Effects of leaf scales of different pineapple cultivars on the epiphytic stage of Fusarium guttiforme

The fungus Fusarium guttiforme (Syn. F. subglutinans f. sp. ananas) is responsible for fusariosis, one of the main phytosanitary threats to pineapple (Ananas comosus var. comosus). A structural study comparing epidermal differences in pineapple cultivars resistant and susceptible to fusariosis was performed, relating properties of the epidermis to known susceptibility to the disease. The basal, non-chlorophylled, portions of mature leaves of pineapple plants were analyzed by light and electron microscopy. All cultivars showed common morpho-anatomic aspects characteristic of Bromeliaceae, such as scutiform scales and unstratified epidermis. However, cultivar Vitoria (resistant) had less scales than cultivars Smooth Cayenne (susceptible, intermediate severity) and Perola (susceptible, with extreme severity of fusariosis symptoms). Inoculation of conidia suspension (10⁵ conidia ml⁻¹) of the fungus F. guttiforme to leaves and harvesting 24 h later yielded numbers of viable colonies related to the density of leaf scales. This suggests that scales can act as havens for fungal conidia and favour the epiphytic stage of the fungus on pineapple plants, and are involved in the interaction of plant and pathogen. A reduction in scale numbers was related to lower infection levels and is relevant to the future breeding programme for development of new pineapple cultivars resistant to fusariosis and their involvement in integrated control strategies.     

The effects of nitrogen rate and the ratio of NO3:NH4 on Bemisia tabaci populations in hydroponic tomato crops

Nitrogen fertilization is one of the factors that influences Bemisia tabaci (Gennadius) population density. The aim of this study was to determine the effects of three N application rates (75, 205 and 335 mg/l) and three ratios of NO₃⁻:NH₄⁺ ions (92:8, 75:25 and 55:45) in standard nutrient solution (205 mg/l N) on the population density of B. tabaci. The experiments were conducted on spring-summer hydroponic crops of tomato. The effect of plant stratum on the whitefly population was also determined. The aggregation of B. tabaci adults as well as their oviposition rate was higher at 205 and 335 mg/l N than on plants grown at 75 mg/l N. By the end of the experiment (60 d after infestation), the number of nymphs on plants at 205 mg/l N was higher than on plants at 75 mg/l N. The number of pupae was lowest on plants supplied with 75 mg/l N. An increase in NH₄⁺ percentage in standard nutrient solution (from 25% to 45% of the total N) reduced adult population density and oviposition rate. The density of nymphs and pupae, at 60 d after infestation, was lower on the tomato plants grown at 75:25 and 55:45 NO₃⁻:NH₄⁺ ratios compared to the 92:8 ratio. The 75:25 and 55:45 NO₃⁻:NH₄⁺ ratios resulted in a higher incidence of blossom-end rot of tomato fruit, with a lower incidence of disorder at 75:25 than at the 55:45 ratio. Plant stratum influenced adult whitefly distribution in two years of the study. Middle stratum leaves were more attractive to adults in both years. The results demonstrate the effects of N fertilization (N rate and the ratio of NO₃⁻:NH₄⁺) and plant stratum on B. tabaci population density.     

High level of resistance to Sclerotinia sclerotiorum in introgression lines derived from hybridization between wild crucifers and the crop Brassica species B. napus and B. juncea

Sclerotinia rot caused by the fungus Sclerotinia sclerotiorum is one of the most serious and damaging diseases of oilseed rape and there is keen worldwide interest to identify Brassica genotypes with resistance to this pathogen. Complete resistance against this pathogen has not been reported in the field, with only partial resistance being observed in some Brassica genotypes. Introgression lines were developed following hybridization of three wild crucifers (viz. Erucastrum cardaminoides, Diplotaxis tenuisiliqua and E. abyssinicum) with B. napus or B. juncea. Their resistance responses were characterized by using a stem inoculation test. Seed of 54 lines of B. napus and B. juncea obtained from Australia, India and China through an Australian Centre for International Agricultural Research (ACIAR) collaboration programme were used as susceptible check comparisons. Introgression lines derived from E. cardaminoides, D. tenuisiliqua and E. abyssinicum had much higher levels (P < 0.001) of resistance compared with the ACIAR germplasm. Median values of stem lesion length of introgression lines derived from the wild species were 1.2, 1.7 and 2.0 cm, respectively, as compared with the ACIAR germplasm where the median value for stem lesion length was 8.7 cm. This is the first report of high levels of resistance against S. sclerotiorum in introgression lines derived from E. cardaminoides, D. tenuisiliqua and E. abyssinicum. The novel sources of resistance identified in this study are a highly valuable resource that can be used in oilseed Brassica breeding programmes to enhance resistance in future B. napus and B. juncea cultivars against Sclerotinia stem rot.     

Caesalpinia spinosa and Castanea sativa tannins: A new source of biopolymers with adsorbent capacity. Preliminary assessment on cationic dye removal

Tannins from Caesalpinia spinosa (Tara) and Castanea sativa (Chestnut) have been tested for gelification. Adsorbent are produced by formaldehyde-induced polymerization (TTG for Tara, CTG for Chestnut). These new biopolymers were tested for cationic dye removal and promising results were achieved: gelified tannins from both natural vegetal sources were effective in removing Victoria Blue R, Methylene Blue (MB) and Fuchsine, while Auramine O and Rhodamine B were refractory to this treatment. The particular case of CTG-MB is ruled by Langmuir adsorption hypothesis. k_l1 and k_l2 parameters are obtained for four different temperatures according to linear, non linear and multiparametric adjustment. Good r² correlation coefficients are obtained in every case.     

Wilt disease management and enhancement of growth and yield of Cajanus cajan (L) var. Manak by bacterial combinations amended with chemical fertilizer

Root nodulating Sinorhizobium fredii KCC5 and Pseudomonas fluorescens LPK2 were isolated from nodules of Cajanus cajan and disease suppressive soil of tomato rhizosphere, respectively. Both strains produced IAA, siderophore, solubilized insoluble phosphate, showed chitinase and β-1,3-glucanase activities, and strongly inhibited the growth of Fusarium udum. It also caused degradation and digestion of cell wall components, resulting in hyphal perforations, empty cell (halo) formation, shrinking and lysis of fungal mycelia along with significant degeneration of conidia. LPK2 produced volatile cyanogen (HCN). Combinations of S. fredii KCC5 and P. fluorescens LPK2 with half dose of chemical fertilizers showed a significant increase in seed germination (94%) while seed germination with co-inoculated strains (KCC5 + LPK2), KCC5 and LPK2 alone was 90, 84 and 82% respectively as compared to control 77%. After 120 days of sowing, per plant number of pods, nodules, shoot length, root length, shoot weight and root weight were greater for the combination with half dose of chemical fertilizers compared to the control. Combinations of S. fredii KCC5 and P. fluorescens LPK2 with half dose of chemical fertilizers resulted in an 82% increase in grain yield per hectare compared to the control. Both strains KCC5 and LPK2 led to proto-cooperation as evidenced by synergism, aggressive colonization of the roots, and enhanced growth, suggesting potential biocontrol efficacy against Fusarium wilt in C. cajan.     

Screening of three sweet potato (Ipomoea batatas L.) cultivars for resistance to different virulence groups of root-knot nematodes (Meloidogyne spp.) under controlled conditions

The susceptibility of three sweet potato cultivars (Ipomoea batatas L.) C4, TIS 3290 and TIS 9162 was evaluated against 156 isolates of Meloidogyne spp. with the aim to include resistant/tolerant sweet potato cultivars in a crop rotation scheme for the management of root-knot nematodes. The nematode isolates corresponded to races 1, 2 and 3 of Meloidogyne arenaria (n = 7), races 1, 2, 3 and 4 of M. incognita (n = 131) and Meloidogyne javanica (n = 18). Also, the isolates of M. incognita were differentiated in virulence groups: Pepper (n = 35), Pepper-Mi (n = 25), Tomato (n = 41) and Tomato-Mi (n = 30), depending on their ability to parasitize resistant pepper and tomato cultivars. The tested isolates of M. javanica parasitized C4 and TIS 3290, but not TIS 9162, whereas M. arenaria parasitized C4 and TIS 9162, but not TIS 3290, and M. incognita was able to parasitize the three sweet potato cultivars tested. C4 was the most susceptible cultivar to all nematode species tested, especially M. incognita, TIS 3290 was the most resistant and TIS 9162 was in between (7.2, 62.9 and 26.9% of resistant plants, respectively). Susceptibility of the sweet potato cultivars showed slight variations depending on the race or virulence group of M. incognita. The results suggest that sweet potato cultivars TIS 3290 and TIS 9162 may be used as rotation crops in fields where root-knot nematodes are present, their selection depending on the Meloidogyne isolates present. The use of resistant sweet potato cultivars would be preferably combined with other management practices to avoid virulence selection in nematode isolates.     

Effectiveness of plant extracts on suppression of damping-off and wilt diseases of lupine (Lupinus termis Forsik)

The aim of this study was to evaluate water and organic solvent of plant extracts for protection of lupine plants against damping-off and wilt diseases caused by Fusarium oxysporum f. sp. lupini, F. oxysporum f. sp. lupini Snyder & Hansen was isolated from diseased lupine roots collected from different locations of Minia, Assiut and New Valley governorates. Water leaf extracts of Calotropis procera, Nerium oleander, Eugenia jambolana, Citrullus colocynthis, Ambrosia maritime, Acacia nilotica and Ocimum basilicum and fruit extracts of C. colocynthis, C. procera and E. jambolana reduced damping-off and wilt diseases caused by F. oxysporum f. sp. lupini. Water extracts of E. jambolana leaves, C. colocynthis fruits and N. oleander leaves were the most effective ones for controlling such diseases. In contrast, A. nilotica and O. basilicum extracts were the least effective ones. Organic solvent extracts of tested plants reduced damping-off and wilt diseases than water extracts. Butanolic and ethereal extracts were highly effective in reduction of diseases than the other tested extracts. Under field conditions, during winter growing seasons 2008/09 and 2009/10, ethereal and butanolic extracts of N. oleander and E. jambolana leaves and C. colocynthis fruits significantly reduced the percentage of wilt severity as well as improved growth parameters (plant height, number of branches, pods and seeds per plant) and increased seed index, total seed yield/hectare compared with control treatment, while protein content in seeds was not effected. In conclusion, our study demonstrated that some plant extracts can be used for biocontrol of lupine damping-off and wilt diseases.     

Influence of planting date on growth, artemisinin yield, seed and oil yield of Artemisia annua L. under temperate climatic conditions

Artemisia annua L. is an annual aromatic antibacterial herb, with effective antimalarial properties due to the presence of artemisinin. The intention of the present study was to establish plant survival, growth attributes, yield attributes and artemisinin yield of A. annua cv CIM - Arogya with different transplanting months in two cropping seasons (March 2005-February 2006 and March 2006-February 2007) under temperate climatic conditions of Himalaya, India. Artemisinin yield in the dried leaves was found maximum amongst the plants that were transplanted in March (24.39 kg ha⁻¹) and minimum in those transplanted in November (3.39 kg ha⁻¹).     

Influence of plant leaf surface on the development,reproduction and life table parameters of the predacious mite, Agistemus exsertus Gonzalez (Acari: Stigmaeidae)

The influence of leaf surfaces of different host plants on the biology and life table parameters of the predacious mite, Agistemus exsertus Gonzalez was studied in the laboratory. A. exsertus successfully developed and reproduced on all tested plant leaves. Smooth, glabrous pear leaves accelerated the development of A. exsertus and showed the shortest mean generation time. Slightly rough, slightly hairy leaves of mulberry and acalypha were the most suitable surface for oviposition of the predator. These leaves exhibited the highest fecundity and net reproductive rate. On the other hand, leaves of rough, pubescent apple and scabrous, bristly lantana were the least suitable surface for oviposition of A. exsertus. They showed the lowest fecundity and net reproductive rate. Acalypha leaves exhibited the highest intrinsic rate of increase for the predator, whereas lantana leaves showed the lowest value. This difference in the predator response to leaf surfaces of host plants should be considered to enhance the role of the predator in biological control programs.     

Variation in essential oil and fatty acid composition in different organs of cultivated and growing wild Ruta chalepensis L.

The essential oil and fatty acid composition of two provenances of Ruta chalepensis from four organs (leaves, flowers, stems and fruits) was determined. The effect of the plant part on total fatty acid contents, essential oil yields, fatty acid and volatile constituents was significant.Fatty acid profiles varied significantly among the studied provenances and organs. Linolenic acid had the highest amount in leaves of the two provenances. From R. chalepensis, in all organs, the main fatty acids were palmitic (13.10-25.31%), followed by palmitoleic (0-15.72%), stearic (1.03-6.85%), oleic (1.90-24.04%), arachidic (0.11-4.03%), eicosatetraenoic (0.10-5.60%) and behenic (0.47-6.09%) acids. Saturated fatty acids had the highest amounts in growing wild R. chalepensis flowers, and cultivated R. chalepensis stems were characterized by the predominance of polyunsaturated fatty acids. Oil composition of all studied organs has a healthy and nutritionally value. Essential oil yields varied from 0.39% to 2.46% and showed a remarkable variation with plant organs. Thirty-six volatile compounds were identified in different analyzed essential oils; 2-undecanone, 2-nonanol and 2-dodecanone had the highest percentages.