Rootstock Induced Seasonal Changes of N,P and K Nutrient Levels in Satsuma Mandarin cvs. ‘Okitsu’, ‘Clausellina’ and ‘Silverhill’

Rootstocks-scion relations have critical consequences about nutritional status, tree vigour, resistance to pest and pathogens, yield and yield quality. In this study, we therefore investigated seasonal variations of leaf mineral nutrient concentrations in Satsuma mandarin’s cvs. ‘Okitsu’, ‘Clausellina’ and ‘Silverhill’ budded on different citrus rootstocks under Dörtyol-Hatay, Turkey ecological conditions. Nitrogen (N), phosphorus (P) and K (potassium) concentrations of the leaves showed slightly similar trend each other, in general these nutrients reduced from January to March–April, and increased during the growing season until mid- and late summer, then decreased until December. Despite rootstocks did not significantly affect the leaf N, P and K concentrations rootstock-scion relation was pre-eminent and nutrient dependent. Most conspicuous scion dependent changes were recorded for K concentration whereas P concentration was little influenced. In terms of scion-rootstock combination, the range of N and K concentrations were 2.11% (‘Okitsu’/sour orange) to 2.34% (Silverhill/sour orange), and 1.09% (‘Okitsu’/Carrizo citrange) to 1.39% (‘Clausellina’/Carrizo citrange), respectively; but P concentrations were highly similar %0.13 (‘Okitsu’/sour orange) – %0.16 (‘Clausellina’/Carrizo citrange), in the entire growing season. It can be concluded that there are considerable differences in nutrient uptake ability of scion-rootstock combination.     

Day and night temperatures,daily light integral,and CO2 enrichment affect growth and flower development of Campanula carpatica ‘Blue Clips’

Campanula carpatica Jacq. ‘Blue Clips’ plants were grown in a greenhouse under nine combinations of day and night temperatures created by moving plants every 12 h among three day/night temperatures (15, 20, and 25°C). At each temperature, there were three daily light integrals (DLI; 4.2, 10.8, and 15.8 mol m⁻² per day, averaged over the experimental period) created with varying supplemental light, and ambient (≈400 μmol mol⁻¹) and enriched (≈600 μmol mol⁻¹) CO₂ concentrations. Time to flower was closely related to average daily temperature (ADT), and was not significantly affected by the day or night temperatures delivered to achieve a specific ADT. Time to flower was not largely affected by DLI or CO₂ enrichment. As plant ADT increased between 15 and 25°C, flower diameter decreased about 1 mm per degree and was not related to the difference between day and night temperatures (DIF). Flower diameter was smallest and least sensitive to changes in temperature at lower DLI and at ambient CO₂ levels. There were 10 less flower buds and 0.3 g less dry mass per plant at first flower for every 1° increase in plant ADT at high and medium DLIs. Flower bud number and dry mass were relatively low and less sensitive to changes in ADT at low DLI, and increased slightly with CO₂ enrichment at medium and high but not at low DLI. Plant height was not related to ADT, but increased linearly as DIF increased from −6 to 12°C at all DLIs, but the response was stronger under low DLI than high and medium DLIs. Flower bud number and dry mass were correlated closely with the ratio of DLI to daily thermal time (base temperature of 0°C). Flower bud number and dry mass were highest when C. carpatica plants were grown at 15°C with a DLI of 10–15 mol m⁻² per day.     

Effects of 22S, 23S-Homobrassinolide and Gibberellic Acid on Occurrence of Physiological Disorders and Fruit Quality of ‘Summit’ and ‘Regina’ Sweet Cherries

Plant growth regulators play a vital role in fruit growth and development. However, effects of compounds belonging to the group of brassinosteroids have not been fully investigated in sweet cherries. One relatively new and one commonly applied growth regulator, 22S, 23S-Homobrassinolide and GA₃, respectively, were tested on ‘Summit’ and ‘Regina’ sweet cherries to determine the effects on fruit quality and physiological disorders. The substances were applied with a handgun sprayer at full bloom and at the beginning of fruit development (shuck split) for a 2-year period. GA₃ was applied at the concentrations of 25, 50, 100 mgL^?1 and 22S, 23S-Homobrassinolide at 0.05, 0.1, 0.5 mgL^?1. A combined application of 100 mgL^?1 GA₃ + 0.1 mgL^?1 22S, 23S-Homobrassinolide was also applied. Effects of GA₃ and 22S, 23S-Homobrassinolide on fruit weight and size were evident for the concentrations applied and between the seasons for both of the cultivars. Combination of the both growth regulators and 100 mgL^?1 GA₃ alone produced longer fruits compared to the control trees in ‘Regina’. The 22S, 23S-Homobrassinolide applications increased the firmness of the flesh slightly, but not to a great degree, compared to the GA₃ applications alone. The brightness of the red color was better with GA₃ applications in ‘Summit’ and ‘Regina’. Brassinosteroid caused the fruits to have comparably dull red color. Total soluble solid contents of the cultivars were influenced by the substances and the year of the application. Stem resistance to separation from the fruit was under the influence of both treatments and seasons. In general, 22S, 23S-Homobrassinolide provided better stem resistance than the GA₃ applications. There were no effects of the substances on the development of physiological disorders.     

Effects of clonal rootstocks on ‘Hass’ avocado yield components,alternate bearing,and nutrition

Summary

Growth, yield, and leaf nutrient concentrations were measured in ‘Hass’ avocado (Persea americana Mill.) trees grown on one of ten clonally-propagated rootstocks (‘Borchard’, ‘D9’, ‘Duke 7’, G1033, G755A, G755B, G755C, ‘Thomas’, ‘Topa Topa’, or ‘Toro Canyon’) over a 10-year period in southern California. After 10 years, trees on ‘Borchard’ were larger than trees on all other rootstocks. Trees on all rootstocks displayed an alternate-bearing pattern, typical of avocado. Alternate-bearing was most pronounced in trees grafted onto ‘Topa Topa’ and ‘Toro Canyon’. Rootstocks in the G755 series had the lowest alternate-bearing index, but also had the lowest yields.Trees on ‘Duke 7’ and ‘Borchard’ had the highest cumulative yields, and trees on G755A, G755B, and G755C had the lowest yields. Differences in yield were due to differences in the number of fruit per tree, not individual fruit weight. When yield was evaluated in terms of canopy efficiency (kg fruit m^–3), no rootstock outperformed ‘Duke 7’, the industry standard rootstock. Leaf concentrations of all nutrients examined (N, P, K, Ca, Mg, S, Zn, Cl, Mn, B, Fe, and Cu) were within, or close to the recommended ranges. P, Ca, and S were higher, and Fe was lower in high-yielding years in all rootstocks.     

Secondary school students’ perspectives and use of three school grounds of varying size,content and design

School grounds can have multiple values, and especially sufficient size, green elements, variation, integrated design and participatory development have been described as positive qualities. However, many studies have focused on pre-school and primary school grounds, while less is known about school grounds for adolescents. This study explored how secondary school students experience and use school grounds of varying size, content and design. The study included one class in year 8 (aged 14–15) at each of three schools in southern Sweden. A questionnaire with mainly open questions was distributed, followed by both individual interviews and walking interviews with four students from each class. The results show that school grounds were appreciated, but also that many chose to stay indoors. Large surface area and varied content, with ball courts, greenery, seating and multifunctional equipment, were valued, but a school ground design with integrated and pleasant settings, allowing socialising and activities, particularly support use by secondary school students.     

Inflorescence bud growth,development and abscission in shoots of bearing and disbudded ‘Bianca’ pistachio trees

Summary

Dry-matter accumulation patterns and anatomical aspects of development and abscission of inflorescence buds borne on shoots of fruiting (BF) and non-fruiting (BNF) branches of bearing trees and in non-bearing branches of disbudded (NBD) pistillate trees of pistachio (Pistacia vera L.) were studied from the end of May to mid September. In NBD trees, inflorescence-bud summer drop was negligible and, by the end of the growing season, dry-matter content in NBD buds was three times higher than in BF buds. The anatomical investigation showed that the inflorescence buds, irrespective of their being on shoots of fruiting or not-fruiting branches, undergo the same differentiation process, which is completed by the end of spring. In June and July, signs of degenerations are detectable in the conducting tissue of the buds that will abscise. The degeneration seems to precede and, presumably, trigger the onset of abscission layers, which may develop in different points of the inflorescence. It is suggested that the event triggering the onset of the abscission process precedes the phase of rapid embryo development and that it might be ascribed to the embryo in the phase following the first zygotic division.     

Calcium and Potassium Foliar Sprays Affect Fruit Skin Color,Quality Attributes,and Mineral Nutrient Concentrations of ‘Red Delicious’ Apples

This study was conducted to evaluate the influences of foliar spray of potassium (K), calcium (Ca), and their combination on the fruit red skin color and quality attributes of ‘Red Delicious’ apple under conditions of south central Iran with warm and dry summer, where low and high temperature differences are low. The trees were sprayed five times using 5 g L^–1 calcium chloride (CaCl₂) at 3-week intervals starting from 3 weeks after full bloom and three times (at 9, 12, and 15 weeks after full bloom) using 2.5 g L^–1 K sources [potassium chloride (KCl), potassium sulfate (K₂SO₄), and potassium nitrate (KNO₃)] during two growing seasons in 2013 and 2014. Anthocyanin, some physicochemical attributes, and fruit mineral concentrations were measured at harvest. Results showed that spraying with K, CaCl₂, and their combinations significantly increased fruit weight, sugar and anthocyanin concentrations, firmness, and K uptake. A combined foliar application of CaCl₂ and each of the K sources was more effective on the improvement of fruit color, firmness, fruit K and Ca uptake, and K/Ca ratio as compared to the case when either K or Ca was applied alone.     

Effect of agar,galactomannan and indole-butyric acid on in vitro rooting of the pear cultivar ‘Durondeau’ and apple rootstock cultivar ‘Marubakaido’

Summary

The influence of partial substitution of agar by galactomannans in culture media supplemented with different concentrations of indole-3-butyric acid (IBA) was studied on in vitro rooting of pear (Pyrus communis L.) cultivar ‘Durondeau’ and apple rootstock (Malus prunifolia Borkh.) cultivar ‘Marubakaido’. The galactomannans applied were obtained from Cassia fastuosa (cassia) and Cyamopsis tetragonolobus (guar gum) seeds. The results obtained with mixtures of agar and galactomannan (3 g l^–1 each) were compared with those from media solidified with a standard concentration of agar (6 g l^–1). The rooting of pear shoots was enhanced significantly in the presence of a mixture of agar plus cassia galactomannan compared to medium solidified with agar only. The modified media promoted a higher number of roots than the control, and increased the percentage of rooted shoots. A maximum of 84.8% rooting was obtained on half-strength MS medium (¹?2MS) supplemented with 0.49 µM IBA and solidified with a blend of agar plus cassia galactomannan. For the apple rootstock, only the number of roots per shoot was influenced significantly by the addition of galactomannan to the rooting medium. The highest number of roots per shoot was 16.67 on ¹?2MS medium gelled with a mixture of agar plus guar galactomannan supplemented with 4.90 µM IBA. The behaviour of the agar-galactomannan gel and the possibility of reduced costs when compared with systems containing only agar, suggest new biological and commercial applications for galactomannans.     

Interaction of Crop Load and Irrigation on Yield,Fruit Size,Color and Stem-end Splitting Ratio of Apple c.v. ‘Gala,Galaxy’

In this study, the interaction between crop load and irrigation level on yield, fruit size, skin color and stem-end splitting fruit ratio in the apple cultivar ‘Gala, Galaxy’ grafted on rootstock M9 were investigated. Six irrigation programs were applied during the whole growth season: deficit irrigation (rates of 0.25 kc, 0.50 kc, 0.75 kc), full irrigation (rate of 1.00 kc), excess irrigation (rate of 1.25 kc) and non-irrigation (rates of 0.00 kc of “Class A” pan evaporation coefficient). Four crop loads in each irrigation application were performed by hand thinning after the June drop as a- a low crop load (3 fruits cm^?2 TCA), b- a medium crop load (5 fruits cm^?2 TCA), c- a heavy crop load (7 fruits cm^?2 TCA), and d- an un-thinned crop load (>?7 fruits cm^?2 TCA). The total tree yield increased with crop load and irrigation levels. Fruit size was significantly increased by the low crop load. Irrigation increased the fruit size compared to non-irrigation treatment. Further 0.75 kc, 1.00 kc and 1.25 kc irrigation treatments significantly increased the fruit length. Irrigation reduced the fruit flesh firmness. While the low crop load increased the skin red color, it decreased the fruit skin brightness. The yellowness of skin decreased with increasing in the irrigation amount. Irrigation reduced the skin brightness and yellowness, but it increased red color. Crop load and irrigation significantly affected the stem-end splitting fruit ratio. While the splitting fruit ratio increased with a decrease in the crop load, it decreased with an increase in irrigation amount, relatively. Consequently, the low and medium crop load treatments would be beneficial to increase the ratio of marketable fruits without any significant losses in yield for ‘Gala’ apple, especially under 0.75 kc deficit irrigation treatment.     

Peoples’ use of,and concerns about,green space networks: A case study of Birchwood,Warrington New Town,UK

Cultural services provided by green space networks and in particular leisure and recreational opportunities are central to the quality of life of those living in urban areas. However, the literature concerned with green space networks has mainly focused on planning aspects rather than on recreational use. The aim of this study was to evaluate the recreational use of, and concerns about, a naturalistic green space network. The case study location was the naturalistic woodland framework in Birchwood, Warrington, UK, known as Birchwood Forest Park. Non-participant observation and content analysis of local archives were used to collect quantitative and qualitative data. Birchwood Forest Park was used more for leisure activities (52.8%, N=1825; i.e. recreation, sports or play) than for utilitarian purposes (47.2%, N=1825; i.e. as walking or cycling thoroughfare). However, utilitarian walking (30%, N=1825) was the most frequent type of activity observed. The maintenance of the naturalistic woodland framework was the most frequent concern mentioned in the local archives (33.3%, N=234). This case study suggests that the recreational patterns in, as well as peoples' concerns about, naturalistic urban landscapes may be a factor of high-quality maintenance and associated local aesthetic and cultural perceptions. In developing, planning or managing comprehensive urban green space networks it is important to ensure that natural looking scenes are well maintained and that the local community is culturally connected to such scenes.     

Fruiting position,mineral concentration and incidence of physiological pitting in ‘Hayward’ kiwifruit

Summary

‘Hayward’ kiwifruit (Actinidia deliciosa (A. Chev) C. F. Liang et A. R. Ferguson) were sampled to identify populations of fruit with differing fruit mineral concentrations and levels of the storage-related disorder physiological pitting. Fruit were taken from different shoot types at different locations within vines. Fruit from short shoots near the tips of canes, in an area of the vine with low leaf:fruit ratios, had low fruit calcium concentrations and more pitting. In contrast, fruit from long shoots and with high leaf:fruit ratios near the base of canes, had high concentrations of calcium and less pitting. At the more distal positions along canes in an area of the vine with low leaf:fruit ratios, increasing leaf:fruit ratios on individual fruiting shoots led to higher inflow of calcium to the fruit. No such relationship was found when fruit were sampled from shoots near the base of canes, an area of the vine with relatively high leaf:fruit ratios. Inconsistent relationships were found between fruit soluble solids concentrations and calcium, probably due to different mobility of calcium and carbohydrates within the vines. Fruiting position and the associated leaf area are a source of variability in mineral concentrations of fruit, and by consequence, in the incidence of physiological pitting in ‘Hayward’ kiwifruit, and should be considered when developing sampling techniques.     

Influence of habitat amount,arrangement, and use on population trend estimates of male Kirtland’s warblers

Kirtland’s warblers (Dendroica kirtlandii) persist in a naturally patchy environment of young, regenerating jack pine forests (i.e., 5–23 years old) created after wildfires and human logging activities. We examined how changing landscape structure from 26 years of forest management and wildfire disturbances influenced population size and spatial dispersion of male Kirtland’s warblers within their restricted breeding range in northern Lower Michigan, USA. The male Kirtland’s warbler population was six times larger in 2004 (1,322) compared to 1979 (205); the change was nonlinear with 1987 and 1994 identified as significant points of change. In 1987, the population trend began increasing after a slowly declining trend prior to 1987, and the rate of increase appeared to slow after 1994. Total amount of suitable habitat and the relative area of wildfire-regenerated habitat were the most important factors explaining population trend. Suitable habitat increased 149% primarily due to increasing plantations from forest management. The relative amount and location of wildfire-regenerated habitat modified the distribution of males among various habitat types, and the spatial variation in their abundance across the primary breeding range. These findings indicate that the Kirtland’s warbler male population shifted its use of habitat types temporally and spatially as the population increased and as the relative availability of habitats changed through time. We demonstrate that researchers and managers need to consider not only habitat quality, but the temporal and the spatial context of habitat availability and population levels when making habitat restoration decisions.     

Culture method and photosynthetic photon flux affect photosynthesis,growth and survival of Limonium ‘Misty Blue’ in vitro

Microcuttings (shoots each with two leaves) of Limonium ‘Misty Blue’ were cultivated in vitro for 28 days under photoautotrophic (sucrose-free culture medium; CO₂ and photosynthetic photon flux (PPF) enriched conditions), photomixotrophic (medium with 30 g l⁻¹ sucrose; CO₂ and PPF enriched conditions) and heterotrophic (medium with 30 g l⁻¹ sucrose; CO₂ non-enriched conditions) methods. Several growth variables were measured during and at the end of cultivation: shoot fresh and dry weight, percentage of shoot dry matter, root fresh weight, number of leaves, leaf area, chlorophyll and sugar content of leaves, stomatal density and size, net photosynthetic rate (NPR) and percent survival of plantlets ex vitro. Plantlets grown in photoautotrophic and photomixotrophic methods had more leaves, high chlorophyll and sugar contents, high NPR, and showed high percent survival. However, these plantlets possessed less number of stomata per square millimeter. In contrast, the plantlets grown by the heterotrophic method showed decreased values of these growth variables except for the number of stomata per square millimeter. These results indicate that CO₂ enrichment for plantlets in vitro at a relatively high PPF would promote photosynthesis and hence growth of chlorophyllous explants/plantlets in vitro. The resulting plantlets were acclimatized better and sooner on ex vitro transplantation.     

Determination of Salt Tolerance of ‘Camarosa’ and ‘Chandler’ Strawberries Grown in Perlite and Perlite

ABSTRACT

The effects of salt (NaCl) applications of various concentrations (0, 500, 1000 and 2000 mg/L) for 6 months on ‘Camarosa’ and ‘Chandler’ strawberry varieties grown in perlite and perlite:zeolite (1:1) media were investigated. In order to determine the salt resistance of varieties, the tolerance index (T.I.) and tolerance ratio (T.R.) were calculated on the basis of total chlorophyll content, and leaf and root dry weight. Also, the ability of keep in balance for potassium:sodium (K:Na) ratio in the aerial part and roots of plants were considered. It was determined that the highest T.I. and T.R. values calculated on the basis of leaf and root dry weight in cv. Camarosa in both media. Concerning the evaluations calculated on the basis of total chlorophyll, the results indicated that ‘Chandler’ is more resistant in perlite medium, ‘Camarosa’ is more resistant in perlite:zeolite (1:1) medium. K:Na ratio decreased with increasing salinity in both varieties. However, it was determined that K:Na ratio is higher in cv. Chandler, i.e., 0.83 than in cv. Camarosa (0.61). As a conclusion, it was determined that ‘Camarosa’ was more resistant to salt than ‘Chandler’. In addition, regarding the salt resistant performance of plants, perlite:zeolite (1:1) medium had more effective results than perlite.     

Effects of different crop loads and thinning times on yield,fruit quality,and return bloom in Malus × domestica Borkh. ‘Elstar’

Summary

‘Elstar’ is the latest-maturing commercial apple cultivar grown in Norway, with high fruit quality when properly managed. In May 2006, an experiment with four different crop loads [2, 4 ,6, or 8 flowers or fruitlets cm^–2 trunk cross-sectional area (TCSA), respectively] was established at two different stages [first bloom (FB), or 20-mm diameter fruitlets] and compared to unthinned control trees. Fruit growth was measured on individual fruit for each treatment throughout the season at weekly intervals. Thinning at FB gave a significantly lower final percentage fruit set than thinning to the same cropping level at the 20-mm fruitlet stage. However, fruit weights and soluble solids contents (SSC) were significantly higher, and the background fruit colour improved when trees were thinned at FB. The final number of fruit at harvest was less than the amount established at FB, or at the 20-mm fruitlet stage. There were significant differences between treatments in final fruit numbers per TCSA, which reflected the different crop loads. Fruit weights and SSC values were highest with the lowest crop load, and decreased with increasing crop loads. There was also a strong crop-load effect on the extent of return bloom per tree in the subsequent year. Trees thinned at FB had significantly more flower clusters than those thinned at the 20-mm fruitlet stage of. Untreated control trees had the lowest number of flower clusters. The amount of return bloom declined with increasing crop load. Second year crop loads and fruit weights were highest when trees were thinned at FB to two or four apples cm^?2 TCSA in the previous year. Trees with the highest crop load had the lowest crop load in the following year. Fruit quality was generally high for all treatments.     

Effect of part-tree flower thinning on fruiting,vegetative growth and leaf photosynthesis in ‘Cox’s Orange Pippin’ apple

Flower clusters were removed at full bloom from ten year old ‘Cox’s Orange Pippin’ trees on M.9 rootstocks, over the whole tree, on alternate branches or on whole sides. Mean fruit weight per tree at harvest was linearly dependent on leaf area per fruit and on light interception per fruit, both relationships accounting for over 90% of the variance. These relationships did not differ between treatments, implying either a mobile pool of carbohydrate or photosynthetic adjustment within the tree to crop load. Measurements of leaf photosynthesis in July and September showed no statistically significant differences in photosynthetic rate of spur or extension shoot leaves on bearing or non-bearing branches. Although the treatments caused no overall effects on shoot growth or leaf area per tree, sides of trees without fruit had greater leaf area and shoot growth than did sides bearing fruit. Fruit mineral composition and percentage dry weight were not affected by treatment except where the treatments significantly altered fruit size. In the following spring, although the treatments did not affect the total number of flower buds produced, branches that were deflowered in the previous spring carried significantly more flower buds than did branches which had cropped.     

Thailand’s catastrophic flood: Bangkok tree mortality as a function of taxa,habitat, and tree size

Urban areas have unique environments such as high air temperature, soil compaction and disturbance, and air and water pollution often impose plant stresses. Natural disasters can impose even greater stresses on plants in such areas. In 2011 Bangkok, Thailand experienced its worst flooding in over two decades, inundated up to a meter for a month. Here we report on tree tolerance to submerged, anoxic soils based on mortality. We identified over 6500 trees by taxa: 395 species in 219 genera within 60 families, which were categorized into three groups: susceptible (>50% mortality), tolerant (<50% mortality), and highly tolerant (no mortality). Among all the species, 18% were categorized as flood susceptible, 75% as tolerant, and 7% as highly tolerant. The floods resulted in decreased overall species richness by 18%, particularly in the Magnoliaceae and Lauraceae families, for which the mortality was 100% and 66%, respectively. Flood susceptible species were mostly from high rainfall habitats such as the hill evergreen forest. As expected, highly flood tolerant species were from the mangrove forests, beach/strand forests, and swamp plant communities, where the root zones are persistently saturated with lower quality water. Unexpectedly, many species native to higher temperature, drier, often deciduous lowland habitats were found to be flood tolerant, and were from the cultivated fruit and ornamental species. The results also indicated that smaller and younger trees suffered more mortality than larger and more mature trees. Therefore, during the tree selection process for planting in urban environments that may be at risk of frequent flooding, species from a wide spectrum of ecological habitats should be considered; particularly those characterized by one or more environmental stresses, such as drought, salt, heat, or saturated soils.     

The effect of summer pruning and nitrogen on growth,cropping and storage quality of Cox’s Orange Pippin apple

Pruning in late summer was compared with winter pruning on mature 23-year-old spur pruned trees of Cox’s Orange Pippin on M.2 rootstock. Two nitrogen treatments were superimposed, namely a standard dressing of 126 units N (sulphate of ammonia) applied in March as compared with 288 units N (nitrate of soda) applied in mid-August at the time of summer pruning. Summer pruning reduced trunk girth increment, advanced blossom development, increased the amount of red colour on the fruits and drastically reduced the incidence of bitter pit in stored fruit. The treatments did not affect cropping. Nitrogen treatments had no overall effect upon the incidence of bitter pit, but in the first season, when bitter pit was most prevalent, high levels of N applied in August reduced the amount, a similar trend being recorded the following year.