HPLC法测定仔猪止痢口服液中葛根素的含量

仔猪止痢口服液为葛根、藿香、板蓝根等中药组成的复方制剂，具有解肌清热、化湿止泻功能。主治仔猪黄白痢及其他原因引起的腹泻。该法以仔猪止痢口服液中的君药葛根所含主要活性成分葛根素为指标。采用HPLC法对其含量进行测定。经稳定性、精密度、重现性、回收率等实验研究表明，本法不仅简便易行，且灵敏度高，重现性好。     

连梅止痢口服液的薄层鉴别

目的:建立控制连梅止痢口服液的鉴别方法。方法:采用薄层色谱法对口服液中黄连、乌梅进行薄层色谱鉴别。结果:连梅止痢口服液中黄连、乌梅特征成分在与对照品色谱相应的位置上显现相同的荧光斑点,阴性对照品无干扰。结论:该薄层鉴别方法专属性强、重现性好,适用于连梅止痢口服液的质量标准控制。     

五根汤口服液质量标准研究

研究建立五根汤口服液的质控方法。采用薄层色谱法(TLC)对五根汤口服液中山豆根、板蓝根、葛根、红花、大黄进行定性鉴别;应用HPLC法测定制剂中葛根素的含量。结果表明,TLC斑点显色清楚,阴性样品无干扰;葛根素回归方程为y=4 667 560x+3 799.08 (r=0.999 9),线性范围为0.244 48～1.466 88μg,平均回收率为99.50%,RSD为0.38%(n=9)。说明针对本制剂所建立的质控方法操作简便,专属性强,准确度高,可有效控制五根汤口服液的质量。     

参金止痢口服液防治新生仔猪腹泻效果

正新生仔猪腹泻发生率高,病死率高,因其病因复杂,在防治工作中开发新型中兽药制剂已成为研究热点。参金止痢口服液是由党参、金银花、干姜、半夏、藿香、甘草、茯苓、陈皮、白术等经水醇提取法提取后,加工制成的,其具有芳香化湿、和胃止呕之功效,可用于脾胃不和与霍乱吐泻之证。参金止痢口服液防治新生仔猪腹泻的临床试验如下。1材料与方法1.1材料1.1.1试验动物某猪场同批次新生仔猪(随机     

3种止痢型中兽药液体制剂中非法添加黏菌素的HPLC-PDA检查方法的建立

为建立止痢型中兽药液体制剂中非法添加黏菌素的高效液相-二极管阵列色谱法,采用十八烷基键合硅胶为填充剂,流动相为乙腈∶硫酸钠磷酸水溶液(20∶80),硫酸钠磷酸水溶液∶硫酸钠水溶液(7 g/L)∶磷酸水溶液(6.8%)(50.5∶22.75)。检测波长为215 nm。结果显示,在该色谱条件下,3种止痢型中兽药液体制剂本底色谱峰对黏菌素E_1的测定无干扰;黏菌素E_1在0.04～2 mg/mL浓度范围内线性关系良好;杨树花口服液的检出限为10 mg/mL,板蓝根注射液及小檗碱注射液的检出限均为4 mg/mL;上述3种制剂的添加回收率在95.9%～97.9%之间,表明该方法回收率较好,准确度较高,可用于止痢型中兽药液体制剂中非法添加黏菌素的定性定量检测。     

仔猪痢清口服液对小鼠腹腔巨噬细胞吞噬功能的影响

仔猪痢清口服液是根据中兽医学理论和中医药理论在现代药学上应用的原理,用黄连、黄柏、白头翁等8味中药制成的纯中药复方制剂,经临床试用具有良好的防治效果.试验探讨了仔猪痢清口服液对小鼠腹腔巨噬细胞吞噬功能的影响,为研究仔猪痢清口服液的免疫增强作用和机理奠定了基础.     

参灵清瘟败毒口服液对小鼠免疫功能的影响

参芪清瘟败毒口服液是由清热解毒、补气固本、止咳平喘、通肾利尿等的中药组方制成的口服液。具有抗病毒、抗炎、提高机体免疫力等功能[1]。为探讨该口服液对机体的免疫作用机制,我们对服用本方后小鼠免疫器官(胸腺、脾脏)、腹腔吞噬细胞、淋巴细胞、免疫球蛋白以及补体的有关免疫指标进行了研究。1材料与方法1.1实验动物选择体重为(16±2)g小鼠,雌雄各半,由商丘医药高等专科学校实验中心提供。1.2药品与试剂参灵清瘟败毒口服液由商丘“天一”生物技术有限公司提供,批号20040401;黄芪多糖口服液,黑龙江生物制品一厂生产,批号20040801;RPMI…     

葛根芩连汤的实验研究与临床应用

葛根芩连汤出自张仲景《伤寒论》,由葛根、黄芩、黄连、甘草4味药组成,有解表清里、升清止痢之功,症见身热、下痢。近年来国内外医学家通过实验研究证明葛根芩连汤具有解热、抗菌、抗病毒、解痉、抑制胃肠运动、抗缺氧、抗心律失常、增强机体免疫功能等药理作用,现代临床常用于     

“炎必清”对种鸡增重提高产蛋量以及防病效果观察

“炎必清”是由金银花、连翅、黄芩、神曲、葛根、甘草等13种纯中草药制成,主要有解毒退热、抗菌消炎、凉血止痢、除湿、健脾养胃等功能。 一、试验目的 “炎必清”对种鸡防病、产蛋量、增重方面的效果观察。 二、药品来源 商丘地区古宋兽药厂生产的“炎必清”片,片重为0.2克。 三、试验场地 农科院鸡场。 四、试验方法 在鸡场内随机选择27只     

清热康口服液的薄层色谱鉴别研究

为了建立清热康口服液的薄层色谱鉴别的方法,给制定其质量控制标准提供依据。用薄层色谱法对清热康口服液处方中的穿心莲、板蓝根和大青叶进行定性鉴别。结果在薄层色谱中均能检出穿心莲、板蓝根和大青叶药味的特征斑点。表明本法操作简便可靠、专属性强、灵敏度高,可作为清热康口服液的定性鉴别和质量控制的方法。     

电感耦合等离子-质谱法测定饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅

应用电感耦合等离子-质谱技术(ICP-MS),建立饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅等元素的测定方法。对饲料样品的前处理方法、仪器工作参数和11种元素标准曲线进行优化;并以加标回收、分析方法比对和重复测试说明方法的准确性和精密性。方法在0～1 000 ng/ml范围内线性良好,仪器检出限为0.557 7～5.072 ng/ml,具有良好的精密度,其回收率在88.1%～104.4%之间,相对标准偏差小于5.0%。同时与原子吸收和原子荧光方法进行比对,测定结果相近。所建立的方法简单、快速,可替代原子吸收和原子荧光方法测定饲料中的11种金属元素,为饲料的质量控制提供理想的元素分析方法。     

Supplementation of broodmares with copper, zinc, iron, manganese, cobalt, iodine, and selenium

In experiment 1, 6 pregnant mares received a concentrate that contained a trace mineral premix that provided 14.3 mg Cu, 40 mg Zn, 28 mg Fe, 28 mg Mn, 0.08 mg Co, 0.16 mg I, and 0.16 mg Se/kg concentrate (group A). Seven mares received the same concentrate plus 502 mg Zn and 127 mg Cu once daily (group B). No differences (P > .05) in foal growth data, or Cu, Zn, and Fe concentrations of mare milk, mare serum, or foal serum were observed. In experiment 2, 6 pregnant mares received the same concentrate as group A (group C), and 8 mares received the same concentrate fortified with 4× the trace mineral premix (group D). Group C mares had higher serum Zn concentration at 1 day (P < 0.01) and 56 days (P < 0.04). Group C mares had higher milk Fe concentration at 28 days (P < .01), and group D mares had higher milk Cu concentration at 56 days (P < .01). Group C foals had higher serum Cu concentration at 14 days (P < .03). The results from this study provide no evidence to indicate that supplementing late gestating and lactating mares with higher dietary trace mineral levels than those recommended currently by NRC has any influence on foal growth and development, or on the Cu, Zn, and Fe concentrations of the mare milk, mare serum, or foal serum.     

Comparisons of Angus, Charolais, Galloway, Hereford, Longhorn, Nellore, Piedmontese, Salers, and Shorthorn breeds for weight, weight adjusted for condition score, height, and condition score of cows

Breed differences for weight (CW), height (CH), and condition score (CS) were estimated from records (n = 12,188) of 2- to 6-yr-old cows (n = 744) from Cycle IV of the U.S. Meat Animal Research Center's Germplasm Evaluation (GPE) Program. Cows were produced from mating Angus and Hereford dams to Angus, Hereford, Charolais, Shorthorn, Galloway, Longhorn, Nellore, Piedmontese, and Salers sires. Samples of Angus and Hereford sires were 1) reference sires born from 1962 through 1970 and 2) 1980s sires born in 1980 through 1987. The mixed model included cow age, season of measurement and their interactions, year of birth, pregnancy-lactation code (PL), and breedgroup as fixed effects for CW and CS. Analyses of weight adjusted for condition score included CS as a linear covariate. The model for CH excluded PL. Random effects were additive genetic and permanent environmental effects associated with the cow. Differences among breed groups were significant (P < 0.05) for all traits and were maintained through maturity with few interchanges in ranking. The order of F1 cows for weight was as follows: Charolais (506 to 635 kg for different ages), Shorthorn and Salers, reciprocal Hereford-Angus (HA) with 1980s sires, Nellore, HA with reference sires, Galloway, Piedmontese, and Longhorn (412 to 525 kg for different ages). Order for height was as follows: Nellore (136 to 140 cm), Charolais, Shorthorn, Salers, HA with 1980s sires, Piedmontese, Longhorn, Galloway and HA with reference sires (126 to 128 cm). Hereford and Angus cows with reference sires were generally lighter than those with 1980s sires. In general, breed differences for height followed those for weight except that F1 Nellore cows were tallest, which may in part be due to Bos taurus-Bos indicus heterosis for size.     

液相色谱-串联质谱法测定动物尿液中11种β-受体激动剂残留量的不确定度评估

采用液相色谱-串联质谱法测定动物尿液中11种β-受体激动剂残留量,对标准溶液、体积、质谱峰面积、浓缩过程及回收率等测定不确定度因素进行了分析,通过评定各不确定度分量及标准不确定度,得出11种β-受体激动剂的扩展不确定度在0.7 ～ 1.1 ng/mL范围内.由各因素对合成不确定度的贡献比分析可知,影响较大的因素为试验回收率及标准溶液浓度.     

Granulocyte isolation from whole blood of goat, sheep, cattle, horse, dog, pig, and man

A simple two step procedure for the isolation of caprine, ovine, bovine, equine, canine, porcine and human peripheral blood granulocytes is described. After enrichment of granulocytes by centrifugation, contaminating erythrocytes are lysed hypotonically. Recovery, purity, and viability of the granulocyte suspensions are determined. FACScan analysis of the cell suspensions measuring cellular size by forward and sideward light scatter is compared with the corresponding analysis of whole blood leukocytes. Constituencies of the isolated cell suspensions and loss of granulocyte subpopulations through isolation procedure is discussed with regard to granulocyte function assays.