Carbohydrate changes in kiwifruit buds during the onset and release from dormancy

Carbohydrates were measured in axillary bud meristems from kiwifruit (Actinidia deliciosa (A.Chev.) C.F. Liang et A.R. Ferguson cultivar Hayward) to determine whether concentrations changed during winter dormancy. Buds were collected from kiwifruit vines growing in four sites, which spanned the climatic range that kiwifruit are grown in New Zealand, and from vines where the time of budbreak had been manipulated using hydrogen cyanamide. During winter, sugars comprised 78% of measured carbohydrates in meristems, with sucrose accounting for more than 86% of sugars detected (up to 200 mg g DW⁻¹). Starch concentrations in bud meristems showed little change during winter. Meristem sucrose and hexose concentrations increased rapidly from autumn until mid-winter, and this was correlated with leaf abscission. Sugar accumulation ceased in mid-winter and concentrations remained stable until spring, suggesting that bud meristem activity was low. Four weeks before visible bud movement, a marked decline in sucrose and increase in hexose concentrations, indicated activity in the meristems had increased prior to budbreak. The patterns of seasonal change and peak sucrose concentrations in meristems were similar at all sites. The only difference was in the timing of events, with changes in meristem sugars occurring earliest in vines growing at the coolest site, coinciding with their earlier cessation of and resumption of vine growth. Advancing budbreak, by applying hydrogen cyanamide to vines in late winter, also advanced changes in sugar concentrations within meristems. This work suggests that the concentration of sucrose in kiwifruit bud meristems has utility as an indicator of whether they are being induced into, maintained or released from winter dormancy.     

Temperature effects on dormancy,bud break and spear growth in asparagus (Asparagus officinalis L.)

Summary

The effects of the length of chilling, chilling temperature and growing temperature on dormancy of asparagus crown buds and subsequent rates of spear growth were examined. The results showed that prior chilling enhanced bud break at low growing temperatures and stimulated the growth of spears.Thus, chilling should facilitate commercial production by hastening bud break and spear growth rates at lower temperatures. If sufficient chilling was given, the minimum temperature for rapid bud break was approx. 12.5°C for ‘Rutgers Beacon’ and ‘Jersey Giant’, and around 10°C for ‘UC 157’ and ‘Apollo’. The optimum chilling temperature appeared to be closer to 5°C than to 10°C or 2°C for ‘Rutgers Beacon’ plants grown at 12.5°C. Increasing the growing temperature had a significant effect on the relative spear growth rate (RSGR) in all cultivars. Prior chilling had no effect on the RSGR for ‘Dariana’ and ‘Apollo’; but, for ‘UC 157’, chilling plants at 5°C for 5 or 10 weeks increased growth rates at 12.5°C and at 20°C. These results demonstrate that release of bud dormancy and spear growth rates depended not only on the growing temperature, but also, at least in some cultivars at some temperatures, on the duration and temperature of chilling during the previous Winter.     

Response of the chrysanthemum to soil heating

Chrysanthemum plants were exposed to 16°C day-temperature, 11°C night-temperature, 13°C soil-temperature, to be indicated as 16/11/13°C, or to 16/11/25°C, 20/16/18°C, or 20/16/25°C, first long day, then short day, (long day = 12-h light period with 3-h night break; short day = 12-h light period) from planting to harvest in controlled environments to study the effects of soil heating on growth and flowering. There were significant, but not substantial, effects of soil heating on leaf area, percent soluble carbohydrate, flower bud diameter, days to visible bud and some other parameters. Two winter cultivars responded similarly, while 2 summer cultivars differed in flowering-response to soil heating. An experiment was also conducted using 16/11/25°C day/night/soil temperatures during long days, short days or throughout the complete growth cycle, with 16/11/13°C day/night/soil temperatures at other times. Soil heating during long days resulted in the highest quality flowers. Soil heating during short days or throughout the growth period resulted in most rapid flowering but decreased flower quality.     

Bud dormancy of apple and pear in relation to the temperature during the growth period

In 2 experiments, apple and pear trees were grown in growth chambers and air conditioned greenhouses at a range of constant temperatures from 9 to 25° C during spring and summer. After the dormant buds had received a marginal chilling-period, bud break and leaf development were studied. A tendency was observed for the dormancy to be deeper when the temperature at which the buds were formed had been higher. The maximum retardation of bud break was observed in buds formed at 18 and 21° C. The hypothesis is put forward that the phenomenon of “delayed foliation” is not only due to insufficient chilling during winter but also to high temperatures during the preceding summer.     

Flowering of cacao under controlled temperature conditions

The relationship between flowering and day and night temperatures in cacao has been studied over a period of nine months in controlled environment rooms, with clonal trees which were 13 months old at the start of the experiment.

All the plants started to flower at the same time, but thereafter there was a marked response to temperature. Flowering was greater at day temperatures of 80° and 86° F. (26 .7°, 30° C.) than at day temperatures of 74° F. (23–3° C.) and, at each level of day temperature, flowering was greater at a night temperature of 80° F. than at one of 74° or 86° F. The relative effects of temperature were similar on numbers of flowering cushions per plant and of flowers per cushion.

There was no apparent relationship bfetween the amount of flowering and new leaves (flushes) produced, either at the time of flowering or at any period before. Neither was there a quantitative relationship between flowering and leaf area of the plants, though, in general, the treatments that resulted in the greatest leaf areas also resulted in the greatest numbers of flowers. A possible relationship was suggested between the number of flowering cushions and the total extension growth of the branches.     

Propagation temperature,PPFD, auxin treatment,cutting size and cutting position affect root formation,axillary bud growth and shoot development in miniature rose (Rosa hybrida L.) plants and alter homogeneity

Summary

Rooting and growth responses of miniature rose cuttings were investigated in an experiment in which four propagation temperatures, two photosynthetic photon flux densities (PPFDs) with five auxin (IBA) concentrations, cutting sizes and cutting positions, were combined factorially in a response-surface design. Most prominently, temperature, cutting size and auxin and their interactions, influenced root and shoot growth. A propagation temperature of 24.6°C, and IBA concentrations between 10^–3 and 10^–1M, depending on temperature, were optimal for root formation. Root formation in extra short cuttings was delayed at low IBA concentrations. Regarding root formation, IBA could substitute for increased temperature as well as for increased cutting size. Onset of axillary bud growth was fastest at 24.6°C, and delayed in extra short cuttings. Application of IBA at 10^–4 to 10^–3M was optimal for axillary bud growth. By increasing the IBA concentration both time to flowering and plant height increased at 24.6°C. In cuttings from higher positions on stock plants, axillary shoots enhanced their growth to flowering, became shorter, and weighed less, suggesting occurrence of positional effects (topophysis). The growth rate increased with increasing IBA concentration, as well as from medial to low positioned cuttings. Increasing propagation PPFD from 46 to 72 µmol m^–2s^–1 did not affect the parameters. Time to axillary bud growth and time to first flower were related to time-to-visible root. Fast formation of roots apparently resulted in fast axillary bud growth. In time-to-visible root and axillary bud growth, the smallest variation between plants was found at optimal ranges for temperature, IBA concentration and cutting size, and further factors optimal for root formation and axillary bud growth provided the most synchronized plant development.     

Bitter Rot of Apples: II. Seasonal Variations in The Development and Sporulation of Cankers of Gloeosporium SPP. Inoculated into Apple Branches

Two-year-old potted trees of Cox’s Orange Pippin, Laxton’s Superb and Worcester Pearmain were grown in controlled environment chambers at Long Ashton during 1960 and 1961, and subjected to four types of winter temperatures : 1, a long, cold winter ; 2, a severe autumn and mild spring ; 3, a mild autumn and severe spring ; 4, a short, mild winter ; 5, natural winter.

The order of bud break was treatment 2 followed by 4, then I and lastly 3. The time of bud break was more strongly influenced by the date of winter chilling than by its intensity, but the intensity influenced the temperature at which bud break became possible. On I-year-old wood the number of leafy rosettes developing increased with the length of the chilling period.

Because of limitation of space and the consequent small number of trees used, no firm conclusions could be drawn, but flower bud production and rate of spur development from bud break to full bloom appeared to be favoured by a mild autumn. An early and severe autumn apparently increased flower bud abscission at green cluster, but no effect of treatments could be determined in the final yields of fruit.     

Flower Bud Cold Hardiness of ‘Muskoka’ Red Raspberry as Related to Water Content in Late Winter

Abstract

‘Muskoka’ red raspberry (Rubus idaeus L.) canes were sampled from a field (latitude 60°10′N) at 2- to 3-week intervals from February to April to examine whether the loss of cold hardening capacity in late winter is connected to the increase in bud water content, or to the increased ability of buds to absorb water. Bud base vascular tissue lost its hardening capacity earlier than floral primordia and was injured at warmer temperatures. Cold hardiness was more closely correlated with bud water content than with water potential. Both low water content and low water potential indicated the ability of tissue to harden at low temperatures. All determined parameters except hardening capacity were positively correlated with mean air temperature during a 2-day period preceding sampling. Loss of bud base vascular tissue hardening capacity coincided with increases in bud water content, maximal water content and water potential in the beginning of April, after diurnal mean temperature had risen above 0°C.     

Promotion of floral initiation in ‘Fuerte’ avocado by low temperature and short daylength

Potted avocado (Persea americana Mill., cv. ‘Fuerte’) plants were maintained in growth cabinets for up to 32 weeks and new growth observed for flower formation. Flowers were formed if temperatures were 20°C or below, but with 25° or 30°, even if only for 1 hour per day, flower formation was inhibited. Time to flowering was accelerated, but number of flowers reduced, if daylength was shortened from 15 h to 9 h. With low temperature and short days, full bloom was about 4 months after starting experiments. Spring flowering of cv. ‘Fuerte’ in the field could follow flower induction about 4 months previously with the onset of winter temperatures and daylengths.     

Long-day control of flowering in everbearing strawberries

Summary

Photoperiod and temperature control of flowering in a number of perpetual-flowering or everbearing strawberry cultivars of widely varying pedigree has been studied in controlled environments. Flower bud initiation in the cultivars ‘Flamenco’, ‘Ridder’, ‘Rita’ and ‘Rondo’ was significantly advanced by long-day (LD) conditions at temperatures of 15°C and 21ºC; while, at 27ºC, flowering took place under LD conditions only. Some plants of the seed-propagated F₁-hybrid ‘Elan’, raised at 21°C, also flowered under short-day (SD) conditions at 27°C, but reverted to the vegetative state after a few weeks when maintained under these conditions. When vegetative plants growing in SD at 27°C were transferred to LD conditions at the same temperature, they consistently initiated flower buds and started flowering after about 4 weeks. At such a high temperature, flowering could thus be turned on and off by switching between SD and LD conditions. This applied to all the cultivars studied. Also the cultivar ‘Everest’, which was tested only at 21°C, produced similar results. Night interruption for 2 h was effective in bringing about the LD response. At 9°C, flowering was substantially delayed, especially in ‘Flamenco’ and, at this temperature, flowering was unaffected by photoperiod. Runner formation was generally promoted by high temperature and SD conditions, but the photoperiodic effect varied between experiments. We conclude that everbearing strawberry cultivars, in general, whether of the older European-type or the modern Californian-type originating from crosses with selections of Fragaria virginiana ssp. glauca, are qualitative (obligatory) LD plants at high temperature (27°C), and quantitative LD plants at intermediate temperatures. Only at temperatures below 10°C are these cultivars day-neutral.     

The effect of cane orientation on flower production in kiwifruit (Actinidia deliciosa)

Summary

The effects of training replacement canes of kiwifruit either downwards (45° below horizontal) on a T-bar support, or upwards on a 60° sloping trellis were studied during one season. Training canes upwards reduced the percentage bud burst by 19% and the number of flowers per cane by 20%. There was no significant effect on the growth rate of the apical shoot, or on the number of flowers per flowering shoot. The timing of bud burst and flowering were not affected by cane orientation. The implications of these results for vine management techniques are discussed.     

Environmental interaction between day length,night temperature,and photon flux density on growth and flowering in Boronia megastigma Nees.

Summary

Boronia plants were placed in a range of environmental conditions, two night temperatures (6 and 15°C), three photon flux densities (full sunlight, 50% and 30%), and two day lengths (natural autumn to winter cycle and natural autumn to winter cycle plus synthetic to 16 h total light). Flowering occurred to some extent under all conditions; generally conditions suitable for high growth rates were not optimum for flowering, maximum numbers of flowers were achieved with low night temperatures (6°C), short days (10 h), and full to 50% sunlight. Floral development was influenced by all treatments.     

Environmental and cultural effects on vegetative growth and flowering of selected “bedding” ornamentals. I. Night temperature

Night temperatures warmer than those normally used in commercial production systems promoted vegetative growth in Tagetes patula and Matthiola incana seedlings. In the short (8 h) daylength regime employed, and with a day temperature of 16°C, cool nights (8°C) had a detrimental effect, especially when imposed for 6 weeks from pricking-out rather than later. Night temperatures between 8°C and 16°C did not substitute for the long-day requirement for flowering in Matthiola after 11 weeks' growth, and there was no advantage with respect to vegetative growth in maintaining night temperatures above 12°C. Warmer nights from pricking-out promoted flowering in Tagetes but if applied later (during weeks 7–11) the positive effects were largely on vegetative growth. The implications of these findings for commercial horticulture are discussed.     

Chilling induced ethylene biosynthesis in ‘Hayward’ kiwifruit following storage

‘Hayward’ kiwifruit were stored at 0, 5, 10, 15 and 20°C for 5, 12 and 17 days before rewarming to 20°C for 10 more days. Ethylene and CO₂ production, ACC, ACC synthase (ACS) and ACC oxidase (ACO) activities, flesh and core firmness, soluble solids content (SSC) and flesh colour were measured. Kiwifruit stored at 0, 5, 10 and 15°C did not ripen, produce ethylene or show increases in ACS or ACO activity. Fruit stored for 5 days at the above temperatures, then rewarmed to 20°C, did not show any change during the following 10 days. Rewarmed fruit, pre-stored at 0–10°C for 12 days, started autocatalytic ethylene production within 24 h, followed by fruit ripening. Fruit stored at 15°C for 12 days needed 72 h to start ethylene autocatalyse and did not fully ripen during 10 days at 20°C. After 17 days storage at 0–15°C kiwifruit started autocatalytic ethylene production with no delay upon exposure to 20°C. Autocatalytic ethylene production correlated with increased ACC content, and increased activities of ACS and ACO. Fruit held continuously at 20°C started autocatalytic ethylene production after 19 days, with concomitant increases in ACC content, ACS and ACO activities and ripening. Respiration increased after rewarming, concomitantly with the increase in ethylene production.We concluded that exposing kiwifruit to chilling temperatures (0–10°C) for 12 days advanced ethylene biosynthesis and ripening when compared with fruit held continuously at 20°C. The advanced ethylene biosynthesis was due to increase ACS and ACO activities immediately upon rewarming of the fruit.     

Flowering and Fruiting in ‘Arbequina’ Olives in Subtropical Climates Where Olives Normally Remain Vegetative

Abstract

It is generally assumed that lack of flowering and fruiting in olives in subtropical climates of southern Texas (e.g. Weslaco Texas area; N 26.16° Latitude 97.96° Longitude) is due to fewer chilling days (<7.2°C) during winter than most olive growing areas of the world. However using controlled environmental chambers we have recently shown that flowering and fruiting in the ‘Arbequina’ cultivar of olives can be achieved without any chilling days. This raised the question of why olive trees don't flower in southern Texas where they do experience some chilling days. We hypothesize that the absence of flowering in olives growing in southern Texas and a similar climate elsewhere is due not to the lack of enough chilling days but most likely due to high temperatures during the day (≥°C) that inhibit flowering. To test our hypothesis we provided cooling to olive trees growing under Weslaco Texas climate by simple shading or by evaporative cooling. These treatments resulted in good flowering and fruiting in ‘Arbequina’ olives in Weslaco Texas after a typical winter period although normally olive trees in this subtropical climate remain vegetative even after winter months.     

The effects of day and night temperature on Chrysanthemum morifolium: investigating the safe limits for temperature integration

Summary

The impact of day and night temperatures on pot chrysanthemum (cultivars ‘Covington’ and ‘Irvine’) was assessed by exposing cuttings, stuck in weeks 39, 44, and 49, to different temperature regimes in short-days. Glasshouse heating set-points of 12°, 15°, 18°, and 21°C, were used during the day, with venting at 2°C above these set-points. Night temperatures were then automatically manipulated to ensure that all of the treatments achieved similar mean diurnal temperatures. Plants were grown according to commercial practice and the experiment was repeated over 2 years. Increasing the day temperature from approx. 19°C to 21°C, and compensating by reducing the night temperature, did not have a significant impact on flowering time, although plant height was increased. This suggests that a temperature integration strategy which involves higher vent temperatures, and exploiting solar gain to give higher than normal day temperatures, should have minimal impact on crop scheduling. However, lowering the day-time temperature to approx. 16°C, and compensating with a warmer night, delayed flowering by up to 2 weeks. Therefore, a strategy whereby, in Winter, more heat is added at night under a thermally-efficient blackout screen may result in flowering delays. Transfers between the temperature regimes showed that the flowering delays were proportional to the amount of time spent in a low day-time temperature regime. Plants flowered at the same time, irrespective of whether they were transferred on a 1-, 2-, or 4-week cycle.     

Growth responses of some greenhouse plants to environment. II. The effect of soil temperature on Chrysanthemum morifolium Ramat

The effect of a wide range of soil temperatures (6–26°C) on growth and flowering of Chrysanthemum morifolium Ram. ‘Horim’ were studied at the favourable air temperature of 18°C. Shoot growth was severely reduced at soil temperatures below 10°C which may be explained by poor root growth, while flowering was enhanced by approximately 2 days compared to higher soil temperatures. Increasing the soil temperature to 18°C was beneficial. Further increase had no positive effect on growth. Measurements of net photosynthetic rates revealed no effect of lowering soil temperatures from 18 to 6°C.Mother plants grown at 18°C air temperature revealed no effect of soil temperatures ranging from 13 to 21°C on number and fresh weight of the cuttings. Neither did mother plants grown at the less favourable air temperatures of 12 or 15°C. Cutting production was, however, affected by air temperature.     

Einfluss rezenter Klimaveränderungen auf die Phänologie bei Kernobst am Standort Klein-Altendorf – anhand 50-jähriger Aufzeichnungen

This project examines the effects of climate change on pome fruit phenology at Klein-Altendorf in the Rhineland fruit-growing region in the West of Germany, using 50 years of weather data and phenology records, including beginning of flowering (F1), full bloom (F2), harvest date and leaf drop of apple and pear, with the following results:

1. Fifty years of weather records and pome phenology data showed a distinct separation between a first phase (1958–1987), which was 0.42°C cooler, and a second phase (1988–2007), which was 0.66°C warmer than the long-term, 50 year temperature average of 9.4°C, indicating the start of the climate change in 1988.
2. Annual average temperatures rose from 8.8°C to 10.2°C, i.e. by 1.4°C over 50 years at Klein-Altendorf, which affected the apple phenology. A comparison of the second phase (average temp. 10.1°C) with the first phase (9.0°C) resulted in a temperature rise of 1.1°C; the temperature rise in the winter (1.2°C) was greater (by 0.2°C) than in the summer (1.0°C). This temperature rise in the last 20 years (second phase, 1988–2007) was more pronounced in the winter months January ( $ + $ 1.7°C) and February ( $ + $ 1.8°C) than in the spring months March and May both with $ + $ 1.4°C leading to enhanced early post-bloom fruit drop, and July ( $ + $ 1.3°C) and August ( $ + $ 1.6°C).
3. Annual precipitation remained constant at ca. 600?mm with an increase in February, March and April and an equivalent decrease in May (?10?mm) and July (?7?mm) during fruit development.
4. Soil temperatures increased by 2.6°C and hence greater than the air temperature ( $ + $ 1.45°C) over the last 50 years; this increase was most pronounced between January and March.
5. Minimum air temperatures increased also by 2.6°C from ?6.1°C in March 1958 to ?3.5°C in March 2007. The more advanced flowering, brought about by warmer January to March temperatures, now coincides with late spring frosts, thereby maintaining the risk of yield loss due to spring frost in April, a month when the minimum temperature still continues to drop.
6. On average, apple and pear flowering was 10 days earlier without extension of the flowering period after warm winters and appeared more severely affected than the harvest period (3–9 days) and leaf drop (2–3 days earlier) with distinct varietal differences. The apple flowering period was 4 days shorter in the last 10 years and reduced from 12–15 days to 8–10 days. Early maturing cultivars (cvs) ripened 3–4 days earlier, while late cvs ripened 8–12 days earlier. The period between harvest and leaf drop was extended by 2–7 (apple) to 11 days (pear), which requires regional differentiation according to the respective climatic zone and may be beneficial for nutrient translocation into the perennial woody parts of the tree.
7. Since the changes in the temperature-based vegetation period did not explain the effects of climate change on pome phenology, a `pomological vegetation interval' between the beginning of flowering and beginning of leaf drop was defined and was extended by climate change in spring by 0–10 days in apple and by 8 days in pear. Based on flowering (F1, F2) advances, the Meckenheim fruit-growing region was more strongly affected by the climate change compared with other European fruit growing regions.

     

Increasing Flower Number in Single-Truss Tomatoes

Flower number in the first truss (inflorescence) of glasshouse tomatoes was increased by growing the seedlings at low temperatures shortly after pricking off. The optimal timing for this treatment varied by only a few days over most of the year. The longest chilling duration (14 days) was most effective, possibly because of variability between plants. In summer flower number could be doubled, but in winter only 30% to 40% increases were obtained. Chilling delayed anthesis by up to ten days, the delay being proportional to the duration of chilling.

Day/night temperatures of either 10°/10° C. or 16°/4° C. during chilling had similar effects on flowering, and resulted in similar delays to growth and anthesis.

The mechanism by which chilling may increase flower number and the distinctions between this process and vernalization are discussed.