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1.
Protein bodies ontogeny and localization of prolamin components in the developing endosperm of wheat caryopses 总被引:1,自引:0,他引:1
During caryopsis development, prolamins are initially stored in individual protein bodies, then generate a protein matrix in the ripe caryopsis. The ontogeny of the protein bodies was analyzed by fluorescence and electron microscopy from 7 to 43 days after anthesis (dAA), a period of time from the cellularization of endosperm to its desiccation. A series of antibodies specific to each prolamin type (α/β-, γ-, ω-gliadins, low-molecular weight and high-molecular weight glutenin subunits) made it possible to localize and co-localize the different prolamins in organelles of endosperm cells at different developmental stages. Protein bodies containing prolamins were observed as early as 7 dAA. At the early developmental stages, protein bodies were spherical with diameters around 1–2 μm. Later, around 15 dAA, the PBs enlarged, and aggregation and/or coalescence were prominent at 21 dAA. From 33 dAA, individual PBs were no longer visible, but a protein matrix was confined in the space between starch granules. All prolamins were found in the same protein bodies, without any segregation according to their types. Immunochemical labelling of prolamins failed to reveal in TEM analyses any particular internal organization in protein bodies. Glutenin subunits and gliadins were observed in the Golgi apparatus at the early stages of endosperm development. 相似文献
2.
The relative quantity of specific proteins, protein subunits, as well as amount and size-distribution of polymeric proteins in wheat kernels may vary due to environmental conditions. In this study, the effect of different nitrogen treatments on polymeric and monomeric proteins in wheat was determined. Two soft white biscuit wheat cultivars, a cracker wheat and a hard red bread wheat were planted under irrigation in a randomized complete block design with three replications in two successive years at six different nitrogen treatments. SE-HPLC was used to determine the amount of monomeric and polymeric proteins, and various quality characteristics were measured after harvesting. The large and small SDS-extractable polymeric proteins were not influenced by different N levels. There was a strong cultivar influence, where some cultivars had a larger reaction to N treatments than others. The later application (at flag leaf stage) of nitrogen did not increase the protein fractions. The total amount of N given seemed to have a greater influence on the protein fractions than the timing of the fertilizer application. The lowest N treatment consistently gave the lowest flour protein content value. There was a strong correlation between flour protein content and large monomeric proteins. 相似文献