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近年小反刍兽疫的传播呈扩大趋势,严重危害了畜牧业生产安全,成为山羊、绵羊以及野生小反刍动物的一种急性接触性传染病.因此,早期诊断是预防控制小反刍兽疫的重要手段,根据小反刍兽疫典型的临床症状可初步诊断,再通过实验室诊断技术的支持确诊小反刍兽疫.本文阐述了小反刍兽疫的流行病学特征和了小反刍兽疫病毒实验室诊断和防控技术,为该病的研究和有效防控提供依据. 相似文献
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对小反刍兽疫的流行情况、临床症状、病理变化、实验室诊断和防控措施进行了介绍,提出了小反刍兽疫的临床诊断要点和实验室诊断方法,旨在为有效保障养羊生产和羊肉市场供给,进一步做好小反刍兽疫防控工作提供参考。 相似文献
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近年来,我国小反刍兽疫疫情比较严重,造成了较大的经济损失.本文从小反刍兽疫流行病学、临床症状、诊断、防控措施进行综述,为有效防控小反刍兽疫提供理论支持. 相似文献
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主要从小反刍兽疫的病原学、流行病学、临床症状、病理变化、诊断等方面分析了小反刍兽疫的发病特征和诊断方法,从加强动物饲养管理、加强对群众的宣传教育以及加强动物卫生监督管理工作等6个方面介绍了小反刍兽疫的科学防控措施,以期为当前我国农村肉羊养殖户科学防控小反刍兽疫提供参考。 相似文献
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《中国畜牧兽医文摘》2016,(12)
本文阐述了新疆、甘肃等省发生小反刍兽疫疫情,小反刍兽疫的病原、临床症状、诊断要点等以及永靖县规模养羊场防控小反刍兽疫所采取的措施和对策,提出了防控建议。 相似文献
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《养殖与饲料.饲料世界》2018,(12)
小反刍兽疫是一种传播迅速、危害严重的病毒性传染病,做好小反刍兽疫的防控工作十分重要。本文主要论述了小反刍兽疫的流行特点、临床症状、鉴别诊断与预防措施,小反刍兽疫可以采取中西医结合的方式进行治疗,效果较好。 相似文献
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对小反刍兽疫的流行病学、临床症状、剖检变化及诊断要点进行了总结,并介绍了相关防控措施,以期为临床有效防控小反刍兽疫提供参考。 相似文献
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ZHAO Wen-nian WANG Qing-hua LIU Chun-ju WANG Shu-juan LEI Cheng-hong BAO Jing-yue WANG Zhi-liang 《中国畜牧兽医》2014,41(8):29-33
In order to obtain purified peste des petits ruminants virus F protein, the F gene of peste des petits ruminants virus strain Tibet 07 was cloned into transfer vector pFastBac/CT-TOPO, plasmid pFastBacCT-PPRV-F was then transformed into DH10Bac complement cells. The recombinant bacmid DNA was isolated and transfected into sf9 insect cells to express F protein. The expressed F protein was identified by using SDS-PAGE, Western blotting and indirect immunofluorescence assay. The recombinant F protein was successfully expressed in insect cells with a relative molecular of 59 ku.The sf9 cells infected by Bacmid-PPRV-F can reacted with positive serum of peste des petits ruminants.Using the Bac-to-Bac baculovirus expression system expressed the F protein of peste des petits ruminants virus strain Tibet 07.This work provides basis for development of rapid test for detection of peste des petits ruminants antibody. 相似文献
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小反刍兽疫研究现状 总被引:1,自引:0,他引:1
小反刍兽疫(Peste des petits ruminants,PPR)是由小反刍兽疫病毒(Peste des petits ruminants virus,PPRV)引起的一种主要感染小反刍动物的急性、接触性传染病,发病率、死亡率高.近年来,小反刍兽疫(PPR)呈扩散的趋势,成为重要的跨国动物传染病之一,我国周边国家频繁器发该病.2007年7月25日暴发于西藏自治区日土县的我国首例小反刍尊疫疫情,更是对我国如何做好该病的防控工作提出了新的要求和挑战.为了增强广大兽医工作者和相关人士对本病的认识,文中就小反刍兽疫的病原学、流行病学、临床症状、病理特征以及诊断方法等方面的研究现状进行了综述. 相似文献
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Four isolates of peste des petits ruminants virus were obtained from sick Nigerian sheep and goats. One was identical antigenically with the prototype Senegalese strain. A cross relationship was found between peste des petits ruminants virus and rinderpest virus based on neutralisation in vitro. 相似文献
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In 1990, a serosurvey on 1,474 small ruminants was conducted in four districts of Niger (Maradi, Zinder, Diffa and Dosso). The epidemiology and seroprevalence of eight diseases were studied: Brucella melitensis and B. ovis brucellosis, chlamydiosis, coxiellosis, contagious caprine pleuropneumonia, type A pasteurellosis, peste des petits ruminants, Rift Valley fever, Crimée-Congo haemorragic fever. The main health problem for the development of small ruminants farming, seems to be both pasteurellosis and peste des petits ruminants. 相似文献
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W P Taylor 《Research in veterinary science》1979,26(2):236-242
It was possible to distinguish separate serological responses when experimental goats were inoculated with either rinderpest virus or peste des petits ruminants virus. Examination of field samples established that peste des petits ruminants occur commonly in Nigerian sheep and goats although some villages have escaped recent infection. There was no evidence of infection with rinderpest in these animals. 相似文献
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目的将小反刍兽疫病毒M蛋白基因截短表达后用于特异性单抗制备及临床抗体水平检测。方法:用在线分析软件BepiPred分析小反刍兽疫病毒M蛋白潜在的B细胞线性表位,以本实验室构建的pCR2.1T-PPRV M质粒为模板,扩增三段截短的M基因,纯化后的PCR产物分别与克隆载体pCR2.1T连接,筛选出的阳性重组质粒经双酶切后,分别与表达载体pET-32a(+)及pGEX-6p-1连接,再将鉴定为阳性的重组质粒转化入E.coli BL21(DE3)菌株诱导表达,并用SDS-PAGE及Western blot验证。结果 PCR产物电泳,得到与预期大小相符的特异性片段。对连接克隆载体及表达载体的重组质粒双酶切后,均出现与预期一致的片段,DNA测序表明,插入片段序列与小反刍兽疫Nigeria75/1株M蛋白基因完全一致。重组蛋白经SDS-PAGE及Western blot鉴定,证明所构建的重组蛋白均获得高效表达,并均具有良好的反应原性。结论成功表达了小反刍兽疫截短M基因的蛋白,为制备特异性单抗及小反刍兽疫抗体检测奠定了一定基础。 相似文献
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A Bundza A Afshar T W Dukes D J Myers G C Dulac S A Becker 《Canadian journal of veterinary research》1988,52(1):46-52
In order to study the pathomorphology and immunohistochemistry of peste des petits ruminants, four goats and two sheep were inoculated intranasally with the Malig-Yemen strain of peste des petits ruminants virus. The animals developed fever, nasal discharge, oral erosions, cough and diarrhea. One goat and one sheep died and one moribund goat was killed. Three animals survived the infection. At necropsy, erosive stomatitis, pneumonia and gastroenteritis were found. Histopathologically the pneumonocytes and epithelial cells of the ileum had eosinophilic cytoplasmic and nuclear inclusions. By an indirect immunoperoxidase method, the nuclei and cytoplasm of the ileal epithelial cells of one goat contained positively (brown) stained antigen, which corresponded to viral nucleocapsids by electron microscopy. Virus appeared to be released through the microvilli of the epithelial cells. We also confirmed the formation of giant cells due to peste des petits ruminants virus. 相似文献