pGRF基因质粒对猪胴体性状和肌肉品质的影响

为研究猪生长激素释放因子(pGRF)基因质粒对猪胴体性状和肌肉品质的影响,试验选用24头大白×长白二元杂交去势公猪平均体重(17.25±0.62)kg,随机分为4个处理,每个处理6个重复,分别经臀部注射0、4.0 mg、6.0 mg和8.0 mg的pGRF基因质粒。结果表明:注射4.0 mg和6.0 mg的pGRF基因质粒有提高猪宰前活重、眼肌面积、屠宰率、胴体直长、胴体斜长及降低背膘厚的趋势,其中6.0 mg组宰前活重和胴体斜长与对照组相比差异显著(P0.05)。注射pGRF基因质粒对猪肌肉品质无显著影响(P0.05)。     

注射pGRF基因质粒对由脂多糖或三联疫苗诱导的免疫应激仔猪生长和免疫指标的影响

为研究猪生长激素释放因子(pGRF)基因质粒对由脂多糖或三联苗诱导的免疫应激仔猪生长性能和免疫指标的影响,选用36头(28±2)d断奶的杜长大(Duroc×Landrace×Yorkshire,DLY)三元杂交仔猪,按体重相近、阎公猪和母猪各1/2的原则,分为对照组(不注射pGRF基因质粒、大肠杆菌LPS或三联苗)、注射pGRF组、注射脂多糖(LPS)组、注射pGRF+LPS组、注射三联疫苗组(猪瘟兔化弱毒,猪丹毒杆菌G4T10弱毒,猪源多杀性巴氏杆菌E0630弱毒)和注射pGRF+三联疫苗6个处理,每个处理6个重复,每个重复1头猪.试验开始时,pGRF组、pGRF+LPS组、pGRF+三联疫苗组的仔猪大腿肌肉分别注射1.0 mg pGRF基因质粒;试验的第11天给LPS组、pGRF+LPS组仔猪注射100 ug/kg LPS生理盐水溶液,三联疫苗组以及pGRF+三联疫苗组注射2头份三联苗生理盐水溶液,对照组和pGRF组注射生理盐水;第18天时按照第11天的操作重复进行.结果表明,在2次注射LPS或三联苗后,即10～17 d和17～24 d阶段,pGRF+LPS组仔猪的ADG显著(10～17 d,P<0.05)或极显著(17～24 d,P<0.01)高于LPS组,F/G均显著低于LPS组(P<0.05);pGRF+疫苗组仔猪ADG均显著高于疫苗组(P<0.05),F/G显著低于疫苗组(P<0.05).在第11天和第18天,pGRF+LPS组仔猪的血清细胞因子(IL-1、IL-6)水平均极显著低于LPS组(P<0.01),类胰岛素生长因子(IGF-I)和IgG浓度均极显著高于LPS组(P<0.01);pGRF+疫苗组仔猪的血清IL-1和IL-6浓度显著低于疫苗组(P<0.05),lgG浓度极显著(11 d,P<0.01)或显著(18 d,P<0.05)高于疫苗组,IGF-I浓度显著(11 d,P<0.05)或极显著(18 d,P<0.01)高于疫苗组.证明pGRF基因质粒能缓解由LPS或三联疫苗免疫应激引起的仔猪生长抑制;能够抑制由脂多糖或三联苗诱导的血清IL-1和IL-6浓度的上升和血清IGF-I浓度的降低,同时能提高免疫应激仔猪的血清IgG浓度.     

肌肉注射高剂量pGRF基因质粒对猪生产性能及血液激素水平影响的研究

为探讨高剂量注射pGRF基因质粒对动物生产性能及血液激素水平的影响,本试验选取平均体重为(6．78± 0．47)kg的断奶仔猪24头,随机分为3个处理,每个处理4个重复。在试验开始时按不同处理分别注射0、2．2 mg和 4．4mg的pGRF基因质粒。结果表明:注射2．2 mg和4．4 mg的pGRF基因质粒对动物的生产性能具有一定的改善作用(P<0．05或P>0．05),注射高剂量(4．4 mg)pGRF基因质粒相对于2．2 mg剂量并不能进一步改善动物的生产性能。高剂量(4．4 mg)pGRF基因质粒可相对延长其在动物体内的作用时间,可有效提高动物生长后期血浆GRF浓度。高剂量(4．4 mg)的pGRF基因质粒具有促进内源生长抑素(SS)分泌的作用,因而不利于其生物学效应的发挥。     

恩诺沙星在猪组织中残留消除规律研究

在常规饲养条件下,对健康猪按2.5 mg/kg体重的剂量肌肉注射2.5%恩诺沙星注射液,每日2次,连续注射3 d.停药后第2、4、6、8、10、12、14天分别屠宰4头猪.分别采取每头猪的肌肉(注射部位)、脂肪(腹脂)、肝和肾脏等4种组织,用高效液相色谱法进行残留量测定.结果表明:残留在肌肉、脂肪组织中的药物消除较快,第8天总残留量(恩诺沙星 环丙沙星)已下降至检测限(20μg/kg)以下;肝和肾脏组织中的药物消除缓慢,第14天测得猪肾中药物总残留量为40μg/kg.     

pGRF基因质粒对免疫应激断奶仔猪生长抑制的缓解作用

旨在研究pGRF基因质粒对免疫应激断奶仔猪生长的影响,探讨pGRF基因质粒缓解免疫应激引起的生长抑制作用及其可能的机理。选取18头日龄为（35&#177;2）d、体质量为（7.86&#177;0.59）kg未去势的杜洛克&#215;长白&#215;大白（DLY）三元杂交断奶仔猪,采用单向分类试验设计,按体质量相近、性别一致的原则配对,分为3个处理,即pGRF（pGRF基因质粒）组、pGRF＋LPS（脂多糖）组和LPS组,每个处理6个重复,每个重复1头猪。结果表明：pGRF基因质粒可以缓解因注射LPS导致的日增质量的降低（P〈0.05）,饲料转化率有所改善（P〉0.05）;能够抑制由LPS诱导的细胞因子（IL-1I、L-6）浓度的上升（P〈0.05或P〈0.01）和血清IGF-I浓度的降低（P〈0.05或P〈0.01）,同时显著提高血清IgG的浓度（P〈0.05）。结论：pGRF基因质粒缓解了断奶仔猪因注射脂多糖引起的生长抑制,降低了血清IL-1和IL-6的水平,提高了血清IgG的浓度,提高了仔猪的抗病力,从而缓解了断奶仔猪的免疫应激;pGRF基因质粒缓解免疫应激的作用与其促进IGF-I的分泌密切相关。     

生长肥育猪骨骼肌注射表达pGRF基因质粒的效应研究

将猪的GRF基因表达质粒注射于猪的骨骼肌后,研究其促生长效应与机理。选用始重6.3kg的44头去势长白×太湖仔猪,分6组,采用2×3因子安排的完全随机区组设计,按6～10kg、10～20kg、20～50kg、50～100kg4个阶段饲养。4个饲养阶段的饲粮低蛋白水平分别是20.70%,17.90%,15.03%,13.00%;高蛋白水平分别是23.70%,20.90%,18.02%,16.00%。pGRF基因质粒注射剂量设0mg、0.5mg、1.0mg3个水平,于试验开始与试验猪体重达60kg时共注射基因质粒2次。测定各阶段日增重,饲料消耗量,耗料增重比以及30、70、100kg时血液中GH、GRF、IGF-I的浓度。100kg时屠宰进行胴体品质测定。结果表明:饲粮蛋白水平对各阶段及全期试验猪日增重均有显著影响(P<0.05或P<0.01),对50～100kg阶段与全期日采食量和耗料增重比有显著影响(P<0.05或P<0.01)。注射pGRF基因质粒对各阶段及全期日增重均有显著影响(P<0.05),对6～10kg、10～20kg、50～100kg3阶段及全期日采食量有显著影响(P<0.05),对6～10kg阶段、50～100kg阶段及全期耗料增重比有显著影响(P<0.05)。注射pGRF基因质粒对30kg及70kg体重时猪血液中GRF、GH、IGF-I浓度均有显著影响(P<0.05)。提高饲粮蛋白水平与注射pGRF基因质粒均可明显降低超声波测膘厚及屠宰测膘厚、增大眼肌面积(P<0.05)。     

长效盐酸多西环素注射液在猪体内残留的消除规律

在常规饲养条件下,对35头健康成年猪按10 mg/kg体质量的剂量肌肉注射10%长效盐酸多西环素注射液,给药2次,给药间隔时间为48 h。第2次给药后12 h及2、5、9、14、192、5 d分别屠宰5头猪,分别采取每头猪的肌肉、肝脏、肾脏、皮肤+脂肪和注射位点肌肉等5种组织,用高效液相色谱法进行残留量测定。结果表明:在第2次给药后19 d,多西环素在各组织均能检测到,且残留均低于残留限量。多西环素残留浓度大小顺序:注射部位(肾脏(肝脏(皮脂(肌肉。采用WT1.4软件制定的统计方法来处理猪组织中药物浓度-时间数据,以制定休药期。     

注射pGRF基因质粒对猪血清生长轴激素变化规律的影响

本研究旨在探讨注射pGRF基因质粒在猪体内的表达效应及作用规律.试验选择8头体质量相近((15 0.43)kg)的健康长白×大白二元杂交阉公猪,随机分成2组.试验组颈部肌肉注射含4.5 mg pGRF基因质粒的注射液2 mL,对照组注射生理盐水2 mL.于处理后第1、4、7、11、16、21、26、31天空腹采血样制备血清,测定血清中生长激素(GH)、类胰岛素生长因子(IGF-I)、生长激素释放因子(GRF)、生长抑素(SS)的含量.结果表明:试验组在注射pGRF基因质粒后第7天,血清GH含量达峰值,比对照组高了113.47%,差异极显著(P<0.01);然后逐渐下降.到第11天时,比对照组高47.09%,差异显著(P<0.05).到第31天时,与对照组基本一致.试验组血清IGF-I和GRF含量虽然提高幅度不大,但其变化与GH含量存在着显著相关性,均在注射pGRF基因质粒后第7天达到峰值,然后降至对照组水平,其相关系数分别为0.589(P<0.05)和0.678(P<0.05).与对照组相比,试验组血清SS含量在11 d前一直呈下降趋势,第11天时降至最低点,但差异不显著.到第16天时2组均有所上升,21 d后缓慢下降,第31天时与对照组趋于一致.血清SS含量与GH、GRF的含量分别存在显著和极显著负相关,相关系数分别为-0.689(P<0.05)和-0.777(P<0.01).由此可见,pGRF基因质粒在体内快速表达并作用于机体,主要通过提高GRF的含量而促使GH的分泌,同时抑制SS的分泌,一定程度阻止其对GRF和GH的抑制作用,从而达到有效的促生长作用.同时本试验发现,虽然采用了颈静脉导管方法,猪血液中生长轴激素测定值变异仍较大,加强护理和增加测试猪的数量是取得更为客观结果的必要条件.     

N-乙酰半胱氨酸对脂多糖刺激仔猪小肠黏膜免疫应激的影响

研究N-乙酰半胱氨酸(NAC)对脂多糖(LPS)刺激仔猪免疫应激的影响。选取18头健康仔猪,随机分成3个处理(对照组、LPS组和NAC组),每个处理6个重复。对照组和LPS组饲喂基础日粮,NAC组饲喂基础日粮+500 mg/kgNAC。试验期为20 d。LPS组和NAC组于试验第10、13、20天腹膜注射100μg/kg BW的LPS,对照组注射相应剂量的灭菌生理盐水。第10、20天注射LPS后3 h采血,第21天屠宰,取小肠黏膜。结果表明:NAC缓解了LPS刺激导致的血浆和小肠黏膜中TNF-α、IL-6、PGE2含量的升高(P<0.05),缓解了小肠黏膜HSP70相对表达量的升高(P<0.05)。由此可见,日粮中添加500 mg/kg NAC可有效抑制LPS刺激导致的血浆及小肠黏膜中炎性因子的升高,缓解免疫应激。     

猪生长激素释放因子基因质粒对猪生长发育的影响

试验选用 30头杜洛克×太湖和汉普夏×太湖二元杂仔猪 ,平均 4 4日龄断奶 ,体重 (10 .30± 1.80 )kg ,研究了经腿部肌肉注射 0 ,0 .2 5 ,0 .5 ,1.0和 2 .0mg猪生长激素释放因子 (pGRF)基因质粒对猪生产性能、胴体品质和血液参数的影响。试验结果表明 ,pGRF基因质粒对猪不同生长阶段生产性能的影响不同 ,10～ 4 0kg阶段促生长效果好于 4 0～ 10 0kg阶段 ,且促生长效果与剂量有关。相对于促生长效果 ,在猪整个生长阶段pGRF基因质粒对饲料利用率的改善效果更明显。 10 0kg体重屠宰 ,2mg组对胴体品质改善最明显 ,但对肉质无影响 ;血液中GH、IGF I、GRF及血糖浓度和血清尿素氮与对照组均无显著差异     

CREB-H基因在猪不同组织中表达谱及肝脏中的表达规律

本研究旨在分析环腺苷酸应答元件结合蛋白H（cyclic AMP-responsive element-binding protein 3-like 3，CREB-H）在猪不同组织中的表达谱及其在马身猪和大白猪肝脏中的发育性表达规律。采用实时荧光定量PCR和Western blotting技术检测1日龄猪12个组织（心脏、肝脏、脾脏、肺脏、肾脏、胃、小肠、小脑、下丘脑、背最长肌、股肌和腰肌）中CREB-H基因的表达谱，以及CREB-H在1、30、60、90、120、150和180日龄马身猪和大白猪肝脏中的表达规律。结果显示，CREB-H基因mRNA在马身猪的12个组织中广泛表达，其中在肝脏和小肠中高表达；CREB-H蛋白在肝脏组织中的表达量显著高于其他组织（P<0.05），在心脏、脾脏和小脑中不表达。猪肝脏CREB-H基因mRNA和蛋白的发育表达受日龄、品种、品种与日龄相互作用的影响（P<0.01）。马身猪和大白猪肝脏中CREB-H基因mRNA和蛋白的表达量均在1日龄时达到最大值。在各发育阶段，马身猪CREB-H蛋白的表达量均极显著高于大白猪（P<0.01），且CREB-H主要在猪肝脏中表达。CREB-H在两猪种肝脏中的表达存在时空差异，可能与猪在不同发育期的脂质代谢能力有关，本试验结果为研究猪的脂质代谢调控机制提供一定的理论依据。     

MYNN基因在猪不同组织及肌肉中的表达规律

试验旨在研究myoneurin（MYNN）基因在猪不同组织中的表达特征及其在肌肉（背最长肌、股二头肌和腰大肌）、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的发育性表达规律。采用实时荧光定量PCR技术研究猪MYNN mRNA在90日龄大白猪和马身猪的心脏、肝脏、脾脏、肺脏、肾脏、小脑、小肠、胰脏、胃、股二头肌及脂肪共11个组织中的表达谱,以及在大白猪和马身猪1、90、180日龄3个发育阶段的肌肉、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的发育性表达规律。结果表明,MYNN在猪的各种组织中广泛表达,且各组织间表达差异显著或极显著（P < 0.05;P < 0.01）;MYNN在大白猪和马身猪的肌肉、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的不同发育阶段表达差异显著或极显著（P < 0.05;P < 0.01）,并具有特定规律,由此推测其可能在猪的这几种组织中发挥重要作用。MYNN基因的表达与组织、日龄及品种的遗传背景有关。本试验为研究猪MYNN基因的生物学功能提供了依据,但还需要深入的研究来探索其作用的具体机制,尤其是在骨骼肌发育中的调节机制。     

The Expression Characteristics of MYNN Gene in Different Tissues and Muscles of Pig

This study was aimed to investigate the expression characteristics of myoneurin (MYNN) gene in different tissues and its developmental expression in muscles (longissimus dorsi, biceps femoris and psoas major), cerebellum, liver, pancreas, kidney, stomach, spleen and lung tissues of pigs. The expression characteristics of MYNN mRNA in 11 different tissues including heart, liver, spleen, lung, kidney, cerebellum, small intestine, pancreas, stomach, biceps femoris and fat of Large White pigs and Mashen pigs at the age of 90 days and the developmental expression patterns in muscle, cerebellum, liver, pancreas, kidney, stomach, spleen at three strages (1, 90, 180 days) of Large White pigs and Mashen pigs were studied by Real-time PCR. The results showed that MYNN was widely expressed in various tissues of pigs, and there was significant difference among the tissues(P < 0.05; P < 0.01); The expression of MYNN in muscle, liver, pancreas, cerebellum, kidney, stomach, spleen, lung tissues were significant difference at three development stages of Large White pigs and Mashen pigs(P < 0.05; P < 0.01), and also had a specific rule, which indicated that it may play an important role in these pig tissues. The expression of MYNN gene could related to the tissue, age and the genetic background of breeds. The results of this study provided a better understanding of the biological functions of pig MYNN. Further studies are required to determine its molecular mechanisms, especially in the regulation of skeletal muscle development.     

猪肾脏等组织中盐酸莱克多巴胺的HPLC检测方法及其残留消除

本研究建立了猪肝脏、肾脏、肌肉和脂肪中盐酸莱克多巴胺的高效液相色谱检测方法。方法的检测限为1ng/g,定量限为2 ng/g,肝脏的平均回收率在73.4%～83.2%,变异系数在2.1%～6.6%;肾脏的平均回收率在73.1%～95.5%,变异系数在3.8%～4.0%;肌肉的平均回收率在80.7%～82.5%,变异系数在4.9%～9.1%;脂肪的平均回收率在73.3%～78.8%,变异系数在2.9%～6.7%。对60头健康猪以18 mg/kg的剂量混饲给药28d,停药饲喂14 d。在给药7、142、8 d和停药1、2、3、7、9、14 d分别屠宰6头猪,取各组织进行残留量测定。结果显示：肾脏中残留量最高,肝脏其次,残留量在停药期间较给药期间显著降低。其中肾脏和肝脏中的残留量分别在停药14 d、停药9 d后降至定量限以下;肌肉和脂肪中的残留量显著低于肾脏和肝脏,给药28 d时,残留基本低于定量限;停药1 d时均低至检测限以下。     

猪生长激素释放因子基因对早期断奶仔猪生产性能、免疫功能及消化道发育的影响

为探讨注射猪生长激素释放因子基因 (pGRF)对早期断奶仔猪生产性能、肠粘膜及免疫功能的影响 ,选取 4周龄杜长大同窝断奶仔猪 8头 (6 .2 7± 1 .1 9kg) ,等分为两组 ,日粮分高、低两个水平 ,做一对比试验 ,试验期 2 1天。结果表明 :断奶后注射组仔猪ADG第 1、2周及 1～ 2周分别增加 2 8.57g、2 5 .0 1g和 2 6 .79g ;饲料转化率 1、2周分别提高 33 .80 %和 1 7.33 % ;N沉积率第 1、2及 1～ 2周分别增加 3 .67%与 5 .1 3 %和 1 6 .96 % ;绒毛长度增加 1 9.71 % ;血清IgG含量在断奶时、第 1周末和第 2周末分别增加 3 .82 %、9.90 %和 8.1 9%。     

Tissue retention and adverse reaction after intravenous injection of hematoporphyrin derivatives in dogs

We evaluated changes in hematology and chemical profile, and the tissue retention of hematoporphyrin derivative (HpD) following the intravenous injection in dogs. HpD at concentrations of 1, 5, 10, and 15 mg/kg was intravenously injected to 16 dogs (n=4 each) and complete blood count (CBC) and blood chemistry were performed on days 1, 3, 5, and 7 after the injection. To examine tissue retention, HpD (5 mg/kg) was administered to 15 dogs and 3 dogs were euthanized on days 1, 2, 7, 14, and 28 after the injection, respectively, to collect the skin, muscle, small intestine, spleen, kidney and liver as tissue samples. There were no significant changes in CBC and blood chemical profile except for an increase in LDH concentrations in dogs given 10 and 15 mg/kg of HpD at day 3. The levels of HpD retention in the tissues were ranked in the following order: liver > kidney > spleen > intestine > muscle > skin.     

Evaluation of fenbendazole as an extended anthelmintic treatment regimen for swine

Fenbendazole was given in the feed to swine at a cumulative dosage of 9 mg/kg of body weight over a period of 3, 6, and 12 days to compare efficacy. Four treatment groups of ten 2- to 3-month-old pigs each, with a mean of 15 kg of body weight per group, received 3 mg of fenbendazole/kg/day for 3 days, 1.5 mg/kg/day for 6 days, 0.75 mg/kg/day for 12 days, and no medication. Medicated feed was scheduled so that all treated pigs reached the last day of treatment on the same day, thus making the time between the last treatment and necropsy equal for all groups. Ascaris suum and Trichuris suis were the target species, their presence before treatment being determined by fecal egg counts and at necropsy by worm counts. At necropsy, 9 control pigs were infected with A suum (mean of 18.0 worms/pig), and all control pigs had T suis infection (mean of 36.5 worms/pig). All 3 treatment schedules were 100% effective in removal of A suum; and for T suis, the 3-day regimen was 100% effective, the 6-day regimen, 99.2%, and the 12-day regimen, 91.0%.     

Multiorgan engraftment of human somatic cells in swine foetuses after intra-blastocyst transplantation

Adult human stem cells, mainly from hematopoietic lineage, have been injected into developing pre-immune animal foetuses, and xenogenic engraftment of liver and other organs has been reported. We isolated a rare cell population from adult human liver, fat and skin. Colonies with few cells became visible as early as 2-3 days, and a fully formed colony took 10-14 days to form. These colonies were named as liver-derived cell lines (LDCs), fat-derived cell lines (FDCs) and skin-derived cell lines (SDCs). All these cells express few pluripotency markers like Klf4, c-myc and Sox2. Pig blastocysts were injected with LDCs, FDCs and SDCs and transferred to recipient pigs. We achieved an overall pregnancy rate of 71.4% at day 35. The foetuses were analysed for human cell chimerism in liver, kidney and heart both by RT-PCR and real-time PCR using primers specific to human and pig mitochondrial DNA. The percentage of foetuses showing chimerism was 17.4% (4/23), 12.5% (2/16) and 11.1% (1/9) for LDCs, FDCs and SDCs, respectively. Of these, 42.9% (three out of seven) showed chimerism in liver and 71.4% (five out of seven) showed kidney chimerism. However, we did not detect any chimerism in the heart. The level of chimerism varied and was in the range of one human cell per one hundred thousand to one million pig cells.     

Experimental production of porcine gastroesophageal ulcers by betazole and reserpine

The ulcerogenic and secretogenic properties of betazole, (an H₂ agonist), and/or reserpine on pig stomachs were studied. (1) Betazole (50 mg/body) and reserpine (0.02 mg/kg) were successively injected (i.m.) for 5–10 days into starved animals. Gastroesophageal ulcers, pathologically similar to naturally occurring ulcers were observed in all pigs (10/10), whether a nutrient solution (5% glucose, 1 litre/day) was supplied or not. In the starved pigs given either betazole or reserpine, only a small ulcer or erosion was observed. (2) In four pigs which were given commercial mash at a rate of 3% of body weight per day and killed on the 6th or 10th day of successive injections of both drugs, the incidence of ulcers was 2/4. (3) The gastric secretogenic effect of betazole and/or reserpine was confirmed in pigs with Heidenhain gastric pouches. (4) From these data, a new method for the experimental induction of pig gastroesophageal ulcers, by means of simultaneous injections of betazole and reserpine for 5 days under the nutrient (glucose solution) drinking condition, is proposed. The authors consider that this method may be superior to the repository histamine method, with respect to the absence of adverse effects, practical convenience and pathological and etiological similarities with naturally occurring ulcers.     

饲粮铁、铜水平对早期断奶仔猪生长发育及组织铁、铜含量的影响

本试验就饲粮铁、铜水平对早期断奶仔猪(30日龄)生长发育及组织中铁、铜含量的影响进行了研究。结果指出:在含铁70mg/kg及含铜10mg/kg的基础饲料中以硫酸亚铁形式添加35及70mg/kg铁和以硫酸铜形式添加125及200mg/kg铜时,对仔猪生长性能、组织器官的发育以及品骨、肾脏中的铁含量没有影响,但饲粮铁水平的增加可导致肝脏和脾脏铁含量的增加。在饲粮中添加35mg/kg及70mg/kg的铁时,可使肝脏铁含量增加14.6％和55.0％(P<0.01),使脾脏铁含量增加15.1％(P<0.05)及22.9％(P<0.01)。在仔猪饲粮中添加200mg/kg铜,可使仔猪日增重和采食量分别提高9.7％和16.2％,使肾脏和肝脏铜含量分别增加104.6％(P<0.01)和434.0％(P<0.01),使肝脏铁含量降低19.4％(P<0.05)。添加125mg/kg铜,使肾脏和肝脏铜增加27.9％和93.4％(P<0.01),使肝脏铁降低9.4％。