Physiological responses of pepper plant (Capsicum annuum L.) to drought stress

Water shortage is the most important factor constraining agricultural production all over the world. New irrigation strategies must be established to use the limited water resources more efficiently. This study was carried out in a completely randomized design with three replications under the greenhouse condition at Shahrekord University, Shahrekord, Iran. In this study, the physiological responses of pepper plant affected by irrigation water were investigated. Irrigation treatments included control [full irrigation (FI) level] and three deficit irrigation (DI) levels—80, 60, and 40% of the plant's water requirement called DI₈₀, DI₆₀, and DI₄₀, respectively. A no plant cover treatment with three replications was also used to measure evaporation from the soil surface. Daily measurements of volumetric soil moisture (VSM) were made at each 10-cm intervals of the soil column, considered as a layer. The differences between the measured VSM and the VSM in the next day and evaporation rate at the soil surface at the same layer of the bare soil with no plant cover treatment were calculated. Eventually, by considering the applied and collected water in each treatment, evapotranspiration (ET_C) and root water uptake in each layer per day were estimated. Furthermore, fruit number per plant, fresh fruit weight/day, root fresh/dry weights, shoot fresh/dry weights, root zone volume, root length and density, crop yield, and water use efficiency (WUE) were measured under different water treatments. The results showed that the maximum and minimum of all the studied parameters were found in the FI and DI₄₀ treatments, respectively. ET_C in the DI₈₀, DI₆₀, and DI₄₀ treatments were reduced by 14.2, 37.4, and 52.2%, respectively. Furthermore, applying 80, 60, and 40% of the plant's water requirement led to the reduction in crop yield by 29.4, 52.7, and 69.5%, respectively. The averages of root water uptakes in the DI₈₀, DI₆₀, and DI₄₀ treatments reduced by 17.08, 48.72, and 68.25%, respectively. WUE and crop yield also showed no significant difference in the FI and DI₈₀ treatments. Moreover, in the DI₈₀ treatment, the reduced rate of water uptake was less than the reduced rate of plant's applied water. According to these results, it can be concluded that 20% DI had no significant reduction on the yield of pepper, but above this threshold, there was an adverse effect on the growth and yield. Therefore, for water management in the regions with limited water resources, rate of plant's applied water can be decreased by around 20%.     

Root water uptake of pepper plants (Capsicum annuum L.) under deficit irrigation system

Root water uptake is a component of water balance that has not been clearly understood. This study was carried out in a completely randomized design with three replications under the greenhouse condition at Shahrekord University, Shahrekord, Iran. In this study, the root water uptake (RWU) by pepper plant under various irrigation water levels was investigated. Irrigation treatments included control (full irrigation level, FI) and three deficit irrigation levels, 80%, 60% and 40% of the plant's water requirement called DI₈₀, DI₆₀ and DI₄₀, respectively. A no-plant cover treatment with three replications was also used to measure evaporation from the soil surface. Daily measurements of volumetric soil moisture (VSM) were made at 10 cm intervals of the soil column. The differences between the measured VSM and the VSM in the next day, and evaporation rate at the soil surface at the same layer of the no-plant cover treatment were calculated and, eventually, the RWU in each layer per day was estimated. The results showed that the maximum and minimum RWUs were found in the FI and DI₄₀ treatments, respectively. The averages of root water uptakes in the DI₈₀, DI₆₀, and DI₄₀ treatments were reduced by 17.08%, 48.72% and 68.25%, respectively. Furthermore, in the DI₈₀ treatment, the reduced rate of water uptake was less than the reduced rate of water applied to the plants.     

Effects of bicarbonate and different Fe sources on vegetative growth and physiological characteristics of bell pepper (Capsicum annuum L.) plants in hydroponic system

In order to determine the best iron (Fe) sources under alkaline conditions, an factorial experiment was conducted based on a completely randomized design with two factors of Fe fertilizer at four forms [iron sulfate (FeSO₄), Fe- ethylenediaminetetraacetic acid (EDTA), Fe- diethylenetriaminepentaacetic acid (DTPA) and Fe- ethylenediamine-N,N’-bis (EDDHA), and sodium bicarbonate (NaHCO₃)] at three levels (0, 10 and 15 mM) with three replications. Results showed that the highest loss of vegetative growth (stem length, leaf number, leaf area, stem diameter, and leaf, stem and root dry weight) and ecophysiological parameters (F_v/F_m, SPAD and RWC) was observed in plants treated with FeSO₄. Alkalinity stress increased proline concentration especially in FeSO₄ treatment. Bicarbonate treatments decreased Fe concentration in plant tissues. Fe-EDTA and Fe-DTPA fertilizer sources acted similar or even better than EDDHA at 10 mM NaHCO₃ concentration, but the best Fe fertilizer source was Fe-EDDHA at 15 mM NaHCO₃ concentration.     

Effect of drip irrigation and mulches on physiological parameters,soil temperature,picking patterns and yield in capsicum (Capsicum annuum L.)

Water and temperature are critical for producing vegetable crops, especially during winter, when the availability of water is meager and temperature falls. Studies of drip irrigation and mulch were undertaken to find the effect on different growth and yield parameters in pepper (Capsicum annuum L.). The experiment was laid out in a split-plot design with four main and four subplots. Irrigation levels were placed on main plots and mulches on subplots with three replicates for each. All recorded vegetative parameters were higher with drip irrigation at 1.0 pan evaporation (Epan) and black polythene mulch. Physiological parameters such as photosynthesis rate (18.01 and 17.45 μmol m^?2 s^?1), transpiration rate (6.19 and 5.86 mmol H₂O m^?2 s^?1) and chlorophyll content (27.34 and 28.39; 39.22 and 41.27 SPAD, respectively at 50 and 100 days after planting) were maximal in crops with drip irrigation at 1.0 Epan and mulched with black polythene. Soil and canopy temperature were significantly higher on flood irrigation at 1.0 Epan. Soil temperature was higher with the black polythene mulch, but canopy temperature was higher with no mulch. A higher level of drip irrigation and black polythene mulch result in early picking with higher yields compared with flood irrigation and no mulch.     

Structure-reactivity relationship in the oxidation of carotenoid pigments of the pepper (Capsicum annuum L.)

The relationship between the degradation rate and structure of each pigment of the pepper carotenoid profile was studied in mixtures of dehydrated fruit with lipid substrates of differing degrees of unsaturation and in different proportions (20 and 40%). The differences in structural nature of the carotenoids present in the pepper fruit produce a variable rate of oxidation, resulting in nonuniform degradation. The yellow xanthophylls and beta-carotene have the highest rates of oxidation, with the ketocarotenoids and violaxanthin degrading at lower rates. Autoxidation is greater or lesser depending on the functional groups, which stabilize the radical intermediaries of the reaction. The behavior of capsanthin and capsorubin is that expected of carotenoids having structures that include keto groups: a markedly greater stability to autoxidation processes. This increases their antioxidant capacity, adding to their beneficial impact by reducing the proliferation of radical processes, which are detrimental to health.     

Effect of Pseudomonas putida and inorganic fertilizer on growth and productivity of habanero pepper (Capsicum Chinense Jacq.) in greenhouse

The habanero pepper is widely used in a variety of commercial food products. The increase in the cultivation area of the habanero pepper has increased the use of agricultural inputs, mainly inorganic fertilizers, which have high economic costs and cause negative effects to the soil and water. In recent years, the use of beneficial soil microorganisms has been studied as an alternative to inorganic fertilizers. In the present study, the effect of three strains of Pseudomonas putida on the growth and productivity of King Pakal hybrid habanero pepper with limited inorganic fertilizer in a greenhouse. It was found that a reduction of inorganic fertilizer application of 25% with P. putida rhizobacteria strains increased or maintained growth and productivity of King Pakal hybrid habanero pepper. Before these rhizobacteria can be used in the field, results will first need to be confirmed under more natural conditions.     

不同灌水模式对南方辣椒叶绿素荧光参数的影响

为了实现辣椒节水、优质、高效生产,在避雨栽培条件下,利用OS5-FL调制荧光仪研究了不同灌水模式对辣椒叶绿素荧光参数的影响。结果表明,在4个生育阶段,辣椒的可变荧光、最大荧光、可变荧光与最大荧光比、可变荧光与初始荧光比、光化学淬灭系数与非光化学淬灭系数均随着灌水量的减少而降低,而初始荧光则随着灌水量的减少而升高。说明辣椒叶片PSⅡ反应中心因水分胁迫而受到了破坏,使PSⅡ原初光能转换效率和PSⅡ潜在活性降低,使光合电子传递、光合原初反应过程及热耗散能力受到抑制。DI50、2PRD和1PRD 3种处理下的辣椒各叶绿素荧光参数虽然在4次观测中均未表现出显著差异,但1PRD处理与对照相比变化最小。该研究可为南方避雨栽培下辣椒灌水模式的选择和节水、优质、高产辣椒栽培提供参考。     

施钾对不同基因型棉花光合特性及产量和品质的影响

在田间条件下,研究了施钾对抗虫杂交棉中棉所38、抗虫棉新棉33B、常规棉中棉所12光合特性和产量及品质的影响。结果表明,与不施钾相比,不同基因型棉花施氯化钾225.kg/hm2,棉花叶面积指数(LAI),叶绿素荧光动力学参数PSⅡ最大光化学量子效率(Fv/Fm)、PSⅡ潜在光化学活性(Fv/Fo)、PSⅡ实际光化学效率(ФPSⅡ),叶片净光合速率(Pn)、气孔导度(Gs),棉花干物重及分配到生殖器官的比例,棉花总成铃数、铃重和衣分均有不同程度提高;中棉所38、新棉33B和中棉所12皮棉产量分别增加13.60%、10.66%和4.21%;棉纤维2.5%跨距长度、比强度、马克隆值和纤维整齐度等品质也得到改善。中棉所38和新棉33B比中棉所12施钾的效果更好,生产上应优先给抗虫杂交棉和抗虫棉品种施用钾肥。     

农膜残留对大豆光生理特征及生物量累积的影响

农膜覆盖技术的应用及推广极大地提高了干旱半干旱地区的农业产量,促进了当地农业发展及社会经济效益。然而,由于农膜碎片化程度高、回收难度大、降解周期长,使得残留在土壤中的农膜日益增多,严重威胁着作物生长、土壤健康以及农业可持续发展。尽管农膜残留对土壤质量影响的研究较多,但对于其种类(可降解或不可降解)及残留累积量对作物光生理特征的研究还相对较少。本试验以大豆为研究对象,对比普通聚乙烯(PE)和生物降解(BP)两种农膜(残片大小为0.5～2 cm),研究不同农膜残留累积量(土壤重量的0、0.1%、0.5%、1.0%)下大豆花期及初荚期叶片光合作用光、CO_2响应曲线特征及花期、收获期的植株生物量,探讨塑料类型及残留量对大豆光生理特征及生物量累积的影响。结果表明:PE残留导致大豆叶片光补偿点在花期降低23.96%,而初荚期升高51.38%,说明PE残留导致大豆叶片弱光利用能力在花期提升,但在初荚期被抑制。在初荚期,BP残留使光补偿点降低54.82%,且光饱合点升高58.12%,从而提高了叶片强光适应能力,增大了叶片光能利用范围。同时, PE和BP添加使暗呼吸速率分别增长30.56%和22.28%,从而导致干物质消耗增加。土壤中PE、BP残留量的增加,最大光合力分别降低36.49%和23.56%,表明大豆叶片CO_2利用能力减弱; CO_2补偿点分别降低67.96%和38.91%,从而提高了叶片低浓度CO_2的利用能力,并降低光呼吸速率,从而减少了干物质的消耗。此外,不同农膜及残留量处理下,仅在花期0.1%与0.5%残留量的BP处理中,地下生物量随农膜残留量的增加显著降低,其他各处理间地上及地下生物量无明显变化。光响应及CO_2响应曲线各拟合参数与生物量的Pearson相关性分析结果表明,收获期PE处理下,地上生物量与光补偿点呈显著负相关,而光呼吸速率、CO_2补偿点、初始羧化效率与生物量(地上+地下)的积累有较强相关性。因此,PE农膜残留量增加提高了大豆花期叶片对于弱光的利用能力而减弱初荚期对弱光的利用能力,BP农膜残留量增加则会增强初荚期叶片对弱光的利用,也对大豆叶片适应强光的能力有所提升。     

覆黑地膜对旱作玉米根区土壤温湿度和光合特性的影响

明确雨养旱地地膜覆盖春玉米高产与水分高效利用的土壤环境条件,及其对玉米光合特性的作用,对延缓玉米衰老、提高玉米光合能力和促进玉米增产具有重要意义。以陕单609为材料,2012和2013年开展黑色地膜覆盖、白色地膜覆盖和裸地栽培试验,研究黑色地膜对玉米根区土壤水温环境、玉米叶片光合特性、产量和水分利用效率的影响。结果表明,黑色地膜较普通白色地膜和裸地栽培,能显著改善玉米根区土壤温湿条件,提高玉米叶片光合能力。黑色地膜覆盖下,玉米叶片衰老时间明显推迟,衰老速度显著下降,成熟期叶片叶绿素含量较普通白色地膜提高11.7%(P0.05),较裸地提高45.5%(P0.05)。与普通白色地膜和裸地栽培相比,黑色地膜覆盖提高了吐丝后玉米叶片光合速率、蒸腾速率、叶片水分利用效率、PSII电子传递速率、量子产量(灌浆期除外)和光化学猝灭系数。黑色地膜覆盖下玉米千粒质量、产量和水分利用效率显著增加,千粒质量较普通白色地膜和裸地栽培分别增加5.0%和14.9%(P0.05);产量分别提高10.4%和22.1%(P0.05);水分利用效率分别提高10.4%和25.3%(P0.05)。在雨养旱作地区,玉米采用黑色地膜覆盖具有延缓后期叶片衰老和促进增产的效果。     

Purification of hydroperoxide lyase from green bell pepper (Capsicum annuum L.) fruits for the generation of C6-aldehydes in vitro

The aim of this work was to compare the efficiency of different extracts of hydroperoxide lyase from green bell peppers in producing aldehydes: a crude extract, a chloroplastic fraction, and a purified enzyme were investigated. From a crude extract, the HPO lyase was purified by ion-exchange chromatography with a 22.3-fold increase in purification factor. Analysis by SDS-PAGE electrophoresis under denaturating conditions showed only one protein with a molecular weight of 55 kDa, whereas size-exclusion chromatography indicated a molecular weight of 170 kDa. A maximum of 7500 mg of aldehydes per g of protein was obtained with the purified enzyme within 20 min of bioconversion compared to 392 and 88 mg of aldehydes per g of protein within 50 and 60 min, respectively, for the chloroplast fraction and the crude extract.     

不同基质对小青菜穴盘苗生长和光合特性的影响

为研究不同类型基质对穴盘栽培小青菜幼苗生长和光合特性的影响,选用了两种规格的穴盘和3种类型基质(草炭、珍珠岩及园土),将这3种基质按不同体积比配制成5种混合基质,即草炭(CK)、草炭∶珍珠岩=1∶1(A1)、草炭∶珍珠岩=3∶1(A2)、草炭∶珍珠岩∶园土=1∶1∶1(A3)、草炭∶珍珠岩∶园土=2∶1∶2(A4),从中筛选出适宜小青菜生长的栽培基质。结果表明:对两种规格栽培的小青菜,中容重、有效含水量、全氮、磷、钾含量较高的基质,尤其是A4处理更适合小青菜穴盘幼苗的生长;基质总孔隙度越高,气孔导度越低,小青菜的光合速率和蒸腾速率也越低,A4处理光合速率、蒸腾速率和气孔导度都最大,因此,A4处理基质最有利于小青菜穴盘幼苗生长。     

地面覆盖对温室黄瓜生产及水分利用效率的影响

试验共设地面覆盖秸秆、覆盖地膜、覆盖秸秆+地膜以及对照四个处理,采用随机区组设计。研究了不同地面覆盖对日光温室黄瓜生产及水分利用效率的影响,以期为日光温室黄瓜生产筛选最佳覆盖模式,为发展节水型生态农业提供理论依据和技术支持。结果表明：①地面覆盖能有效促进植株与瓜条生长发育,提高正常瓜比例,其中以覆盖秸秆+地膜效果最好;②地面覆盖的黄瓜产量显著高于对照,覆盖秸秆+地膜在提高早期产量与总产量的效果最好,覆盖地膜在提高早期产量上优于覆盖秸秆,但后期产量远低于覆盖秸秆处理,总产量低于覆盖秸秆处理;③地面覆盖可有效改善瓜条品质,增加黄瓜的干物质含量、可溶性糖含量、维生素C含量、可溶性蛋白质含量及游离氨基酸总量,且以覆盖秸秆+地膜效果最好;④覆盖秸秆、覆盖地膜与覆盖秸秆+地膜的水分利用效率极显著高于对照,分别较对照提高74.2%、112.5%与102.3%,覆盖地膜与覆盖秸秆+地膜的水分利用效率显著高于覆盖秸秆处理。     

Response of soyabean (Glycine max Mer.) to wheat straw mulching in different cropping seasons

Abstract. The favoured temperature range for soyabean seed germination is 25–30 °C and the crop is sensitive to water stress. In northern India, the crop is sown in the hot-dry months of May–June. Straw mulching can alter the soil's hydrothermal regime by lowering the temperature and reducing evaporation losses. A field experiment was conducted from 1999 to 2002 at the Research Farm of Punjab Agricultural University, Ludhiana on a loamy sand soil to evaluate the effect of wheat straw mulch on soil temperature, soyabean seed yield and crop growth. Maximum soil temperatures at sowing depth, recorded during the 1-month period after sowing (seed germination to seedling establishment stage), were high under no-mulch, ranging from 30.6 to 48.6 °C, while mulching substantially reduced these temperatures by 1.4 to 12.7 °C. Mulching increased soyabean seed yield by 4.4 to 68.3% in different cropping seasons; it also increased plant biomass by 17 to 122% and nodule mass by 8 to 220%. Leaf area index, chlorophyll content of leaves and number of pods per plant were all increased. Seed yield improvement under mulch was negatively correlated with rainfall distribution (number of rainy days) and amount during the whole cropping season. The percentage increase in seed yield with mulching was regressed against the total number of rainy days and total rainfall in millimetres in the cropping season. Thereby 93% of the variation in response to mulching in different cropping seasons was explained by the distribution and amount of rainfall.     

RAPD and AFLP assessment of genetic variation in a landrace of pepper (Capsicum annuum L.), grown in North-West Italy

In several regions of Italy as well as other parts of southern Europe, the heterogeneity of the land, the climate and the soil favour the survival in cultivation of a large number of landraces specifically adapted to local conditions. Knowledge on the level and distribution of their genetic variation can help to develop appropriate strategies, in order to suistainably manage in situ these germplasm resources at risk of genetic erosion. C. annuum is an herbaceous diploid species and is considered to be self-pollinating, although different rates of out-crossing have been recorded. We used random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers to assess genetic diversity within and between five populations of a landrace of Capsicum annuum L., grown in a limited area in north-west Italy and locally known as Cuneo pepper. Partitioning the genetic variation with Shannon's diversity index revealed that 41.6% occurred between and 58.4% within populations. Analogous results were obtained when the analysis was based only on RAPD or AFLP markers. However, AFLP was more reliable, since a lower range of variation was observed among primer combinations in detecting the two components of genetic variation. Notwithstanding the rather high level of within genetic variation detected, the five populations were clearly differentiated and differed in the frequency of alleles exclusive and/or present at very low frequencies. Our results show the need for accurate estimation of allele frequencies, in order to identify populations to which priority should be given for dynamic conservation of landraces.     

西北旱作农田不同耕作模式对土壤性状及小麦产量的影响

【目的】在雨养农业区,旱作区因连年翻耕而引起严重的土壤质量退化,使作物生产力下降,需定期改变其耕作方式。免耕深松隔年轮耕可以降低土壤容重,增加耕层土壤团聚体和有机碳氮的含量,增强土壤蓄水保墒能力,对改善土壤性状和提高作物产量具有重要意义。【方法】本研究于2007～2010年在宁夏南部半旱区进行了两年免耕一年深松 (NT/ST/NT)、两年深松一年免耕 (ST/NT/ST)、连年翻耕 (CT) 3种耕作模式试验,研究了其对耕层土壤容重、团聚体、土壤有机碳氮含量、土壤水分及作物产量的影响。【结果】3年耕作处理后,与连年翻耕相比,NT/ST/NT、ST/NT/ST处理0—20 cm层土壤容重分别降低了4.4%和7.3%,20—40 cm土层分别降低2.1%和5.7%,40—60 cm土层分别降低4.1%和5.5%;土壤孔隙度0—20 cm土层分别提高了4.1%和6.8%,20—40 cm土层提高了2.1%和4.3%,40—60 cm土层提高了5.5%和5.7%。0—20 cm土层,NT/ST/NT处理0.25～2 mm机械稳定性团聚体含量平均较CT处理提高了12.4%,ST/NT/ST处理 > 2 mm机械稳定性团聚体含量较CT处理平均提高了42.0%;20—40 cm土层,NT/ST/NT、ST/NT/ST处理 > 2 mm团聚体含量较CT处理平均分别提高了44.3%和50.4%。两种轮耕模式使0—40 cm土层土壤团聚体平均重量直径分别显著高于CT处理21.8%和22.5%,几何平均直径分别高于CT处理9.6%和9.5%。三个处理耕层土壤有机碳氮含量均比试验前有不同程度的增加,轮耕处理0—30 cm土层0.25～2 mm粒级有机碳含量和 < 0.25 mm粒级全氮含量显著高于CT,以ST/NT/ST处理效果最佳。NT/ST/NT和ST/NT/ST处理0—10 cm土层0.25～2 mm团聚体有机碳含量较CT处理分别显著提高7.9%和10.2%,10—20 cm土层分别提高19.0%和15.7%,20—30 cm土层分别提高10.6%和13.3%;0—10 cm土层 < 0.25 mm粒级全氮含量显著提高9.4%和10.9%,10—20 cm土层分别提高6.8%和10.2%,20—30 cm土层分别提高7.4%和9.3%。研究期间,NT/ST/NT和ST/NT/ST处理较CT处理可显著提高0—200 cm土壤贮水量,其中以ST/NT/ST处理保蓄土壤水分效果最佳。在小麦生长前期,轮耕处理土壤贮水量均高于连年翻耕,生长后期ST/NT/ST处理土壤水分含量最高,NT/ST/NT处理次之。轮耕处理的小麦生物量和籽粒产量显著高于连年翻耕,其中小麦籽粒产量分别增加9.6%和10.7%。【结论】免耕/深松轮耕可显著改善土壤的物理性状和水分环境,显著增加耕层土壤有机碳氮含量,提高作物的生产力,在宁南旱区有重要的应用前景。     

陇东旱地苹果园不同地面覆盖模式的水分与养分效应

为完善和改进果园树盘起垄覆膜集雨保墒模式,提高土壤墒情,探寻更加有效的果园保墒措施,该研究以17 a生苹果园为研究对象,设置常规树盘起垄覆膜行间清耕模式（F）,在F 基础进行改进的树盘起垄覆膜+行间覆草模式（F+W）和树盘起垄覆膜+行间覆草+膜侧添加渗水穴模式（F+W+H）2种改进模式,以不起垄不覆盖为对照（CK）,采用定点试验,连续3 a(2015年干旱、2016年秋旱和2017年常年降雨)开展苹果花前、果实膨大期及采后的水分效应,以及养分效应和树体生长发育响应研究。结果显示：3种覆盖模式可不同程度增加0～200 cm土层的含水率, 以2017年最显著,2种改进模式可缓解300～500 cm土层水分消耗,降低休眠期无效蒸散,3 a三种覆盖模式果园土层总蒸散量均极显著（P<0.01）低于CK,由低到高顺序为F+W+H、F+W、F、CK。2015年和2016年采后覆盖模式0～300 cm土层硝态氮累积量均极显著（P<0.01）低于CK（比CK低34.4%～39.6%）。3种覆盖模式增加果实中氮磷钾养分吸收,以钾最突出。F+W+H、F+W和F 3 a平均产量分别较CK提高13.1%、13.0%和8.3%。综合各种效应,以2种改进覆盖保墒模式F+W+H和F+W较优。     

不同覆膜栽培方式对玉米干物质积累及产量的影响

为探讨地膜覆盖栽培方式的增产机理,完善地膜覆盖玉米栽培技术,采用田间试验法,以露地平播为对照,比较了全膜双垄沟播、半膜双垄沟播、半膜平铺、膜侧沟播等4种栽培方式对玉米叶面积、光合势、干物质积累、穗部性状、产量及其构成因子、水分利用效率的影响,并进行了成本和效益分析。结果表明:全膜或半膜双垄沟播显著增加了各个生育时期玉米的叶面积,半膜平铺和膜侧沟播作用不大;全膜和半膜双垄沟播及膜侧沟播显著提高了玉米的光合势,半膜平铺对生长前期光合势有一定促进作用,但后期出现不利影响;全膜和半膜双垄沟播增加了玉米穗长、穗粗、穗行数和行粒数,但也增加了秃顶长。半膜平铺对穗长、穗粗和穗行数有一定促进作用,降低了秃顶长,但对行粒数作用并不明显。膜侧沟播对穗长、穗行数和行粒数有一定促进作用,但对穗粗和秃顶长作用并不明显;各种覆膜栽培方式均提高了玉米穗粒数和百粒重,但降低了单株成穗数。穗粒数与产量的灰色关联度最大、百粒重次之、单株成穗数最低;全膜双垄沟播、半膜双垄沟播、膜侧沟播、半膜平铺栽培方式分别比对照增产67.23%、60.08%、38.81%和20.02%,水分利用效率分别比对照提高66.43%、59.54%、38.35%和19.63%,经济收益分别比对照增加85.51%、69.58%、36.37%和18.69%。说明全膜双垄沟播玉米具有较高的产量和经济收益优势。     

Effect of farmers' seed selection on genetic variation of a landrace population of pepper (Capsicum annuum L.), grown in North-West Italy

Recognition by the European Community of élite landraces encourages farmers to grow these and earn more than is possible by growing modern varieties. However, farmers often exercise strong selection by collecting seed from a limited number of plants which best embody a few characters of prime interest. This, in the long run, may be responsible for considerable genetic erosion. In Southern Piedmont, North-West Italy, a local landrace of pepper (Capsicum annuum L.) known as Cuneo is grown; it deserves particular attention because of its hardiness, late production and fruit quality. We used random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers to assess the effect, on genetic composition of a population, of seed sampling carried out using the selection criteria adopted by the farmer. After two reproductive cycles using selection, it was already possible to detect loss in genetic variation and a change in allele frequencies, while no significant effect was found after two cycles of random sampling. Over this period, farmer selection pressure led to disappearance of eight low-frequency alleles, while only three alleles were lost in randomly sampled populations. Our results stress the need to adopt appropriate techniques for seed sampling in order to avoid genetic erosion of local landrace gene pools.