蕨麻多糖对小鼠血清中三种细胞因子水平的影响

通过给小鼠腹腔注射100mg／kg环磷酰胺，建立免疫抑制模型，观察了100、200、400mg／kg剂量蕨麻多糖配合应用后对小鼠血清中细胞因子IL-6、IFN-γ和TNF-α水平的影响。结果表明，蕨麻多糖能明显升高正常小鼠血清中的IL-6水平，呈剂量依赖关系；能升高正常小鼠血清中的IFN-γ水平，而对TNF-α水平无明显影响。环磷酰胺能降低小鼠血清中IL-6、IFN-γ和TNF-α水平，蕨麻多糖能拮抗环磷酰胺的免疫抑制，升高小鼠血清中IL-6、IFN-γ和TNF-α水平，提高机体的免疫功能。     

太子参多糖对环磷酰胺所致肠道黏膜损伤小鼠SIgA、IL-2、IL-6含量的影响

试验将36只18~22 g雄性昆明小鼠随机分为空白对照组、太子参多糖对照组(400 mg/kg体重)、环磷酰胺(CY)模型组、太子参多糖低、中、高剂量组(100、200、400 mg/kg体重),上述各组分别灌喂蒸馏水和多糖,连续灌胃19 d,第20天,除空白对照组和太子参多糖对照组小鼠腹腔注射生理盐水外,其余4组均腹腔注射CY(100 mg/kg体重),24 h处死小鼠,取十二指肠和回肠,放免法测定分泌型免疫球蛋白A(SIgA)、白细胞介素-2(IL-2)、白细胞介素-6(IL-6)的含量,研究太子参多糖对CY所致肠道黏膜损伤小鼠中SIgA、IL-2、IL-6分泌的影响。结果显示,与CY模型组相比,太子参多糖高、中剂量组十二指肠SIgA含量显著升高(P<0.05),太子参多糖高剂量组回肠SIgA含量极显著升高(P<0.01);太子参多糖高剂量组中十二指肠和回肠IL-2含量显著升高(P<0.05),十二指肠IL-6含量极显著升高(P<0.01),回肠IL-6含量显著升高(P<0.05)。上述结果表明太子参多糖在一定程度上能颉颃CY所致的肠道黏膜免疫损伤。     

阴道蝇蛆病对阿拉善双峰驼外周血清中细胞因子IL-4、IL-10、IFN-γ含量的影响

试验选取6峰12岁健康双峰母驼,试验组与对照组各3峰。周期14d,分为一期(感染前,1~3d)、二期(发病期间,4~12d)、三期(蝇蛆落地后,12~14d),每天采集血液获得血清保存,于第4天对试验组进行人工感染蝇蛆。结果显示:对照组一期与试验组一期3种细胞因子含量均无显著性差异(P>0.05);对照组二期与试验组二期IL-10、IFN-γ含量差异显著(P<0.05),试验组含量较高,IL-4含量差异不显著(P>0.05);在三期中,试验组IL-4含量较低,IFN-γ含量较高且与对照组比较均差异显著(P<0.05)。对照组一、二、三期之间3种细胞因子含量差异均不显著(P>0.05);试验组IL-4含量一、二、三期间无显著性差异(P>0.05),IL-10二期含量高于一期且差异显著(P<0.05),IFN-γ二、三期含量均高于一期且差异显著(P<0.05)。结果表明:阴道蝇蛆病对双峰驼外周血清中IL-4含量基本没影响,但会使IL-10、IFN-γ含量升高,在蝇蛆落地后IL-10含量会降低,IFN-γ仍维持在较高水平。     

白细胞介素-10对口蹄疫病毒感染小鼠T细胞增殖及其表达TNF-α、IFN-γ和IL-2的影响

白细胞介素-10(IL-10)增高是口蹄疫病毒(FMDV)感染过程中显著特征之一。本研究旨在探讨IL-10对FMDV感染小鼠外周血T细胞增殖及其表达效应功能相关细胞因子的影响。采用CCK-8和流式细胞术分别检测小鼠外周血T细胞增殖和T细胞表达效应功能相关细胞因子(TNF-α、IFN-γ和IL-2)。结果显示,与对照小鼠相比,FMDV感染小鼠(感染12、24、36和48 h)外周血T细胞对刀豆蛋白A刺激的增殖均显著下降(P<0.05或P<0.01);FMDV感染小鼠的外周血CD4⁺T细胞表达TNF-α和IL-2均显著下降(均P<0.01),CD8⁺T细胞表达TNF-α、IFN-γ和IL-2也显著下降(P<0.01或P<0.000 1)。体内阻断IL-10/IL-10R信号或者敲除IL-10均能显著恢复FMDV感染小鼠外周血T细胞的增殖(P<0.05或P<0.01),但不影响CD4⁺和CD8⁺T细胞表达TNF-α、IFN-γ和IL-2。本研究首次揭示FMDV能抑...     

枸杞多糖和茯苓多糖对免疫抑制小鼠免疫增强及对肠道黏膜的免疫调节作用

研究比较枸杞多糖和茯苓多糖对免疫抑制小鼠的系统免疫功能的影响及2种多糖对肠道黏膜免疫系统的调节作用,通过腹腔注射环磷酰胺建立小鼠免疫抑制模型,观察小鼠脾脏器官指数变化、腹腔巨噬细胞吞噬功能、流式细胞术检测小鼠脾脏T细胞亚群CD4+/CD8+比例及肠派氏结CD3+、CD19+细胞比例、ELISA检测血清及小肠组织中IL-12、IL-4、IFN-γ细胞因子水平变化评价2种多糖对免疫抑制小鼠免疫功能及肠道黏膜的影响。结果显示,2种多糖可提高免疫抑制小鼠脾脏指数,增强腹腔巨噬细胞吞噬功能,影响免疫抑制小鼠CD4+/CD8+细胞亚群比例及肠派氏结T、B淋巴细胞比例和细胞因子水平。结果表明,枸杞和茯苓多糖均对免疫抑制小鼠具有免疫增强作用,茯苓多糖的免疫调节效应显著优于枸杞多糖,且2种多糖均对肠道黏膜免疫系统具有调节作用。     

饲喂枸杞多糖对架子牛免疫球蛋白水平和细胞因子分泌的影响

探究了枸杞多糖免疫增效剂对架子牛抗体水平和细胞因子分泌的影响。选择体重为350 kg左右的西门塔尔架子牛27头,在基础日粮中添加500 g的自制肉牛枸杞多糖免疫增效剂,连续饲喂7 d,分别在开始饲喂的0 d、7 d、14 d采集血样,进行免疫球蛋白、细胞因子及血常规检测。结果表明:饲喂7 d后,IgA水平与0 d和14 d达到显著差异(P0.05),14 d的含量极显著高于0 d (P0.01);IgM 14 d的含量极显著高于0 d和7 d (P0.01);IgG含量,饲喂7 d后、14 d时的含量极显著的高于0 d (P0.01);IL-2、IL-13、IL-17、IL-19含量,饲喂7 d后与0d差异不显著(P0.05),14 d的含量极显著高于0 d和7 d的含量(P0.01);IL-19含量饲喂7 d后显著高于0 d (P0.05),14 d的含量极显著高于0 d (P0.01);IFN-γ和TNF-β总体呈现增长趋势,14 d后含量极显著高于0 d,7 d (P0.01)。白细胞在饲喂7 d后,第14天的含量极显著高于0 d (P0.01);淋巴细胞在饲喂7 d后,与0 d、14 d的差异不显著(PO.05),14 d的含量显著高于0 d (PO.05)。说明,饲喂枸杞多糖免疫增效剂7 d,可提高肉牛免疫球蛋白水平,促进细胞因子的分泌,激活体内淋巴细胞和白细胞,提高肉牛机体免疫力。     

白毛藤多糖对正常小鼠部分细胞因子mRNA表达的调节

目的:探讨白毛藤多糖对小鼠脾脏细胞因子IL-2、IL-12、TNF-α mRNA表达水平的影响。方法:将BALB/c小鼠随机分成4组:白毛藤多糖高(H组)、中(M组)、低(L组)3个剂量组和生理盐水对照组(C组),连续ip 7 d后,用RT-PCR的方法检测脾脏IL-2、IL-12、TNF-α mRNA表达水平。结果:白毛藤多糖M组、H组可明显促进小鼠脾细胞中细胞因子IL-2、IL-12的mRNA表达,IL-2与C组相比具有显著性差异(P<0.05),且IL-12呈极显著差异(P<0.01)。白毛藤多糖L组、M组2个剂量组能增加TNF-α的mRNA表达,与C组相比差异极显著(P<0.01)。结论:白毛藤多糖能有效提高IL-2、IL-12、TNF-α mRNA三种细胞因子的表达。     

枸杞多糖对雏鸡淋巴细胞体外增殖及分泌IL-2的影响

为探讨枸杞多糖(LBP)对雏鸡T、B淋巴细胞体外增殖的影响,将不同浓度的LBP分别加入到用植物血凝素(PHA)刺激的体外培养的雏鸡胸腺、外周血、脾脏淋巴细胞中,以及用细菌脂多糖(LPS)刺激的体外培养的雏鸡法氏囊、外周血、脾脏淋巴细胞中,分别用MTT比色法和ELISA法测定淋巴细胞增殖的变化以及IL-2的分泌量.结果显示:(1)LBP在一定浓度范围内时,均能刺激T、B淋巴细胞的增殖和促进IL-2的分泌,且随着多糖浓度的增加而增加;(2)LBP对同一器官来源的T、B淋巴细胞的OD值随着雏鸡日龄的增长均呈现增长趋势;(3)同浓度LBP对相同日龄雏鸡胸腺T淋巴细胞和法氏囊B淋巴细胞的OD值均高于外周血和脾脏的T、B淋巴细胞OD值.结果表明LBP能够促进雏鸡淋巴细胞的增殖和IL-2的分泌,并且对淋巴细胞增殖的促进作用有一定的量效、时效关系和组织相关性.     

喹胺醇对小鼠血清中细胞因子水平的影响

目的：观察新兽药喹胺醇对小鼠血清中细胞因子TNF-α、IL-2和IL-6水平的影响.方法：采用放射免疫法检测血清中TNF-α、IL-2和IL-6的含量.结果：中剂量组雄鼠血清中TNF-α含量显著高于对照组和其它药物剂量组（P＜0.05）,药后7 d雌鼠低剂量组TNF-α含量显著高于对照组和其它药物组（P＜0.05）;药后7 d雄鼠中剂量组血清中IL-6含量极显著高于对照组（P＜0.01）,14 d雄鼠中、高剂量组IL-6含量极显著高于对照组（P＜0.01）,药后7 d雌鼠中、高、最高剂量组IL-6含量显著低于对照组（P＜0.05）,14 d低剂量组极显著高于对照组（P＜0.01）;雄鼠血清中IL-2含量在7 d和14 d各组间均无显著差异.结论：喹胺醇对小鼠血清中细咆因子TNF-α、IL-2和IL-6水平的影响可能存在性别差异和剂量差异.     

黄芪多糖对雏番鸭生长性能及血清IL-2的影响

选用1日龄雏番鸭60羽,随机分成2组：试验组和对照组,每组30羽,研究黄芪多糖（astragalus polysaccharide,APS）对雏番鸭生长性能和血清白细胞介素-2（interleukin-2,IL-2）水平的影响。黄芪多糖按600mg/L和0mg/L添加到各组雏鸡的饮水中,试验期为14d。结果表明,试验组...     

Effects of dietary supplementation of Lycium barbarum polysaccharides on growth performance,immune status,antioxidant capacity and selected microbial populations of weaned piglets

Lycium barbarum polysaccharides (LBPs) are a complex mixture of highly branched and partially characterised polysaccharides and proteoglycans extracted from the goji berry. This mixture has great potential as a novel feed supplement for pigs. Two trials were conducted to evaluate the effects of supplementation with LBPs on the growth performance, immune status, antioxidant capacity and selected intestinal microbial populations in weaned piglets. In trial 1, a total of 400 weaned piglets [(Yorkshire × Landrace) × Duroc] with an average body weight (BW) of 6.34 ± 0.16 kg (21 days of age) were divided into five groups and fed a basal diet (control group) or a basal diet containing 1,000, 2,000, 4,000 or 6,000 mg/kg LBPs (supplemented at the expense of corn). Supplementation with 4,000 or 6,000 mg/kg LBPs for 2 weeks significantly increased the average daily gain (ADG) and average daily feed intake (ADFI) of the pigs compared with the control group (p < .05). In trial 2, thirty-two 21-days-old weaned piglets (BW: 6.33 ± 0.11 kg) were allotted to a control group (fed with a basal diet) or an experimental group (basal diet containing 4,000 mg/kg LBPs). The experiment lasted for 14 days. Pigs fed LBP diets exhibited an increased ADG and ADFI, and a decreased diarrhoeal incidence compared with those fed the basal diets (p < .05). Supplementation with LBPs increased the serum IgG and IgM levels (p < .05). Dietary LBPs effectively promoted antioxidant defence properties through enhancing the activities of serum, liver superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), in addition to decreasing the malondialdehyde (MDA) content (p < .05). The addition of LBPs increased the amounts of Bacteroidetes in the ileum and caecum and the caecal contents of Lactobacillus spp. and Bifidobacterium spp. (p < .05), while decreased the populations of Escherichia coli and Firmicutes in the ileum and caecum (p < .05) compared with the control group. Our results suggest that dietary supplementation with LBPs can enhance growth performance, immune status and antioxidant capacity, and improve the intestinal microbial populations of weaned piglets.     

Production of biologically active equine interleukin 12 through expression of p35, p40 and single chain IL-12 in mammalian and baculovirus expression systems.

Interleukin-12 (IL-12) is a key cytokine in the development of cell-mediated immune responses. Bioactive IL-12 is a heterodimeric cytokine composed of disulphide linked p35 and p40 subunits. The aim of this study was to verify biologically activity of the products expressed from equine interleukin-12 (IL-12) p35 and p40 cDNAs and to establish whether equine IL-12 could be expressed as a p35/p40 fusion polypeptide, as has been reported for IL-12a of several mammalian species. We report production of equine IL-12 through expression of p35 and p40 subunits in mammalian and insect cells and of a p35:p40 fusion polypeptide in mammalian cells. Conditioned medium recovered from cultures transiently transfected with constructs encoding equine p35 and p40 subunits or single chain IL-12 enhanced IFN-gamma production in cells derived from equine lymph nodes. Preincubation of IFN-gamma inducing preparations with anti-p40 monoclonal antibody resulted in a significant decrease in IFN-gamma induction capacity. Medium recovered from p35 and p40-expressing baculovirus infected cultures enhanced target cell IFN-gamma production and proliferation. Experimental studies in mice and other animals have revealed a therapeutic benefit of IL-12 in cancer, inflammatory and infectious disease and an adjuvant effect in prophylactic regimes. Production of a bioactive species-specific IL-12 is a first step towards an investigation of its potential application in equine species.     

饲喂枸杞枝条颗粒饲料对肉牛增重和一些免疫指标的影响

为探究饲喂枸杞枝条对育肥肉牛增重和相关免疫因子的影响,选择西门塔尔杂交牛24头,随机分为4组,在基础日粮中分别添加0.65 kg/头(低浓度组)、0.975 kg/头(中浓度组)、1.3 kg/头(高浓度组)的枸杞枝条颗粒,不添加为对照组,连续饲喂60 d,在饲喂前和60 d后称重,60 d采集试验牛血样,用定量检测卡...     

枸杞多糖对獭兔营养物质表观消化率、屠宰性能及肉品质的影响

为研究枸杞多糖(LBP)对獭兔营养物质表观消化率、屠宰性能及肉品质的影响,试验选取150只42日龄的獭兔随机分为5组,每组30只獭兔.对照组饲喂基础日粮,LBP组在基础日粮中依次添加剂量为0.1％、0.2％、0.4％、0.6％的LBP,预试期5 d,正式试验期30 d.结果表明:(1)与对照组相比,日粮中添加0.2％、...     

长期单作对枸杞园不同土层土壤微生物代谢活性和多样性的影响

土壤微生物群落多样性及其活性对维持农田土壤生态系统稳定和功能至关重要。前期研究表明,长期单作可导致枸杞园土壤微生物多样性降低和群落结构变异,但对其如何影响土壤微生物代谢活性尚不明确。为研究微生物代谢活性对长期单作的响应,利用Biolog Eco平板分析了枸杞园表层（0~20 cm）和亚表层（20~40 cm）土壤微生物底物利用谱（CLPP）的年代变化规律。结合土壤酶活性和理化性质测定和分析,探索了土壤微生物底物利用谱变化的驱动因子。结果表明,长期单作促进了枸杞园表层土壤微生物群落的代谢活性（P<0.05）,但不影响亚表层土壤微生物的代谢活性和Shannon指数。随种植年限增加,表层土壤微生物对吐温80和衣康酸的代谢活性增加,而亚表层土壤微生物对D-葡萄氨酸和苯乙基胺的利用显著降低（P<0.05）。对不同碳源类型的分析发现,长期单作抑制了枸杞园表层和亚表层土壤微生物对多聚物的利用能力（P<0.05）,说明土壤微生物对复杂有机物的降解能力可能受到干扰。变差分解分析表明,土壤理化性质和真菌丰度是驱动枸杞园土壤微生物代谢多样性变异的主要因子。这些结果表明长期单作干扰了枸杞园,尤其是亚表层土壤微生物的代谢活性,影响了其对复杂有机物的分解过程。在长期单作下,枸杞园亚表层土壤的植物-土壤负反馈作用可能更加严重,应受到更多关注。     

Effect of Pseudostellaria Polysaccharides on SIgA,IL-2 and IL-6 Contents of Intestinal Mucosal Injured Mice Caused by Cyclophosphamide

Effects of Pseudostellaria polysaccharides on the contents of intestinal mucosal SIgA, IL-2 and IL-6 in mice were reported in this paper.36 Kunming male mice with 18 to 22 g were randomly divided into six groups, blank control group, Pseudostellaria polysaccharides control group, cyclophosphamide (CY) model group, and Pseudostellaria polysaccharides low, middle, high dose groups.Each mouse in Pseudostellaria polysaccharides control group and Pseudostellaria polysaccharides low, middle, high dose groups was respectively treated with polysaccharides at the dose of 400, 100, 200 and 400 mg/kg body weight by intragastrical treatment for 19 continuous days, CY was given to each mouse in CY model group and Pseudostellaria polysaccharides low, middle, high dose groups by intraperitoneal injection with 100 mg/kg body weight in the 20th day, duodenum and ileum samples were collected from mice after 24 h, and SIgA, IL-2 and IL-6 contents were determined.The results showed that SIgA contents in duodenum were significantly increased (P<0.05) in Pseudostellaria polysaccharides middle and high dose groups compared to CY model group, SIgA contents in ileum were extremely significantly increased (P<0.01) in Pseudostellaria polysaccharides high dose group compared to CY model group.IL-2 contents in duodenum and ileum were significantly increased (P<0.05), IL-6 contents in duodenum were extremely significantly increased (P<0.01), IL-6 contents in ileum were significantly increased (P<0.05) in Pseudostellaria polysaccharides high dose group compared to CY model group.It was concluded that Pseudostellaria polysaccharide could antagonize intestinal mucosal injury caused by CY.     

PRRSV和PCV2共感染对猪肺泡巨噬细胞细胞因子IL-10、IL-12p40和IFN-γ mRNA转录的影响

用猪繁殖与呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV2)共感染40日龄健康仔猪,利用实时荧光定量PCR技术对共感染仔猪肺泡巨噬细胞(PAM)细胞因子IL-10、IL-12p40和IFN-γ的mRNA转录水平的变化进行了定量分析。结果表明,IL-12p40 mRNA转录从攻毒后3 d开始显著上调(P<0.05),7 d达高峰,14 d开始下降,但仍显著高于对照组(P<0.05),28 d和42 d低于对照组;IL-10 mRNA在3 d显著上调(P<0.05),14 d达到转录高峰(P<0.01),之后逐渐下降,42 d接近对照组水平;IFN-γmRNA转录水平尽管在3 d和28 d时出现2次转录低峰1、4 d和42 d时出现2次高峰,但只在3 d和7 d差异显著(P<0.05)。由此表明,PRRSV和PCV2共感染可导致PAM细胞因子IL-12p40和IL-10 mRNA的转录在感染早期被激活,而IFN-γ mRNA转录则呈较复杂的动态变化,提示PRRSV和PCV2共感染猪PAM的免疫应答、免疫调节和抗感染功能均受到不同程度的影响。     

黄芩白术与PXF对LPS诱导流产小鼠的保胎作用及子宫内IL-10含量的变化研究

胚胎在发育过程中会表达父体抗原。这种带有一半同种异体抗原的胎儿并不被母体免疫系统所排斥。这不仅是由于胎盘屏障的保护作用，更重要的是母体胎儿界面产生的免疫调节因子有保护胎儿的作用[1]。尽管如此，妊娠并不象人们所期望的那样一帆风顺。哺乳动物已着床的胚胎发育到正常分娩，约有40％～60％会发生胚胎丢失，其中2／3(约67％)发生在妊娠早期[2]。生殖免疫学观点认为，这些胚胎丢失或流产与妊娠母体Th1细胞因子反应增强有关[3]。Th1细胞因子反应以细胞免疫功能增强，产生肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)、γ-干扰素(IFN-γ)等细胞因子为特征，