PEG胁迫对萌动苦豆子子叶抗旱生理及氧化苦参碱含量的影响

以PEG-6000模拟干旱条件,测定苦豆子种子萌发、萌动种子子叶中质膜相对透性(PMP)、丙二醛(MDA)和脯氨酸(Pro)含量以及抗氧化物酶POD、CAT、SOD酶活性以及氧化苦参碱含量,探讨苦豆子种子的抗旱适应性。结果表明:10%PEG胁迫下,种子的发芽势、发芽率、发芽指数和活力指数均达到最高,依次为66.67%,78.67%,24.40和183.79,20%PEG胁迫种子仍有较高的发芽能力。随PEG胁迫程度加剧,萌动苦豆子种子子叶中质膜相对透性和Pro含量逐渐上升,MDA含量逐渐下降;POD、CAT和SOD 3种保护酶对干旱胁迫的保护作用不尽相同,中轻度胁迫(PEG≤20%)时POD酶起主要作用,重度胁迫(PEG20%)时CAT和SOD酶起主要作用。PEG胁迫使子叶中OMA含量下降,OMA含量与抗旱生理指标间均存在负相关。适度干旱有助于苦豆子种子萌发,萌动种子对干旱有强的防御机制。     

调亏灌溉对番茄生理指标、产量品质及水分生产效率的影响

在大棚条件下进行了番茄调亏灌溉试验研究.共设有T1,T2,T3,T4,T5五个处理,其中T1,T2分别在开花座果期做亏缺处理,田间持水量分别为55%～65%,45%～55%;T3,T4分别在果实膨大期做亏缺处理,田间持水量分别为65%～75%,55%～65%;T5为常规灌溉,两个时期的土壤含水量分别为65%～75%,75%～85%.结果表明,水分亏缺显著抑制番茄体内蛋白合成,根活力下降;脯氨酸、丙二醛含量有不同程度的上升.开花座果期以处理T1、果实膨大期以处理T3为最优设计.处理T1的总产量比对照T5提高3.8%,T3比T5减少5.3%;T1和T3的单果重分别比T5减少了5.8%、16.9%,而水分利用效率分别提高了19.9%、32.6%.T1、T3两个处理的果实品质明显改善:可溶性总糖、Vc和有机酸含量均与T5差异显著.处理T1的前期产量比T5增加了3.8%,说明复水后有补偿效应,有利于向果实运转与分配,促进生殖生长.     

4种处理对龙葵种子萌发的影响

以龙葵种子为材料,研究了微波、超声波、酸度和Al3＋对龙葵种子萌发的影响。结果表明,在适宜的条件下,450 W微波处理20 s、超声波处理15 min、pH值5．0～6．5最利于龙葵种子萌发;Al3＋处理能降低龙葵种子的发芽率、发芽势和发芽指数。     

玉米‖马铃薯抗旱栽培土壤含水量及产量效益研究

于2014年在宣威市农业技术示范基地开展了玉米间作马铃薯集雨保墒抗旱栽培复合技术试验研究。试验处理包括:T_1:玉米+开沟覆膜‖马铃薯;T_2:玉米+打塘覆膜‖马铃薯;T_3:玉米+平作覆膜‖马铃薯;T_4:玉米平作覆膜单作;T_5:马铃薯单作。结果表明,T_1和T_2处理玉米土壤含水量均高于其它处理,T_1、T_2处理0~7.6 cm土层玉米平均土壤含水量比T_4处理最多增加28.32%,0~20 cm土层最多增加28.12%;间作处理马铃薯的土壤含水量均高于马铃薯单作,T_1、T_2处理马铃薯平均土壤含水量在0~7.6 cm和0~20 cm两个土层分别比T_5处理最多增加23.52%和12.78%;T_1、T_2处理玉米产量、复合产量和土地当量比(LER)均高于T_3处理,其中,T_2处理的土地当量比最大(LER=1.20),比T_3处理增加10.52%,产量效益明显。     

不同耕作措施对旱地玉米生长环境及产量的影响

采用田间定位试验,研究了羊粪4 m3+深耕30~35 cm(MP3)、羊粪4 m3+深耕20~25 cm(MP2)、羊粪4 m3+旋耕10~15 cm(MP1)、深耕30~35 cm(P3)、深耕20~25 cm(P2)、旋耕10~15 cm(P1)6种耕作措施下旱地玉米土壤温度、土壤水分、土壤呼吸速率及产量的变化。结果表明:采用不同耕作措施均能有效促进旱地玉米生长,与传统耕作方式(P1)相比,MP3、P3处理的土壤体积含水量分别提高了39.00%、32.44%;MP3处理下土壤温度显著高于传统耕作0.94℃;随着旱地玉米生育期的推进,土壤呼吸速率峰值出现在6月底,不同耕作方式下土壤呼吸速率较传统耕作提高了0.68~1.26倍;深耕处理、深耕+羊粪处理的旱地玉米产量均显著高于传统耕作,其增幅在11.94%~36.51%。通过3年试验比较,羊粪4 m3+深耕30~35 cm、羊粪4 m3+深耕20~25 cm、深耕30~35 cm效果较好。     

不同化学试剂及人工处理对稗草种子休眠的影响

分别应用不同化学试剂及人工剥去颖壳处理来提高稗草种子的萌发率,不同处理对种子发芽率的影响不同.GA3、浓H2 SO4、KNO3、剥去颖壳处理都有利于稗草种子的萌发,但剥去颖壳破除休眠的效果不理想.通过800～1200 mg/L GA3浸种24 h,浓硫酸浸种10～ 20 min,都能打破稗草种子休眠,使其发芽率超过70％;KNO3溶液可打破部分稗草种子休眠,2％ KNO3浸泡12 h,发芽率为34.67％;NaOH、HC1不宜用于解除稗草种子休眠.     

马铃薯抗旱栽培土壤含水量及产量效益研究

于2014年在宣威市农业技术示范基地开展了玉米间作马铃薯集雨保墒抗旱栽培复合技术试验研究。试验处理包括：T₁：玉米+开沟覆膜‖马铃薯;T₂：玉米+打塘覆膜‖马铃薯;T₃：玉米+平作覆膜‖马铃薯;T₄：玉米平作覆膜单作;T₅：马铃薯单作。结果表明,T₁和T₂处理玉米土壤含水量均高于其它处理,T₁、T₂处理0～7.6 cm土层玉米平均土壤含水量比T₄处理最多增加28.32%,0～20 cm土层最多增加28.12%;间作处理马铃薯的土壤含水量均高于马铃薯单作,T₁、T₂处理马铃薯平均土壤含水量在0～7.6 cm和0～20 cm两个土层分别比T₅处理最多增加23.52%和12.78%;T₁、T₂处理玉米产量、复合产量和土地当量比（LER）均高于T₃处理,其中,T₂处理的土地当量比最大（LER=1.20）,比T₃处理增加10.52%,产量效益明显。     

引流式双扣漏斗加速排盐并抑制返盐试验研究

为有效解决盐碱地植物根系因返盐受到胁迫以及重度退化盐碱地修复难题,利用盐碱土地"盐随水动"典型特点,从淋盐和抑盐角度开展盐碱化土壤的微域改良研究,设计了可快速淋盐的引流式双扣漏斗型防盐害保水的植物栽培系统。对不同处理措施(单形漏斗试验、双扣漏斗试验、引流式双扣漏斗试验、引流式双扣漏斗加壤内隔层试验)效果进行试验研究。结果表明:(1)采用引流式双扣漏斗加壤内隔层(处理4)降盐效果最为显著。0~20 cm土层处理4较对照组土壤含盐量自3.64%下降至0.66%,降低达5.53倍。(2)采用处理4,棉花发芽、成苗率显著提升,发芽率由对照组40.0%提升至86.0%;成苗率较对照组0%提升至82.0%。(3)不同处理措施下土壤含盐量及棉花种子发芽、成苗率对比发现,采用引流式双扣漏斗加壤内隔层可快速降低引流式双扣漏斗境内土壤盐分,显著提高棉花成苗率。研究表明,该系统可高效淋洗引流式双扣漏斗区土壤盐分,降低区内水分蒸发,阻隔区外水盐上行,防止区内土壤盐化,创造出保护根系、适宜植物生长的"小生境"。     

叶甜菜种子发芽特性的研究

研究了不同化学药剂和植物激素浸种处理及种球色泽、粒径、储藏时间等对叶甜菜种子发芽的影响。研究表明,用一定浓度的双氧水、硼酸、磷酸二氢钾浸种处理叶甜菜种子,可提高种子发芽率、发芽势与发芽指数,其中以10g/kgH2O2、0.5g/kgH3BO3和5g/kgKH2PO4处理效果最好。赤霉素处理(10、30、50、70、100mg/L)叶甜菜种子,促进种子发芽的效果不明显。吸湿-回干处理对叶甜菜种子发芽有良好的促进作用。叶甜菜种球色泽、粒径大小与发芽的关系密切,黄色或黄褐色的种球比黑色或褐色种球及大、中粒种子比小粒种子发芽力显著;叶甜菜种子不耐贮藏,发芽力下降较快,使用年限仅为1a。     

西安地区丰水年有林地土壤水分恢复效应研究

通过对西安南郊平水年与丰水年秋季有林地土壤含水量的测定,研究了地下0～6 m之间土壤含水量的变化与土壤水分恢复问题.研究结果表明,正常年西安地区人工林2～4 m土壤含水量为9%～10%,发育了明显的土壤干层,表明土壤干层在黄土高原分布具有普遍性;丰水年西安南郊人工林下2～4 m的土壤含水量在18%～24%之间,高出正常年份这一层位含水量的8%～14%,土壤干层已经消失;丰水年4～6 m的土壤含水量也明显升高.分析得出,在降水丰富的年份,土壤水分得到恢复,而且恢复的深度可达5 m,恢复的速度也较快.     

Dermestid beetles in ‘evolution Canyon’, lower Nahal Oren, Mt. Carmel, including new records for Israel

Fifteen species of dermestid beetles were recorded at ‘Evolution Canyon’ (EC), Lower Nahal Oren, Mt. Carmel, Israel. They represent ~35% of known Israeli dermestid species. The following three species were recorded for the first time in Israel:Trogoderma svriaca Dalla Torre, 1911;Ctesias svriaca Ganglbauer, 1904; andAnthrenus (s.str.) jordaniens Pic, 1934. Adults of 13 species were collected on the more solar radiated, warmer and climatically more fluctuating south-facing slope (SFS); ten species were collected on the opposite, north-facing slope (NFS), which was cooler and climatically more stable. The abundance of adult dermestid beetles was 1.9 times higher on the SFS than on the NFS (86 and 47, respectively). Species richness and abundance distribution at EC (three collecting stations on each slope and one at the valley bottom) were significantly negatively correlated with the plant cover that consisted of trees and bushes (Spearmanr _s ,P=0.007 and 0.039, respectively) and perennials (Spearmanr _s ,P=0.039 and 0.077, respectively), indicating that non-woody plants were preferred by adult dermestid beetles.     

Epidemiology of Toxigenic Fungi and their Associated Mycotoxins for Some Mediterranean Crops

Recent data on the epidemiology of the common mycotoxigenic species of Fusarium, Alternaria, Aspergillus and Penicillium in infected or colonized plants, and in stored or processed plant products from the Mediterranean area are reviewed. Emphasis is placed on the toxigenicity of the causal fungal species and the natural occurrence of well known mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes, zearalenone, patulin, Alternaria-toxins and moniliformin), as well as some more recently described compounds (fusaproliferin, beauvericin) whose toxigenic potential is not yet well understood. Several Fusarium species reported from throughout the Mediterranean area are responsible of the formation of mycotoxins in infected plants and in plant products, including: Fusarium graminearum, F. culmorum, F. cerealis, F. avenaceum, F. sporotrichioides and F. poae, which produce deoxynivalenol, nivalenol, fusarenone, zearalenone, moniliformin, and T-2 toxin derivatives in wheat and other small grains affected by head blight or scab, and in maize affected by red ear rot. Moreover, strains of F. verticillioides, F. proliferatum, and F. subglutinans, that form fumonisins, beauvericin, fusaproliferin, and moniliformin, are commonly associated with maize affected by ear rot. Fumonisins, were also associated with Fusarium crown and root rot of asparagus and Fusarium endosepsis of figs, caused primarily by F. proliferatum. Toxigenic A. alternata strains and associated tenuazonic acid and alternariols were commonly found in black mould of tomato, black rot of olive and citrus, black point of small cereals, and black mould of several vegetables. Toxigenic strains of A. carbonarius and ochratoxin A were often found associated with black rot of grapes, whereas toxigenic strains of A. flavus and/or P. verrucosum, forming aflatoxins and ochratoxin A, respectively, were found in moulded plant products from small cereals, peanuts, figs, pea, oilseed rape, sunflower seeds, sesame seeds, pistachios, and almonds. Finally, toxigenic strains of P. expansum and patulin were frequently found in apple, pear and other fresh fruits affected by blue mould rot, as well as in derived juices and jams.     

A survey of the genera of<Emphasis Type="Italic">Microgaster</Emphasis> latreille and<Emphasis Type="Italic">Hygroplitis</Emphasis> thomson (Hymenoptera: Braconidae: Microgastrinae) from China

The genera ofMicrogaster Latreille 1804 andHygroplitis Thomson 1895 from China are presented systematically in this paper. Thirty-two species ofMicrogaster and three species ofHygroplitis are known in China. Diagnosis, character variation, distribution and host of each species among the two genera are presented, including its host and distribution. Keys to the species ofMicrogaster andHygroplitis are given. http://www.phytoparasitica.org posting Dec. 19, 2006.     

Plant Viruses Transmitted by Whiteflies

One-hundred and fourteen virus species are transmitted by whiteflies (family Aleyrodidae). Bemisia tabaci transmits 111 of these species while Trialeurodes vaporariorum and T. abutilonia transmit three species each. B. tabaci and T. vaporariorum are present in the European–Mediterranean region, though the former is restricted in its distribution. Of the whitefly-transmitted virus species, 90% belong to the Begomovirus genus, 6% to the Crinivirus genus and the remaining 4% are in the Closterovirus, Ipomovirus or Carlavirus genera. Other named, whitefly-transmitted viruses that have not yet been ranked as species are also documented. The names, abbreviations and synonyms of the whitefly-transmitted viruses are presented in tabulated form together with details of their whitefly vectors, natural hosts and distribution. Entries are also annotated with references. Whitefly-transmitted viruses affecting plants in the European–Mediterranean region have been highlighted in the text.     

Broad bean mottle virus in Morocco; curculionid vectors,and natural occurrence in food legumes other than faba bean (Vicia faba)

Broad bean mottle virus (BBMV) was transmitted from infected to healthy faba-bean plants by the curculionid weevilsApion radiolus Kirby,Hypera variabilis Herbst,Pachytychius strumarius Gyll,Smicronyx cyaneus Gyll, andSitona lineatus L. The latter appeared to be an efficient vector: acquisition and inoculation occurred at the first bite, the rate of transmission was c. 41%, and virus retention lasted for at least seven days.S. lineatus transmitted the virus from faba bean to lentil and pea, but not to the three genotypes of chickpea tested. This is the first report on the generaHypera, Pachytychius, andSmicronyx as virus vectors, and onA. radiolus, H. variabilis, P. strumarius, andS. cyaneus as vectors of BBMV.Out of 351 samples of food legumes with symptoms suggestive of virus infection, 16, 11, 19, and 17% of the samples of chickpea, lentil, pea, and common bean, respectively, were found infected when tested for BBMV in DAS-ELISA. This is the first report on the natural occurrence of BBMV in chickpea, lentil, pea, and common bean. The virus should be regarded as a food-legume virus rather than a faba-bean virus solely, and is considered an actual threat to food legume improvement programmes.     

Characterization of potato potyvirus Y (PVY) isolates from seed potato batches. Situation of the NTN, Wilga and Z isolates

A collection of 38 PVY isolates from seed potato batches, originating from several Western European countries, was characterized by using current biological, serological and molecular tools differentiating PVY strains and groups. The correlation between the three kinds of tests was good but not absolute. No single serological or PCR method was able to discriminate among the five isolate groups found. Twenty-nine isolates belonged to the PVY^N strain and six to the PVY^O strain. No PVY^C was found. Two other isolates reacted serologically like PVY^O, but were unable to elicit a hypersensitive response from the Ny_tbr gene and probably represent the PVY^Z group. At the molecular level, these two isolates showed a combination of both PVY^O and PVY^N and could be recombinants of these strains. Another isolate reacted serologically like PVY^O, but induced vein necrosis in tobacco, like PVY^N-Wilga. Some PVY^N isolates caused tuber ring necrosis in glasshouse conditions. These might belong to the PVY^NTN group. The PVY^NTN, PVY^N-Wilga and PVY^Z groups probably represent pathotypes within strains PVY^N and PVY^O, respectively. The present study also confirms previous reports showing a high genetic variation at the 5 end within the PVYN strain.     

The autumn leafroller: Phenology,damage and parasitoids in a Dutch apple orchard

The phenology of the autumn leafroller,Syndemis musculana, a local pest of apple, was studied in order to forecast larval emergence. From 1983–1986, peak flight as determined with sexpheromone traps was always between 13–18 May. The duration of embryonic development was determined at various constant temperatures and used to estimate the periods of egg hatch in these four years. Each year, most eggs should have hatched in the second decade of June.Differences in attack rates between apple cultivars seem to be explained largely by the variation in picking time. Larvae are only half grown at the beginning of harvest (cv. James Grieve), and have gone into hibernation when the latest variety (cv. Golden Delicious) is picked. Moreover, the varieties Cox's Orange Pippin and Belle de Boskoop, picked about half time, are liable to receive additional damage by caterpillars brought with the picked fruits into storage.Various hymenopterous parasites were reared from caterpillars. As the only leafroller in the orchard which hibernates as mature larva,S. musculana may promote winter survival of some parasitoids, like the eulophidColpyclypeus florus.Samenvatting De fenologie van de herfstbladroller (Syndemis musculana Hübner), een incidentele plaag op appel, werd nader bepaald met het doel het uitkomen van de eieren te kunnen voorspellen. In 1983–1986 viel de piekvlucht, bepaald met behulp van feromoonvallen, steeds tussen 13 en 18 mei.De ontwikkelingsduur van de eieren bij verschillende constante temperaturen werd gebruikt om de periode van uitkomen te schatten. De meeste eieren zullen ieder jaar in de eerste helft van juni uitkomen.Geconstateerde verschillen in schade tussen appelrassen blijken goeddeels terug te voeren op verschillen in pluktijdstip. De rupsen van de herfstbladroller zijn pas half-was als de eerste appels eind augustus geplukt worden, terwijl tegen het einde van de oogst begin oktober de meeste al in winterslaap zijn. Met name tussentijdse rassen, als Cox's Orange Pippin and Schone van Boskoop, lopen extra schade op doordat grotere rupsen met de geplukte vruchten in de kist terecht komen.Uit de rupsen werden negen, al van andere boomgaardbladrollers bekende, sluipwespen gekweekt, Omdat deze bladrollersoort, als enige in de boomgaard, als volgroeide rups overwintert, lijkt zij bij uitstek geschikt als winterwaard.This study was carried out at the Experimental Orchard De Schuilenburg, Schuilenburg 3, 4041 BK Kesteren, the Netherlands, to which address correspondence should be addressed.     

Characterization of Sclerotinia minor populations in Texas peanut fields

In the summer of 2004 an epidemic of sclerotinia blight of peanut, a disease caused by Sclerotinia minor, occurred in Texas in fields where the disease was never previously detected. The disease was observed on many plants within one of the fields (>3000 disease foci), although most foci were <1 m. It is hypothesized that these observations were inconsistent with the recent introduction of a monocyclic pathogen, even if disease developed under conducive environmental conditions. The pattern of disease is most suggestive of the presence of foliar (ascospore) infections, although air temperature was above the known limits for apothecia development if the pathogen had arrived in the field in 2004 peanut seed. To further examine this epidemic, 232 isolates were collected, across a variety of spatial scales spanning this field and other Texas peanut fields, and evaluated for aggressiveness, fungicide sensitivity and genotypic diversity. There was wide variation among isolates for the phenotypic characteristics measured, but there was no evidence that a genotypically unique, highly aggressive, and fungicide resistant isolate had been introduced or evolved. The predominant genotype, TX1, which contained 154 isolates, was found in every county and field population.     

Tumours of Begonia and some other ornamentals,induced by Corynebacterium fascians

In 1975 many tumours were observed in plants ofBegonia Schwabenland grown in Aalsmeer. Submersion of the roots ofNicotiana megalosiphon seedlings in a homogenate of tumorous tissue, induced tumours after two weeks. Short periods of submergence yielded results similar to those obtained after longer periods. Tumour homogenates lost their infectivity after ten min at 50°C. Aphids transmitted the infectious agent.Treatment with propylene oxide did not inhibit infectivity completely. Filtration through a 450 nm filter removed the infectious agent.Tobacco tumor virus or a viroid could not be isolated. Cultures ofCorynebacterium fascians, isolated from tumours ofN. megalosiphon were highly infectious and induced tumours in healthyN. megalosiphon andBegonia. Tumorous tissue homogenates ofPelargonium zonale, Dahlia sp.,Gladiolus sp., andLilium sp. also caused tumours inN. megalosiphon, from whichC. fascians was isolated. It was not possible to produce tumours inN. megalosiphon with homogenates from roses with symptoms of bud proliferation.Samenvatting In 1975 werden vele tumoren waargenomen inBegonia Schwabenland op Aalsmeerse bedrijven (Fig. 1). De infectiositeit van tumorweefsel kon goed en snel worden vastgesteld door de wortels van zaailingen vanNicotiana megalosiphon in een homogenaat van tumorweefsel te dompelen. Tumoren ontstonden na twee weken, de eindbeoordeling geschiedde na een maand (Fig. 2). Ook verschillende andereNicotiana spp.,Melilotus officinalis (Fig. 3) enPisum odoratum (Fig. 4) werden aangetast.Bij de infectiositeitstoets gaven zeer korte dompeltijden even goede resultaten als langere (Tabel 1). Infectieus sap verloor zijn infectievermogen na 10 min verhitting bij 50°C. Bladluizen brachten de smetstof over. Propyleenoxide verminderde de infectiositeit wel, doch onderdrukte deze niet totaal. Bij filtratie door een 450 nm filter bleef het infectieuse agens op het filter achter. Het tumor-inducerende agens was ook aanwezig in die delen van planten met tumoren welke gezond leken en het ging voor een gering deel over met zaad (Tabel 2).Uit tumoren konden wij geen tabakstumorvirus of een viroïde isoleren. Culturen vanCorynebacterium fascians, geïsoleerd uit tumoren vanN. megalosiphon bleken zeer infectieus en veroorzaakten tumoren inN. megalosiphon enBegonia. Homogenaten van tumorweefsel vanPelargonium zonale, dahlia (Fig. 5), gladiool (Fig. 6) enLilium Mid Century Hybrid Enchantment (Fig. 7) veroorzaakten ook tumoren opN. megalosiphon, waaruitC. fascians werd geïsoleerd. Met sap van kroeskopzieke rozen konden wijN. megalosiphon niet besmetten.     

Eyespot of Cereals Revisited: ITS phylogeny Reveals New Species Relationships

Four species so far classified in Pseudocercosporella or Ramulispora (hyphomycetes) are associated with eyespot disease symptoms of cereals. Two of these have been linked to teleomorphs that were described in Tapesia. Sequence data derived from the Internal Transcribed Spacer region (ITS1, 5.8S and ITS2) of the rDNA operon showed, however, that the eyespot fungi associated with Tapesia are not congeneric with Ramulispora sorghi, the type of Ramulispora. The genus name Tapesia is now rejected in favour of the conserved name Mollisia, which appears to comprise heterogeneous fungi. Tapesia yallundae is not closely related to the type of Mollisia, M. cinerea, but clusters separately, being more closely allied to species with Cadophora anamorphs. A new holomorph genus, Oculimacula, is therefore proposed for teleomorphs of the eyespot fungi, while the anamorphs are accommodated in Helgardia gen. nov.