The effect of sucrose on the flower bud opening of ‘Madelon’ cut roses

Complete flower bud opening is characterised by an increase in the petal area, and by enhanced dry and fresh weights of the petals. The import of dry matter into the petals is used for osmotic, biosynthetic and respiratory demands. However, flowers of ‘Madelon’ cut roses frequently fail to open completely under postharvest conditions. An aqueous solution containing 45 mM sucrose induces proper flower bud opening, even when the cut roses have been exposed initially to a sucrose-free solution for 48 h. This points to a requirement for a supply of organic matter from source tissue to the flower as main sink. This conclusion is confirmed by the observation that complete flower bud opening can also be achieved without any addition of sucrose by a reduction in the number of participating petals. Replacing two-thirds part of the 45 mM sucrose by an isomolalic amount of KNO₃ does not affect the flower opening process, implying that a considerable amount of the added sucrose is claimed for osmoregulation. The role of added sucrose and the contribution of the endogenous storage pool in the process of flower bud opening is discussed.     

The effect of cutting carnations in the bud stage on quality and yield of flowers from a nursery

The harvesting of carnation buds at early stages of development resulted in an increase of 10–60% (depending upon the growing-period and the cultivar) in the number of newly formed flower buds, even when the carnations were grown during a period of 11 months. A 22-month period of cultivation resulted in a larger increase in the number of buds.Tight buds could be successfully opened with opening-solution to fully developed flowers, without losing their quality when compared with control flowers harvested at the half-bloom stage.     

The effect of sucrose on the expression of the VvTFL1 and VFL genes during flower development in the “Xiangfei” grapevine

VFL and VvTFL1 genes expression patterns and the effects of sucrose on the expression of VFL and VvTFL1 genes in different organs of the “Xiangfei” grapevine (Vitis vinifera L.) were investigated. VvTFL1 gene expression was detected in the meristem of the apical bud and lateral bud, but was not detected during inflorescence differentiation and flower organ development. After sucrose treatment, VvTFL1 gene expression increased in the apical bud, but decreased in the lateral bud. These results suggested that the VvTFL1 gene might be mainly involved in the apical growth process of shoots, and exogenous sucrose had an effect on the VvTFL1 gene by increasing shoot apical meristem initiation of apical buds. The VFL gene was expressed primarily during inflorescence differentiation and early flower organ development, but it gradually reduced in later flower development. After sucrose treatment, VFL gene expression increased in the inflorescence and small or middle flower, but a little change was seen in the large flower. These results suggested that the VFL gene plays important roles in the initiation of inflorescence meristems and the morphological formation of flower organs. Exogenous sucrose had an effect on VFL gene expression at the early stage of flower development.     

Modelling the effects of soil water potential on growth and quality of cut chrysanthemum (Chrysanthemum morifolium)

A complete dynamic model was developed to describe the effects of soil water potential (WP) on the growth and external quality of standard cut chrysanthemum (Chrysanthemum morifolium) in order to optimise water management of greenhouse crops. Experiments using chrysanthemum cv. ‘Jinba’ with different planting dates and levels of water treatment were conducted in a lean-to type greenhouse from 2006 to 2008. The dynamics of leaf area index (LAI), dry matter partitioning, and external quality traits (plant height, number of leaves per plant, flower-head diameter and peduncle length) were first determined as functions of accumulated photothermal index (PTI). Impacts of WP on leaf photosynthetic rate, LAI, dry matter partitioning, and the external quality traits were quantified via introducing the experimentally identified effects of WP on the parameters in the light response curve of leaf photosynthetic rate and the PTI-based functions. These quantitative relationships were incorporated into a generic crop growth model SUCROS. Using independent experimental data, the model was found to give good predictions for biomass production, dry weight of organs, and the external quality traits of the chrysanthemum cultivar grown under different levels of water supply. The coefficient of determination (r²) between the predicted and measured results was 0.91 for LAI, 0.88 for biomass production, and varied between 0.83 and 0.93 for organ dry weight and the external quality traits. Further evaluation is needed when applying this model to a wider range.     

Effects of chemical and organic fertilizers on the growth,flower quality and nutrient uptake of Anthurium andreanum, cultivated for cut flower production

Sustainable agriculture has become a concern, due to the pressures of the “energy crisis” and issues of “environmental protection”. The use of organic fertilizer made from agricultural waste regenerates natural resources and reduces the consumption of fossil energy as well as phosphorus (P) and potassium (K) deposits. There is scant information available concerning the use of organic fertilizer as the sole source of nutrients in flower production, especially in the cultivation of flowers in a soilless condition. The objective of this study was to develop an organic fertilization management system to replace the chemical fertilization management of the cut flower production of Anthurium andreanum Lind. cultivated under soilless conditions. Four fertilization treatments were carried out consisting of two chemical fertilizers [controlled release fertilizer (CRF) and a chemical nutrient solution (CNS)], and two organic fertilizers [pea and rice hull compost (PRHC) and cattle dung with tea leaf residue compost (CDTC)]. The effects of the various fertilizations on A. andreanum were evaluated based on plant growth, nutrient uptake, and cut flower quality during the 1-year experimental period. The results show that the growth, yield, and cut flower quality of plants receiving PRHC were the same as those receiving CNS and CRF, indicating that PRHC can substitute for CRF and CNS as a nutrient source for cut flower production of A. andreanum cultivated in soilless condition. The plants that received the CNS and PRHC treatments had a significantly increased leaf number and new leaf growth area than those that received the CRF and CDTC treatments. The plants receiving the CDTC showed the lowest increase in leaf area and number of flowers. The retardant growth of plants treated with CDTC has been explained as being due to less carbon (C) being assimilated, most likely as the result of an insufficient supply of nitrogen (N) and manganese (Mn) toxicity. The petiole and peduncle length of the plants receiving the CRF were the shortest, which might be due to the low level of potassium (K) accompanying the magnesium (Mg) deficiency. Even though there were significant differences in the N and K concentrations of the plants among the different treatments, no significant differences were observed in the cut flower quality. In short, the organic fertilizer PRHC can meet the nutrient requirements of A. andreanum cultivation for the cut flower production under soilless conditions.     

The effect of plant-associative bacteria (Azospirillum and Pantoea) on the fruit quality of sweet pepper under limited nitrogen supply

This study investigates the influence of a commercial product, Biopron^®, consisting of the bacteria Azospirillum brasilense and Pantoea dispersa on sweet pepper fruits (Capsicum annuum L.) under limited N supply. When the N supply was reduced from 12 (control) to 7 mmol L⁻¹, the concentration of total-N in the fruits was significantly reduced in both inoculated and non-inoculated plants. The N supply or inoculation did not affect the dry matter content or fruit firmness, but non-inoculated fruit with low N showed a decrease in pericarp thickness and a significant increase in the color parameter a^* compared with the control. Under limited N, inoculation increased the concentration of citric, ascorbic and succinic acids in green fruit compared with non-inoculated fruit, which showed lower values than control fruit. At a later (yellow) stage of development, only succinic acid showed a response to inoculation. Fruit peroxidase (EC 1.11.1.7) activity in fruit of inoculated plants was lower than that observed for non-inoculated fruit grown at both high- and low-N. In contrast, in yellow fruit, total phenolic compounds were increased under N limitation, with no inoculation effect. Our study shows that the effect of plant associative bacteria is not directly related with the increased potential availability of nutrients for uptake, especially for fruit quality characteristics.     

Effects of light,macronutrients, and sucrose on germination and development of the endangered fern Adiantum reniforme var. sinense (Adiantaceae)

Effects of light, macronutrients strength of Murashige and Skoog (MS) (1962), and sucrose in the culture medium on spore germination and gametophyte development of the endangered fern Adiantum reniforme var. sinense were investigated. The presence of light was found to be essential for both spore germination and gametophyte growth. Moreover, a medium consisting of 1/4 MS with 15 g/l sucrose was optimal for spore germination and early gametophyte development; whereas, MS medium with 30 g/l sucrose was optimal for further gametophyte development. Increasing amounts of sucrose (45–60 g/l) in the medium delayed gametophyte growth and development. Additionally, sporophyte formation and early growth of gametophytes in a medium consisting of clay and peat (v/v = 1:2) was higher than those in a medium consisting of pure river sand. These findings indicated that requirements for nutrients for spore germination and early gametophyte development of A. reniforme var. sinese were relatively low, but these increased with further gametophyte development, formation and growth of sporophytes.     

Effects of diurnal temperature alternations and light quality on growth,morphogenesis and carbohydrate content of Cucumis sativus L.

The effects of the difference (DIF) between day/night temperature (DT/NT) and end-of-day (EOD) light quality on growth, morphology, dry matter (DM) content and carbohydrate status in cucumber (Cucumis sativus L.) were examined. Plants were grown under a 12 h high light intensity period and DT/NT of 25/19 °C (positive DIF) or 19/25 °C (negative DIF) in combination with an exposure of 30 min EOD-red (EOD-R) or far-red (EOD-FR) light. A significant interaction between DIF and EOD light quality was found on morphology, DM and carbohydrate content in axial plant organs like stems and petioles, but not in leaf blades and roots. Positive DIF induced taller stems, and higher DM and carbohydrate content than negative DIF when the plants were grown under EOD-R. The stems developed under EOD-FR were tall and accumulated the highest content of DM and carbohydrates and only small differences were found between positive DIF and negative DIF. Under EOD-R a higher content of glucose and fructose was found under positive than negative DIF, while EOD-FR light resulted in a high glucose and fructose content under both positive and negative DIF and thereby equalized the effect of the two temperature treatments. The results show that positive DIF can induce similar responses in elongation growth, DM and carbohydrate accumulation as EOD-FR, and further that phytochrome status interact with the responses to alternating DT and NT.     

高温短时热空气处理对枇杷品质和冷害的影响

为了研究高温短时热空气处理对枇杷果实冷害和品质的影响,采用45℃热空气2、3、4 h处理采后枇杷（解放钟）果实,分析冷藏（5℃）期间其品质和相关生理指标的变化。结果表明,经45℃3 h热处理的果实冷藏后品质最佳,冷害最轻。该处理能显著降低果实失重率、硬度和果皮难剥离程度的上升,保持较高的果肉出汁率;提高果实冷藏期间超氧...     

The effect of trunk electric vibration on the growth,yield and fruit quality of peach trees (Prunus persica [L.] Batsch)

To determine the effect of electric vibration on the growth, yield and fruit quality of peach electric vibrators that provided intermittent perturbation of 6500 rpm for 15 min every 6 h were firmly attached to the trunks of peach trees. Electric vibration resulted in the reduction of shoot length by 80% but had no significant effect on fruit weight, acid content and Brix. In another experiment, electric vibrators (for 15 min every 2 h) were attached to the branches after summer pruning. The regenerated shoots from the treated branches showed more than 500% reduction in length compared to the untreated ones. Even the regenerated shoots of untreated branches nearby the vibrator showed 60% reduction in length. Ethylene production and ACC content in the shoot tips of treated branches were greater than that from control ones.     

The effect of nitrogen source and concentration,medium pH and buffering on in vitro shoot growth and rooting in Eucalyptus marginata

This work examined the effect of nitrogen source and medium buffering on the micropropagation of Eucalyptus marginata Donn ex Sm. The number of shoots was increased when media contained 2-(N-morpholino) ethanesulfonic acid (MES) but this increase was minor and only applied to one of the two clones tested. Highest root production was obtained when the medium contained 7.5 mM nitrogen in a ratio of 2NO₃⁻:1NH₄⁺ and was buffered with 10 mM MES. In the rooting medium the pH was influenced most significantly by the nitrogen source, and then whether the medium was buffered. The media pH remained relatively constant when nitrate was the sole nitrogen source and this was assisted by the addition of 10 mM MES. Lower concentrations (<10 mM) of MES were less effective in buffering media over a four-week culture period in both shoot multiplication and rooting medium.     

The effect of high temperature interruptions during inductive period on the extent of flowering and on metabolic responses in olives (Olea europaea L.)

The effect of the duration of high temperature interruption and the timing of its occurrence during inductive period on the extent of inhibition of inflorescence production in ‘Arbequina’ olive trees was investigated. Trees kept under inductive conditions in different growth chambers were subjected to high daytime temperature (26 ± 1 °C) interruptions for 3, 6, and 12 days. There was no significant difference in the extent of flowering between trees given an uninterrupted induction period and the trees where inductive period was interrupted with high daytime temperatures for three days. Inflorescence production was significantly reduced by both 6 and 12 days high temperature interruptions. Number of flowers per inflorescence was significantly reduced only with 12 days high temperature interruption. Since there was no significant difference between the extent of inhibition of inflorescence by 6 and 12 days high temperature interruption, therefore, 6 days high temperature interruption was used in subsequent experiments to study the effect of timing of interruption. A six day interruption of high temperature produced significant reduction (more than 83%) in inflorescence production irrespective of the time of interruption (i.e., 40 or 50 days after the start of induction) or number of interruptions. None of these treatments had any significant effect on the number of flowers per inflorescence. Higher levels of free arginine were found in trees that had greatest number of inflorescences produced under inductive conditions without any high temperature interruption.     

The effect of light and of explant orientation on the regeneration and subsequent growth of bulblets on Lilium longiflorum Thunb,bulb-scale sections cultured in vitro

Lilium longiflorum, cultivar ‘Osnat’, bulb-scale sections were cultured in the light and in the dark with explants placed with their dorsal (abaxial) side on the medium, and in the dark with their ventral (adaxial) side up or down, or in the upright or inverted position. Scale sections regenerated bulblets, roots and callus. In all cases, bulblets developed only on the ventral side of the scale. Light did not affect the regeneration capacity of the scale piece but did significantly affect bulblet growth. Bulblets developing in the light were small and bore many leaves. In the dark, a smaller number of bulblets, which were much larger but bore fewer leaves, developed. In the light, leaf dry weight was double that of bulb dry weight, whereas in the dark this proportion was reversed. In contrast to light, explant orientation greatly affected regeneration percentage. Scale sections planted with their ventral side down regenerated fewer and smaller bulbs, less root, but much more callus than those with their dorsal side down. The upright and inverted positions also reduced the number of bulblets regenerated and increased callus formation.     

The effect of various nitrogen fertilization and foliar nutrition regimes on the concentrations of sugars,carotenoids and phenolic compounds in carrot (Daucus carota L.)

The aim of this research was to determine the influence of various forms, diverse doses, and dates of application of nitrogen fertilizers and foliar nutrition on the concentration of sugars, carotenoids and phenolics compound in carrot. Two field experiments (Experiment I in 2003–2005 and Experiment II in 2004–2005) with carrot ‘Kazan F₁’ were conducted in Trzciana (50°06′N; 21°85′E) in Poland. Both experiments were arranged in a split-plot design with four replications. Two sub-blocks were identified in both experiments: sub-block (A) without foliar nutrition and sub-block (B) with plant foliar nutrition. In sub-block (B), plants were sprayed three-times with: 2% (w/v) urea, a 1% (v/v) solution of multi-component ‘Supervit R’ fertilizer, and again with 2% (w/v) urea. Combinations with diversified nitrogen fertilization were distinguished within both sub-blocks. The treatments in Experiment I consisted of: (1) Control, (2) 70 kg N ha⁻¹ as Ca(NO₃)₂, (3) 70 + 70 kg N ha⁻¹ as Ca(NO₃)₂, (4) 70 kg N ha⁻¹ as (NH₄)₂SO₄ and (5) 70 + 70 kg N ha⁻¹ as (NH₄)₂SO₄, where 70 kg N ha⁻¹ was used preplant and 70 + 70 kg N ha⁻¹ was applied preplant and as a top dressing, respectively. The treatments in Experiment II consisted of: (1) Control, (2) 35 + 35 kg N ha⁻¹ as ENTEC-26, (3) 70 + 70 kg N ha⁻¹ as ENTEC-26, (4) 105 + 105 kg N ha⁻¹ as ENTEC-26, (5) 35 + 35 kg N ha⁻¹ as NH₄NO₃, (6) 70 + 70 kg N ha⁻¹ as NH₄NO₃, (7) 105 + 105 kg N ha⁻¹ as NH₄NO₃, where 35 + 35, 70 + 70, 105 + 105 kg N ha⁻¹ was applied preplant and as top dressing, respectively. Solid nitrogen fertilizer was added to the soil, as produced: Ca(NO₃)₂—Yara International ASA (Hydro), (NH₄)₂SO₄—Zak?ady Azotowe w Tarnowie, Poland, NH₄NO₃—Zak?ady Azotowe w Pu?awach, Poland and ENTEC-26–COMPO GmbH & Co., KG, Germany. In Experiment I, the highest sugar concentrations were found in carrot fertilized with (NH₄)₂SO₄ 70, while in Experiment II in the control and after fertilization with ENTEC-26 35 + 35 kg N ha⁻¹. In both experiments N-fertilization affected an increase in phenolic compound concentrations in comparison with the control. Experiment I revealed no significant effect of N-fertilization on carotenoid concentrations in carrot, however in Experiment II the highest concentration of these compounds was characteristic for the control plants and carrot fertilized with ENTEC-26 35 + 35. The foliar nutrition applied in Experiment I caused a decline in sugar concentration and an elevated carotenoid concentration, however it had no influence on the phenolic compound concentrations in carrot. Yet the foliar nutrition in Experiment II led to a decrease in phenolic and carotenoid compound concentrations, but it did not affect sugar concentration in carrot.     

The effects of low boron on growth,gas exchange,boron concentration and distribution of ‘Newhall’ navel orange (Citrus sinensis Osb.) plants grafted on two rootstocks

The effects of low boron (B) on plant growth, photosynthesis, B concentration and distribution of ‘Newhall’ orange (Citrus sinensis Osb.) plants grafted on either Trifoliate orange (Poncirus trifoliata (L.) Raf.) or Carrizo citrange [C. sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf.] rootstocks were investigated. One-year-old plants of the two scion-rootstock combinations were grown for 183 days in sand:perlite (1:1, v/v) medium under greenhouse conditions. The plants were irrigated with half-strength Hoagland's nutrient solutions containing four B concentrations (0.01, 0.05, 0.10 and 0.25 mg l⁻¹). The growth of root, stem of scion and leaves was less affected by low B treatments when ‘Newhall’ scion was grafted on Carrizo citrange than on Trifoliate orange. Thus, the growth of scions under low B conditions was mainly depended on the rootstock used, i.e., Carrizo citrange-grafted plants were more tolerant to low B compared to the plants grafted on Trifoliate orange. Boron concentrations in all plant parts decreased significantly by decreasing the B supply in the nutrient solution. Leaves were the dominant sites of B accumulation and showed the greatest reduction in B concentration compared to the other plant parts, as B concentration in the nutrient solution decreased. Irrespective of the rootstock, B levels in the upper–younger leaves were substantially higher than in basal-older leaves when plants were exposed to low B concentrations (≤0.05 mg l⁻¹), suggesting that under such conditions B was preferentially translocated to upper–younger leaves to support their growth. Furthermore, B distribution in different plant parts indicated that there was a restriction in translocation of B from root to scion tissues (stems and leaves of scion) under conditions of limited B availability. In addition, low B treatments decreased leaf photosynthetic rate, stomatal conductance and transpiration rate but increased intercellular CO₂ concentration in the leaves of ‘Newhall’ plants, irrespective of the rootstock used.     

The effect of various nitrogen fertilization and foliar nutrition regimes on the concentrations of nitrates,ammonium ions,dry matter and N-total in carrot (Daucus carota L.) roots

Two field experiments (Experiment I in 2003–2005 and Experiment II in 2004–2005) with carrot c.v. ‘Kazan F₁’ were conducted at Trzciana village (50°06′N, 21°85′E). The experiments were arranged in a split-plot design with four replications. Two sub-blocks were identified in both experiments: I, without foliar nutrition; II, receiving plant foliar nutrition. The plants were sprayed three times alternately with: 2% urea solution, 1% solution of multi-component ‘Supervit R’ fertilizer (produced by Intermag, Poland) and again with 2% urea solution. Combinations with diversified nitrogen fertilization were distinguished within both sub-blocks. Experiment I comprised of: (1) Control, (2) Ca(NO₃)₂ 70, (3) Ca(NO₃)₂ 70 + 70, (4) (NH₄)₂SO₄ 70 and (5) (NH₄)₂SO₄ 70 + 70. Experiment II included: (1) Control, (2) ENTEC-26 35 + 35, (3) ENTEC-26 70 + 70, (4) ENTEC 26 105 + 105, (5) NH₄NO₃ 35 + 35, (6) NH₄NO₃ 70 + 70, (7) NH₄NO₃ 105 + 105. Where 70 kg N ha⁻¹ was used before sowing, whereas 35 + 35, 70 + 70 and 105 + 105 kg N ha⁻¹ were applied before sowing and as top dressing. Solid nitrogen fertilizer was added to the soil (produced by): Ca(NO₃)₂, Yara International ASA (Hydro); (NH₄)₂SO₄, Zak?ady Azotowe in Tarnów, Poland; NH₄NO₃, Zak?ady Azotowe in Pu?awy, Poland; and ENTEC-26, COMPO GmbH & Co. KG, Germany. The research aimed at determining the effect of diversified nitrogen fertilization and foliar nutrition on NO₃⁻, NH₄⁺, N-total and dry matter (d.m.) concentrations in carrot, and N uptake by storage roots. In Experiment I, nitrogen fertilization did not affect NO₃⁻ concentration, whereas in Experiment II, the applied N treatment increased NO₃⁻ concentration in carrot in relation to the control, except for the storage roots of plants fertilized with ENTEC-26 35 + 35. Nitrogen fertilization applied in both experiments caused a significant increase in N-total concentration in carrot and N uptake by storage roots in comparison with the control plants. In both experiments, nitrogen fertilization had a different effect on the concentrations of NH₄⁺ and d.m. in carrot. What is more, foliar nutrition treatments in both experiments had a different effect on the concentrations on NO₃⁻, N-total, d.m. in carrot and N uptake by carrot storage roots.     

The effect of chlormequat chloride (CCC) application at the bolting stage on the flowering and seed production of lettuce plants previously treated with water or gibberellic acid (GA3)

Lettuce plants were sprayed with gibberellin (GA₃) or water at the rosette (8-leaf) stage and subsequently with water or CCC (500 or 1500 ppm) at the onset of bolting. GA₃ induced rapid bolting and increased seed yield, but seed stalks were longer and thinner than those that had been sprayed with water at the same stage. Treatment with CCC (500 ppm) at bolting decreased the flower stalk height of plants that had been sprayed with water at the rosette stage, but not that of GA₃ treated plants. CCC (500 ppm) increased the number of inflorescence branches per plant as well as seed yield in the autumn sown crop, but had no effect on the winter sown crop. When a higher concentration of CCC (1500 ppm) was applied at bolting, flower stalk height decreased, irrespective of whether the plants had been previously treated with GA₃ or not, and seed stalk diameter increased. However, 1500 ppm CCC reduced the number of inflorescence branches per plant and mean seed yield. It is concluded therefore that although the application of 500 ppm CCC at the onset of bolting may increase seed yield in the autumn sown crop, this treatment does not improve the seed stalk strength (e.g. increasing diameter or reducing height) of GA₃ treated plants. On the other hand, whereas a higher concentration of CCC (1500 ppm) improves seed stalk characteristics, it reduces seed yield.     

Effects of water deficit on the vegetative response,yield and oil quality of olive trees (Olea europaea L., cv Coratina) grown under intensive cultivation

An experiment was carried out in a young high-density olive grove (556 plants ha⁻¹—Olea europaea L., cv Coratina) located in Southern Italy to evaluate the effect of different soil water availability on the vegetative and productive performances of olive trees also looking into the quality of the resulting oils. Trials were carried out over a 3-year period on trees subjected to irrigation and grown under rainfed conditions. Vegetative tree response, as shoot elongation and trunk diameter, was evaluated. Yield per plant, fruit characteristics, oil quality indices (free fatty acid content, peroxide value, UV adsorption at 232 and 270 nm, total phenols, α-tocopherol content) were determined for both irrigated and non-irrigated treatments in the ‘on’ years 1997 and 1999 (6th and 8th year after planting, respectively).     

The conjunctive use of compost tea and inorganic fertiliser on the growth,yield and terpenoid content of Centella asiatica (L.) urban

The conjunctive use of a compost tea (CT) and an inorganic fertiliser (NPK) on the growth, yield and terpenoid content of Centella asiatica (L.) urban was evaluated. CT and NPK applied at half the recommended concentration resulted in the significant enhancement of vegetative growth, yield and antioxidant content. The synergistic effect of CT₅₀ and NPK₅₀ was highly pronounced on the bioactive components Asiatic acid, madecassoside and asiaticoside. The distribution of the total antioxidants was highest in roots (80%), followed by leaves (66%) and petioles (54%). There was a strong positive correlation (r = 0.990) between fertility and total antioxidant content, suggesting an enhanced synthesis of bioactive components resulting from the treatment. This response by C. asiatica to the integrated experimental treatment suggests a viable option for the commercial cultivation of the herb. Augmenting the vegetative biomass production and bioactive components will increase the availability of this medicinal herb for the treatment of various human ailments.