Association analysis of FcεRⅠβ gene with allergic asthma in Chinese

AIM: To investigate the linkage between the polymorphism of -109 and Glu237 in the high-affinity IgE receptorβ(FcεRⅠβ) gene and susceptibilty of allergic asthma in adults in a Chinese population. METHODS: Allergic asthma sample in adult and age-and sex-matched control were studied. A-109C/T and a coding variant Glu237Gly in FcεRⅠβ were detected with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: (1)The genotype frequencies were 0.415 for -109T/T, 0.491 for-109T/C and 0.094 for -109C/C in allergic asthma in adults. No significant difference in the distribution of-109C/T polymorphism was found between allergic asthma adult subjects and healthy control subjects. However, a homozygosity for the-109T allele was associated with increased total plasma IgE levels in subjects with allergic asthma (F=7.213, P<0.01). (2)The allele frequency of Gly237 in the patients and control was 0.245 and 0.142, respectively. There was a significant association between Gly/Gly genotype and allergic asthma in adults among allergic asthma patients. Gly237 was significantly associated with high IgE. CONCLUSIONS: It suggests that Gly237 vaviant of the FcεRⅠβ gene is involved in the development of allergic asthma. The-109C/T and Glu237Gly polymorphisms are two of the genetic factors identified, which affects total plasma IgE levels in adult allergic asthma in Han of Hubei province.     

Association of IFN-γ and IL-4 gene polymorphisms with childhood bronchial asthmatic susceptibility and the levels of plasma IFN-γ,IL-4 and IgE

AIM: To investigate the gene polymorphisms of interferon-γ(IFN-γ) and interleukin-4(IL-4) and the association with asthmatic susceptibility and the levels of plasma IFN-γ, IL-4 and IgE of asthmatic children. METHODS: 100 asthmatic children and 122 control children were enrolled the study. The genotypes of IFN-γ gene-179G/T polymorphism, IL-4 gene-33C/T and-589C/T polymorphisms were tested by PCR-RFLP.The genotype of IFN-γ gene +874A/T polymorphism was tested by AS-PCR.The CA repeat polymorphism of IFN-γ gene was detected by capillary electrophoresis technique.The levels of serum IFN-γ, IL-4 and IgE were measured by ELISA. RESULTS: 100 asthmatic children and 122 control children were all GG homozygotes at -179 locus of IFN-γ gene.-179 locus of IFN-γ gene has no mutation. The genotypes and allele frequency of IFN-γ gene +874A/T and CA repeat polymorphisms showed no significant difference between asthmatic children and the control(P>0.05). An association was revealed between IFN-γ gene +874A/T polymorphism and the level of plasma IFN-γ.The level of IFN-γ was lower in AA genotype than in AT genotype(P<0.05). The genotypes and allele frequency of IL-4 gene -33C/T and -589C/T polymorphisms showed significant difference between asthmatic children and the control(P<0.05).The levels of plasma IL-4 and IgE were higher in TT genotype at -33 locus and -589 locus than those in CT genotype, but only -33C/T polymorphism was associated with the level of plasma IL-4(P<0.05). CONCLUSION: The IFN-γ gene +874A/T and CA repeat polymorphisms were not correlated with asthmatic susceptibility, but there is significant correlation between the level of IFN-γ and +874A/T polymorphism. TT genotype of IL-4 gene -33 locus and -589 locus maybe the susceptible genotype of asthma in children, and the -33 locus polymorphism is associated with the level of IL-4.     

Expression of IL-1α and TNF-α modified by Toll-like receptor 4 genetic polymorphism is associated with colorectal carcinoma

AIM: To investigate the association of D299G, T399I and A896G polymorphisms of Toll-like receptor 4 (TLR4) and colorectal carcinoma (CRC). METHODS:
The genotypes of these 3 loci among 268 patients with CRC and 268 healthy controls were determined by polymerase chain reaction-restriction fragment lengthy polymorphism (PCR-RFLP). The protein levels of IL-1α, IL-8, TGF-β and TNF-α in the homogenate of CRC biopsies were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: No significant difference of the genotype frequencies of TLR4 A896G and D299G between the cases and the controls was observed. CT combined TT genotype of T399I was significantly associated with increased CRC risk. The individuals with the T allele of T399I showed a 1.843-fold increase in CRC risk as compared with the C allele. The concentrations of IL-1α and TNF-α in CRC biopsies were significantly elevated in the individuals with the genotype of T399I CT combined with TT as compared with the genotype of CC. CONCLUSION: TLR4 T399I promotes the development of CRC by modifying the expression of IL-1α and TNF-α in CRC tissues.     

Levels of IL-17, IL-8 and VEGF in bronchoalveolar lavage fluid in asthma children

AIM: To investigate the mechanism of airway inflammation in children with asthma by determining the levels of IL-17, IL-8 and vascular endothelial growth factor(VEGF) in bronchoalveolar lavage fluid (BALF). METHODS: Eighty-eight children were enrolled in the study and divided into asthma group (n=52), pneumonia group (n=25) and control group (n=11). BALF were collected from all 88 cases. The levels of IL-17, IL-8, VEGF, IL-4 and IFN-γ in BALF were measured by ELISA. The cell types in BALF were determined. RESULTS: Compared with the control, the levels of IL-17 and IL-8 were significantly elevated in asthma group and pneumonia group (all P<0.05). The level of IL-8 (P<0.05) in the patients with asthma was lower than that in the pneumonia patients. No statistical difference of the IL-17 level between asthma group and pneumoniae group was observed (P>0.05). Compared with pneumonia group and control group, the level of VEGF was significantly increased in asthma group (all P<0.01), and the VEGF level among control group and pneumonia groups was almost similar (P>0.05). The levels of IL-4 and IFN-γ, and ratio of IL-4/IFN-γ among groups were not statistically different. The percentage of neutrophils in BALF was significantly higher in asthma group and pneumonia group than that in control group (all P<0.01). CONCLUSION: IL-17, IL-8 and VEGF play important roles in airway inflammation in children with asthma. Th17 cells may participate in the pathogenesis of asthma in children.     

Effects of specific immunotherapy on allergic rhinitis children accompanied with asthma

AIM: To explore the effects of Dermatophagoides pteronyssinus allergen-specific immunotherapy (SIT) on the serum interleukin (IL)-13,IL-4,interferon (IFN)-γ, nasal symptoms and pulmonary functions in allergic rhinitis children accompanied with asthma. METHODS: Fifty-eight cases of allergic rhinitis children accompanied with asthma participated in this study. Their allergens were Dermatophagoides pteronyssinus. Thirty-five children received SIT were SIT group, and the other 23 children received local glucocorticoid treatment were medical group. The serum levels of IL-13, IL-4 and IFN-γ were examined, and the nasal symptoms and pulmonary functions were checked before treatment and one year after treatment. RESULTS: There was a significant difference in nasal symptoms between the two groups one year after treatment (P<0.05). The patients in SIT group had fewer symptoms. The serum levels of IL-4 and IL-13 were clearly reduced. IFN-γ and the ratio of IFN-γ/IL-4 were significantly increased (P<0.05). The pulmonary functions were significantly improved in SIT group (P<0.05). Meanwhile in medical group, the serum levels of IL-4 and IL-13 had less change (P>0.05), and the pulmonary functions were poorly improved (P>0.05). CONCLUSION: SIT may regulate the imbalance of Th1/Th2 cells in allergic rhinitis accompanied with asthma by reducing the serum levels of IL-4 and IL-13 and increasing IFN-γ and the ratio of IFN-γ/IL-4, resulting in reducing the nasal symptoms and improving the pulmonary functions.     

IgE-FcεRI crosslink accelerates atherosclerosis development in allergic asthmatic mice

AIM:To investigate whether allergic asthma accelerates the development of atherosclerosis in mice related to Th2 cells and interleukin-4 (IL-4), and the roles of activation of macrophages by immunoglobulin E (IgE)-Fc ε receptor I (FcεRI) crosslink during the process. METHODS:Six-week-old ApoE^-/- mice were sensitized and challenged by ovalbumin to establish the allergic asthma model, and then assigned to 3 groups:control group, asthmatic placebo group and asthmatic IL-4 monoclonal antibody (mAb) intervention group (intervention for 8 weeks). The lesion area was measured by oil red O staining. The percentages of Th2 cells in the splenocytes of the mice were analyzed by flow cytometry. The mRNA expression of IL-4 and the macrophage-related inflammatory factors, monocyte chemotactic protein 1 (MCP-1), macrophage inflammatory protein-1α (MIP-1α) and IL-6, in the spleen was detected by real-time PCR. Local IgE and FcεRIα expression in the plaque was evaluated by immunofluorescence/immunohistochemical staining, and the circulating IL-4 and IgE were measured by ELISA. RESULTS:Accompanied by aggravated atherogenesis in asthmatic ApoE^-/- mice, the proportion of Th2 cells and IL-4 mRNA in the spleen, IgE and FcεRIα expression in the aortic root, and the mRNA expression of MCP-1, MIP-1α and IL-6 were markedly increased. After 8-week treatment with IL-4 mAb, the lesion area in the aortic root of asthmatic ApoE^-/- mice was markedly decreased, the elevated IgE and FcεRIα expression was significantly decreased, and the mRNA expression of macrophage-related inflammatory factors was also decreased. CONCLUSION:Allergic asthma accelerates the atherosclerosis in ApoE^-/- mice, which is associated with the increased Th2 cells and IL-4, and the activation of macrophages by IgE-FcεRI crosslink.     

The expression and effect of IL-4 in human palatine tonsil

AIM: To study the allergic disease in recurrent tonsillitis. METHODS: Immunohistochemical technique was used to investigate interleukin-4(IL-4) production in tonsil. RESULTS:The result showed that: a higher incidence of IL-4 producing cells were found in the tonsils of all ages, and a high density of IL-4 was found in the reticular crypt epithelium and extrafollicular, but low density in germinalcenter. IL-4 has been shown play a crucial role in the pathogensis of allergic disease. CONCLUSION:Allergic disease is an important reason of recurrent tonsillitis. The conclusion will give a new way to cure recurrent tonsillitis.     

Inhibitory effect of triptolide on production of IL-1β from PBMC is associated with IL-1β gene polymorphism

AIM: To explore whether the inhibitory effect of triptolide on IL-1β production by PBMC is associated with IL-1β gene polymorphisms. METHODS: IL-1β gene polymorphism was analyzed in 31 healthy volunteers. From genomic DNA, the C-T polymorphism at IL-1β-511 was typed by PCR-RFLP. Meanwhile the IL-1β was also measured in the supernatants of the cultured and stimulated peripheral blood mononuclear cells (PBMC) by ELISA. RESULTS: After LPS stimulation in PBMC cultures of healthy subjects, the secretion levels of IL-1β in 9 volunteers who carried IL-1β-511 T/T genotype were higher than in volunteers who are not T/T genotype (P<0.05). Triptolide suppressed the production of IL-1β significantly in LPS-treated human PBMC carried C/C and C/T genotype (P<0.05), but this significant inhibitory effect of triptolide was not seen in T/T genotype (P>0.05). CONCLUSION: The gene polymorphism at IL-1β-511 was related to the production of IL-1β, and the inhibitory effect of triptolide on the production of IL-1β was different in C/C, C/T, T/T genotype of IL-1β-511, which may be one of the reasons for the phenomenon that people respond differently to triptolide.     

Correlation of adiponectin gene SNP+45 T/G polymorphism with coronary heart disease

AIM: To investigate the relationship between adiponectin gene SNP+45 polymorphism and coronary heart disease (CHD) in south China Han population. METHODS: The nondiabetic CHD patients diagnosed by the coronary angiography were selected as CHD subjects (153 cases), and 73 healthy adults served as normal control subjects. The polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) was performed to identify the distribution pattern of adiponectin gene SNP+45 in all subjects. The levels of plasma adiponectin were measured by ELISA. RESULTS: The frequency of T/G + G/G genotype and G allele in CHD patients were significantly higher than those in control subjects (P<0.05). Logistic regression analysis revealed that the adiponectin gene SNP+45 T/G+G/G genotype had a strong positive association with CHD (OR: 2.132, 95.0% CI: 1.034-4.397, P＜0.05). The plasma adiponectin was negatively associated with CHD (OR: 0.868, 95.0% CI: 0.785-0.959, P<0.05). CONCLUSION: The T/G+ G/G genotype was a possible risk factor for CHD in southern China Han population.     

IL-1B-511 single nucleotide polymorphism and gastric atrophy in high prevalence regions of gastric cancer in China

AIM: To study the relationship between IL-1B-511 single nucleotide polymorphism,H.pylori infection,and gastric atrophy in high prevalent (Shanxi) region in China.METHODS: Five hundred healthy volunteers from Shanxi Province of China were recruited in this study,which were divided into five subgroups according to age,namely age 20-29,30-39,40-49,50-59 and >60 years,respectively.Genomic DNA was extracted from peripheral blood.IL-1B-511 single nucleotide polymorphism was analyzed by PCR-RFLP.Serum pepsinogen was used as a biomarker of gastric atrophy.Serum pepsinogen Ⅰ (PGⅠ),pepsinogen Ⅱ (PGⅡ) and anti-H.pylori IgG were determined by an ELISA assay.RESULTS: The mean serum PGⅠ concentration and ratio of PGⅠ /PGⅡ decreased gradually with increasing age,and were lower in subjects with IL-1B-511 T/T genotype and H.pylori infection than those without H.pylori infection in each subgroup,respectively (All P<0.05).Multiple linear regression showed that IL-1B-511 T/T genotype,age and Hp infection were significantly associated with gastric atrophy (P<0.05,P<0.05 and P<0.01,respectively).CONCLUSION: Gastric atrophy is closely correlated with IL-1B-511 T/T genotype,age,H.pylori infection in high prevalence region of gastric cancer in China.The risk of gastric atrophy is significantly increased for the IL-1B-511 T/T genotype with Hp infection.     

Effect of IL-4, CD40L on RANTES production in murine renal tubular epithelial cells

AIM: To investigate the effect of IL-4, CD40L on RANTES production in murine renal tubular epithelial cells (TEC). METHODS: TEC were obtained from mouse, expression of RANTES and CD40 on TEC were measured. RESULTS: (1) Activation of TEC with IL-4 resulted in significant increase in CD40 expression (P＜0.01).(2) A little RANTES was detectable in supernatants without stimulation. TEC stimulated with either cytokine IL-4 or CD40mAb resulted in strong induction of RANTES production up to 43.61±13.73 or 73.77±4.28(ng/L), respectively. The differences of RANTES between two stimulation groups and that in medium were statistically significant (P<0.01). TEC stimulated with IL-4 and CD40mAb produced more RANTES than that in medium (P<0.01), which was higher than that with single stimulation (P<0.01). (3) TEC stimulated with IL-4 or CD40 activation or combined stimulation of IL-4 and CD40mAb resulted in increase in levels of RANTE mRNA, which were higher than that in medium. CONCLUSION: Co-stimulation of TEC by IL-4 and CD40mAb up-regulated the RANTES production, suggesting the RANTES may participate in the inflammation of TEC.     

Relationship between polymorphism of angiotensin I converting enzyme gene insertion/deletion and ACE,PAI-1 activity in patients with myocardial infarction

AIM:To explore the relationship between polymorphism of angiotensin I converting enzyme gene insertion/deletion (I/D) and ACE, PAI-1 activity in patients with myocardial infarction (MI). METHODS:Ninety-three patients with MI and eighty-seven healthy controls were tested. ACE genomic DNA was amplified using the polymerase chain reaction (PCR). Serum ACE activity was measured by colorimetry, plasma level of PAI-1 activity was determined by spectrophotometric assay. RESULTS:① The frequency of ACE DD genotype and D alleles (32.3% and 54.3%) in MI group was significantly higher than those in control group (12.6% and 37.4%, P<0.01, respectively). ② The ACE activity in serum (216.00±58.26)U/L and plasma PAI-1 activity (0.85±0.19)AU/mL in MI group were significantly higher than those in control group (170.19±48.99)U/L, (0.66±0.20)AU/mL, P<0.01, respectively. The serum ACE activity was positively correlated with plasma PAI-1 activity both in MI group and control group (r=0.7108 and r=0.7829;P<0.01, respectively). ③ In MI group, the serum ACE activity and plasma PAI-1 activity showed a significantly higher level in subjects with DD genotype (251.64±57.76)U/L, (0.96±0.16)AU/mL than those with ID (211.47±51.87)U/L, (0.82±0.18) AU/mL and Ⅱ genotypes (179.84±52.65)U/L, (0.71±0.17)AU/mL. The serum ACE activity and plasma PAI-1 activity were significantly higher in subjects with ID genotype than those with II genotype (P<0.05). In control group, the serum ACE activity and plasma PAI-1 activity showed a significantly higher level in subjects with DD genotype (195.53±54.76)U/L, (0.78±0.20)AU/mL than the subjects with Ⅱ genotype (154.98±52.74)U/L, (0.59±0.17)AU/mL (P<0.05). CONCLUSION:The increased ACE activity caused by DD polymorphism may play an important role in elevating the level of plasma PAI-1. The DD genotype of ACE is associated with high PAI-1 level. The genetic variation of ACE contributes to the balance of fibrinolytic pathway, indicating the pathogenesis mechanisms linking to the ACE I/D genotype and MI.     

Effect of repeated measles vaccine injection on interleukin-12,interleukin-13 levels in asthmatic children

AIM and METHODS:To study the immunological effect of measles vaccine therapy on asthmatic children, we examined the changes of interleukin-12 , interleukin-13 and total serum IgE levels in plasma and cultured peripheral blood mononuclear cells(PBMC) supernatant by means of ELISA in 13 mild-moderate asthmatic children treated with measles vaccine. Results were compared with 12 anti-symptomatic treatment mild-moderate asthmatic children and 17 normal children control group.RESULTS:After measles vaccine treatment, IL-13 and total serum IgE levels decreased remarkably, statistically lower than that of group receiving only anti-symptomatic treatment. There was no statistical difference in IL-12 level between the two group. Correlation analysis: 1)IL-12 level of plasma was negatively correlated to the level of serum total IgE, there was no correlation of supernatant IL-12 in PBMC to the total serum IgE; 2)IL-13 levels in plasma and PBMC were positively correlated to the level of total serum IgE; 3) IL-12 level was negatively correlated to IL-13.CONCLUSION:Measles vaccine could down-regulate IL-13 level,therefore decrease total IgE synthesis,but not affect IL-12 level in asthmatic children.     

Association study of genetic polymorphisms of ACE-ID and AT1R -A1166C with non-alcoholic steatohepatitis

AIM: To investigate the relationship between genetic polymorphisms of angiotensin converting enzyme (ACE) and angiotensin II type 1 receptor (AT1R), and non-alcoholic steatohepatitis (NASH) in Kochi area of Japan.METHODS: In 104 patients with biopsy-proven NASH and 150 healthy volunteers, the genetic polymorphisms in ACE-ID and AT1R -A1166C were determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). RESULTS: The D-allele of the ACE-ID polymorphism was more significantly frequent in NASH patients than that in healthy volunteers (P<0.01). The DD-genotype of ACE-ID was more significantly frequent in NASH patients than that in healthy volunteers (P<0.01). The A-allele and the AA-genotype of AT1R -A1166C polymorphism was not significantly different in the genotype distribution in NASH patients than that in healthy volunteers (P>0.05). CONCLUSION: The D-allele and DD-genotype of ACE-ID polymorphism is associated with an increased risk of NASH. The AT1R -A1166C polymorphism of AT1R is not correlated with NASH.     

Effects of ginsenoside Rh1 on expression of inflammatory factors in mouse asthma model

AIM: To observe the effects of ginsenoside Rh1 on the levels of inflammatory factors in serum and bronchoalveolar lavage fluid (BALF), and the pathological changes of the lung tissues in an experimentally induced mouse asthma model. METHODS: Male BALB/c mice (n=40) were divided into 4 groups:normal control group, asthma mo-del group, and low-dose (40 mg·kg^-1·d^-1) and high-dose (80 mg·kg^-1·d^-1) ginsenoside Rh1 groups. The bronchial asthma mouse model was established by the method of ovalbumin induction and excitation, and during the excitation period, the mice were daily treated with ginsenoside Rh1 for 2 weeks. At 24 h after the final dose of ginsenoside Rh1, the mice were sacrificed. The number of eosinophils (EOS) and the concentrations of interleukin (IL)-4, IL-5 and interferon (IFN)-γ in BALF were determined. The levels of IgG and IgE in serum were measured, and the expression of transforming growth factor (TGF)-β1 and the pathological changes in lung tissues were evaluated. RESULTS: Ginsenoside Rh1 inhibited the increases in the number of EOS and the concentrations of IL-4, IL-5, IFN-γ and IgE, reversed the increased expression of TGF-β1, and improved the pathological changes of the lung tissues in asthmatic mice. CONCLUSION: Ginsenoside Rh1 improves the immuno-inflammatory profile and pathological changes in the experimentally induced mouse asthma model, implying its potential therapeutic effect on asthma.     

Levels of IL-18, IL-16, IL-8, eotaxin and the chymase activity in the sputum of asthmatics

AIM: To investigate the levels of IL-18, IL-16, IL-8, eotaxin and the chymase activity in the sputum of asthmatics. METHODS: IL-18, IL-16, IL-8 and eotaxin levels were detected with sandwich ELISA procedures and chymase activity was determined spectrophotometrically (410 nm) by the rate of hydrolysis of N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide (SAAPP). RESULTS: The specific chymase activities in the severe and moderate asthmatics were higher than that in controls. Native protease inhibitors α1-antitrypsin (α1-AT) and soybean trypsin inhibitor (SBTI) inhibited 71.9% and 72.1% enzymatic chymase activity, respectively. The levels of IL-18, IL-16, IL-8 and eotaxin were significantly elevated in the sputum of patients with acute asthma. There were correlations between the levels of IL-8 and IL-16 (r=0.55, P<0.05), IL-8 and eotaxin (r=0.41, P<0.05), IL-18 and IL-16 (r=0.64, P<0.01), IL-18 and eotaxin (r=0.66, P<0.01)，IL-16 and eotaxin (r=0.64, P<0.01), but they all failed to correlate with neutrophils, epithelial cells, lymphocytes and macrophages. CONCLUSION: The levels of IL-18， IL-16， IL-8， eotaxin and the activity of chymase were elevated in the sputum of the patients with asthma, indicating that they may play a role in the pathogenesis of asthma.     

The relationship between high density lipoprotein subclasses and apoE gene polymorphism in obese subjects

AIM: The purpose of this study is to elucidate the relationship between apolipoprotein (apo) E polymorphism and plasma lipid profiles and HDL subclasses in obesity. METHODS: apoE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The subclasses of serum HDL in 93 obese subjects and 96 nonobese subjects were determined by two-dimensional gel electrophoresis conjunction with immunodetection method. RESULTS: apoE3/3 genotypes and allele ε3 frequency in obese group and the control group were both the highest, but no significant difference. In obese group, the genotype of apoE2 had higher serum apoE/aopCⅢ, HDL2a and lower apoB100, apoCⅢ, HDL3c levels compared with the genotype of apoE3 (P<0.05). In control group, the genotype of apoE2 had higher serum TC and apoE levels, but lower HDL3b level compared with the genotype of apoE3 (P<0.05). CONCLUSION: Polymorphism of the ApoE gene is associated with the distribution of HDL particles in obesity. Allele of ε2 carrier may slow the tendency of HDL particals shifted towards smaller sizes.     

Effect of IL-13 on expression of IL-1β in acute renal ischemia/ reperfusion injury in rats

AIM:To investigate the effects of IL-13 on expression of IL-1β in acute renal ischemia/reperfusion injury.METHODS:Fifty-seven male Wistar rats were randomly divided into 8 group: normal group, sham operation group, ischemia group, ischemia/reperfusion injury group(I/R), normal saline(NS)-treated group 1(C-1), NS-treated group 2(C-2), IL-13-treated group1(T-1)and IL-13-treated group 2(T-2).Rats were subjected to 45 min bilateral renal ischemia followed by reperfusion. rmIL-13 (1.5 μg/50 g body weight )was injected into the renal arteries through the abdominal aorta before ischemia(T-1) or immediately afterischemia(T-2).The serum level of IL-1β and the renal expression of IL-1β were determined in each group at 24 h post-ischemia. In addition, BUN, Cr and renal histology were also measured.RESULTS:(1)The serum level of IL-1β, gene expression and protein production of IL-1β in kidney decreased markedly in IL-13-treated groups.(2)Renal function and histology were significantly improved in IL-13-treated groups, renal injury scores decreased significantly.(3)A positive correlation were found between the serum level of IL-1β and BUN, SCr(r=0.708, P＜0.01;r=0.770, P＜0.01).CONCLUSION:These data suggest that IL-13 inhibit the expression of IL-1βand improve func-tion and histology of kidney in acute renal ischemia/reperfusion injury.