Effects of Rhizobium inoculation,organic and chemical fertilizers on yield and physical properties of faba bean seeds

A field experiment was carried out to investigate the effect of Rhizobium inoculation, sulphur, nitrogen and chicken manure on yield, 100-seed weight, cookability, non-soakers, total defects and hydration coefficient of faba bean. The results showed that sulphur, nitrogen and chicken manure treatments significantly (p 0.05) increased yield, 100-seed weight, non-soakers, and hydration coefficient, in the absence of Rhizobium inoculation. The results also showed that Rhizobium inoculation significantly (p 0.05) increased yield, 100-seed weight, cookability, but decreased non-soakers. A positive correlation (r=0.90) was observed between the non-soaker percent and the total defect percent. No correlation was found between non-soakers, hydration coefficient and cookability. The results of this investigation indicate that Rhizobium inoculation is a promising fertilizer because it is cheap, easy to handle and improves plant growth and seed quality. The efficiency of inoculation could be improved with the addition of biological, chemical or organic fertilizers. Generally, fertilization of faba bean with nitrogen, sulphur or chicken manure not only increased plant growth and yield, but also improved seed quality and nutritional value.     

Blanching of green bean (Phaseolus vulgaris)

Experiments with one and two steps blanching of green beans have been carried out. Inactivation of the peroxydase requires more heating than inactivation of the enzymes which gives rise to off flavour from aldehydes. When blanching for about one minute to inactivate lipoxygenase, aldehyde formation of flavour ceases. The content of vitamin C decreases during blanching according to a first order reaction. Since considerable loss of vitamin C occurs during blanching, the treatment time should be reduced to a minimum. During preblanching at 65–75 °C and final blanching, chlorophyll is degraded to pheophytin and the surface colour expressed by the Hunter-values (–a/b) increases with time which means that the colour of the beans changes from green to yellow. The firmness of beans, which was measured by use of a tenderometer, decreases during blanching according to a first order reaction with 40 kcal/mole activation energy. Preblanching at 65–75 °C increases the firmness of the beans linearly with treatment time. This increase in firmness is stable after final blanching at 95 °C and even after thawing of frozen beans.     

Comparison of procedures to determine protein content of developing bean seeds (Phaseolus vulgaris)

Micro-Kjeldahl, Lowry and Bradford procedures were compared for determining the protein content ofPhaseolus vulgaris seeds during their development. Micro-Kjeldahl and biuret techniques were also compared with mature seeds of a normal and a genetically-improved bean cultivar. The protein contents of casein and soy protein isolate were as well estimated by these four methods. For many samples of both bean and food protein products large disagreements were found between micro-Kjeldahl and the other three procedures.     

Effect of sowing date on the growth and seed yield of common bean (Phaseolus vulgaris L.) in highland environments

Evaluations of common bean cultivars in the highlands of Mexico indicated that land races from that region experience less reduction in seed yield and seed size in late sowings than do lines from other regions. Introduced materials are of interest as sources of increased disease and pest resistance and tolerance to edaphic constraints, however. To quantify effects of sowing date and determine possible underlying causes, germplasm of diverse origins was evaluated at two sites in the highlands using multiple sowing dates. In all trials, seed yield, seed weight, harvest index and canopy dry weight decreased with late sowings. Large effects of sowing date, cultivar and their interaction were found for the four traits. The possible importance of phenology per se and of weather conditions was first examined using regression analyses. Variation in seed yield, seed weight, harvest index and canopy dry weight was more closely associated with time to maturity than with time to flowering. Of three weather variables examined, minimum temperature during seed filling revealed the strongest relations with the four traits. Few interactions of any parameter with line were significant, indicating that the cultivars did not have a strong differential response to a specific weather condition such as night temperature. Simulation analyses comparing photoperiod-sensitive and day-neutral cultivars indicated that radiation and temperature explained part of the yield reduction with late sowings. Daylength also had an influence, however, even in the day-neutral cultivar. Given that phenology had a strong effect on yield and that its inheritance is better understood than that of other physiological traits, priority should be given to understanding the genetic basis of the response of cultivar phenology to sowing date in the region.     

Effect of alkali treatments on the nutritive value of common bean (Phaseolus vulgaris)

The effect of alkali treatments of common bean seeds with red seed coat on the stability of antinutritional factors such as tannins, phytates and trypsim inhibitors, vitamins such as niacin and riboflavin and on protein quality has been studied. The samples were processed by soaking and soaking and pressure cooking in alkalies such as sodium hydroxide, sodium carbonate and sodium bicarbonate. At low temperature sodium carbonate and at high temperature sodium bicarbonate were found to be most effective in the extraction of tannins. At both low and high temperatures sodium carbonate was found to be more efficient in destruction of phytates. In the case of trypsin inhibitors, extraction at both low and high temperatures with sodium bicarbonate was most effective. Sodium hydroxide treatment was found to be better as far as the retention of niacin and riboflavin was considered.     

Limitations of corn (Zea mays) and common bean (Phaseolus vulgaris) diets as protein and calorie sources

Mixtures of 70:30 and 87:13 of corn and beans with or without vitamins, minerals, limiting amino acids and calories were fed to 5-week-old pigs to determine performance and efficiency of utilization. After 12 weeks it was shown that increasing the ratio from 87:13 to 70:30 (corn:beans) was a desirable measure as was the simultaneous supplementation with the other nutrients; individual groups of nutrients resulted in partial improvement in animal performance. None of the diets used was capable of inducing growth similar to the control groups fed a corn:soya diet. Results confirm the importance of total nutrients to obtain increased efficiency of protein utilization; furthermore, diets, such as the corn:bean diets studied, need to be improved in relation to both quality and quantity of protein.INCAP Publication I-1144     

Comparative study of enzymes related to proline metabolism in tepary bean (Phaseolus acutifolius) and common bean (Phaseolus vulgaris) under drought and irrigated conditions,and various urea concentrations

There are several mechanisms used by plants for survival in adverse environments such as drought, high temperature and salinity. The objective of this study was to evaluate the drought tolerance of tepary bean as a function of biochemical processes linked to isozyme synthesis and changes in enzymatic activity related to proline metabolism. Mature seeds of common beans var. flor de mayo, Phaseolus vulgaris and tepary beans Phaseolus acutifolius were grown under two water conditions (irrigation and drought), and four levels of urea. Vertical electrophoresis and spectrophotometric techniques were used to evaluate protein patterns, glutamate dehydrogenase (GDH), proline oxidase (PO) and pyrroline-5-carboxylate reductase (P5C reductase) enzyme activities. These enzymes were studied because they are directly related to protein synthesis. Electrophoretic patterns showed more proteins in tepary beans than in common beans with limited irrigation. GDH showed only one isozyme, with a molecular weight between 240 to 270 kDa. A decrease in PO activity was observed in common beans under drought stress with a value of 237 mol/min, in comparison to irrigation conditions of 580 mol/min. GDH and P5C reductase enzymes have had higher activity in common beans than in tepary beans under water stress. There was a significant difference only in glutamate dehydrogenase enzyme with respect to urea level. The results suggest that drought tolerance of tepary beans is due to biochemical processes related to proline metabolic enzymes.     

Preparation and antinutritional characteristics of dry bean (Phaseolus vulgaris L.) protein concentrates

Protein concentrates and starches were prepared by a wet extraction process from five dry bean (Phaseolus vulgaris L.) cultivars. The protein contents ranged from 69.7–76.4%. Concentrates prepared from dehulled beans under similar conditions had higher protein contents (80.6–87.9%). Each additional washing of the concentrates with distilled water increased their protein content. However, the protein recovery progressively decreased. The yield of starch ranged from 48.0–51.1% of the starting material. The solubility of bean proteins was minimal at pH 4.0, and under alkaline conditions, it was influenced by the tannin contents of the concentrates. Protein concentrates had lower trypsin, chymotrypsin, and amylase inhibitory activities as well as lower phytic acid and tannin contents compared to whole bean flours.     

Light and plant growth‐promoting rhizobacteria effects on Brachiaria brizantha growth and phenotypic plasticity to shade

This is the first report on the effect of light intensity and plant growth‐promoting rhizobacteria (PGPR) on the growth of a tropical forage grass, being a relevant study to improve pasture management in conventional farming and integrated crop‐livestock‐forestry systems. In this study, our aim was to evaluate the effects of light intensity and Burkholderia pyrrocinia and Pseudomonas fluorescens inoculation on Brachiaria brizantha cv. BRS Piatã growth, and phenotypic plasticity to shade. The experiment was conducted in a semi‐controlled environment. Seedlings of B. brizantha were allocated to full sun and shade. P. fluorescens and B. pyrrocinia were inoculated individually or co‐inoculated by soil drench, 14 days after seedling emergence. We evaluated morphogenesis, structural and growth parameters. Irrespective of the light regime, co‐inoculated plants had greater leaf area and SPAD index (chlorophyll content). Increase in total biomass production in co‐inoculated plants was over 100% and 300%, under full sun and shade respectively. Co‐inoculated P. fluorescens and B. pyrrocinia increased shade tolerance in B. brizantha, improving plant performance. Co‐inoculation promoted growth in B. brizantha under both sun and shade, indicating its potential as a bio‐fertilizer in conventional and integrated systems, especially in silvopastoral systems, where light availability to pasture growth may be limited.     

Interrelationships between certain physical and chemical properties of dry bean (Phaseolus vulgaris L.)

The interrelationships among certain physical and chemical properties of ten cultivars of dry beans (Phaseolus vulgaris L.) were investigated. The length/breadth ratio was similar (1.51–1.65) except for kidney beans (>2.0) and sanilac (1.37) cultivars. The breadth/thickness ratio ranged from 1.17–1.65. The 100-bean weight indicated a wide variation of 15.03–50.33 g. The density, bulk density, and porosity characteristics were within a narrow range of 1.18–1.36 g/cc, 68–75 g/100 cc, and 40.7–48.5%, respectively. Water uptake rates during the first 6 h of soaking at room temperature (21 °C) were characteristic of the cultivar. At the end of 24-h soaking, however, all cultivars had absorbed similar amounts of water (approximately 1 g/g bean). Leaching losses (g solids leached/100 g beans) had characteristic trends and ranged from 0.54 for cranberry to 3.46 for sanilac cultivars after 24-h soaking. Most correlations between selected chemical constituents and physical characteristics of the dry bean were relatively low.Journal Paper No. 2751 of Utah Agricultural Experiment Station and a contribution of Western Regional Project W-150.     

Narrow row planting increases yield and suppresses weeds in common bean (Phaseolus vulgaris L.) in a semi-arid agro-ecology of Nyagatare,Rwanda

Narrow row planting has potential to increase crop growth and yield by increasing radiation interception (RI) and minimizing intra-specific competition in the crop. It reduces weed growth and competitiveness, making resources that are normally taken up by weeds available for crop uptake. The objective of this study was to assess the effect of row spacing on weed biomass, bean growth and yield in a semi arid agro-ecology at Nyagatare, Rwanda. The study was set up as a randomized complete block design in October–December 2009 and repeated in 2011. Planting patterns at a constant bean population density of 111 000 plants ha⁻¹ random planting (normal practice), narrow row planting (30 cm × 30 cm), medium row planting (45 cm × 20 cm) and wide row planting (60 cm × 15 cm) were treatments tested in this study. The narrow row square planting pattern significantly (P < 0.01) out-yielded the wide and random planting patterns by 22–31% in the wet 2009 season and by 27–70% in the dry 2011 season. Bean plant dry weight (P < 0.01) and number of pods per plant (P < 0.01) was highest in the narrow row and lowest in the random planting pattern in the dry 2011 season. Bean plant dry weight was not significantly affected (P > 0.05) in the wet 2009 season but number of pods plant⁻¹ (P < 0.001) was highest in the narrow row and lowest in the random planting pattern. Weed biomass was significantly lower (P < 0.05) in the narrow row and the random than in the medium and wide row planting patterns at 3, 6 and 9 weeks after emergence in 2009, but the random planting had the highest weed biomass in 2011. The results suggest that the effects narrow row planting in alleviating the negative impact of inter- and intra-specific competition were more strongly expressed in the dry 2011 season than the wet 2009 season when water was probably not a limiting factor to crop growth and yield. The results also indicate that narrow and equidistant planting has potential to increase bean yield by 30%–70%, when compared to random planting (normal practice) while at the same time suppressing weed growth and is recommended for smallholder farmers in Rwanda and other semi-arid areas in sub-Saharan Africa.     

In vitro and in vivo studies on the digestibility of the major storage protein of the navy bean (Phaseolus vulgaris)

Fraction G1, the major storage protein of the navy bean (P. vulgaris), was subjected to in vitro digestion with pepsin, trypsin, or chymotrypsin. Based on measurements of the release of free amino groups, G1 appeared to be markedly resistant to digestion unless subjected to heat treatment. Molecular weight determinations by sedimentation equilibrium and amino acid analysis, however, indicated that G1 had in fact undergone limited proteolysis and a reduction in size, from 140,000 to 120,000, as a result of exposure to either trypsin or chymotrypsin. Disc gel electrophoresis in the presence of sodium dodecylsulfate also revealed significant changes in subunit composition. Native G1 was composed of two subunits, 43,000 and 49,000, in a ratio of 1:2, whereas trypsinor chymotrypsin-modified G1 had subunits of 22,500 and 30,000, in a ratio of 4:1.The true digestibility of native G1 as measured in rats was 57% compared with 92% after heat treatment. Although virtually devoid of trypsin inhibitory activity, G1 caused a significant enlargement of the pancreas, an effect which was partially overcome by heat treatment. It is concluded that a major factor contributing to the poor nutritive value of unheated navy beans is the marked resistance of its major storage protein to intestinal proteolysis.Supported by grant AM 18324 from the National Institutes of Health.     

Effectiveness of saccharin and ulvan as resistance inducers against rust and angular leaf spot in bean plants (Phaseolus vulgaris)

The present work aimed to study the effect of saccharin and ulvan against rust in artificially inoculated plants and against both rust and angular leaf spot (ALS) in naturally infected bean plants. In the greenhouse, bean plants of cv. Guará (susceptible) and FT-line (rust resistant) at first trifoliate leaf stage were sprayed with saccharin (0.24 mg mL⁻¹) or ulvan (1 or 10 mg mL⁻¹), and inoculated with Uromyces appendiculatus six days after spray. Both compounds provided an intermediary level of protection. They reduced at a similar extent the number, diameter and particularly sporulation of rust pustules in susceptible plants, suggesting that mechanisms of resistance operate at latter stages of fungus colonization. Three follow-up experiments were carried out under natural infection conditions during the spring 2009 and 2010, and summer/autumn 2010. ALS was observed during all three outdoor pot experiments, whereas rust occurred only during the spring 2009 and 2010. Saccharin sprayed every 14 days was able to protect bean plants against rust during the three experiments, whereas significantly reduced ALS only during summer/autumn and spring 2010. On the other hand, ulvan at a reduced dose rate (1 mg mL⁻¹) did not affect significantly the epidemiological development of both diseases. Increase in seed weight was observed when saccharin-treated plants were infected after pre-flowering, but not when rust infection occurred earlier. Possible reasons why such trade-offs can be obtained in certain circumstances are discussed.     

Differences in development of common bean (Phaseolus vulgaris L.) crops and pod fractions within a crop: I. Seed growth and maturity

Two experiments with common bean cultivars Rosecoco and Mwezi Moja aimed at increasing insight into differences in development with time of seeds from pods of different earliness classes within a crop. Pods were divided into three earliness classes (early, medium, and late) in relation to the fourth class, the overall crop. In all pod classes, cultivars and seasons, seeds achieved physiological maturity (PM: maximum dry weight) when fresh weight was at its maximum. PM was achieved at 58% seed moisture content in seeds from all pod classes and cultivars. Seeds from earlier pods tended to reach PM earlier than those from other classes. The time when seeds developed their final red purple colour pattern was a reasonable indicator of PM, though not completely accurate. Harvest maturity (HM: 20% seed moisture content) was reached earlier in earlier pods in cv. Rosecoco, but the timing was the same for all pod classes in cv. Mwezi Moja. The period for seeds to dry down from 58 to 20% moisture content was longer in seeds from earlier pods in cv. Mwezi Moja, but not in cv. Rosecoco. The course of decline in moisture content between 58 and 20% differed between pod earliness classes, but was not systematically affected by earliness. The timing of seed development in the overall crop could be explained by the timing of seed development in pods of different earliness classes. Relationships among seed moisture content and seed or pod colour in whole crops differed from those in pods of individual earliness classes.     

Effects of Zn rates and application forms on protein and some micronutrients accumulation in common bean (Phaseolus vulgaris L.)

In order to investigation the effects of Zn rates and application forms on protein and element contents (Fe, Cu, Mn, N and Zn) in bean plant, an experiment was conducted as a factorial based on completely randomized design at greenhouse conditions during 2006. Treatments were included zinc rates in 4 levels (10, 20, 30 and 40 mg Zn kg(-1) soil(-1) in ZnSO4 source) and 3 application forms (soil application, seed pelleting and foliar spraying). Results showed that Zn rates had significant effect on accumulation of Zn, Cu, Mn and N in bean leaves. Nitrogen accumulation in leaves reduced with increasing of zinc in the soil. Among Zn application forms, spray application had the highest accumulation of Fe, Zn and Mn in leaves (423.17, 282.89 and 88.17 mg kg(-1), respectively). The highest Zn content in seed was observed in 20 and 40 mg Zn kg(-1) soil(-1) levels (46.39 and 45.62 mg kg(-1), respectively). Meanwhile, all treatments of Zn (both rate and application) had not significant effects on Cu and Mn accumulation in bean seeds. According to interaction effects between Zn rates and application forms, the highest Fe content in seed was observed when 40 mg kg(-1) soil(-1) of Zn was applied as foliar spraying. The seed protein content nearly was stable while Zn levels was increased from 20 to 40 mg Zn kg(-1) soil(-1). Grain yield had significant correlation to zinc and Cu of leaves but, biomass had significant and negative correlation to Zn content of leaves at p < 0.05% probability levels.     

Effects of sowing methods and seeding rates on growth and yield characteristics of phasey bean (Macroptilium lathyroides)

This study was conducted to investigate the effects of sowing methods (broadcast or row sowing) and seeding rate (0.5, 1.0, 2.0 or 3.0 g/m²) on growth, yield characteristics, and forage quality of phasey bean (Macroptilium lathyroides (L.) Urb.) cv. “Murray”). Experiments were carried out in 2002 and 2003. Sowing method affected dry‐matter yield (DMY): broadcast sowing resulted in higher DMY than row sowing in 2002, and during the first harvest in 2003. Dry‐matter digestibility (DMD) and crude protein (CP) concentration were not influenced by the sowing method, except when DMD and CP of the broadcast sowing treatment increased as the plant weight increased. Contrary to expectation, seeding rate did not affect leaf area index, DMY, DMD, CP, digestible dry‐matter yield or CP yield. Even the lowest seeding rate did not influence the yield and forage quality of phasey bean, compared with the recommended higher seeding rate.     

Effect of plant density on stolon growth and development of contrasting white clover (Trifolium repens) varieties and its influence on the components of seed yield

Plants of two contrasting white clover varieties (cv. Aberystwyth S184 and Olwen) were planted in the field in spring in each of 2 years at four densities (9, 25, 49 and 100 plants m⁻²) in 1-m² plots. The effect of plant density on stolon growth and development and the components of seed yield was subsequently measured.
Stolon growth and development was influenced by plant density, variety and year. At low plant densities both white clover varieties produced longer primary stolons than at higher densities. Plant density, however, had no significant effect on the number of inflorescences at harvest. At the high stolon densities there were significantly fewer reproductive nodes per primary stolon than at the lower plant densities. Plant density did not significantly affect any other seed yield components, but the number of inflorescences at harvest, florets per inflorescence, seed set per floret and 1000-seed weight were all significantly influenced by both variety and year.
The relationship between the vegetative and reproductive growth of white clover is discussed in relation to plant density, variety and climate and the possible role of defoliation managements on inflorescence development.     

Denaturation by heat,sodium dodecyl sulphate and dithiothreitol of globulins and phaseolin from dry bean (Phaseolus vulgaris L.)

The electrophoretic pattern of the untreated common bean globulin cv. Flor de Mayo had 7 protein fractions ranging from 620 to 120 kilodaltons (kd). The last molecular weight corresponds to the monomeric form. One of the objectives of the present work was to establish a comparison among denaturation by heat, sodium dodecyl sulphate (SDS) and dithiothreitol (DTT). At pH 6.0, two bands were resistant to heat treatment, after SDS treatment of the untreated globulin several bands disappeared and two new bands with 26 and 15 kd appeared. DTT did not change the electrophoretic pattern, due to the small quantity of free SH groups in the globulin. At pH 6.0, phaseolin is partly resistant to heat denaturation due to aggregation in an acidic environment. Isoelectrofocusing (IEF) and SDS polyacrylamide gel electrophoresis (SDS-PAGE) separated phaseolin into 10 protein fractions. The hypothesis is that phaseolin or globulin aggregation is due to the charge difference of fractions.     

Increases in root yield and changes in growth of table beet (Beta vulgaris L. ssp. vulgaris var. conditiva) after treatment with chlormequat (CCC)

Field experiments in two successive seasons (1971–1972) were carried out to examine the effect of spraying table beet plants with chlormequat (CCC) on their growth and yield. Concentrations used were 500 and 1000 ppm. Spraying in the first season was carried out 60, 75 or 90 days after sowing. According to the result of the first season, which showed no significant differences according to varying spraying date, only one spraying time was chosen for the second season (75 days).In both seasons, CCC exhibited modifications in growth and increased root yield significantly. Number of leaves and their dry weight per plant were increased. The increases in leaves dry weight per plant was found not to be a result of the increase of leaves number per plant only, but the leaves dry weight percentage was also increasedRoot length showed insignificant increases due to CCC treatment. In the same time, significant increments in its diameter as well as in dry weight per plant were recorded in both seasons, which might be the cause of increasing yield. Ratio of leaves/roots (dry weight) was shifted in the treated plant towards the root side.

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Zusammenfassung In den Jahren 1971 und 1972 wurden zwei Feldversuche angelegt, um den Effekt der CCC Spritzbehandlung auf das Wachstum und den Ertrag von Roten-Rüben zu prüfen. Es wurden Konzentrationen von 500 und 1000 ppm angewandt. Im ersten Jahr erfolgte die Spritzung zu drei Terminen: 60, 75 oder 90 Tage nach der Aussaat.Auf Grund der Ergebnisse des ersten Jahres, in dem die Spritztermine keinen Einfluß auf die Wirkungen der CCC Behandlungen hatten, entschied man sich im zweiten Jahr für nur einen Spritztermin und zwar 75 Tage nach Aussaat.In beiden Jahren beeinflußte die CCC Behandlung das Wachstum und steigerte signifikant den Ertrag. Sowohl die Zahl der Blätter als auch deren Trockengewicht pro Pflanze erhöhten sich. Die Zunahme des Trockengewichts hing nicht nur mit der größeren Anzahl der Blätter pro Pflanze zusammen, sondern auch mit dem prozentualen Anstieg des Trockengewichts nach CCC Behandlung.Die Wurzellänge zeigte keine signifikante Zunahme in Abhängigkeit von der CCC Behandlung, während sich der Wurzeldurchmesser ebenso wie das Trockengewicht pro Pflanze signifikant in beiden Jahren erhöhte, worin der Grund für die Ertragssteigerung zu suchen sein wird. Das Verhältnis der Trockengewichte Blätter/Wurzeln verschiebt sich in den behandelten Pflanzen zu Gunsten der Wurzeln.

     

不同植物激素对油菜角果生长和结实的影响

利用不同植物激素溶液在结角期间处理油菜角果,结果对油菜角果生长和结实均有较好影响,其中1.5mg/L 激动素 1mg/L萘乙酸混合液处理1次,对角果增长增粗和每角粒数增加作用较大,1.5mg/L激动素处理2次和3次对粒重增长作用较大,1mg/L萘乙酸处理2～3次对提高种子含油量作用较大.