实验室信息管理系统——电子类职业技能培养性实验室信息管理系统设计

目前国内外的实验室信息管理系统产品比较偏重于化工类、科研性质比较重的实验室管理，而电子类、职业技能培养性质的实验室管理产品极少，笔者以重庆电子工程职业学院为模本设计了一套类似实验室均可使用的实验室信息管理系统。文中主要介绍了笔者的设计理念与相关思路。     

量化管理在兽药实验室信息管理系统中的应用

开发了一套适用于兽药与饲料实验室管理的操作简便、实用及扩展性强的量化管理功能组件,应用于广东省兽药与饲料监察总所绩效管理。该组件采用J2EE分布式组件技术,结合量化管理方法,很好地整合到原有的B/S（浏览器/服务器）模式的兽药与饲料实验室管理系统中。应用实践表明,该系统实现了对实验室人员工作状况的全过程管理,促进了检验工作的规范化、流程化,提高了工作效率,保证了人员绩效考核的公开、公平、公正。     

Data standardization implementation and applications within and among diagnostic laboratories: integrating and monitoring enteric coronaviruses

Every day, thousands of samples from diverse populations of animals are submitted to veterinary diagnostic laboratories (VDLs) for testing. Each VDL has its own laboratory information management system (LIMS), with processes and procedures to capture submission information, perform laboratory tests, define the boundaries of test results (i.e., positive or negative), and report results, in addition to internal business and accounting applications. Enormous quantities of data are accumulated and stored within VDL LIMSs. There is a need for platforms that allow VDLs to exchange and share portions of laboratory data using standardized, reliable, and sustainable information technology processes. Here we report concepts and applications for standardization and aggregation of data from swine submissions to multiple VDLs to detect and monitor porcine enteric coronaviruses by RT-PCR. Oral fluids, feces, and fecal swabs were the specimens submitted most frequently for enteric coronavirus testing. Statistical algorithms were used successfully to scan and monitor the overall and state-specific percentage of positive submissions. Major findings revealed a consistently recurrent seasonal pattern, with the highest percentage of positive submissions detected during December–February for porcine epidemic diarrhea virus, porcine deltacoronavirus, and transmissible gastroenteritis virus (TGEV). After 2014, very few submissions tested positive for TGEV. Monitoring VDL data proactively has the potential to signal and alert stakeholders early of significant changes from expected detection. We demonstrate the importance of, and applications for, data organized and aggregated by using LOINC and SNOMED CTs, as well as the use of customized messaging to allow inter-VDL exchange of information.     

Some aspects of erythrocyte metabolism in a dog with polycythaemia vera

An 11-year-old female crossbred dog showed signs of polyuria, polydipsia, vomiting, posterior weakness and ataxia. Clinical and laboratory findings suggested the diagnosis of polycythaemia vera. The haematological values shown over a six-month period are presented. In four samples some aspects of erythrocyte function (glucose-6-phosphate dehydrogenase [G6PD] and pyruvate kinase [PK] activities, 2,3 diphosphoglycerate [2,3 DPG] concentration, osmotic fragility and intracellular sodium and potassium concentrations) were studied. Variable activities of G6PD and PK, probably related to different reticulocyte number, were detected together with normal osmotic fragility and intracellular sodium and potassium concentrations. 2,3 DPG concentration was higher than normal in all four samples. This could be interpreted as a response to a low tissue perfusion rather than a higher content of 2,3 DPG in red blood cells from the polycythaemic dog.     

基于B/S模式的实验室信息管理系统的构建与应用

分别从检验检测管理、检测资源管理、日常办公、信息交流、系统管理及统计与报表等6个方面构建了实验室信息管理系统,对饲料、兽药和残留检验实验室进行协调统筹科学的管理。该系统可大大提高工作效率,缩短检测周期并降低检测成本,即实现了对检测过程全程监控管理,又实现了量化管理自动化。实践应用结果表明:实验室信息管理系统是实现实验室规范化科学管理的有力工具。     

A proposal to leverage high-quality veterinary diagnostic laboratory large data streams for animal health,public health,and One Health

Test data generated by ~60 accredited member laboratories of the American Association of Veterinary Laboratory Diagnosticians (AAVLD) is of exceptional quality. These data are captured by 1 of 13 laboratory information management systems (LIMSs) developed specifically for veterinary diagnostic laboratories (VDLs). Beginning ~2000, the National Animal Health Laboratory Network (NAHLN) developed an electronic messaging system for LIMS to automatically send standardized data streams for 14 select agents to a national repository. This messaging enables the U.S. Department of Agriculture to track and respond to high-consequence animal disease outbreaks such as highly pathogenic avian influenza. Because of the lack of standardized data collection in the LIMSs used at VDLs, there is, to date, no means of summarizing VDL large data streams for multi-state and national animal health studies or for providing near-real-time tracking for hundreds of other important animal diseases in the United States that are detected routinely by VDLs. Further, VDLs are the only state and federal resources that can provide early detection and identification of endemic and emerging zoonotic diseases. Zoonotic diseases are estimated to be responsible for 2.5 billion cases of human illness and 2.7 million deaths worldwide every year. The economic and health impact of the SARS-CoV-2 pandemic is self-evident. We review here the history and progress of data management in VDLs and discuss ways of seizing unexplored opportunities to advance data leveraging to better serve animal health, public health, and One Health.     

Analysis of a practice management computer software program for owner compliance with recall reminders

The purpose of this study was to describe clients' level of compliance with reminders that are computer generated and to identify factors associated with owner response to a recall. Client response to a recall notice revealed that clients responded to reminders for vaccination about 3 times less often than for the other recall codes combined (dental procedures, laboratory tests, medical progress examinations, and neutering). These results suggest that veterinarians need to move away from annual vaccination reminders and toward the promotion of preventive veterinary services, such as annual wellness examinations, screening laboratory testing, and routine dental procedures. Once a practice has committed to and set up the required computer systems to promote preventive veterinary care, the stage is set for the implementation and monitoring of recommendations and reminders. This type of practice philosophy and marketing coincides perfectly with an approach to maximizing compliance in all aspects of veterinary medicine.     

Rabbit Clinical Pathology

With rabbit patients, as in other species, analyzing blood and urine samples can be useful and informative, although interpretation of the results is sometimes challenging. This article summarizes the interpretation of laboratory results from rabbits. Hematological parameters can yield information about the red blood cell population and leukocyte response to stress and pathogens. Biochemistry evaluation can be used to investigate liver, kidney, and other organ function, and urinalysis results may yield additional information about kidney function and electrolyte imbalances. Serological tests are available for several pathogens of rabbits, including Encephalitozoon cuniculi, although the significance of positive results and antibody titers is not clear. Serum protein electrophoresis aids the understanding of protein disorders and the immune response to acute and chronic inflammation.     

Physical and clinical pathological findings associated with experimentally induced rupture of the equine urinary bladder

Two mature horses were examined for changes in laboratory and physical findings after experimentally induced bladder rupture. The postrupture laboratory diagnostic changes, which provide valuable information for a correct diagnosis are described. Hematology, serum and peritoneal fluid sodium, potassium, calcium, phosphorous, creatinine, urea nitrogen, albumin and peritoneal fluid components were measured and evaluated versus time. Hyponatremia and hyperkalemia occurred, as well as increased concentrations of peritoneal fluid potassium and inorganic phosphorus. In addition, peritoneal fluid creatinine:serum creatinine and peritoneal fluid urea nitrogen:serum urea nitrogen ratios were followed with time. Hematology and cytology of the peritoneal fluid showed an inflammatory response to urine contamination of the abdominal cavity. Physical findings of tachypnea and tachycardia as well as a mild colic were absent until nearly 50 hours postrupture. Based on these findings, it was concluded that the peritoneal fluid creatinine:serum creatinine ratio was the most useful antemortem laboratory diagnostic aid.     

Species-specific primate husbandry

With about 300 species primates represent one of the largest animal groups within mammals. They are kept in zoological gardens, as laboratory animals or in private ownership. Against this background it is difficult to define all species-specific aspects of a primate husbandry. The paper describes the basic requirements for primates like nutrition, possibilities for social interactions and species specific behaviour, stimuli for activities, health care and environmental conditions. Although no definitive regulations exist by law, the Appendix A (Species-specific Provisions for Non-human Primates) of the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (ETS 123) will be of great importance in the future.     

Research Progress of Fatty Acid Regulation of microRNAs in Dairy Cow Mammary Glands

Lipids are a kind of substance which can store and provide energy for animals. A large number of studies have confirmed that the change of lipids in diets can alter the expression of microRNAs (miRNAs) and thus affect the biological activities of the bodies. This review mainly summarizes the regulation of fatty acids on the expression of miRNAs in mammary gland of dairy cows, from the aspects of bovine mammary miRNAs expression characteristics, miRNAs that regulate milk fat synthesis, and the changed bovine mammary miRNAs in response to exogenous addition of fatty acids. All these works may be beneficial for the establishment of the connection between nutrients and mammary function, and provide information for the related research in the future.     

脂肪酸调控奶牛乳腺microRNAs研究进展

脂类是一种可以为动物体储存和提供能量的物质,大量研究证实,日粮中脂类的改变可以改变microRNAs（miRNAs）的表达从而影响机体生命活动。本综述主要概括了脂肪酸对奶牛乳腺miRNAs的调控作用,从奶牛乳腺miRNAs表达特征、调控乳脂合成的miRNAs及外源添加脂肪酸对乳腺miRNAs的调控三个方面进行概述,旨在从转录后调控角度,建立营养物质与乳腺功能的联系,为未来相关研究提供参考。     

Noncomminuted fractures of the proximal phalanx in 69 horses

In a retrospective study, we examined the case records for 69 horses with noncomminuted fractures of the proximal phalanx, excluding proximal chip fractures. Forty-nine of the horses sustained the fracture while racing or training for racing. Radiographic examination of all affected limbs was performed, and fractures were classified into 6 noncomminuted types: midsagittal fractures, including short incomplete midsagittal fractures, long incomplete midsagittal (LIMS) fractures, and complete midsagittal (CMS) fractures; dorsal frontal fractures; distal joint fractures; plantar process fractures; physeal fractures; and oblique fractures. Four horses were destroyed before treatment. Sixty-five horses were treated and 63 survived to go home. Long-term follow-up evaluation of the horses that were sent home revealed that 4 were euthanatized after discharge because of persistent lameness of the fractured limb. Of the 59 remaining horses, 34 returned to racing, 7 were used as show or pleasure riding animals, 8 were used for breeding, and 10 were lost to follow-up. More Standardbreds with noncomminuted proximal phalangeal fractures returned to racing than did Thoroughbreds. Of 30 Standardbreds that raced or trained before the fracture, 23 (76.7%) returned to racing--8 to their previous level of performance, 11 to a lower level of performance, and 4 to an unknown level of performance. The Standardbreds that returned to racing were horses with physeal fractures (2/2), LIMS fractures (4/4), CMS fractures (11/16), short incomplete midsagittal fractures (4/7), distal joint fractures (1/2), and plantar process fractures (1/2). Of 21 Thoroughbreds that raced or trained before the injury, 11 (52.4%) returned to racing--7 to their previous level of performance and 4 to a lower level of performance.(ABSTRACT TRUNCATED AT 250 WORDS)     

In vivo measurement of bone strain in the horse.

Strain gauges were successfully bonded in vivo to the cranial, caudal, medial, and lateral aspects of the equine radium and tibia and to the dorsal, palmar, or plantar, medial, the lateral aspects of the metacarpus and metatarsus--all in the mid-diaphyseal region. Various activities were investigated, including walking, trotting or pacing, and standing up from anesthesia. The strain patterns showed that each stride produced a characteristic deformation cycle. The strains were measured and the axial loads were calculated as the horse performed certain activities. The tension band side of each bone was predicted from the results. The tension band sides of the metacarpus and metatarsus were the dorsomedial and dorsolateral aspects; for the radius and tibia, the tension band sides were the cranial and craniolateral aspects, respectively.     

Determination of clotting factor VIII activity in mammals using the one-stage clotting and the chromogenic methods

Two different laboratory assays exist for the determination of clotting factor VIII activities in human plasma. One method is based on the one-stage clotting principle, the other one on the chromogenic principle. To evaluate the suitability for veterinary medicine human plasma and plasma of ten mammalian species (pig, cattle, dog, cat, zebra, llama, snow leopard, greater kudu, horse-like antelope, mink) were examined with each method. Factor VIII activities in human plasma determined using both assays were in a good agreement. Results of factor VIII activities in plasma of mammals obtained with both methods were similar, if a species-specific reference plasma was used. The practicability of the chromogenic method was reduced. However, the factor VIII activities differed nonsystematically up to 500% between both methods, if a human plasma was used as a reference plasma. Determination of factor VIII activities in plasma of mammals using the chromogenic assay cannot be recommended.     

Effects of larval size and hydrodynamics on the growth rates of the black fly Simulium tribulatum

Black flies are ubiquitous and important members of lotic ecosystems. Size is known to affect many aspects of their life in the aquatic larval stage, including intraspecific competition for feeding sites. As filter feeders, flow affects their ability to feed and reach sufficiently fast flow. This, in turn, can lead to risky fluid‐mediated dispersal behavior in search of better conditions. It is surprising, therefore, that little information is available regarding how physiological and environmental factors combine to affect larval growth rates. The present study determines the relative growth rates of small (0.6 mm) and large (approximately 4 mm) larvae in laboratory flumes designed to produce spatially homogeneous and temporally consistent flow regimes at ecologically relevant velocities (44 and 64 cm/s). Our results indicate that size and flow both influence growth rates and that the 2 interact significantly. Young larvae exhibit faster growth rates and a greater positive response to increased flow speed. This result might help explain why smaller larvae have a greater propensity to disperse than larger larvae: the benefit of increased growth rate that they receive from relocating to faster flow might balance the risks inherent in dispersal.     

植物精油抗菌活性研究进展

植物精油来源于天然,具有抑菌、杀虫、抗氧化等活性,得到了广泛应用。笔者从植物保护、医药卫生、食品保存3个方面综述了近年来国内外植物精油抗菌活性研究进展,以期为开发植物精油提供参考。     

Anaesthesia and analgesia of the donkey and the mule

The number of donkeys and mules throughout the world is stable, and awareness of their use and concern for welfare, pain recognition and treatment are receiving increasing veterinary interest. Therefore, accurate information about anaesthesia and analgesia in donkeys and mules is important to ever more equine practitioners. Since donkeys are physiologically and pharmacologically different from horses, knowledge on species specific aspects of anaesthesia and analgesia are very important. Mules combine elements from both donkey and horse backgrounds, leading to great diversity in size, temperament and body type. Physiologically, they seem to resemble horses more than donkeys. This review highlights the current knowledge on various anaesthetic and analgesic approaches in donkeys and mules. There is still much information that is not available about donkeys; in many circumstances, the clinician must use available equine information to treat the patient, while monitoring carefully to observe for differences in response to therapy compared to the horse.     

Index Lists provided by the VAMPP computer programme for dairy herd health and production control. IV. Interpretation and analysis of data on Index List 4

In a series of 4 papers computerized herd reports on various aspects of dairy farming, the Index Lists, are presented for herd health and production control purposes. This fourth paper regards Index List 4 on nutritional disorders, body condition and ration composition. The information from this list is suitable to monitor nutritional efficiency and feeding management, especially when used in combination with information from the other index lists. Methods for interpretation and evaluation of the data listed are provided. Evaluations should take into account the aspects related to nutrition. It is demonstrated how deviations of index figures are detected and how advice to farm management is built up by means of actual information and prompt computerized analysis.     

Index LISTS provided by the VAMPP computer programme for dairy herd health and production control

Summary

In a series of 4 papers computerized herd reports on various aspects of dairy farming, the Index Lists, are presented for herd health and production control purposes. This fourth paper regards Index List 4 on nutritional disorders, body condition and ration composition. The information from this list is suitable to monitor nutritional efficiency and feeding management, especially when used in combination with information from the other index lists.

Methods for interpretation and evaluation of the data listed are provided. Evaluations should take into account the aspects related to nutrition. It is demonstrated how deviations of index figures are detected and how advice to farm management is built up by means of actual information and prompt computerized analysis.