Effect of 7-year application of a nitrification inhibitor, dicyandiamide (DCD), on soil microbial biomass, protease and deaminase activities, and the abundance of bacteria and archaea in pasture soils

Purpose

The nitrification inhibitor dicyandiamide (DCD) has been shown to be highly effective in reducing nitrate (NO₃ ^?) leaching and nitrous oxide (N₂O) emissions when used to treat grazed pasture soils. However, there have been few studies on the possible effects of long-term DCD use on other soil enzyme activities or the abundance of the general soil microbial communities. The objective of this study was to determine possible effects of long-term DCD use on key soil enzyme activities involved in the nitrogen (N) cycle and the abundance of bacteria and archaea in grazed pasture soils.

Materials and methods

Three field sites used for this study had been treated with DCD for 7 years in field plot experiments. The three pasture soils from three different regions across New Zealand were Pukemutu silt loam in Southland in the southern South Island, Horotiu silt loam in the Waikato in the central North Island and Templeton silt loam in Canterbury in the central South Island. Control and DCD-treated plots were sampled to analyse soil pH, microbial biomass C and N, protease and deaminase activity, and the abundance of bacteria and archaea.

Results and discussion

The three soils varied significantly in the microbial biomass C (858 to 542 μg C g^?1 soil) and biomass N (63 to 28 μg N g^?1), protease (361 to 694 μg tyrosine g^?1 soil h^?1) and deaminase (4.3 to 5.6 μg NH₄ ⁺ g^?1 soil h^?1) activity, and bacteria (bacterial 16S rRNA gene copy number: 1.64?×?10⁹ to 2.77?×?10⁹ g^?1 soil) and archaea (archaeal 16S rRNA gene copy number: 2.67?×?10⁷ to 3.01?×?10⁸ g^?1 soil) abundance. However, 7 years of DCD use did not significantly affect these microbial population abundance and enzymatic activities. Soil pH values were also not significantly affected by the long-term DCD use.

Conclusions

These results support the hypothesis that DCD is a specific enzyme inhibitor for ammonia oxidation and does not affect other non-target microbial and enzyme activities. The DCD nitrification inhibitor technology, therefore, appears to be an effective mitigation technology for nitrate leaching and nitrous oxide emissions in grazed pasture soils with no adverse impacts on the abundance of bacteria and archaea and key enzyme activities.     

Effects of flue gas desulfurization gypsum by-products on microbial biomass and community structure in alkaline–saline soils

Purpose

For an alkaline?Csaline region in Northwest China, we examined the responses of soil microbial communities to flue gas desulfurization gypsum by-products (FGDB), a new ameliorant for alkaline?Csaline soils. In 2009 and 2010, we collected soils from 0?C20?cm and 20?C40?cm depths along an experimental FGDB gradient (0, 0.74, 1.49, 2.25, and 3.00?kg FGDB m^?2).

Materials and methods

As a measure of microbial community composition and biomass, we analyzed phospholipid fatty acids (PLFAs). We used real-time quantitative polymerase chain reaction (qPCR) to measure abundance of bacterial 16?S rRNA copy numbers. Additionally, physicochemical soil parameters were measured by common laboratory methods.

Results and discussion

Microbial community composition differed along the FGDB gradient; however, the microbial parameters did not follow a linear response. We found that, in 2009, total PLFA concentrations, and concentrations of total bacterial and Gram-negative bacterial PLFAs were slightly higher at intermediate FGDB concentrations. In 2010, total PLFA concentrations, and concentrations of total bacterial, Gram-positive bacterial, Gram-negative bacterial, and fungal PLFAs as well as the fungal:bacterial PLFA ratio were highest at 1.49?kg FGDB m^?2 and 3.00?kg FGDB m^?2. PLFA concentrations often differed between 2009 and 2010; however, the patterns varied across the gradient and across microbial groups. For both years, PLFA concentrations were generally higher at 0?C20?cm depth than at 20?C40?cm depth. Similar results were obtained for the 16?S rRNA copy numbers of bacteria at 0?C20?cm depth. FGDB addition resulted in an increase in soil Ca²⁺ and NO ₃ ^? ?CN and a decrease in pH and electrical conductivity (EC). Shifts in PLFA-based microbial community composition and biomass could partly be explained by pH, soil organic carbon, total nitrogen (TN), soil moisture, EC, inorganic nitrogen, C/N, and Ca²⁺. Indirect effects via shifts in abiotic soil properties, therefore, seem to be an important pathway through which FGDB affect soil microbial communities.

Conclusions

Our results demonstrate that addition of FGDB leads to significant changes in soil physicochemical and microbial parameters. As such, addition of FGDB can have large impacts on the functioning of soil ecosystems, such as carbon and nitrogen cycling processes.     

Shift in abundance and structure of soil ammonia-oxidizing bacteria and archaea communities associated with four typical forest vegetations in subtropical region

Purpose

Nitrogen (N) is one of the most important elements that can limit plant growth in forest ecosystems. Ammonia-oxidizing bacteria (AOB) and archaea (AOA) are considered as the key drivers of global N biogeochemical cycling. Soil ammonia-oxidizing microbial communities associated with subtropical vegetation remain poorly characterized. The aim of this study was to determine how AOA and AOB abundance and community structure shift in response to four typical forest vegetations in subtropical region.

Materials and methods

Broad-leaved forest (BF), Chinese fir forest (CF), Pinus massoniana forest (PF), and moso bamboo forest (MB) were widely distributed in the subtropical area of southern China and represented typical vegetation types. Four types of forest stands of more than 30 years grew adjacent to each other on the same soil type, slope, and elevation, were chosen for this experiment. The abundance and community structure of AOA and AOB were characterized by using real-time PCR and denaturing gradient gel electrophoresis (DGGE). The impact of soil properties on communities of AOA and AOB was tested by canonical correspondence analysis (CCA).

Results and discussion

The results indicated that AOB dominated in numbers over AOA in both BF and MB soils, while the AOA/AOB ratio shifted with different forest stands. The highest archaeal and bacterial amoA gene copy numbers were detected in CF and BF soils, respectively. The AOA abundance showed a negative correlation with soil pH and organic C but a positive correlation with NO₃ ^??N concentration. The structures of AOA communities changed with vegetation types, but vegetation types alone would not suffice for shaping AOB community structure among four forest soils. CCA results revealed that NO₃ ^??N concentration and soil pH were the most important environmental gradients on the distribution of AOA community except vegetation type, while NO₃ ^??N concentration, soil pH, and organic C significantly affected the distribution of the AOB communities.

Conclusions

These results revealed the differences in the abundance and structure of AOA and AOB community associated with different tree species, and AOA was more sensitive to vegetation and soil chemical properties than AOB. N bioavailability could be directly linked to AOA and AOB community, and these results are useful for management activities, including forest tree species selection in areas managed to minimize N export to aquatic systems.     

Soil organic carbon in the rocky desert of northern Negev (Israel)

Purpose

So far, the soil organic carbon (SOC) literature is dominated by studies in the humid environments with huge stocks of vulnerable carbon. Limited attention has been given to dryland ecosystems despite being often considered to be highly sensitive to environmental change. Thus, there is insufficient research about the spatial patterns of SOC stocks and the interaction between soil depth, ecohydrology, geomorphic processes, and SOC stocks. This study aimed at identifying the relationship between surface characteristics, vegetation coverage, SOC, and SOC stocks in the arid northern Negev in Israel.

Materials and methods

The study site Sede Boker is ideally suited because of well-researched but variable ecohydrology. For this purpose, we sampled five slope sections with different ecohydrologic characteristics (e.g., soil and vegetation) and calculate SOC stocks. To identify controlling factors of SOC stocks on rocky desert slopes, we compared soil properties, vegetation coverage, SOC concentration, and stocks between the five ecohydrologic units.

Results and discussion

The results show that in Sede Boker, rocky desert slopes represent a significant SOC pool with a mean SOC stock of 0.58?kg?C?m^?2 averaged over the entire study area. The spatial variability of the soil coverage represents a strong control on SOC stocks, which varies between zero in uncovered areas and 1.54?kg?C?m^?2 on average in the soil-covered areas. Aspect-driven changes of solar radiation and thus of water availability are the dominant control of vegetation coverage and SOC stock in the study area.

Conclusions

The data indicate that dryland soils contain a significant amount of SOC. The SOC varies between the ecohydrologic units, which reflect (1) aspect-driven differences, (2) microscale topography, (3) soil formation, and (4) vegetation coverage, which are of greatest importance for estimating SOC stocks in drylands.     

Nitrate reduction coupled with microbial oxidation of sulfide in river sediment

Purpose

Nitrate (NO ₃ ^? ) is often considered to be removed mainly through microbial respiratory denitrification coupled with carbon oxidation. Alternatively, NO ₃ ^? may be reduced by chemolithoautotrophic bacteria using sulfide as an electron donor. The aim of this study was to quantify the NO ₃ ^? reduction process with sulfide oxidation under different NO ₃ ^? input concentrations in river sediment.

Materials and methods

Under NO ₃ ^? input concentrations of 0.2 to 30?mM, flow-through reactors filled with river sediment from the Pearl River, China, were used to measure the processes of potential NO ₃ ^? reduction and sulfate (SO ₄ ^2? ) production. Molecular biology analyses were conducted to study the microbial mechanisms involved.

Results and discussion

Simultaneous NO₃ ^? removal and SO₄ ^2? production were observed with the different NO ₃ ^? concentrations in the sediment samples collected at different depths. Potentially, NO ₃ ^? removal reached 72 to 91?% and SO ₄ ^2? production rates ranged from 0.196 to 0.903?mM?h^?1. The potential NO ₃ ^? removal rates were linearly correlated to the NO ₃ ^? input concentrations. While the SO ₄ ^2? production process became stable, the NO ₃ ^? reduction process was still a first-order reaction within the range of NO ₃ ^? input concentrations. With low NO ₃ ^? input concentrations, the NO ₃ ^? removal was mainly through the pathway of dissimilatory NO ₃ ^? reduction to NH ₄ ⁺ , while with higher NO ₃ ^? concentrations the NO ₃ ^? removal was through the denitrification pathway.

Conclusions

While most of NO ₃ ^? in the sediment was reduced by denitrifying heterotrophs, sulfide-driven NO ₃ ^? reduction accounted for up to 26?% of the total NO ₃ ^? removal under lower NO ₃ ^? concentrations. The vertical distributions of NO ₃ ^? reduction and SO ₄ ^2? production processes were different because of the variable bacterial communities with depth.     

Evolution and phosphorus fractionation in saline Spolic Technosols flooded with eutrophic water

Purpose

The aim of this study was to evaluate the behaviour of P in saline Spolic Technosols flooded with eutrophic water, with and without plant rhizosphere, in order to assess the role of these soils as sinks or sources of this nutrient.

Materials and methods

Samples were taken from basic (pH?~7.8), carbonated and acidic (pH?~6.2), de-carbonated soils of salt marshes polluted by mine wastes. Three treatments were assayed: pots with Sarcocornia fruticosa, pots with Phragmites australis and pots without plants (bare soil). The pots were flooded for 15?weeks with eutrophic water (PO ₄ ^3? ~6.92?mg?L^?1) and pH, Eh and water-soluble organic carbon and PO ₄ ^3? concentrations were monitored in the soil solution. A soil P fractionation was applied before and after the flooding period.

Results and discussion

The PO ₄ ^3? concentration in the soil solution decreased rapidly in both soils, with and without plant, being diminished by 80?C90?% after 3?h of flooding. The Fe/Mn/Al oxides and the Ca/Mg compounds played an important role in soil P retention. In pots with S. fruticosa, the reductive conditions due to flooding induced P release from metal oxides and P retention to Ca/Mg compounds. In turn, P. australis may have favoured the release of P from carbonates, which was transferred to Fe/Mn/Al compounds.

Conclusions

The retention of P by the soil was the main mechanism involved in the removal of PO ₄ ^3? from the eutrophic flooding water but to evaluate the capacity of these systems as long-term P sinks, the combined effect of metals, Ca/Mg compounds and specific plant species should be considered.     

Characters of soil algae during primary succession on copper mine dumps

Purpose

Algae play an important role in degraded areas during the initial stages of soil formation by improving its physico-chemical properties, reducing the erosion of soil, and thus favoring the settlement of vascular plants. This study investigates the characters of soil algal communities on copper tailing dumps and discusses the contribution of soil algae to the primary succession progress of young mine tailings ecosystems.

Materials and methods

Five representative potential successional series (bare land, algae crust, mixed algal–moss crust, moss crust, and vegetated site) on copper tailing dumps and a nearby reference site were selected. The soil algae were identified using growth slide method, dilution plate method, and by direct microscopic observation of the soil suspensions. All experiments were carried in an incubation chamber at a temperature of 25 °C and with a 16 h/8 h light–dark cycle at a light intensity of 3,000 lux.

Results and discussion

A total of 120 algal species were recorded. Cyanophyta (blue-green algae) were the most diverse taxonomic group, followed by Bacillariophyta (diatoms) and Chlorophyta (green algae), although diatoms were absolutely absent in bare sites. Diversity of soil algae was highest in vegetated site, whereas it was lowest in bare sites. Total algal abundance ranged between 0.15?×?10³ cells/g to 46.8?×?10³ cells/g dry soil, with the lowest abundance in the youngest site and the highest abundance in the mixed algal–moss crust site. Correlation analysis showed that the growth of soil algae was inhibited by high Cu, Zn, and Fe concentrations and low nutrient content and that the green algae were more sensitive to nutrient content than blue-green algae.

Conclusions

Our results suggest that blue-green algae were most diverse, followed by diatoms and green algae. Species and abundance of soil algae in the tailings increased with the early succession process because of the decrease in heavy metal content and the improvement of nutrient conditions. The growth of soil algae created conditions for the settlement and growth of higher plants, but the appearance of moss and vascular plants inhibited the growth of soil algae.     

Geographic distance and amorphous iron affect the abundance and distribution of <Emphasis Type="Italic">Geobacteraceae</Emphasis> in paddy soils in China

Purpose

Geobacteraceae are important dissimilatory Fe (III)-reducing microorganisms, influencing the cycling of metals, nutrients as well as the degradation of organic contaminants. However, little is known about their distribution, diversity, and abundance of Geobacteraceae and the effects of environment factors and geographic distance on the distribution and diversity of Geobacteraceae in paddy soils remain unclear. Therefore, the objectives of this study were to investigate the distribution, diversity, and abundance of Geobacteraceae in paddy soils and to determine key factors in shaping the Geobacteraceae distribution, environmental factors, geographic distance, or both and to quantify their contribution to Geobacteraceae variation.

Materials and methods

Illumina sequencing and quantitative real-time PCR using a primer set targeting 16S rRNA genes of bacteria affiliated with the family Geobacteraceae were employed to measure the community composition, diversity, and abundance patterns of 16S rRNA genes of Geobacteraceae in 16 samples collected from north to south of China. MRT, Mantel test, and VPA were used to analyze the relationship between communities of Geobacteraceae and environmental factors and geographic distance.

Results and discussion

Quantitative PCR showed that the abundance of 16S rRNA genes of Geobacteraceae ranged from (1.20?±?0.18)?×?10⁸ to 1.13?×?10⁹?±?2.25?×?10⁸ copies per gram of soil (dry weight) across different types of soils. Illumina sequencing results showed Geobacter was the dominant genus within the family of Geobacteraceae. Multivariate regression tree (MRT) analysis showed that soil amorphous iron contributed more (22.46 %) to the variation of dominant species of Geobacteraceae than other examined soil chemical factors such as pH (14.52 %), ammonium (5.12 %), and dissolved organic carbon (4.74 %). Additionally, more geographically distant sites harbored less similar communities. Variance partitioning analysis (VPA) showed that geographic distance contributed more to the variation of Geobacteraceae than any other factor, although the environmental factors explained more variation when combined. So, we detected the uneven distribution of Geobacteraceae in paddy soils of China and demonstrated that Geobacteraceae community composition was strongly associated with geographic distance and soil chemical factors including aFe, pH, Fe, DOC, C:N, and NO₃ ^?-N. These results greatly expand the knowledge of the distribution of Geobacteraceae in environments, particularly in terrestrial ecosystems.

Conclusions

Our results showed that geographic distance and amorphous iron played important roles in shaping Geobacteraceae community composition and revealed that both geographic distance and soil properties governed Geobacteraceae biogeography in paddy soils. Our findings will be critical in facilitating the prediction of element cycling by incorporating information on functional microbial communities into current biogeochemical models.

     

Pig manure vermicompost (PMVC) can improve phytoremediation of Cd and PAHs co-contaminated soil by Sedum alfredii

Purpose

A major challenge to phytoremediation of co-contaminated soils is developing strategies for efficient and simultaneous removal of multiple pollutants. A pot experiment was conducted to investigate the potential for enhanced phytoextraction of cadmium (Cd) by Sedum alfredii and dissipation of polycyclic aromatic hydrocarbons (PAHs) in co-contaminated soil by application of pig manure vermicompost (PMVC).

Materials and methods

Soil contaminated by Cd (5.53?mg?kg^?1 DW) was spiked with phenanthrene, anthracene, and pyrene together (250?mg?kg^?1 DW for each PAH). A pot experiment was conducted in a greenhouse with four treatments: (1) soil without plants and PMVC (Control), (2) soil planted with S. alfredii (Plant), (3) soil amended with PMVC at 5?% (w/w) (PMVC), and (4) treatment 2?+?3 (Plant?+?PMVC). After 90?days, shoot and root biomass of plants, Cd concentrations in plant and soil, and PAH concentrations in soil were determined. Abundance of PAH degraders in soil, soil bacterial community structure and diversity, and soil enzyme activities and microbial biomass carbon were measured.

Results and discussion

Application of PMVC to co-contaminated soil increased the shoot and root dry biomass of S. alfredii by 2.27- and 3.93-fold, respectively, and simultaneously increased Cd phytoextraction without inhibiting soil microbial population and enzyme activities. The highest dissipation rate of PAHs was observed in Plant?+?PMVC treatment. However, neither S. alfredii nor PMVC enhanced PAH dissipation when applied separately. Abundance of PAH degraders in soil was not significantly related to PAH dissipation rate. Plant?+?PMVC treatment significantly influenced the bacterial community structure. Enhanced PAH dissipation in the Plant?+?PMVC treatment could be due to the improvement of plant root growth, which may result in increased root exudates, and subsequently change bacterial community structure to be favorable for PAH dissipation.

Conclusions

This study demonstrated that remediation of Cd and PAHs co-contaminated soil by S. alfredii can be enhanced by simultaneous application of PMVC. Long-term evaluation of this strategy in co-contaminated field sites is needed.     

Does the influence of earthworms on water infiltration, nitrogen leaching and soil respiration depend on the initial soil bulk density? A mesocosm experiment with the endogeic species Metaphire posthuma

Soil compaction has a negative impact on both earthworm abundance and diversity. Recent studies, however, suggest that earthworm cast properties are not influenced by the initial soil bulk density. With time, earthworms could therefore transform soils with different bulk densities into a soil with the same physical state and thus with a similar ecological functioning. This study aimed to test this hypothesis in two laboratory incubation experiments. First, we measured the influence of soil bulk density (1.1 or 1.4?g?cm^?3) on the production of cast by the endogeic earthworm species Metaphire posthuma. In a second experiment, we investigated the effect of M. posthuma on water infiltration, NH ₄ ⁺ , and NO ₃ ^? leaching and soil respiration at the same two soil bulk densities. Although initially higher, earthworm casting activity in soil at 1.4?g?cm^?3 decreased until it reached the same level of activity as earthworms in soil at 1.1?g?cm^?3. This behavioral plasticity led to a transformation of compacted and loose soils, with their own functioning, to a third and similar state with similar hydraulic conductivity, nitrogen leaching, and soil respiration. The consequences for soil organization and soil functioning are discussed.     

Impact of organic matter addition on pH change of paddy soils

Purpose

The objective of the present study was to explore the effect of initial pH on the decomposition rate of plant residues and the effect of residue type on soil pH change in three different paddy soils.

Materials and methods

Two variable charge paddy soils (Psammaquent soil and Plinthudult soil) and one constant charge paddy soil (Paleudalfs soil) were used to be incubated at 45 % of field capacity for 105 days at 25 °C in the dark after three plant residues (Chinese milk vetch, wheat straw, and rice straw) were separately added at a level of 12 g?kg^?1 soil. Soil pH, CO₂ escaped, DOC, DON, MBC, MBN, NH ₄ ⁺ , and NO ₃ ^? during the incubation period were dynamically determined.

Results and discussion

Addition of the residues increased soil pH by 0.1–0.8 U, and pH reached a maximum in the Psammaquent and Plinthudult soils with low initial pH at day 105 but at day 3 in the Paleudalfs soil with high initial pH. Incorporation of Chinese milk vetch which had higher concentration of alkalinity (excess cations) and nitrogen increased soil pH more as compared with incorporation of rice and wheat straws. Microbial activity was the highest in Chinese milk vetch treatment, which resulted in the highest increase of soil pH as compared with addition of rice and wheat straws. However, nitrification seemed to be inhibited in the variable charge soils of Psammaquent and Plinthudult but not in the constant charge soil of Paleudalfs.

Conclusions

The effectiveness of increasing soil pH after incorporation of the plant materials would be longer in low initial pH soils of Psammaquent and Plinthudult than in high initial pH soil of Paleudalfs. In order to achieve the same degree of pH improvement, higher amounts of plant residues should be applied in constant charge soils than in variable charge soils.     

Shifts of archaeal community structure in soil along an elevation gradient in a reservoir water level fluctuation zone

Purpose

Although archaea play an important role in nutrients cycling, the archaeal community in a reservoir water-level fluctuation zone (WLFZ) remains unclear. An elucidation of archaeal community responding to the environmental variables is essential to understand the nutrients dynamics in WLFZ. This study focused on the response of the archaeal community structure and abundance to the periodic water flooding along an elevation gradient in the WLFZ of the Three Gorges Reservoir.

Materials and methods

Along the elevation gradient (152–175 m) of the study area, soil samples in the beginning and late stages of water flooding were collected to investigate the influence of water flooding on the archaeal community in soil, using quantitative PCR and Illumina high-throughput sequencing approaches.

Results and discussion

An increase of archaeal abundance from 3.8?×?10⁸ to 3.8?×?10⁹ copies (g d.w.s)^?1 on average was observed after water flooding. The archaeal abundance was positively correlated with the contents of ammonium, organic matter, and moisture in soil and with the accumulated flooding time. Higher diversity was observed in dry samples (non-flooded soil samples) rather than wet samples (flooded soil samples). The Thaumarchaeota were predominant in most of the dry samples. Interestingly, high proportions of Candidatus Nitrososphaera were observed in the transition zone, while euryarchaeotal methanogens dominated the wet samples. The proportion of methanogens decreased dramatically in the dry samples at higher elevations, which was associated with the decrease of the moisture content and the probably increase of available oxygen in soil.

Conclusions

Archaeal abundance, diversity, and community composition shifted along an elevation gradient and were influenced by water flooding. The increased archaea abundance after water flooding and elevation related community composition and diversity indicated that water flooding was a key dynamic environmental variable in the WLFZ.

     

Effects of cultivation and abandonment on soil carbon content of subalpine meadows, northwest China

Purpose

Land use changes have a significant impact on soil carbon emission and sequestration worldwide. Accurate evaluation of the effect of land use change (cultivation and abandonment) on soil carbon content of subalpine meadows is required to monitor the soil carbon dynamics of rangeland ecosystems in China.

Materials and methods

Based on collection of soil cores and vegetation, investigations of four types of land use (undisturbed natural meadow, land cultivated for 20?years, land abandoned for 3?years following cultivation, and land abandoned for 10?years following cultivation) were undertaken in the headwater area of the Heihe River in northwest China. Three soil carbon fractions [soil organic carbon (SOC), light fraction organic carbon (LFOC), and microorganism biomass carbon (MBC)] were determined in the laboratory, and the relative abundances of LFOC/SOC and MBC/SOC were calculated.

Results and discussion

Repeated cultivation by ploughing reduced the carbon content of the top soil layer, resulting in more uniform vertical distribution of soil organic matter. Ten years after cessation of cultivation, the organic carbon content within the top 10-cm soil layer (0?C10?cm) had reached 90?% of the content in native meadows, equivalent to a mean annual sequestration rate of 1.73?t?C?ha^?1. The rate of LFOC restoration was faster than that of SOC restoration. The variation in the ratio of MBC to SOC (0.91?C1.07?%) was small.

Conclusions

The activity of cultivation reduced all indicators of soil carbon status, which were not completely restored to the level of natural meadow, even after abandonment of cultivation for 10?years. Nevertheless, abandonment of cultivation is a practical, even if long-term, means of improving carbon sequestration in subalpine meadow of China.     

Changes in methane emission and methanogenic and methanotrophic communities in restored wetland with introduction of <Emphasis Type="Italic">Alnus trabeculosa</Emphasis>

Purpose

The dynamics and uncertainties in wetland methane budgets affected by the introduction of Alnus trabeculosa H. necessitate research on production of methane by methanogenic archaea and consumption by methane-oxidizing microorganisms simultaneously.

Materials and methods

This study investigated methane emission in situ by the closed chamber method, and methanogenic and methanotrophic communities using denatured gradient gel electrophoresis (DGGE) and quantitative PCR based on mcrA (methyl coenzyme M reductase), pmoA (particulate methane monooxygenase) genes in the rhizosphere and non-rhizosphere soils in the indigenous pure Phragmites australis T., and A. trabeculosa–P. australis mixed communities in Chongxi wetland.

Results and discussion

Methane flux rate from the pure P. australis community was 2.4 times larger than that of A. trabeculosa–P. australis mixed community in the rhizosphere and 1.7 times larger in the non-rhizosphere, respectively. The abundance of methanogens was lower in the mixed community soils (3.56?×?10³–6.90?×?10³ copies g^?1 dry soil) compared with the P. australis community (1.47?×?10⁴–1.89?×?10⁴ copies g^?1 dry soil), whereas the methanotrophs showed an opposite trend (2.08?×?10⁶–1.39?×?10⁶ copies g^?1 dry soil for P. australis and 6.20?×?10⁶–1.99?×?10⁶ copies g^?1 dry soil for mixed community soil). A liner relationship between methane emission rates against pmoA/mcrA ratios (R ²?=?0.5818, p?<?0.05, n?=?15) was observed. The community structures of the methane-cycling microorganism based on mcrA and pmoA suggested that acetoclastic methanogens belonging to Methanosarcinaceae and a particular type II methanotroph, Methylocystis, were dominant in these two plant communities.

Conclusions

The introduction of A. trabeculosa would promote the proliferation of methanotrophs, especially the dominant Methylocystis, but not methanogens, ultimately diminishing methane emission in the wetland.

     

Spatial and vertical distribution of <Superscript>137</Superscript>Cs in soils in the erosive area of southeastern Serbia (Pčinja and South Morava River Basins)

Purpose

The area of southeastern Serbia, the P?inja and South Morava River Basins, is under the influence of very strong erosion, and the aim of this study was to investigate the vertical and spatial distribution of the ¹³⁷Cs in the eroded soils of this area.

Materials and methods

Vertical soil profiles were collected with 5-cm increments from the uppermost layer down to 20, 25, 30, 40, and 50 cm of depth, depending on the thickness of the soil layers, i.e., down to the underlying parent rocks. Measurements of ¹³⁷Cs activity concentration were performed by using the HPGe gamma-ray spectrometer ORTEC-AMETEK (34 % relative efficiency and high resolution 1.65 keV at 1.33 MeV for ⁶⁰Co), from its gamma-ray line at 661.2 keV.

Results and discussion

The mean ¹³⁷Cs activity concentration across all 18 soil profiles (for all soil layers) was found to be 20 Bq kg^?1. In the greatest number of soil profiles, the ¹³⁷Cs activity concentration was generally highest in the first soil layer (0–5 cm) and decreased with soil depth, while in a few soil profiles, the peak of either the ¹³⁷Cs activity concentration occurred in the second soil layer (5–10 cm) or the ¹³⁷Cs activity concentration was almost equal throughout the entire soil profile. The mean ¹³⁷Cs activity concentration in the first soil layer (0–5 cm) was found to be 61 Bq kg^?1, and the high coefficient of variation of 92 % pointed out high spatial variability and large range of the ¹³⁷Cs activity concentrations in the study area.

Conclusions

The obtained results indicate that in the greatest number of soil profiles, ¹³⁷Cs is present in the upper layers, with concentration decreasing with depth, as is typical in uncultivated soil. Its spatial distribution was very uneven among the surface soil layers of the investigated sites. One of the main reasons for such pattern of ¹³⁷Cs in the study area may be soil erosion. Additional investigations which would support this hypothesis are required.

     

Simultaneous Removal of H2S, NH3, and Ethyl Mercaptan in Biotrickling Filters Packed with Poplar Wood and Polyurethane Foam: Impact of pH During Startup and Crossed Effects Evaluation

The present work discusses the startup and operation of different biotrickling filters during the simultaneous removal of NH₃, H₂S, and ethyl mercaptan (EM) for odor control, focusing on (a) the impact of pH control in the stability of the nitrification processes during reactor startup and (b) the crossed effects among selected pollutants and their by-products. Two biotrickling filters were packed with poplar wood chips (R1 and R2A), while a third reactor was packed with polyurethane foam (R2B). R2A and R2B presented a pH control system, whereas R1 did not. Loads of 2?C10?g N?CNH₃ m^?3?h^?1, 5?C16?g S?CH₂S m^?3?h^?1, and 1?C6?g EM m^?3?h^?1 were supplied to the bioreactors. The presence of a pH control loop in R2A and R2B proved to be crucial to avoid long startup periods and bioreactors malfunctioning due to biological activity inhibition. In addition, the impact of the presence of different concentrations of a series of N species (NH ₄ ⁺ , NO ₂ ^? , and NO ₃ ^? ) and S species (SO ₄ ^2? and S^2?) on the performance of the two biotrickling filters was studied by increasing their load to the reactors. Sulfide oxidation proved to be the most resilient process, since it was not affected in any of the experiments, while nitrification and EM removal were severely affected. In particular, the latter was affected by SO ₄ ^2? and NO ₂ ^? , while nitrification was significantly affected by NH ₄ ⁺ . The biotrickling filter packed with polyurethane foam was more sensitive to crossed effects than the biotrickling filter packed with poplar wood chips.     

Management practices have a major impact on nitrifier and denitrifier communities in a semiarid grassland ecosystem

Purpose

Nitrification and denitrification, two of the key nitrogen (N) transformation processes in the soil, are carried out by a diverse range of microorganisms and catalyzed by a series of enzymes. Different management practices, such as continuous grazing, mowing, and periodic fencing off from grazing, dramatically influenced grassland ecosystems. This study aimed to examine the effects of management practices on the abundance and community structure of nitrifier and denitrifier communities in grassland ecosystems.

Materials and methods

Soil samples were collected from a semiarid grassland ecosystem in Xilingol region, Inner Mongolia, where long-term management practices including free-grazing, different periods of enclosure from grazing, and different frequencies of mowing were conducted. Real-time quantitative polymerase chain reaction (Q-PCR), denaturing gradient gel electrophoresis (DGGE), sequencing, and phylogenetic analysis were applied to estimate the abundance and composition of amoA, nirS, nirK, and nosZ genes.

Results and discussion

The ammonia-oxidizing archaea (AOA) amoA copies were in the range 5.99?×?10⁸ to 8.60?×?10⁸, while those of ammonia-oxidizing bacteria (AOB) varied from 3.02?×?10⁷ to 4.61?×?10⁷. The abundance of AOA was substantially higher in the light grazing treatment (LG) than in the mowing treatments. The quantity and intensity of DGGE bands of AOA varied with pasture management. In stark contrast, AOB population abundance and community structure remained largely unchanged in all the soils irrespective of the management practices. All these results suggested that ammonia oxidizers were dominated by AOA. The higher gene abundance and greater intensity of DGGE bands of nirS and nosZ under the enclosure treatments would suggest greater stimulated denitrification. The ratio of nosZ/(nirS?+?nirK) was higher in mowing treatments than in the free-grazing and enclosure treatments, possibly leading to more complete denitrification. Correlation analysis indicated that soil moisture and inorganic nitrogen content were the two main soil environmental variables that influence the community structure of nitrifiers and denitrifiers.

Conclusions

In this semiarid neutral to alkaline grassland ecosystem under low temperature conditions, AOA mainly affiliated with Nitrososphaera dominated nitrification. These results clearly demonstrate that grassland management practices can have a major impact on nitrifier and denitrifier communities in this semiarid grassland ecosystem, under low temperature conditions.

     

Pollution assessment and spatial variation of soil heavy metals in Lixia River Region of Eastern China

Purpose

The effect of soil heavy metals on crops and human health is an important research topic in some fields (Agriculture, Ecology et al.). In this paper, the objective is to understand the pollution status and spatial variability of soil heavy metals in this study area. These results can help decision-makers apportion possible soil heavy metal sources and formulate pollution control policies, effective soil remediation, and management strategies.

Materials and methods

A total of 212 topsoil samples (0–20 cm) were collected and analyzed for eight heavy metals (Cd, Hg, As, Cu, Pb, Cr, Zn, and Ni) from agricultural areas of Yingbao County in Lixia River Region of Eastern China, by using four indices (pollution index (PI), Nemerow pollution index (PI_N), index of geo-accumulation (I _geo), E _i /risk index (RI)) and cluster analysis to assess pollution level and ecological risk level of soil heavy metals and combining with geostatistics to analyze the concentration change of heavy metals in soils. GS+ software was used to analyze the spatial variation of soil heavy metals, and the semi-variogram model is the main tool to calculate the spatial variability and provide the input parameters for the spatial interpolation of kriging. Arcgis software was used to draw the spatial distribution of soil heavy metals.

Results and discussion

The result indicated that the eight heavy metals in soils of this area had moderate variations, with CVs ranging from 23.51 to 64.37 %. Single pollution index and Nemerow pollution index showed that about 2.7 and 1.36 % of soil sampling sites were moderately polluted by Cd and Zn, respectively. The pollution level of soil heavy metals decreased in the order of Cd?>?Zn?>?Pb?>?As?>?Cu?>?Cr?>?Ni?>?Hg. The I _geo values of heavy metals in this area decreased in the order of Zn?>?Cd?>?As?>?Pb?>?Cu?>?Cr?>?Hg?>?Ni. According to the E _i index, except Cd that was in the moderate ecological risk status, other heavy metals in soils were in the light ecological risk status, and the level of potential ecological risk (RI) of soil sampling sites of the whole area was light.

Conclusions

The results of four indices and the analysis of spatial variation indicated that the contents of Cd and Zn were contributed mainly by anthropogenic activities and located in the south-east of this study area. However, the contents of Hg, As, Cu, Pb, Cr, and Ni in soils were primarily influenced by soil parent materials.

     

Spatial variation and interaction of runoff generation and erosion within a semi-arid, complex terrain catchment: a hierarchical approach

Purpose

Closed erosion plots have been used extensively to investigate soil loss and its spatial variation within a watershed. However, erosion rates measured on closed plots at various locations within a watershed may not reflect the “real world” conditions due to plot boundary problems. The purpose of this study was to identify runoff and sediment sources in a semi-arid, complex terrain catchment by using the data collected from open plots, nested catchments, and tunnel systems.

Materials and methods

The study catchment, in the Loess Plateau of China, was partitioned into various-level geomorphic units. Runoff and sediment discharges were measured from 55 storm events between 1963 and 1968 on open plots and nested catchments. Storm flows were also monitored in 14 rainfall events from the tunnel systems between 1989 and 1990. This study combined the data collected from the two periods to investigate runoff and sediment sources from the different geomorphic units of the catchment.

Results and discussion

On the four open plots (S1, S2, S3, and S4) of the hill slope, total runoff depths of 128.5 mm (S1), 84.3 mm (S2), 101.92 mm (S3), and 141.73 mm (S4) were recorded from all the events over the first period, which correspondingly produced total sediment yields of 3.056 kg m^?2 (S1), 9.058 kg m^?2 (S1), 42.848 kg m^?2 (S3), and 97.256 kg m^?2 (S4). The number of runoff events also varied due to a non-uniformity in runoff generation among the different geomorphic units of the catchment. Tunnel flows generally had higher mean sediment concentrations than catchment outflows. Three nested catchments located from the headwaters (C1) to the mouth of the catchment (C3) generated total runoff depths of 120.02 mm (C1), 143.92 mm (C2), and 149.43 mm (C3), and correspondingly produced sediments yields of 62.01 kg m^?2 (C1), 144.02 kg m^?2 (C2), and 123.92 kg m^?2 (C3) for the first period.

Conclusions

Significant variations in runoff and erosion existed within the catchment. The spatial variation of runoff generation on the hill slopes resulted from the variation of soil infiltration. Sediment produced from the lower hill slope zone was disproportionally higher than that from the upper hill slope zone. Nevertheless, a significant portion of the sediment eroded on the lower slope zone was caused by runoff generated from the upper slope zone. Tunnel erosion also played a significant role in sediment production.     

Dynamics of soil extractable carbon and nitrogen under different cover crop residues

Purpose

Cover crop residue is generally applied to improve soil quality and crop productivity. Improved understanding of dynamics of soil extractable organic carbon (EOC) and nitrogen (EON) under cover crops is useful for developing effective agronomic management and nitrogen (N) fertilization strategies.

Materials and methods

Dynamics of soil extractable inorganic and organic carbon (C) and N pools were investigated under six cover crop treatments, which included two legume crops (capello woolly pod vetch and field pea), three non-legume crops (wheat, Saia oat and Indian mustard), and a nil-crop control (CK) in southeastern Australia. Cover crops at anthesis were crimp-rolled onto the soil surface in October 2009. Soil and crop residue samples were taken over the periods October?CDecember (2009) and March?CMay (2010), respectively, to examine remaining crop residue biomass, soil NH₄ ⁺?N and NO₃ ^??CN as well as EOC and EON concentrations using extraction methods of 2?M KCl and hot water. Additionally, soil net N mineralization rates were measured for soil samples collected in May 2010.

Results and discussion

The CK treatment had the highest soil inorganic N (NH₄ ⁺?N?+?NO₃ ^??CN) at the sampling time in December 2009 but decreased greatly with sampling time. The cover crop treatments had greater soil EOC and EON concentrations than the CK treatment. However, no significant differences in soil NH₄ ⁺?N, NO₃ ^??CN, EOC, EON, and ratios of EOC to EON were found between the legume and non-legume cover crop treatments across the sampling times, which were supported by the similar results of soil net N mineralization rates among the treatments. Stepwise multiple regression analyses indicated that soil EOC in the hot water extracts was mainly affected by soil total C (R ²?=?0.654, P?<?0.001), while the crop residue biomass determined soil EON in the hot water extracts (R ²?=?0.591, P?<?0.001).

Conclusions

The cover crop treatments had lower loss of soil inorganic N compared with the CK treatment across the sampling times. The legume and non-legume cover crop treatments did not significantly differ in soil EOC and EON pools across the sampling times. In addition, the decomposition of cover crop residues had more influence on soil EON than the decomposition of soil organic matter (SOM), which indicated less N fertilization under cover crop residues. On the other hand, the decomposition of SOM exerted more influence on soil EOC across the sampling times among the treatments, implying different C and N cycling under cover crops.