叶绿素仪在夏玉米氮营养诊断中的应用

叶绿素仪(SPAD-502)的出现使通过无损伤的田间速测对作物进行氮营养诊断成为可能。采用简单的试验和研究方法在田间条件下研究了叶绿素仪应用于夏玉米氮营养诊断的可行性。研究结果表明,应用叶绿素仪监测夏玉米氮营养状况的最佳测定部位为最上部完全展开叶的中部,该部位叶绿素仪测定值与作物全氮、施氮量及产量之间均有较好的相关性。由于作物品种对叶绿素测定影响很大,需要通过相对叶绿素仪读数的方法对其进行校正,校正后叶绿素仪对夏玉米追肥推荐中营养状况的预测精度为66.7%。     

考虑植株氮垂直分布的夏玉米营养诊断敏感位点筛选

探明夏玉米氮素营养生化指标(叶绿素a、叶绿素b、类胡萝卜素、叶片氮含量和叶片氮积累量)与叶片SPAD值垂直分布特征及两者间定量回归关系,确立基于叶绿素仪的夏玉米氮营养无损诊断敏感叶位和叶片部位,以实现氮营养时空变化的快捷和精准监测。利用2018-2019年连续2季不同氮营养水平下夏玉米关键生育期主茎各叶位(顶1叶~顶12叶,TL1~TL12)和叶片部位(每张叶片从叶片基部开始根据叶片长度每20%分为1个测试区间) SPAD值及氮营养指标数据,研究基于偏最小二乘(partial least square, PLS)回归模型的夏玉米不同位点SPAD值与氮营养指标间关系,确定可稳定指示夏玉米氮营养空间异质性变化的敏感叶位及叶片部位。结果表明,不同叶位间夏玉米叶片SPAD值和氮营养指标于植株间分布均呈典型的"钟型"特征,至TL5或TL6时达至峰值。同一叶位不同部位间SPAD值由20%至100%位点时则逐步升高,且80%~100%位点间无显著差异(P>0.05)。PLS分析结果显示,夏玉米不同叶位SPAD值与氮营养指标间模型精度决定系数(coefficient of determination, R2)和相对分析误差(relative percent deviation,RPD)范围分别为0.693~0.821和1.425~2.744。不同测试位点R2和RPD值范围则分别为0.660~0.847和1.607~2.451,满足模型精确诊断需求。此后,基于PLS模型中各叶位和叶片部位无量纲评价指标变量重要性投影值(variable importance for projection,VIP),确定顶4叶(TL4)完展叶60%~80%区间为夏玉米氮营养诊断的敏感区域,VIP值均高于临界值1.40,预测效果较为理想。研究可为实现氮营养的高效、快捷诊断和精准施氮提供参考。     

叶绿素仪在氮肥推荐中的应用研究进展

叶绿素仪(Chlorophyll.meter)是近年来欧美一些国家在推荐施氮中开始使用的一种新型便携式仪器。这种仪器以叶绿素对红光和近红外光的不同吸收特性为原理来测定植物叶片的相对叶绿素含量,通过叶绿素与叶片全氮的关系来反映作物的氮营养状况,进而确定作物是否缺氮。这种新型仪器的使用为简便、快速、准确地进行氮肥推荐提供了一种新的思路。因此,通过研究不同作物,不同种植条件下叶绿素仪测定值与作物叶片全氮、作物产量之间的相关性,确定叶绿素仪测定值的临界水平,以及不同作物的测定部位、样品采集数量及影响测定准确性的因素,使这种技术尽快地应用于田间生产,有助于推动我国推荐施氮技术的进步。     

利用叶绿素计(SPAD-502)诊断水稻氮素营养和推荐追肥的研究进展

叶绿素计(SPAD-502)在监测水稻氮素营养水平和及时提供追肥所需的信息方面有快速、简便、无损的特点,但其精度常受多种因素的影响。本文分析了影响利用SPAD-502叶绿素计诊断水稻氮素营养和推荐追肥精度的水稻品种、生育时期、测定叶位、测定叶片的位点、生态环境等因素。并综述了通过量化SPAD读数与氮的关系,提高诊断水稻氮素营养精度;通过量化SPAD读数与追肥关系,提高SPAD-502叶绿素计诊断法推荐施肥精度的研究现状;提出进一步提高利用SPAD-502叶绿素计诊断水稻氮素营养状况与推荐追肥精度尚需解决的几个问题。     

Comparative Boron Nutritional Diagnosis for Olive Based on July and January Leaf Samplings

In this work, diagnosis of boron (B) nutritional status based on leaf B concentrations was compared for the most common leaf-sampling times for olive trees, January and July. For this purpose, field experiments were conducted over 4 years (2003–2006) in two rainfed olive groves located in Mirandela and Bragança, northeastern Portugal. Leaf samples were collected in January and July and analyzed for B by standard procedures. Fruit harvest occurred in December of each year. The crops followed typical alternate fruiting cycles. During the 4 years of the study, mean olive yields in the Bragança orchard fluctuated, yielding 3.6, 28.1, 5.5, and 22.7 kg tree^?1. Yield variation per individual tree was also great. In the Bragança orchard and in the 2004 harvest, yields ranged from 1.2 to 52.7 kg tree^?1. Leaf B concentrations also varied greatly between individual trees. In the Bragança orchard in the July sampling of 2004, values for individual trees varied from 12.2 and 23.7 mg B kg^?1. From a total of 16 scatterplots generated from the relationship between leaf B concentrations and olive yields, 10 significant linear relationships were established; six of them were related to July sampling dates and four were related to January dates. The number of significant linear relationships established between leaf B concentration and olive yield was used as a criterion of the accuracy of the B nutritional diagnosis, because this represented the lowest experimental variability. By using this criterion, the July sampling date proved as better for B nutritional diagnosis, although the difference from January sampling date was not sufficient to disregard this. Leaf B concentrations were consistently greater in July than in January. Averaged across the 4 years of the study in both orchards, the difference was 4.3 mg B kg^?1. This difference should be taken into account in the interpretation of leaf analysis results when B levels are close to the deficient critical concentration.     

Estimation of Leaf,Root, and Sap Nitrogen Status using the SPAD‐502 Chlorophyll Meter for Ornamental Shrubs

Abstract

The SPAD‐502 chlorophyll meter was evaluated as a rapid tool to predict plant nitrogen (N) concentration. The SPAD‐502 index utility, as a comparative tool with respect to N nutritional status, was evaluated for Laurustine (Viburnum tinus L.), tobir (Pittosporum tobira Thumb.), and strawberry tree (Arbutus unedo L.). The effect of sampling time on the SPAD‐502 index measures was also evaluated. Two sand culture experiments evaluating 1, 3, 5, and 7 mmol_c · L^?1 of total N concentration in nutrient solution and 70/30, 50/50, 40/60, and 30/70% nitrate/ammonium (NO₃ ^?/NH₄ ⁺) ratios were applied. In a media experiment, with similar conditions and fertilization, the accuracy of the SPAD‐502 regression data was evaluated. Leaf, root, and sap samples were taken in the middle and at the end of each experiment. Regression equations were established among leaf N, root N, of sap N analyzed concentrations and SPAD values with regard to global, as well as specific, data within each sampling time. Regression coefficients (b) among leaf N and SPAD were highly significant (P<0.05) for practically all sampling times. However, liner regression equations were different in all treatments among all the sampling dates. Determination coefficients (R²), in some cases, show that the SPAD‐502 index is not an adequate method for leaf, root, or sap N concentration fir Viburnum tinus L. and Pittosporum tobira Thumb. or for root or sap for Arbutus unedo L. in these experimental conditions. Nonetheless, the SPAD‐502 index could be a useful tool for relative comparison purposes and field operational nutrient management. However, the SPAD‐502 leaf greenness meter is a relatively good tool for leaf N and nutritional diagnosis for Arbutus unedo L. within the 38–66 SPAD‐502 index range.     

Effect of Soil Management on Olive Yield and Nutritional Status of Trees in Rainfed Orchards

Studies on the effect of groundcover treatments on perennial tree crops have been common in recent decades. However, few have included leaf analysis as an aid to understand the effects of groundcover treatments on tree crop growth and yield, in particular in rainfed olive orchards. Field experiments took place in northeast Portugal, over the course of eight consecutive years, in two commercial orchards selected on the basis of their contrasting situation regarding the floor-management system before the trial started. An orchard located in Bragança, currently managed as a sheep-walk, received the following treatments: sheep-walk (SW), where the natural vegetation was managed with a flock of sheep; mechanical cultivation (MC), which consisted of two tillage trips per year in the spring; and glyphosate (Gly), where the herbicide was applied once during the first fortnight of April. Another orchard near Mirandela, currently managed by tillage, received the following treatments: mechanical cultivation (MC); glyphosate (Gly); and residual herbicide (RH), where an herbicide with a residual component was applied late in the winter. The trees that underwent Gly treatments produced the greatest tree crop growth and olive yield. The worst results were achieved with the SW and MC treatments in the Bragança and Mirandela experiments, respectively. Leaf nitrogen (N) and boron (B) concentrations were significantly higher and lower, respectively, in the treatments that caused the higher and lower olive yields in both experiments. In the Mirandela orchard, where the leaf potassium (K) concentrations were close to the lower limit of the adequate range, the leaf K levels followed the pattern registered for N and B. The results showed a strong link between tree crop nutritional status and tree crop growth and olive yield. The groundcover treatments that facilitate nutrient absorption by olive trees yielded more crops.     

基于SPAD-502与GreenSeeker的冬小麦氮营养诊断研究

用GreenSeeker和SPAD-502测定了不同氮素处理的冬小麦冠层NDVI与叶片SPAD值, 分析了它们与叶片全氮、叶绿素含量及产量间的关系。结果表明: 冬小麦抽穗期SPAD值和NDVI值均与叶绿素含量呈极显著正相关; 除抽穗期和返青期外, SPAD值与叶氮含量、叶绿素含量的相关系数在其余各生育期均达到显著或极显著水平; NDVI值与叶氮含量、叶绿素含量在拔节期、乳熟期的相关性同SPAD值一致; SPAD值可以进行叶绿素的诊断, NDVI值可以进行氮的诊断。氮营养诊断时期应该选择拔节期。通过回归建立了基于SPAD值、NDVI值的产量估测模型, 可以通过SPAD值、NDVI值对冬小麦产量进行估算。     

Relationship between chlorophyll meter readings and nitrogen in poinsettia leaves

Chlorophyll meters are used for non-destructive estimation of leaf nitrogen (N). The objective of this study was to evaluate the readings with several chlorophyll meters (SPAD-502, atLEAF, and CCM-300), different sampling sites on leaves, number of leaves used for sampling, and different types of leaf N sampling on estimation of N in leaves of potted poinsettia (Euphorbia pulcherrima) “Prestige Red.” Results showed that all meters gave readings that were correlated for N determination and also were correlated with each other. SPAD and atLEAF showed interaction between different N treatments and different sampling sites on the leaves, while CCM readings were affected by different sampling sites on the leaf. atLEAF readings showed interaction between number of leaves sampled and different N treatments. Thus, during sensor-based leaf N estimation, sampling site on leaf, number of leaves sampled, and stage of plant development should be considered to minimize error.     

Leaf Chlorophyll Readings as an Indicator for Spinach Yield and Nutritional Quality with Different Nitrogen Fertilizer Applications

The objective of this study was to investigate the possibility of predicting the concentrations of total nitrogen (N), nitrate-nitrogen, and ascorbic acid in spinach (Spinacia oleracea) leaves using the pocket chlorophyll meter SPAD-502 (Minolta, Japan) in a pot experiment in a greenhouse. Spinach plants were grown in plastic pots filled with 0.5 kg of brown soil per pot with urea as N fertilizer at 0, 30, 60, 120, and 240 mg N/kg soil. SPAD readings of the two uppermost fully expanded leaves were recorded 18, 25, and 32 d after sowing and at harvesting (34 d). Dry-matter biomass and total N concentrations in leaves and roots, and NO₃-N, and ascorbic acid concentrations in leaves, were measured after harvesting. SPAD readings showed continuous reduction with increasing growth period irrespective of N applications. SPAD readings at harvest were significantly correlated with total N, leaf dry weight (DW), and NO₃-N concentration. However, this correlation did not exist between SPAD readings and ascorbic acid concentrations in leaves. The above results suggest that it is possible to apply SPAD readings to estimate NO₃-N concentrations in spinach plants, and that they may be applied for field assessments in decision-making and operational nutrient-management programs for the plant. Furthermore, the SPAD method may also be useful for ascertaining the harvest time. The results suggest that treatment with 120 mg N/kg significantly improved both leaf yields and leaf quality (i.e., leaf nitrate-N concentration and ascorbic acid). Too little and too much N fertilizer was not good for yield or spinach quality.     

Potential of sun hemp residue to provide potato with adequate nitrogen

Sun hemp (Crotalaria juncea L.) residue as a proper nitrogen (N) source for a potato crop (Solanum tuberosum L.) was investigated. Leaf nutritional indices (LNI), i.e, SPAD (SPAD-502, Minolta, Japan), total chlorophyll (CHLT), and N-total content, and their validity to predict tuber yield and N recovery were estimated. Moreover, comparison with mineral N fertilization was conducted. The estimated variables exhibited linear increase patterns to sun hemp N rates. Moreover, correlation coefficients between LNI and yield components were significant. Comparison of sun hemp N-amounts with mineral nitrogen showed lower SPAD values, similar CHLT, N-total, and N recovery. However, the elevated application produced superior yields to mineral one. Sun hemp residue incorporation prior to potato accounted for adequate N supply. Additionally, LNI are appropriate tools to determine potato N status and enable yield prognoses.     

Effects of nitrogen,phosphorus, and potassium on SPAD-502 and atLEAF sensor readings of Salvia

There are various optical sensors in the market for precision nitrogen (N) management, which estimate leaf N status from chlorophyll content of leaves. However, readings may also be affected by the amounts of other nutrients in the plant leaves like potassium (K) and phosphorus (P), as well as sampling location within a plant or leaf. SPAD-502 and atLEAF optical sensors were used on Salvia ‘Vista Red’ plants grown with 0N-7.9P-0K, 41N-0P-0K, 0N-0P-31.6K, 0N-7.9P-0K + 41N-0P-0K, 0N-7.9P-0K + 0N-0P-31.6K, 41N-0P-0K + 0N-0P-31.6K, or 0N-7.9P-0K + 41N-0P-0K + 0N-0P-31.6K fertilizer plus a control. Both sensors were correlated with leaf N and each other. However, both sensor readings were affected by the presence or absence of P and K in the leaves, and thus these nutrients should be analyzed and reported along with leaf N values. Sensor readings were found to vary within a leaf and within leaf canopy location, so sampling needs to be consistent.     

CALIBRATING CHLOROPHYLL METER (SPAD-502) READING BY SPECIFIC LEAF AREA FOR ESTIMATING LEAF NITROGEN CONCENTRATION IN SWEET SORGHUM

The chlorophyll meter (SPAD-502) is used to estimate nitrogen status of various crops. However, the relationship between SPAD readings and leaf nitrogen concentration (LNC) in sweet sorghum (Sorghum bicolor) has not been fully established. We examined the relationship between SPAD readings and LNC in sweet sorghum in a two-year study; and the effects of leaf thickness on the relationship was also examined. There was a significant relationship between the SPAD reading and LNC at each of two growth stages, but the correlation was weaker when the data for the two growth stages were pooled. This correlation improved when the specific leaf area was introduced as a second independent variable in the multiple regression analysis. This regression equation was applicable to not only different growth stages but also different seasons. The results suggest that the regression equation developed in this study can help in optimizing nitrogen fertilization for sweet sorghum production.     

DRIS Use on Apple Orchard Nutritional Evaluation in Response to Potassium Fertilization

Abstract

The aim of this work was to use the diagnosis and recommendation integrated system (DRIS) method to evaluate the nutritional status of an apple orchard, cv. Gala/MM106, at Vacaria, State of Rio Grande do Sul, Brazil. Treatments consisted of four levels of annual maintenance potassium fertilization. Data on fruit production and fruit pellicle color were restored from 1995 to 2000, as well as the data on leaf macro and micro-nutrient concentrations. The DRIS indices and nutritional balance index (NBI) for each nutrient were determined using three methods: Beaufils, Jones, and Elwali and Gascho. The results showed that (i) the NBI, calculated from the generated norms, were negatively correlated negative correlated with productivity and fruit coloration, and (ii) the DRIS method, described by Elwali and Gascho and using the F value, was the most suitable for apple tree orchards, because the NBI values obtained with this method best indicated the nutritional status of the plants and provided a more accurate nutritional diagnosis.     

Nutritional standards and nutritional diagnosis of the Conilon coffee plants in phenological growth stages

The objective of this study was to establish a nutrient sufficiency range and DRIS standards of Conilon coffee grown in southern Bahia, Brazil for the pre-flowering and grain-filling stages and to perform a nutritional diagnosis of these plants using different methods. The collections were performed in 24 Conilon coffee farm fields to establish the leaf nutritional standards, and 278 samples were collected to the nutritional diagnosis. The nutritional diagnosis was performed by using the sufficiency range criterion and the DRIS method. The nutrient sufficiency range and the DRIS standards must be specific to each phenological stage of the Conilon coffee plants. The leaf concentrations of calcium (Ca), sulfur (S), and boron (B) were higher in the pre-flowering stage; and the leaf concentrations of nitrogen (N), potassium (K), copper (Cu), and zinc (Zn) were higher in the grain-filling stage.     

Ranges of nutrient concentrations in Quercus ilex leaves at natural and urban sites

The leaf nutrient concentrations and the N‐to‐nutrient ratios were analyzed to evaluate the nutritional status of holm oaks (Quercus ilex L.) experiencing various anthropogenic pressures. Leaves (1 year old) of Q. ilex and surface soil (0–5 cm) surrounding the trees were collected at seven natural and seven urban sites in Campania Region (Southern Italy) and analyzed for the concentrations of macro (C, N, P, S) and micronutrients (Mn, K, Na, Cu, Mg, Ca, Fe, Zn). The available soil fraction of micronutrients was also evaluated. The nutrients showed different concentration ranges for the natural and the urban sites in the soil (total and available) and in the leaves, that we reported separately. Organic‐matter content and macronutrient concentrations were higher in the natural soils, while the highest leaf N, S, and P concentrations were found at some urban sites. Concentrations of Cu, Na and Zn both in leaves and soil, and Mg and Fe in leaves from the urban sites appeared to be affected by air depositions. Manganese was the only micronutrient to show higher concentrations at the natural than at the urban sites, both in soil and leaves. For this nutrient, in addition, a relationship between leaf and available soil concentrations was found at the natural sites. The ratios between the concentrations of N and each studied nutrient in the leaves highlighted a different nutritional status between the plants from the natural and urban sites.     

应用叶绿素仪诊断冬小麦氮营养状况的研究

利用叶绿素仪SPAD-502进行田间快速测定,并对作物进行氮肥推荐。结果表明,冬小麦应用叶绿素仪监测氮营养状况的测定部位为最上部完全展开叶的中部,该部位叶绿素仪测定值与作物全氮、施氮量及作物产量之间均有较好的相关性。作物品种对叶绿素测定结果影响很大,需要通过相对叶绿素仪读数的方法对其进行校正。     

小麦叶片水分及绿度特征的光谱法诊断

通过ASD FieldSpec光谱仪测定2个小麦品种3个氮肥处理、3个水分处理下的叶片反射光谱,用SPAD-502仪测定叶片的SPAD(绿度指数)值,并称取叶重。用ViewSpec Pro与Matlab软件处理光谱数据,分析光谱参数与叶片含水量及SPAD值的相关关系,从而明确叶片水分及绿度特征的最佳波段或光谱指数。结果表明,水分指数(WI)、水分胁迫指数(MSI)及中红外植被指数(MSVI1)与叶片含水量的相关关系密切且表现稳定,均通过了0.05水平的显著性检验;Fd664(664nm附近处一阶导数光谱值)、SDr/SDb(红边区域一阶微分总和与蓝边区域一阶微分总和的比值)与小麦叶片SPAD值的相关性达到极显著水平,因而利用光谱法诊断和监测小麦叶片水分及绿度特征具有良好的可行性,可为遥感技术应用于精准农业提供依据。     

EFFECT OF NITROGEN FERTILIZATION ON STAY-GREEN AND SENESCENT SORGHUM HYBRIDS IN SAND CULTURE

Nitrogen (N) is vital in the production of N containing compounds in cereal crops. Stay-greenness in sorghum [Sorghum bicolor (L.) Moench] is characterized by the plant's ability to retain water and chlorophyll content at maturity. A greenhouse experiment was conducted to investigate the effect of the genotypic variability between stay-green (BT × 643) and senescent (RT × 7000) hybrids for leaf senescence, dry matter partitioning and N partitioning under three N levels (0.45, 0.90, 1.80 g plant^?1). The stay-green hybrid had a higher percentage green leaf area at 120 DAE with a higher residual N in leaves. The chlorophyll meter (SPAD-502) values for the stay-green hybrid during anthesis (75 and 85 DAE) were higher compared to its senescent counterpart. The results indicated that the expression of stay-green phenotype in sorghum was contingent on a minimum N supply of 1.35 g plant^?1 and associated with greater N acquisition. Nitrogen use and uptake in the stay-green hybrid were higher.     

Standardization of Leaf Sampling Technique in Bael

Abstract

The chemical diagnosis of nutritional status of Bael (Aegle marmelos Correa) tree by leaf analysis was done to study the effect of leaf age and leaf position on foliar nutrient composition of Bael. The results showed that leaf age has significant influence on leaf mineral content, whereas the position of leaf on shoot did not have any significant effect. Interactions between leaf age and position were found to be significant for nitrogen, boron and iron content. In general, stability in level of nitrogen (N), phosphorus (P), and potassium (K) contents was observed in November–December (6‐ to 7‐month‐old leaves) and calcium (Ca) and magnesium (Mg) showed their stability in 2‐to 3‐month‐old leaves (July–August). The contents of boron (B), manganese (Mn), copper (Cu), and zinc (Zn) were found stabilized in September–October (3‐ to 4‐month‐old leaves), indicating their suitability for sampling in Bael.