Varietal differences in accumulation of ochratoxin a in barley and wheat cultivars after inoculation of Penicillium verrucosum

The natural content of ochratoxin A in grain samples of 6 barley, 2 bread wheat and 1 durum wheat cultivars varied from <0.1 to 0.4 ng/g grain. Samples of the analysed cultivars were surface sterilized and kept in humidity chambers at 20°C and water activity (a_w) 0.75 or a_w 0.85 for 8 days. For both environments, the resulting grain equilibrium water content varied between cultivars of both barley and wheat, attributable to agronomic traits. The samples were then inoculated with Penicillium verrucosum and incubated for up to 23 weeks. With time, all cultivars had increasing ochratoxin A content, with maximum content in different barley cultivars ranging from 34 to 630 ng/g grain for a_w 0.75, and 39 to 260 000 ng/g for a_w 0.85. Corresponding values for the wheat cultivars were 25 to 2 300 ng/g and 650 to 5 200 ng/g. Significant varietal differences in ochratoxin A accumulation were observed for barley (P < 0.0001), attributable to equilibrium water content, amylose content and natural ochratoxin A, and for wheat (P < 0.0001), attributable to protein content and natural ochratoxin A. Barley ‘SW 1306 95/1203’ and ‘SW 906129 Waxy’, and wheat ‘SW 39103’ accumulated significantly less ochratoxin A than the other cultivars.     

Aluminum tolerance and micronutrient accumulation in cereal species contrasting in iron efficiency

Aluminum (Al) negatively interferes with the uptake or transport of different nutrients. The aim of our work was to compare the Al tolerance and micronutrient accumulation: iron (Fe), zinc (Zn) and manganese (Mn), in cereal species (winter wheat, spring wheat, winter rye, oats and barley) contrasting in Fe efficiency. Our previous screening in a calcareous soil showed that oats and barley were more Fe-efficient than spring wheat, winter wheat or winter rye. In Al stress conditions, both oats and barley exhibited more effectiveness in Fe acquisition and translocation from root to shoot in comparison to winter wheat, spring wheat and winter rye. Also, oats and barley responded to Al toxicity by less Al-retarded shoot biomass than other cereal species. A combination of tolerance mechanisms appears to have great importance for Al tolerance including mechanisms underlying Fe efficiency in cereal seedlings.     

A field study on factors influencing Cd levels in soils and in grain of oats and winter wheat

The influence of selected factors on Cd levels in soils and in grain of oats and winter wheat was investigated. Soil and grain were sampled at sites randomly distributed over Sweden. Organic soils generally had higher Cd contents and lower pH levels than mineral soils, and plants growing in organic soils tended to have higher Cd contents than plants growing in mineral soils. In mineral soils the amount of soil Cd extractable in 2M HNO₃ was positively correlated with the pH and the contents of organic matter and clay. The studied variable best correlated with the Cd content of oat grain grown on mineral soils was the pH (negative correlation). Soil contents of organic matter, clay, HNO₃-extractable Cd and Zn were also found to be significantly related to the Cd content in a stepwise regression analysis. In winter wheat grain, Cd content was best correlated with the HNO₃-extractable Cd (positive correlation). Additional significant factors were pH, grain yield and contents of organic matter, clay and HNO₃-extractable Zn. In winter wheat the presence of Zn reduced Cd uptake, and vice versa; no such mutually antagonistic relationship was apparent in oats. In oats, but not in wheat, it was possible to predict most of the differences in grain Cd content, caused by the factors described above, based on the variation in CaCl₂-extractable soil Cd. Analysis of samples from field trials indicated that there were differences in Cd content between varieties of both crops. Variation in factors described generally explained most of the differences in soil- and grain Cd levels between regions.     

Fusarium seedling blight of wheat and oats: effects of infection level and fungicide seed treatments on agronomic characters

The effects of seed-borne Fusarium spp. and Microdochium nivale infection in spring wheat, winter wheat, and oats in Sweden was investigated in field trials for the agronomic characters yield, thousand kernel weight, grain volume weight, gluten, protein, starch, straw strength, and plant density. Seed with high and low levels of infection was mixed to obtain six infection levels in the proportions of 100/0, 80/20, 60/40, 40/60, 20/80, and 0/100 percent. The seed was untreated or treated with Celest Extra Formula M (CEFM, difenoconazole + fludioxonil) or Celest Formula M (CFM, fludioxonil). In the field trials using untreated seed, there were significant differences between infection levels only for some agronomic characters and levels. Fungicide seed treatment with CEFM in spring wheat had no significant effect on most agronomic characters including yield. In winter wheat and oats, seed treatment with CFM increased yield by 7–11% and plant density by up to 33% while having no effect on other characters. The percentage discoloration of crown roots and stem bases due to Fusarium/Microdochium spp. was also investigated visually in winter wheat and oats and found to increase with higher infection level. Fungicide seed treatment thus mainly increased plant emergence in seed lots with low-to-moderate Fusarium/Microdochium spp. infection and had little or no effects on other agronomic characters.     

Ergosterol Levels and Mould Colony Forming Units in Swedish Grains of Food and Feed Grade

Abstract

Although ergosterol is considered to be a suitable indicator of mould growth in cereal grains, there are few reference values available for Scandinavian conditions. We have determined the ergosterol levels in Swedish grain of different origins: cleaned food-grade wheat from a commercial mill, feed-grade cereals (oats and barley) with different odours and cereals (winter wheat, “American wheat”, triticale and rye) from various field trials conducted in south-central Sweden in 1990. Specific objectives were to elucidate the relationships between ergosterol levels and numbers of mould colony forming units (CFU) and between ergosterol and grain odour.

Ergosterol levels in the food-grade wheat ranged between 2.4 and 2.8 μg/g DW, and between 3.0 and 5.6 μg/g DW in the field trial cereals, while values in most of the feed grain samples ranged from 8–15 μg/g DW. The levels agree with other published data for European grains.

A positive correlation was found between numbers of colony-forming units and ergosterol concentration. The degree of correlation was higher when numbers of CFU were determined on dichloran-glycerol 18% agar with a low water activity (aw = 0.95) than on malt extract agar (aw = 0.99). There was no agreement between ergosterol levels and grain odour, since even samples described as having a fresh smell had high ergosterol levels. However, the highest level (33 μg/g DW) was found in a sample with a pronounced musty odour, and the lowest (1.1 μg/g DW) in a sample that smelled as if it had been heat damaged.     

Economic and environmental optimization of nitrogen fertilizer recommendations for cereals in Norway

Abstract

Results of 240 annual N fertilizer trials in 1991–2007 in spring and winter cereals are presented. On average, spring barley and oat yields increased little beyond 120 kg N ha^?1 in fertilizer. Somewhat higher figures were found for spring and winter wheat. Regression equations for yield and N uptakes in grain and straw were derived, related to N fertilizer input and the yield level in individual trials (indicator of yield expectancy). These equations accounted for 90% of the variation in yield and 80% of that in N uptake. Quadratic N responses were significant in all cases, as were interactions between N responses and yield level. They were verified with data from 27 separate trials performed in 2008–2010. The yield equations were used to calculate economically optimum N fertilizer levels with varying ratios of product price to fertilizer cost at contrasting levels of yield. The optimum N fertilizer level for barley and oats was found to increase by 8.3 kg N ha^?1 per Mg increase in expected yield. The equivalent figure in wheat was 16.3 kg N ha^?1. Optimum N fertilizer levels decreased by 4.1 and 6.7 kg N ha^?1, for barley/oats and wheat respectively, per unit increase in the cost/price ratio. The equations for N uptake were used to calculate simple N balances between fertilizer input and removal in crop products. Large N surpluses were indicated at low levels of yield expectancy, but the surplus declined markedly with increasing yield level, despite greater N fertilizer inputs at high yield. Calculations made for national average yield levels in recent years showed N surpluses of 50–60 kg N ha^?1 when only grain is removed and 25–40 kg N ha^?1 when straw is removed also. Limiting N input to obtain zero balance reduces yields considerably at average levels of yield expectancy.     

Der Mangan-Gehalt von Getreide,Mais und Rüben als Anzeiger der Bodenversauerung

Manganese content of cereals, maize and beet as indicator of soil acidity Soil and plant samples with and without growth depressions were taken from 479 agricultural fields (sand - loam) in the former GDR and analysed for pH and Mn and Mg respectively. Depressions in growth accompanied with symptoms of Mn excess and sometimes also of Mg deficiency were observed for barley, wheat, oats, maize and beets at soil pH < 4, 7, for rye at pH < 4, 1 - 4, 4. Soil pH correlated highly negatively with the Mn concentration of plants in early growth stages. Thus toxicity levels of Mn concentration in young plants could be derived. Mn concentrations > 140 mg/kg dry matter in spring barley, > 150 mg/kg in winter barley, > 160 mg/kg in wheat, > 200 mg/kg in rye, > 300 mg/kg in oats, > 350 mg/kg in maize and > 800 mg/kg in beet indicate growth depressions because of soil acidity. In the case of simultaneous Mg deficiency these Mn values are lower for oats and rye.     

Crop rotation effects on yield of oilseed rape,wheat and barley and residual effects on the subsequent wheat

Economic conditions are forcing farmers to grow crops with high revenue leading to cereal-dominated crop rotations with increasing risk due to unfavourable preceding crops or preceding crop combinations. Based on a long-term field trial (1988–2001) with 15 different rotations including winter oilseed rape (OSR), winter wheat, winter barley, spring peas and spring oats, the effects of different preceding crops, pre-preceding crops and crop rotations on the grain yield of mainly OSR, winter wheat and winter barley were quantified. In the subsequent 2 years (2001/2002 and 2002/2003), winter wheat was grown on all plots in order to test the residual effects of the former crops (as preceding crops in 2002 and as pre-preceding crops in 2003) and crop rotations on growth, grain yield and yield components.

Unfavourable preceding crops significantly decreased yield of OSR, wheat and barley by 10% on average, however, with a large year-to-year variation. In addition, break-crop benefits in both crops, wheat and OSR, persisted to the second year. Wheat as preceding crop mainly decreased the thousand grain weight, and to a lesser extent, the ear density of the subsequent wheat crop. The amount of wheat yield decrease negatively correlated with the simple water balance (rainfall minus evapotranspiration) in May–July. In 2001/2002 and 2002/2003, the preceding crop superimposed the crop rotation effects, thus resulting in similar effects as observed in 1988–2001.

Our results clearly reveal the importance of a favourable preceding crop for the yield performance of a crop, especially wheat and OSR.     

Long continued applications of N fertilizer to cereals on sandy loam: grain and straw response to residual N

Abstract. The residual value of mineral N fertilizer applied in the spring was investigated in a field experiment where four cereals (winter wheat, winter barley, spring barley and spring oats) had been grown at reduced (0.7N), normal (1N) or high (1.3N) N fertilizer rates for 20 to 28 years. The effect of previous N fertilizer dressing was tested in two succeeding years by replacing the original N rate with five test N rates ranging from 0 to 240 kg N ha^?1 for winter cereals and 0 to 200 kg N ha^?1 for spring cereals. In the first test year, winter wheat grown on plots previously supplied with the high rate of mineral fertilizer (202 kg N ha^?1 yr^?1) yielded more grain and straw and had a higher total N uptake than wheat on plots previously supplied with the normal (174 kg N ha^?1 yr^?1) or reduced (124 kg N ha^?1 yr^?1) rate. The grain yield response and N uptake was not significantly affected by the N supply in the test year. The winter wheat grown in the second test year was unaffected by the previous N supply. Grain and straw yield response and total N uptake for spring barley, winter barley and oats, were almost identical irrespective of the previous N rate. After 20 to 28 years there were no significant differences in soil C and N (0 to 20 cm) between soil receiving three rates of N fertilizer. Soil from differently fertilized oat plots showed no significant differences in N mineralizing capacity. Nitrate leaching losses from the soils at the three N rates were estimated and the N balances for the 20 to 28 years experimental period calculated. The data indicated a reduction in overall loss of 189 to 466 kg N ha^?1 at the normal and high N rates compared with the reduced N rate. We conclude that the N supplying capacity and soil organic matter content of this fertile sandy loam soil under continuous cereal cropping with straw removal was not significantly affected by differences in N fertilizer residues.     

Ermittlung von Kennwerten optimaler Magnesium-Versorgung für Hafer,Sommerweizen und Mais

Determination of plant analysis criteria characterizing the magnesium nutrition of oats, spring wheat and maize Cereal plants from pot trials were analyzed for criteria of optimal Mg nutrition. The critical Mg concentration in dry matter of aerial parts from oats and spring wheat at the beginning of the shooting stage was found to be 1.8 and 1.3 per mille Mg respectively. The critical Mg concentration with maize is about 2,2 per mille in dry matter of yount leaves at tasseling stage. Because the Mg content of oats (based on dry matter) decreases significantly during the shooting stage, it seems advisable to have additional data (Mg content of green matter, Mg/N ratio, Mg/Ca ratio) which are less influenced by plant age. The Mg/Ca ratio appears to be well suited for evaluating the nutritional status of cereals. Minimum critical Mg/Ca ratios (based on per mille contents) are 0,23 for spring wheat, 0.3 for oats and 0.4 for maize.     

Leaching and plant offtake of N in field pea/cereal cropping sequences with incorporation of 15N-labelled pea harvest residues

Abstract. Field peas (Pisum sativum L.) were grown in sequence with winter wheat (Triticum aestivum L.) or spring barley (Hordeum vulgare L.) in large outdoor lysimeters. The pea crop was harvested either in a green immature state or at physiological maturity and residues returned to the lysimeters after pea harvest. After harvest of the pea crop in 1993, pea crop residues (pods and straw) were replaced with corresponding amounts of ¹⁵N‐labelled pea residues grown in an adjacent field plot. Reference lysimeters grew sequences of cereals (spring barley/spring barley and spring barley/winter wheat) with the straw removed. Leaching and crop offtake of ¹⁵N and total N were measured for the following two years. These treatments were tested on two soils: a coarse sand and a sandy loam. Nitrate concentrations were greatest in percolate from lysimeters with immature peas. Peas harvested at maturity also raised the nitrate concentrations above those recorded for continuous cereal growing. The cumulative nitrate loss was 9–12 g NO₃‐N m^–2 after immature peas and 5–7 g NO₃‐N m^–2 after mature peas. Autumn sown winter wheat did not significantly reduce leaching losses after field peas compared with spring sown barley. ¹⁵N derived from above‐ground pea residues accounted for 18–25% of the total nitrate leaching losses after immature peas and 12–17% after mature peas. When compared with leaching losses from the cereals, the extra leaching loss of N from roots and rhizodeposits of mature peas were estimated to be similar to losses of ¹⁵N from the above‐ground pea residues. Only winter wheat yield on the coarse sand was increased by a previous crop of peas compared to wheat following barley. Differences between barley grown after peas and after barley were not statistically significant. ¹⁵N lost by leaching in the first winter after incorporation accounted for 11–19% of ¹⁵N applied in immature pea residues and 10–15% of ¹⁵N in mature residues. Another 2–5% were lost in the second winter. The ¹⁵N recovery in the two crops succeeding the peas was 3–6% in the first crop and 1–3% in the second crop. The winter wheat did not significantly improve the utilization of ¹⁵N from the pea residues compared with spring barley.     

Macronutrient variation in small grain forage as related to specie and variety

Abstract

Incidence of grass tetany on small grain pastures has been related to forage Mg content and K/Ca + Mg ratio. The objective of this study was to relate P, K, Ca, Mg, and the K/Ca + Mg ratio in winter forage to specie and variety. In one year on unlimed soil with low pH, rye forage tended to be higher in P than oats, barley, or wheat. P content increased the next year on higher pH soil with less specie differences. K differed little with specie, and was higher in November than later harvests. Rye tended to be higher in Ca both years, especially in the early harvest. Percent Mg was lower for wheat the first year on the low pH soil than the other species, and percent Mg increased in all species at all harvests the next year on higher pH soil with wheat having similar Mg levels to the other species. The K/Ca + Mg ratio of wheat was higher than rye, oats, and barley on the low pH soil. Liming reduced this ratio to near 2.2 on all species. Grass tetany has been reported more likely to occur when K/Ca + Mg is over 2.2, and this study suggests rye, followed closely by oats and barley, would maintain lower ratios than wheat under conditions of low Mg availability.     

Stickstoffbilanz von 15N-Harnstoff bzw. 15N-Ammonsulfatsalpeter mit Dicyandiamid in Gefäßversuchen zu Grünhafer und Sommerweizen

Recovery of Nitrogen of ¹⁵N-urea and ¹⁵N-ammonium sulfa-nitrate with addition of dicyandiamide in pot trials with green oats and spring wheat In pot trials with green oats and spring wheat, the effect of ¹⁵N-urea (UR) and ¹⁵N-ammonium sulfa-nitrate (ASN) with addition of the unlabelled nitrification inhibitor dicyandiamide (DCD) was investigated on the basis of equal amounts of N by fully taking into account the DCD-N, with respect to yield, N-removal, distribution of ¹⁵N in plants and soil (sandy loam, pH 6.5) as well as the fate of DCD.

• 1 In Neubauer experiments with green oats, 62–69% of fertilizer-N were found in the shoots, 21–31% in the roots and 4–9% in the soil. In Mitscherlich trials with spring wheat, 85–88% of fertilizer-N were found in grain and straw, 7–10% in the roots and 3–4% in the soil. ¹⁵N-recovery, in all cases was between 98-100%, thus exhibiting pratically no loss in course of the experiment.
• 2 Yields of green oats (shoots), resp. spring wheat (grain and straw) were about 4-11 % lower in pots with DCD; on the contrary, N-contents and removal were somewhat higher compared to control without DCD.
• 3 With conditions given by these pot trials (strong root penetration, favourable temperature, repeated supply during early growth, slow soil-specific decomposition), water-soluble DCD was taken up partly by the plants and appeared mainly in leaves resp. straw. In grains of spring wheat, DCD-contents were, however, very low (maximum 3 ppm DCD-N); in roots it could not be detected. In comparison, analysis of crop-material in field trials showed no DCD in grains and only very few ppm DCD in straw.
• 4 In pots with DCD, the residual-N in the soil was higher, probably because of the prolonged time of reaction between ammonium and the organic matter of the soil.

     

Effect of drought on yield variability of key crops in Czech Republic

The relationship between seasonal agricultural drought and detrended yields (within a period from 1961 to 2000) of selected crops was assessed in the conditions of the Czech Republic, which are to some extent representative of a wider area of Central Europe. Impact of water stress was analyzed using time series of yields for 8 crops (spring barley, winter wheat, grain maize, potato, winter rape, oats, winter rye and hay from permanent meadows) for 77 districts in the Czech Republic (average district area is 1025 km²). Relative version of Palmer’s Z-index (rZ-index or rZ-i) was used as a tool for quantification of agricultural drought. The monthly values of the rZ-index for each individual district were calculated as the spatial average (only for the grids of arable land). The study showed that severe droughts (e.g., in 1981 and 2000) are linked with significant reduction in yields of the main cereals and majority of other crops through the most drought prone regions. We found a statistically significant correlation (p ≤ 0.05) between the sum of the rZ-index for the main growing period of each crop and the yield departures of spring barley within 81% (winter wheat in 57%, maize in 48%, potato in 89%, oats in 79%, winter rye in 52%, rape in 39%, hay in 79%) of the analyzed districts. This study also defined the crop-specific thresholds under which a soil moisture deficit (expressed in terms of rZ-index) leads to severe impact at the district level. This can be expressed as the sum of the monthly rZ-index during the period of high crop sensitivity to drought; for spring barley it is ?5, winter wheat ?5, maize ?9, rape ?12, winter rye ?10, oat ?4, potato ?6 and for hay ?3. The length of the sensitive period is also crop-specific and includes the months that are important for the yield formation. The results show that yields of spring barley (and spring crops in general) are significantly more affected by seasonal water stress than yields of winter crops and hay from permanent meadows. The study proved that a severe drought spell during the sensitive period of vegetative season does have a quantifiable negative effect, even within more humid regions. These results demonstrate that, at least in some areas of the CR (and probably most of Central Europe), drought is one of the key causes of interannual yield variability.     

The Occurrence of Type A and B Trichothecenes in Lithuanian Cereals

Samples of winter wheat (n =84), winter rye (46) and barley (29) were collected from the larger family farms and from partnerships in Lithuania just after the 1998 harvest. The number of samples collected from each region was proportional to the amount of grain produced in it. The levels of the Fusarium toxins deoxynivalenol (DON), 3-acetyl-DON, 15-acetyl-DON, nivalenol (NIV), fusarenon-X (4-acetyl-NIV), T-2 toxin, HT-2 toxin, 4,5-diacetoxyscirpenol (DAS), 1,5-monoacetoxyscirpenol (MAS) and scirpentriol in the grain were determined by gas chromatography with mass-selective detection (GC-MS). DON was most often detected in the wheat and rye samples and NIV in the barley samples. The concentrations found were lower than those causing acute or chronic toxic effects in livestock or humans. No fusarenon-X or 15-acetyl-DON was detected, and only small amounts of other trichothecenes were present. Climatic conditions in Lithuania in the summer of 1998 were slightly cooler and wetter than the average for the 1992-1996 but were close to the norm. Because the samples analysed were representative of grain produced for the market in seasons with normal weather, trichothecene contamination of grain from large family farms and partnerships would not be expected to be a problem in most years.     

Estimation of the Deoxynivalenol and Moisture Contents of Bulk Wheat Grain Samples by FT‐NIR Spectroscopy

Deoxynivalenol (DON) levels in harvested grain samples are used to evaluate the Fusarium head blight (FHB) resistance of wheat cultivars and breeding lines. Fourier transform near‐infrared (FT‐NIR) calibrations were developed to estimate the DON level and moisture content (MC) of bulk wheat grain samples harvested from FHB screening trials. Grains in a rotating glass petri dish were scanned in the 10,000–4,000 cm⁻¹ (1,000–2,500 nm) spectral range using a Perkin Elmer Spectrum 400 FT‐IR/FT‐NIR spectrometer. The DON calibration predicted the DON levels in test samples with R ² = 0.62 and root mean square error of prediction (RMSEP) = 8.01 ppm. When 5–25 ppm of DON was used as the cut‐off to classify samples into low‐ and high‐DON groups, 60.8–82.3% of the low‐DON samples were correctly classified, whereas the classification accuracy of the high‐DON group was 82.3–94.0%. The MC calibration predicted the MC in grain samples with R ² = 0.98 and RMSEP = 0.19%. Therefore, these FT‐NIR calibrations can be used to rapidly prescreen wheat lines to identify low‐DON lines for further evaluation using standard laboratory methods, thereby reducing the time and costs of analyzing samples from FHB screening trials.     

Importance of soil mineral N in early spring and subsequent net N mineralisation for winter wheat following winter oilseed rape and peas in a milder climate

Abstract

Nine biennial field experiments, 2000–2004, in south Sweden, 55–56°N, with winter wheat following winter oilseed rape, peas, and oats, were used to estimate the impact of a future milder climate on winter wheat production in central Sweden, 58–60°N. The trials included studies 1) on losses during winter of soil mineral nitrogen (N_min, 0–90 cm soil), accumulated after the preceding crops in late autumn, 2) on soil N mineralisation (N_net) during the growing season of the wheat (early spring to ripeness) and 3) on grain yield and optimum N fertilisation (Opt-N rate) of the wheat. Average N_min in late autumn following winter oilseed rape, peas, and oats was 68, 64, and 45 kg ha^?1, respectively, but decreased until early spring. Increased future losses of N_min during the winter in central Sweden due to no or very short periods with soil frost should enhance the demand for fertiliser N and reduce the better residual N effect of winter oilseed rape and peas, compared with oats. Their better N effect will then mainly depend on larger N_net (from March to maturity during the winter wheat year). Owing to more plant-available soil N (mainly as N_net) Opt-N rates were lower after oilseed rape and peas than after oats despite increased wheat yields (700 kg ha^?1) at optimum N fertilisation. In addition to these break crop effects, a milder climate should increase winter wheat yields in central Sweden by 2000–3000 kg ha^?1 and require about 30–45 kg ha^?1 more fertiliser N at optimum N fertilisation than the present yield levels. Increased losses and higher N fertilisation to the subsequent winter wheat in future indicates a need for an estimation of the residual N effect at the individual sites, rather than using mean values as at present, to increase N efficiency.     

Cultivation of wheat following pea and triticale/pea mixtures increases yields and nitrogen content

Abstract

An experiment was conducted in 2004–2007 at the University of Podlasie Zawady Experimental Station (52°06′N, 22°50′E), Siedlce, Poland, to examine the effect of either post-harvest residues or residues and straw of spring triticale (Triticale), field pea (Pisum sativum L.) and their mixtures containing the following proportions of both components: 75+25, 50+50, 25+75% on the subsequent crop of winter wheat (Triticum aestivum L.). A field experiment was designed as split-blocks with three replicates. Residue mass, straw mass as well as N, P, K, Ca and Mg amounts were determined in the residues and straw. The residue amount of spring triticale was the greatest. N, Ca and Mg amounts in the residues of spring triticale/field pea mixtures were similar or higher whereas P and K amounts were similar or lower compared with spring triticale residues. Spring triticale straw contained less N, P, Ca and Mg than the straw of either field pea or spring triticale/field pea mixtures. Grain yield, yield components, N content and N uptake in the grain of winter wheat following field pea and spring triticale/field pea mixtures were significantly higher compared with winter wheat following spring triticale. Increasing proportions of field pea in mixtures with spring triticale cultivated as previous crops significantly increased winter wheat grain yields as well as N content and uptake. The residues of the previous crops combined with the straw significantly increased winter wheat grain yield, number of ears per m², number of grains in an ear, thousand-grain weight and N content and uptake. The highest winter wheat grain yield and N uptake were determined following an application of residues and straw of field pea and 25+75% spring triticale/field pea mixture. The grain of winter wheat after field pea had the greatest N content.     

A Comprehensive Genotype and Environment Assessment of Wheat Grain Ash Content in Oregon and Washington: Analysis of Variation

A comprehensive analysis of the variation in wheat grain ash content has not been previously conducted. This study assessed the relative contribution of genotype and environment to variation in ash content, with a particular aim of ascertaining the potential for manipulating the trait using contemporary adapted germplasm. A total of 2,240 samples were drawn from four years of multilocation field plots grown in the wheat production areas of Oregon and Washington states. Genotypes included commercial cultivars and advanced breeding lines of soft and hard winter, and soft and hard spring wheats with red and white kernel color, several soft white club wheats, and one soft white spring waxy wheat cultivar. In addition to ash, protein content, test weight, and Single Kernel Characterization System kernel hardness, weight and size were also measured. In total, 20 separate fully balanced ANOVA results were conducted. Whole model R² values were highly significant, 0.62–0.91. Nineteen of the 20 ANOVA results indicated significant genotype effects, but the effects were not large. In contrast, environment effects were always highly significant with F values often one to two orders of magnitude larger than the genotype F values. The grand mean for all samples was 1.368% ash. For individual data sets, genotype means across environments varied ≈0.1–0.3% ash. The genotypes judged noteworthy because they had the highest least squares mean ash content were OR9900553 and ClearFirst soft white winter, NuHills hard red winter, Waxy‐Pen and Cataldo soft white spring, and WA8010 and Lochsa hard spring wheats. Genotypes with lowest least squares mean ash were Edwin (club) soft white winter, OR2040073H hard red winter, WA7952 soft white spring, and WA8038 hard spring wheats. In conclusion, wheat grain ash is more greatly influenced by crop year and location than by genotype. However, sufficient genotype variation is present to plausibly manipulate this grain trait through traditional plant breeding.     

A long-term comparison of ploughing, tine cultivation and direct drilling on the growth and yield of winter cereals and oilseed rape on clayey and silty soils

The effects of mouldboard ploughing, shallow tined cultivation and direct drilling on yields of winter wheat, barley, oats and oilseed rape were compared over 10 years. Three field experiments were conducted on two non-calcareous clays (stagnogleys) and a weakly structured silty soil (argillic brown earth). Two spring N levels were applied to the winter wheat plots on the clay soil in three years and to the winter barley plots on the silty soil in one year. This paper reports the soil bulk density and water content at sowing and the crop growth, yield components and yields obtained during the later years of the study: 1979–1984 on the clayey soils and 1981–1984 on the silty soil.

In the years when cereals were grown, differences in yield between cultivation treatments were small and inconsistent. Oilseed rape yielded significantly more after direct drilling than ploughing because of better establishment and uniformity of growth.

The success of continuous reduced tillage depended on both burning crop residues and good weed control.