海口秀英港区船舶进出港通航方案比选

航道通航方案的选择直接影响到航运效益和通航安全,本文运用模糊层次分析法对海口秀英港区船舶进出口航道通航方案进行了比选,经过评价比选得出,经上海海事大学研究的部分航段进行有条件的双向通航方案,在通航安全,通航经济效益和海事监管难度等综合方面较为合理。     

二辛可宁酸试剂定量蛋白质与口蹄疫抗原的研究

二辛可宁酸(bicinchonininc acid,BCA)可用于精确定量蛋白质。用不连续蔗糖密度梯度离心的方法纯化口蹄疫146S抗原。BCA试剂定量0～200 μg/ml的白蛋白和该纯化抗原,紫外分光光度计560 nm波长读取结果（OD值）,白蛋白检测结果用SPSS软件进行线性和回归分析,发现数据相关性、线性显著,计算出了回归方程,绘制出了蛋白质含量与OD值之间的标准曲线。将纯化抗原的数据代入回归方程计算出该抗原的含量。     

面向智能航道 创新测绘技术

为了使航道测量更好的应用到数字航道建设中,本文采用创新测绘技术,利用现代测绘技术及工程实例对内河航道测绘的方法进行了探究,得到了GPSRTK+测深仪+成图软件的现代内河航道测绘方法。该方法不仅具有便于操作、精度高、速度快的特点,还能全天候工作,为长江电子航道图提供实时数据。这种方法不仅为数字航道的建设提供了坚实基础,也为今后的航道测绘研究提供了参考。     

学生网上评教数据的优化处理研究

学生网上评教已经成为许多学校教学质量监控的主要手段之一,学生评教数据的准确性直接影响到评教结果的应用,对于学校教学质量监控至关重要。通过研究判断和筛选失真的评教数据的方法,提高学生网评结果真实度的途径,优化网评软件,对网评数据进行有效处理等问题,以使网评结果贴近教师授课的真实情况,为教学质量监控提供真实有效的数据。     

基于GPMS平台的配网运行分析系统

目前，国内配网运行工作缺乏有效管理，数据来源的多样化、数据总量的庞大及分散都严重制约着电网的发展。建立基于GPMS平台的配网运行系统，能够有效提高配网的运行水平，实现对庞大运行数据的集中查询、分析与运用。本文首先简单介绍了我省的电网生产管理系统，然后通过系统建设目标、架构设计以及功能设计等方面的分析，阐述了GPMS系统对配网运行分析的重要辅助作用。     

“十二五”期间江苏省航道科技研究现状及发展规划

随着江苏省经济的腾飞以及党中央及相关部门对水路运输的进一步重视,"十二五"期间对江苏省航道科技的建设有了进一步的提高要求,在此背景下,本文根据江苏省交通部、江苏省航道局以及江苏省统计局等单位所统计的数据、资料,对江苏省航道科技现状进行了分析。在此基础上,结合党中央及相关部门对江苏省航道科技的发展需求,探讨了江苏省航道科技的发展规划,为江苏省航道科技进一步发展建设提供了理论依据与发展方向。     

直驱永磁同步风力发电机组建模与仿真

本文针对兆瓦级的永磁直驱同步风电机组(D-PMSG)多运行于并网发电状态,在DIg SILENT/Power Factory仿真软件中搭建了基于双PWM全功率变流器的D-PMSG并网仿真模型;阐述了系统的运行原理,对机侧和网侧的变流器的控制策略进行了详细的分析,该系统能够实现风能最大功率追踪以及并网控制,仿真结果验证了所建模型的正确性和控制策略的可行性。     

利用Excel函数自动批量判定口蹄疫ELISA试验数据的方法

<正>目前,国内的大部分兽医实验室都具备利用ELISA试验检测口蹄疫免疫抗体的技术能力。酶标仪对酶标反应板进行读数后,给出的是96个原始的OD值,需要手动计算才能分析出近百个精确到小数点后4位的数,得出最终结果,费时费力,就锦州地区来说,2014—2015年就有4万多份样品进行了ELISA试验检测,2 000多张OD值原始数据单需要手动分析,需要20万次人工运算和手写。这样对原始数据     

GPS技术在高精度工业建设厂区控制网中的应用

以某工业建设厂区高精度测量控制网的建设为例,介绍如何使用GPS测量技术在工业建设厂区建立测量控制网,并分析布点和外业观测中所遵循的要求,简述了GPS数据处理的一般过程及精度分析。     

检测鸭坦布苏病毒卵黄抗体间接ELISA方法的建立

本研究以纯化的鸭坦布苏病毒WR株为包被抗原,建立检测鸭坦布苏病毒卵黄抗体的间接ELISA方法。经优化后确定检测样品的结果P/N([样品孔OD450nm-空白孔OD450nm)/(阴性孔OD450nm-空白孔OD450nm)]≥2.1判为阳性,<1.5者为阴性;1.5≤P/N<2.1者判为可疑,重检后仍可疑者定为阴性。以建立的间接ELISA方法对H9亚型禽流感病毒、H5亚型禽流感病毒、减蛋综合征病毒、鸭瘟病毒和大肠杆菌卵黄抗体进行了检测,结果均为阴性,表明该方法具有良好的特异性。经重复性试验表明,该方法重复性好。本方法的建立对开产种鸭和蛋鸭坦布苏病毒疫苗免疫效果的评价具有十分重要的意义。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.