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20%地菌灵不同浓度处理对苦瓜嫁接影响 总被引:1,自引:0,他引:1
在相同基质条件下,研究20%地菌灵的不同浓度对苦瓜嫁接砧木"银光"根系抗病性的影响。结果表明:处理3(20%地菌灵1 000倍液消毒)对苦瓜嫁接砧木"银光"感染基质病菌的防治效果最为显著。 相似文献
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嫁接对苦瓜在水渍条件下的产量及某些生理特性的影响 总被引:7,自引:0,他引:7
以有棱丝瓜、无棱丝瓜、黑籽南瓜和瓠瓜作砧木,苦瓜作接穗进行嫁接栽培试验.有棱丝瓜作砧木插接法的嫁接成活率最高,达92.66%.有棱丝瓜和黑籽南瓜作砧木的苦瓜嫁接苗比自根苗提早进入开花结果期.水渍条件下两种砧木嫁接植株比自根苗植株分别增产36.5%和 33.7%,同时以有棱丝瓜作砧木的嫁接植株的叶绿素、氮、磷、钾含量和伤流量以及植株体内保护性酶(SOD、POD、CAT)的活性均大于自根苗植株和以黑籽南瓜作砧木的嫁接植株,而膜脂过氧化产物(MDA)的含量最低. 相似文献
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分别以黑籽丝瓜、黑籽南瓜、白籽丝瓜为砧木,与兴合苦瓜进行嫁接,研究比较了3种砧木对苦瓜嫁接亲和力、生长发育、果实商品性、产量及抗逆性的影响。试验结果表明,3种砧木与苦瓜接穗亲和力较强,均能达到95%及以上,其中以白籽丝瓜嫁接后,苦瓜生长势强、抗病及抗旱性好、商品性状最优、产量最高。综合比较,白籽丝瓜是苦瓜嫁接的理想砧木,适合在生产中推广应用。 相似文献
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以‘特选一号’甜瓜为接穗,‘银光’南瓜为砧木,采用了顶插接、改良插接、断根插接和断根贴接4种嫁接方法,研究了不同嫁接方法对甜瓜嫁接苗生长的影响,以期为甜瓜育苗提供指导。结果表明:甜瓜顶插接嫁接速率最快、嫁接工效最高,嫁接后在昼/夜温度为28℃/18℃条件下,改良插接的嫁接成活率最高,改良插接和断根贴接的愈合时间短。在一叶一心时期,改良插接和顶插接的嫁接苗有明显的生长优势,改良插接的嫁接苗根系生长最好;而到三叶一心时期,断根插接和断根贴接的甜瓜嫁接苗生长快,壮苗指数显著高于顶插接和改良插接,断根插接的嫁接苗叶面积最大。不同嫁接方法所需除萌蘖次数和时间也存在差异,断根插接除萌蘖次数最少,除萌蘖用时仅为顶插接的46.3%。 相似文献
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为研究核桃内果皮硬化主要涉及的生物过程及生长素在硬化过程中的作用,以‘新露’核桃为研究试材,形态学观察及木质素沉积分析表明,内果皮硬化与其表面维管束分布具有密切关系,靠近维管组织附近的内果皮硬化先于远离维管的区域。IAA含量在内果皮硬化过程中呈V字形变化;IAA在5个时期平均含量最高(16.467 ng·g-1),约为IBA、ICA和ME-IAA总和的6.8倍。木质素合成中间代谢物分析显示,木质素单体对香豆醇在内果皮硬化区的含量是未硬化区的16倍,芥子醇在内果皮硬化区的含量极高,但在未硬化区未检测到存在,且随着硬化进程这两种物质在内果皮硬化区的含量总体呈上升趋势。转录组与蛋白组关联分析获得了内果皮硬化相关的差异表达基因369条,在转录水平和蛋白质水平同时存在差异的基因关联结果较好,相关系数最低为0.48124;但差异表达基因整体关联结果较差,相关系数最高为0.22112。利用Metascape构建了差异表达基因显著富集的前20个生物学功能之间的关系网络,发现内果皮硬化主要涉及苯丙烷类代谢、氧胁迫响应、细胞壁组织与生物合成和氨基糖与核酸糖代谢4个生物过程。荧光定量结果表明,AUX/IAA... 相似文献
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AIM and METHOD:To determine the production of nitric oxide(NO) and change of NO synthase(NOS) activity in mitochondria isolated from the rat brains of the ischemia/reperfusion rat model produced by transient occlusion of middle cerebral artery on the following time points:2 h after occlusion of artery and 30 min,2 h, 4h after reperfusion.RESULTS:After the occlusion of middle cerebral artery,the respiratory control rate(RCR) of mitochondria significantly decreased and slightly increased at 4h after reperfusion.Meantime,the production of NO in mitochondria increased significantly.But with the increase of perfusion, production of NO gradually decreased and reached normal level as in the control group.It also shows that cerebral ischemia increased NOS's activity significantly in the mitochondria and still kept a higher level than the control group although it decreased gradually after reperfusion.But the iNOS's activity did not show obvious change.The change of total NOS's activity depends on the change of cNOS's activity.CONCLUSION: The activation of NO/NOS system in the mitochondria might play an important role in the reperfusion injury during reperfusion of ischemic brain. 相似文献
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AIM:To evaluate the changes of lung morphologic features and oxidative stress in experimental diabetic rats. METHODS: The pulmonary structure of alloxan-induced diabetic rats were quantitatively studied with stereological methods. Changes of ultrastructure, activities of superoxide dismutase (SOD) and contents of malondialdehyde(MDA) of diabetic lung and serum were observed. RESULTS: The volume proportion of alveolar air and mean linear intercept of diabetic rats decreased remarkably in comparison with controls while the volume proportion of alveolar wall, the surface density of alveolar, the numerical density of alveolic area, the numerical density of alveolar and specific surface of alveolar increased significantly. The major change of the type II pneumocyte of diabetic rats was dilation of the rough endoplasmic reticulum (RER). The other findings in diabetic rats had included the presence of thickened alveolar epithelial, pulmonary capillary basal laminae and blood- air barrier, the volume density, the surface density, the mean profile area and the mean perimeter of RER in type Ⅱ pneumocyte of diabetic rats increased remarkably. And the specific surface of RER was significantly lower as compared with controls. SOD activity decreased and MDA content increased significantly in serum of diabetic rats as compared with the control group. SOD activity in the diabetic lung was not different from that of the control lung. However, the content of MDA obviously increased in diabetic lung. CONCLUSION: The morphologic features and oxidative stress in early diabetic rats are abnormal thus the lung should be considered as one of the "target organ"in diabetes mellitus. 相似文献
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苗期机械拂擦处理对蔬菜植株生长和发育的影响 总被引:1,自引:0,他引:1
探明育苗期应用机械拂擦处理代替植物生长延缓剂矮化植株、控制幼苗徒长的可能性。机械拂擦处理强度通过接受处理时间迟早及塑料刷片密度高低来调节。结果表明机械拂擦处理显著延缓植株生长 ,缩短植株节间长度 ,提高茎粗和株高比。甜椒、番茄、黄瓜对机械拂擦处理的敏感性不一 ,生长延缓程度随着处理强度的增加而增加。机械拂擦处理对始花期有一定的影响 ,但对果实产量无不良影响 相似文献
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XU Li LV Li-hong YU Jian-dong LONG Tian-zhu HUANG Yong-heng MIN Jun WAN Yun-le 《园艺学报》2010,26(11):2091-2096
AIM: To investigate the role of allicin on the anti-tumor activity of rat NK cells in vitro. METHODS: CD3-NKR-P1+ NK cells were isolated from rat spleen using Dynabeads FlowCompTM Flexi system. The proliferation and apoptosis of the NK cells induced by allicin were analyzed by the method of flow cytometry. The levels of IFN-γ in the supernatants were measured by ELISA. Lactate dehydrogenase release assay was used to detect the cytotoxicity of the NK cells.RESULTS: In vitro, allicin significantly promoted the proliferation of NK cells, inhibited NK cell apoptosis, increased the level of IFN-Fγ in supernatants and enhanced the cytotoxicity of the NK cells to Yac-1 tumor cells in a dose-dependent manner. Allicin at concentration of 30 mg/L exerted the maximal effect on activating NK cells. CONCLUSION: Allicin enhances the activity of NK cells in vitro by upregulating IFN-γ expression. 相似文献
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采用5种不同光质光源,分别对真姬菇(Hypsizygusmarmoreus)的菌丝、原基和子实体进行照射,检测菌丝生长速度、原基形成数量、子实体菌柄长度、菌柄直径、菌盖直径以及产量等。结果显示:真姬菇菌丝在黑暗和红光下生长较快,在红绿蓝复合光下生长最慢;在蓝光下形成的原基数量最多,而在红光下原基数量最少;子实体阶段,黑暗下菌柄的长度及直径最大,绿光下菌柄长度及直径最小;而在蓝光和红绿蓝复合光下菌盖直径最大。相同培养时间,真姬菇在黑暗和蓝光下产量较高,在绿光下产量最低。进一步检测真姬菇光受体基因WC1和WC2在不同光质条件下的差异表达。结果表明,在菌柄中WC1在黑暗下表达量最高,WC2在蓝光下表达量最高;而在菌盖中WC1和WC2均在蓝光下表达量最高。 相似文献