A Marteilia-like parasite in blue mussels Mytilus edulis in China

Species of the genus Marteilia (Phylum Paramyxea) are protozoan parasites of marine mollusks. Marteilia spp. have been detected in mollusks from different parts of the world, but the presence of these parasites in China has not been previously reported. Therefore, a survey was conducted to look for the presence of Marteilia spp. in blue mussels Mytilus edulis and Asian green mussels Perna viridis collected along China's coasts. Histological and PCR analyses revealed that 5 of 180 M. edulis (prevalence = 2.8%) were positive for infection with a Marteilia-like organism, whereas the parasite was not detected in any of the 80 P. viridis individuals tested. Total genomic DNA was extracted from the infected tissue sections for PCR amplification. The PCR amplification with Marteilia primers SS1 and SAS1 yielded the expected 641-bp product. Sequencing results showed that the 18S ribosomal RNA gene fragment from the protozoans found in M. edulis from China was 88% similar to that of Marteilia refringens, a species that was reported from M. edulis and European flat oysters Ostrea edulis collected in France. This is the first report of a Marteilia-like organism infecting M. edulis in China.     

Experimental Encephalitozoonosis in the Blue Fox: Neonatal Exposure to the Parasite

Newborn and young pups up to the age of 15 days were exposed to E. cuniculi, either by keeping the pups in cages together with orally inoculated foster-mothers and their offspring, or by oral inoculation with E. cuniculi spores. A majority of pups appeared sero-positive to E. cuniculi with the india-ink immuno-reaction from 35 to 87 days post exposure; spores of E. cuniculi were detected in organs of some of the animals. The non-inoculated pups kept together with the orally inoculated pups became seropositive from 49 to 129 days after the oral inoculations. However, the exposure of newborn and young pups failed to induce clinical encephalitozoo-nosis, and when killed at the time of pelting the body weights and fur quality appeared to be within the normal range in all exposed foxes. No macroscopic lesions were detected in the various organs. Histologically focal interstitial nephritis occurred in the great majority of the seropositive animals. Meningoencephalitis was seen in some of the foxes, whereas slightly thickened walls of some arteries, mainly in the myocardium, were found in a few animals. The lesions of the brain and kidneys seem to be very similar to those seen in chronic cases of rabbit encephalitozoonosis. Polyarteritis nodosa and severe encephalitis and interstitial nephritis with extensive proliferations of plasma cells, which are almost constant findings in cases of clinically diseased foxes, were not detected in any of the subclinically infected animals. Various factors that might be of significance in the pathogenesis of the disease are discussed, and it is concluded that intrauterine infection of the pups via the transplacental route appears to be an essential supposition for the establishment of clinical fox encephalitozoonosis.     

Experimental Encephalitozoonosis in the Blue Fox: Transplacental Transmission of the Parasite

Spores of Encephalitozoon cuniculi were recovered from foetal and placental tissues from blue fox females orally inoculated with the parasite. The results provided evidence for transplacental transmission of the causative agent of fox encephalitozoonosis.     

中国蓝狐种源问题的探讨

介绍了中国蓝狐的养殖历史与发展现状,并由此发现在国内饲养的蓝狐种群中出现了严重的种源退化问题.探讨了出现品种退化原因,并针对蓝狐品种品质退化的问题提出了一些相应的解决办法和措施.     

Parasite transmission in confined hogs

Examinations of fecal specimens from swine of all ages maintained in a totally enclosed confinement facility in south Georgia were conducted in a 5-year survey (1977-1981) to determine the prevalence, intensity, and transmission patterns of intestinal nematode and protozoan parasites. Weaned pigs in the nursery had no detectable parasitic infections except sporadic Isospora suis in newly weaned pigs. Growing-finishing hogs had a low prevalence of Ascaris suum, Oesophagostomum spp. and Balantidium suis. Mean A. suum prevalence and mean eggs per gram feces (EPG) increased until about 200 days of age and then declined. Oesophagostomum spp. and B. suis prevalence and intensity tended to increase throughout life. Gilts (mean age 273 days) in the gestation unit had a higher (P less than 0.01) prevalence of A. suum and lower (P less than 0.01) prevalences of Oesophagostomum spp. and B. suis than sows (mean age 706 days). Similarly, A. suum EPG was higher (P less than 0.01) and Oesophagostomum spp. EPG and B. suis cysts per gram feces (CPG) were lower (P less than 0.01) in gilts than in sows. No evidence for a peri-parturient increase in parasite eggs or cysts was found. Instead, there was a higher prevalence of B. suis (P less than 0.01) in gestating than in lactating animals and more lactating sows than gestating sows were negative for parasites (P less than 0.01). Apparent differences due to location (gestation unit or farrowing unit) were largely attributable to age differences. Trichuris suis infections were rare and not shown to be established in the herd. Strongyloides ransomi in suckling piglets was controlled by anthelmintic therapy. I. suis occurred in piglets throughout the study but was never found in sows in the farrowing unit, either before or after birth of a subsequently infected litter of piglets. No parasites requiring intermediate hosts occurred.     

Parasite Infections in Danish Trout Farms

Samples from 5 Danish freshwater trout farms rearing rainbow trout (Oncorhynchus my kiss) were examined for parasite infections from October 1993 until November 1994 and recorded parasites are listed. In addition, results from an examination of a mariculture net cage system are presented as well. A total of 10 metazoan and 10 protozoan parasites were recorded. The metazoans included Gyrodactylus derjavini, Gyrodactylus salaris, Eubothrium crassum, Triaenophorus nodulosus, Proteocephalus sp., Diplostomum spathaceum, Tylodelphus clavata and Argulus foliaceus from the freshwater farms. The protozoans Hexamita salmonis, Ichthyobodo necator, Ichthyophthirius multifiliis, Apiosoma sp., Epistylis sp., Trichodina nigra, T. mutabilis, T. fultoni, Trichodinella epizootica, and an Ichthyophonus like intestinal parasite were also detected in the freshwater trout farms. Based on lectin binding studies, few fish were found positive for the myxosporean parasite PKX although no clinical cases were reported. In the mariculture system, Lepeophtheirus salmonis and Caligus elongatus were found.     

Parasite infections in laboratory mice colonies

Problems dealing with common parasites--oxyurids, which affect laboratory mice colonies are discussed and reviewed. Their life histories, pathogenicity and immunity are examined. The attention is paid to the influence of age, sex, strain and status of the host in the infection. It is agreed that helminth infections are usually more severe in male than in female vertebrate hosts.     

藏羚羊寄生虫感染现状分析

藏羚羊又称藏羚、长角羊等,为国家一级保护动物,主要分布在中国青藏高原(西藏、青海和新疆),被称为"可可西里的骄傲",也是列入《濒危野生动植物种国际贸易公约》中严禁贸易的濒危动物。关于藏羚羊迁徙的文章有很多,但有关它的疾病方面的研究很少,寄生虫病研究也不多。迄今报道的藏羚羊体内、外共发现7种寄生虫,其中原虫1种、线虫2种、绦虫2种、体外寄生虫2种。文中对其寄生虫感染的情况及原因作一综述,为野生动物寄生虫病的防治提供参考。     

云南省猪寄生虫区系调查

随机抽样对云南省18个地州78个、县市不同自然环境饲养的猪,按蠕虫学完全解剖法收集到虫体28680条,逐条鉴定,并用IHA对2731头猪检查原虫感染精况。共鉴定出虫体58种,隶属于8纲、17目、28科、36属。其中吸虫8种,绦虫6种,线虫25种,棘头虫1种,蜘蛛昆虫5种,原虫13种。     

云南省猪寄生虫区系调查

随机抽样对云南省18个地州78个、县市不同自然环境饲养的猪,按蠕虫学完全解剖法收集到虫体28 680条,逐条鉴定,并用IHA对2 731头猪检查原虫感染精况。共鉴定出虫体58种,隶属于8纲、17目、28科、36属。其中吸虫8种,绦虫6种,线虫25种,棘头虫1种, 蜘蛛昆虫5种,原虫13种。     

Parasite control in calves and growing heifers.

Parasite control in calves and growing heifers results in animals that grow faster and remain healthier. Control programs revolve around reducing the parasites (infective stages) in the environment. In confinement, sanitation removes or reduces the number of infective stages (oocysts, cysts, and infective larvae) and the infection rate. On pasture one must resort to pasture management and parasite removal. The mites and lice are generally controlled by reducing or removing the populations on the animals, preventing the spread. Environmental control and manure management impact the fly populations.     

DNA条形码技术及其在蚌类保护生物学中的研究

作者对DNA条形码的定义与特征、原理、操作步骤和应用范围等进行了介绍,概括了在物种鉴定中的研究现状,并阐述了该技术在蚌类保护生物学中的应用及前景。     

昆明市流浪犬寄生虫感染情况的调查

为了解昆明市流浪犬寄生虫感染情况,随机采集25份新鲜粪样进行饱和盐水漂浮法检查;对18只死亡流浪犬进行寄生虫解剖法检查,收集虫体,进行计数和鉴定.结果显示,14份粪样中检出虫卵或卵囊,检出率为56.0％.其中8份检测出球虫卵囊,检出率32.0％;6份检测出犬弓首蛔虫卵,检出率24.0％,2份检测出绦虫卵,检出率8.0％...