早熟丰产节瓜新品种冠华7号的选育

冠华7号节瓜是以强雌系mbD2-2-2为母本、fbD-4为父本配制成的杂种1代,为七星类节瓜。该品种生育期露天栽培从播种至初收春季约70 d、秋季约52 d,延长采收期春季约34 d、秋季约35 d,全生长期春季约104 d、秋季约87 d;丰产性好,第1朵雌花着生节位7.3～9.6节,第1个瓜坐瓜节位8.3～11.0节。商品瓜呈短圆筒形,瓜皮深绿色,无棱沟。单瓜质量583.3～599.2 g,瓜长18.5～18.6 cm,横径7.3～7.6 cm,果肉厚度1.43～1.50 cm。果肉致密、品质优,粗蛋白含量(w,后同)0.38%,维生素C含量220 mg·kg^-1,可溶性固形物含量5.2%。单株产量2.32～2.54 kg,平均667 m²产量3250 kg,适合华南地区及北方种植。2020年通过广东省农作物品种审定委员会评定。     

节瓜新品种‘花玉女’的选育

‘花玉女’节瓜是以‘甜宝’节瓜作母本、‘梅花’节瓜作父本配制而成的杂交1代品种,为江心类节瓜。露地栽培种植全生育期春季106 d、秋季91 d;从播种至始收春季70 d、秋季56 d,延续采收期春季36 d、秋季35 d,第1朵雌花着生节位9.5~11.2节,第1个瓜坐瓜节位10.0~11.9节。瓜呈长圆筒形,瓜皮青绿色,无棱沟,花点稍大且数量相对较多、麻点明显,肉质致密。瓜长22.3~22.6 cm,横径6.94~7.18 cm,肉厚1.79~1.87 cm,单瓜质量667.2~704.1 g,单株产量1.84~2.45 kg,667 m^2产量3653~3919 kg,商品率高,品质优,粗蛋白质量分数0.496%,维生素C质量分数212 mg·kg^-1,可溶性固形物质量分数3.0%。田间表现耐热性、耐寒性、耐涝性和耐旱性强。适合华南地区春秋季栽培。2019年通过广东省农作物品种审定委员会审定。     

有棱丝瓜新品种桂冠5号的选育

桂冠5号是以自交系B05-13-2为母本,以自交系A10-12-3-7为父本配制而成的有棱丝瓜一代杂种.植株生长势强,分枝能力强,主、侧蔓均可结瓜,叶片深绿色;早熟,第1雌花节位春季为第12～13节、秋季为第16～18节,播种至始收春季65～68 d(天)、秋季45～47 d(天);商品瓜短棒形,瓜皮绿色带斑点,瓜长3...     

2015年广东省蔬菜审定品种

<正>红樱1号番茄选育单位:广州市农业科学研究院特征特性:一代杂种。无限生长类型,植株生长势强。从播种至始收春季103 d(天)、秋季94 d(天),全生育期春季138 d(天)、秋季145 d(天)。第1花序节位为第9.0~9.6节,第1花序坐果率75.51%~82.60%,整株坐果率77.00%~83.02%。果实椭圆形,无绿果肩,转色均匀,熟果鲜红色,果面光滑、无棱沟,纵环裂轻。樱桃型中果,单果     

节瓜新品种吉宝1号的选育

吉宝1号是以自交系黑毛为母本,以自交系绿丰为父本配制而成的七星类节瓜一代杂种。早中熟,播种至始收春季69 d(天)、秋季54 d(天),延续采收期春季36 d(天)、秋季33 d(天)。瓜圆筒形,瓜皮深绿色,无棱沟,花点中等大小且分布较均匀,肉质致密,品质优良;瓜长19 cm左右,横径7.5 cm左右,肉厚1.7 cm左右,单瓜质量600～720 g,商品瓜率95%左右;VC含量219.0 mg · kg~(-1),可溶性固形物含量36.0 mg · g~(-1),粗蛋白含量5.16 mg · g~(-1),每667 m~2产量4000～4500 kg,适合广东节瓜产区秋季种植。     

苦瓜新品种‘俏绿珍珠苦瓜’

苦瓜新品种‘俏绿珍珠苦瓜’是以雌性较强的自交系LCP90-11为母本,早熟优质丰产油瓜自交系M5-2为父本杂交选育而成。第1朵雌花着生节位春季16.3节、秋季16.5节,第1坐瓜节位春季17.8节、秋季18.0节。瓜长圆锥形,瓜皮绿色,圆瘤为主,间有条瘤。瓜长27.9～30.6 cm,横径5.64～6.34 cm,肉厚1.11～1.12 cm。单瓜质量355.0～442.3 g,单株产量1.36～1.99 kg,一般栽培产量约37 500 kg·hm^-2。适宜广东、广西、海南和福建等地春、秋季种植。     

小南瓜新品种旭日的选育

旭日是由两个株系C2-8-1-9-4-3和B5-3-6-2-6-1配制而成的一代杂种,抗霜霉病、灰霉病和疫病,较抗枯萎病,耐病毒病和白粉病.植株长势中等,春季第一雌花节位为第7节,老熟瓜在开花后春季45～50天,秋季50～60天采收.单瓜重1.0～1.5 kg,瓜圆形稍扁,瓜皮瓜肉呈橙红色,品质甜粉.     

有棱丝瓜新品种雅绿8号的选育

雅绿8号是由优良强雌性自交系雅岗/三叶早丝瓜//F224与优质抗病自交系雅一F301配制而成的优质有棱丝瓜一代杂种,第1雌花着生节位春季为第6.7节、秋季为第12.3节,第1坐果节位春季为第9.1节、秋季为第14.1节。瓜呈长棍棒形,瓜色深绿,瓜外皮无花斑,棱沟深,棱色墨绿,瓜长47.9~51.8 cm,横径4.80~5.02 cm。单瓜质量379.4~458.0 g,单株产量1.24~1.58 kg,一般产量1 800~2 200 kg·(667 m2)-1。适宜华南地区春、秋季种植。     

茄子新品种紫荣9号的选育

紫荣9号是紫茄杂交一代新品种.从播种至始收春季105 d、秋季87 d,延续采收期春季42 d、秋季48 d,全生育期春季147 d、秋季135 d;门茄坐果率89.1％～89.2％;果长棒形,果上萼片呈紫绿色;果皮紫红色,果面光滑、光泽度佳、着色均匀;果长32.1～32.3 cm,果粗4.6～4.8 cm,单果质量2...     

复混生物有机肥在节瓜栽培上的应用试验

试验通过HUM微生物复合肥加钙镁磷肥作对比.在基本一致的土壤水平、管理水平下,研究毛节瓜的物候期、生长势、开花结果习性,以及产量等性状.结果证明:定植26 d后,施用HUM微生物复混肥的毛节瓜植株主蔓叶片数平均比对照多2.4张,主蔓长度平均比对照高37.68 cm;第1雌、雄花在主蔓上的着生节位降低1～3个节位,花期提前2～5 d;产量增加12.47%到45.62%,增产效果极显著.因此,施用HUM微生物复混肥比施用普通复合肥加钙镁磷肥效果好,以施用150 kg/667m2为最佳.     

Effects of radiation from 188Re on smooth muscle cell proliferation

AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β^-particles emission from ¹⁸⁸Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by ¹⁸⁸Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [³H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of ¹⁸⁸Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from ¹⁸⁸ Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G₀/G₁ arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation.     

Effect of L-Arg on plasma content of endothelin and expression of c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy

AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P＜0.01). The ET content in plasma also decreased significantly by L-Arg(P＜0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration.     

多效唑对猕猴桃离体试管苗生长及内源激素的影响

多效唑（ＰＰ３３３）处理猕猴桃试管苗，降低了其生长强度；植株体内的ＧＡ３、ＩＡＡ和ＺＴ含量下降，ＡＢＡ的含量上升，乙烯释放率增加；并且能降低外源的ＧＡ３和ＩＡＡ促进生长的作用，而外源的ＧＡ３和ＩＡＡ又能不同程度地逆转多效唑的抑制作用，使植株恢复生长。     

Proteomic analysis between pathological scars and normal skin

AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars.     

Compositional and Functional Properties of Saskatoon Berry and Blueberry

Abstract

Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared.     

Cryopreservation of shoot apices of hawthorn in vitro cultures originating from East Asia

The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species.     

Coupling past management practice and historic landscape change on John F. Kennedy Space Center,Florida

Historic landcover dynamics in a scrubby flatwoods (Tel-4) and scrub landscape (Happy Creek) on John F. Kennedy Space Center were measured using aerial images from 1943, 1951, 1958, 1969, 1979, and 1989. Landcover categories were mapped, digitized, geometrically registered, and overlaid in ARC/INFO. Both study sites have been influenced by various land use histories, including periods of range management, fire suppression, and fire management. Several analyses were performed to help understand the effects of past land management on the amount and spatial distribution of landcover within the study sites. A chi-squared analysis showed a significant difference between the frequency of landcover occurrence and management period. Markov chain models were used to project observed changes over a 100-year period; these showed current management practices being effective at Tel-4 (restoring historic landscape structure) and much less effective at Happy Creek. Documenting impacts of past management regimes on landcover has provided important insight into current landscape composition and will provide the basis for improving land management on Kennedy Space Center and elsewhere.     

Metallothionein inhibits homocysteine-induced proliferation of rat vascular smooth muscle cells

AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [³-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10^-6-10^-4 mmol/L) stimulated [³-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [³-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10^-6-10^-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl₂ for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation.     

XBP-01 accelerated apoptosis induced by oxidized low density lipoprotein in vascular smooth muscle cells

AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 h after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 h in vitro. The samples were analyzed by fluorescence microscope, confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis.     

The effect of exposure on landscape scale soil surface temperatures and species distribution models

Species distribution models (SDMs) often use elevation as a surrogate for temperature or utilise elevation sensitive interpolations from weather stations. These methods may be unsuitable at the landscape scale, especially where there are sparse weather stations, dramatic variations in exposure or low elevational ranges. The goal of this study was to determine whether radiation, moisture or a novel estimate of exposure could improve temperature estimates and SDMs for vegetation on the Illawarra Escarpment, near Sydney, Australia. Forty temperature sensors were placed on the soil surface of an approximately 12,000 ha study site between November 2004 and August 2006. Linear regression was used to determine the relationship with environmental factors. Elevation was correlated more with moderate temperatures (winter maximums, summer minimums, spring and autumn averages) than extreme temperatures (summer maximums, winter minimums). The correlation (r ²) between temperature and environmental factors was improved by up to 0.38 by incorporating exposure, moisture and radiation in the regressions. Summer maximums and winter minimums were predominately determined by exposure to the NW and coastal influences respectively, while exposure to the NE and SW was important during other seasons. These directions correspond with the winds that are most influential in the study area. The improved temperature estimates were used in Generalised Additive Models for 37 plant species. The deviance explained by most models was increased relative to elevation, especially for moist rainforest species. It was concluded that improving the accuracy of seasonal temperature estimates could improve our ability to explain the patchy distribution of many species. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.