两种世界性仓储虱(虫齿)的重新描述

仓储虱Ｃｈｉ在我国口岸检疫中常有截获。本文针对两种世界性仓储虱Ｃｈｉ,即中国新记录虚伪虱Ｃｈｉ Ｌｉｐｏｓｃｅｌｉｓ ｍｅｎｄａｘ Ｐｅａｒｍａｎ和红虱Ｃｈｉ Ｌ．ｒｕｆａ Ｂｒｏａｄｈｅａｄ,进行了重新描述。２种虱Ｃｈｉ均可危害室内储藏物,分别属ⅡＣ种团和ⅠＢ种团,世界分布较广,我国仅发现于河南省。     

中国虱属分类研究 Ⅳ.常见的几种仓储虱

本文是“中国虱属分类研究”系列论文的第 4篇 ,主要针对 4种世界常见虱属Liposcelis昆虫进行了重新描述 ,即嗜卷虱L .bostrychophilaBadonnel、嗜虫虱L .ento mophila(Enderlein)、无色虱L .decolor(Pearman)和皮氏虱L .pearmaniLienhard。旨在为我国口岸植物检疫 ,特别是有关虱属昆虫的鉴定工作提供参考。     

中国虱属分类研究 Ⅴ.常见的几种仓储虱与植物检疫

本文是“中国虱属分类研究”系列论文的第5篇,首先对世界仓储虱常见种小眼虱LiposcelispaetaPearman进行了重新描述,并给出了5种常见虱检索表;其次对中国虱属已知种进行了总结;最后初步探讨了虱属研究在植物检疫标准化建设中的地位和意义。     

关于鲍氏虱(虫齿)和地虱(虫齿)的再描述

除危害各种储藏物 ,虱属昆虫也可随活体植物、木材、木质包装、废纸、土壤等进行远距离传播。为便于口岸检疫人员及时、准确鉴定所截获的虱属昆虫 ,本文记述了该属 2种中国新记录 ,即鲍氏虱liposcelisbouilloniBadonnel和地虱L .edaphicaLienhard ,并对其进行了再描述     

中国虱属分类研究 Ⅰ.研究历史与现状

虱属昆虫是目中经济意义最重要的一个类群。在我国进境植物检疫中,该属昆虫常有截获,但其种类鉴定存在较大困难。本文作为中国虱属分类研究系列报道之一,在回顾其研究历史的基础上,对３个重要的历史遗留问题进行了讨论。     

中国虱属分类研究 Ⅲ.分类与检索

本文是“中国虱属分类研究”系列论文的第３篇,主要介绍了目分亚目、粉亚目分组、纳组分科、虱科分属以及虱属分种团等５个目和虱属研究中常用的检索表。     

关于鲍氏虱chi和地虱chi的再描述

除危害各种储藏物,虱ｃｈｉ属昆虫也可随活体植物、木材、木质包装、废纸、土壤等进行远距离传播。为便于口岸检疫人员及时、准确鉴定所截获的虱ｃｈｉ属昆虫,本文记述了该属２种中国新记录,即鲍氏虱ｃｈｉ ｌｉｐｏｓｃｅｌｉｓ ｂｏｕｉｌｌｏｎｉ Ｂａｄｏｎｎｅｌ和地虱ｃｈｉ Ｌ．ｅｄａｐｈｉｃａ Ｌｉｅｎｈａｒｄ,并对其进行了再描述。     

瘿木虱属及中国新记录种——木荷瘿木虱和末龄若虫的描述

本文记述了在云南、广西发现的为害银木荷的木荷瘿木虱(Cecidopsyyla schimae Kieffer,1905)及其末龄若虫。并对瘿木虱属进行描述。     

中国虱齿属分类研究：Ⅳ.常见的几种仓储虱齿

本文是“中国虱齿属分类研究”系列论文的第４篇，主要针对４种世界常见虱齿属Ｌｉｐｏｓｃｅｌｉｓ昆虫进行了重新描述，即嗜卷虱齿Ｌ．ｂｏｓｔｒｙｃｈｏｐｈｉｌａ Ｂａｄｏｎｎｅｌ、嗜虫虱瞪Ｌ．ｅｎｔｏｍｏｐｈｉｌａ （Ｅｎｄｅｒｌｅｉｎ）、无色虱齿Ｌ．ｄｅｃｏｌｏｒ（Ｐｅａｒｍａｎ）和皮氏虱齿Ｌ．ｐｅａｒｍａｎｉ ｌｉｅｎｈａｒｄ。旨大为我国口岸植物检疫，特别是有关虱齿有关虫的鉴定工作提供参考。     

书虱及其分子生物学研究进展

书虱（book lice）是目昆虫中有重要经济意义的一个类群,也是世界储粮保护中的常见害虫。本文在概述书虱的危害及其主要种类的基础上,从书虱近似种分子鉴定、分子系统发育、共生菌分子检测与协同进化、种群遗传结构与多态性以及书虱抗性分子机制等几个方面对国内外书虱分子生物学研究现状进行了分析,并就分子生物学技术在书虱领域的进一步研究和应用进行了初步展望。     

Comparison of acetylcholinesterase from three field populations of Liposcelis paeta Pearman (Psocoptera: Liposcelididae): Implications of insecticide resistance

The toxicological and biochemical characteristics of acetylcholinesterases (AChE) in Liposcelis paeta Pearman were investigated in three field populations collected from Nanyang city of Henan Province (NY), Wuzhou (WZ) and Hezhou (HZ) Cities of Guangxi Province, China. The result of bioassay showed that the LC_50s of the NY (281.4802 mg/m²) and the WZ (285.0655 mg/m²) to dichlorvos were 1.156-fold and 1.171-fold higher than that of the HZ (243.5197 mg/m²), respectively. Compared to NY population, the activity per insect and the specific activity of AChE in WZ and HZ populations were significantly higher, and significant kinetic differences among the three populations were also observed. The apparent Michaelis–Menten constant (K_m) for acetylthiocholine iodide (ATChI) was obviously lower in NY than that in WZ and HZ populations, indicating a higher affinity to the substrate ATChI in the NY population. The affinity to the substrate ATChI between WZ and HZ population was also significantly different. As for V_max, the values of WZ and HZ populations were significantly greater when compared to that for NY population, suggesting a possible over expression of AChE in the former two populations. The inhibition studies of AChE indicated that paraoxon-ethyl, demeton-S-methyl, carbaryl, and eserine all possessed some inhibitory effects on AChE in L. paeta. The results of I_50S suggested that when compared to the other two populations, while AChE from HZ population was less sensitive to paraoxon-ethyl and demeton-S-methyl. The contradiction with the result of the bioassay might be due to the different insecticides used in the bioassay. Although both carbaryl and eserine had excellent inhibitory effects, there was no significant difference among the three populations. The statistical analysis of the bimolecular rate constants (k_i) was consistent with the above situation that carbamates expressed remarkable inhibitory effects. It was noticeable that NY population was most sensitive to carbaryl while least to eserine. The differences in AChE among three populations may attribute to the difference in control practices for psocids between Henan and Guangxi Provinces.     

吡丙醚和烯虫酯对嗜卷书虱控制作用的研究

采用饲料混毒法系统测定了吡丙醚和烯虫酯两种昆虫生长调节剂(IGRs)对重要的储藏物害虫嗜卷书虱Liposcelis bostrychophila Badonnel若虫和成虫的致死作用,对若虫发育情况、成虫繁殖力和卵孵化率的影响。结果表明,以吡丙醚和烯虫酯10 mg/kg的混毒饲料饲喂嗜卷书虱2龄幼虫49 d后,若虫的死亡率分别为80.00%和31.11%;取食20、10和5 mg/kg吡丙醚的若虫最长存活天数分别为71.0、98.2和116.4 d,而对照仅11.3 d即全部羽化为成虫;取食20 mg/kg以下烯虫酯混毒饲料的若虫一部分仍可羽化成成虫,而且成虫仍可繁殖后代;取食吡丙醚混毒饲料的若虫,60 d后存活下来的若虫大多以超龄若虫的形式存在,而取食烯虫酯的若虫则很少出现超龄若虫。成虫取食烯虫酯(200 mg/kg)和吡丙醚(40 mg/kg)混毒饲料后7 d,死亡率分别为26.67%和23.33%,成虫产卵量分别是对照的39.77%和26.13%,产卵后15 d,卵的孵化率分别是65.71%和8.69%,而对照仅为93.18%。该项研究表明,采用40 mg/kg吡丙醚和200 mg/kg的烯虫酯对嗜卷书虱虫口数量有明显的控制作用。     

Inhibition of egg development by phosphine in the cosmopolitan pest of stored products Liposcelis bostrychophila (Psocoptera: Liposcelididae)

Phosphine-induced delay in development of eggs was investigated as a mechanism of resistance to this fumigant in Liposcelis bostrychophila Badonnel. One-day-old eggs of a susceptible and a strongly resistant strain of L bostrychophila were exposed to a range of phosphine concentrations for 6days at 30 (+/- 1) degrees C and 70 (+/- 2)% RH. Delay in mean hatching period occurred in both susceptible and resistant eggs, although it was more pronounced in the latter. A maximum delay of 2.65 days was recorded for eggs of the susceptible strain at 0.01 mg litre(-1) (the highest concentration at which eggs survived) and 13.39 days for the resistant strain at 1 mg litre(-1) (the highest concentration tested). Delay in egg development time was positively correlated with increasing phosphine concentration. Our results reveal that the most successful strategy to control resistant L bostrychophila is to apply relatively low concentrations of phosphine for extended exposure times (eg 0.05 mg litre(-1) for 16 days) that allow all eggs to hatch to the much less tolerant nymph stage.     

Potential of piperonyl butoxide‐synergised pyrethrins against psocids (Psocoptera: Liposcelididae) for stored‐grain protection

BACKGROUND: Piperonyl butoxide (PB)‐synergised natural pyrethrins (pyrethrin:PB ratio 1:4) were evaluated both as a grain protectant and a disinfestant against four Liposcelidid psocids: Liposcelis bostrychophila Badonnel, L. entomophila (Enderlein), L. decolor (Pearman) and L. paeta Pearman. These are key storage pests in Australia that are difficult to control with the registered grain protectants and are increasingly being reported as pests of stored products in other countries. Firstly, mortality and reproduction of adults were determined in wheat freshly treated at 0.0, 0.75, 1.5, 3 and 6 mg kg^?1 of pyrethrins + PB (1:4) at 30 ± 1 °C and 70 ± 2% RH. Next, wheat treated at 0.0, 1.5, 3 and 6 mg kg^?1 of pyrethrins + PB (1:4) was stored at 30 ± 1 °C and 70 ± 2% RH and mortality and reproduction of psocids were assessed after 0, 1.5, 3 and 4.5 months of storage. Finally, the potential of synergised pyrethrins as a disinfestant was assessed by establishing time to endpoint mortality for adult psocids exposed to wheat treated at 3 and 6 mg kg^?1 of synergised pyrethrins after 0, 3, 6, 9 and 12 h of exposure. RESULTS: Synergised pyrethrins at 6 mg kg^?1 provided 3 months of protection against all four Liposcelis spp., and at this rate complete adult mortality of these psocids can be achieved within 6 h of exposure. CONCLUSION: Piperonyl butoxide‐synergised pyrethrins have excellent potential both as a grain protectant and as a disinfestant against Liposcelidid psocids. Copyright © State of Queensland, Department of Employment, Economic Development and Innovation, 2010. Published by John Wiley and Sons, Ltd.     

Influence of concentration, temperature and humidity on the toxicity of phosphine to the strongly phosphine-resistant psocid Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae)

BACKGROUND: The psocid Liposcelis bostrychophila Badonnel, is a widespread, significant pest of stored commodities, has developed strong resistance to phosphine, the major grain disinfestant. The aim was to develop effective fumigation protocols to control this resistant pest. RESULTS: Time to population extinction of all life stages (TPE) in days was evaluated at a series of phosphine concentrations and temperatures at two relative humidities. Regression analysis showed that temperature, concentration and relative humidity all contributed significantly to describing TPE (P<0.001, R(2)=0.95), with temperature being the dominant variable, accounting for 74.4% of the variation. Irrespective of phosphine concentration, TPE was longer at lower temperatures and high humidity (70% RH) and shorter at higher temperatures and low humidity (55% RH). At any concentration of phosphine, a combination of higher temperature and lower humidity provides the shortest fumigation period to control resistant L. bostrychophila. For example, 19 and 11 days of fumigation are required at 15 degrees C and 70% RH at 0.1 and 1.0 mg L(-1) of phosphine respectively, whereas only 4 and 2 days are required at 35 degrees C and 55% RH for the same respective concentrations. CONCLUSIONS: The developed fumigation protocols will provide industry with flexibility in application of phosphine.     

Long-term effectiveness of grain protectants and structural treatments against Liposcelis decolor (Pearman) (Psocoptera: Liposcelididae), a pest of stored products

Numerous strains of the psocid pest, Liposcelis decolor (Pearman) were collected from farms and central storages and interbred to form three representative strains from three major grain-growing states of Australia: Queensland, South Australia and New South Wales. These were tested against the grain protectants and structural treatments currently registered for use in Australia. Recently, L decolor has become an important pest of stored grain in Australia, particularly in the eastern and southern parts. There is no published information available on management of this pest and the current pest-management strategy, based predominantly on phosphine fumigation, has failed to control infestations of this pest in numerous grain storages in Australia. Alternative methods of control such as use of contact insecticides were explored in the present work to supplement phosphine fumigation to manage this new pest. From eight grain protectants tested as admixtures, only chlorpyrifos-methyl, bioresmethrin plus piperonyl butoxide, and fenitrothion were found to provide long term (3-9 months) protection against all three strains of L decolor. Chlorpyrifos-methyl gave the best protection, providing a minimum of 7.5 to a maximum of 9 months protection, depending on the strains tested. Three structural treatments, azamethiphos, azamethiphos plus carbaryl and permethrin provided long-term control (8-9 months) of all three strains of L decolor on galvanised steel surfaces, with permethrin delivering 9 months protection against all strains. However, all of these treatments failed to provide long-term control of any strains on concrete surfaces. We conclude that chlorpyrifos-methyl as a grain admixture can be incorporated into fumigation strategies to optimise the control of L decolor infestations. Structural treatments, such as permethrin can be used to support a fumigation strategy in storages made of galvanised steel.     

Two new records of downy mildews (Peronosporaceae) in Turkey and a new host

Peronospora crucianellae Maire onCrucianella latifolia L. andPeronospora trifolii-arvensis Syd. onTrifolium sp. were collected for the first time in Turkey. The plant speciesSinapis arvensis L. is reported as a new host forPeronospora brassicae Gäum. from Turkey. The peronosporaceous fungi recorded since the beginning of microfungal studies from Turkey are given along with their collection sites.     

Toxicological and biochemical characterizations of AChE in Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae)

The toxicological and biochemical characteristics of acetylcholinesterases (AChE) in the resistant and susceptible strains (SS) of Liposcelis bostrychophila were investigated. The two resistant strains were the dichlorvos-resistant strain (DDVP-R) and the phosphine-resistant strain (PH₃-R) with resistance ratios of 22.36 and 4.51, respectively. Compared to their susceptible counterpart, the AChE activity per insect and the specific activity of AChE in DDVP-R and PH₃-R were significantly higher. There were also significant kinetic differences between DDVP-R and PH₃-R. The apparent Michaelis-Menten constant (K_m) for acetylthiocholine iodide (ATChI) was obviously lower in SS than that in PH₃-R, indicating a higher affinity to the substrate ATChI in the susceptible strains. The affinity for the substrate ATChI in DDVP-R and SS were not significantly different. The V_max value of the PH₃-R was significantly greater when compared to the V_max for the SS suggesting a possible over expression of AChE in this resistant strain. The inhibition of AChE to insecticide exposure in vitro revealed that all six insecticides were inhibitory for the extracted AChE’s. Based on the I₅₀ values, AChE of the SS were more sensitive to dichlorvos, paraoxon-ethyl, malaoxon and demeton-S-methyl than those of the two resistant strains. As for carbaryl and eserine, the PH₃-R suggested a significantly higher I₅₀s compared to the susceptible strain, while, no significant differences were found between SS and DDVP-R.