仔猪温和型猪瘟的诊治

2003年以来,我县一些规模化养殖场中时有仔猪不明原因大量死亡。通过笔者的调查和检疫,发现出现上述死亡的原因60%以上为温和型猪瘟,现举一例:1发病情况2005年3月20日,柏城乡某生猪繁殖场,30日龄已断奶仔猪190头(于20日龄时注射过5倍猪瘟疫苗),陆续出现下痢症状,曾用长效治菌磺     

仔猪副伤寒的诊治经过

病例介绍 我县某饲养场２０００年７月１８日从邱县集市进购仔猪５１头，２５日从山东聊城又进购仔猪７６头，第二批进场两天后统一注射了猪三联苗，当时即有２５头猪拉稀，用庆增安、磺胺嘧啶钠及氟哌酸治疗后，症状见轻。停药３天后，又有２６头发病，３天内死亡９头。后到某兽医站诊断，定为猪瘟伴发仔猪副伤寒。取速灭杀星、克拉稀、消炎热等药物治疗，同时全群紧急注射猪瘟免化弱毒苗，使用上述方法病情仍没有得到有效控制，又死亡１０头。后又到另一个体兽医诊所诊冶，判定为仔猪副伤寒。取海达注射液肌注，同时口服仔猪副伤寒疫苗，…     

一起耐药性猪大肠杆菌病的治疗和体会

1病情及临床症状 2013年4月2日，谭店乡一养殖户，新购仔猪98头，平均体质量10奴。回家后7d开始出现零星拉稀，第3天开始大面积出现拉稀现象，死亡2头。随用“痢菌净”注射、氟哌酸拌料，经3d治疗，大部分仔猪不再拉稀。又过10d，发现有5头仔猪不愿活动，食欲下降，粪便干结，体温37℃～37．5℃，用樟脑磺酸钠注射液进行注射，     

非典型猪瘟的诊疗体会

近年来，不同规模的养猪场中非典型猪瘟（又称温和型猪瘟）发病率呈上升趋势。今年上半年，笔者共收治非典型猪瘟１０８例，其中仔猪５６头，青年猪４８头，母猪４头，经过综合治疗，取得了较好的效果。１临床症状１．１仔猪发病初期体温升高到４１～４２．３℃，经用退热药、抗菌素或磺胺类药物治疗后仍稽留在４０．２～４０．５℃左右。精神萎顿，喜卧堆叠，鼻盘干燥，部分病猪鼻孔流出水样清涕。不食，粪干小呈栗子状。有的粪过，症状轻且不典型，体温稍高，多在３９．８～４０．５℃之间。食欲大减，精神不振，有的不需任何药物治疗，半…     

建议仔猪市场防疫注射时每头换一个针头

随着兽医工作的普及,各地市场对仔猪检疫的同时进行防疫注射。笔者曾碰到这样的情况,一次上市买仔猪,经检查体温、食欲、外表体态均正常,但经买回家后第四天就发病了,且猪瘟症状很明显。后来回忆这头仔猪在市场检疫注射时兽医员给仔猪防疫     

引起猪便秘、肠阻塞症状的常见病的诊断

1猪瘟 猪的急性、热性、接触传染性疾病,由猪瘟病毒引起。病猪体温升高,病初便秘,随后下痢,有时呕吐。少数病猪可发生惊厥,常在数小时或数天内死亡。除急性型外,尚有慢性型、迟发型和温和型猪瘟。2伪狂犬病本病是由伪狂犬病毒引起的急性传染病。临床表现为体温升高,新生仔猪表现神经症状,消化紊乱,2周龄内哺乳仔猪以发热、呕吐、下痢、厌食,间歇性痉挛,后躯麻痹为特征。     

一起猪流行性腹泻病毒和猪链球菌混合感染引发仔猪拉稀病例的诊断及分析

为确诊广西一存栏1000头母猪的某规模养猪场2耀3日龄仔猪临床持续表现严重拉稀症状的病因,研究对拉稀仔猪进行了临床诊断和实验室诊断。对拉稀仔猪的发病情况调查,临床症状和病理变化观察,实验室细菌分离培养及鉴定,进行PCR/RT-PCR方法检测可能引起仔猪腹泻的常见疫病病毒(如猪瘟病毒、猪蓝耳病病毒、猪伪狂犬病病毒、猪圆环病毒2型和猪圆环病毒3型)以及常见肠道腹泻病毒(流行性腹泻病毒、猪传染性胃肠炎病毒、猪轮状病毒和猪丁型冠状病毒)。结果在2窝拉稀仔猪肠系膜淋巴结中均分离到猪链球菌2型(S.s2),同时在拉稀仔猪肠道中均检测到猪流行性腹泻病毒(PEDV),而其它病原检测均为阴性,表明S.s2和PEDV混合感染可能是引起本次仔猪拉稀病例的主要原因。     

仔猪猪瘟的诊断与防控技术

仔猪是发展养猪业的基础,仔猪的养殖在提高单位区域内猪只数量和密度的同时,也提高了仔猪的病毒性疾病感染率和死亡率,尤其是仔猪猪瘟。仔猪猪瘟有典型性、非典型、温和型猪瘟3种形式。由于非典型、温和型猪瘟的临床症状不明显,不易诊断。因此本文介绍了引起仔猪猪瘟原因、流行特点、诊断及防治措施。     

两起猪瘟的防制报告

一、基本情况 今年３月６日笔者应邀到一小型养猪场协助诊断猪病。经了解该猪场原存栏７５千克左右育肥猪４０头，２月１６日从某市场购入２５千克左右的仔猪３６头，２月１７日对新购入的仔猪注射了猪瘟疫苗，１头份／头，２月２５日新购入的猪群开始发病，并逐渐出现死亡，到３月６日相继死亡９头。经检验病猪体温升高可达４１℃，拉稀或拉稀便秘交替，耳部、臀部发紫，四肢内侧及腹下有出血点。剖检死猪可见全身淋巴结周边出血，切面如大理石样花纹，肾脏表面有出血点，心耳、心肌有条索状或点状出血，胃黏膜、小肠黏膜有出血，有的膀胱…     

仔猪温和型猪瘟的全治

2003年以来，我县一些规模化养殖场中时有仔猪不明原因大量死亡。通过笔者的调查和检疫，发现出现上述死亡的原因60％以上为温和型猪瘟，现举一例：     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.