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1.

Purpose

Humic substances are recalcitrant and might act as persistent binding agents to form macroaggregates. The focus of this study is in investigating the contribution of humic carbon (HC) to soil aggregation in response to various tillage and residue managements.

Materials and methods

Arable soils following 8-year contrasting managements were collected to determine aggregate size distribution and stability and HC fractions including humic acid (HA) and fulvic acid (FA). The contribution of HC to aggregation was divided into three special effects including positive effect (PE), negative effect (NE), and combined effect (CE), and these effects were measured using aggregate fractionation techniques.

Results and discussion

As well as to promote structural stability, HC bounds predominantly with the silt + clay fraction and secondarily with microaggregates to form larger aggregates. The PE increased with increasing aggregate size, whereas the NE followed the opposite pattern. A positive CE was observed for large and small macroaggregates, whereas the CE for microaggregates and the silt + clay fraction was negative. Compared to continuous tillage, reduced- and no-tillage decreased the PE for large and small macroaggregates by 1.58–30.98% at the 0–20 cm depth, and straw returning also slightly decreased the corresponding PE relative to straw removing. By contrast, a significantly higher NE for small macroaggregates at the 0–10 cm depth while 6.33–81.11% decreases in CE for large and small macroaggregates at the 0–10 cm depth as well as for large macroaggregates at the 10–20 cm depth, were observed under reduced- and no-tillage. The extraction of HC significantly reduced the aggregate stability and reduced- and no-tillage effectively limited its decrease magnitude. Small macroaggregates and microaggregates made larger contributions to soil HC accumulation than did other fractions. An averagely increased contribution from large or small macroaggregates was observed under both reduced-/no-tillage and straw returning at the 0–20 cm depth. A significant and positive relationship was found between the mass proportion of macroaggregates and the HC accumulation in 0–20 cm soil. Large macroaggregates had significantly higher HA/FA ratios than small macroaggregates, and reduced- and no-tillage significantly increased these ratios both in large and in small macroaggregates. The CE for large or small macroaggregates was also significantly negatively correlated with their HA/FA ratios.

Conclusions

Overall, the HC accumulation in soil is likely to play a key role in macroaggregation, but conservation tillage might decrease the contribution magnitude of HC to large or small macroaggregation through increasing the corresponding HA/FA ratios.
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2.

Purpose

Arbuscular mycorrhizal (AM) fungi are crucial for ecosystem functioning and can contribute to the formation and maintenance of soil aggregates through the exudation of glomalin by extraradical hyphae. Monitoring fertilization effects on AM fungi may help us to develop sound management strategies. The objectives of this study were to investigate the impacts of long-term fertilization on AM fungal parameters and to find out the key factor that affects the diversity and function of AM fungi.

Materials and methods

A long-term fertilization experiment established in a sandy loam soil at northern China has received continuous fertilization treatments for 21 years, including control; mineral fertilizers of NK, PK, NP, and NPK; organic manure (OM); and half organic manure N plus half mineral fertilizer N (1/2 OMN). Top soil samples (0–15 cm) from three individual plots per treatment were collected for the analysis of chemical properties and fungal parameters. The population size of soil AM fungi was determined by real-time PCR, and the community composition was analyzed using PCR-denature gradient gel electrophoresis (DGGE), cloning, and sequencing techniques. The external mycelium of AM fungi was assessed using the grid-line intersect method, and the glomalin-related soil protein (GRSP) was extracted with citrate solution using bovine serum albumin as a standard.

Results and discussion

Long-term fertilization significantly increased (P?<?0.05) soil organic C content, AM fungal population, species richness (R), Shannon–Wiener index (H), and GRSP content, except for the P-deficiency (NK) fertilization treatment. OM had a significantly greater (P?<?0.05) impact on AM fungal population and GRSP content compared to mineral fertilizers but significantly decreased the length of external mycelium compared to the control (P?<?0.05). Fertilization also changed the community composition of AM fungi, and the P-deficiency treatment again had the slightest influence. In addition, most species recovered from the DGGE profiles belonged to three genera, Glomus, Diversispora, and Archaeospora. Redundancy analysis showed that the population size and species richness of AM fungi and the GRSP content all significantly correlated to soil organic C content (P?<?0.05).

Conclusions

Long-term P-containing fertilization, especially the application of OM, greatly increased the population size, species richness, and species diversity of AM fungi, as well as the contents of GRSP and soil organic C, but tended to decrease the length of external mycelium, while the P-deficiency fertilization had no such effect, suggesting that P was the key factor to maintain soil fertility as well as soil AM fungal diversity in this sandy loam soil.  相似文献   

3.
The effect of different soil management practices on crust strength and thickness, soil water conservation and crop performance was investigated on a ferric lixisol in a semi-arid environment of eastern Kenya.

The study proved that manure and mulching with minimum tillage have a greater effect on the water balance of crusted soils and maize emergence. There was increase in steady infiltration rates, amount of soil water stored in the soil and better drainage. The physical effect of mulch was less important in the rehabilitation of crusted soils in the study site when it was incorporated into the soil. Manure and surface mulch with minimum tillage should therefore be taken into account in land management and water conservation in the semi-arid areas of Kenya. The response of crops to the improved water availability due to manure with minimum and with conventional tillage and surface mulch was very clear. These management practices should be recommended when considering the effectiveness of soil and water management techniques in the study area.  相似文献   


4.
Abstract. Erosion and excessive runoff from a crusting and hard-setting red-brown earth may he ameliorated with suitable management. A field trial, near Cowra, New South Wales, to assess the long-term effect of different tillage systems was used to compare the effect of direct drilling with conventional district cultivation practices under continuous wheat. The soil was sampled in the eighth year for assessment of the soil macropore structure, measurement of bulk density and hydraulic conductivity under tension. Vertical faces were prepared from resin impregnated blocks and the macropore structure described mathematically and visually using digital images and data generated from these images. Infiltration, bulk density and image analysis data all lead to the same conclusions about changes in pore structure. Under direct drilling no crust was evident, and there was greater macroporosity (> 0.175 mm diameter in section). The treatment effects appeared to be significant to about 30 to 35 mm depth at the time of sampling. Greater root and faunal activity were observed under direct drilling.  相似文献   

5.
实施16年保护性耕作下黑土土壤结构功能变化特征   总被引:3,自引:6,他引:3  
通过东北典型黑土区旱作平地连续16 a保护性耕作田间长期定位试验,研究保护性耕作实施后对土壤结构功能的影响,分析土壤结构功能的生育期动态特征及其与土壤有机碳的相关关系,探究作物产量变化的原因.以玉米-大豆轮作黑土农田为研究对象,设置4个耕作处理:秸秆覆盖免耕(No-tillage with straw returnin...  相似文献   

6.
《Soil Use and Management》2018,34(2):286-296
Different tillage regimes can change soil micro‐environmental characteristics, which may influence the distribution and abundance of soil arthropods. In this study, soil arthropods and soil properties under a winter wheat–summer maize cropping system were investigated in different tillage regimes over four seasons at two depths. The tillage treatments included conventional tillage (CT ), tillage once a year (T1), tillage every two years (T2) and no‐tillage (NT ). The results showed that the primary taxa of soil arthropod were Acarina (65.8%), Collembola (28.5%), Diplura (1.4%) and Coleoptera (1%) over the whole sampling period. The total arthropod density was influenced by tillage treatments and season of sampling. Values for CT plots were significantly greater than those for the NT plots in autumn and spring. In contrast, the Shannon–Wiener diversity index (H’) and the evenness index (J) were relatively higher in CT treatment. The Acarina/Collembola (A/C) ratio was significantly smaller in NT treatment for some sampling seasons as mites were more sensitive to tillage practices. The QBS ‐ar index did not show a clear pattern among treatments in this study. Soil arthropods notably showed seasonal variation in depth stratification. Relative to CT , the total soil arthropods in the NT plots tended to concentrate in the upper layer due to soil compaction in the lower layer. The climate conditions and soil physical properties were the main factors affecting the soil arthropod distribution and composition, as the soil chemical and microbial properties did not differ significantly among all tillage treatments.  相似文献   

7.
The study investigated the performance of three model tillage tools (tines). The experimental tillages were made from flat 8 mm plain carbon steel. They were designated T1, T5, and T20, corresponding to tine widths of 1, 5, and 20 cm respectively. Experiments were carried out in a soil bin filled with sandy clay loam soil at average moisture content 11.5% (dry basis) and 600 kPa average cone index. The plastic limit and liquid limit and plasticity index of the soil are 20%, 31% and 11% respectively. Tests were conducted at forward speeds of 0.28, 1.0, and 2.5 m/s. Depths of operation considered were 35, 70, 150, 200 and 250 mm. Draught measurements were made for the different tines and were also calculated using soil mechanics equation. There was reasonable agreement between measured and predicted draught forces. The effects of depth of operation on draught force of the tines were studied and evaluated. It was observed that draught increased at an increasing rate with depth; the relationship was a curvilinear one best fitted by exponential function. The soil disturbance created as a result was also evaluated and reported in this paper. The parameters used to define soil disturbance of a single tine were: ridge-to-ridge distance (RRD), maximum width of soil cut (WFS), maximum width of soil throw (TDW), after furrow depth (df), height of ridge (hr) and rupture distance (f). They all increased as the depth of operation of the tool increased but less proportionately. The critical depth of the tines was also estimated.The results of analysis of variance showed that tool type and operating depth significantly affected draught at 5% level of significance (p < 0.05) and that, there was interaction between the two factors.  相似文献   

8.
Fertilization with nitrogen (N) or phosphorus (P) can improve plant growth in saline soils. This study was undertaken to determine wheat (Triticum aestivum L; cv Krichauff) response to the combined application of N and P fertilizers in the sandy loam under saline conditions. Salinity was induced using sodium (Na+) and calcium (Ca2+) salts to achieve four levels of electrical conductivity in the extract of the saturated soil paste (ECe), 2.2, 6.7, 9.2 and 11.8?dS?m?1, while maintaining a low sodium adsorption ratio (SAR; ≤1). Nitrogen was applied as Ca(NO3)2?·?4H2O at 50 (N50), 100 (N100) and 200 (N200)?mg?N?kg?1 soil. Phosphorus was applied at 0 (P0), 30 (P30) and 60 (P60)?mg?kg?1?soil in the form of KH2PO4. Results showed that increasing soil salinity had no effect on shoot N or P concentrations, but increased shoot Na+ and chlorine ion (Cl?) concentrations and reduced dry weights of shoot and root in all treatments of N and P. At each salinity and P level, increasing application of N reduced dry weight of shoot. At each salinity and N level P fertilization increased dry weights of shoot and root and shoot P concentration. Addition of greater than N50 contributed to the soil salinity limiting plant growth, but increasing P addition up to 60?mg?P?kg?1 soil reduced Cl? absorption and enhanced the plant salt tolerance and thus plant growth. The positive effect of the combined addition of N and P on wheat growth in the saline sandy loam is noticeable, but only to a certain level of soil salinity beyond which salinity effect is dominant.  相似文献   

9.
A field study was carried out to analyze the short-term (2 years) effect of tillage and crop rotation on microbial community structure and enzyme activities of a clay loam soil. The experimental design was a split-plot arrangement of treatments, consisting of two tillage treatments—ridge tillage (RT) and no-tillage (NT)—in combination with two crop rotation treatments—corn (Zea mays L.) monoculture and a 2-year corn-soybean (Glycine max L.) rotation. Phospholipid fatty acid (PLFA) profiles were used to assess soil microbial community structure. No-tillage resulted in significantly higher total PLFAs compared to the RT treatment, which was accompanied by higher activities of protease, β-glucosaminidase, and β-glucosidase. This suggests a close link between soil microbial communities and enzyme activities in response to tillage. The increase of total microbial lipid biomass in the NT soils was due to the increase in both fungal and bacterial PLFAs. Crop rotation had little effect on soil bacterial communities and enzyme activities, but it significantly influenced soil fungal communities, particularly arbuscular mycorrhizal fungi. Soils under monoculture corn had higher fungal biomass than soils under corn-soybean rotation regardless of tillage treatment.  相似文献   

10.
Understanding the underlying mechanisms driving responses of belowground communities to increasing soil fertility will facilitate predictions of ecosystem responses to anthropogenic eutrophication of terrestrial systems. We studied the impact of fertilization of an alpine meadow on arbuscular mycorrhizal (AM) fungi, a group of root-associated microorganisms that are important in maintaining sustainable ecosystems. Species and phylogenetic composition of AM fungal communities in soils were compared across a soil fertility gradient generated by 8 years of combined nitrogen and phosphorus fertilization. Phylogenetic patterns were used to infer the ecological processes structuring the fungal communities. We identified 37 AM fungal virtual taxa, mostly in the genus Glomus. High fertilizer treatments caused a dramatic loss of Glomus species, but a significant increase in genus richness and a shift towards dominance of the lineage of Diversispora. AM fungal communities were phylogenetically clustered in unfertilized soil, random in the low fertilizer treatment and over-dispersed in the high fertilizer treatments, suggesting that the primary ecological process structuring communities shifted from environmental filtering (selection by host plants and fungal niches) to a stochastic process and finally to competitive exclusion across the fertilization gradient. Our findings elucidate the community shifts associated with increased soil fertility, and suggest that high fertilizer inputs may change the dominant ecological processes responsible for the assembly of AM fungal communities towards increased competition as photosynthate from host plants becomes an increasingly limited resource.  相似文献   

11.
12.
Crop rotation adoption in no‐tillage systems (NTS) has been recommended to increase the biological activity and soil aggregation, suppress soil and plant pathogens, and increase the productivity aiming at the sustainability of agricultural areas. In this context, this study aimed to assess the effect of crop rotation on the arbuscular mycorrhizal fungi (AMF) community and soil aggregation in a soil cultivated for nine years under NTS. Treatments consisted of combinations of three summer crop sequences and seven winter crops. Summer crop sequences consisted of corn (Zea mays L.) monoculture, soybean (Glycine max L. Merrill) monoculture, and soybean–corn rotation. Winter crops consisted of corn, sorghum (Sorghum bicolor (L.) Moench), sunflower (Helianthus annuus L.), sunn hemp (Crotalaria juncea L.), pigeon pea (Cajanus cajan (L.) Millsp.), oilseed radish (Raphanus sativus L.), and millet (Pennisetum americanum (L.) Leeke). Soil samples were collected at a depth of 0–0.10 m for analyses of soil chemical, physical, and biological attributes. Spore abundance, total glomalin, and soil aggregate stability index were higher in the soil under corn monoculture. The highest values of aggregate mean weight diameter were observed in the soybean–corn rotation (3.78 mm) and corn monoculture (3.70 mm), both differing from soybean monoculture (3.15 mm), while winter crops showed significant differences only between sorghum (3.96 mm) and pigeon pea (3.25 mm). Two processes were identified in the soil under summer crop sequences. The first process was observed in PC1 (spore abundance, total glomalin, easily extractable glomalin, pH, P, and Mg2+) and was related to AMF; the second process occurred in PC2 (aggregate mean weight diameter, soil aggregate stability index, K+, and organic matter) and was related to soil aggregation. The nine‐year no‐tillage system under the same crop rotation adoption influenced AMF abundance in the soil, especially with corn cultivation in the summer crop sequence, which promoted an increased total external mycelium length and number of spores of AMF. In addition, it favored an increased soil organic matter content, which is directly related to the formation and stability of soil aggregates in these managements.  相似文献   

13.
Four tillage trials have been performed on moderately well-drained loam soil in southeast Norway for 30–37 years (mean 34), comparing reduced tillage (8–10 cm in spring) with autumn ploughing (25 cm). In some years, additional stubble harrowing in autumn (10–12 cm) was compared with harrowing only in spring. Weeds were controlled with herbicides. Straw residues were retained after around 1990 and no fungicides were used. Grain yields are reported for the last nine years, and compared with earlier years. Results are presented for a number of soil properties measured in recent years. Autumn harrowing gave no consistent yield benefit over harrowing only in spring. There was little difference between ploughed and unploughed treatments in mean grain yields over the whole trial period, and the variability between years was similar in both tillage systems. Relative grain yields, calculated as yields obtained without ploughing in percentage of those obtained with ploughing, appeared to be normally distributed around 100%. Responses were often positive in dry years, and negative in wet years. Reduced tillage gave higher P and K concentrations near the soil surface and slightly lower concentrations in deeper layers. There was little change in their levels, relative to earlier findings. Changes in bulk density and total porosity were mostly attributable to changes in the stratification of organic matter. Reduced tillage increased porosity at 4–8 cm depth and decreased it slightly at 24–28 cm, but there was no change in the intermediate layer. The moisture-holding capacity of the soil was altered little by reduced tillage, and soil aeration properties were satisfactory at all three depths measured. There was no change in the total amount of organic matter stored within the topsoil, despite marked changes in its distribution. Reduced tillage gave significant increases in aggregate stability and an indication of greater earthworm activity.  相似文献   

14.
《Soil & Tillage Research》1987,10(4):347-361
Four different soil-structure types could reproducibly be recognized by soil surveyors working on Dutch sandy loam soils with different management practices. The 4 structure types were soils with a primary ploughpan, a loosened ploughpan, a secondary ploughpan and a grassland structure. These types had significantly different soil-physical properties and rooting depths. Soil-water regimes for a 30-year period were stimulated to obtain quantitative information on the influence of soil structure, as an expression of different soil-management practices, on land qualities such as moisture deficit, aeration status and workability.These land qualities were not significantly different for the 3 structure types of arable land when considering the entire soil profile. However, differences in moisture content and aeration status were significant when undisturbed and disturbed ploughpans were compared. Deep ploughing to disrupt the primary ploughpan should therefore not be encouraged in these soil as long as common field operations remain unchanged. The same land qualities for grassland indicated what may be attained by soil-structure regeneration. The probability of having a workable day in spring or autumn is increased by an estimated 20%, and the number of days with adequate aeration by 10%, in soil-structure types of arable land (A, B and C) as compared with grassland soil structure (D). Moisture deficits in the growing season should be about 50% less.In this study, simulation was not used to simulate soil-structure formation as a function of soil management, but soil structure was used as an input for the models, reflecting different management practices. To that end, the various structure types were characterized by physical measurements.  相似文献   

15.
The present study of arbuscular mycorrhizal (AM) fungi is focused on the identification of AM ecotypes associated with different plants species (Poa annua, Medicago polymorpha, and Malva sylvestris) growing in three contaminated soils with different organic matter, phosphorus, and trace element (TE; Cu, Cd, Mn, and Zn) contents. Soils were amended with biosolid and alperujo compost. Shifts in AM fungal community structure, diversity, richness, root colonization, and plant TE uptake were evaluated. Soil properties and plant species had a significant effect on AM fungal community composition as well as on root colonization. However, AM fungal diversity and richness were only affected by soil properties and especially by soil organic matter that was a major driver of AM fungal community. As soil quality increased, Glomeraceae decreased in favor of Claroideoglomeraceae in the community, AM fungal diversity and richness increased, and root colonization decreased. No effect due to amendment (exogenous organic matter) addition was found either in AM fungal parameters measured or TE plant uptake. Our results revealed that the role of TE contamination was secondary for the fungal community behavior, being the native organic matter content the most significant factor.  相似文献   

16.
17.
Soil degradation is accelerated when perennial crops are converted to annual row crops, primarily due to increased soil disturbance from tillage. Subsequent heavy rainfall may induce soil settling, reduce macroporosity and increase hardsetting upon drying. An experiment involving plow and no-tillage and two simulated rainfall treatments (‘wet’ and ‘dry’) was conducted on Kingsbury clay loam soil in northern New York in 1992 and 1993 to study their effects on soil structure under maize (Zea mays L.) after conversion from alfalfa (Medicago sativa L.), and to evaluate the use of spectral analysis of micropenetrometer observations for studying soil aggregation. Undisturbed soil cores were collected from the row and trafficked and non-trafficked interrow positions at the 0.05 and 0.15 m depths and used for laboratory measurement of soil strength and pore system properties. These well-structured soils show a high contribution (up to 0.15 m3 m−3) of macropores to the total porosity of the soil. Soil strength was generally slightly higher for no-till (NT) than plow till (PT), although only significant in 1992. Soil strength in the surface layer did not change significantly with drying. Spectral density patterns did not show strong treatment effects, although distinct peaks reflect 3.0–3.5 mm stable structural units within macroaggregates. Simulated rainfall treatments and tillage treatments generally did not strongly affect measured soil properties, presumably due to stable soil structure. Structurally stable clay loam soils show little effect of tillage or settling on soil physical properties in the first years after alfalfa to maize conversion, and have good potential for long-term annual crop production if properly managed.  相似文献   

18.
A 3-year field study was conducted to evaluate the effect of three tillage practices (conventional, zero and reduced/strip) with two nitrogen levels (120 and 150 kg N ha−1) applied in primary strips and three crop residue management practices (removal, burning and incorporation) in secondary strips in wheat after rice. Reduced tillage resulted in significantly higher overall mean wheat yield (5.10 Mg ha−1) compared to conventional (4.60 Mg ha−1) and zero tillage (4.75 Mg ha−1). Residue incorporation resulted in highest mean yield (5.86 Mg ha−1) during third year. Maximum mean yield (6.1 Mg ha−1) was obtained in reduced tillage followed by conventional tillage (5.8 Mg ha−1) under residue incorporation in third year. The weed dry weight recorded at 30 days after sowing was highest (0.3 Mg ha−1) under zero tillage and lowest under conventional tillage (0.16 Mg ha−1). Among crop residue management practices, the highest dry weight of weeds (0.22 Mg ha−1) was recorded under residue incorporation. The highest infiltration rate (1.50 cm h−1) was recorded in residue incorporation followed by residue burning (1.44 cm h−1) whereas; the lowest (0.75 cm h−1) in zero tillage. Soil bulk density was the highest (1.69 Mg m−3) under zero tillage and the lowest in residue incorporation (1.59 Mg m−3). There were no changes in soil available P and K after each crop sequence in relation to tillage practices during first 2 years. Higher organic carbon (5.1–5.4 g kg−1) was measured under zero tillage compared to other treatments. Residue incorporation increased soil organic carbon and available P while higher available K was monitored in burning treatment during the third year. These results suggest that reduced tillage and in situ incorporation of crop residues at 5 Mg ha−1 along with 150 kg N ha−1 were optimum to achieve higher yield of wheat after rice in sandy loam soils of Indo-Gangetic plains of India.  相似文献   

19.
《Applied soil ecology》2009,41(3):518-528
In the southeastern United States, conservation tillage techniques are used to conserve soil nutrients and structure, providing habitat and substrate for biota, which are largely responsible for the mineralization of nutrients in the soil. A deterrent for growers considering the transition to conservation tillage is the delay in soil response (e.g. increased soil carbon, efficient nutrient cycling, impacts on yield) associated with the equilibration of the soil food web. The objective of this study was to determine if the microbial community composition and biomass changed with transition to conservation tillage. Soils sampled from five sites, representing a chronosequence of conservation tillage (from conventionally tilled to 30 year no-till), were collected for fatty acid analysis. Microbial communities were significantly different among sites. Fungi, characterized by 18:2ω6, 18:1ω9, and 18:3ω6c fatty acids, were typically lowest in the conventionally tilled soil, probably due to repeated disruption of the fungal hyphae associated with tillage. In all soils, soil nutrient concentrations, moisture and microarthropod abundance were correlated with microbial structure. Plots in conservation tillage were significantly different from the conventionally tilled plots, but did not exhibit a clear linear pattern across the chronosequence. This evidence that belowground food webs can respond quickly to a cessation in tillage suggests that the delay in soil response may be due more to the time required to build organic matter than to a slow response by the biota.  相似文献   

20.
Field experiments were conducted on a sandy loam soil (Aeric Tropaquent) during 1981 adn 1982 to assess the effects of compaction, puddling and no-till systems on soil physical properties and on rice growth and yield with and without supplementary irrigation. Soil compaction decreased macro- and micro-pores more than puddling or no-till treatments. The equilibrium infiltration rates were 0.12, 0.15 and 1.65 μm s−1 in compacted, ploughed and no-till treatments, respectively. The saturated hydraulic conductivity, void ratio and moisture content at −0.01 and −1.5 MPa water potential followed a similar trend. The mean weight diameters were 2.40, 2.36 and 2.09 mm for compacted, puddled and no-till treatments, respectively. The mean grain yields for 4 consecutive crops were 6.4, 5.1 and 4.9 Mg ha−1, the compacted being significantly greater than the puddled and no-till treatments. Compared with both puddling and no-till treatments, soil compaction resulted in significant yield increases of about 20% under the rain-fed regime and from 34 to 40% in the flooded moisture regime. There was about 26% increase in rice grain yield by continuous flooding over the rain-fed treatments, with the rice producing greater dry matter and biological yields due to a higher uptake of P, Ca, Mg, K, Na, Mn, Fe and Zn.  相似文献   

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