Soil Micronutrients and Citrus Growth

By using nutritional diagnosis of citrus leaves and determining soil micronutrients,the relationship between soil micronutrients and citrus growth in southern China has been studied.Studies showed that there was a significant positive correlation between available micronutrients (such as Zn,Mo,Cu)in the soil and the corresponding nutrients in citrus leaves.Thus,one can roughly learn of the sufficiency or deficiency of certain nutrients in soils by analyzing citrus leaves.Rational spray of Zn B or Mo fertilizer not only improved citrus yields but also increased the total sugar of Satsuma mandarin and of Xinhui orange by 2.9 and 17.2% respectively compared with the control.Spraying Mo fertilizer increased the vitamin C content of Satsuma mandarin juice by 4.7%-8.4%,maturated fruits 7-10 days earlier and gave the peel a brighter color.The ultramicroscopic characteristics of Zn-deficient citrus leaves were investigated under an electron microscope.Results showed that the Zn-deficient leaf cell was characterized mainly by poor cytoplasm,endoplasmic reticula and ribosomes and by big starch grains in the chloroplast.As a result of spraying Zn fertilizer the structure of the cell returned to normal,the cytoplasm became rich and the amount of chloroplast increased.There also appeared a great deal of multiform endoplasmic reticula,thus promoting the photosynthesis of Zn-deficient plants.This provides a cytologico-theoretical basis for fertilization of high-yielding citrus trees.     

Combined Application of Boron and Zinc Influence the Leaf Mineral Status,Growth, Productivity and Fruit Quality of ‘Kinnow’ Mandarin (Citrus nobilis Lour × Citrus deliciosa Tenora)

The deficiency of mineral nutrients, particularly of boron (B) and zinc (Zn) in many citrus orchards of Pakistan affect yield and quality. Effect of foliar application of B and Zn on leaf mineral nutrients, growth, productivity, and quality of ‘Kinnow’ were studied. Trees were sprayed with 37 mM boric acid and 49 mM zinc sulfate either alone or in combination at fruit setting stage or fruit setting and pre-mature stages. Leaf phosphorus (P), potassium (K), B, and Zn were significantly increased with treatments. Lowest fruit drop and maximum fruit weight and number, highest level of ascorbic acid, and reducing sugars were recorded in the trees sprayed with combine application of B and Zn at fruit set stage. In conclusion, combined application of B and Zn at fruit setting stage can be used effectively to increase nutrient uptake, reduce fruit drop, and increase yield with better quality of ‘Kinnow’ mandarin fruit.     

Micronutrient Contents of Citrus Orchard Soils in Southern China

Investigations were carried out on the micronutrient contents,of major citrus orchard soils (involving seven soil great groups in 8 provinces and an autonomous region of southern China),and an evaluation on the abundance and deficiency of available micronutrients in these soils was made in this paper.In southern China,citrus orchard soils derived from sandstone,sandy shale,Quaternary red clay,diluvial deposit,granite gneiss and neritic deposit were deficient in available Mo and B and low in Zn.Those developed on purple sandy shale,limestone and slope deposit were all in short supply of available Zn,B and Mo.Coastal solonchak was fairly abundant in B,but its available Fe,Zn and Mo contents were rather low.     

Nutrient Indexing in “‘Kinnow” Mandarin (Citrus deliciosia Lour.× Citrus nobilis Tanaka) Grown in Indogangetic Plains

Diagnosis and remediation of nutrient constraints in perennial fruit crop like citrus are the two important pillars of an effective nutrient management program. Efforts were made to develop nutrient indexing (NI) criteria based on generated leaf and soil analysis dataset for “Kinnow” mandarin (Citrus deliciosia Lour. × Citrus nobilis Tanaka) grown on illitic soils of Indogangetic plains (Entisol, Inceptisol, and Aridisol). NI through diagnosis and recommendation integrated system (DRIS) using leaf analysis data showed optimum value of leaf nutrient concentration as 2.22–2.32% nitrogen (N), 0.11–0.15% phosphorus (P), 1.10–1.41% potassium (K), 2.32–2.79% calcium (Ca), 0.38–0.61% magnesium (Mg), 22.4–58.3 ppm iron (Fe), 26.3–56.2 ppm manganese (Mn), 4.2–7.2 ppm copper (Cu), and 21.3–26.9 ppm zinc (Zn) vis-à-vis a fruit yield of 32.4–56.1 kg tree^?1. Using these NI criteria, Zn was observed as most deficient (64.7%) followed by Fe (61.5%), Mn (57.6%), N (96.1%), and P (38.5%) using percentage of orchards as basis. While, optimum NI (mg kg^?1) using soil analysis data was determined as 114.3–121.2 potassium permanganate (KMnO₄-N), 7.8–12.3 Olsen-P, 96.4–131.3 ammonium acetate (NH₄OAc)-K, 189.4–248.6 NH₄OAc-Ca, 72.3–89.9 NH₄OAc-Mg, 5.8–11.1, DTPA-Fe, 4.3–6.9 diethylenetriaminepentaacetic acid (DTPA)-Mn, 0.45–0.69 DTPA-Cu, and 21.3–26.9 DTPA-Zn for the optimum yield of 32.4–56.1 kg tree^?1. Soil analysis-based NIs displayed a good complementary with leaf analysis-based NIs evident from the diagnoses indicating Mn (52.2%) as most dominant constraint Zn (61.2%) followed by Mn (48.3%), N (41.2%), and P (35.6%). The recommended DRIS-based NIs would lay a scientific basis in formulating citrus fertilization program.     

Citrus flavanones: what is their role in cardiovascular protection?

Flavanones, including hesperidin and naringin, are polyphenolic compounds highly and almost exclusively present in citrus. Epidemiological studies reported an inverse relationship between their intake and the risk of cardiovascular diseases. Clinical and experimental data further showed their antihypertensive, lipid-lowering, insulin-sensitizing, antioxidative, and anti-inflammatory properties, which could explain their antiatherogenic action in animal models. Although flavanones may be promising compounds that are particularly active in cardiovascular disease prevention, clinical data are still scarce and most in vitro data have been obtained under nonphysiologically relevant conditions. Moreover, the mechanisms responsible for flavanone action are not fully elucidated. Therefore, further research is needed to better evaluate and understand the protective effects of flavanones in cardiovascular diseases.     

柚(Citrus grandis Osbeck)第一染色体的显微分离

本研究利用显微操作技术分离木本果树植物柚单个染色体，以Guan溪蜜柚的20个良好的染色体分裂相为基础，建立Guan溪蜜柚染色体核型图，并以此为依据，识别第一染色体，红显微操作，将单条是一染色体放入Eppendorf管中，为木本植物小型染色体的微切割，微分离和微克隆奠定了基础。     

Spatial distribution of glomalin-related soil protein and its relationships with root mycorrhization,soil aggregates,carbohydrates, activity of protease and β-glucosidase in the rhizosphere of Citrus unshiu

Relationships between the spatial distributions of glomalin-related soil protein (GRSP) and soil aggregates, carbohydrates or relevant enzymes are poorly studied. We found that two categories of GRSP, the easily extractable Bradford-reactive soil protein (EE-BRSP) and total BRSP (T-BRSP), respectively ranged between 0.3–0.6 and 0.5–0.8 mg/g DW soil, and these two BRSPs decreased with the increase of soil depth (0–40 cm) in the rhizosphere of a 22-year-old Citrus unshiu orchard. Both EE-BRSP and T-BRSP were significantly positively correlated with mycorrhization, 0.25–0.50 mm soil water-stable aggregates, water-extractable or hydrolyzable carbohydrates, and β-glucosidase, but significantly negatively correlated with protease. Our results demonstrate that the spatial distribution of GRSP is significantly affected by mycorrhization, soil carbohydrate, β-glucosidase and protease.     

Soil nutrient status,yield and quality of lemon (Citrus limon Burm.) cv. ‘Assam lemon’ as influenced by bio-fertilizers,organics and inorganic fertilizers

An experiment was conducted at Mizoram University, Aizawl, India during 2015-2017 to study the effect of integrated nutrient management on yield and quality of lemon cv. ‘Assam lemon’ (Citrus limon Burm.). The experiment was comprised of nine treatments viz. T1: 100% recommended dose of fertilizers (RDF) through inorganic fertilizers (IF); T2: 100% N through Farm Yard Manure (FYM); T3: 75% N through FYM + 25% through IF; T4: 50% N through FYM + 50% through IF; T5:75% N through FYM +25% through IF + Azotobacter + phosphate solubilizing bacteria (PSB)+ potash solubilizing bacteria (KSB); T6: 50% N through FYM + 50% through IF+ Azotobacter + PSB?+?KSB; T7: 75% N through FYM + 25% through IF?+?Azospirillum + AMF?+?KSB; T8: 50% N through FYM + 50% through IF+ Azospirillum + arbuscular mychorrhizal fungi (AMF) + KSB; T9: control. Among all treatments, T5 recorded highest value with respect to growth, yield and soil quality. While, T7 showed superiority in quality characters.     

Mechanism of visceral fat reduction in Tsumura Suzuki obese, diabetes (TSOD) mice orally administered β-cryptoxanthin from Satsuma mandarin oranges (Citrus unshiu Marc)

The carotenoid β-cryptoxanthin (β-CRX) is abundant in Satsuma mandarins (Citrus unshiu Marc). Several studies have shown a relationship between Satsuma mandarin consumption and a low risk of several diseases, for example, diabetes, gout, and hypertension, suggesting β-CRX involvement in disease prevention. We investigated the effect of β-CRX on mildly obese males. β-CRX administration reduced visceral adipose tissue, body weight, and abdominal circumference. However, the detailed mechanism by which β-CRX mediates these changes remains unknown. To identify this mechanism, we used an obese model mouse (TSOD). Oral β-CRX administration repressed body weight, abdominal adipose tissue weight, and serum lipid concentrations in TSOD; these results are identical to previous human trial results. β-CRX administration significantly repressed adipocyte hypertrophy. Gene expression analysis strongly indicated that β-CRX can alter cytokine secretion and cell proliferation. These results suggest that β-CRX derived from Satsuma mandarins can help prevent obesity by repressing hypertrophy of abdominal adipocytes.     

航天搭载后福橘(Citrus reticulata Blanco)茎尖无性系有丝分裂行为和细胞超微结构的变化

福橘(Citrus reticulata Blanco)茎尖经‘实践八号'育种卫星搭栽后,经组织培养获得其再生植株,以此为材料,结合常规压片法与超薄切片技术,研究航天搭载对福橘当代无性系细胞有丝分裂行为及细胞超微结构的影响.结果表明:航天搭载对福橘茎尖具有诱变作用,所得13个诱变无性系中,有丝分裂过程存在不同程度的异常...     

Assessment of genetic variability in the endangered <Emphasis Type="Italic">Citrus macroptera</Emphasis> Mont. (“hatkora”) from Mizoram,north-east India

Assessment of genetic variability in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In view of this, 39 “hatkora” (Citrus macroptera Mont.) genotypes collected from different locations of Mizoram, India were evaluated for physico-chemical characteristics during 2013–2015. The study reveals that there was significant variation among the collections in these particular traits. Individual fruit weight ranged from 277.78 to 617.69 g; fruit diameter 9.32–12.52 cm; fruit length 7.74–10.19 cm, fruit volume 238.33–583.33 cc; pulp weight 153.75–320.94 g; pulp:peel ratio 1.50–3.24 and seed number 9.33–23.6. Similarly, the chemical parameters also varied significantly among different germplasms. The juice content varied from 13.45 to 32.53 %, ascorbic acid 34.81–73.64 mg/100 mL, TSS 6.15–9.10 %, acidity 5.03–8.75 %, total sugars 5.16–7.97 % and sugar:acid ratio 0.75–1.52. From the analysis of genetic parameters, it could be concluded that characters like fruit weight, fruit length, fruit diameter, fruit volume, pulp weight, pulp–peel ratio, juice, Total soluble solids (TSS), acidity, ascorbic acid, total sugars, and sugar–acid ratio could be used as selection criteria for development of effective and productive plant types in “hatkora”. Wide range of variation in physico-chemical parameters of “hatkora” fruits indicated the great scope of individual plant selection based on these characters for future genetic improvement programme.     

氮素形态, 光合作用, 光呼吸

Under high light conditions, ammonium nutrition has a negative effect on plant growth. This suggests that the adverse effects of ammonium nutrition on plant growth may be related to carbon gain, photosynthesis, and photorespiration. However, there is no consistent evidence of a specific mechanism that could explain the plant growth reduction under ammonium supply. It is generally accepted that during the light reaction, a surplus of nicotinamide adenine dinucleotide hydrogen phosphate （NADPH） is produced, which is not completely used during the assimilation of CO2, Nitrate reduc- tion in the leaf represents an additional sink for NADPH that is not available to ammonium-grown plants. Nitrate and ammonium nutrition may use different pathways for NADPH consumption, which leads to differences in photosynthesis and photorespiration. The morphological （i.e., cell size, mesophyll thickness, and chloroplast volume） and enzymic （i.e., ribulose-1,5-bisphosphate carboxylase/oxygenase （Rubisco）, phosphoenolpyruvate carboxylase （PEPCase）, and glutamine synthetase/glutamate synthetase （GS/GOGAT）） differences that develop when plants are treated with either nitrate or ammonium nitrogen forms are related to photosynthesis and photorespiration. The differences in photorespiration rate for plants treated with nitrate or ammonium are related to the conversion of citrate to 2-oxoglutarate （2-OG） and photorespiratory CO2 refixation.     

Release Rates of Ammonium‐Nitrogen,Nitrate‐Nitrogen,Phosphorus, Potassium,Magnesium, Iron,and Manganese from Seven Controlled‐Release Fertilizers

Abstract

Samples of seven controlled‐release fertilizers, Nutricote Total 13–13–13, Nutricote Total 18–6–8, Osmocote Plus 15–9–12, Osmocote 13–13–13, Polyon 18–6–12, Polyon 14–14–14, and Plantacote 14–8–15, were placed in leaching columns containing acid‐washed sand. Samples of all leachates were analyzed weekly to determine release rates of ammonium‐nitrogen (N), nitrate‐N, phosphorus (P), potassium (K), magnesium (Mg), manganese (Mn), and iron (Fe). Release rates for P from all products were slower than those for NH₄‐N, NO₃‐N, and K. Release of Mg, Mn, and Fe was very poor, with less than 50% of the total amount of each of these elements ever being released from the prills for some products. Nutricote products released Fe and Mn more effectively than did Osmocote or Plantacote.     

我国东部七省（闽,浙,赣,湘,鄂,苏,皖）生态系统对酸沉降的临界负 …

本文介绍了生态系统对酸沉隆的临界负荷的概念及一般确定过程。叙述了用ＭＡＧＩＣ区域数学模型求算临界负荷的方法，包括计算单元的选择、采样点的布设及样品的采集、土壤参数的确定、地表径流和地表水参数的研究以及临界负荷的计算方法。     

遥感,地图,地理信息系统一体化应用

遥感、地图和地理信息系统三者呈“你中有我，我中有你”的相辅相成关系。三者一体化应用使地球科学得以进展，又能在资料开发、环境保护、自然灾害监测评价等方面发挥重要作用。一体化应用的基础是掌握三者的学科－技术特性与相通关系。