非淀粉多糖酶对仔猪早期生产性能和消化性能的影响

选用10头母猪生产的100多头仔猪 ,研究了玉米—豆粕型仔猪日粮中添加非淀粉多糖酶对仔猪早期生长性能和消化性能的影响。试验组在对照组日粮中添加120g/t非淀粉多糖酶。试验结果表明 ,添加非淀粉多糖酶使断奶仔猪蛋白质消化率提高9.67 %(P<0.05) ,有机物消化率提高15.40 %(P<0.05) ;添加非淀粉多糖酶对仔猪断奶前的生长无明显影响 ,但对断奶后0～14天的生长有显著的促进作用 ,可提高仔猪日增重10.40 %(P<0.05) ,提高窝重17.65 %(P<0.05)。试验结果同时提示断奶仔猪日粮中添加非淀粉多糖酶可有效地缓解仔猪断奶时的应激反应     

Ileal digestibility of protein and amino acids from canola meal in weaned piglets and growing pigs

Two experiments were conducted to measure the apparent ileal digestibility coefficients (AID) of protein and amino acids from canola meal (CM) and a pelleted canola meal (PCM) and their effect on specific activity (SA) of pancreatic proteases in weaned piglets and growing pigs. In experiment one, 24 piglets weaned at 17 days and weighing 5.5 kg were used. Treatments were a reference diet with 200 g of crude protein (CP) kg^− 1 elaborated with casein (C) as the sole protein source, a diet containing C–CM and a diet containing C–PCM. These diets were obtained using the reference diet plus 100 g kg^− 1 of CM or PCM that substituted an isoproteic mixture of casein and maize starch from reference diet, so that the AID coefficients for their protein and amino acids could be calculated by difference. In experiment two, nine castrated pigs weighing 39.5 kg were used. Treatments were a reference diet with 160 g of CP kg^− 1 elaborated with casein (C) as the sole protein source, diet C–CM and diet C–PCM. These diets were obtained using the reference diet plus 300 g kg^− 1 of CM or PCM that substituted an isoproteic mixture of casein and maize starch from reference diet. In piglets, the AID coefficients for casein were highest (P < 0.05), those of PCM were intermediate, and those of CM were the lowest. In older pigs, the AID coefficients for casein were highest, and those of CM and PCM were similar (P > 0.05). The SAs of chymotrypsin, trypsin and carboxypeptidases A and B were lower in piglets than in heavier pigs. Moreover, the SAs of trypsin, chymotrypsin, and carboxypeptidase B were lower (P > 0.05) in animals fed casein. The results showed in piglets that whereas CM was less digestible, pelleted canola meal improved protein and amino acid ileal digestibility, resulting in similar AID coefficients to those of growing pigs.     

Glutamic acid supplementation recovers the reduced performance of weanling pigs fed reduced crude protein diets

This study was conducted to evaluate the supplementation of glutamic acid(Glu)to reduced protein diets on the performance of weanling pigs.One hundred and eighty crossbred weanling pigs([Yorkshire
Landrace]
Duroc,21 d old)having similar body weight(BW)of 6.45 kg were randomly allotted to 1 of 6 dietary treatments(5 pigs per pen[2 barrows and 3 gilts];6 pens per treatment)based on BW and sex during a 6-week trial.Dietary treatments consisted of positive control(PC)diet formulated to have 226.9,205.6,and 188.8 g crude protein(CP)during phases 1,2,and 3,respectively,and negative control(NC)diets with 20 g CP reduction from PC diets and addition of Glu with increasing levels,resulting in the calculated Lys-to-Glu ratios of 1:2.25,1:2.30.1:2.35,1:2.40,and 1:2.45,designated as NC,NC1,NC2,NC3,and NC4,respectively.The BW of pigs receiving PC diet was higher(P<0.05)than those receiving NC diet at d 7,21 and 42.A higher(P<0.05)average daily gain(ADG)from d 1 to 7,8 to 21,22 to 42 and during the overall experiment period was observed in pigs fed PC than NC diet.Pigs fed NC diets including the graded level of Glu linearly increased(P<0.05)BW at d 42,ADG and gain-to-feed ratio(G:F)during the overall experimental period.In addition,trends in linear increase in BW(P=0.056)at d 7 and ADG from d 1 to 7 and d 22 to 42(linear effect,P=0.081,P=0.058 respectively)were observed.A tendency in the linear increment of NH3(P=0.082)at d 21 and linear reduction in methyl mercaptans(P=0.054)emission at d 42 was observed in pigs fed NC diets supplemented with graded level of Glu.In conclusion,supplementing the reduced protein diet with Glu enhanced the growth performance in weanling pigs suggesting that supplementation of Glu can compensate the reduction of 2%CP in the basal diets.     

Changes in diarrhea,nutrients apparent digestibility,digestive enzyme activities of weaned piglets in response to chitosan‐zinc chelate

A total of 120 weanling barrows weighing 6.11 ± 0.20 kg were randomly allotted to four treatments with three replications (i.e. pen) of ten piglets per replicate. Pigs were received corn‐soybean basal diet (control) or the same basal diet supplemented with the following sources of zinc: (i) 100 mg/kg of Zn as ZnSO₄; (ii) 100 mg/kg of Zn as chitosan‐Zn chelate (CS‐Zn); and (iii) 100 mg/kg of Zn as ZnSO₄ mixed with chitosan (CS + ZnSO₄). The results showed that CS‐Zn could highly improve average daily gain and average daily feed intake than those of ZnSO₄ or CS+ ZnSO₄ (P < 0.05). The pigs fed dietary CS‐Zn had lower diarrhea incidence and higher apparent digestibility of crude protein than those of the pigs fed dietary ZnSO₄ (P < 0.05). The protease activities in duodenal content of the pigs receiving CS‐Zn diets was higher than that of the pigs fed dietary ZnSO₄ or CS + ZnSO₄ (P < 0.05). The amylase activity in duodenal content of the pigs fed dietary CS‐Zn was higher than that of the pigs receiving ZnSO₄ diets or basal diets (P < 0.05). These results indicated that dietary CS‐Zn showed different bioactivities from ZnSO₄ or CS + ZnSO₄ in reducing the incidence of diarrhea, improving activities of digestive enzymes and growth performance of weaned pigs.     

Influence of Bacillus subtilis GCB‐13‐001 on growth performance,nutrient digestibility,blood characteristics,faecal microbiota and faecal score in weanling pigs

The present study investigated the influence of Bacillus subtilis GCB‐13‐001 on growth performance, nutrient digestibility, blood characteristics, faecal microbiota and faecal score in weanling pigs. A total of 120 weaning pigs [(Landrace × Yorkshire) × Duroc; 7.73 ± 0.75 kg (28 days of age)] were randomly allotted into three treatments according to their initial body weight (BW) and gender in a 6‐week experiment. There were 8 replication pens in each treatment, with five pigs/pen. Dietary treatment groups were as follows: (a) basal diet (CON), (b) CON + 0.1% Bacillus subtilis GCB‐13‐001 1 × 10⁸ CFU/kg (T1) and (c) CON + 0.1% Bacillus subtilis GCB‐13‐001 1 × 10⁹ CFU/kg (T2). Days 1 to 7, the BW and ADG with T2 treatment were higher (p < .05) than CON treatment, as well as F:G showed trends in linear reduction (p < .1). Days 8 to 21, the BW and ADG were improved (p < .05) in pigs offered T1 and T2 diets compared with CON diet. Days 22 to 42, BW and ADG were higher (p < .05) in pigs fed T2 diet than CON and T1 diets, and the pigs fed T1 diet had higher BW than CON treatment. Overall, the ADG with the T2 treatment was higher (p < .05) than that with the T1 and CON treatments, and pigs offered T1 treatment had higher (p < .05) ADG than CON treatment. Moreover, F:G ratio were significantly decreased (p < .05) by T2 treatment compared with CON treatment. The faecal Lactobacillus counts were improved, and E. coli counts were reduced (p < .05) in pigs fed T2 diet compared with CON at the end of the experiment. In conclusion, supplementation of 0.1% Bacillus subtilis GCB‐13‐001 1 × 10⁹ CFU/kg has shown a beneficial effect in improving BW, increase ADG, decrease F:G ratio.     

Effects of dietary supplementation of fermented garlic powder on growth performance,apparent total tract digestibility,blood characteristics and faecal microbial concentration in weanling pigs

A total of 144 weanling pigs (5.5 ± 0.42 kg) were used in a 5‐week trial to evaluate the effect of fermented garlic powder (FGP) supplementation in weaning pigs. Pigs were randomly allocated to the following four dietary treatments: (i) control (CON; basal diet), (ii) FGP0.5 (CON + 0.5 g/kg FGP), (iii) FGP1 (CON + 1 g/kg FGP), (iv) FGP2 (CON + 2 g/kg FGP). There were six replications with six pigs (three barrows and three gilts) per pen. Pigs fed FGP0.5 treatments increased (p < 0.05) average daily gain (ADG) and average daily feed intake (ADFI) during 3–5 and 0–5 week. No difference was observed on gain/ feed (G/F) ratio in this study. The inclusion of FGP1 and FGP2 treatments increased DM (p < 0.05) digestibility compared with the CON treatment throughout the experiment. The apparent total tract digestibility of N was (p < 0.05) increased by the dietary FGP throughout the experiment. Pigs fed FGP1 and FGP2 treatments increased the lymphocyte concentration (p < 0.05) and RBC concentration compared with the CON group. Dietary FGP decreased the blood total cholesterol (p < 0.05) in comparison with the CON group. The triglyceride (p < 0.05) concentration was decreased in FGP1 and FGP2 treatments compared with those in the CON treatment at the end of the experiment. Pigs fed FGP1 and FGP2 treatments had a lower number of Escherichia coli in faeces on 21 (p < 0.05) and 35 days (p = 0.05) than those in the CON treatment. In conclusion, dietary fermented garlic powder (0.5 g/kg) can improve ADG and ADFI in weanling pigs, but not at higher levels (1 and 2 g/kg). Dietary fermented garlic powder can also increase the nutrient digestibility, lymphocytes and RBC concentrations, but decrease the faecal E. coli concentration in weaning pigs.     

Effect of salmon protein hydrolysate and spray-dried plasma protein on growth performance of weanling pigs

Two experiments, each consisting of 2 trials, were conducted to determine the effect of salmon protein hydrolysate (SPH) and spray-dried plasma protein (SDPP) fed during the first week postweaning and their subsequent effect on the growth performance of weanling pigs. Pigs were fed in a 3-phase feeding program with durations of 7 d for phase 1 in both Exp. 1 and 2; 14 or 15 d for phase 2 in Exp. 1 and 2, respectively; and 7 or 8 d for phase 3 in Exp. 1 and 2, respectively. Dietary treatments were fed only during phase 1, whereas the same diet was fed to all pigs in phases 2 and 3. Pigs were blocked by initial BW and sex, and littermates were balanced across treatments. Data from the 2 trials within each experiment were combined and analyzed together; no treatment × trial interactions (P > 0.10) were observed. In Exp. 1, a total of 324 weanling pigs (10 replications of 5 or 6 pigs per pen) with an average initial BW of 6.4 ± 1.3 kg were assigned to 1) a control diet with no SPH or SDPP, 2) 1.5% SPH, 3) 3.0% SPH, 4) 1.5% SDPP, 5) 3.0% SDPP, or 6) 1.5% SPH + 1.5% SDPP. Experiment 2 was similar to Exp. 1, but red blood cells were removed from all diets to reduce diet complexity. In Exp. 2, weanling pigs (n = 320, 14 replications of 5 or 6 pigs per pen) with an average initial BW of 5.4 ± 1.2 kg were assigned to 1) a control diet with no SPH or SDPP, 2) 1.5% SPH, 3) 1.5% SDPP, or 4) 1.5% SPH + 1.5% SDPP. Three batches of SPH were used, and each batch was analyzed for AA composition. In Exp. 1, the inclusion of SDPP or SPH during phase 1 did not affect (P > 0.10) ADG, ADFI, or G:F compared with those of pigs fed the control diet. No carryover effects on growth performance were observed in any of the subsequent phases. Overall, G:F was greater (P = 0.08) in pigs fed the 1.5% diets compared with those fed the 3.0% diets. In Exp. 2, no differences (P > 0.10) were observed in ADG, ADFI, or G:F among pigs fed the SPH or SDPP diets compared with those of pigs fed the control diet. Pigs fed the combined diet had greater (P < 0.10) overall ADFI compared with that of pigs fed the control diet, but ADFI was similar to that of pigs fed the SPH and SDPP diets. These results indicate that inclusion of up to 3% SDPP or SPH in diets fed during the first week postweaning did not affect the growth performance of weanling pigs, and no subsequent carryover effects were observed. Salmon protein hydrolysate did not affect the growth performance of weanling pigs and may be considered an alternative protein source in diets for weanling pigs.     

Intake and digestibility in cattle fed low-quality tropical forage and supplemented with nitrogenous compounds

The effects of supplementation with nitrogenous compounds on intake, digestibility, and microbial protein synthesis in cattle fed low-quality tropical forage were assessed. Five rumen fistulated crossbred Holstein × Gir heifers were used, with initial average live weight of 180 ± 21 kg. Signal grass (Brachiaria decumbens) hay (48.6 g kg⁻¹ of crude protein (CP), on a dry matter (DM) basis) was used as roughage. Five treatments were defined according to nitrogen supplementation level (0, 20, 40, 60, and 80 g of CP kg⁻¹ above the CP level of the hay). A mixture of urea, ammonium sulfate, and albumin at the ratios of 4.5:0.5:1.0, respectively, was used as nitrogen source. The experiment consisted of five experimental periods, according to a 5 × 5 Latin square design. The average CP contents in the diets were 51.9, 71.1, 86.0, 116.7, and 130.2 g kg⁻¹, on a DM basis. A quadratic effect was detected (P < 0.10) of the CP levels in the diets on DM and neutral detergent fiber intake (kg/day), with maximum response at the levels of 102.4 and 100.5 g CP kg⁻¹ DM, respectively. The average daily concentration of rumen ammonia nitrogen showed increasing linear pattern (P < 0.01) as function of CP levels in the diet, with estimated value of 9.64 mg dL⁻¹ equivalent to the maximum DM intake. Microbial nitrogen flow in the intestine was linearly and positively related (P < 0.01) with the CP levels in the diet.     

Effects on performance of growing pigs fed diets containing different levels of dried cashew pulp

The purpose was to investigate the use of dried cashew pulp (DrCP), the sun-dried residue after juice has been extracted from the cashew apple, as a feed ingredient in growing pig diets. The growth rate, feed intake, feed to gain ratio and cost of gain of grower pigs fed diets containing 0 (0DrCP), 100 (100DrCP), 150 (150DrCP) or 200 (200DrCP) g kg⁻¹ of dried cashew pulp was investigated in a feeding trial set up as a Completely randomized design with 4 treatments replicated 3 times and lasting 112 days. There were no significant differences (P > 0.05) among treatments in the parameters measured. It was concluded that dried cashew pulp could be used in growing pig diets up to a level of 200 g kg⁻¹ without any deleterious effects. Future work will look at higher levels of DrCP inclusion in the diets of growing pigs.     

Effects of a whey protein product and spray-dried animal plasma on growth performance of weanling pigs

Five experiments were conducted to evaluate the effects of a high-protein, whey protein product (WPP; 73% CP, 6.8% lysine, 12.8% fat, and 5% lactose) and spray-dried animal plasma (SDAP) on growth performance of weanling pigs. In all experiments, pigs were fed experimental diets from d 0 to 14 after weaning in a pelleted form and then a common diet in meal form for the remainder of the experiment. Dietary treatments were established by substituting WPP or SDAP for dried skim milk (Exp. 1) or soybean meal (Exp. 2, 3, 4, and 5) in the control diet. In Exp. 1, we maintained a constant level of lactose in all diets by adjusting the amount of added crystalline lactose. The amount of lactose in diets used in Exp. 2 through 5 varied slightly by the addition of WPP. In Exp. 1 and 2, 180 weanling pigs (initially 5.8 kg and 19 +/- 1 d of age or 5.5 kg and 17 +/- 1 d of age, respectively) were used. Treatment diets contained SDAP (2.5 and 5%) or WPP (2.7 and 5.4% in Exp.1, and 2.5 or 5.0% in Exp. 2). In Exp. 1, from d 0 to 7 after weaning, ADG and ADFI increased with increasing SDAP (linear, P < .01). No other treatment effects were observed during the d 0 to 14 period. In Exp. 2, from d 0 to 14 after weaning, ADG and G:F increased (linear, P < .04) with increasing SDAP or WWP. In Exp. 3, 305 weanling pigs (initially 4.1 kg and 12 +/- 1 d of age) were used. The control diet contained 2.5% SDAP. The experimental diets were similar to the control diet but contained an additional 2.5 or 5.0% SDAP or 2.5 or 5.0% WPP. From d 0 to 14 after weaning, ADG, ADFI, and G:F increased (quadratic, P < .05) with increasing SDAP up to 5.0%. Increasing WPP increased ADG (quadratic, P < .07) and ADFI (linear, P < .09). In Exp. 4 and 5, 329 and 756 weanling pigs (initially 4.1 kg and 12 +/- 1 d of age and 5.2 kg and 18 +/- 1 d of age, respectively) were fed diets in which WPP was substituted for 0, 25, 50, 75, and 100% (Exp. 4) or 0, 50, and 100% (Exp. 5) of the SDAP in the control diet. In Exp. 4 and 5, from d 0 to 14 after weaning, pigs fed a 1:1 blend of each protein source had better ADG (quadratic, P < .04) than those only fed SDAP. In conclusion, WPP can be used in combination with or as a total replacement for SDAP in diets for weanling pigs without reducing performance.     

Effects of pelleting and pellet conditioning temperatures on weanling pig performance

We conducted two experiments to study the effects of pelleting and pellet conditioning temperature on weanling pig performance. In Exp. 1, 252 weanling pigs (PIC, L326 x C22) averaging 6.0 +/- 1.3 kg and 21 +/- 3 d of age were used to evaluate six corn-soybean meal-based diets containing 15% dried whey and formulated to contain 1.4% lysine. Treatments consisted of a control diet without spray-dried animal protein (SDAP) fed in meal form, a diet with 5% SDAP fed in meal form, and four diets with 5% SDAP that were conditioned at 60, 66, 71, or 77 degrees C for 10 s prior to pelleting. Pellets had a 3.97-mm diameter. The experimental diets were fed from d 0 to 14 after weaning, and all pigs were fed a common diet in meal form from d 14 to 28 after weaning. From d 0 to 7 after weaning, pigs fed diets containing SDAP had greater ADG, gain/feed (P < 0.001), and ADFI (P < 0.05) than pigs fed the control diet. No differences (P > 0.10) were observed between pigs fed the pelleted diets and those fed the SDAP diet in meal form. Conditioning temperature had no effect (P > 0.10) on weanling pig performance from d 0 to 14, and the diet fed from d 0 to 14 had no effect on overall performance (d 0 to 28). In Exp. 2, 252 weanling pigs (6.3 +/- 1.5 kg and 22 +/- 4 d of age) were used to evaluate diets with same composition as in Exp. 1, but treatments consisted of diets with or without SDAP conditioned at 60 degrees C before pelleting, and four diets containing 5% SDAP that were conditioned at 68, 77, 85, and 93 degrees C before pelleting. As in Exp. 1, conditioning lasted 10 s, pellets were 3.97 in mm diameter, and experimental diets were fed for the first 14 d of the 28-d experiment. From d 0 to 7, pigs fed the SDAP diet conditioned at 60 degrees C had greater ADFI (P < 0.05) and tended (P = 0.12) to have greater ADG than pigs fed the diet without SDAP and conditioned at 60 degrees C. From d 0 to 7, ADG (quadratic effect, P < 0.03) and ADFI (linear effect, P < 0.002) decreased as conditioning temperature increased, with the largest decrease observed above 77 degrees C. From d 0 to 14 and 0 to 28, ADG was not affected (P > 0.10) by pellet conditioning temperature or SDAP fed from d 0 to 14. The results of these studies suggest that conditioning diets containing 5% SDAP at temperatures above 77 degrees C decreases weanling pig growth performance.     

Effect of dietary crude protein level on growth performance,blood characteristics,and indicators of intestinal health in weanling pigs

An experiment was conducted to test the hypothesis that reducing crude protein (CP) in starter diets for pigs reduces post-weaning diarrhea and improves intestinal health. In total, 180 weanling pigs were allotted to 3 diets containing 22, 19, or 16% CP. Fecal scores were visually assessed every other day. Blood samples were collected from 1 pig per pen on days 1, 6, 13, 20, and 27, and 1 pig per pen was euthanized on day 12. Results indicated that reducing dietary CP reduced (P < 0.01) overall average daily gain, gain to feed ratio, final body weight, and fecal scores of pigs. Pigs fed the 16% CP diet had reduced (P < 0.01) serum albumin compared with pigs fed other diets. Blood urea nitrogen, haptoglobin, interleukin-1β, and interleukin-6 concentrations in serum were greatest (P < 0.01) on day 13, whereas tumor necrosis factor-α and interleukin-10 concentrations were greatest (P < 0.01) on day 6. Villus height in the jejunum increased (P < 0.05) and crypt depth in the ileum was reduced (P < 0.01) if the 19% CP diet was fed to pigs compared with the 22% CP diet. A reduction (P < 0.05) in mRNA abundance of interferon-γ, chemokine ligand 10, occludin, trefoil factor-2, trefoil factor-3, and mucin 2 was observed when pigs were fed diets with 16% CP. In conclusion, reducing CP in diets for weanling pigs reduces fecal score and expression of genes associated with inflammation.     

Use of refined potato (Solanum tuberosum L. cv. Gogu valley) protein as an alternative to antibiotics in weanling pigs

This study investigates the use of refined potato protein (RPP) obtained from the potato (Solanum tuberosum) tubers of “Gogu valley” for their antimicrobial properties and its effects on growth performance, apparent nutrient digestibility and microbial populations in feces and large intestine of weanling pigs. Pigs (n = 280; Landrace × Yorkshire × Duroc; average initial body weight of 5.96 ± 0.66 kg; 23 ± 3 d of age) were allotted on the basis of their body weight and sex to five dietary treatments (each treatment comprised of 4 replicate pens with 14 pigs in each) in a randomized complete block design. The dietary treatments were: PC (positive control; basal diet + 150 ppm apramycin sulfate and 10 ppm colistin sulfate), and RPP (basal diet added with 0, 200, 400 and 600 ppm RPP). The RPP showed in vitro antimicrobial activity and at the concentration of 150 ppm inhibited the growth of tested microbes (Staphylococcus aureus, Salmonella choleraesuis, Salmonella gallinarum and Escherichia coli). Pigs fed with antibiotic diet showed better growth performance and had lower populations of total bacteria, coliforms and Staphylococcus spp. in the feces and large intestine when compared with pigs fed RPP diets. Increasing the levels of RPP in diets linearly improved performance and reduced the populations of total bacteria, coliforms and Staphylococcus spp. in feces and contents of colon and rectum. The apparent fecal digestibility of DM, CP and apparent ileal digestibility of amino acids did not differ among pigs fed antibiotic and RPP diets. These results suggest that antibiotics were more effective in improving the performance of pigs, while feeding of RPP also improved the performance and reduced the harmful microbes being more effective at higher levels. Thus at higher levels RPP obtained from the potato tubers of Gogu valley can be a potential replacement of antibiotics in the feed of weanling pigs.     

Use of double-choice feeding to quantify feed ingredient preferences in pigs

Four experiments were conducted to evaluate the palatability of sorghum, maize, rye and lupine for pigs. Diets containing sorghum, maize, rye or lupine (test diets) were offered in a series of double choice tests against a reference diet. To prepare the reference diets, white broken rice in the experiments with cereals, and SBM-56 (a soy protein product low in anti-nutritional factors) in the experiment with lupine, were used as the cereal and the protein source of reference, respectively. Six test diets containing sorghum, maize or rye were prepared by replacing either 250 or 500 g kg^− 1 of broken rice from the reference diet. Similarly, two test diets containing lupine were prepared by replacing 75 or 150 g kg^− 1 of SBM-56 from the reference diet. Additionally, the pure ingredients were also evaluated against pure broken rice (sorghum, maize or rye) and pure SBM-56 (lupine). In each experiment, the corresponding ingredient was tested at two levels of inclusion and in pure form, using both newly weaned pigs and pigs at four weeks post-weaning. The palatability for each test diet expressed as % of preference was calculated as the percentage contribution of the test diet to total feed intake (test + reference diets). The preferences obtained ranged between 16 and 29% for sorghum, 16 and 35% for maize and 19 and 49% for rye. Except for rye at 500 g kg^− 1 (49%), preference values were significantly different from 50% which indicates a higher preference for the broken rice in the reference diet than for sorghum, maize and rye. Preference for lupine ranged between 39 and 56% and no difference in preference was observed between the lupine in the test diets and the SBM-56 in the reference diets. The dietary preferences could already be observed in the first days of experiment, and they did not change substantially thereafter. The levels of inclusion tested did not have an effect on preference for any of the ingredients. The age of the animals did not affect preference and the values obtained in newly weaned and post-weaned pigs were generally in good agreement. However the use of the older animals resulted in higher feed intakes and more accurate measurements. It is concluded that feedstuff palatability can be quantified with a double choice protocol using a reference diet. This should allow the formulation of feeds for weaning pigs taking into account the palatability of the ingredients, in addition to other nutritional criteria. Preference evaluation may become a useful tool to improve the palatability of the diets and facilitate piglets feed initiation at weaning.     

Growth performance,carcass criteria,and serum biochemical parameters of broiler chickens supplemented with either synbiotic or prebiotic under hot climatic conditions

ABSTRACT

1. Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.

2. In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg^?1 of the starter diets and 0, 0.5 or 0.75 g kg^?1 of the grower diets) for 6 weeks. In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg^?1 of the starter diets and 0, 0.25 or 0.5 g kg^?1 of the grower diets) for 6 weeks. Each treatment had 10 replicates containing 5 broiler chickens each.

3. Supplementation of synbiotic at 1 or 1.5 g kg^?1 feed to broiler diets during 1–21 d and 0.5 or 0.75 g kg^?1 feed, respectively, during 22–42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.

4. Similarly, supplementation of the prebiotic at 0.5 or 1 g kg^?1 in broiler diets during 1–21 d and 0.25 or 0.50 g kg^?1 feed, respectively, during 22–42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.     

Corn protein has greater concentrations of digestible amino acids and energy than low-oil corn distillers dried grains with solubles when fed to pigs but does not affect the growth performance of weanling pigs

Three experiments were conducted to test the hypothesis that standardized ileal digestibility (SID) of amino acids (AA) and digestible energy (DE) and metabolizable energy (ME) in a new source of corn protein are greater than in corn distillers dried grains with solubles (DDGS) and that corn protein may be included in diets for weanling pigs. In experiment 1, the SID of AA was determined in two sources of DDGS (DDGS-1 and DDGS-2) and in corn protein. Results indicated that SID of most AA was greater (P < 0.05) in DDGS-2 and corn protein than in DDGS-1, but corn protein contained more digestible AA than both sources of DDGS. In experiment 2, the DE and ME in corn, the two sources of DDGS, and corn protein were determined. Results demonstrated that DE (dry matter basis) in corn protein was greater (P < 0.05) than in corn, but ME (dry matter basis) was not different between corn and corn protein. However, DE and ME in corn (dry matter basis) were greater (P < 0.05) than in DDGS-1 and DDGS-2. In experiment 3, 160 weanling pigs were allotted to four treatments in phases 1 and 2 and a common diet in phase 3. Corn protein was included at 5% to 10% in phases 1 and 2 at the expense of plasma protein and enzyme-treated soybean meal. Results indicated that although differences in average daily gain and gain to feed ratio were observed in phase 1, no differences among treatments were observed for the overall experimental period. In conclusion, the concentration of digestible AA is greater in corn protein than in DDGS; DE and ME in corn protein are also greater than in DDGS; and up to 10% corn protein may be included in phase 1 and phase 2 diets for weanling pigs.     

长白×北京黑F_1代猪适宜粗蛋白与赖氨酸、粗蛋白比的研究

用2个蛋白水平和2个赖蛋比值作2×2析因设计,将32头始重25公斤的长白×北黑F_1代猪随机分成4组,每组8头,进行饲养试验;将16头阉公猪也分为4组,每组4头,进行N—平衡试验,研究了以玉米—豆饼为基础的饲粮蛋白质和赖蛋比水平对猪在20—60公斤和60—90公斤两个体重阶段的生产性能,氮沉积和90公斤时胴体品质的影响。粗蛋白由16—14％降至14—12％时极显著降低了胴体瘦肉率、背最长肌蛋白含量和日氮沉积量,提高了背膘厚,对增重和饲料效率等无明显影响。赖蛋比4.5—4.5％提高到5.0—4.5％,除降低 日采食量、增重和屠宰率(P<0.05)外,对其余指标均无影响。本试验结果表明:长×北F_1代瘦肉猪饲粮适宜粗蛋白为16—14％,赖蛋比为4.5—4.5％。     

Effects of chitooligosaccharide supplementation on growth performance, nutrient digestibility, blood characteristics and immune responses after lipopolysaccharide challenge in weanling pigs

The objective of this study was to determine the effects of dietary supplementation with chitooligosaccharide (COS) on growth performance, nutrient digestibility, blood characteristics and immune response in lipopolysaccharide-challenged weanling pigs. A total of 90 crossbred weanling pigs (5.44 ± 0.50 kg BW) were employed in Exp. 1. The three dietary treatments were basal diets supplemented with 0, 2.5, and 5 g COS/kg, and fed for 28 d. Each treatment had 6 replications with 5 pigs per pen. Increasing the level of supplemental COS tended to linearly (P < 0.10) improve ADG and ADFI during phase 2 and overall period, while there were no differences in G:F. The linear improvement in the apparent DM (P < 0.05) and N (P < 0.10) digestibility in pigs fed COS supplemented diets was noticed. The tested blood characteristics were not influenced under non-challenge conditions. In Exp. 2, a total of 20 pigs (5.22 ± 0.31 kg BW) were initially assigned to two dietary treatments and fed basal diets supplemented with 0 or 0.5 g COS/kg for 28 d. At the end of d 28, half of the pigs in each treatment (n = 5) were injected i.p. with Escherichia coli lipopolysaccharide at a concentration of 100 μg/kg of BW. The other half of the pigs in each treatment were injected with sterile saline solution at a concentration of 100 μg/kg of BW. This arrangement resulted in a 2 × 2 factorial design with diet and LPS challenge as the main effects. Blood sample and rectal temperature data were collected at 0, 2, 4 and 12 h post-challenge. Rectal temperatures increased as the result of LPS injection at 4 and 12 h post-challenge (P < 0.05). Serum cortisol, IGF-1, and TNF-α concentration were also increased as the result of LPS challenge (P < 0.05). The COS treatments resulted in lower cortisol concentrations at 2 h and higher IGF-1 concentrations at 4 h post-challenge (P < 0.05). COS and LPS interactions were also observed on cortisol and IGF-1 when the COS effects were presented (P < 0.05). Haptoglobin concentrations remained unaffected throughout the challenge period. White blood cell counts were increased in the LPS-treated pigs at 2 and 4 h post-challenge (P < 0.01). Lymphocyte count was elevated at 2 h and reduced at 12 h post-challenge as the result of LPS challenge (P < 0.05). However, there were no COS main effects observed on lymphocyte count throughout the challenge period. The comparison between two LPS challenged treatments also indicated that COS treatment has beneficial effects on rectal temperature, cortisol and IGF-1 concentrations. In conclusion, dietary supplementation with COS had little effect on nutrient digestibility and inflammatory stress markers in weanling pigs.     

The effect of a <Emphasis Type="Italic">β</Emphasis>-mannanase-based enzyme on growth performance and humoral immune response of broiler chickens fed diets containing graded levels of whole dates

The use of low-quality whole dates in broilers’ diets was evaluated. The apparent metabolizable energy (AMEn) of whole dates and date pits was determined with or without a β-mannanase-based enzyme (Hemicell – a registered trademark of ChemGen Corp., Gaithersburg, MD. Dried Bacillus lentus fermentation solubles with 158 million units/kg minimum enzyme activity. Recommended usage rate is 0.4 g kg⁻¹ of feed. ChemGen Corp., Gaithersburg, MD, USA). A 3 × 2 factorial arrangement consisting of whole dates (0, 175, and 350 g kg⁻¹) and enzyme (0 or 0.4 g kg⁻¹) was performed to assess the effect of the enzyme on the nutritive value of whole dates (experiment 2) in broilers’ diet. Enzyme improved AMEn contents of the whole dates and date pits. The diets containing 175 or 350 g kg⁻¹ whole dates produced body weight gain (BWG) similar to those obtained without dates. Enzyme improved BWG and feed conversion ratio throughout the experimental period. In conclusion, whole dates can be included in broiler diets at levels up to 350 g kg⁻¹. In addition, β-mannanase supplementation would have beneficial effects on performance and immunity in birds fed on corn–soy or corn–soy–whole dates diets.     

Effects of replacing fish meal with catfish (Pangasius hypophthalmus) processing waste water on the performance of growing pigs

A feeding trial with growing pigs was carried out in which fish meal was replaced by different levels of Tra catfish (Pangasius hypophthalmus) by-product processing waste water (WW). A control diet included fish meal (FM) as the sole protein supplement (WW0), and there were four experimental diets in which 100% (WW100), 75% (WW75), 50% (WW50), and 25% (WW25), respectively, of the crude protein from FM in WW0 was replaced by WW. Thirty crossbred castrated (Yorkshire × Landrace) male pigs with an initial average body weight of 23.6 ± 1.6 kg were allocated into 30 individual pens in a randomized complete block design with six replications. Average daily feed intake and essential amino acid intakes were higher (P < 0.01) in WW0 and WW25 compared with the other three diets, and ether extract intake was highest in WW100 (P < 0.01). The highest average daily gain was in WW0 (582 g/day) and lowest in WW100 (501 g/day; P < 0.01). Dry matter feed conversion ratio was lowest in WW100 (2.16 kg feed/kg gain) and highest in WW0 (2.42 kg feed/kg gain) (P < 0.01).The cost/gain in pigs fed WW100 was lowest (12,476 VND/kg gain), and was highest in WW0 (18,312 VND/kg gain). In conclusion, although performance is reduced, it is possible to replace up to 100% of the fish meal by catfish by-product processing waste water in diets for growing pigs, resulting in much lower feed costs.