南方欧亚种葡萄引种评价和栽培技术研究

评价了35个欧亚种葡萄品种,认为适合南方栽培的欧亚种葡萄基因型有9个,以其中2个表现优良的主栽品种为试材,研究了南方欧亚种葡萄优质稳产栽培的几个主要技术影响因子。结果表明,矢富罗莎、红地球分别选择SO4、巨峰作砧木,生长结果最优,平均粒重较自根苗分别增加21.6%、10.7%,可溶性固形物含量分别提高14.8%、2.8%;2个品种采用单十字飞鸟型架式较双十字V型架产量分别提高20.7%、15.0%;2个品种采用5-4-3-2-1摘心法较常规方法结果枝率分别提高51.9%、28.6%;使用细胞酶制剂叶面肥粒重增加1.2￣1.6g,可溶性固形物含量提高1%,对降低果实酸度也有一定的影响,明显改善红色品种果实外观。     

Application timing and concentration of abscisic acid affect the quality of ‘Redglobe’ grapes

Summary

The plant hormone abscisic acid (ABA), the concentration of which increases in grape berry skins at the onset of maturation (veraison), appears to be involved in the regulation of anthocyanin accumulation. Preliminary tests suggested that exogenous applications of ABA could improve grape berry colour, but its high cost precluded the development of commercial applications. Recently, a lower-cost ABA production method was developed, which led to the evaluation of different concentrations of ABA, applied at or around veraison, on the quality of ‘Redglobe’ grapes. In two of three years of tests, several ABA treatments enhanced the anthocyanin content of grape skins. ABA, applied at approx. 300 mg l^–1 at veraison, may be required to reliably increase pigmentation, and improve the colour of ‘Redglobe’ grapes. Although the total anthocyanin content was increased by ABA treatment, anthocyanin composition was not affected. Applications of ABA had few effects on fruit size or composition, although they did cause fruit softening, which is undesirable. A secondary objective of this study was to determine how the anthocyanin content of berry skins affected berry colour characteristics. Strong curvilinear relationships between anthocyanin content and lightness and hue showed that these colour characteristics were saturated by anthocyanin contents over 0.02 mg cm^–2 of skin. These data suggest that colour measurements may be needed to evaluate the effect of cultural practices on colour in table grapes.     

Stolon Development and Cultural Production Practices of Winter-Grown Strawberries

ABSTRACT

The four experiments described here are part of a 5-year program focused on determining whether day-neutral and short-day strawberries would be suitable for commercial off-season/winter (temperate climate) production in greenhouses. All research was performed using container-grown plants fertigated through a capillary mat production system with heat supplied under the benches. The first experiment focused on stolon development. The second experiment investigated the influence of stolon removal on berry production and included the removal of flowers for the first three weeks. The third and fourth experiments were conducted as a comparison of university research (experiment 3) versus a commercial operation (experiment 4). In experiment one, of 13 cultivars, ‘AC Wendy’ plants produced significantly more stolons than the other cultivars. In experiment two, stolon production peaked at weeks 12 and 13, with ‘Chandler’ plants producing significantly more stolons (starting at week 9) than the other cultivars. At week 13, ‘Seascape’ plants produced more total berry weight than the other cultivars. The influence of stolon removal on total berry weight varied, with ‘Evie-2+’ plants (large crowns) producing higher total berry weight with the stolons on as compared to ‘Evie-2’ plants, which produced higher total berry weight with the stolons removed. In experiment 2, fruit production was low and was probably a result of deflowering for three weeks. Consequently, it may not be advisable to deflower during the ‘off-season’ for greenhouse-grown strawberries. In the final two experiments, which involved a commercial cooperator as well as the university greenhouse, it was found that of the five cultivars trialed, the same cultivars at both sites produced the maximum total berry weight. Stolon production differed at the two locations, but the optimal time for stolon removal was similar. Thus, it is recommended that for winter greenhouse production, stolons be removed initially at week 8 and then at weeks 11–12 of the production cycle.     

Vegetative Growth,Productivity, Berry Quality Attributes and Leaf Macronutrients Content of Currants as Affected by Species and Cultivars

Bush vigor, berry physical traits and chemical composition, seasonal changes of leaf macronutrients content and leaf mineral status at mid-summer (120 DAFB, Days After Full Bloom) were evaluated in black, red and white currants and their cultivars which are grown on heavy soil, and with reduced cultural practices, except pruning, fertilization with cattle manure and weed control with herbicide, under western Serbian conditions. Results indicated that black currant had lower bush size, yield, bunch weight and number of berries per bunch, but had significantly higher berry weight as compared to red and white currants. Red and white currants had similar bush size, yield and bunch weight. Berries of black currant contained much higher levels of soluble solids content, acidity, vitamin C, total phenolics and flavonoids content than both red and white currants, whereas ripening index was similar. Leaf of black currants had lower macronutrients content, except leaf Mg, as compared with others. Very high excesses of all leaf macronutrients content, except in some cases for leaf N and Mg, were observed in currants in mid summer, i.?e. at 120 DAFB. Black currant showed the best balanced nutritional values, whereas red currant showed a wider imbalance in nutritional values. Beside currant species, strong effect of cultivar per se was found on the most of evaluated features. Generally, black currant cv. ‘Eva’, red currant cv. ‘Slovakia’, somewhat ‘Detvan’, and white currant cv. ‘Primus’ had higher bush size, better berry physico-chemical attributes and higher leaf macronutrients content than others. Finally, ‘Eva’, ‘Tatran’ and ‘Meridian’ showed the best balanced nutritional values at 120 DAFB.     

Effect of water deficit on Merlot grape tannins and anthocyanins across four seasons

Tannins and anthocyanins impart important sensory attributes and potential health benefits in wine. The effect of water deficits (WDs) on fruit growth, anthocyanins, and skin tannins was investigated in field grown Vitis vinifera L. ‘Merlot’ berries across four seasons (2004, 2005, 2007, and 2008) by imposing deficits from the onset of ripening until maturity. WD reduced berry weight and increased the concentration of anthocyanins all four seasons, and increased the concentration of tannins three of four seasons. Under WD, anthocyanin concentration at harvest (mg/g of berry fresh weight) was 53.0–61.6% greater than in Controls (C), and anthocyanin content (mg/berry) was 28.7–35.3% greater than in C. By contrast, WD increased tannin concentration (mg/g of berry fresh weight) at harvest by 12.6–36.9% compared to C in 2004, 2005, and 2008, and did not influence the concentration in 2007. Tannin content (mg/berry) at harvest was unaffected except in 2004 when it was 25.7% greater than in C. Thus, water deficits consistently increased anthocyanin concentration by increasing content per berry and reducing fruit growth, but increased tannin concentration less and only by reducing fruit growth, except in one year where the tannin content was increased. These results demonstrate that management of vine water deficit during ripening is a much more effective tool to increase anthocyanins than tannins in Merlot grapes.     

Fruit growth and chemical properties of Ribes magellanicum “parrilla”

The aim of this work was to study the time course and pattern of fruit growth and the evolution of some of the chemical attributes such as soluble solids, acidity, and anthocyanin content in Ribes magellanicum plants growing in a natural environment near Ushuaia city, Tierra del Fuego (Argentina). Fruit growth and composition presented significant changes along the days from the full bloom phase. Fresh and dry fruit weight exhibited a typical double sigmoid curve. The first period of rapid fresh fruit growth was from full bloom phase until 28 days after, followed by a lag period until 42 days from the full bloom phase, and then by a second period of rapid increase until 56 days from the full bloom phase. Then, fresh fruit weight increased slowly (days 70–98 from the full bloom phase), reaching its maximum. Afterwards, fresh fruit weight decreased significantly until the end of the summer, and the fruiting period approximately ended 112 days from full bloom phase. On a dry weight basis the maximum fruit biomass was reached 98 days from the full bloom phase. Evolution of fruit growth was related with the compositional changes evaluated. By day 98 from the full bloom phase, soluble solids (17.5°Brix) and anthocyanin content (240.1 mg/100 g fruit fresh weight) were at their maximum, while at this time the total titratable acidity was at a minimum (0.4%). The results obtained not only contribute to the knowledge of the quantitative content of anthocyanin, a metabolite with nutraceutical value, but also give some tools for the definition of the optimal harvest time of R. magellanicum fruits, which it is important for fruit destination.     

Soil pH effects on yield and fruit size of two rabbiteye blueberry cultivars

The effects of soil pH on yield and average berry weight of rabbiteye blueberry cvs Delite and Tifblue were investigated. Over a three year period, the higher soil pH decreased from 6.5 to 5.9, but the total yield of both cultivars decreased more than 60% as soil pH increased from 4.5 to 6.5 in the first two years and to 5.9 during the last year. Percent marketable yield was less influenced by the change in soil pH than was total yield. ‘Tifblue’ produced more fruit and had higher marketable yields, but the fruit size determined by average berry weight was smaller than ‘Delite’. When the soil pH was at a more desirable range for maximum yield (4.5 to 5.3), ripening was more uniform for both cultivars. This study illustrated that: (a) two rabbiteye blueberry cultivars differed in tolerance to alkaline growing conditions, (b) the response of the two cultivars to the change in soil pH varied from year to year, and (c) total yield of both cultivars was maximized when soil pH was below 5.0.     

Influence of soil hydrothermal environment,irrigation regime,and different mulches on the growth and fruit quality of strawberry (Fragaria × ananassa L.) plants in a sub-temperate climate

Summary

The present study was undertaken over two consecutive years under sub-temperate climatic conditions in the mid-hill region of Himachal Pradesh (30°52'N, 77°11'E; 1,175 m asl) on loamy sand Inceptisols. The aim was to investigate the effects of irrigation and mulch material on the growth, flowering, fruiting behaviour, relative leaf water content (RLWC), yield, and quality of strawberry (Fragaria × ananassa L. ‘Chandler’). The drip irrigation treatments included irrigation with 100, 80, or 60% (coded 1.0, 0.8, and 0.6 V) of the total water requirement. Both mulches increased the minimum soil temperature to a depth of 5 cm. The drip and surface irrigation treatments raised the minimum soil temperature by 3.0º – 5.4ºC, and lowered the maximum temperature by 2.2º – 5.8ºC compared to the rainfed control. Hay mulch was more effective in raising the minimum temperature and lowering the maximum soil temperature than black polyethylene mulch. Moisture conservation increased by 2.8 – 12.8% under the black polyethylene mulch compared to the no-mulch treatment. Drip and surface irrigation methods, as well as mulching, were found to be effective for enhancing the growth, irrigation water use efficiency (IWUE), fruit yield, and quality of strawberry plants. However, the number of crowns per plant, the percentage berry set, the RLWC, root length density (RLD), and fruit yield were highest under treatment M3I3 [i.e., black polyethylene mulch plus drip irrigation (1.0 V)] by 565.5%, 94.5%, 32.8%, 394.5%, and 549.6%,respectively, compared to the no mulch plus rainfed control. The maximum IWUE values for plant biomass [1.39 metric tonnes (MT) ha^–1 m^–1] and fruit yield (2.79 MT ha^–1 m^–1) were recorded under treatment M3I5 [i.e., black polyethylene mulch plus drip irrigation (0.6 V)]; whereas, the lowest values (0.39 and 0.68 MT ha^–1 m^–1, respectively) were observed using treatment M1I2 (i.e., without mulch, plus surface irrigation). Fruit size, weight, sugar content, and anthocyanin content increased significantly under treatment M2I3 [i.e., hay mulch, plus drip irrigation (1.0 V)] compared with all other treatments. Total soluble solids (TSS) contents and total acidity (TA) were highest under treatment M1I1 (i.e., no mulch, plus rainfed). A linear regression model could describe the variations in quality parameters of strawberry plants grown under sub-temperate climatic conditions. Root density was found to be the best indicator with which estimate fruit quality.     

高温条件下芥蓝菜薹色泽的形成

通过对高温条件下3个芥蓝品种菜薹色素及其相关因素研究,结果表明:芥蓝菜薹的叶绿素、花青苷和类黄酮含量尖叶夏芥蓝均为最高,荷塘芥蓝次之,中花芥蓝最低。尖叶夏的有机酸含量最高,中花次之,荷塘最低。花青苷含量较高的品种,其苯丙氨酸解氨酶(PAL)活性也比较高。芥蓝菜薹表皮色泽是叶绿素、类黄酮和花青苷等色素的综合表现,并受表皮酸碱性的影响。     

Physico-chemical and antioxidant properties of six apple cultivars (Malus domestica Borkh) grown in southern Brazil

The objective of this study was to compare the physico-chemical properties and antioxidant activity of six apple cultivars grown in southern Brazil. Apple peel color, dry matter, total soluble solids, pH, total sugars, titratable acidity, total phenolics, total monomeric anthocyanin and total antioxidant activity were measured in the apple cultivars Imperatriz, Daiane, Fred Hough, Fuji Suprema, Galaxy and Baronesa. The results showed great quantitative differences in the composition of the apple cultivars. Of all the cultivars, the peel of Galaxy was slightly more red-colored and that of Fred Hough was the least red-colored. The dry matter varied from 15.24% (Galaxy) to 19.55% (Fuji Suprema), the soluble solids content was between 11.8 (Fred Hough) and 14.0 (Daiane) °Brix, pH values varied from 3.90 (Imperatriz) to 4.27 (Fred Hough), the total sugar content (g 100 g⁻¹) ranged from 11.54 (Imperatriz) to 14.78 (Fuji Suprema) and the titratable acidity content (g 100 g⁻¹) varied from 0.20 (Baronesa) to 0.36 (Imperatriz). The total phenolic content (GAE 100 g⁻¹ fresh matter) observed in the apple cultivars was between 105.4 (Baronesa) and 269.7 mg (Imperatriz). The values of the total anthocyanin content (mg 100 g⁻¹ FM) ranged from 4.79 (Fred Hough) to 41.96 (Galaxy). The highest total antioxidant activity was observed in Imperatriz (739 μmol TEAC 100 g⁻¹ FM), while the lowest value was found in Fuji Suprema (335 μmol TEAC 100 g⁻¹ FM). There was a strong correlation between total monomeric anthocyanin content and all peel color measurements and between antioxidant activity and total phenolic content. The results suggested that genotype is the main factor that determines the composition of bioactive compounds in apples and this provides important information on how to make the best use of the apple cultivars investigated.     

葡萄杂交后代主要经济性状的遗传倾向

通过对10个杂交组合与5个自交后代801个株系的遗传变异分析结果表明:果粒重量、含糖量和含酸量的遗传均符合数量性状连续变异的遗传特点。子代果粒重量小于亲本值,并趋向小粒亲本,出现超高亲个体的比例较低,平均遗传传递力为70.34％。欧美杂交种品种间杂交和欧亚种与欧美杂交种间杂交传递力高于欧亚种品种间杂交;可溶性固形物含量均值接近亲本值,超高亲比例大,表明含糖量遗传中加性效应较大,在后代中选择高含糖量单株的潜力大,组合传递力为101.7％,欧美杂交种品种问杂交传递能力较弱,而欧亚种与欧美杂交种杂交,性状传递能力较强;含酸量的遗传中含有较大比例的负向非加性效应,子代含酸量大大高于亲本值,后代含酸量普遍升高,超低亲单株比例低,选择难度较大。平均组合传递力为134％,欧亚种品种问杂交遗传传递力高于欧美杂交种品种间和欧亚种与欧美杂交种种间杂交。     

葡萄新品种‘新郁’

 ‘新郁’是以‘红地球’自然杂交单株E42-6为母本, ‘里扎马特’为父本杂交选育而成, 在新疆鄯善地区9月上中旬果实成熟, 果粒椭圆形, 果皮紫红色, 平均单粒质量11.6 g, 果肉较脆, 味酸甜,可溶性固形物含量16%～19% , 总酸为0.33%～0.39% , 贮运性能较好, 栽培适应性较强。     

根域加温对促成栽培绯红葡萄生长发育的影响

连续2年以“Y”形整枝的2年生葡萄绯红(VitisviniferaL.cv.Cardinal)品种为试材,在超早促成栽培的早期进行根域土壤加温处理,探讨其对根域土壤温度、树体发育和果实品质的影响。结果表明,根域加温提高促成栽培早期土壤温度约10℃,各物候期均早于未加温处理2～5d,新梢生长好,叶面积大;花穗发育好,单株果穗数显著增加;果汁含糖量和果皮花青素含量增加,可滴定酸降低,果实品质提高;果粒重量、果穗重量和单株产量均显著高于未加温处理。并就根域加温效果的形成机理进行了探讨。     

油菜素内酯和脱落酸调控葡萄果实花色苷合成的研究

【目的】为研究2,4-表油菜素内酯(2,4-epibrassinolide,EBR)和脱落酸(abscisic acid,ABA)处理对葡萄花色苷合成与可溶性固形物含量、果皮苯丙氨酸解氨酶(PAL)和类黄酮糖基转移酶(UFGT)活性以及果实内源ABA含量的影响,探索油菜素内酯调控葡萄果实成熟及花色苷合成的机理。【方法】以酿酒葡萄‘赤霞珠’(Cabernet Sauvignon)和‘烟73’(Yan 73)为试材,在葡萄转色前分别用0.1、0.4、0.8 mg.L-1EBR,1mg.L-1Brz(brassinazole,BR生物合成抑制剂)和200 mg.L-1ABA,均匀喷施于葡萄果实,在葡萄成熟过程中测定葡萄果皮花色苷含量及PAL和UFGT酶活性,同时测定果实ABA和可溶性固形物含量。【结果】在果实着色初期,‘赤霞珠’和‘烟73’葡萄果皮PAL和UFGT活性及果实内源ABA含量均逐渐升高,当果实接近成熟花色苷含量基本稳定时ABA含量开始降低。与对照相比,0.4mg.L-1EBR和200 mg.L-1ABA处理显著增加了果实内源ABA含量,提高了果皮PAL和UFGT活性,促进了果皮花色苷的合成和果实可溶性固形物的积累。0.1 mg.L-1和0.8 mg.L-1EBR处理总体增加了果实内源ABA含量,促进了花色苷的合成和可溶性固形物的积累,并提高了UFGT和PAL酶活性,但比0.4 mg.L-1EBR处理提高的幅度小,且差异显著。1 mg.L-1Brz处理使果实ABA的合成推迟,果实可溶性固形物含量、果皮PAL和UFGT活性以及花色苷含量均低于对照,但差异不显著。【结论】外源EBR和ABA处理促进了葡萄成熟和花色苷合成,在不同浓度EBR处理中,以0.4 mg.L-1处理效果较好;内源ABA可能参与了EBR对葡萄成熟和花色苷合成的调控。     

甜樱桃和酸樱桃品种果实性状的综合评价

以‘早大果’、‘红灯’等14个生产上早栽培甜樱桃品种和1个酸樱桃品种‘CAB'为试材,研究了不同品种间形态性状、内在品质和主要内含物含量的差异显著性,并对各项测定指标进行相关性检验.结果表明:15个樱桃品种以单果均重、总酸、还原糖、可溶性固形物、花色苷、总酚和维生素C含量差异最明显,可见樱桃果实大小、风味和内含物含量受品种影响显著.相关性检验显示,总酚与总酸;花色苷与还原糖、可溶性固形物;果形指数与总酸、pH值;单果均重与单核均重;果实汁液色泽与糖酸、可溶性固形物、花色苷和总酚呈显著相关关系.     

葡萄园生草对葡萄与葡萄酒质量的影响

大多数研究认为,葡萄园生草可有效控制植株的生长势,减少夏季和冬季修剪量,促进根系向土壤深层发展,生草能降低葡萄叶片营养元素含量和葡萄果实产量,可调节葡萄园温度和湿度,改善葡萄园微气候,减少病虫害,提高果实品质,使果实含酸量降低,含糖量和糖酸比升高,同时提高了红葡萄酒中花色素苷、多酚含量及pH,使酒体颜色加深,结构感增强,从而提高葡萄酒的质量。今后应进一步加强葡萄园生草对葡萄与葡萄酒质量影响机理的研究。     

葡萄新品种——申华的选育

申华是以京亚为母本,优系86-179为父本,通过有性杂交育成的优质葡萄新品种。该品种经无核化栽培后,平均穗质量463 g,平均粒质量13 g,果皮紫红色,可溶性固形物16%-17.5%,无核率达100%,风味浓郁,上海地区设施促成栽培7月中旬果实成熟。     

Effect of bearing position on phenolics profiles in the skins of four cultivars of grapevine (Vitis vinifera L.)

Summary

The fan-shaped trellis training system, resulting in grapevines (Vitis vinifera L.) with more than two trunks, has been widely used in northern China. The fruiting zone of grapevines trained to the fan-shaped trellis system is distributed from the top to the bottom of the canopy. The phenolics profiles of the skins of ‘Cabernet Sauvignon’, ‘Merlot’, ‘Chardonnay’, and ‘Italian Riesling’ grape berries were analysed to measure the effect of differences in bearing position on the phenolic compound and anthocyanin compositions of grape berries.The results showed that the upper bearing position significantly increased the concentrations of most phenolic compounds in grape berry skins. Among the non-anthocyanin and anthocyanin phenolic compounds identified in this study, the upper bearing position promoted the accumulation of delphinidin-, cyanidin-, and petunidin-glucosides in the skin of the two red grape cultivars. However, lowering the bearing position was associated with greater methylation of anthocyanins, and the flow of photosynthate towards the biosynthesis of kaempferol. These results were correlated with micrometeorological alterations in the canopy at the different bearing positions. The findings of this study suggest that higher bearing positions can increase the accumulation of phenolic compounds in grape berry skins in four different grapevine cultivars.     

Correlation of metabolites in the leaf and berries of selected black pepper varieties

The biochemical component which attribute pungency to black pepper (Piper nigrum) is mainly the alkaloid piperine, the aroma and flavour are attributed by components like α- and β-pinenes, sabinene, myrcene, limonene, β-caryophyllene, camphene, etc. Our study revealed that the biochemical profile varies in the leaf and berries of black pepper. Total phenols, total starch, total carbohydrate and protein content from leaves and berries of selected 26 black pepper cultivars were evaluated. The concentration of oil, oleoresin, piperine and the essential oil constituents from both leaves and berries were also compared in these cultivars. Germacrene-D and elemol were found to be the major constituents of leaf oil. β-Caryophyllene was high in berries and it showed more variability in berries compared to leaf samples. Berry oil constituents namely, pinene, sabinene, myrcene and limonene were not detected in the leaf oil. Different leaf metabolites showed cumulative direct effect on berry constituents.     

Dietary Elements and Quality Parameters of 34 Old and Eight Commercial Apple Cultivars Grown at the same Site in South Tyrol,Italy

Apple is one of the most widely produced and consumed fruits worldwide and hence, complete data of apple composition are important for human diet. Currently, a limited number of cultivars dominate the market, while many others, with a potentially higher nutritional value, are neglected by consumers. The present work reports the content of the dietary elements potassium (K), phosphorus (P), calcium (Ca) and magnesium (Mg) as well as the content of the macroelement nitrogen (N) of 34 old cultivars grown at the same site under identical conditions in South Tyrol, Italy. Their elemental composition was assessed along with quality parameters such as fruit weight, firmness, and soluble solid content and total acidity at harvest and post storage. For selected cultivars the measurements were performed over two or even three different harvest years. Comparison with eight commercial cultivars chosen to represent the fruit currently dominating the market was performed.Besides offering a valuable insight in the variation of dietary elements among old and commercial apple cultivars in up to three harvest years, this study, that complements current nutritional databases, recommends several old cultivars with high content of dietary elements for further study and eventual re-introduction in niche markets.