窗纱式机械化生产白僵菌孢子粉工艺的研究

窗纱式机械化生产白僵菌(Beauveria bassiana)孢子粉的工艺是:培养料在搅拌消毒罐中高压灭菌,接种后制成菌浆,共把它涂在上浆机一种特制的窗纱上形成菌膜片,送到培养室定温保湿培养,待白僵菌孢子成熟以后,用刷粉机把窗纱片上的白僵菌孢子粉刷集下来,烘干成白僵菌孢子粉剂(含孢量1500～2500亿/g)冷藏备用。     

推求含水层参数的一种新方法 —S—lnr直线趋势模拟法

本文提出了推求含水层参数的S－lnr直线趋势模拟法的基本理论，它可用于满足泰斯假设条件的承压非稳定流抽水试验及满足雅格布假设条件的潜水非稳定流抽水试验含水参数推求上，并通过实例与传统求参方法所得结果进行对比分析，结果表明：S－lnr直线趋势模拟法是比较精确的一种求参方法。     

甘薯两次嫁接法诱导开花和结实的研究

甘薯是短日照植物,在我国北方秋季日照较长,一般品种不能开花。用大牵牛花为砧木,天然开花的品种为中间砧木,杂交亲本为接穗,经过两次嫁接后,能诱导甘薯一般品种在北方地区自然开花,且效果显著。应用此法可更广泛地进行甘薯有性杂交育种。接穗品种约在嫁接后12天左右开始现蕾,30天左右开始开花,80天左右个别品种开始停止开花,到100天左右全部品种停止开花。接穗品种间现蕾,开花两期差异较小,现蕾最早者比最晚者早6天,开花最早者比最晚者早2天。停花期各品种之间差异显著,最早者比最晚者提前20天。各接穗品种盛花期在8月10日～9月4日之间,结实盛期在8月30日～9月18日之间,在上述两期中,一般开花越多,结实率越高,接穗品种开花数目和结实率因品种不同而异;开花较多者(蓬尾),平均每株为292.3朵,较低者(华北553),平均每株为90.3朵;结实率较高者(徐薯18),为53.7%,较低者(蓬尾),为24.3%。开花数目与结实率呈反比关系,开花越多,结实率越低。     

浙农大A系意蜂王浆高产试验

 以王浆高产为主要特征的浙农大A系意峰20群，通过1年定地结合小转地饲养，运用适合浙农大A系意蜂的多项生产技术，全年共产王浆154.1kg，平均每群产浆7.7 kg，刷新了国内外王浆高产记录；同时，生产蜂蜜950 kg，平均每群47.5 kg；花粉35 kg，平均每群1.75 kg；蜂蜡17 kg，平均每群0.85 kg；蜂群发展到60群，增加了2倍。全年群均创利739.1元，创造了我国群均效益的最高记录。     

药物美沙拉秦合成工艺的改进

合成新药5-胺基-2-羟基苯甲酸（美沙拉秦）。以邻羟基苯甲酸的钠盐为原料,在超声波作用下合成药物5-胺基-2-羟基苯甲酸。通过与文献报道路线的对比,可知在超声波作用下的合成路线能够节省时间,提高回收率等优点。此法适用于合成新药5-胺基-2-羟基苯甲酸。     

PRODUCTION OF NEW WAP-8294A CYCLODEPSIPEPTIDES BY THE BIOLOGICAL CONTROL AGENT LYSOBACTER ENZYMOGENES OH11

● Lysobacter enzymogenes mutants were generated for WAP-8294A biosynthesis. ● Essential and non-essential accessory genes for WAP-8294A biosynthesis were determined. ● Six new WAP-8294A analogs were identified using UHPLC-HR-MS/MS. ● Three deoxy analogs were detected supporting the function of ORF4 in asparagine hydroxylation. Naturally-occurring environmental microorganisms may provide ‘green’ and effective biocontrol tools for disease management in agricultural crops. Due to the constant threat of resistant pathogens there is a pressing and continual need to search for new biocontrol tools. This study investigated the production of new analogs of WAP-8294A compounds by the biocontrol agent Lysobacter enzymogenes OH11 through biosynthetic engineering. WAP-8294As are a family of natural cyclic lipodepsipeptides with potent activity against Gram-positive bacteria. A series of genetic manipulations was therefore conducted on the accessory genes in the WAP biosynthetic gene cluster. The resulting strains containing a single-point mutation in ORF4, which was predicted to encode a 2-ketoglutarate dependent dioxygenase, produced deoxy-WAP-8294As. This result provides evidence for the function of ORF4 in catalyzing β-hydroxylation of the D-asparagine residue in WAP-8294As. In addition, six new analogs of WAP-8294As were identified by UHPLC-HR-MS/MS. This is the first attempt to produce new WAP-8294As in Lysobacter and shows that the spectrum of the biocontrol compounds may be expanded through the manipulation of biosynthetic genes.