遮光对黄瓜幼苗同化物代谢相关糖和酶的影响

试验中发现,黄瓜幼苗遮光叶片蔗糖含量略高于未遮光对照叶片,而棉籽糖、肌醇半乳糖苷和水苏糖含量显著低于对照叶片。叶片中肌醇半乳糖苷合成酶活性和基因转录水平变化趋势一致,遮光叶片显著低于对照叶片;棉籽糖合成酶活性差异不显著,但是遮光叶片棉籽糖合成酶基因转录水平显著低于对照叶片;遮光叶片水苏糖合成酶活性显著低于对照,且相关基因转录水平变化与酶活性变化一致;此外蔗糖非发酵相关蛋白激酶1基因（SnRK1）表达水平在遮荫处理6 h时显著高于对照。表明在遮光胁迫下,黄瓜幼苗通过调控肌醇半乳糖苷合成酶、水苏糖合成酶和蔗糖非发酵相关蛋白激酶1相关基因表达和酶活性,维持叶片蔗糖含量,提供生长所需能量,保证叶片存活。     

Changes in carbohydrate levels and their metabolic enzymes in leaves,phloem sap and mesocarp during cucumber (Cucumis sativus L.) fruit development

Levels of carbohydrates and activities of metabolic enzymes were examined in leaves (source), phloem sap (flow) and mesocarp tissues (sink) in the course of cucumber (Cucumis sativus L.) fruit development, from 2 days before anthesis to 20 days after anthesis. While total sugar levels increased in all the three sampling organs, starch levels declined in leaves and mesocarp tissues as fruit development progressed. Glucose and fructose were the primary contributors to the soluble sugar pools in mature leaves. Stachyose was found as the most important component of the phloem sap extracts, followed by sucrose and raffinose. However, the primary sugars accumulated in mesocarp tissues were glucose and fructose, not stachyose or sucrose. Activities of sucrose synthesizing enzymes (sucrose phosphate synthase plus sucrose synthase in the synthesizing direction) exceeded that of sucrose degrading enzymes (acid invertase, neutral invertase plus sucrose synthase in the degrading direction) in leaves, which might cause a sucrose pool utilized in raffinose and stachyose biosynthesis. While alkaline a-galactosidase form I activity declined, stachyose synthase activity showed a rapid increase until 12 days after anthesis and only subsequently decreased in leaves. Activities of sucrose degrading enzymes were always much higher than that of sucrose synthesizing enzymes in mesocarp tissues. Thus, sucrose accumulation could not occur in mesocarp tissues. While stachyose synthase activity steadily decreased, alkaline a-galactosidase form I activity showed a moderate increase before decrease in mesocarp tissues. The relationship between levels of soluble sugars and activities of relative enzymes was also discussed.     

短期夜间亚低温及恢复对番茄光合作用和蔗糖代谢的影响

 为了明确番茄叶片光合作用及蔗糖代谢对夜间亚低温的响应机制,系统研究了9 ℃夜间亚低温及其恢复对番茄净光合速率、各器官干物质量、碳水化合物积累、蔗糖代谢酶活性的影响。结果表明：9 ℃夜间亚低温降低了番茄净光合速率和各器官的干物质量,增加了淀粉、果糖、葡萄糖、蔗糖和总糖的含量;抑制了番茄中酸性转化酶（AI）、中性转化酶（NI）、蔗糖合成酶（SS）和蔗糖磷酸合成酶（SPS）活性;夜间9 ℃亚低温处理6 d内,在15 ℃适温恢复9 d时番茄光合速率、各器官干物质量、淀粉、果糖、葡萄糖含量等均可恢复到对照水平,但处理9 d时再恢复9 d,光合速率、淀粉和果糖含量、SS活性均未能恢复到对照水平,特别是处理6 ~ 9 d时再恢复9 d,蔗糖、蔗糖合成酶（SS）和蔗糖磷酸合成酶（SPS）活性均未恢复到对照水平,且亚低温处理时间越长恢复所需时间越长。从蔗糖和淀粉含量分别与净光合速率和SPS活性呈极显著负相关关系的结果看,夜间亚低温导致的净光合速率的降低可用“光合末端产物的反馈抑制”来解释,说明源叶中光合末端产物的积累可能是光合效率降低的直接原因之一。     

高昼温对日光温室番茄叶片碳水化合物代谢的影响

以‘辽园多丽’番茄为试材,以昼温25℃为对照,对植株进行30℃和35℃高昼温处理,探讨在高昼温条件下,叶片碳水化合物含量、蔗糖代谢酶活性以及叶绿体超微结构的变化。结果表明：高昼温尤其是35℃昼温处理,番茄叶片叶绿体超微结构发生改变,短时间内叶绿体中淀粉粒数减少,持续15 d后则明显增多,与对照差异显著。高昼温处理的番茄叶片中果糖、葡萄糖和蔗糖含量均降低,昼间温度越高,各糖分含量与对照间差别越明显。在高昼温处理15 d时,叶片中酸性转化酶(AI)、中性转化酶活性(NI)降低,而蔗糖合成酶(SS)、蔗糖磷酸合成酶(SPS)活性升高,昼温越高,蔗糖代谢相关酶活性与对照的差别越大。高昼温改变了番茄叶片叶绿体的超微结构,并且对糖分的积累与代谢产生影响,最终对植株的生长发育及产量与品质的形成不利,昼温越高,所造成的不利影响越大。     

越瓜果实发育过程中糖的变化与相关酶活性的关系

以从各地搜集的6 个越瓜品种为试材,对越瓜果实糖含量及蔗糖代谢相关酶进行了研究。结果表明：不同越瓜品种之间糖含量差异较大,凤台越瓜、田集越瓜、刘集越瓜3 个品种可溶性固形物含量及糖含量较高;随着果实的发育蔗糖磷酸合成酶活性呈递增趋势,且在果实各个发育期田集越瓜的蔗糖磷酸合成酶活性均最高;而转化酶活性则随着果实发育呈下降趋势,凤台越瓜和田集越瓜的酸性转化酶及中性转化酶活性在各个发育期均最低;中性转化酶在毛集越瓜、潘集越瓜、青皮越瓜、刘集越瓜中相对较高,且随着果实发育活性下降,至果实成熟时活性达到最小值;除刘集越瓜外,其他品种的蔗糖代谢酶活性在果实不同发育期均没有明显变化。以上结果表明,不同越瓜品种蔗糖含量差异是由蔗糖磷酸合成酶、酸性转化酶和中性转化酶活性共同决定的,其中酸性转化酶在越瓜蔗糖积累中占主导地位。     

短期夜间低温对栽培番茄和野生番茄果实蔗糖代谢的影响

 在人工气候室内以低温敏感的栽培番茄（Solanum lycopersicon）‘Money Maker’和耐低温的野生番茄（S. habrochaites）‘LA1777’为试材,研究了短期（4 d）夜间15 ℃处理和9 ℃、6 ℃及其恢复处理对番茄果实糖含量和蔗糖代谢相关酶活性的变化。结果表明：6 ℃处理0.5 d,耐低温的番茄（‘LA1777’）果实中果糖和葡萄糖含量升高,低温敏感番茄（‘Money Maker’）果实中两种糖含量无显著变化。6 ℃处理‘Money Maker’中蔗糖和淀粉的含量显著降低,而‘LA1777’中含量提高。在恢复期,两种番茄的蔗糖含量均恢复到对照水平,6 ℃处理的葡萄糖和果糖未恢复到对照水平,而9 ℃与对照无显著差异。6 ℃处理降低两种番茄果实中转化酶和蔗糖合成酶（SS分解方向）的活性,无法恢复至对照水平,‘Money Maker’果实中SS合成方向的活性和蔗糖磷酸合成酶（SPS）活性提高,而‘LA1777’变化不显著。说明短期夜间低温条件下耐低温的番茄是通过提高果实中总的碳水化合物含量来适应低温胁迫的,而低温敏感的番茄则是降低果实中碳水化合物含量来响应低温。     

Salicylic acid regulates sugar metabolism that confers tolerance to salinity stress in cucumber seedlings

Salicylic acid (SA) plays a critical role in plant development and defense responses to biotic and abiotic stresses. Sugars can act as osmotic adjustors or nutrient and metabolic signals in the activation of plant defense responses. To uncover the effects of SA on sugar metabolism, we assayed the altered components and levels of sugars in cucumber seedlings treated with SA. After SA treatment, the levels of glucose, fructose, raffinose and stachyose were increased in both leaves and roots. In contrast, sucrose and starch responded differently in leaves and root, decreasing in leaves but accumulating in roots. These changes could be due to the effects of SA on the activities of metabolism-related enzymes. In leaves, SA promoted the activities of sucrose phosphate synthase (SPS), sucrose synthase (SS) and amylases, while the SA-treated root showed a reduced amylase activity and an unchanged activity in SPS. The changes in various sugar contents resulted in the accumulation of soluble sugars in SA-treated cucumber seedlings, especially non-reducing sugars in roots. These increased sugars could function as osmotic regulators and facilitate water uptake and retention in plant cells, thereby conferring seedlings an enhanced tolerance to salinity stresses caused by NaCl treatment. Taken together, our findings provide an important insight into the effects of SA on sugar metabolism, and a protective mechanism for SA against water deficiency is discussed.     

施氮量对葡萄幼苗叶片蔗糖和淀粉代谢的影响

以1年生"维多利亚"葡萄(Vitis vinifera L.cv.Victoria)幼苗为试材,采用温室盆栽试验,设置4个不同施氮量(按每盆纯氮计)处理(0 mg(CK)、150 mg(T1)、300 mg(T2)和450 mg(T3)),研究了氮肥施用量对葡萄幼苗叶片蔗糖和淀粉含量及其代谢相关酶活性的影响,以期揭示氮肥施用量对主要光合产物积累和代谢的影响。结果表明:施用氮肥能显著提高叶片蔗糖和淀粉含量,显著提高叶片蔗糖磷酸合成酶(SPS)和蔗糖合成酶合成方向(SSs)活性,各处理SPS活性大小为T2>T3>T1>CK,其中T2、T3处理显著高于CK、T1处理,施氮量对SSs活性影响不大;蔗糖合成酶降解方向(SSd)活性明显高于SSs活性,蔗糖合成酶净活力表现为降解蔗糖方向。酸性转化酶(AI)、中性转化酶(NI)活性随时间均呈上升趋势,施氮能显著提高AI活性。不同量氮处理能显著提高淀粉磷酸化酶(SP)活性,施氮减缓α-淀粉酶(α-amy)活性的上升,T2处理增加β-淀粉酶(β-amy)和总淀粉酶活性。综上所述,施氮能增加葡萄叶片蔗糖和淀粉含量,提高蔗糖和淀粉代谢相关酶活性,促进葡萄幼苗碳水化合物的积累和代谢,其中T2处理效果较优。     

Different mechanisms to obtain higher fruit growth rate in two cold-tolerant cucumber (Cucumis sativus L.) lines under low night temperature

One cold-sensitive cultivar (Jinyan 4) and two cold-tolerant inbred lines (NY-1 and XC-1) of cucumber (Cucumis sativus L.) were subjected to temperatures of 28 °C/22 °C (day/night, control) or 28 °C/12 °C (day/night, cold treatment) in a 10 h photoperiod (7:00–17:00). Under control conditions, cucumber fruits grew fast during afternoon and early night, and slow during late night and morning. Under 28 °C/12 °C conditions, the two cold-tolerant inbred lines maintained relatively higher fruit growth rates than the cold-sensitive cultivar by different mechanisms. Compared to Jinyan 4, NY-1 fruits had higher growth rates during cold nights while XC-1 fruits grew faster during the next day. Under the 28 °C/12 °C temperature regime, the assimilate accumulation in the fruits of all tested genotypes followed a similar trend with the corresponding fruit growth rates. After a cold night treatment, the net CO₂ assimilation rates of one- and two-fruit plants, which had increased sink demand, were higher than that of plants without fruits in all tested genotypes. This response indicates that feedback inhibition might be an important reason for the reduction of photosynthesis on the next day. In addition, after a cold night treatment, the levels of exportable sugars (sucrose and stachyose) in mature leaves of XC-1 were higher than those measured in Jinyan 4 and NY-1, which might explain why XC-1 fruits had faster assimilate accumulation rates in the next morning. Higher activity of sucrose-phosphate synthase, a key enzyme of sucrose and stachyose biosynthesis, constituted an additional evidence that faster sucrose and stachyose biosynthesis in mature leaves may occur in XC-1 than in Jinyan 4 and NY-1 at that time. In conclusion, our results showed that cucumber genotypes may use different mechanisms to enhance their cold tolerance.     

Possible role of non-structural carbohydrates in flower induction in strawberry

Summary

Flower-induction is the event that initiates the transition of a vegetative apex into a floral apex in response to an environmental or developmental cue. Under flower-inducing conditions, biochemical or physiological changes can be recognised. One possible change that could occur is in sugar content. In this study, levels of non-structural carbohydrates (e.g., sucrose, glucose, fructose and starch) were measured in shoot tips, leaves and roots of strawberry (Fragaria ananassa cv. ‘Kordestan’) under flower bud-inducing conditions, and compared with non-induced plants. Runner plants were potted and grown for 4 weeks under non-inducing conditions (31º/25°C day/night; 16 h daylength). Half of the plants were then put under flower-inducing conditions (25°/15°C day/night; 8 h daylength) for 3 weeks. Samples for carbohydrate analysis were taken from induced and non-induced plants every 3 d over 3 weeks, and sucrose, glucose and fructose contents were determined by HPLC, and starch concentrations by the anthrone method. The most abundant soluble sugar, in all organs tested, was sucrose. Sucrose levels in shoot tips and leaves decreased at the beginning of the induction treatment, but soon increased to the levels recorded in non-induced plants. Fructose increased markedly in shoot tips of induced plants 3 d after the start of the short-day treatment, and declined thereafter. Starch contents in shoot tips, leaves and roots of non-induced strawberry plants were higher than those in induced plants on most sampling dates. From the results of this study, it appears that soluble carbohydrate contents in different organs of June-bearing strawberry may have a decisive role on flower-bud induction.     

杏果实发育过程中糖积累与蔗糖代谢相关酶的关系

以新世纪杏作为试材,在不同发育时期测定了果实中蔗糖、葡萄糖和果糖的质量分数以及蔗糖代谢相关酶的活性,对果实中糖积累与酶活性的关系进行了分析。结果表明,在果实发育过程中,蔗糖质量分数与蔗糖合成酶的合成方向和蔗糖磷酸合成酶呈极显著和显著相关,相关系数分别为0.934 9**和0.841 8*,而与中性转化酶活性呈显著负相关,相关系数为-0.842 5*。果实硬核期结束是果实中糖积累和蔗糖代谢相关酶的净活性变化的转折时期。硬核期结束之前果实中蔗糖代谢相关酶的净活性为负值,蔗糖积累缓慢;硬核期结束后进入着色期,蔗糖代谢相关酶的净活性转为正值,蔗糖积累迅速。     

甜瓜肌醇半乳糖苷合成酶基因CmGAS1的表达与功能分析

甜瓜（Cucumis melo L.）是棉子糖系列寡糖（Raffinose Family Oligosaccharides,RFOs）转运型植物。肌醇半乳糖苷合成酶（GAS）是催化RFOs合成的关键酶。甜瓜CmGAS1基因（GenBank登录号为AY077642.1）的cDNA全长为1 903 bp,开放阅读框（ORF）为996 bp,编码331个氨基酸。CmGAS1蛋白分子量约为38 kD,理论等电点（pI）为4.81。氨基酸序列比对和系统进化树分析表明,CmGAS1氨基酸序列与黄瓜（AAO84915.1）亲缘关系最近,同源性为98.79%,与西瓜（Cla009222）同源性为97.58%。荧光定量结果表明,甜瓜叶片从幼叶（库）至成熟叶（源）CmGAS1的表达显著升高,第5节位叶片达到最大值。去掉50%叶片植株与对照相比,完全展开功能叶片的CmGAS1表达量明显升高。应用CmGAS1特异性启动子构建CmGAS1的过表达载体,并采用农杆菌介导进行甜瓜遗传转化,获得了PCR阳性转化植株,转化植株完全展开功能叶片的净光合速率以及叶片中的蔗糖、棉子糖、水苏糖含量与野生型相比均有所提高。推测CmGAS1在甜瓜叶片棉子糖系列寡糖合成过程中起重要作用。     

黄瓜叶片和韧皮部汁液中碳水化合物及相关酶的日变化

 以黄瓜叶片和韧皮部汁液为对象, 研究1个昼夜周期中各种碳水化合物含量及相关酶活性的变化。结果表明夜间至上午10: 00, 葡萄糖和果糖是叶片中主要的可溶性糖, 而下午13: 00至16: 00,水苏糖成为成熟叶片中含量最高的可溶性糖, 这种变化主要由叶片中水苏糖含量剧烈的昼夜变化引起; 当黄瓜同化物从源叶向外运输时, 韧皮部汁液中的可溶性糖以水苏糖为主, 运输完毕后则以蔗糖为主; 黄瓜成熟叶片在夜间以淀粉、葡萄糖、果糖、水苏糖、蔗糖等各种形式贮藏尚未运出的同化物, 而此时韧皮部汁液中葡萄糖、果糖、水苏糖的含量极低, 可能有特定的源库调节机制阻止叶片中的可溶性糖在夜间进一步向韧皮部中装载。     

蔗糖代谢相关酶在卡因菠萝果实糖积累中的作用

以菠萝品种卡因为材料,测定了不同发育时期果实中蔗糖、葡萄糖和果糖含量以及蔗糖代谢相关酶-酸性转化酶(AI)、中性转化酶(NI)、蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)的活性,并对果实中糖积累与酶活性的关系进行了分析.结果表明,卡因果实的发育呈单"S型"曲线,在幼果期到果实迅速生长期,果糖和葡萄糖积累较多,蔗糖积累缓慢,此期蔗糖积累较少与高活性蔗糖转化酶有关;进入成熟期,果实中蔗糖迅速积累,而己糖略有上升,果实蔗糖的显著增加主要与SS和SPS活性有关.酸性转化酶和中性转化酶前期活性很强,后期降低;蔗糖合成酶(合成方向)和蔗糖磷酸合成酶的活性变化趋势相似,前期弱后期强.经过相关性分析:果实中蔗糖含量与SS和SPS活性存在极显著的正相关性(相关系数分别为r=0.97273**和r=-0.91004**),而与NI活性呈极显著负相关(r=-0.76419**),与AI的活性呈显著负相关(r=-0.60594*).     

嫁接对网纹甜瓜果实发育、糖含量及蔗糖代谢相关酶活性的影响

 试验研究了嫁接对日光温室栽培的网纹甜瓜果实发育、糖含量及蔗糖代谢相关酶活性的影响。结果表明: 嫁接加快了果实的膨大速度, 但对中果肉厚度影响不大; 降低了网纹甜瓜中果肉内的果糖、葡萄糖含量; 改变了网纹甜瓜蔗糖积累模式, 嫁接网纹甜瓜中果肉内蔗糖积累时间较自根提前了7 d左右,蔗糖含量高于自根; 但嫁接降低了网纹甜瓜中果肉的总糖含量及酸性转化酶(AI) 和中性转化酶(NI) 活性, 提高了蔗糖磷酸合成酶( SPS) 和蔗糖合成酶( SS) 活性。     

砧木对小型早熟西瓜果实糖代谢及相关酶活性的影响

 以小型西瓜品种‘小兰’自根植株为对照, 研究‘杭州长瓠’、‘超丰F₁’、‘勇士’、‘黑籽南瓜’4 种砧木对其果实糖代谢特性及相关酶活性的影响。结果表明: 4 种嫁接西瓜果实发育过程中蔗糖、葡萄糖、果糖含量和糖代谢酶活性变化趋势与自根西瓜一致。在果实发育早期, 果实中以分解酶类为主, 糖分积累低; 发育后期以合成酶类为主, 糖分积累多。西瓜嫁接后果实糖分含量明显降低, 降低程度依砧木而异, 超丰F₁/ 小兰、杭州长瓠/ 小兰西瓜的糖含量明显高于黑籽南瓜/ 小兰、勇士/ 小兰。砧木可能主要通过影响果实发育早期转化酶( Inv) 、发育后期蔗糖磷酸合酶(SPS) 、蔗糖合酶(SS) 活性来影响糖分积累。各处理糖代谢酶的净活性与糖分积累存在较好相关性, 说明糖代谢酶在西瓜果实糖分积累中的作用是综合的, 造成自根及嫁接西瓜果实糖分积累的差异是Inv、SPS、SS 几种酶共同作用的结果。     

水分胁迫期间及胁迫解除后苹果树源叶碳同化物代谢规律的研究

在苹果果实生长早期进行水分胁迫处理，研究水分胁迫期间及胁迫解除之后源叶中的不同种类碳同化物含量及相关酶活性的变化，结果表明，水分胁迫条件下源叶中总可溶性糖和还原糖积累，山梨醇含量升高；前两个干旱胁迫周期源叶中淀粉含量显著降低；水分胁迫对还原糖的影响主要是增加葡萄糖和果糖的含量，葡萄糖含量在中度胁迫时开始增加，严重胁迫时急剧升高，但果糖含量仅在轻度胁迫时显著高于对照，对蔗糖含量的影响较小。水分胁迫解除后，水分胁迫株叶片中除葡萄糖能保持一个显著高于对照的水平外，其余的糖和山梨醇均迅速地回落到正常灌溉的对照水平。同时，树体承受水分胁迫期间，源叶中的6－磷酸醛糖还原酶（A6PR）、山梨醇脱氢酶（SDH）、磷酸蔗糖合成酶（SPS）、蔗糖合成酶（SS）、中性转化酶、酸性转化酶、ADPG焦磷酸化酶（ADPGPPase）和淀粉酶等均有可能得到显著增加，并且水分胁迫解除后各种酶在短期内仍维持一个较高的活性。     

核桃果肉发育过程中糖含量及相关酶活性的变化

 以普通核桃为试材，研究了核桃果肉发育过程中糖含量及其相关酶活性的变化。结果表明:总糖含量呈现逐渐增长趋势，在果实油脂转化期(约花后90 d)达到极大值；淀粉含量呈波动状态。各种可溶性糖含量变化各异，果糖在果实发育前期含量最高，后期有所降低；葡萄糖含量变化不大; 蔗糖含量前期极低，后期逐渐增加。酸性转化酶和淀粉酶前期活性很强，后期降低；蔗糖合成酶(合成方向)和蔗糖磷酸合成酶的活性变化趋势相似，前期弱后期强。     

NaCl胁迫对番茄叶片光合特性及蔗糖代谢的影响

为了从碳水化合物生产及代谢的角度分析盐胁迫影响番茄产量和果实品质的原因,以番茄品种‘辽园多丽’为试材,研究不同浓度的NaCl胁迫处理对番茄光合特性及叶片中糖代谢的影响。结果表明:盐胁迫导致番茄叶片的净光合速率下降,NaCl浓度越大降低得越多;盐胁迫降低了番茄叶片的气孔导度、胞间CO2浓度和蒸腾速率,降低了番茄叶片的叶绿素a、b和总叶绿素含量,这种降低与NaCl浓度正相关。NaCl胁迫导致番茄叶片中果糖和葡萄糖大量积累,NaCl浓度越高积累得越多;NaCl胁迫降低了番茄叶片中的蔗糖和淀粉含量;NaCl胁迫后,番茄叶片中的转化酶活性以及蔗糖合成酶活性均有所提高,而且随着盐浓度增加而增大。说明NaCl胁迫破坏了番茄叶片的光合机能,降低了光合作用效率,而且盐浓度越大降低得越多;NaCl改变了番茄叶片中的糖代谢方向,显著增加了淀粉和蔗糖的分解,提高了叶片中的果糖和葡萄糖含量,而且这种影响随着盐浓度增加而增大。     

番茄果实发育过程中糖的变化与相关酶活性的关系

 在日光温室内以普通栽培番茄(Lycopersicon esculentum Mill. ) ‘辽园多丽’为试材, 在番茄开花后不同天数, 分别取果柄维管束、萼片、果肉、果胶质和胎座、心室隔壁和果实维管束, 分别测定各部位糖的组成和含量以及糖代谢相关酶的活性。结果表明, 在番茄果实发育过程中, 果肉中葡萄糖和果糖的含量与蔗糖合成酶( SS) 活性呈显著和极显著负相关, 相关系数分别为-0.9497^*和-0.9598^**, 两者与转化酶的活性在果实整个发育期达到显著正相关; 而蔗糖的含量与酸性转化酶及中性转化酶活性均达到极显著负相关, 相关系数分别为-0.9706^**和-0.9669^**, 与SS活性呈显著正相关, 相关系数为0.8886^*。在果实整个发育期, 3种糖的含量均与蔗糖磷酸合成酶( SPS) 无显著相关, 说明番茄果实中各种糖的积累与代谢主要受SS和转化酶活性的调控, 与SPS活性关系不大。果实中淀粉的积累只与SS呈显著正相关, 与其它3种酶活性关系不大。所以转化酶与蔗糖合成酶( SS) 的共同作用是影响普通栽培型番茄果实中糖积累的重要因子。