共查询到12条相似文献,搜索用时 15 毫秒
1.
Tan Van Mai Daisuke Fujita Masaya Matsumura Atsushi Yoshimura Hideshi Yasui 《Breeding Science》2015,65(5):420-429
The rice cultivar ASD7 (Oryza sativa L. ssp. indica) is resistant to the brown planthopper (BPH; Nilaparvata lugens Stål) and the green leafhopper (Nephotettix virescens Distant). Here, we analyzed multiple genetic resistance to BPH and the green rice leafhopper (GRH; Nephotettix cincticeps Uhler). Using two independent F2 populations derived from a cross between ASD7 and Taichung 65 (Oryza sativa ssp. japonica), we detected two QTLs (qBPH6 and qBPH12) for resistance to BPH and one QTL (qGRH5) for resistance to GRH. Linkage analysis in BC2F3 populations revealed that qBPH12 controlled resistance to BPH and co-segregated with SSR markers RM28466 and RM7376 in plants homozygous for the ASD7 allele at qBPH6. Plants homozygous for the ASD7 alleles at both QTLs showed a much faster antibiosis response to BPH than plants homozygous at only one of these QTLs. It revealed that epistatic interaction between qBPH6 and qBPH12 is the basis of resistance to BPH in ASD7. In addition, qGRH5 controlled resistance to GRH and co-segregated with SSR markers RM6082 and RM3381. qGRH5 is identical to GRH1. Thus, we clarified the genetic basis of multiple resistance of ASD7 to BPH and GRH. 相似文献
2.
Y. Xue S. Q. Zhang Q. H. Yao R. H. Peng A. S. Xiong X. Li W. M. Zhu Y. Y. Zhu D. S. Zha 《Euphytica》2008,164(3):739-744
In the present study, quantitative trait loci (QTLs) controlling seed storability based on relative germination rate (%) were
dissected using a saturated linkage map and a recombinant inbred lines (RILs) derived from a cross of japonica cultivar Asominori (Oryza sativa L.) and indica cultivar IR24 (Oryza sativa L.). A total of three QTLs (qRGR-1, qRGR-3 and qRGR-9) were detected on chromosomes 1, 3 and 9 with LOD score ranging from 3.45 to 6.95 and the phenotypic variance explained from
16.72% to 28.63%. The IR24 alleles were all associated with seed storability at all the three QTLs. The existence of these
QTLs was confirmed using IR24 chromosome segment substitution lines (CSSLs) in Asominori genetic background (AIS). By QTL
comparative analysis, the QTL, qRGR-9 on chromosomes 9 appeared to be consistent with another rice population, this region may provide an important region for
isolating this responsible gene. These results also provide the possibilities of enhancing Seed storability in rice breeding
program by marker-assisted selection (MAS) and pyramiding QTLs.
Y. Xue and S. Q. Zhang—joint first authors. 相似文献
3.
Indica-japonica hybridization is an important approach for developing superior performing hybrids in rice (Oryza sativa L.). In view of the scanty information available on cooking quality characters in indica-japonica crosses, an investigation was undertaken to estimate genetic and genotype × environment variance and covariance components
of amylose content, gel consistency and alkali digestion value, and to determine the relative importance of direct genetic
effects, maternal genetic effects and cytoplasmic effects in the genetic variations of the three quality characters. Two indica photo-sensitive genic male sterile (PGMS) lines and four japonica varieties were used as parents to make crosses. Genetic model with genotype × environment interactions for triploid endosperm
was used for genetic studies of the three cooking quality characters. Variance component analysis revealed that genetic variations
of the three characters were mainly attributable to direct additive and maternal additive effects, and the three traits had
significant direct and maternal heritabilities. Genotype × environment interactions were mainly dominance × environment (including
direct dominance × environment and maternal dominance × environment) and cytoplasm × environment interactions. Environment
factors could only affect the expression extent of dominant genes, without changing their directions. Predicted values of
genetic effects indicated that the parental lines, ‘VI-70’ and ‘H9304-1’, appeared to be best for amylose content, ‘T 1950’
and ‘Suxuan’ appeared to be best for gel consistency and alkali digestion value.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
4.
Koukham Vilayheuang Ryoko Machida-Hirano Chay Bounphanousay Kazuo N. Watanabe 《Breeding Science》2016,66(2):204-212
Rice (Oryza sativa L.) is the main food for people in Laos, where it has been grown and eaten since prehistory. Diverse landraces are grown in Laos. ‘Khao Kai Noi’, a landrace favored for its eating quality, is held in the nationwide collection of traditional landraces in the Lao national genebank. Genetic diversity is crucial for sustainable use of genetic resources and conservation. To investigate the genetic diversity of ‘Khao Kai Noi’ for conservation, we genotyped 70 accessions by using 23 polymorphic simple sequence repeat markers. The markers generated 2 to 17 alleles (132 in total), with an average of 5.7 per locus. The total expected heterozygosity over all ‘Khao Kai Noi’ accessions was 0.271. Genetic variation was largest among accessions and smallest within accessions. Khao Kai Noi accessions were classified into three different genetic backgrounds, but there was unclear association between the three inferred population and name subgroups and geographical distribution. Most of the accessions were clustered with temperate japonica and showed genetic relatedness to rice from neighboring provinces of Vietnam, suggesting a Vietnamese origin. The results of this study will contribute to the conservation, core collection and future breeding of the Khao Kai Noi population. 相似文献
5.
Sabhavat Bhaskar Naik Dhanasekar Divya Nihar Sahu Raman Meenakshi Sundaram Preetinder Singh Sarao Kuldeep Singh Vattikuti Jhansi Lakshmi Jagadish Sanmallappa Bentur 《Euphytica》2018,214(3):53
The rice brown planthopper (BPH) Nilaparvata lugens (Stål) is one of the major pests of rice across Asia. Host-plant resistance is the most ecologically acceptable means to manage this pest. A rice breeding line RP2068-18-3-5 (RP2068) derived from the land race Velluthacheera is reported to be resistant to BPH populations across India. We identified a new R gene [Bph33(t)] in this line using advanced generation RILs derived from TN1 × RP2068 cross through phenotyping at two locations and linkage analysis with 99 polymorphic SSR markers. QTL analysis through IciMapping identified at least two major QTL on chromosome 1 influencing seedling damage score in seed box screening, honey dew excretion by adults and nymphal survival. Since no BPH R gene has been reported on chromosome 1, we designate this locus as a new gene Bph33(t) which accounted for over 20% of phenotypic variance. Scanning the region for candidate gene suggested two likely candidates a leucine rich repeat (LRR) gene and a heat shock protein (HSP) coding gene. Expression profiling of the two genes in the two contrasting parents and RILs showed induction of the HSP gene (LOC_Os01g42190.1) at 6 h after infestation while LRR gene did not show such induction. It is likely that the HSP represented Bph33(t). 相似文献
6.
Sindynara Ferreira Luiz Antonio Augusto Gomes Wilson Roberto Maluf Isabela Volpi Furtini Vicente Paulo Campos 《Euphytica》2012,186(3):867-873
The use of resistant cultivars is one of the best methods for nematode control and reduction of economic losses caused by these pathogens. Studies of inheritance of nematode resistance in common bean (Phaseolus vulgaris L.) are nonetheless scarce. The present paper reports on the estimation of genetic parameters associated with resistance to the root nematode Meloidogyne incognita race 1 in common beans. Two contrasting bean lines, ‘Aporé’ (P1 = nematode resistant) e ‘Macarr?o Rasteiro Conquista’ (P2 = susceptible), and the generations F1 (P1 × P2), F2 (P1 × P2), BC1(P1) = (F1 × P1) and BC1(P2) = (F1 × P2), were assessed 45 days after nematode inoculation, through a scale related to the number of eggs per gram of root tissue. Dominant genetic effects were inferior in magnitude to additive effects, indicating incomplete dominance of nematode resistance. Dominance was in the direction of increased nematode resistance (i.e., lower number of eggs per g root). Resistance to Meloidogyne incognita race 1 in common bean is under control of a single gene locus, with incomplete dominance of the resistance allele present in ‘Aporé’, but modifier genes affecting its expression appear to be present in the susceptible parent ‘Macarr?o Rasteiro Conquista’. 相似文献
7.
8.
L. Bazrkar A. J. Ali N. A. Babaeian A. A. Ebadi M. Allahgholipour K. Kazemitabar G. Nematzadeh 《Euphytica》2008,164(3):669-677
Tagging of restorer genes for wild abortive (WA) CMS source by studying a 222 individual plants from a F2 population of a cross between IR58025A × IR42686R. The restorer line IR42686R that was used in this study had been previously
derived through random mating composite population (RMCP) involving 12 parents facilitated by IR36 genetic male sterility.
Four Rf genes were tagged to simple sequence repeats (SSR) markers on chromosomes 1, 7, 10, 12 by recessive class analysis. The recombination
frequency between a positive marker and Rf locus was calculated using maximum likelihood estimator assuming that all the 46 extremely sterile individual plants were
homozygous at the targeted Rf locus. The recombination frequency between the marker and the restorer trait were converted to genetic distances using Kosambi
function. A new Rf locus designated as Rf7 on chromosome 12 was found to be linked to RM7003 at a genetic distance of 13.3 cM (LOD 6.12). We report here first, a new
molecular marker (RM 6344) linked to Rf4 locus on chromosome 7 that was previously mapped by trisomic analysis. RM443 and RM315 were flanking the Rf3 gene at a genetic distance of 4.4 (LOD 10.29) and 20.7 cM (LOD 3.98) on chromosome 1, respectively. The Rf6 was flanked on both side with SSR markers RM258 and RM591 at a genetic distance of 4.4 (LOD 10.29) and 23.3 cM (LOD 3.39)
located on chromosome 10. The random mating composite population is an excellent breeding approach to develop superior restorer
lines and for pyramiding different Rf genes of different CMS systems. Rf genes tagged with closely linked SSR markers would be facilitating marker assisted selection
(MAS) in hybrid rice breeding program by reducing time and workload for identifying potential restorers.
L. Bazrkar and A. J. Ali equally contributed to this work. 相似文献
9.
Three studies were initially conducted using five mint cultivars with known disease reactions to verticillium wilt to determine
if inoculation method, root-dip as compared to root-injection, had an affect on the plant growth and disease reaction. The
planting media did not affect the development of wilt symptoms in susceptible varieties, however, the soil/peat based medium
resulted in higher shoot fresh weight at harvest than the perlite/vermiculite medium. Black Mitcham had the highest susceptible
disease rating and greatest reduction in fresh shoot weight from inoculation with Verticillium dahliae at two inoculum concentrations,
104 or 106 microconidia/ml. Native spearmint was resistant and Mentha crispa was moderately resistant, whereas, Murray Mitcham
peppermint and Scotch spearmint were moderately susceptible to verticillium wilt with a corresponding lower fresh shoot weight.
Scotch spearmint was less susceptible at the low inoculum concentration than at a higher level of microconidia, indicating
a low level of disease resistance. Overall, the two inoculation methods resulted in similar cultivar responses to verticillium
wilt, although the root-injection method was more applicable for large plant populations. Thus, the root-injection method
of inoculation was utilized to screen 743 Black Mitcham derived somaclones for wilt resistance, of which nine somaclones were
found resistant in repeated inoculation tests.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
10.
Sijun Zheng Betty Henken Willem Wietsma Eri Sofiari Evert Jacobsen Frans A. Krens Chris Kik 《Euphytica》2000,114(1):77-85
The beet armyworm (Spodoptera exigua Hübner)is the most important pest in tropical Alliumcultivations. All shallot (Allium cepa L. group Aggregatum) cultivars are susceptible to this pest. Therefore accessions from three wild Alliumspecies, namely A. galanthum Kar. et Kir., A. fistulosum L. and A. royleiStearn, next to A. cepa L. were used to screen for resistance. First of all, a reliable bio-assay had to be developed. To this end transparent plastic
cages with in total 5 plants of one accession per cage were placed on per lite in a heated greenhouse. Five 3-day old larvae
were inoculated on each plant. Eight days after inoculation the number of surviving larvae per cage and the mean fresh weightper
larva was determined. The lowest larval survival (36%) was found on A. roylei. This was not, however, significantly different from other Allium accessions. Significant differences were found in the fresh weight per larva fed on different Allium accessions. The larvae survived on A. roylei had a very low fresh weight (10.3 mg per larva), while those on an accession of A. fistulosum had the highest fresh weight (45.1 mg per larva). The larval fresh weight on A. roylei was lower than all the other accessions except from the tropical shallot cultivar Bawang Bali. To check whether or not a
toxic compound was involved in the resistance present in A. roylei, tenaccessions from four Allium species were screened. Five 3-day old larvae were inoculated on regularly replaced leaf material of each accession ofAllium species. No significant differences were found in mean fresh weight per larva and mean survival of larvae among different
accessions. There were also no significant differences in pupal weight and developmental time. All larvae became pupae 10
days after inoculation. The data indicate that there is no toxic compound present in A. roylei. These results are underlined by the observation in the greenhouse bio-assay that A. roylei plants were equally damaged by the beet armyworm compared to otherAllium species. The results obtained so far therefore suggest that introduction of resistance to S. exigua via the exploitation of variation for resistance to the beet armyworm in A. roylei is unclear and that genetic engineering using Cry sequences could provide a way forward.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
11.
Maize grey leaf spot (GLS) disease remains an important foliar disease in sub-Saharan Africa accounting for more than 25% yield losses in maize. Information on inheritance of GLS resistance of germplasm adapted to African environments is required in new sources being identified. Therefore, hybrids generated from a 10 × 10 half-diallel mating of tropical advanced maize inbred lines were evaluated in six environments to determine combining ability, genotype × environment interaction (G × E) and the impact of GLS disease on grain yield. General combining ability effects were highly significant and accounted for 72 and 68% of the variation for GLS resistance and grain yield, respectively. Significant specific combining ability effects associated with reduced disease levels were observed in some hybrids when one parent was resistant, and these may be exploited in developing single cross maize hybrids. Regression analysis showed a 260–320 kg ha?1 decrease in maize grain yield per each increase in GLS disease severity score, and significant associations (r = ?0.31 to ?0.60) were observed between grain yield and GLS severity scores. This showed the potential of GLS disease to reduce yield in susceptible varieties grown under favourable disease conditions, without control measures. Genotype and genotype × environment biplots and correlation analysis indicated that the significant G × E observed was not due to changes in hybrid ranking, implying absence of a significant crossover interaction. Therefore, predominance of additive gene effects imply that breeding progress for GLS disease resistance would be made through selection and this could be achieved at a few hot-spot sites, such as Baynesfield and Cedara locations in South Africa, and still deploy the resistant germplasm to other environments in which they are adapted. 相似文献
12.
Zenta Nishio Hisayo Kojima Akiyo Hayata Norio Iriki Tadashi Tabiki Miwako Ito Hiroaki Yamauchi Timothy D. Murray 《Euphytica》2010,176(2):223-229
Wheat yellow mosaic, caused by Wheat yellow mosaic virus (WYMV), is one of the most devastating soil-borne diseases of winter wheat (Triticum aestivum L.) in Japan. Yellow-striped leaves and stunted spring growth, symptomatic of WYMV infection, result in severe yield loss.
A new putative WYMV resistance gene in the European wheat cultivar ‘Ibis’ was mapped in the cluster of microsatellite markers
including Xcfd16, Xwmc41, Xcfd168 and Xwmc181 on the long arm of chromosome 2D at the distances of 2.0 cM, 4.0 cM, 7.1 cM and 12.4 cM, respectively. WYMV-resistant cultivars
contained a common haplotype of the four markers, whereas moderately susceptible and susceptible cultivars did not. These
results should be useful in marker-assisted selection for WYMV resistance in wheat. 相似文献