrients into seeds allows selecting more promising materials to be used in the biofortification of nutrients in soybean seeds.     

Systemic Effect of a Brassinosteroid on Root Nodule Formation in Soybean as Revealed by the Application of Brassinolide and Brassinazole

Leguminous plants form nitrogen-fixing root nodules and the number of nodules is controlled by a self-regulating mechanism called autoregulation. However, signaling substances involved in nodule regulation have not been identified. In the present study, we used brassinolide, a most effective molecular species of plant hormone brassinosteroids, and brassinazole, an effective inhibitor of brassinosteroid biosynthesis to determine whether brassinolide played a role in systemic regulation of noduel formation in wild type soybean and its super-nodulating mutant. Foliar application or direct injection of brassinolide into the root base inhibited nodule formation and root development in the super-nodulating mutant (En6500), but not in the parent line (cv. Enrei). The internodes in the plants subjected to foliar application were significantly longer than those in the untreated plants. In contrast, the application of brassinazole on mature leaves or into the culture media resulted in the increase of the nodule number in Enrei. These treatments also inhibited internodal growth in Enrei. The results indicate that brassinosteroids may regulate the nodule number in soybean plants. The function of brassinosteroids for the systemic regulation of nodule formation was examined.     

Agronomic Efficiency of Two Types of Lime and Phosphate Fertilizer Sources in Brazilian Cerrado Soils Cultivated with Soybean

With the increase in phosphate fertilizer prices, there is a need to find lower-cost alternatives that are as efficient as soluble sources such as single (SSP) and triple superphosphate (TSP). In Brazil’s northern and northeastern regions, there are reserves of igneous rocks with low solubility containing high concentrations of total phosphorus (P) that can be used to produce fertilizers, such as thermalphosphates. To assess the efficiency of sources of P and two types of lime on soybean yield, a field experiment was carried out in an area with dystrophic Red Latosol (Oxisol) in a Cerrado region in the southern part of Maranhão State. The experimental design was randomized blocks in a 2 × 3 × 4 factorial scheme, with four replicates. The treatments were two types of lime [calcitic (CL) (<5 dag kg^?1 of MgO) and dolomitic (DL) (>13 dag kg^?1 of MgO)], three phosphate fertilizer sources [triple superphosphate (TSP), “Yoorin” thermalphosphate (YT), and experimental thermalphosphate (ET)], and four rates of phosphorus pentoxide (P₂O₅; 0, 100, 200, and 300 kg ha^?1). After 2 years of cultivation, the application of DL resulted in greater soybean yields than the application of CL. The two lime types influenced the pH, carbon (C), calcium (Ca), and magnesium (Mg) concentrations as well as the Ca/Mg, Ca/K, and Mg/K ratios in the soil. With respect to sources of P, the YT applied in the soil with DL produced an agronomic efficiency index (AEI) similar to that of TSP, whereas in the soil with CL, the TSP, YT, and ET were similar, with maximum technical efficiency (MTE) under both conditions starting at 230 kg ha^?1 of P₂O₅. The critical concentration of available phosphorus (P) in the soil (Mehlich-1 extractant) for cultivation of soybean under the climate and soil conditions studied was between 5.0 and 6.0 mg kg^?1.     

Overcoming the Stressful Effect of Low pH on Soybean Root Hair Curling using Lipochitooligosacharides

Abstract

Signal molecules are among the major factors required for the legume–bacteria symbiosis. The excretion of signal molecules by plants stimulates the bacterial Nod genes resulting in the production of lipochitooligosacharides (LCOs). LCOs cause root hair deformation (RHD) and induction of nodule cells division, leading to the formation of nodules. The chemical structure of LCOs determines their biochemical activities; for example, removal of the sulfate group can significantly reduce the morphogenic activities of LCOs. Stressful conditions interrupt the excretion of signal molecules by the legumes' roots and consequently the inhibition of LCO production by the bacteria. This research has studied the effect of different concentrations of LCOs on RHD of two soybean [Glycine max (L.) Merr] cultivars, AC Bravour and Maple Glen, under acidity stress. In the first experiment, two different concentrations of LCO (10^?7 and 10^?6M) and in the second experiment, three different concentrations of LCO (10^?7, 10^?6, and 10^?5M) were added to the soybean roots subjected to the pH levels of 4, 5, 6, and 7 for 24 h. By microscopy observation, the ratios of RHD were determined. Addition of LCOs resulted in RHD in both cultivars. Maple Glen roots responded similarly to different concentrations of LCO, whereas roots of AC Bravour responded differently. The concentration of 10^?5M LCO could inhibit the stressful effect of pH 4 on RHD compared with pH 7 in both cultivars. The significant interaction between LCO and pH suggests that the effect of LCO on RHD may be more significant under higher levels of acidity.     

HMMER及同源比对预测大豆病程相关蛋白

病程相关蛋白（pathogenesis related proteins,PRs）是病理或病理相关环境下诱导产生的一类蛋白,它的产生与积累是植物体应答生物或非生物胁迫的主要特征之一。近年来大量PR蛋白被鉴定,根据它们的结构特征,生物功能以及进化关系等将PR蛋白分为14个家族。然而,在重要的粮食和油料作物的大豆中发现的PR蛋白却很少,本文通过搜索拟南芥、水稻、玉米以及豆科植物所有的已有的PR蛋白,根据其保守结构域利用BLAST程序和HMMER程序同时预测大豆中可能存在的PR蛋白,通过两种方法的预测和比较整合,共得到大豆9个家族的36个PR蛋白序列。并对它们的连锁群分布、基因结构、基因长度及进化关系进行了详细的分析。发现PR家族成簇分布于Gm05、Gm10、Gm13、Gm15、Gm17、Gm19和Gm20等几个连锁群,基因普遍存在序列较短,大部分都小于1000bp,且内含子数目较少,结构相对简单的特点。在PR4家族中,其家族成员亲缘关系都非常相近,而PR1-4和PR1-3等与该家族其它成员亲缘关系较远的情况。本研究结果预测的PR蛋白为大豆抗病育种以及抗病基因工程研究提供了良好的基础,同时为大豆中其它家族基因预测研究以及其它物种基因家族研究提供参考方法。     

Growth and Nutritional Disorders of Eggplant Cultivated in Nutrients Solutions with Suppressed Macronutrients

The objective was to evaluate the effects of omitting macronutrients in the nutrients solution on growth characteristics and nutritional status of eggplants. The treatments were complete nutrients solution and solutions with nutrient omission: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S). The experiment was carried out under greenhouse conditions with three replicates in a completely random design. Plant height, number of leaves per plant, leaf area, relative chlorophyll index, photosynthesis rate, stomatal conductance, dry matter, concentration levels of macronutrients in plant aerial part and root system, and nutritional disorders were evaluated. Omitting elements interfered in the concentration of elements in the various plant tissues and this had as consequences limited vegetative growth, reduced dry matter and led to the development of the typical deficiency symptoms of each element. Although potassium was the most demanded of all elements, nitrogen and calcium were the most growth limiting ones.